pycom15g30970

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009147
Physical Location & Seq
Forward (+)
29029605 .. 29034462
4858 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3666 bp
ATGAACTCATCTAGTCTAGAAATCAGAGTTGGAGGAGCTGCATTACTTATTTGTGCAGCAAAAGAGCACAAGCAGAAGGCACTCGAAGTACTTGATGTGTCAGGATATTTGGAACCATTAACGTATGCTTTAGTGGACATGGTGAAGCGAAAGTCTAGCTGCTCCTTTCCAGAAATTGAAGTCAGAACTCCTAGAGGTTTTATTGAAAGAACTGCATTCCACGAAGTTAATGTCCCTGATCCAGCCATTGTTTTGGGGGGTACTGTTGCCTTGTGGTTGCTATGCATAATTGGTTCCTTTCATGCCAAAAGCAAACTCACCATTATGGAAGCTGGTGGTCTTGAGGTTCTATCTGAAAAGCTTGCAGGTTACACTTCCAATCCACAGGCAGAATATGAGGATACAGAAGGTATATGGATTAGTGCCCTGGTCCTGGCTATTCTGTTCGAAGATGCAAATGTTGTTCTGTCACCTGTAACAATGCGCATCATTCCTTCACTTGCTCTTCTTTTGAAATCGGATGAGATGATTGATAGGTTCTTTGCTGCCCAGTCAATGGCCACTCTTGTTTATAATGGTAGTAAAGGAATAATTCTTGCCCTTGCAAATTCAGGTGCTGTTGCTGGATTGATAACTCTTATTGGTTATGTAGAGTCCGACGTGCCTAACCTTGTTACTTTATCAGAAGAGTTTTCTCTGGTGCGAAATCCTGATCAAGTTGTTTTGGAGTGCCTTTTTGATTTTGAAGACATAAGAGTTGGATCAACAGCACGTAAATCTATACCTCTCTTGGTGGATCTCTTGAGACCAATGCCAGAACGGCCCGGTGCCCCTCCAATTGCTGTTAAACTCTTGACTCGTATCGCCAATGGAAGTGATACAAATAAGTTAATTGTGGGTGAAGCTGGAGCTCTGGATGCTCTGACAAAGTACCTGTCTCTAAGTCCACAAGACTCCACCGAAGCCACAATAACTGAATTATTCAGAATATTATTTAGCAATCCTGATCTCATTCGATATGAAGCATCAGCTAGTTCATTGAATCAACTCATAGCTGTTTTGCGACTGGGTTCAAGAAATGCAAGATATAGTGCTGCCAGAGCTCTTCATGAACTATTTGGTGCTGAGAACATTAGAGATTCTGATTTAGCCAGGCACTCAATTCAGCCATTGGTTGACATGCTTAACGCTGCATCAGAGAGTGAACAAGAGGCTGCCCTCATTGCGTTAATAAAGTTGACTTCAGGAAATTCTTCTAAAGCAGCTTTGTTGACTGATGTGGAGGGAAATCCAATGGAGAGTTTGTACAAAATACTGTCTTCTGCTTCATCCTTGGAATTAAAGAGAACTGCTGCACAACTCTGTTATACTTTGTTTGATAATACTGAAGTCAGAGGAAATCCAATTGCCTCAGAATGCATAGAGCCCCTTATATCACTGATGCATTCTGATGCTACTACAGCTGTAGAAGCTGGAGTTTGTGCTTTTGAAAAATTGTTGGATGATGAACACCGGGTGGAGCTTGCAATGGTCTATAATGTTGTGGATCTCCTTGTGGGATTAGTTTCTGGAACAAGCATGCAGCTCATTGAGGCCAGTGTCTGTTCTCTCGTAAAGTTGGGGAAAGACCGGACCCCATGCAAATTGGATATGGTCAATGCTGGAATCATTGATAAATGTCTTGAACTACTACCTGTTGCACCCAGTTCATTATGCTCTTCCATAGCTGAATTGTTCCGCATTTTAACAAACAGTAATGCAATTGCTAGAAGTTTGGCTGCTGCACAAATTGTAGAACCTCTTTTTATAGTTTTACAACATCCAGATTTCAGTTTATGGGGACAGCACAGTGCATTACAAGCACTAGTAAATATTTTGGAGAAGCCACAGAGCCTTGCAACGTTAAAGCTTACACCTAGCCAAGTGATCGAGCCTCTTATTTCATTTCTGGAATCCCCATCTCAAGCCATTCAGCAGCTTGGCACAGAATTGCTATCTCATCTTCTTGCACAAGAACATTTTCAGCTAGATATTACAACAAAAAATGCAGTTGTTCCACTTGTTCAGCTTGCAGGAATTGGAATATTAAACTTACAGCAGACAGCGGTAAAGGCATTGGAAATAATCTCCACAAGCTGGCCAAAGGCAGTTGCTGATGCTGGTGGTATATTTGAGCTTGGAAAGGTTATTATTCAAGATGACCCTCAGCCACCTCATGCCCTGTGGGAATCAGCTGCTTTAGTTCTCTCAAATGTTCTGCACTTCAACGCTGAATACTATTTTAAGGTTCCTGTGGTGGTTCTTGTTAAAATGTTGCATTCAACAGTGGACAGCACCATCACGGTGGCCCTCAATGCGTTGCTTGTTCATGAAAGGAGTGATAATTTAAGTGCTGAACAGATGACTGATGGTGGTGCTGTAGAAGCCTTGTTGGACCTTTTACGGTCTCATCAATGCGAAGAAGCATCTGGAAGATTACTTGAAGCTCTATTTAACAATGTCAGGATACGACAAATGAAGGTCTCTAAGTATGCAATAGCACCGCTATCACGGTATCTGTTGGATCCACAAACCAAATCAGAGTCTGGAAAACTTCTTGCTGCTCTTGCTCTTGGAGACCTTTCCCAACATGAAGGGCTTGCGAGAGCCAGAGAGTCCGTTTCTGCGTGTCGTGCATTGGTAAGCTTGCTTGAAGAACAGCCAACAGAAGAGATGAAAATGGTGTCAATTTGTGCATTGCAAAACTTCGTCATGAACAGCCGAACTAATAGGCGAGCTGTCGCAGAAGCAGGGGGCATACTGATTATTCAAGAACTACTACTGTCTCCCAATGCAGAAACTGCTGGGCAGGCAGCATTGCTTATCAAATTTTTGTTTTCTAATCACACACTCCAAGAGTATGTATCAAATGAACTTATCAGATCTTTGACAGCTGCATTGGAGAGAGAGTTGTGGTCCTCAGCAACTATTAATGAAGAGGTCTTGAGAGCCTTACATATGATATTCGTCAACTTCCCAAAGCTCCATATTTCTGAAGCAGCAACTCTCTGCATTCCCAATTTGATCGGAGTTCTTAAATCTGGTAGTGACACGGCTCAGGATGTTGTTTTGGACACCCTTTCCCTGTTGAGACATTCTTGGTCAACTATGCCAATAGATATTGCAAAGTCTCAGGCTGTGATTGCTGCTGAAGCCATTCCCATCCTACAAATGCTCATGAAAACCTGTCCTCCAAGTTTCCATGAGAGAGCAGACAGCCTGTTGCATTGCTTACCAGGTTGTTTGACGGTTACAATTAAGCGGGGGAACAACCTTAAACAGGCCATGGGAGGCACAAATGCTTTTTGTCGATTGACAATAGGCAATGGCCCTCCTCGACAAACCAAGGTAGTGAGCCACAGTACCTCTCCTGAGTGGAAAGAAGGCTTTACGTGGGCATTTGATGTGCCTCCAAAGGGACAGAAACTCCATATCATTTGCAAAAGCAAAAATACCTTCGGAAAGACAACTCTGGGAAGAGTGACGATCCAAATTGACAAAGTCGTTAGTGAAGGAGTATACAGTGGATTGTTCAGTCTAAATCATGACAGCAACAAGGATGGGTCTTCCCGTACACTAGAGATCGAGATTATCTGGTCAAACAGAATGGCTGACGAAGAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000271 GO:0000902 GO:0002682 GO:0002683 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009664 GO:0009826 GO:0009832 GO:0009987 GO:0010330 GO:0010383 GO:0010941 GO:0015630 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030243 GO:0030244 GO:0030863 GO:0030981 GO:0032446 GO:0032502 GO:0032989 