pycom15g29720

DNA polymerase III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015333
Physical Location & Seq
Forward (+)
26667566 .. 26668905
1340 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 708 bp
ATGGATGCTTCTACGTCATCATCCAAACAAGGCACAACAGAGATTGTGTTCTTTGACATAGAAACAAATGTACCCAATAGAGCCGGACAAAGGTTCTGGGTTTTAGAGTTTGGTGCAATTGTAGTTTGCCCTCAGAAGCTTGTTGAGCTGGAAAGTTATAGCACGCTCATTAGGCCGGGGGACTTGTCTGCTGTAGCATTGAGGTCTGGCCGGTCCGAGGGGATCACACAGGAAACTGTTGTAGATGCACCCTTGTTTGAGGAAGTTGCCGATAAGATATTCAGCATTTTGAATGGCAGGGTGTGGGCAGGCCACAACATTCGAAGATTCGATTGTGTTAGGATTAAGGAGGCCTTTGCAAAGATCGGTAGGCCTGCACCTCAGCCAGTTGGGATGATTGATTCTTTAGGTGTGTTAACTGGTAAGTTTGGAAGAAGAGCTGGAAATATGAAGATGGCAACATTGGCATCATACTTCAATCTTGGGCAGCAAAAGCACAGGAGCCTTGAAGACGTAAGGATGAACCTGGAGGTCCTCAAGAACTGTGCAACCGTGCTCTTTTTGGTACTACCACAAGAACATACATACATACATATATATATATATATATATATATATGTATGTATGGTAAAGAATCTGTTGTGTAGAGCCCAAGGAAATCAACCTCTTAACAACATCCTCAAGCATTCTCATTCCCTACTACGGTGA

Protein Analysis

236

Amino Acids

26.4

Weight (kDa)

