pycom15g28150

Protein SCAI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009505
Physical Location & Seq
Forward (+)
23890158 .. 23895932
5775 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1818 bp
ATGTCCGAAAACGACGTCGTGACGCGGACGTTCCGGGCCCTGGTGGAGGGCGCCGACCGGAAGTTTGCTAGGGTTCGCGACGTGCCGGCGTACGGACGCCTCCACAACCAGCACTACTTCCACAAGGTCTTCAAGGCCTACATGCGCCTATGGAAGTACCAGCAGGAGAACCGCTCTAAGCTCGTCGCCGCCGGCCTGAACCGCTGGGAAATCGGCGAGATCGCCAGCCCGATCGGCCAGCTCTACTTCGGCCAGTACATGCGCACCAGCGAGGCCCGATTCCTCGTCGAGGCCTACGTCTTCTACGAGGCCATTCTCAGCCGCAGCTACTTCGACGGCTCCACCGGTTCCAGAGGTTTCGGGAAGAAGGATATCGGAGTCCGCTTCAAGGAGTTGAGATTCTACGCCCGGTTTTTGCTTGTTTCCTTGATTTTGAACCGAACCGAGACGGTTCGTCTTCTCGCCGAGCGGTTCAAATCGCTTGTTGATGACTGCAAAGCCAATTTTCGGGACACTAATTTTAAAGAATGGAGGCTAGTGGTGCAAGAAATTGCTCGATTTATGAAAGTTGATACGGCCTTTATGAATGTCAGGCCATTGCGATACTCTACCTTGTTTGATTCGCATCCAGCATCTCTTCCATATGTTGCTCGTTTCCATGCAAAGAGAGTCTTAAAATTTCAAGATGCATTGTTAACAAGCTATCACCATAATGAGGTCAAATTTGCTGAACTTACATTGGACACCTACCGAATGCTGCAATGTTTGGAATGGGAGCCAAGCGGGTCTTTCTACCAAAAGCGTCCAGTTGAACCAAAGGAGAATGGTGTTTTTATTGAACACTCTGGGGCTTCTGCAGCATCTGGAGTAATTGATATGAACTTGGCTGCGGACATGACTGATCCAAGTTTACCCCCAAATCCAAGGAAAGCTGTTATCTATCGTCCATCTGTGACACATTTGATAGCGGTGATGGCTACAATATGTGAGGAGCTTCCTCTGGACAGTATTATGCTAGTTTATCTGTCAGCCTCAGGGAAGGTTGGTCGCAGTAATGCTAGTCAGGTGTACAACTCTGGAGGATCACAGAAGTCCTCAAAAAACAAAGCTCCTCTCCAAGATCAGAATAAGTTTCTGCCTGAATCATGTATAAATAACAAGGGAGAGTCGAGTGGCTATTATGATCAATATCTGTGGTTTGGTCCTAGAGGAAATGGTGGTTTGAACAACCTTTACCCTGGCGATATAATTCCTTTTACTCGTAGGCCTCTTTTCTTGGTAATAGATAGCGACAACAGCCATGCATTCAAGGTTTTACATGGAGCGGAAAGAGGAGAGACGGCTGCTCTGCTTCTTTCACCTTTGAGACCAGCGTTCAAGAATCCAGCTGATGCTGATTTAACGCAAAATGGAAGTCAGTTTACCTTTTTCTTGACTGCTCCCTTGGCAGCATTCTGCCAATTAGTTGGATTCTCCTCTTCTGATACCGAAGCAGAGGTTTATAGCAAAGCTGAAAGCATACTCTTGGCTGCCTTCTCTGAGTGGGAAGTTATTCTTTGTACGACAAGAAGCCTGGATCTGGTATGGGCACAAGTTCTATCAGATCCTTTTTTGCGGCGACTTATTCTGAGATTTATATTCTGCAGAGCTGTGCTTTCTTTCTTCTGCCCTCCTGAAGATAGTGAGCAGCATTTGCCGGTTTGCCTACCTATTCTTCCCAATCCCGTTTCTCCAGATTCTGAAGTTGTGTGGTCTGCTGTCCACAGGCTTGCAAAGCACCTCACCGTCTCTGACTATTTTCACTTTGTTGACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAI

Protein Analysis

606

Amino Acids

68.73

Weight (kDa)

8.65

