Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0005595

Physical Location & Seq

Genomic Coordinates Forward (+)

23058405 .. 23067068

Gene Structure 8664 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2184 bp

ATGGTTCTTGCTGGTTCATTGCAATTGTCCCATGGATTGGGACTTTGCAAGAATCAGAGGTACAGAGAACAATTTAAGTGTGGAATACGAAGGGACAAGTTACATTCCTTAAACACCGGCCTTACATCAAGATTTGCATTTCAGAAGCAGGATTCTTGGAGTATCAGTTTATCAGACAATGTATACAGGCCCTTACATACTGTATCTCACAGATATCAAGCATTTAAGTGCCATTCCTTGCTAACATCAGGCCAACATGGCTTGAATGCAGCTGCAATGGCGTTGACAAGATCATATAATGCTTTGCAAGGTTGTCCTCTTGTATTCAAATTGGTTCCATCAGTTGGCCTCATTATTTTTTCCGTATGGGGTCTTCCACCACTTATGCGCCTGATCAGAAATCTCGTCCTTCATAAGAGTGATAGTAGTTGGAAAAAGAGCAATACGCATTATGTGACAACCTCATATATTCAACCTTTGCTGCTTTGGACAGGAGCAATTCTCATTTGCAGGGCATTGGATCCAGTCGTTCTATCTACAGAAGCTAGCCAGGCTGTTAAGAAACGGCTTTTGGATTTTATAAGATCATTGTCAACTGTTCTGGCATTTGCCTATTGTTTATCAAGTGCAATTCAACAAGCACAGAAATTTTTTATGGAGAGTGGTGACTCCAGTAATACAAGAAATATGGGTTTTCAATTTGCCGGGAAGGCTGTTTACTCTGCAGTATGGGTTGCTGCTGTTTCGTTATTCATGGAGTTGCTAGGTTTCTCTACCCAAAGGTGGCTTACTGCAGGAGGTCTTGGGACAGTACTATTGACTCTGGCTGGTCGTGAGATCTTCACCAATTTCCTGTCAAGTGTAATGATTCATGCGACTCGCCCTTTTGTTGCAAATGAATGGATTCAAACAAAAATCGAAGGCTATGATGTATCTGGAACTGTTGAGCATGTGGGTTGGTGGTCACCAACAATCATAAGAGGTGAAGATCGTGAAGCAGTTCACATTCCAAACCACCAGTTCACAGTGAATGTTGTGAGAAATCTCAGTCAAAAAACTCATTGGCGTATCAAAACTCACCTTGCCATCAGTCATTTGGATGTCCATAAGATAAATAATATTGTTGCAGACATGCGCAAAGTCTTGTCCAAGAACCCTCAAGTTGAGCAGCAGAGGTTGCATAGAAGAGTATTTCTGGATAATGTTACCCCTGAAAACCAAGCTCTTTTGATCTTGATTTCCTGTTTTGTGAAGACATCACATTATGAAGAGTATCTGTGCGTTAAGGAAGCTATAATGTTGGATCTTCTTAGAGTCATTAGCCATCACAAGGCCCGACTTGCCACACCAATTCGTACAGTTCAGAAAATGTATAGCGATCCCGATTTGGACAGTGTGCCATTTGCAGATTCAATGTATAACCATGCTGGAGCAAGATCCAGACGCCCATTCATACTGATTGAACCCTCATACAAAATCAATGGAGAAGATAAAAAAAGGAGTCGTACGCAAACCAGTGGAGAGCGAGATGGGAAGGCCACAACGCGGCCATCTCCTGACTCCAAGGCCAGGGAAACACCAACTTATGACACCAAGTCAGATTCTAGAGCCGGAGCAACATCAGCATCTGACTCCAAGACCAGAGAAACACCAACATCTGATACCACAGCAGATGCCAAGGGTGGAGAAACGCCAAATTCAAACACAAAGGAGGACCCGAAGGGTGCAAAATCATCTACATCTAATCCCAAAGTTAGTAACAAAGAAACAGTGAAGTCAACATCTAATACTGTCTCCAAGTCAAATTCTAGAGACACTGAAAAGTCTGATTCTGACTCGAGAGCAACGGGCATAGCATCTGATAATTCAATGCAGAACTCTGACAGAAAGCAACAAAAGACTGCAAGTGGATCAACGGCTTCTCCAGTGAAACAGGAAGGTGAAAGGGCGCCCATTCCTGAGCCACCAATATCAAGGCCTGCTATAGAGGAAAACATAGTGCTTGGTGTTGCTTTAGAGGGCTCAAAGAGAACCCTTCCGATTGAGGAGGCGATGGCTTCCCCTTCAGCTCATGCAGAGGCAAACGATTTGGCTCCTTCCCACAATGGAACTGCATCTCCTATTAAGGGTAAGAAAAATGATCAGGTTCCGGCTGCACCAGGGTCAACATCTGGAAATGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009507 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010020 GO:0016043 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

