pycom15g24560

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012565
Physical Location & Seq
Forward (+)
18779400 .. 18781574
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 999 bp
ATGGCTCGGAATGTTAAGAACGCCAAGCCCCAAACGCCGCTGTCCAAGGAGGACGGCCACCTGACTCCGCCTCCATTGCCCAAACCCAGGCTCCGCCCCAAGCCCGCCACCAACCCTAAACCTTCTTCTCTCCCCACGGGGAAGTTGAAGAAGCCAGGGAAGCCAAAAGAGCAAGAAGTGGAGTATTCCATGCCTATGAAGGAGCTTTCTAATACCCAAATTACAAAGATGTTGAGTCATGCAGGGAAGTCCCTGGCCATTAGACGCAGTATGATTTTCCAGTTGATGGATCGACCCTCACGAGGTCAAGCAAAGTCATCTGCTGTCATTCGAGCGCAGGAAGTTCGATCAAAACTAGAACCTCAATTCCCCAGCTTTGTGAAATCACTGGTCAGATCACACGTGGCTAGTTGTTTTTGGATGGGGCTTCCTTCGCTGTTCTGCAAATCACACTTACCAGATAAAGATATTACAATGACTTTGGAAGATGAAAGTGGTAGACAGTATCAGTCGAAATACATTGCATGCAAGACTGGACTAAGCGCTGGTTGGAGGCAGTTTGCTGCTTCGCACAATTTGCTCGAGGGAGATGTTTTGGCCTTCCAATTAGTTGAACCTACAAAATTTAAGGTTTACATAATAAGGTCAAATGGTTTGGCTGAAGTTGATGGGGATCTTGGCCTTCCAAATTTGGATAATAATACAAAACAAAAGGATGCAGATAAAGATAATGCAGAAACGAGCACTACACCTTGTAATGACTCTACCAAGAAACGTGCAGCAACAAGCACTATGCCTTGGAATGACTCTAATAGGAAACGCGCACATTCTCCTCAATTAGTAGTTCCAAAGAAGAAAAAGATTGGTTTGCAGCAAGTCAAACAATCTGAAACCGATAGTGAGGAGGTTGGTTCTGAAGTTTCAGAAGATCGATCAAGTCACCTGCTGCAACTCTTCAATTTGAGGACATCCGAAGTTATGAGAATTTCAACATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

333

Amino Acids

37.07

Weight (kDa)

