pycom15g27530

UPF0503 protein At3g09070, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010196
Physical Location & Seq
Reverse (-)
23024183 .. 23024458
276 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 276 bp
ATGTCACTGGACGAACCTCTGGCTTCTTGGGACAAGTATTTGGGTCTAAGAGCCCCTCACCTGAGGCTAAACTCGATGGTTTCAGTTATGGAAGAGAGCTGGGAAAGGAGTCCAGGCGGCTCAGCTCAGACCAAAGACTATTACAATTCGAAGCGGTGGCGGAGCTTCGAACGACCAAGTTTCGATCGATCTGCAATGGCGGAGGTTGATGAGCTAAAGTTGGGATCAAATGCTAGATTATCCCCTGCCACTTCAGAGTTGTTCTATGGGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

92

Amino Acids

10.36

Weight (kDa)

5.79

Isoelectric Point (pI)

73.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF740 PF05340 35 - 90 2e-08 Protein of unknown function (DUF740)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 117, 154, 160, 200
AclWI GGATC 1 cut(s) 232
AcuI CTGAAG 1 cut(s) 237
AjnI CCWGG 1 cut(s) 112
AluBI AGCT 4 cut(s) 99, 125, 165, 214
AluI AGCT 4 cut(s) 99, 125, 165, 214
AlwI GGATC 1 cut(s) 232
AoxI GGCC 1 cut(s) 270
AspS9I GGNCC 1 cut(s) 270
AsuHPI GGTGA 1 cut(s) 50
AsuII TTCGAA 2 cut(s) 149, 168
AxyI CCTNAGG 1 cut(s) 62
BanII GRGCYC 1 cut(s) 55
BccI CCATC 1 cut(s) 70
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 1 cut(s) 114
BfaI CTAG 2 cut(s) 234, 274
BisI GCNGC 1 cut(s) 118
BlpI GCTNAGC 1 cut(s) 121
BlsI GCNGC 1 cut(s) 119
Bme1390I CCNGG 1 cut(s) 114
BmgT120I GGNCC 1 cut(s) 270
BmiI GGNNCC 1 cut(s) 271
BmrFI CCNGG 1 cut(s) 114
Bpu1102I GCTNAGC 1 cut(s) 121
Bpu14I TTCGAA 2 cut(s) 149, 168
Bsa29I ATCGAT 1 cut(s) 187
Bse1I ACTGG 1 cut(s) 12
Bse21I CCTNAGG 1 cut(s) 62
Bse3DI GCAATG 1 cut(s) 201
BseBI CCWGG 1 cut(s) 114
BseCI ATCGAT 1 cut(s) 187
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 3 cut(s) 53, 135, 140
BseNI ACTGG 1 cut(s) 12
BseYI CCCAGC 1 cut(s) 99
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 1 cut(s) 272
BshVI ATCGAT 1 cut(s) 187
BsiEI CGRYCG 1 cut(s) 187
BslFI GGGAC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 44
BsnI GGCC 1 cut(s) 272
Bsp119I TTCGAA 2 cut(s) 149, 168
Bsp1286I GDGCHC 1 cut(s) 55
Bsp143I GATC 3 cut(s) 184, 188, 224
Bsp1720I GCTNAGC 1 cut(s) 121
BspACI CCGC 4 cut(s) 117, 154, 160, 200
BspANI GGCC 1 cut(s) 272
BspCNI CTCAG 3 cut(s) 54, 134, 139
BspDI ATCGAT 1 cut(s) 187
BspLI GGNNCC 1 cut(s) 271
BspPI GGATC 1 cut(s) 232
BspT104I TTCGAA 2 cut(s) 149, 168
BsrDI GCAATG 1 cut(s) 201
BsrI ACTGG 1 cut(s) 12
BssMI GATC 3 cut(s) 184, 188, 224
Bst2UI CCWGG 1 cut(s) 114
Bst6I CTCTTC 1 cut(s) 87
BstBI TTCGAA 2 cut(s) 149, 168
BstDEI CTNAG 4 cut(s) 47, 62, 121, 126
BstKTI GATC 3 cut(s) 187, 191, 227