728

Amino Acids

80.11

Weight (kDa)

9.54

Isoelectric Point (pI)

42.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	149 - 278	2.5e-70	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	280 - 350	2e-11	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	355 - 441	1.9e-45	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10490 AT5G10490 AT5G10490
fragaria_vesca	FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670
malus_domestica	MD15G1312300.v1.1
prunus_persica	Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1
pyrus_communis	pycom15g27600
rosa_chinensis	RchiOBHm_Chr2g0111221
rosa_laevigata	RLG00000017875
rosa_multiflora	Rmu_sc0003055.1_g000004
rosa_roxburghii	Rroxscaffold_2G00132610
rosa_rugosa	Rorug02G0171700 Rorug02G0171700 Rorug02G0171800
rosa_samantha	Rh2AG224800 Rh2BG236600 Rh2CG227800 Rh2DG232400
rosa_wichuraiana	Rw2G017360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	581
Acc16I	TGCGCA	1 cut(s)	1136
AccB1I	GGYRCC	1 cut(s)	1948
AccB7I	CCANNNNNTGG	2 cut(s)	37, 344
AccI	GTMKAC	1 cut(s)	183
AccII	CGCG	1 cut(s)	1546
AciI	CCGC	1 cut(s)	1546
AclWI	GGATC	6 cut(s)	515, 528, 1311, 1373, 1431, 1918
AcoI	YGGCCR	1 cut(s)	1547
AcsI	RAATTY	3 cut(s)	647, 1696, 1804
AcuI	CTGAAG	1 cut(s)	2049
AcyI	GRCGYC	2 cut(s)	1444, 1949
AfaI	GTAC	4 cut(s)	62, 813, 1357, 1507
AfiI	CCNNNNNNNGG	7 cut(s)	37, 344, 783, 1330, 1545, 1569, 2126
AhdI	GACNNNNNGTC	1 cut(s)	1594
AjnI	CCWGG	3 cut(s)	549, 1568, 2158
AjuI	GAANNNNNNNTTGG	2 cut(s)	554, 586
AloI	GAACNNNNNNTCC	2 cut(s)	2101, 2133
AluBI	AGCT	5 cut(s)	272, 545, 1223, 1292, 2069
AluI	AGCT	5 cut(s)	272, 545, 1223, 1292, 2069
Alw26I	GTCTC	2 cut(s)	1798, 1806
AlwI	GGATC	6 cut(s)	515, 528, 1311, 1373, 1431, 1918
AlwNI	CAGNNNCTG	1 cut(s)	1628
Ama87I	CYCGRG	1 cut(s)	1837
AoxI	GGCC	9 cut(s)	118, 188, 250, 346, 1332, 1536, 1547, 1566, 1976
ApeKI	GCWGC	6 cut(s)	269, 272, 481, 737, 1168, 2153
ApoI	RAATTY	3 cut(s)	647, 1696, 1804
Asp700I	GAANNNNTTC	2 cut(s)	903, 999
AspLEI	GCGC	3 cut(s)	390, 1137, 1951