9.74

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 126 - 216 1.3e-17 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 951
Acc36I ACCTGC 1 cut(s) 951
AccB7I CCANNNNNTGG 1 cut(s) 286
AccI GTMKAC 1 cut(s) 499
AccII CGCG 1 cut(s) 822
AciI CCGC 4 cut(s) 38, 68, 94, 105
AclWI GGATC 2 cut(s) 297, 681
AcoI YGGCCR 2 cut(s) 55, 255
AcsI RAATTY 3 cut(s) 623, 688, 984
AcuI CTGAAG 2 cut(s) 681, 936
AcvI CACGTG 1 cut(s) 403
AfeI AGCGCT 1 cut(s) 544
AfiI CCNNNNNNNGG 3 cut(s) 87, 286, 302
AflIII ACRYGT 1 cut(s) 400
AgsI TTSAA 4 cut(s) 148, 614, 958, 990
AjnI CCWGG 3 cut(s) 86, 154, 252
AloI GAACNNNNNNTCC 2 cut(s) 351, 383
AluBI AGCT 2 cut(s) 205, 375
AluI AGCT 2 cut(s) 205, 375
Alw21I GWGCWC 1 cut(s) 746
AlwI GGATC 2 cut(s) 297, 681
Ama87I CYCGRG 1 cut(s) 581
Aor51HI AGCGCT 1 cut(s) 544
AoxI GGCC 4 cut(s) 55, 255, 597, 679
ApeKI GCWGC 4 cut(s) 563, 779, 871, 946
ApoI RAATTY 3 cut(s) 623, 688, 984
AspLEI GCGC 3 cut(s) 337, 545, 824
AsuHPI GGTGA 1 cut(s) 932
AvaI CYCGRG 1 cut(s) 581
BalI TGGCCA 1 cut(s) 257
BauI CACGAG 1 cut(s) 300
BbrPI CACGTG 1 cut(s) 403
Bbv12I GWGCWC 1 cut(s) 746
BbvI GCAGC 4 cut(s) 550, 791, 883, 933
BccI CCATC 3 cut(s) 280, 415, 662
BceAI ACGGC 1 cut(s) 70
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BciT130I CCWGG 3 cut(s) 88, 156, 254
BfaI CTAG 3 cut(s) 356, 408, 997
BfoI RGCGCY 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 951
BisI GCNGC 5 cut(s) 38, 564, 780, 872, 947
BlsI GCNGC 5 cut(s) 39, 565, 781, 873, 948
Bme1390I CCNGG 3 cut(s) 88, 156, 254
BmeT110I CYCGRG 1 cut(s) 581
BmiI GGNNCC 1 cut(s) 92
BmrFI CCNGG 3 cut(s) 88, 156, 254
BmsI GCATC 1 cut(s) 706
Bsa29I ATCGAT 1 cut(s) 931
BsaAI YACGTR 1 cut(s) 403
BsaBI GATNNNNATC 1 cut(s) 672
BsaJI CCNNGG 6 cut(s) 45, 86, 135, 155, 252, 797
BsaXI ACNNNNNCTCC 4 cut(s) 55, 75, 85, 105
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 286, 302
Bse1I ACTGG 3 cut(s) 280, 393, 538
Bse3DI GCAATG 2 cut(s) 74, 519
Bse8I GATNNNNATC 1 cut(s) 672
BseBI CCWGG 3 cut(s) 88, 156, 254
BseCI ATCGAT 1 cut(s) 931
BseDI CCNNGG 6 cut(s) 45, 86, 135, 155, 252, 797
BseGI GGATG 3 cut(s) 426, 721, 968
BseJI GATNNNNATC 1 cut(s) 672
BseLI CCNNNNNNNGG 3 cut(s) 87, 286, 302
BseMI GCAATG 2 cut(s) 74, 519
BseNI ACTGG 3 cut(s) 280, 393, 538
BseRI GAGGAG 2 cut(s) 822, 917
BseXI GCAGC 4 cut(s) 550, 791, 883, 933
BseYI CCCAGC 1 cut(s) 371
BsgI GTGCAG 1 cut(s) 798
Bsh1236I CGCG 1 cut(s) 822
BshFI GGCC 4 cut(s) 57, 257, 599, 681
BshVI ATCGAT 1 cut(s) 931
BsiHKAI GWGCWC 1 cut(s) 746
BsiHKCI CYCGRG 1 cut(s) 581
BslFI GGGAC 1 cut(s) 235
BslI CCNNNNNNNGG 3 cut(s) 87, 286, 302
BsmFI GGGAC 1 cut(s) 235
BsnI GGCC 4 cut(s) 57, 257, 599, 681
BsoBI CYCGRG 1 cut(s) 581
Bsp1286I GDGCHC 1 cut(s) 746
Bsp143I GATC 6 cut(s) 289, 347, 395, 673, 928, 932
BspACI CCGC 4 cut(s) 38, 68, 94, 105
BspANI GGCC 4 cut(s) 57, 257, 599, 681
BspDI ATCGAT 1 cut(s) 931
BspFNI CGCG 1 cut(s) 822
BspLI GGNNCC 1 cut(s) 92
BspMI ACCTGC 1 cut(s) 951
BspPI GGATC 2 cut(s) 297, 681
BsrDI GCAATG 2 cut(s) 74, 519
