pycom15g28950

TSL-kinase interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007256
Physical Location & Seq
Forward (+)
25463006 .. 25466525
3520 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1491 bp
ATGAAAGTTTGTAGAGAAACAAACAGGAAGGTGATCAAGGTTCCTATAGAAAGCAGAGCAGGTATAGGAAGTAGCATGAGAGAACAGAAAACAGGGAGAACTACCCGAGTCTGCCCCAAAGCAACAGGGAAAGGTGGGCGTGCTGATGGCATGTATTTGGAGCTTCCTGACAAAGGTGGGCCCTTTTCAGGAATAACAAAGAGACCGCCTGATCTTGAGTGTTGTCAAGGGAAAACACTTCTTCCACGGTCAAAGATCAAGTTGCAGCTTTTCCCTGTAAATGAAGTTACTCGCATACGATTGGAAAAGGATGGACATCATCCATATTTAGAACTCATTCTTAGACCACAGAAGAAGATTACTTCTGTGCTAAAGCACCTTAATGACAAGTGGGGGAGTTCAAGCGTTGCTCTTAATGAGCTGGTTCTATTCCCATATAAGATGCATGACAGTATGTCTAGTAACAGAAGATGGACACTGAATGATGGTGACATTAGTGCCGGAGCTGTCTATGCAGATATTGGATGCCCTGCAGTTTTTCGCTTAAGGTATGGCTGGCTCTCTACTGAACCTAAAGGTGCTGGTCAACCTTCTACATCAGCTAATGACTTACAATCTGAGGTTGAACAGAGATGTAGAAGCGCCTCCACAGAGAATGTATATGATGAATTAGGGAAACAGACTGTGTTAGCAACAAAAGATTTCAGATCAATCAAAGCAAGAGAAACAATAAATGCAGATGCTGAGAATATTTCTACTGGAGTTGTTAATCTCAAGGACGATATTCAGAGGAGGATGGGTAACCATGCTCAGGAACCACTTTTGCTTGACAGTGTAACTAATATAAGCATTGGAGGCCTACTTTCTGAAGCATCTTTACAGGCCAAGTGCAATAACAGTGAAGAAAAACAAATTGGGAAAAATGAAGGCTTTCATCCTACTGAAACAAAAAATTCAAAGTCATTTGCGTTGTGGGATTGTCCTTCTATAAGCATTGGTGGCCTCTTGTCCGAAGCATCCATGCAGGGCAAGCTCGATCAATTTTATGCACAACCAGTTGAGAGCAATGCAGGCTTGCCACATCCTCAAGGTCAAAGGCCATCCACTCATGACTCATGCTTATCTATTTTTGATGCTGAAGAAACTTGCCATGCATTTCCTTCTCAAAAATTCTCTTTGTCGGGAAAAGATGTTCTGGGGTTTGGTGGAAGTCGTGGAGGTTTCAACCAGAATCCTGGTTCTAAACTATTCAAGCTCCCTAATGCAGCAAAGTCTAAAAATGTAGATGGGCTGCTGCAAGACCAGCGTGCTTGTCAGGAATCGAATACAGCTCTCATGATTTGTTCGCGAGTGAACAACGATGACAAAAGTCTTGGGCTGTCAGGCATCAAATGGACCGACTCCTTGGGGCCGTTTGATCTTGGCCTCCCTGTTTCCCAGAAGCTCATCACTGGAGAGAGTACAAGCAGCATCAGTGGATTTGTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
No domains found.

Protein Analysis

497

Amino Acids

54.09

Weight (kDa)

8.85

Isoelectric Point (pI)