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAI PF12070 14 - 556 8.1e-200 Protein SCAI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 18
Acc16I TGCGCA 1 cut(s) 263
AccB1I GGYRCC 1 cut(s) 50
AccBSI CCGCTC 3 cut(s) 174, 469, 1325
AccII CGCG 2 cut(s) 25, 78
AclWI GGATC 4 cut(s) 896, 1090, 1584, 1598
AcoI YGGCCR 2 cut(s) 235, 250
AcsI RAATTY 2 cut(s) 677, 722
AcuI CTGAAG 2 cut(s) 1695, 1761
AcyI GRCGYC 3 cut(s) 15, 51, 97
AfaI GTAC 5 cut(s) 92, 158, 257, 1070, 1561
AfiI CCNNNNNNNGG 8 cut(s) 40, 46, 92, 289, 388, 507, 715, 1579
AgeI ACCGGT 1 cut(s) 344
AhdI GACNNNNNGTC 1 cut(s) 453
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 3 cut(s) 39, 1237, 1572
Alw26I GTCTC 4 cut(s) 440, 1331, 1360, 1792
AlwI GGATC 4 cut(s) 896, 1090, 1584, 1598
AlwNI CAGNNNCTG 2 cut(s) 863, 1739
ApaI GGGCCC 1 cut(s) 40
ApeKI GCWGC 8 cut(s) 324, 757, 857, 887, 1343, 1448, 1529, 1687
ApoI RAATTY 2 cut(s) 677, 722
ArsI GACNNNNNNTTYG 5 cut(s) 32, 47, 79, 1399, 1431
AsiGI ACCGGT 1 cut(s) 344
Asp700I GAANNNNTTC 1 cut(s) 1551
AspLEI GCGC 3 cut(s) 53, 147, 264
AspS9I GGNCC 4 cut(s) 36, 37, 274, 1202
AsuC2I CCSGG 2 cut(s) 35, 409
AsuHPI GGTGA 4 cut(s) 698, 982, 1350, 1774
AvaII GGWCC 1 cut(s) 1202
AxyI CCTNAGG 1 cut(s) 1033
BaeGI GKGCMC 2 cut(s) 40, 1591
BanI GGYRCC 1 cut(s) 50
BanII GRGCYC 1 cut(s) 40
BarI GAAGNNNNNNTAC 2 cut(s) 1405, 1437
BbsI GAAGAC 3 cut(s) 121, 292, 449
BbvI GCAGC 8 cut(s) 336, 744, 869, 874, 1330, 1460, 1516, 1699
BccI CCATC 2 cut(s) 955, 967
BceAI ACGGC 3 cut(s) 352, 591, 1356
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 3 cut(s) 41, 1239, 1574
BclI TGATCA 1 cut(s) 1183
BcnI CCSGG 2 cut(s) 35, 409
BcoDI GTCTC 4 cut(s) 440, 1331, 1360, 1792
BfaI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
BfmI CTRYAG 2 cut(s) 855, 1642
BfoI RGCGCY 1 cut(s) 54
BglI GCCNNNNNGGC 1 cut(s) 234
Bme1390I CCNGG 5 cut(s) 35, 41, 409, 1239, 1574
Bme18I GGWCC 1 cut(s) 1202
BmeRI GACNNNNNGTC 1 cut(s) 453
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 4 cut(s) 36, 37, 274, 1202
BmiI GGNNCC 5 cut(s) 38, 52, 340, 349, 777
BmrFI CCNGG 5 cut(s) 35, 41, 409, 1239, 1574
BmsI GCATC 5 cut(s) 634, 641, 676, 869, 1381
BpiI GAAGAC 3 cut(s) 121, 292, 449
BplI GAGNNNNNCTC 2 cut(s) 158, 190
BpmI CTGGAG 3 cut(s) 885, 1098, 1716
BpuMI CCSGG 2 cut(s) 35, 409