BstMBI GATC 3 cut(s) 184, 188, 224
BstMCI CGRYCG 1 cut(s) 187
BstNI CCWGG 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 112
Bsu15I ATCGAT 1 cut(s) 187
Bsu36I CCTNAGG 1 cut(s) 62
BsuRI GGCC 1 cut(s) 272
BsuTUI ATCGAT 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 5
Cfr13I GGNCC 1 cut(s) 270
ClaI ATCGAT 1 cut(s) 187
CviJI RGCY 9 cut(s) 23, 53, 67, 99, 120, 125, 165, 214, 272
CviKI_1 RGCY 9 cut(s) 23, 53, 67, 99, 120, 125, 165, 214, 272
DdeI CTNAG 4 cut(s) 47, 62, 121, 126
DpnI GATC 3 cut(s) 186, 190, 226
DpnII GATC 3 cut(s) 184, 188, 224
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
EciI GGCGGA 2 cut(s) 175, 215
Eco24I GRGCYC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 237
Eco81I CCTNAGG 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 270
EcoRII CCWGG 1 cut(s) 112
EcoT38I GRGCYC 1 cut(s) 55
FaiI YATR 2 cut(s) 89, 267
FaqI GGGAC 1 cut(s) 44
Fnu4HI GCNGC 1 cut(s) 118
FriOI GRGCYC 1 cut(s) 55
Fsp4HI GCNGC 1 cut(s) 118
FspBI CTAG 2 cut(s) 234, 274
GluI GCNGC 1 cut(s) 118
GsaI CCCAGC 1 cut(s) 103
HaeIII GGCC 1 cut(s) 272
HinfI GANTC 1 cut(s) 109
HphI GGTGA 1 cut(s) 50
Hpy188I TCNGA 2 cut(s) 129, 256
HpyCH4V TGCA 1 cut(s) 194
HpyF3I CTNAG 4 cut(s) 47, 62, 121, 126
Kzo9I GATC 3 cut(s) 184, 188, 224
LmnI GCTCC 1 cut(s) 162
LpnPI CCDG 6 cut(s) 5, 74, 85, 99, 126, 258
MaeI CTAG 2 cut(s) 234, 274
MaeIII GTNAC 1 cut(s) 3
MalI GATC 3 cut(s) 186, 190, 226
MboI GATC 3 cut(s) 184, 188, 224
MboII GAAGA 1 cut(s) 104
MhlI GDGCHC 1 cut(s) 55
MluCI AATT 1 cut(s) 145
MlyI GAGTC 1 cut(s) 118
MnlI CCTC 4 cut(s) 27, 57, 66, 196
MspR9I CCNGG 1 cut(s) 114
MvaI CCWGG 1 cut(s) 114
NdeII GATC 3 cut(s) 184, 188, 224
NlaIV GGNNCC 1 cut(s) 271
NmuCI GTSAC 1 cut(s) 3
NspV TTCGAA 2 cut(s) 149, 168
PkrI GCNGC 1 cut(s) 119
Ple19I CGATCG 1 cut(s) 187
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 112
PspFI CCCAGC 1 cut(s) 99
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 1 cut(s) 271
PspPI GGNCC 1 cut(s) 270
PvuI CGATCG 1 cut(s) 187
SatI GCNGC 1 cut(s) 118
Sau3AI GATC 3 cut(s) 184, 188, 224
Sau96I GGNCC 1 cut(s) 270
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 1 cut(s) 55
SetI ASST 7 cut(s) 19, 63, 101, 127, 167, 207, 216
SfuI TTCGAA 2 cut(s) 149, 168
Sse9I AATT 1 cut(s) 145
SsiI CCGC 4 cut(s) 117, 154, 160, 200
SspMI CTAG 2 cut(s) 234, 274
StyD4I CCNGG 1 cut(s) 112
TaqI TCGA 5 cut(s) 74, 149, 168, 183, 187
TasI AATT 1 cut(s) 145
TauI GCSGC 1 cut(s) 120
TscAI CASTG 1 cut(s) 12
TseFI GTSAC 1 cut(s) 3
Tsp45I GTSAC 1 cut(s) 3
TspRI CASTG 1 cut(s) 12
XspI CTAG 2 cut(s) 234, 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.