49.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 50
AccII CGCG 1 cut(s) 1348
AciI CCGC 1 cut(s) 206
AcsI RAATTY 2 cut(s) 952, 1169
AcuI CTGAAG 2 cut(s) 888, 1158
AfaI GTAC 1 cut(s) 1462
AfiI CCNNNNNNNGG 3 cut(s) 173, 188, 811
AflII CTTAAG 1 cut(s) 544
AgsI TTSAA 5 cut(s) 402, 626, 957, 1225, 1252
AhdI GACNNNNNGTC 1 cut(s) 454
AjnI CCWGG 1 cut(s) 1234
AjuI GAANNNNNNNTTGG 2 cut(s) 897, 929
AloI GAACNNNNNNTCC 2 cut(s) 1176, 1208
AluBI AGCT 9 cut(s) 163, 268, 421, 506, 602, 1033, 1255, 1331, 1444
AluI AGCT 9 cut(s) 163, 268, 421, 506, 602, 1033, 1255, 1331, 1444
Alw26I GTCTC 1 cut(s) 196
AlwNI CAGNNNCTG 1 cut(s) 743
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 7 cut(s) 179, 856, 882, 1000, 1097, 1409, 1423
ApaI GGGCCC 1 cut(s) 183
ApeKI GCWGC 5 cut(s) 265, 1265, 1291, 1294, 1467
ApoI RAATTY 2 cut(s) 952, 1169
Asp700I GAANNNNTTC 2 cut(s) 336, 930
AspLEI GCGC 1 cut(s) 644
AspS9I GGNCC 4 cut(s) 179, 180, 1395, 1409
AsuHPI GGTGA 2 cut(s) 43, 500
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 1 cut(s) 1395
BaeGI GKGCMC 1 cut(s) 183
BanII GRGCYC 1 cut(s) 183
BarI GAAGNNNNNNTAC 2 cut(s) 861, 893
BbvI GCAGC 5 cut(s) 277, 1277, 1278, 1281, 1479
BccI CCATC 7 cut(s) 140, 305, 465, 479, 790, 1108, 1280
BceAI ACGGC 1 cut(s) 1396
BciT130I CCWGG 1 cut(s) 1236
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 196
BfaI CTAG 1 cut(s) 459
BfmI CTRYAG 2 cut(s) 45, 531
BfoI RGCGCY 1 cut(s) 645
BfrI CTTAAG 1 cut(s) 544
BfuAI ACCTGC 1 cut(s) 50
BisI GCNGC 5 cut(s) 266, 1266, 1292, 1295, 1468
BlsI GCNGC 5 cut(s) 267, 1267, 1293, 1296, 1469
Bme1390I CCNGG 1 cut(s) 1236
Bme18I GGWCC 1 cut(s) 1395
BmeRI GACNNNNNGTC 1 cut(s) 454
BmeT110I CYCGRG 1 cut(s) 105
BmgT120I GGNCC 4 cut(s) 179, 180, 1395, 1409
BmiI GGNNCC 4 cut(s) 42, 181, 816, 1410
BmrFI CCNGG 1 cut(s) 1236
BmsI GCATC 8 cut(s) 432, 515, 730, 881, 1025, 1123, 1395, 1479
BoxI GACNNNNGTC 1 cut(s) 1368
BpmI CTGGAG 2 cut(s) 780, 1473
Bpu10I CCTNAGC 1 cut(s) 810
BpuEI CTTGAG 3 cut(s) 236, 758, 1071
BsaBI GATNNNNATC 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 196
BsaJI CCNNGG 2 cut(s) 245, 1404
BsaXI ACNNNNNCTCC 2 cut(s) 784, 814
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 188, 811
Bse1I ACTGG 3 cut(s) 763, 1055, 1456
Bse3DI GCAATG 1 cut(s) 1072
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 1 cut(s) 1236
BseDI CCNNGG 2 cut(s) 245, 1404
BseGI GGATG 8 cut(s) 316, 319, 530, 801, 934, 1016, 1081, 1100
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 3 cut(s) 173, 188, 811
BseMI GCAATG 1 cut(s) 1072
BseMII CTCAG 3 cut(s) 609, 735, 824
BseNI ACTGG 3 cut(s) 763, 1055, 1456
BseRI GAGGAG 1 cut(s) 805
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 5 cut(s) 277, 1277, 1278, 1281, 1479