AspS9I	GGNCC	3 cut(s)	189, 1333, 1714
AsuC2I	CCSGG	1 cut(s)	706
AsuHPI	GGTGA	6 cut(s)	677, 835, 957, 995, 1070, 1952
AsuNHI	GCTAGC	1 cut(s)	545
AvaI	CYCGRG	1 cut(s)	1837
AvaII	GGWCC	1 cut(s)	1714
BamHI	GGATCC	1 cut(s)	520
BanI	GGYRCC	1 cut(s)	1948
BanII	GRGCYC	1 cut(s)	2024
BarI	GAAGNNNNNNTAC	4 cut(s)	357, 389, 701, 733
BbsI	GAAGAC	2 cut(s)	365, 1259
BbvI	GCAGC	6 cut(s)	259, 281, 468, 724, 1180, 2140
BccI	CCATC	6 cut(s)	346, 1094, 1332, 1523, 1558, 2047
BceAI	ACGGC	2 cut(s)	581, 1932
BciT130I	CCWGG	3 cut(s)	551, 1570, 2160
BclI	TGATCA	2 cut(s)	393, 2140
BcnI	CCSGG	1 cut(s)	706
BcoDI	GTCTC	2 cut(s)	1798, 1806
BfaI	CTAG	4 cut(s)	546, 764, 1605, 1809
BfmI	CTRYAG	4 cut(s)	537, 723, 792, 1983
BfoI	RGCGCY	1 cut(s)	1952
BglI	GCCNNNNNGGC	2 cut(s)	258, 710
BglII	AGATCT	1 cut(s)	837
BisI	GCNGC	7 cut(s)	270, 273, 482, 738, 1169, 1547, 2154
BlsI	GCNGC	7 cut(s)	271, 274, 483, 739, 1170, 1548, 2155
BmcAI	AGTACT	1 cut(s)	813
Bme1390I	CCNGG	4 cut(s)	551, 706, 1570, 2160
Bme18I	GGWCC	1 cut(s)	1714
BmeRI	GACNNNNNGTC	1 cut(s)	1594
BmeT110I	CYCGRG	1 cut(s)	1837
BmgT120I	GGNCC	3 cut(s)	189, 1333, 1714
BmiI	GGNNCC	6 cut(s)	336, 522, 1716, 1950, 2094, 2148
BmrFI	CCNGG	4 cut(s)	551, 706, 1570, 2160
BmsI	GCATC	4 cut(s)	1634, 1663, 1865, 2123
BmtI	GCTAGC	1 cut(s)	549
BpiI	GAAGAC	2 cut(s)	365, 1259
BplI	GAGNNNNNCTC	2 cut(s)	486, 518
BpmI	CTGGAG	3 cut(s)	655, 1449, 1908
Bpu10I	CCTNAGC	1 cut(s)	1959
BpuEI	CTTGAG	1 cut(s)	1143
BpuMI	CCSGG	1 cut(s)	706
BsaHI	GRCGYC	2 cut(s)	1444, 1949
BsaJI	CCNNGG	5 cut(s)	31, 1563, 1569, 1677, 2159
BsaXI	ACNNNNNCTCC	6 cut(s)	151, 181, 1906, 1936, 2101, 2131
Bsc4I	CCNNNNNNNGG	7 cut(s)	37, 344, 783, 1330, 1545, 1569, 2126
Bse118I	RCCGGY	1 cut(s)	116
Bse1I	ACTGG	5 cut(s)	524, 672, 1018, 1515, 1925
Bse3DI	GCAATG	2 cut(s)	17, 282
BseBI	CCWGG	3 cut(s)	551, 1570, 2160
BseDI	CCNNGG	5 cut(s)	31, 1563, 1569, 1677, 2159
BseGI	GGATG	1 cut(s)	1105
BseLI	CCNNNNNNNGG	7 cut(s)	37, 344, 783, 1330, 1545, 1569, 2126
BseMI	GCAATG	2 cut(s)	17, 282
BseMII	CTCAG	2 cut(s)	1060, 1950
BseNI	ACTGG	5 cut(s)	524, 672, 1018, 1515, 1925
BseRI	GAGGAG	1 cut(s)	2060
BseXI	GCAGC	6 cut(s)	259, 281, 468, 724, 1180, 2140
BsgI	GTGCAG	1 cut(s)	2139
Bsh1236I	CGCG	1 cut(s)	1546
BshFI	GGCC	9 cut(s)	120, 190, 252, 348, 1334, 1538, 1549, 1568, 1978
BshNI	GGYRCC	1 cut(s)	1948
