pycom15g27810

Cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012178
Physical Location & Seq
Reverse (-)
23315108 .. 23315653
546 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
ATGAGTTCTACTATTTTCTTGCTCTTTCTTCTCACAGTTGCATTTGCTCGCCAAAACCCTAATTTTCTCATCCAAGCTGACTCAACCCTGATTCAGAACGCATGCAAAAACACCAAGTACTATGGCCTTTGCGTCTCAAGCCTCAAATCTGTTCCCACAAGCATAAATGCAGACACAAAGGGGTTGGCAGTTATTATGGTTGGGATTGGAATGGCAAACGCAACTGCCACCTCATCGTACTTGTCCTCTCAGCTAATCAGCTCAAGCATCAATGACACAAACTTGAAGAAGGTCATCAAGGAGTGTGCCGACAAGTACGGCTACGTCGGTGATGCCCTTCAAGCTTCAGTGCAGGATTTAGGTTCAGAGTCTTATGATTATGCTTACATGCACATCACTGCAGCAGCTGACTACCCAAATGCCTGCCACAATGCTTTCAAAAGGTACCCAGGTTTGGCTTATCCTCCAGAACTTGCAAGAAGAGAGGAGGGATTGAAGCATATTTGTGATGTGGCTCTGGGAATTATTGATAATTTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0009628 GO:0030234 GO:0043086 GO:0044092 GO:0046910 GO:0050790 GO:0050896 GO:0065007 GO:0065009 GO:0080167 GO:0098772
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

182

Amino Acids

19.58

Weight (kDa)