Bsh1236I CGCG 1 cut(s) 1348
BshFI GGCC 7 cut(s) 181, 858, 884, 1002, 1099, 1411, 1425
BsiHKCI CYCGRG 1 cut(s) 105
BsiSI CCGG 1 cut(s) 501
BslI CCNNNNNNNGG 3 cut(s) 173, 188, 811
BsmAI GTCTC 1 cut(s) 196
BsnI GGCC 7 cut(s) 181, 858, 884, 1002, 1099, 1411, 1425
Bso31I GGTCTC 1 cut(s) 196
BsoBI CYCGRG 1 cut(s) 105
Bsp120I GGGCCC 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 6 cut(s) 33, 211, 255, 707, 1036, 1417
Bsp68I TCGCGA 1 cut(s) 1348
BspACI CCGC 1 cut(s) 206
BspANI GGCC 7 cut(s) 181, 858, 884, 1002, 1099, 1411, 1425
BspCNI CTCAG 3 cut(s) 610, 736, 823
BspFNI CGCG 1 cut(s) 1348
BspHI TCATGA 2 cut(s) 1108, 1335
BspLI GGNNCC 4 cut(s) 42, 181, 816, 1410
BspMAI CTGCAG 1 cut(s) 535
BspMI ACCTGC 1 cut(s) 50
BspTI CTTAAG 1 cut(s) 544
BspTNI GGTCTC 1 cut(s) 196
BsrDI GCAATG 1 cut(s) 1072
BsrI ACTGG 3 cut(s) 763, 1055, 1456
BssECI CCNNGG 2 cut(s) 245, 1404
BssMI GATC 6 cut(s) 33, 211, 255, 707, 1036, 1417
BssT1I CCWWGG 1 cut(s) 1404
Bst2UI CCWGG 1 cut(s) 1236
Bst4CI ACNGT 5 cut(s) 249, 452, 685, 833, 899
BstAFI CTTAAG 1 cut(s) 544
BstC8I GCNNGC 6 cut(s) 141, 557, 1031, 1072, 1076, 1308
BstDEI CTNAG 4 cut(s) 341, 618, 744, 810
BstDSI CCRYGG 1 cut(s) 245
BstEII GGTNACC 1 cut(s) 800
BstENI CCTNNNNNAGG 1 cut(s) 171
BstF5I GGATG 8 cut(s) 316, 319, 530, 801, 934, 1016, 1081, 1100
BstFNI CGCG 1 cut(s) 1348
BstH2I RGCGCY 1 cut(s) 645
BstHHI GCGC 1 cut(s) 644
BstKTI GATC 6 cut(s) 36, 214, 258, 710, 1039, 1420
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 6 cut(s) 33, 211, 255, 707, 1036, 1417
BstMWI GCNNNNNNNGC 6 cut(s) 512, 855, 999, 1030, 1071, 1303
BstNI CCWGG 1 cut(s) 1236
BstNSI RCATGY 1 cut(s) 154
BstPAI GACNNNNGTC 1 cut(s) 1368
BstPI GGTNACC 1 cut(s) 800
BstSCI CCNGG 1 cut(s) 1234
BstSFI CTRYAG 2 cut(s) 45, 531
BstSLI GKGCMC 1 cut(s) 183
BstUI CGCG 1 cut(s) 1348
BstV1I GCAGC 5 cut(s) 277, 1277, 1278, 1281, 1479
BstXI CCANNNNNNTGG 1 cut(s) 1235
BsuRI GGCC 7 cut(s) 181, 858, 884, 1002, 1099, 1411, 1425
BtgI CCRYGG 1 cut(s) 245
BtsCI GGATG 8 cut(s) 316, 319, 530, 801, 934, 1016, 1081, 1100
BtsIMutI CAGTG 5 cut(s) 476, 838, 904, 1449, 1480
BtuMI TCGCGA 1 cut(s) 1348
BveI ACCTGC 1 cut(s) 50
Cac8I GCNNGC 6 cut(s) 141, 557, 1031, 1072, 1076, 1308
CaiI CAGNNNCTG 1 cut(s) 743
CciI TCATGA 2 cut(s) 1108, 1335
CfoI GCGC 1 cut(s) 644
Cfr13I GGNCC 4 cut(s) 179, 180, 1395, 1409
Csp6I GTAC 1 cut(s) 1461
CviAII CATG 9 cut(s) 76, 151, 446, 806, 1021, 1109, 1116, 1151, 1336
CviQI GTAC 1 cut(s) 1461
DdeI CTNAG 4 cut(s) 341, 618, 744, 810
DpnI GATC 6 cut(s) 35, 213, 257, 709, 1038, 1419
DpnII GATC 6 cut(s) 33, 211, 255, 707, 1036, 1417
DriI GACNNNNNGTC 1 cut(s) 454