BsrI ACTGG 3 cut(s) 280, 393, 538
BssECI CCNNGG 6 cut(s) 45, 86, 135, 155, 252, 797
BssMI GATC 6 cut(s) 289, 347, 395, 673, 928, 932
BssSI CACGAG 1 cut(s) 300
BssT1I CCWWGG 2 cut(s) 45, 797
Bst2BI CACGAG 1 cut(s) 300
Bst2UI CCWGG 3 cut(s) 88, 156, 254
Bst4CI ACNGT 1 cut(s) 504
Bst6I CTCTTC 1 cut(s) 959
BstAPI GCANNNNNTGC 1 cut(s) 577
BstBAI YACGTR 1 cut(s) 403
BstC8I GCNNGC 2 cut(s) 105, 526
BstDEI CTNAG 1 cut(s) 539
BstDSI CCRYGG 1 cut(s) 135
BstF5I GGATG 3 cut(s) 426, 721, 968
BstFNI CGCG 1 cut(s) 822
BstH2I RGCGCY 1 cut(s) 546
BstHHI GCGC 3 cut(s) 337, 545, 824
BstKTI GATC 6 cut(s) 292, 350, 398, 676, 931, 935
BstMBI GATC 6 cut(s) 289, 347, 395, 673, 928, 932
BstMWI GCNNNNNNNGC 6 cut(s) 34, 76, 160, 169, 433, 577
BstNI CCWGG 3 cut(s) 88, 156, 254
BstNSI RCATGY 1 cut(s) 528
BstSCI CCNGG 3 cut(s) 86, 154, 252
BstUI CGCG 1 cut(s) 822
BstV1I GCAGC 4 cut(s) 550, 791, 883, 933
BstX2I RGATCY 1 cut(s) 673
BstYI RGATCY 1 cut(s) 673
Bsu15I ATCGAT 1 cut(s) 931
BsuRI GGCC 4 cut(s) 57, 257, 599, 681
BsuTUI ATCGAT 1 cut(s) 931
BtgI CCRYGG 1 cut(s) 135
BtsCI GGATG 3 cut(s) 426, 721, 968
BtsIMutI CAGTG 1 cut(s) 386
BveI ACCTGC 1 cut(s) 951
Cac8I GCNNGC 2 cut(s) 105, 526
CfoI GCGC 3 cut(s) 337, 545, 824
ClaI ATCGAT 1 cut(s) 931
CseI GACGC 1 cut(s) 273
CviAII CATG 3 cut(s) 190, 239, 525
DdeI CTNAG 1 cut(s) 539
DpnI GATC 6 cut(s) 291, 349, 397, 675, 930, 934
DpnII GATC 6 cut(s) 289, 347, 395, 673, 928, 932
EaeI YGGCCR 2 cut(s) 55, 255
Eam1104I CTCTTC 1 cut(s) 959
EarI CTCTTC 1 cut(s) 959
EciI GGCGGA 2 cut(s) 57, 83
Eco130I CCWWGG 2 cut(s) 45, 797
Eco47III AGCGCT 1 cut(s) 544
Eco57I CTGAAG 2 cut(s) 681, 936
Eco72I CACGTG 1 cut(s) 403
Eco88I CYCGRG 1 cut(s) 581
EcoRII CCWGG 3 cut(s) 86, 154, 252
EcoT14I CCWWGG 2 cut(s) 45, 797
ErhI CCWWGG 2 cut(s) 45, 797
FaeI CATG 3 cut(s) 193, 242, 528
FaiI YATR 8 cut(s) 191, 197, 240, 272, 526, 638, 794, 980
FaqI GGGAC 1 cut(s) 235
FatI CATG 3 cut(s) 189, 238, 524
FauI CCCGC 1 cut(s) 112
FblI GTMKAC 1 cut(s) 499
Fnu4HI GCNGC 5 cut(s) 38, 564, 780, 872, 947
FokI GGATG 3 cut(s) 433, 728, 955
Fsp4HI GCNGC 5 cut(s) 38, 564, 780, 872, 947
FspBI CTAG 3 cut(s) 356, 408, 997
GlaI GCGC 3 cut(s) 336, 544, 823
GluI GCNGC 5 cut(s) 38, 564, 780, 872, 947
GsaI CCCAGC 1 cut(s) 375
HaeII RGCGCY 1 cut(s) 546
HaeIII GGCC 4 cut(s) 57, 257, 599, 681
HgaI GACGC 1 cut(s) 273
HhaI GCGC 3 cut(s) 337, 545, 824
Hin1II CATG 3 cut(s) 193, 242, 528
Hin6I GCGC 3 cut(s) 335, 543, 822
HinP1I GCGC 3 cut(s) 335, 543, 822
HinfI GANTC 4 cut(s) 64, 235, 761, 806
HphI GGTGA 1 cut(s) 932
Hpy166II GTNNAC 2 cut(s) 500, 634
Hpy188I TCNGA 6 cut(s) 9, 395, 889, 916, 925, 973
Hpy188III TCNNGA 1 cut(s) 300
Hpy8I GTNNAC 2 cut(s) 500, 634
HpyAV CCTTC 5 cut(s) 132, 193, 441, 610, 692
HpyCH4III ACNGT 1 cut(s) 504
HpyCH4IV ACGT 2 cut(s) 402, 775
HpyCH4V TGCA 9 cut(s) 242, 444, 524, 528, 719, 734, 779, 871, 949
HpyF10VI GCNNNNNNNGC 6 cut(s) 34, 76, 160, 169, 433, 577
HpyF3I CTNAG 1 cut(s) 539
HpySE526I ACGT 2 cut(s) 402, 775
Hsp92II CATG 3 cut(s) 193, 242, 528