BsaBI GATNNNNATC 2 cut(s) 624, 1188
BsaHI GRCGYC 3 cut(s) 15, 51, 97
BsaI GGTCTC 1 cut(s) 1360
BsaJI CCNNGG 4 cut(s) 39, 923, 1237, 1443
BsaWI WCCGGW 2 cut(s) 57, 344
Bsc4I CCNNNNNNNGG 8 cut(s) 40, 46, 92, 289, 388, 507, 715, 1579
Bse118I RCCGGY 4 cut(s) 85, 191, 344, 1696
Bse1I ACTGG 2 cut(s) 253, 806
Bse21I CCTNAGG 1 cut(s) 1033
Bse3DI GCAATG 2 cut(s) 596, 767
Bse8I GATNNNNATC 2 cut(s) 624, 1188
BseBI CCWGG 3 cut(s) 41, 1239, 1574
BseDI CCNNGG 4 cut(s) 39, 923, 1237, 1443
BseGI GGATG 1 cut(s) 625
BseJI GATNNNNATC 2 cut(s) 624, 1188
BseLI CCNNNNNNNGG 8 cut(s) 40, 46, 92, 289, 388, 507, 715, 1579
BseMI GCAATG 2 cut(s) 596, 767
BseMII CTCAG 4 cut(s) 331, 1047, 1530, 1619
BseNI ACTGG 2 cut(s) 253, 806
BseRI GAGGAG 4 cut(s) 1004, 1101, 1347, 1465
BseSI GKGCMC 2 cut(s) 40, 1591
BseXI GCAGC 8 cut(s) 336, 744, 869, 874, 1330, 1460, 1516, 1699
BseYI CCCAGC 1 cut(s) 204
Bsh1236I CGCG 2 cut(s) 25, 78
Bsh1285I CGRYCG 2 cut(s) 58, 234
BshNI GGYRCC 1 cut(s) 50
BshTI ACCGGT 1 cut(s) 344
BsiEI CGRYCG 2 cut(s) 58, 234
BsiSI CCGG 7 cut(s) 34, 58, 86, 192, 345, 409, 1697
BsiWI CGTACG 1 cut(s) 90
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 8 cut(s) 40, 46, 92, 289, 388, 507, 715, 1579
BsmAI GTCTC 4 cut(s) 440, 1331, 1360, 1792
BsmBI CGTCTC 3 cut(s) 440, 1331, 1792
BsmFI GGGAC 1 cut(s) 524
BsmI GAATGC 3 cut(s) 759, 1304, 1451
Bso31I GGTCTC 1 cut(s) 1360
Bsp120I GGGCCC 1 cut(s) 36
Bsp1286I GDGCHC 2 cut(s) 40, 1591
Bsp1407I TGTACA 1 cut(s) 1068
Bsp143I GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
Bsp68I TCGCGA 1 cut(s) 78
BspCNI CTCAG 4 cut(s) 330, 1046, 1531, 1620
BspFNI CGCG 2 cut(s) 25, 78
BspLI GGNNCC 5 cut(s) 38, 52, 340, 349, 777
BspMAI CTGCAG 2 cut(s) 859, 1646
BspPI GGATC 4 cut(s) 896, 1090, 1584, 1598
BspT107I GGYRCC 1 cut(s) 50
BspTNI GGTCTC 1 cut(s) 1360
BsrBI CCGCTC 3 cut(s) 174, 469, 1325
BsrDI GCAATG 2 cut(s) 596, 767
BsrFI RCCGGY 4 cut(s) 85, 191, 344, 1696
BsrGI TGTACA 1 cut(s) 1068
BsrI ACTGG 2 cut(s) 253, 806
BssAI RCCGGY 4 cut(s) 85, 191, 344, 1696
BssECI CCNNGG 4 cut(s) 39, 923, 1237, 1443
BssMI GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
BssNI GRCGYC 3 cut(s) 15, 51, 97
BssT1I CCWWGG 2 cut(s) 923, 1443
Bst2UI CCWGG 3 cut(s) 41, 1239, 1574