GO:0032991 GO:0033036 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042546 GO:0042742 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045229 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048589 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051239 GO:0051273 GO:0051274 GO:0051641 GO:0051704 GO:0051707 GO:0052324 GO:0052541 GO:0055028 GO:0060548 GO:0060560 GO:0065007 GO:0070589 GO:0070592 GO:0070647 GO:0070696 GO:0070727 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0098542 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1902494 GO:1990234 GO:1990778 GO:2000026 GO:2000028 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

1222

Amino Acids

132.56

Weight (kDa)

5.35

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_ARMC5 PF24768 347 - 487 3.1e-06 ARMC5
C2 PF00168 1094 - 1192 3.5e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
Acc16I TGCGCA 1 cut(s) 485
Acc36I ACCTGC 1 cut(s) 356
AccB1I GGYRCC 1 cut(s) 827
AccB7I CCANNNNNTGG 2 cut(s) 556, 2136
AccI GTMKAC 1 cut(s) 3558
AciI CCGC 4 cut(s) 1738, 2105, 2543, 3301
AclI AACGTT 1 cut(s) 1901
AclWI GGATC 7 cut(s) 233, 769, 804, 1554, 2558, 2571, 3520
AcoI YGGCCR 2 cut(s) 558, 2138
AcsI RAATTY 3 cut(s) 607, 1249, 2867
AcuI CTGAAG 4 cut(s) 1227, 1407, 3054, 3210
AdeI CACNNNGTG 1 cut(s) 1516
AfaI GTAC 6 cut(s) 90, 262, 932, 1307, 3403, 3613
AfiI CCNNNNNNNGG 5 cut(s) 433, 556, 2136, 3319, 3455
AhlI ACTAGT 1 cut(s) 1864
AjiI CACGTC 1 cut(s) 661
AjnI CCWGG 4 cut(s) 426, 432, 1151, 3274
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
Alw21I GWGCWC 3 cut(s) 69, 913, 1105
Alw26I GTCTC 8 cut(s) 799, 942, 2451, 2527, 2610, 2829, 3124, 3174
AlwI GGATC 7 cut(s) 233, 769, 804, 1554, 2558, 2571, 3520
AlwNI CAGNNNCTG 5 cut(s) 617, 1602, 2153, 2585, 2840
AoxI GGCC 7 cut(s) 558, 821, 1593, 2138, 2346, 3321, 3367
ApoI RAATTY 3 cut(s) 607, 1249, 2867
ArsI GACNNNNNNTTYG 4 cut(s) 216, 248, 920, 952
AseI ATTAAT 1 cut(s) 2970
Asp700I GAANNNNTTC 3 cut(s) 1252, 2019, 3195
AspLEI GCGC 1 cut(s) 486
AspS9I GGNCC 7 cut(s) 430, 822, 1632, 2347, 2434, 2955, 3368
AsuC2I CCSGG 2 cut(s) 825, 1514