9.13

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 16 - 179 3.3e-27 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015333)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39810
fragaria_vesca FvH4_1g21490
malus_domestica MD15G1329100.v1.1
prunus_persica Prupe.6G179800_v2.0.a1
pyrus_communis pycom01g05230 pycom15g29710 pycom15g29720
rosa_chinensis RchiOBHm_Chr2g0115371
rosa_laevigata RLG00000018180
rosa_roxburghii Rroxscaffold_2G00130860
rosa_rugosa Rorug02G0195900
rosa_samantha Rh2AG254300 Rh2BG264500 Rh2CG259000 Rh2DG262400
rosa_wichuraiana Rw2G019860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 230
AcoI YGGCCR 1 cut(s) 208
AfaI GTAC 2 cut(s) 72, 567
AfiI CCNNNNNNNGG 3 cut(s) 90, 217, 702
AgsI TTSAA 3 cut(s) 292, 478, 509
AjnI CCWGG 1 cut(s) 525
AluBI AGCT 3 cut(s) 139, 148, 440
AluI AGCT 3 cut(s) 139, 148, 440
Alw21I GWGCWC 1 cut(s) 558
AlwI GGATC 1 cut(s) 230
AoxI GGCC 5 cut(s) 173, 208, 310, 351, 371
ApeKI GCWGC 1 cut(s) 487
AspS9I GGNCC 2 cut(s) 213, 532
AsuC2I CCSGG 1 cut(s) 177
AsuII TTCGAA 1 cut(s) 322
AvaII GGWCC 2 cut(s) 213, 532
BaeI ACNNNNGTAYC 2 cut(s) 54, 87
BanII GRGCYC 1 cut(s) 652
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 1 cut(s) 558
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 1 cut(s) 499
BccI CCATC 1 cut(s) 448
BciT130I CCWGG 1 cut(s) 527
BcnI CCSGG 1 cut(s) 177
BfmI CTRYAG 1 cut(s) 192
BisI GCNGC 1 cut(s) 488
BlsI GCNGC 1 cut(s) 489
Bme1390I CCNGG 2 cut(s) 177, 527
Bme18I GGWCC 2 cut(s) 213, 532
BmgT120I GGNCC 2 cut(s) 213, 532
BmiI GGNNCC 1 cut(s) 503
BmrFI CCNGG 2 cut(s) 177, 527
BmsI GCATC 2 cut(s) 235, 476
BpiI GAAGAC 1 cut(s) 516
BpmI CTGGAG 1 cut(s) 548
Bpu10I CCTNAGC 1 cut(s) 381
Bpu14I TTCGAA 1 cut(s) 322
BpuEI CTTGAG 2 cut(s) 521, 665
BpuMI CCSGG 1 cut(s) 177
BsaJI CCNNGG 3 cut(s) 176, 216, 652
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 217, 702
Bse118I RCCGGY 1 cut(s) 210
Bse1I ACTGG 2 cut(s) 386, 424
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 3 cut(s) 176, 216, 652
BseGI GGATG 5 cut(s) 10, 20, 399, 525, 675
BseLI CCNNNNNNNGG 3 cut(s) 90, 217, 702
BseMII CTCAG 2 cut(s) 146, 395
BseNI ACTGG 2 cut(s) 386, 424
BseXI GCAGC 1 cut(s) 499
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 5 cut(s) 175, 210, 312, 353, 373
BsiHKAI GWGCWC 1 cut(s) 558
BsiSI CCGG 3 cut(s) 84, 176, 211
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 3 cut(s) 90, 217, 702
BsmFI GGGAC 1 cut(s) 194
BsmI GAATGC 1 cut(s) 685
BsnI GGCC 5 cut(s) 175, 210, 312, 353, 373
Bsp119I TTCGAA 1 cut(s) 322
Bsp1286I GDGCHC 2 cut(s) 558, 652
Bsp143I GATC 2 cut(s) 222, 363
BspANI GGCC 5 cut(s) 175, 210, 312, 353, 373
BspCNI CTCAG 2 cut(s) 145, 394
BspLI GGNNCC 1 cut(s) 503
BspPI GGATC 1 cut(s) 230
BspQI GCTCTTC 1 cut(s) 430
BspT104I TTCGAA 1 cut(s) 322
BsrFI RCCGGY 1 cut(s) 210
BsrI ACTGG 2 cut(s) 386, 424
BssAI RCCGGY 1 cut(s) 210
BssECI CCNNGG 3 cut(s) 176, 216, 652
BssMI GATC 2 cut(s) 222, 363
BssT1I CCWWGG 1 cut(s) 652
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 4 cut(s) 238, 545, 553, 705
Bst6I CTCTTC 1 cut(s) 430
BstBI TTCGAA 1 cut(s) 322
BstC8I GCNNGC 3 cut(s) 164, 310, 375
BstDEI CTNAG 2 cut(s) 132, 381
BstF5I GGATG 5 cut(s) 10, 20, 399, 525, 675
BstKTI GATC 2 cut(s) 225, 366
BstMBI GATC 2 cut(s) 222, 363
BstMWI GCNNNNNNNGC 4 cut(s) 145, 172, 464, 493
BstNI CCWGG 1 cut(s) 527
BstSCI CCNGG 2 cut(s) 175, 525
BstSFI CTRYAG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 499
BstV2I GAAGAC 1 cut(s) 516
BsuRI GGCC 5 cut(s) 175, 210, 312, 353, 373