BsiHKCI	CYCGRG	1 cut(s)	1837
BsiSI	CCGG	4 cut(s)	117, 705, 1611, 2150
BsiWI	CGTACG	1 cut(s)	1505
BslFI	GGGAC	4 cut(s)	13, 54, 107, 820
BslI	CCNNNNNNNGG	7 cut(s)	37, 344, 783, 1330, 1545, 1569, 2126
BsmAI	GTCTC	2 cut(s)	1798, 1806
BsmFI	GGGAC	4 cut(s)	13, 54, 107, 820
BsmI	GAATGC	1 cut(s)	271
BsnI	GGCC	9 cut(s)	120, 190, 252, 348, 1334, 1538, 1549, 1568, 1978
BsoBI	CYCGRG	1 cut(s)	1837
Bsp1286I	GDGCHC	1 cut(s)	2024
Bsp19I	CCATGG	1 cut(s)	31
BspACI	CCGC	1 cut(s)	1546
BspANI	GGCC	9 cut(s)	120, 190, 252, 348, 1334, 1538, 1549, 1568, 1978
BspCNI	CTCAG	2 cut(s)	1059, 1951
BspFNI	CGCG	1 cut(s)	1546
BspLI	GGNNCC	6 cut(s)	336, 522, 1716, 1950, 2094, 2148
BspMAI	CTGCAG	2 cut(s)	727, 796
BspOI	GCTAGC	1 cut(s)	549
BspPI	GGATC	6 cut(s)	515, 528, 1311, 1373, 1431, 1918
BspT107I	GGYRCC	1 cut(s)	1948
BsrDI	GCAATG	2 cut(s)	17, 282
BsrFI	RCCGGY	1 cut(s)	116
BsrI	ACTGG	5 cut(s)	524, 672, 1018, 1515, 1925
BssAI	RCCGGY	1 cut(s)	116
BssECI	CCNNGG	5 cut(s)	31, 1563, 1569, 1677, 2159
BssNAI	GTATAC	1 cut(s)	184
BssNI	GRCGYC	2 cut(s)	1444, 1949
BssT1I	CCWWGG	3 cut(s)	31, 1563, 1677
Bst1107I	GTATAC	1 cut(s)	184
Bst2UI	CCWGG	3 cut(s)	551, 1570, 2160
Bst4CI	ACNGT	9 cut(s)	202, 598, 811, 943, 1027, 1360, 1394, 1771, 1792
Bst6I	CTCTTC	2 cut(s)	1180, 1263
BstACI	GRCGYC	2 cut(s)	1444, 1949
BstAPI	GCANNNNNTGC	1 cut(s)	1177
BstC8I	GCNNGC	2 cut(s)	547, 1980
BstDEI	CTNAG	3 cut(s)	1046, 1310, 1959
BstDSI	CCRYGG	1 cut(s)	31
BstEII	GGTNACC	1 cut(s)	963
BstF5I	GGATG	1 cut(s)	1105
BstFNI	CGCG	1 cut(s)	1546
BstH2I	RGCGCY	1 cut(s)	1952
BstHHI	GCGC	3 cut(s)	390, 1137, 1951
BstMAI	GTCTC	2 cut(s)	1798, 1806
BstMWI	GCNNNNNNNGC	7 cut(s)	258, 278, 551, 710, 1177, 1340, 1622
BstNI	CCWGG	3 cut(s)	551, 1570, 2160
BstNSI	RCATGY	2 cut(s)	953, 1135
BstPI	GGTNACC	1 cut(s)	963
BstSCI	CCNGG	4 cut(s)	549, 704, 1568, 2158
BstSFI	CTRYAG	4 cut(s)	537, 723, 792, 1983
BstUI	CGCG	1 cut(s)	1546
BstV1I	GCAGC	6 cut(s)	259, 281, 468, 724, 1180, 2140
BstV2I	GAAGAC	2 cut(s)	365, 1259
BstX2I	RGATCY	4 cut(s)	520, 837, 1303, 1436
BstYI	RGATCY	4 cut(s)	520, 837, 1303, 1436
BstZ17I	GTATAC	1 cut(s)	184
BsuRI	GGCC	9 cut(s)	120, 190, 252, 348, 1334, 1538, 1549, 1568, 1978
BtgI	CCRYGG	1 cut(s)	31
BtgZI	GCGATG	1 cut(s)	2066
BtsCI	GGATG	1 cut(s)	1105
BtsIMutI	CAGTG	6 cut(s)	1032, 1399, 1522, 1776, 1815, 1932
