pycom15g24570

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012565
Physical Location & Seq
Forward (+)
18781682 .. 18782080
399 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 399 bp
ATGAATCATAATTTGATTATAGGAGCCATATCTGAAACCGTAAATATTGCTGATTCCATTAAAGCTTGCAAGGTCACTGCGCTTCGAGATGATTTTATGTCTTGGGACAAGTATTTAACGGCCTTTGAGTTGTTGGGCATGAATGTTGGATTTTTACGTGAACGGGTATGTCGGCTTGCAAACCTTGCCGACGACTCGGAAGCTGCCATGGAGGCAAAGATGTATAAAGAAGCTAGAATTGAGCGATCTCAGGCAGGAATAGGAATAAGAAATATCGAGCTGAAGCTGGAGGAACTGATGGAGGCGTGCGATGAATTTGGTGCTGCCATTGAGAGCCTGAGATCGAAAGCTGAAATCAACAAGCTCAGGTTCCAGGAAGAGGTTACCGCTCCTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

133

Amino Acids

14.92

Weight (kDa)

4.9

Isoelectric Point (pI)

66.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 389
AciI CCGC 1 cut(s) 387
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 1 cut(s) 302
AfiI CCNNNNNNNGG 2 cut(s) 379, 393
AjnI CCWGG 1 cut(s) 372
AluBI AGCT 7 cut(s) 65, 203, 233, 280, 286, 350, 364
AluI AGCT 7 cut(s) 65, 203, 233, 280, 286, 350, 364
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 2 cut(s) 203, 323
ApoI RAATTY 1 cut(s) 314
AspLEI GCGC 1 cut(s) 82
BbvI GCAGC 2 cut(s) 190, 310
BccI CCATC 1 cut(s) 292
BceAI ACGGC 1 cut(s) 135
BciT130I CCWGG 1 cut(s) 374
BfaI CTAG 1 cut(s) 234
BglI GCCNNNNNGGC 1 cut(s) 212
BisI GCNGC 2 cut(s) 204, 324
BlsI GCNGC 2 cut(s) 205, 325
Bme1390I CCNGG 1 cut(s) 374
BmiI GGNNCC 2 cut(s) 25, 371
BmrFI CCNGG 1 cut(s) 374
BpmI CTGGAG 1 cut(s) 308
Bpu10I CCTNAGC 1 cut(s) 365
BsaAI YACGTR 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 207, 392
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 393
BseBI CCWGG 1 cut(s) 374
BseDI CCNNGG 2 cut(s) 207, 392
BseLI CCNNNNNNNGG 2 cut(s) 379, 393
BseMII CTCAG 3 cut(s) 263, 329, 379
BseXI GCAGC 2 cut(s) 190, 310
BshFI GGCC 1 cut(s) 122
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 2 cut(s) 379, 393
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 1 cut(s) 122
Bsp143I GATC 2 cut(s) 245, 341
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 1 cut(s) 387
BspANI GGCC 1 cut(s) 122
BspCNI CTCAG 3 cut(s) 262, 330, 378
BspLI GGNNCC 2 cut(s) 25, 371
BsrBI CCGCTC 1 cut(s) 389
BssECI CCNNGG 2 cut(s) 207, 392
BssMI GATC 2 cut(s) 245, 341
BssT1I CCWWGG 2 cut(s) 207, 392
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 1 cut(s) 40
Bst6I CTCTTC 1 cut(s) 372
BstAPI GCANNNNNTGC 1 cut(s) 185
BstBAI YACGTR 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 67, 177, 307
BstDEI CTNAG 3 cut(s) 249, 338, 365
BstDSI CCRYGG 1 cut(s) 207
BstEII GGTNACC 1 cut(s) 382
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 2 cut(s) 248, 344
BstMBI GATC 2 cut(s) 245, 341
BstMWI GCNNNNNNNGC 2 cut(s) 185, 212
BstNI CCWGG 1 cut(s) 374
BstPI GGTNACC 1 cut(s) 382
BstSCI CCNGG 1 cut(s) 372
BstV1I GCAGC 2 cut(s) 190, 310
BsuRI GGCC 1 cut(s) 122
BtgI CCRYGG 1 cut(s) 207
BtgZI GCGATG 1 cut(s) 324
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 3 cut(s) 67, 177, 307
CfoI GCGC 1 cut(s) 82
CviAII CATG 2 cut(s) 139, 208
DdeI CTNAG 3 cut(s) 249, 338, 365