BtsCI GGATG 5 cut(s) 10, 20, 399, 525, 675
Cac8I GCNNGC 3 cut(s) 164, 310, 375
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 2 cut(s) 213, 532
CpoI CGGWCCG 1 cut(s) 213
Csp6I GTAC 2 cut(s) 71, 566
CspI CGGWCCG 1 cut(s) 213
CviQI GTAC 2 cut(s) 71, 566
DdeI CTNAG 2 cut(s) 132, 381
DpnI GATC 2 cut(s) 224, 365
DpnII GATC 2 cut(s) 222, 363
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 430
EarI CTCTTC 1 cut(s) 430
Eco130I CCWWGG 1 cut(s) 652
Eco147I AGGCCT 2 cut(s) 353, 373
Eco24I GRGCYC 1 cut(s) 652
Eco47I GGWCC 2 cut(s) 213, 532
EcoO109I RGGNCCY 1 cut(s) 532
EcoRII CCWGG 1 cut(s) 525
EcoT14I CCWWGG 1 cut(s) 652
EcoT38I GRGCYC 1 cut(s) 652
ErhI CCWWGG 1 cut(s) 652
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 1 cut(s) 194
Fnu4HI GCNGC 1 cut(s) 488
FokI GGATG 5 cut(s) 7, 17, 406, 532, 662
FriOI GRGCYC 1 cut(s) 652
Fsp4HI GCNGC 1 cut(s) 488
GluI GCNGC 1 cut(s) 488
GsuI CTGGAG 1 cut(s) 548
HaeIII GGCC 5 cut(s) 175, 210, 312, 353, 373
HapII CCGG 3 cut(s) 84, 176, 211
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 3 cut(s) 327, 401, 634
HpaI GTTAAC 1 cut(s) 417
HpaII CCGG 3 cut(s) 84, 176, 211
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 2 cut(s) 135, 217
Hpy188III TCNNGA 1 cut(s) 538
Hpy8I GTNNAC 1 cut(s) 417
HpyCH4III ACNGT 4 cut(s) 238, 545, 553, 705
HpyCH4IV ACGT 2 cut(s) 14, 513
HpyCH4V TGCA 5 cut(s) 116, 248, 359, 377, 548
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 172, 464, 493
HpyF3I CTNAG 2 cut(s) 132, 381
HpySE526I ACGT 2 cut(s) 14, 513
KspAI GTTAAC 1 cut(s) 417
Kzo9I GATC 2 cut(s) 222, 363
LguI GCTCTTC 1 cut(s) 430
LmnI GCTCC 1 cut(s) 501
Lsp1109I GCAGC 1 cut(s) 499
LweI GCATC 2 cut(s) 235, 476
MaeII ACGT 2 cut(s) 14, 513
MalI GATC 2 cut(s) 224, 365
MboI GATC 2 cut(s) 222, 363
MboII GAAGA 5 cut(s) 336, 444, 447, 463, 521
MfeI CAATTG 1 cut(s) 117
MhlI GDGCHC 2 cut(s) 558, 652
MluCI AATT 1 cut(s) 117
MseI TTAA 3 cut(s) 345, 416, 669
MspI CCGG 3 cut(s) 84, 176, 211
MspR9I CCNGG 2 cut(s) 177, 527
MunI CAATTG 1 cut(s) 117
Mva1269I GAATGC 1 cut(s) 685
MvaI CCWGG 1 cut(s) 527
MwoI GCNNNNNNNGC 4 cut(s) 145, 172, 464, 493
NciI CCSGG 1 cut(s) 177
NdeII GATC 2 cut(s) 222, 363
NlaIV GGNNCC 1 cut(s) 503
NspV TTCGAA 1 cut(s) 322
PceI AGGCCT 2 cut(s) 353, 373
PciSI GCTCTTC 1 cut(s) 430
PctI GAATGC 1 cut(s) 685
PfeI GAWTC 3 cut(s) 327, 401, 634
PkrI GCNGC 1 cut(s) 489
PpuMI RGGWCCY 1 cut(s) 532
Psp5II RGGWCCY 1 cut(s) 532
Psp6I CCWGG 1 cut(s) 525
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 1 cut(s) 503
PspPI GGNCC 2 cut(s) 213, 532
PspPPI RGGWCCY 1 cut(s) 532
RsaI GTAC 2 cut(s) 72, 567
RsaNI GTAC 2 cut(s) 71, 566
Rsr2I CGGWCCG 1 cut(s) 213
RsrII CGGWCCG 1 cut(s) 213
SapI GCTCTTC 1 cut(s) 430
SaqAI TTAA 3 cut(s) 345, 416, 669
SatI GCNGC 1 cut(s) 488
Sau3AI GATC 2 cut(s) 222, 363
Sau96I GGNCC 2 cut(s) 213, 532
ScrFI CCNGG 2 cut(s) 177, 527
SduI GDGCHC 2 cut(s) 558, 652
SfaNI GCATC 2 cut(s) 235, 476
SfcI CTRYAG 1 cut(s) 192
SfuI TTCGAA 1 cut(s) 322
SinI GGWCC 2 cut(s) 213, 532
SmlI CTYRAG 2 cut(s) 536, 680
SmoI CTYRAG 2 cut(s) 536, 680
Sse9I AATT 1 cut(s) 117
SseBI AGGCCT 2 cut(s) 353, 373
StuI AGGCCT 2 cut(s) 353, 373
StyD4I CCNGG 2 cut(s) 175, 525
StyI CCWWGG 1 cut(s) 652
TaaI ACNGT 4 cut(s) 238, 545, 553, 705
TaiI ACGT 2 cut(s) 17, 516
TaqI TCGA 2 cut(s) 322, 330
TasI AATT 1 cut(s) 117
TfiI GAWTC 3 cut(s) 327, 401, 634
Tru1I TTAA 3 cut(s) 345, 416, 669
Tru9I TTAA 3 cut(s) 345, 416, 669
TseI GCWGC 1 cut(s) 487
TspDTI ATGAA 2 cut(s) 464, 536
VpaK11BI GGWCC 2 cut(s) 213, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.