Cac8I	GCNNGC	2 cut(s)	547, 1980
CaiI	CAGNNNCTG	1 cut(s)	1628
CfoI	GCGC	3 cut(s)	390, 1137, 1951
Cfr10I	RCCGGY	1 cut(s)	116
Cfr13I	GGNCC	3 cut(s)	189, 1333, 1714
CseI	GACGC	1 cut(s)	1452
Csp6I	GTAC	4 cut(s)	61, 812, 1356, 1506
CviAII	CATG	8 cut(s)	32, 257, 754, 872, 950, 1132, 1424, 2072
CviQI	GTAC	4 cut(s)	61, 812, 1356, 1506
DdeI	CTNAG	3 cut(s)	1046, 1310, 1959
DinI	GGCGCC	1 cut(s)	1950
DriI	GACNNNNNGTC	1 cut(s)	1594
EaeI	YGGCCR	1 cut(s)	1547
Eam1104I	CTCTTC	2 cut(s)	1180, 1263
Eam1105I	GACNNNNNGTC	1 cut(s)	1594
EarI	CTCTTC	2 cut(s)	1180, 1263
Eco130I	CCWWGG	3 cut(s)	31, 1563, 1677
Eco147I	AGGCCT	1 cut(s)	1978
Eco24I	GRGCYC	1 cut(s)	2024
Eco32I	GATATC	1 cut(s)	215
Eco47I	GGWCC	1 cut(s)	1714
Eco57I	CTGAAG	1 cut(s)	2049
Eco88I	CYCGRG	1 cut(s)	1837
Eco91I	GGTNACC	1 cut(s)	963
EcoO109I	RGGNCCY	2 cut(s)	189, 1714
EcoO65I	GGTNACC	1 cut(s)	963
EcoRII	CCWGG	3 cut(s)	549, 1568, 2158
EcoRV	GATATC	1 cut(s)	215
EcoT14I	CCWWGG	3 cut(s)	31, 1563, 1677
EcoT38I	GRGCYC	1 cut(s)	2024
EgeI	GGCGCC	1 cut(s)	1950
EheI	GGCGCC	1 cut(s)	1950
ErhI	CCWWGG	3 cut(s)	31, 1563, 1677
FaeI	CATG	8 cut(s)	35, 260, 757, 875, 953, 1135, 1427, 2075
FaqI	GGGAC	4 cut(s)	13, 54, 107, 820
FatI	CATG	8 cut(s)	31, 256, 753, 871, 949, 1131, 1423, 2071
FbaI	TGATCA	2 cut(s)	393, 2140
FblI	GTMKAC	1 cut(s)	183
Fnu4HI	GCNGC	7 cut(s)	270, 273, 482, 738, 1169, 1547, 2154
FokI	GGATG	1 cut(s)	1112
FriOI	GRGCYC	1 cut(s)	2024
Fsp4HI	GCNGC	7 cut(s)	270, 273, 482, 738, 1169, 1547, 2154
FspBI	CTAG	4 cut(s)	546, 764, 1605, 1809
FspI	TGCGCA	1 cut(s)	1136
GlaI	GCGC	3 cut(s)	389, 1136, 1950
GluI	GCNGC	7 cut(s)	270, 273, 482, 738, 1169, 1547, 2154
GsuI	CTGGAG	3 cut(s)	655, 1449, 1908
HaeII	RGCGCY	1 cut(s)	1952
HaeIII	GGCC	9 cut(s)	120, 190, 252, 348, 1334, 1538, 1549, 1568, 1978
HapII	CCGG	4 cut(s)	117, 705, 1611, 2150
HgaI	GACGC	1 cut(s)	1452
HhaI	GCGC	3 cut(s)	390, 1137, 1951
Hin1I	GRCGYC	2 cut(s)	1444, 1949
Hin1II	CATG	8 cut(s)	35, 260, 757, 875, 953, 1135, 1427, 2075
Hin6I	GCGC	3 cut(s)	388, 1135, 1949
HinP1I	GCGC	3 cut(s)	388, 1135, 1949
HincII	GTYRAC	4 cut(s)	285, 594, 1779, 2166
HindII	GTYRAC	4 cut(s)	285, 594, 1779, 2166
HpaII	CCGG	4 cut(s)	117, 705, 1611, 2150
HphI	GGTGA	6 cut(s)	677, 835, 957, 995, 1070, 1952
Hpy166II	GTNNAC	8 cut(s)	184, 285, 594, 718, 1003, 1023, 1779, 2166