Bst4CI ACNGT 3 cut(s) 451, 1007, 1786
Bst6I CTCTTC 2 cut(s) 642, 1483
BstACI GRCGYC 3 cut(s) 15, 51, 97
BstAPI GCANNNNNTGC 1 cut(s) 1693
BstAUI TGTACA 1 cut(s) 1068
BstC8I GCNNGC 5 cut(s) 87, 193, 226, 239, 1770
BstDEI CTNAG 5 cut(s) 177, 317, 1033, 1539, 1628
BstENI CCTNNNNNAGG 2 cut(s) 44, 287
BstF5I GGATG 1 cut(s) 625
BstFNI CGCG 2 cut(s) 25, 78
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 3 cut(s) 53, 147, 264
BstKTI GATC 8 cut(s) 222, 234, 904, 1085, 1123, 1186, 1579, 1606
BstMAI GTCTC 4 cut(s) 440, 1331, 1360, 1792
BstMBI GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
BstMCI CGRYCG 2 cut(s) 58, 234
BstMWI GCNNNNNNNGC 9 cut(s) 201, 234, 541, 857, 974, 1296, 1445, 1693, 1774
BstNI CCWGG 3 cut(s) 41, 1239, 1574
BstNSI RCATGY 2 cut(s) 145, 262
BstSCI CCNGG 5 cut(s) 33, 39, 407, 1237, 1572
BstSFI CTRYAG 2 cut(s) 855, 1642
BstSLI GKGCMC 2 cut(s) 40, 1591
BstUI CGCG 2 cut(s) 25, 78
BstV1I GCAGC 8 cut(s) 336, 744, 869, 874, 1330, 1460, 1516, 1699
BstV2I GAAGAC 3 cut(s) 121, 292, 449
BstX2I RGATCY 2 cut(s) 1576, 1603
BstXI CCANNNNNNTGG 1 cut(s) 1466
BstYI RGATCY 2 cut(s) 1576, 1603
Bsu36I CCTNAGG 1 cut(s) 1033
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 1 cut(s) 625
BtuMI TCGCGA 1 cut(s) 78
Cac8I GCNNGC 5 cut(s) 87, 193, 226, 239, 1770
CaiI CAGNNNCTG 2 cut(s) 863, 1739
CfoI GCGC 3 cut(s) 53, 147, 264
Cfr10I RCCGGY 4 cut(s) 85, 191, 344, 1696
Cfr13I GGNCC 4 cut(s) 36, 37, 274, 1202
CseI GACGC 3 cut(s) 31, 105, 791
Csp6I GTAC 5 cut(s) 91, 157, 256, 1069, 1560
CspAI ACCGGT 1 cut(s) 344
CspCI CAANNNNNGTGG 2 cut(s) 1175, 1210
CviAII CATG 8 cut(s) 142, 259, 659, 895, 1146, 1301, 1319, 1815
CviQI GTAC 5 cut(s) 91, 157, 256, 1069, 1560
DdeI CTNAG 5 cut(s) 177, 317, 1033, 1539, 1628
DinI GGCGCC 1 cut(s) 52
DpnI GATC 8 cut(s) 221, 233, 903, 1084, 1122, 1185, 1578, 1605
DpnII GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
DraI TTTAAA 1 cut(s) 523
DriI GACNNNNNGTC 1 cut(s) 453
EaeI YGGCCR 2 cut(s) 235, 250
Eam1104I CTCTTC 2 cut(s) 642, 1483
Eam1105I GACNNNNNGTC 1 cut(s) 453
EarI CTCTTC 2 cut(s) 642, 1483
Eco130I CCWWGG 2 cut(s) 923, 1443
Eco147I AGGCCT 3 cut(s) 137, 293, 1267
Eco24I GRGCYC 1 cut(s) 40
Eco31I GGTCTC 1 cut(s) 1360