HspAI GCGC 3 cut(s) 335, 543, 822
Kzo9I GATC 6 cut(s) 289, 347, 395, 673, 928, 932
LmnI GCTCC 2 cut(s) 96, 202
Lsp1109I GCAGC 4 cut(s) 550, 791, 883, 933
LweI GCATC 1 cut(s) 706
MaeI CTAG 3 cut(s) 356, 408, 997
MaeII ACGT 2 cut(s) 402, 775
MaeIII GTNAC 1 cut(s) 938
MalI GATC 6 cut(s) 291, 349, 397, 675, 930, 934
MboI GATC 6 cut(s) 289, 347, 395, 673, 928, 932
MboII GAAGA 6 cut(s) 117, 160, 497, 865, 938, 946
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 1 cut(s) 746
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 9 cut(s) 219, 365, 574, 605, 623, 688, 836, 958, 984
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 4 cut(s) 58, 244, 755, 800
MmeI TCCRAC 1 cut(s) 530
Mox20I TGGCCA 1 cut(s) 257
MscI TGGCCA 1 cut(s) 257
MseI TTAA 2 cut(s) 15, 627
MslI CAYNNNNRTG 1 cut(s) 194
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 40
MspR9I CCNGG 3 cut(s) 88, 156, 254
MvaI CCWGG 3 cut(s) 88, 156, 254
MvnI CGCG 1 cut(s) 822
MwoI GCNNNNNNNGC 6 cut(s) 34, 76, 160, 169, 433, 577
NdeII GATC 6 cut(s) 289, 347, 395, 673, 928, 932
NlaIII CATG 3 cut(s) 193, 242, 528
NlaIV GGNNCC 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 938
NspI RCATGY 1 cut(s) 528
PaeI GCATGC 1 cut(s) 528
PaeR7I CTCGAG 1 cut(s) 581
PaqCI CACCTGC 1 cut(s) 951
PcsI WCGNNNNNNNCGW 1 cut(s) 298
PflMI CCANNNNNTGG 1 cut(s) 286
PkrI GCNGC 5 cut(s) 39, 565, 781, 873, 948
PleI GAGTC 4 cut(s) 58, 243, 755, 800
PmaCI CACGTG 1 cut(s) 403
PmlI CACGTG 1 cut(s) 403
PpsI GAGTC 4 cut(s) 58, 243, 755, 800
Ppu21I YACGTR 1 cut(s) 403
Psp6I CCWGG 3 cut(s) 86, 154, 252
PspCI CACGTG 1 cut(s) 403
PspFI CCCAGC 1 cut(s) 371
PspGI CCWGG 3 cut(s) 86, 154, 252
PspN4I GGNNCC 1 cut(s) 92
PspXI VCTCGAGB 1 cut(s) 581
PsuI RGATCY 1 cut(s) 673
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 2 cut(s) 15, 627
SatI GCNGC 5 cut(s) 38, 564, 780, 872, 947
Sau3AI GATC 6 cut(s) 289, 347, 395, 673, 928, 932
SchI GAGTC 4 cut(s) 58, 244, 755, 800
ScrFI CCNGG 3 cut(s) 88, 156, 254
SduI GDGCHC 1 cut(s) 746
SfaNI GCATC 1 cut(s) 706
Sfr274I CTCGAG 1 cut(s) 581
SlaI CTCGAG 1 cut(s) 581
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
SphI GCATGC 1 cut(s) 528
Sse9I AATT 9 cut(s) 219, 365, 574, 605, 623, 688, 836, 958, 984
SsiI CCGC 4 cut(s) 38, 68, 94, 105
SspMI CTAG 3 cut(s) 356, 408, 997
StyD4I CCNGG 3 cut(s) 86, 154, 252
StyI CCWWGG 2 cut(s) 45, 797
TaaI ACNGT 1 cut(s) 504
TaiI ACGT 2 cut(s) 405, 778
TaqI TCGA 6 cut(s) 292, 331, 346, 512, 582, 931
TasI AATT 9 cut(s) 219, 365, 574, 605, 623, 688, 836, 958, 984
TauI GCSGC 1 cut(s) 40
Tru1I TTAA 2 cut(s) 15, 627
Tru9I TTAA 2 cut(s) 15, 627
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 938
TseI GCWGC 4 cut(s) 563, 779, 871, 946
Tsp45I GTSAC 1 cut(s) 938
TspDTI ATGAA 2 cut(s) 212, 504
TspRI CASTG 1 cut(s) 393
Van91I CCANNNNNTGG 1 cut(s) 286
XapI RAATTY 3 cut(s) 623, 688, 984
XceI RCATGY 1 cut(s) 528
XhoI CTCGAG 1 cut(s) 581
XmiI GTMKAC 1 cut(s) 499
XspI CTAG 3 cut(s) 356, 408, 997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.