Hpy8I	GTNNAC	8 cut(s)	184, 285, 594, 718, 1003, 1023, 1779, 2166
HpyCH4III	ACNGT	9 cut(s)	202, 598, 811, 943, 1027, 1360, 1394, 1771, 1792
HpyF10VI	GCNNNNNNNGC	7 cut(s)	258, 278, 551, 710, 1177, 1340, 1622
HpyF3I	CTNAG	3 cut(s)	1046, 1310, 1959
Hsp92I	GRCGYC	2 cut(s)	1444, 1949
Hsp92II	CATG	8 cut(s)	35, 260, 757, 875, 953, 1135, 1427, 2075
HspAI	GCGC	3 cut(s)	388, 1135, 1949
KasI	GGCGCC	1 cut(s)	1948
Ksp22I	TGATCA	2 cut(s)	393, 2140
LmnI	GCTCC	4 cut(s)	494, 1430, 1613, 2098
Lsp1109I	GCAGC	6 cut(s)	259, 281, 468, 724, 1180, 2140
LweI	GCATC	4 cut(s)	1634, 1663, 1865, 2123
MaeI	CTAG	4 cut(s)	546, 764, 1605, 1809
MaeIII	GTNAC	6 cut(s)	99, 454, 665, 963, 1204, 1757
MboII	GAAGA	8 cut(s)	365, 832, 998, 1197, 1264, 1280, 1298, 1499
MfeI	CAATTG	1 cut(s)	23
MflI	RGATCY	4 cut(s)	520, 837, 1303, 1436
MhlI	GDGCHC	1 cut(s)	2024
Mly113I	GGCGCC	1 cut(s)	1949
MlyI	GAGTC	8 cut(s)	662, 814, 871, 1323, 1510, 1553, 1625, 1829
MmeI	TCCRAC	2 cut(s)	410, 1281
MroXI	GAANNNNTTC	2 cut(s)	903, 999
MseI	TTAA	6 cut(s)	75, 110, 225, 558, 1284, 2124
MslI	CAYNNNNRTG	3 cut(s)	226, 417, 1098
MspA1I	CMGCKG	1 cut(s)	272
MspI	CCGG	4 cut(s)	117, 705, 1611, 2150
MspR9I	CCNGG	4 cut(s)	551, 706, 1570, 2160
MunI	CAATTG	1 cut(s)	23
Mva1269I	GAATGC	1 cut(s)	271
MvaI	CCWGG	3 cut(s)	551, 1570, 2160
MvnI	CGCG	1 cut(s)	1546
MwoI	GCNNNNNNNGC	7 cut(s)	258, 278, 551, 710, 1177, 1340, 1622
NarI	GGCGCC	1 cut(s)	1949
NciI	CCSGG	1 cut(s)	706
NcoI	CCATGG	1 cut(s)	31
NheI	GCTAGC	1 cut(s)	545
NlaIII	CATG	8 cut(s)	35, 260, 757, 875, 953, 1135, 1427, 2075
NlaIV	GGNNCC	6 cut(s)	336, 522, 1716, 1950, 2094, 2148
NmuCI	GTSAC	3 cut(s)	454, 665, 963
NsbI	TGCGCA	1 cut(s)	1136
NspI	RCATGY	2 cut(s)	953, 1135
PaeR7I	CTCGAG	1 cut(s)	1837
PceI	AGGCCT	1 cut(s)	1978
PctI	GAATGC	1 cut(s)	271
PdmI	GAANNNNTTC	2 cut(s)	903, 999
PfeI	GAWTC	7 cut(s)	52, 152, 868, 904, 1409, 1601, 1829
Pfl23II	CGTACG	1 cut(s)	1505
PflMI	CCANNNNNTGG	2 cut(s)	37, 344
PkrI	GCNGC	7 cut(s)	271, 274, 483, 739, 1170, 1548, 2155
PleI	GAGTC	8 cut(s)	662, 814, 871, 1322, 1509, 1553, 1625, 1829
PluTI	GGCGCC	1 cut(s)	1952
PpsI	GAGTC	8 cut(s)	662, 814, 871, 1322, 1509, 1553, 1625, 1829
PpuMI	RGGWCCY	1 cut(s)	1714
PsiI	TTATAA	1 cut(s)	581
Psp5II	RGGWCCY	1 cut(s)	1714
Psp6I	CCWGG	3 cut(s)	549, 1568, 2158
PspEI	GGTNACC	1 cut(s)	963
PspGI	CCWGG	3 cut(s)	549, 1568, 2158