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 1 cut(s) 1202
Eco57I CTGAAG 2 cut(s) 1695, 1761
Eco81I CCTNAGG 1 cut(s) 1033
EcoNI CCTNNNNNAGG 2 cut(s) 44, 287
EcoO109I RGGNCCY 1 cut(s) 37
EcoRII CCWGG 3 cut(s) 39, 1237, 1572
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 2 cut(s) 923, 1443
EcoT22I ATGCAT 2 cut(s) 691, 1306
EcoT38I GRGCYC 1 cut(s) 40
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
ErhI CCWWGG 2 cut(s) 923, 1443
Esp3I CGTCTC 3 cut(s) 440, 1331, 1792
FaeI CATG 8 cut(s) 145, 262, 662, 898, 1149, 1304, 1322, 1818
FalI AAGNNNNNCTT 6 cut(s) 656, 688, 772, 804, 1539, 1571
FaqI GGGAC 1 cut(s) 524
FatI CATG 8 cut(s) 141, 258, 658, 894, 1145, 1300, 1318, 1814
FauI CCCGC 1 cut(s) 776
FauNDI CATATG 1 cut(s) 643
FbaI TGATCA 1 cut(s) 1183
FokI GGATG 1 cut(s) 612
FriOI GRGCYC 1 cut(s) 40
FspAI RTGCGCAY 1 cut(s) 263
FspBI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
FspI TGCGCA 1 cut(s) 263
GlaI GCGC 3 cut(s) 52, 146, 263
GsaI CCCAGC 1 cut(s) 208
GsuI CTGGAG 3 cut(s) 885, 1098, 1716
HaeII RGCGCY 1 cut(s) 54
HapII CCGG 7 cut(s) 34, 58, 86, 192, 345, 409, 1697
HgaI GACGC 3 cut(s) 31, 105, 791
HhaI GCGC 3 cut(s) 53, 147, 264
Hin1I GRCGYC 3 cut(s) 15, 51, 97
Hin1II CATG 8 cut(s) 145, 262, 662, 898, 1149, 1304, 1322, 1818
Hin6I GCGC 3 cut(s) 51, 145, 262
HinP1I GCGC 3 cut(s) 51, 145, 262
HincII GTYRAC 2 cut(s) 696, 1810
HindII GTYRAC 2 cut(s) 696, 1810
HpaI GTTAAC 1 cut(s) 696
HpaII CCGG 7 cut(s) 34, 58, 86, 192, 345, 409, 1697
HphI GGTGA 4 cut(s) 698, 982, 1350, 1774
Hpy166II GTNNAC 6 cut(s) 696, 911, 1069, 1422, 1762, 1810
Hpy188I TCNGA 9 cut(s) 7, 377, 1125, 1483, 1540, 1603, 1629, 1741, 1792
Hpy8I GTNNAC 6 cut(s) 696, 911, 1069, 1422, 1762, 1810
Hpy99I CGWCG 6 cut(s) 17, 20, 83, 188, 290, 338
HpyAV CCTTC 3 cut(s) 361, 1033, 1543
HpyCH4III ACNGT 3 cut(s) 451, 1007, 1786
HpyCH4IV ACGT 4 cut(s) 15, 29, 81, 297
HpyCH4V TGCA 9 cut(s) 495, 544, 662, 689, 760, 857, 1304, 1644, 1772
HpyF10VI GCNNNNNNNGC 9 cut(s) 201, 234, 541, 857, 974, 1296, 1445, 1693, 1774
HpyF3I CTNAG 5 cut(s) 177, 317, 1033, 1539, 1628
HpySE526I ACGT 4 cut(s) 15, 29, 81, 297
Hsp92I GRCGYC 3 cut(s) 15, 51, 97
Hsp92II CATG 8 cut(s) 145, 262, 662, 898, 1149, 1304, 1322, 1818
HspAI GCGC 3 cut(s) 51, 145, 262