DpnI GATC 2 cut(s) 247, 343
DpnII GATC 2 cut(s) 245, 341
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
Eco130I CCWWGG 2 cut(s) 207, 392
Eco57I CTGAAG 1 cut(s) 302
Eco91I GGTNACC 1 cut(s) 382
EcoO65I GGTNACC 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 372
EcoT14I CCWWGG 2 cut(s) 207, 392
ErhI CCWWGG 2 cut(s) 207, 392
FaeI CATG 2 cut(s) 142, 211
FaiI YATR 8 cut(s) 9, 20, 29, 98, 140, 169, 209, 225
FaqI GGGAC 1 cut(s) 119
FatI CATG 2 cut(s) 138, 207
Fnu4HI GCNGC 2 cut(s) 204, 324
Fsp4HI GCNGC 2 cut(s) 204, 324
FspBI CTAG 1 cut(s) 234
GlaI GCGC 1 cut(s) 81
GluI GCNGC 2 cut(s) 204, 324
GsuI CTGGAG 1 cut(s) 308
HaeIII GGCC 1 cut(s) 122
HhaI GCGC 1 cut(s) 82
Hin1II CATG 2 cut(s) 142, 211
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HindIII AAGCTT 1 cut(s) 63
HinfI GANTC 3 cut(s) 4, 53, 194
Hpy166II GTNNAC 1 cut(s) 161
Hpy188I TCNGA 2 cut(s) 34, 199
Hpy188III TCNNGA 1 cut(s) 86
Hpy8I GTNNAC 1 cut(s) 161
Hpy99I CGWCG 1 cut(s) 194
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4IV ACGT 1 cut(s) 157
HpyCH4V TGCA 2 cut(s) 69, 179
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 212
HpyF3I CTNAG 3 cut(s) 249, 338, 365
HpySE526I ACGT 1 cut(s) 157
Hsp92II CATG 2 cut(s) 142, 211
HspAI GCGC 1 cut(s) 80
Kzo9I GATC 2 cut(s) 245, 341
LmnI GCTCC 2 cut(s) 23, 394
LpnPI CCDG 7 cut(s) 236, 240, 272, 350, 352, 359, 386
Lsp1109I GCAGC 2 cut(s) 190, 310
MaeI CTAG 1 cut(s) 234
MaeII ACGT 1 cut(s) 157
MaeIII GTNAC 2 cut(s) 73, 382
MalI GATC 2 cut(s) 247, 343
MbiI CCGCTC 1 cut(s) 389
MboI GATC 2 cut(s) 245, 341
MboII GAAGA 1 cut(s) 389
MluCI AATT 3 cut(s) 10, 237, 314
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 4 cut(s) 205, 283, 295, 373
MseI TTAA 2 cut(s) 60, 116
MspR9I CCNGG 1 cut(s) 374
MvaI CCWGG 1 cut(s) 374
MwoI GCNNNNNNNGC 2 cut(s) 185, 212
NcoI CCATGG 1 cut(s) 207
NdeII GATC 2 cut(s) 245, 341
NlaIII CATG 2 cut(s) 142, 211
NlaIV GGNNCC 2 cut(s) 25, 371
NmuCI GTSAC 1 cut(s) 73
PfeI GAWTC 2 cut(s) 4, 53
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 2 cut(s) 205, 325
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
Ppu21I YACGTR 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 372
PspEI GGTNACC 1 cut(s) 382
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 2 cut(s) 25, 371
SaqAI TTAA 2 cut(s) 60, 116
SatI GCNGC 2 cut(s) 204, 324
Sau3AI GATC 2 cut(s) 245, 341
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 1 cut(s) 374
Sse9I AATT 3 cut(s) 10, 237, 314
SsiI CCGC 1 cut(s) 387
SspI AATATT 1 cut(s) 46
SspMI CTAG 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 372
StyI CCWWGG 2 cut(s) 207, 392
TaaI ACNGT 1 cut(s) 40
TaiI ACGT 1 cut(s) 160
TaqI TCGA 3 cut(s) 85, 276, 344
TasI AATT 3 cut(s) 10, 237, 314
TfiI GAWTC 2 cut(s) 4, 53
Tru1I TTAA 2 cut(s) 60, 116
Tru9I TTAA 2 cut(s) 60, 116
TscAI CASTG 1 cut(s) 82
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 2 cut(s) 203, 323
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 3 cut(s) 17, 155, 327
TspRI CASTG 1 cut(s) 82
XapI RAATTY 1 cut(s) 314
XspI CTAG 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.