PspLI	CGTACG	1 cut(s)	1505
PspN4I	GGNNCC	6 cut(s)	336, 522, 1716, 1950, 2094, 2148
PspPI	GGNCC	3 cut(s)	189, 1333, 1714
PspPPI	RGGWCCY	1 cut(s)	1714
PstI	CTGCAG	2 cut(s)	727, 796
PstNI	CAGNNNCTG	1 cut(s)	1628
PsuI	RGATCY	4 cut(s)	520, 837, 1303, 1436
PvuII	CAGCTG	1 cut(s)	272
RsaI	GTAC	4 cut(s)	62, 813, 1357, 1507
RsaNI	GTAC	4 cut(s)	61, 812, 1356, 1506
RseI	CAYNNNNRTG	3 cut(s)	226, 417, 1098
SaqAI	TTAA	6 cut(s)	75, 110, 225, 558, 1284, 2124
SatI	GCNGC	7 cut(s)	270, 273, 482, 738, 1169, 1547, 2154
Sau96I	GGNCC	3 cut(s)	189, 1333, 1714
ScaI	AGTACT	1 cut(s)	813
SchI	GAGTC	8 cut(s)	662, 814, 871, 1323, 1510, 1553, 1625, 1829
ScrFI	CCNGG	4 cut(s)	551, 706, 1570, 2160
SduI	GDGCHC	1 cut(s)	2024
SfaNI	GCATC	4 cut(s)	1634, 1663, 1865, 2123
SfcI	CTRYAG	4 cut(s)	537, 723, 792, 1983
SfoI	GGCGCC	1 cut(s)	1950
Sfr274I	CTCGAG	1 cut(s)	1837
SinI	GGWCC	1 cut(s)	1714
SlaI	CTCGAG	1 cut(s)	1837
SmiMI	CAYNNNNRTG	3 cut(s)	226, 417, 1098
SmlI	CTYRAG	2 cut(s)	1158, 1837
SmoI	CTYRAG	2 cut(s)	1158, 1837
SseBI	AGGCCT	1 cut(s)	1978
SsiI	CCGC	1 cut(s)	1546
SspDI	GGCGCC	1 cut(s)	1948
SspI	AATATT	1 cut(s)	1120
SspMI	CTAG	4 cut(s)	546, 764, 1605, 1809
StuI	AGGCCT	1 cut(s)	1978
StyD4I	CCNGG	4 cut(s)	549, 704, 1568, 2158
StyI	CCWWGG	3 cut(s)	31, 1563, 1677
TaaI	ACNGT	9 cut(s)	202, 598, 811, 943, 1027, 1360, 1394, 1771, 1792
TaqI	TCGA	2 cut(s)	918, 1838
TatI	WGTACW	1 cut(s)	811
TauI	GCSGC	1 cut(s)	1549
TfiI	GAWTC	7 cut(s)	52, 152, 868, 904, 1409, 1601, 1829
Tru1I	TTAA	6 cut(s)	75, 110, 225, 558, 1284, 2124
Tru9I	TTAA	6 cut(s)	75, 110, 225, 558, 1284, 2124
TscAI	CASTG	6 cut(s)	1032, 1399, 1522, 1776, 1822, 1932
TseFI	GTSAC	3 cut(s)	454, 665, 963
TseI	GCWGC	6 cut(s)	269, 272, 481, 737, 1168, 2153
Tsp45I	GTSAC	3 cut(s)	454, 665, 963
TspDTI	ATGAA	7 cut(s)	6, 401, 742, 860, 912, 1281, 1441
TspGWI	ACGGA	1 cut(s)	352
TspRI	CASTG	6 cut(s)	1032, 1399, 1522, 1776, 1822, 1932
Van91I	CCANNNNNTGG	2 cut(s)	37, 344
VpaK11BI	GGWCC	1 cut(s)	1714
XapI	RAATTY	3 cut(s)	647, 1696, 1804
XbaI	TCTAGA	2 cut(s)	1604, 1808
XceI	RCATGY	2 cut(s)	953, 1135
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1093
XhoI	CTCGAG	1 cut(s)	1837
XmiI	GTMKAC	1 cut(s)	183
XmnI	GAANNNNTTC	2 cut(s)	903, 999
XspI	CTAG	4 cut(s)	546, 764, 1605, 1809
ZrmI	AGTACT	1 cut(s)	813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.