AsuHPI GGTGA 4 cut(s) 154, 310, 462, 911
AsuII TTCGAA 1 cut(s) 447
AvaII GGWCC 4 cut(s) 430, 1632, 2434, 2955
BaeGI GKGCMC 2 cut(s) 427, 832
BalI TGGCCA 2 cut(s) 560, 2140
BamHI GGATCC 1 cut(s) 2563
BanI GGYRCC 1 cut(s) 827
BanII GRGCYC 3 cut(s) 913, 1105, 1428
BbsI GAAGAC 3 cut(s) 753, 1311, 3597
Bbv12I GWGCWC 3 cut(s) 69, 913, 1105
BbvCI CCTCAGC 2 cut(s) 2205, 2959
BccI CCATC 5 cut(s) 1966, 2345, 2402, 3209, 3593
BceAI ACGGC 2 cut(s) 836, 3108
BciT130I CCWGG 4 cut(s) 428, 434, 1153, 3276
BciVI GTATCC 2 cut(s) 394, 2499
BclI TGATCA 1 cut(s) 712
BcnI CCSGG 2 cut(s) 825, 1514
BcoDI GTCTC 8 cut(s) 799, 942, 2451, 2527, 2610, 2829, 3124, 3174
BcuI ACTAGT 1 cut(s) 1864
BfmI CTRYAG 3 cut(s) 1458, 1464, 2418
BfuAI ACCTGC 1 cut(s) 356
BfuI GTATCC 2 cut(s) 394, 2499
BglI GCCNNNNNGGC 1 cut(s) 820
BglII AGATCT 1 cut(s) 2921
BmcAI AGTACT 1 cut(s) 90
Bme1390I CCNGG 6 cut(s) 428, 434, 825, 1153, 1514, 3276
Bme18I GGWCC 4 cut(s) 430, 1632, 2434, 2955
BmgBI CACGTC 1 cut(s) 661
BmgT120I GGNCC 7 cut(s) 430, 822, 1632, 2347, 2434, 2955, 3368
BmiI GGNNCC 6 cut(s) 114, 295, 829, 1634, 2289, 2565
BmrFI CCNGG 6 cut(s) 428, 434, 825, 1153, 1514, 3276
BmrI ACTGGG 3 cut(s) 544, 1076, 1698
BmsI GCATC 9 cut(s) 442, 495, 907, 1034, 1202, 1431, 1441, 2146, 2474
BmuI ACTGGG 3 cut(s) 544, 1076, 1698
BpiI GAAGAC 3 cut(s) 753, 1311, 3597
BpmI CTGGAG 2 cut(s) 927, 1494
Bpu10I CCTNAGC 3 cut(s) 2205, 2959, 3096
Bpu14I TTCGAA 1 cut(s) 447
BpuEI CTTGAG 4 cut(s) 362, 823, 1947, 3004
BpuMI CCSGG 2 cut(s) 825, 1514
BsaAI YACGTR 2 cut(s) 773, 3432
BsaI GGTCTC 4 cut(s) 799, 2451, 2527, 2610
BsaJI CCNNGG 4 cut(s) 426, 1332, 3324, 3384
BsaWI WCCGGW 1 cut(s) 1629
BsaXI ACNNNNNCTCC 8 cut(s) 1289, 1319, 2933, 2963, 3214, 3244, 3450, 3480
Bsc4I CCNNNNNNNGG 5 cut(s) 433, 556, 2136, 3319, 3455
Bse1I ACTGG 4 cut(s) 550, 1071, 1596, 1704
Bse3DI GCAATG 6 cut(s) 1221, 1533, 2735, 2855, 3265, 3370
BseBI CCWGG 4 cut(s) 428, 434, 1153, 3276
BseDI CCNNGG 4 cut(s) 426, 1332, 3324, 3384
BseGI GGATG 8 cut(s) 526, 922, 1328, 1507, 1819, 3106, 3201, 3604
BseLI CCNNNNNNNGG 5 cut(s) 433, 556, 2136, 3319, 3455