KasI GGCGCC 1 cut(s) 50
KroI GCCGGC 2 cut(s) 85, 191
KroNI GCCGGC 2 cut(s) 87, 193
Ksp22I TGATCA 1 cut(s) 1183
KspAI GTTAAC 1 cut(s) 696
Kzo9I GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
LmnI GCTCC 6 cut(s) 344, 775, 991, 1114, 1322, 1444
Lsp1109I GCAGC 8 cut(s) 336, 744, 869, 874, 1330, 1460, 1516, 1699
LweI GCATC 5 cut(s) 634, 641, 676, 869, 1381
MaeI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
MaeII ACGT 4 cut(s) 15, 29, 81, 297
MaeIII GTNAC 2 cut(s) 19, 952
MalI GATC 8 cut(s) 221, 233, 903, 1084, 1122, 1185, 1578, 1605
MbiI CCGCTC 3 cut(s) 174, 469, 1325
MboI GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
MboII GAAGA 9 cut(s) 121, 292, 376, 449, 629, 1470, 1654, 1688, 1706
MflI RGATCY 2 cut(s) 1576, 1603
MhlI GDGCHC 2 cut(s) 40, 1591
MluCI AATT 8 cut(s) 502, 517, 549, 677, 722, 870, 1248, 1460
Mly113I GGCGCC 1 cut(s) 51
MlyI GAGTC 3 cut(s) 387, 678, 1175
MmeI TCCRAC 1 cut(s) 1447
Mph1103I ATGCAT 2 cut(s) 691, 1306
MroNI GCCGGC 2 cut(s) 85, 191
MroXI GAANNNNTTC 1 cut(s) 1551
MseI TTAA 4 cut(s) 522, 674, 695, 1400
MslI CAYNNNNRTG 1 cut(s) 711
MspA1I CMGCKG 2 cut(s) 204, 1388
MspI CCGG 7 cut(s) 34, 58, 86, 192, 345, 409, 1697
MspR9I CCNGG 5 cut(s) 35, 41, 409, 1239, 1574
Mva1269I GAATGC 3 cut(s) 759, 1304, 1451
MvaI CCWGG 3 cut(s) 41, 1239, 1574
MvnI CGCG 2 cut(s) 25, 78
MwoI GCNNNNNNNGC 9 cut(s) 201, 234, 541, 857, 974, 1296, 1445, 1693, 1774
NaeI GCCGGC 2 cut(s) 87, 193
NarI GGCGCC 1 cut(s) 51
NciI CCSGG 2 cut(s) 35, 409
NdeI CATATG 1 cut(s) 643
NdeII GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
NgoMIV GCCGGC 2 cut(s) 85, 191
NlaIII CATG 8 cut(s) 145, 262, 662, 898, 1149, 1304, 1322, 1818
NlaIV GGNNCC 5 cut(s) 38, 52, 340, 349, 777
NmeAIII GCCGAG 1 cut(s) 490
NmuCI GTSAC 2 cut(s) 19, 952
NruI TCGCGA 1 cut(s) 78
NsbI TGCGCA 1 cut(s) 263
NsiI ATGCAT 2 cut(s) 691, 1306
NspI RCATGY 2 cut(s) 145, 262
PceI AGGCCT 3 cut(s) 137, 293, 1267
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 303
PctI GAATGC 3 cut(s) 759, 1304, 1451
PdiI GCCGGC 2 cut(s) 87, 193
PdmI GAANNNNTTC 1 cut(s) 1551
PfeI GAWTC 7 cut(s) 279, 399, 620, 1142, 1381, 1470, 1736
Pfl23II CGTACG 1 cut(s) 90
PinAI ACCGGT 1 cut(s) 344
Ple19I CGATCG 1 cut(s) 234
PleI GAGTC 3 cut(s) 386, 677, 1174