Eam1105I GACNNNNNGTC 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 1404
Eco147I AGGCCT 1 cut(s) 858
Eco24I GRGCYC 1 cut(s) 183
Eco31I GGTCTC 1 cut(s) 196
Eco47I GGWCC 1 cut(s) 1395
Eco57I CTGAAG 2 cut(s) 888, 1158
Eco88I CYCGRG 1 cut(s) 105
Eco91I GGTNACC 1 cut(s) 800
EcoNI CCTNNNNNAGG 1 cut(s) 171
EcoO109I RGGNCCY 1 cut(s) 180
EcoO65I GGTNACC 1 cut(s) 800
EcoRII CCWGG 1 cut(s) 1234
EcoT14I CCWWGG 1 cut(s) 1404
EcoT22I ATGCAT 2 cut(s) 447, 1156
EcoT38I GRGCYC 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 1404
FaeI CATG 9 cut(s) 79, 154, 449, 809, 1024, 1112, 1119, 1154, 1339
FatI CATG 9 cut(s) 75, 150, 445, 805, 1020, 1108, 1115, 1150, 1335
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 5 cut(s) 266, 1266, 1292, 1295, 1468
FokI GGATG 8 cut(s) 306, 323, 537, 808, 921, 1003, 1068, 1087
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 5 cut(s) 266, 1266, 1292, 1295, 1468
FspBI CTAG 1 cut(s) 459
GlaI GCGC 1 cut(s) 643
GluI GCNGC 5 cut(s) 266, 1266, 1292, 1295, 1468
GsuI CTGGAG 2 cut(s) 780, 1473
HaeII RGCGCY 1 cut(s) 645
HaeIII GGCC 7 cut(s) 181, 858, 884, 1002, 1099, 1411, 1425
HapII CCGG 1 cut(s) 501
HhaI GCGC 1 cut(s) 644
Hin1II CATG 9 cut(s) 79, 154, 449, 809, 1024, 1112, 1119, 1154, 1339
Hin6I GCGC 1 cut(s) 642
HinP1I GCGC 1 cut(s) 642
HincII GTYRAC 1 cut(s) 587
HindII GTYRAC 1 cut(s) 587
HinfI GANTC 5 cut(s) 108, 1112, 1231, 1319, 1400
HpaII CCGG 1 cut(s) 501
HphI GGTGA 2 cut(s) 43, 500
Hpy166II GTNNAC 2 cut(s) 587, 1354
Hpy188I TCNGA 5 cut(s) 619, 707, 789, 868, 1012
Hpy188III TCNNGA 9 cut(s) 167, 189, 215, 812, 1109, 1182, 1316, 1336, 1347
Hpy8I GTNNAC 2 cut(s) 587, 1354
HpyAV CCTTC 5 cut(s) 22, 600, 920, 993, 1170
HpyCH4III ACNGT 5 cut(s) 249, 452, 685, 833, 899
HpyF10VI GCNNNNNNNGC 6 cut(s) 512, 855, 999, 1030, 1071, 1303
HpyF3I CTNAG 4 cut(s) 341, 618, 744, 810
Hsp92II CATG 9 cut(s) 79, 154, 449, 809, 1024, 1112, 1119, 1154, 1339
HspAI GCGC 1 cut(s) 642
Ksp22I TGATCA 1 cut(s) 33
Kzo9I GATC 6 cut(s) 33, 211, 255, 707, 1036, 1417
LmnI GCTCC 3 cut(s) 160, 503, 1260
Lsp1109I GCAGC 5 cut(s) 277, 1277, 1278, 1281, 1479
LweI GCATC 8 cut(s) 432, 515, 730, 881, 1025, 1123, 1395, 1479
MaeI CTAG 1 cut(s) 459
MaeIII GTNAC 5 cut(s) 286, 461, 488, 800, 835
MalI GATC 6 cut(s) 35, 213, 257, 709, 1038, 1419
MboI GATC 6 cut(s) 33, 211, 255, 707, 1036, 1417
MboII GAAGA 6 cut(s) 233, 364, 367, 480, 914, 1151
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 5 cut(s) 668, 912, 952, 1040, 1169
MlyI GAGTC 3 cut(s) 117, 1106, 1394
MnlI CCTC 9 cut(s) 613, 655, 783, 786, 848, 1013, 1095, 1211, 1436
Mph1103I ATGCAT 2 cut(s) 447, 1156
MroXI GAANNNNTTC 2 cut(s) 336, 930
MseI TTAA 4 cut(s) 381, 414, 545, 768
MslI CAYNNNNRTG 1 cut(s) 381