6.26

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 30 - 174 1.8e-24 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64620
fragaria_vesca FvH4_1g24160
malus_domestica MD01G1038300.v1.1 MD15G1314600.v1.1
prunus_persica Prupe.6G197400_v2.0.a1
pyrus_communis pycom01g06630 pycom15g27810
rosa_chinensis RchiOBHm_Chr2g0120401
rosa_laevigata RLG00000018555
rosa_multiflora Rmu_co8012200.1_g000001 Rmu_sc0007380.1_g000001 Rmu_sc0007503.1_g000005
rosa_roxburghii Rroxscaffold_2G00123440
rosa_rugosa Rorug02G0229400
rosa_samantha Rh2AG285300 Rh2BG296400 Rh2CG274100 Rh2DG310300
rosa_wichuraiana Rw2G022900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 444
AccB1I GGYRCC 1 cut(s) 444
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 4 cut(s) 119, 239, 317, 446
AfiI CCNNNNNNNGG 1 cut(s) 454
AgsI TTSAA 4 cut(s) 286, 341, 439, 496
AjnI CCWGG 1 cut(s) 448
AluBI AGCT 5 cut(s) 77, 253, 261, 344, 407
AluI AGCT 5 cut(s) 77, 253, 261, 344, 407
Alw26I GTCTC 1 cut(s) 139
AlwNI CAGNNNCTG 1 cut(s) 407
AoxI GGCC 1 cut(s) 124
ApeKI GCWGC 2 cut(s) 401, 404
Asp718I GGTACC 1 cut(s) 444
AsuHPI GGTGA 1 cut(s) 341
BaeI ACNNNNGTAYC 2 cut(s) 436, 469
BanI GGYRCC 1 cut(s) 444
BbvI GCAGC 2 cut(s) 413, 416
BceAI ACGGC 1 cut(s) 334
BciT130I CCWGG 1 cut(s) 450
BcoDI GTCTC 1 cut(s) 139
BfmI CTRYAG 1 cut(s) 399
BisI GCNGC 2 cut(s) 402, 405
BlsI GCNGC 2 cut(s) 403, 406
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 1 cut(s) 450
BmiI GGNNCC 1 cut(s) 446
BmrFI CCNGG 1 cut(s) 450
BmsI GCATC 2 cut(s) 276, 322
BpmI CTGGAG 1 cut(s) 450
BpuEI CTTGAG 2 cut(s) 121, 247
BsaJI CCNNGG 1 cut(s) 448
Bsc4I CCNNNNNNNGG 1 cut(s) 454
BseBI CCWGG 1 cut(s) 450
BseDI CCNNGG 1 cut(s) 448
BseGI GGATG 1 cut(s) 69
BseLI CCNNNNNNNGG 1 cut(s) 454
BseMII CTCAG 1 cut(s) 263
BseRI GAGGAG 1 cut(s) 500
BseXI GCAGC 2 cut(s) 413, 416
BsgI GTGCAG 1 cut(s) 371
BshFI GGCC 1 cut(s) 126
BshNI GGYRCC 1 cut(s) 444
BslI CCNNNNNNNGG 1 cut(s) 454
BsmAI GTCTC 1 cut(s) 139
BsmBI CGTCTC 1 cut(s) 139
BsnI GGCC 1 cut(s) 126
BspANI GGCC 1 cut(s) 126
BspCNI CTCAG 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 446
BspMAI CTGCAG 1 cut(s) 403
BspT107I GGYRCC 1 cut(s) 444
BssECI CCNNGG 1 cut(s) 448
Bst2UI CCWGG 1 cut(s) 450
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 1 cut(s) 475
BstC8I GCNNGC 3 cut(s) 49, 103, 424
BstDEI CTNAG 1 cut(s) 249
BstF5I GGATG 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 139
BstMWI GCNNNNNNNGC 2 cut(s) 138, 341
BstNI CCWGG 1 cut(s) 450
BstNSI RCATGY 2 cut(s) 105, 391
BstSCI CCNGG 1 cut(s) 448
BstSFI CTRYAG 1 cut(s) 399
BstV1I GCAGC 2 cut(s) 413, 416
BsuRI GGCC 1 cut(s) 126
BtsCI GGATG 1 cut(s) 69
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 2 cut(s) 354, 396
Cac8I GCNNGC 3 cut(s) 49, 103, 424
CaiI CAGNNNCTG 1 cut(s) 407
CseI GACGC 1 cut(s) 121
Csp6I GTAC 4 cut(s) 118, 238, 316, 445
CviAII CATG 2 cut(s) 102, 388
CviQI GTAC 4 cut(s) 118, 238, 316, 445
DdeI CTNAG 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 475
EarI CTCTTC 1 cut(s) 475
Eco57I CTGAAG 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 448
Esp3I CGTCTC 1 cut(s) 139
FaeI CATG 2 cut(s) 105, 391
FaiI YATR 8 cut(s) 103, 123, 164, 197, 375, 381, 389, 501
FatI CATG 2 cut(s) 101, 387
Fnu4HI GCNGC 2 cut(s) 402, 405
FokI GGATG 1 cut(s) 56
Fsp4HI GCNGC 2 cut(s) 402, 405
GluI GCNGC 2 cut(s) 402, 405
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 1 cut(s) 126
HgaI GACGC 1 cut(s) 121
Hin1II CATG 2 cut(s) 105, 391
HindIII AAGCTT 1 cut(s) 342
HinfI GANTC 3 cut(s) 80, 91, 368
HphI GGTGA 1 cut(s) 341
Hpy188I TCNGA 2 cut(s) 96, 367
Hpy188III TCNNGA 1 cut(s) 467
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 2 cut(s) 283, 347
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 7 cut(s) 41, 105, 170, 352, 391, 401, 476
HpyF10VI GCNNNNNNNGC 2 cut(s) 138, 341
HpyF3I CTNAG 1 cut(s) 249
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 2 cut(s) 105, 391
KpnI GGTACC 1 cut(s) 448
LpnPI CCDG 7 cut(s) 101, 338, 435, 436, 462, 480, 503
Lsp1109I GCAGC 2 cut(s) 413, 416
LweI GCATC 2 cut(s) 276, 322
MaeII ACGT 1 cut(s) 324
MboII GAAGA 3 cut(s) 20, 298, 492
MluCI AATT 3 cut(s) 61, 522, 532
MlyI GAGTC 2 cut(s) 74, 377
MnlI CCTC 6 cut(s) 152, 241, 256, 474, 478, 481
MslI CAYNNNNRTG 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 407
MspR9I CCNGG 1 cut(s) 450
MvaI CCWGG 1 cut(s) 450
MwoI GCNNNNNNNGC 2 cut(s) 138, 341
NlaIII CATG 2 cut(s) 105, 391
NlaIV GGNNCC 1 cut(s) 446
NspI RCATGY 2 cut(s) 105, 391
PaeI GCATGC 1 cut(s) 105
PfeI GAWTC 1 cut(s) 91
PkrI GCNGC 2 cut(s) 403, 406
PleI GAGTC 2 cut(s) 74, 376
PpsI GAGTC 2 cut(s) 74, 376
Psp6I CCWGG 1 cut(s) 448
PspGI CCWGG 1 cut(s) 448
PspN4I GGNNCC 1 cut(s) 446
PstI CTGCAG 1 cut(s) 403
PstNI CAGNNNCTG 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 4 cut(s) 119, 239, 317, 446
RsaNI GTAC 4 cut(s) 118, 238, 316, 445
RseI CAYNNNNRTG 1 cut(s) 504
SatI GCNGC 2 cut(s) 402, 405
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 2 cut(s) 74, 377
ScrFI CCNGG 1 cut(s) 450
SfaNI GCATC 2 cut(s) 276, 322
SfcI CTRYAG 1 cut(s) 399
SmiMI CAYNNNNRTG 1 cut(s) 504
SmlI CTYRAG 2 cut(s) 136, 262
SmoI CTYRAG 2 cut(s) 136, 262
SphI GCATGC 1 cut(s) 105
Sse9I AATT 3 cut(s) 61, 522, 532
StyD4I CCNGG 1 cut(s) 448
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 1 cut(s) 327
TasI AATT 3 cut(s) 61, 522, 532
TatI WGTACW 1 cut(s) 117
TfiI GAWTC 1 cut(s) 91
TscAI CASTG 2 cut(s) 354, 403
TseI GCWGC 2 cut(s) 401, 404
TspRI CASTG 2 cut(s) 354, 403
XceI RCATGY 2 cut(s) 105, 391
ZrmI AGTACT 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.