PluTI GGCGCC 1 cut(s) 54
PpsI GAGTC 3 cut(s) 386, 677, 1174
Psp6I CCWGG 3 cut(s) 39, 1237, 1572
PspFI CCCAGC 1 cut(s) 204
PspGI CCWGG 3 cut(s) 39, 1237, 1572
PspLI CGTACG 1 cut(s) 90
PspN4I GGNNCC 5 cut(s) 38, 52, 340, 349, 777
PspOMI GGGCCC 1 cut(s) 36
PspPI GGNCC 4 cut(s) 36, 37, 274, 1202
PsrI GAACNNNNNNTAC 2 cut(s) 1217, 1249
PstI CTGCAG 2 cut(s) 859, 1646
PstNI CAGNNNCTG 2 cut(s) 863, 1739
PsuI RGATCY 2 cut(s) 1576, 1603
PvuI CGATCG 1 cut(s) 234
PvuII CAGCTG 1 cut(s) 1388
RruI TCGCGA 1 cut(s) 78
RsaI GTAC 5 cut(s) 92, 158, 257, 1070, 1561
RsaNI GTAC 5 cut(s) 91, 157, 256, 1069, 1560
RseI CAYNNNNRTG 1 cut(s) 711
SaqAI TTAA 4 cut(s) 522, 674, 695, 1400
Sau3AI GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
Sau96I GGNCC 4 cut(s) 36, 37, 274, 1202
SchI GAGTC 3 cut(s) 387, 678, 1175
ScrFI CCNGG 5 cut(s) 35, 41, 409, 1239, 1574
SduI GDGCHC 2 cut(s) 40, 1591
SfaNI GCATC 5 cut(s) 634, 641, 676, 869, 1381
SfcI CTRYAG 2 cut(s) 855, 1642
SfoI GGCGCC 1 cut(s) 52
SinI GGWCC 1 cut(s) 1202
SmiMI CAYNNNNRTG 1 cut(s) 711
Sse9I AATT 8 cut(s) 502, 517, 549, 677, 722, 870, 1248, 1460
SseBI AGGCCT 3 cut(s) 137, 293, 1267
SspDI GGCGCC 1 cut(s) 50
SspMI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
StuI AGGCCT 3 cut(s) 137, 293, 1267
StyD4I CCNGG 5 cut(s) 33, 39, 407, 1237, 1572
StyI CCWWGG 2 cut(s) 923, 1443
TaaI ACNGT 3 cut(s) 451, 1007, 1786
TaiI ACGT 4 cut(s) 18, 32, 84, 300
TaqI TCGA 4 cut(s) 288, 333, 556, 1169
TasI AATT 8 cut(s) 502, 517, 549, 677, 722, 870, 1248, 1460
TatI WGTACW 2 cut(s) 255, 1068
TauI GCSGC 3 cut(s) 191, 324, 1618
TfiI GAWTC 7 cut(s) 279, 399, 620, 1142, 1381, 1470, 1736
Tru1I TTAA 4 cut(s) 522, 674, 695, 1400
Tru9I TTAA 4 cut(s) 522, 674, 695, 1400
TseFI GTSAC 2 cut(s) 19, 952
TseI GCWGC 8 cut(s) 324, 757, 857, 887, 1343, 1448, 1529, 1687
Tsp45I GTSAC 2 cut(s) 19, 952
TspDTI ATGAA 3 cut(s) 578, 599, 893
TspGWI ACGGA 1 cut(s) 108
VpaK11BI GGWCC 1 cut(s) 1202
XagI CCTNNNNNAGG 2 cut(s) 44, 287
XapI RAATTY 2 cut(s) 677, 722
XceI RCATGY 2 cut(s) 145, 262
XmnI GAANNNNTTC 1 cut(s) 1551
XspI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
ZraI GACGTC 1 cut(s) 16
Zsp2I ATGCAT 2 cut(s) 691, 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.