MspCI CTTAAG 1 cut(s) 544
MspI CCGG 1 cut(s) 501
MspR9I CCNGG 1 cut(s) 1236
MvaI CCWGG 1 cut(s) 1236
MvnI CGCG 1 cut(s) 1348
MwoI GCNNNNNNNGC 6 cut(s) 512, 855, 999, 1030, 1071, 1303
NdeII GATC 6 cut(s) 33, 211, 255, 707, 1036, 1417
NlaIII CATG 9 cut(s) 79, 154, 449, 809, 1024, 1112, 1119, 1154, 1339
NlaIV GGNNCC 4 cut(s) 42, 181, 816, 1410
NmuCI GTSAC 1 cut(s) 488
NruI TCGCGA 1 cut(s) 1348
NsiI ATGCAT 2 cut(s) 447, 1156
NspI RCATGY 1 cut(s) 154
PagI TCATGA 2 cut(s) 1108, 1335
PceI AGGCCT 1 cut(s) 858
PdmI GAANNNNTTC 2 cut(s) 336, 930
PfeI GAWTC 2 cut(s) 1231, 1319
PkrI GCNGC 5 cut(s) 267, 1267, 1293, 1296, 1469
PleI GAGTC 3 cut(s) 116, 1106, 1394
PpsI GAGTC 3 cut(s) 116, 1106, 1394
PshAI GACNNNNGTC 1 cut(s) 1368
Psp6I CCWGG 1 cut(s) 1234
PspEI GGTNACC 1 cut(s) 800
PspGI CCWGG 1 cut(s) 1234
PspN4I GGNNCC 4 cut(s) 42, 181, 816, 1410
PspOMI GGGCCC 1 cut(s) 179
PspPI GGNCC 4 cut(s) 179, 180, 1395, 1409
PstI CTGCAG 1 cut(s) 535
PstNI CAGNNNCTG 1 cut(s) 743
RruI TCGCGA 1 cut(s) 1348
RsaI GTAC 1 cut(s) 1462
RsaNI GTAC 1 cut(s) 1461
RseI CAYNNNNRTG 1 cut(s) 381
SaqAI TTAA 4 cut(s) 381, 414, 545, 768
SatI GCNGC 5 cut(s) 266, 1266, 1292, 1295, 1468
Sau3AI GATC 6 cut(s) 33, 211, 255, 707, 1036, 1417
Sau96I GGNCC 4 cut(s) 179, 180, 1395, 1409
SchI GAGTC 3 cut(s) 117, 1106, 1394
ScrFI CCNGG 1 cut(s) 1236
SduI GDGCHC 1 cut(s) 183
SfaNI GCATC 8 cut(s) 432, 515, 730, 881, 1025, 1123, 1395, 1479
SfcI CTRYAG 2 cut(s) 45, 531
SinI GGWCC 1 cut(s) 1395
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 4 cut(s) 215, 544, 773, 1086
SmoI CTYRAG 4 cut(s) 215, 544, 773, 1086
Sse9I AATT 5 cut(s) 668, 912, 952, 1040, 1169
SseBI AGGCCT 1 cut(s) 858
SsiI CCGC 1 cut(s) 206
SspI AATATT 1 cut(s) 751
SspMI CTAG 1 cut(s) 459
StuI AGGCCT 1 cut(s) 858
StyD4I CCNGG 1 cut(s) 1234
StyI CCWWGG 1 cut(s) 1404
TaaI ACNGT 5 cut(s) 249, 452, 685, 833, 899
TaqI TCGA 2 cut(s) 1035, 1322
TaqII GACCGA 1 cut(s) 1412
TasI AATT 5 cut(s) 668, 912, 952, 1040, 1169
TatI WGTACW 1 cut(s) 1460
TfiI GAWTC 2 cut(s) 1231, 1319
Tru1I TTAA 4 cut(s) 381, 414, 545, 768
Tru9I TTAA 4 cut(s) 381, 414, 545, 768
TscAI CASTG 5 cut(s) 483, 838, 904, 1456, 1480
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 5 cut(s) 265, 1265, 1291, 1294, 1467
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 5 cut(s) 17, 297, 681, 923, 939
TspRI CASTG 5 cut(s) 483, 838, 904, 1456, 1480
Vha464I CTTAAG 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 1395
XagI CCTNNNNNAGG 1 cut(s) 171
XapI RAATTY 2 cut(s) 952, 1169
XceI RCATGY 1 cut(s) 154
XmnI GAANNNNTTC 2 cut(s) 336, 930
XspI CTAG 1 cut(s) 459
Zsp2I ATGCAT 2 cut(s) 447, 1156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.