BseMI GCAATG 6 cut(s) 1221, 1533, 2735, 2855, 3265, 3370
BseMII CTCAG 7 cut(s) 1116, 1425, 2219, 2973, 3110, 3185, 3402
BseNI ACTGG 4 cut(s) 550, 1071, 1596, 1704
BseRI GAGGAG 2 cut(s) 48, 3363
BseSI GKGCMC 2 cut(s) 427, 832
BseYI CCCAGC 1 cut(s) 2843
BsgI GTGCAG 4 cut(s) 75, 1338, 1767, 2243
BshFI GGCC 7 cut(s) 560, 823, 1595, 2140, 2348, 3323, 3369
BshNI GGYRCC 1 cut(s) 827
BsiHKAI GWGCWC 3 cut(s) 69, 913, 1105
BsiSI CCGG 3 cut(s) 825, 1513, 1630
BslFI GGGAC 3 cut(s) 218, 1854, 3471
BslI CCNNNNNNNGG 5 cut(s) 433, 556, 2136, 3319, 3455
BsmAI GTCTC 8 cut(s) 799, 942, 2451, 2527, 2610, 2829, 3124, 3174
BsmFI GGGAC 3 cut(s) 218, 1854, 3471
BsmI GAATGC 5 cut(s) 215, 1421, 1444, 2317, 3051
BsnI GGCC 7 cut(s) 560, 823, 1595, 2140, 2348, 3323, 3369
Bso31I GGTCTC 4 cut(s) 799, 2451, 2527, 2610
Bsp119I TTCGAA 1 cut(s) 447
Bsp1286I GDGCHC 6 cut(s) 69, 427, 832, 913, 1105, 1428
Bsp1407I TGTACA 1 cut(s) 1305
Bsp19I CCATGG 1 cut(s) 3324
BspACI CCGC 4 cut(s) 1738, 2105, 2543, 3301
BspANI GGCC 7 cut(s) 560, 823, 1595, 2140, 2348, 3323, 3369
BspCNI CTCAG 7 cut(s) 1117, 1424, 2218, 2972, 3109, 3184, 3403
BspHI TCATGA 5 cut(s) 1108, 2368, 2751, 3216, 3583
BspLI GGNNCC 6 cut(s) 114, 295, 829, 1634, 2289, 2565
BspMI ACCTGC 1 cut(s) 356
BspPI GGATC 7 cut(s) 233, 769, 804, 1554, 2558, 2571, 3520
BspQI GCTCTTC 3 cut(s) 510, 1110, 1723
BspT104I TTCGAA 1 cut(s) 447
BspT107I GGYRCC 1 cut(s) 827
BspTNI GGTCTC 4 cut(s) 799, 2451, 2527, 2610
BsrDI GCAATG 6 cut(s) 1221, 1533, 2735, 2855, 3265, 3370
BsrGI TGTACA 1 cut(s) 1305
BsrI ACTGG 4 cut(s) 550, 1071, 1596, 1704
BssECI CCNNGG 4 cut(s) 426, 1332, 3324, 3384
BssNAI GTATAC 1 cut(s) 3559
BssT1I CCWWGG 3 cut(s) 1332, 3324, 3384
Bst1107I GTATAC 1 cut(s) 3559
Bst2UI CCWGG 4 cut(s) 428, 434, 1153, 3276
Bst6I CTCTTC 7 cut(s) 510, 681, 1110, 1723, 2703, 2970, 3511
BstAPI GCANNNNNTGC 1 cut(s) 2840
BstAUI TGTACA 1 cut(s) 1305
BstBAI YACGTR 2 cut(s) 773, 3432
BstBI TTCGAA 1 cut(s) 447
BstC8I GCNNGC 9 cut(s) 363, 1524, 1580, 2070, 2138, 2640, 2687, 2775, 2850
BstDEI CTNAG 9 cut(s) 941, 1125, 1411, 2205, 2526, 2959, 3096, 3171, 3411
BstDSI CCRYGG 1 cut(s) 3324
BstENI CCTNNNNNAGG 1 cut(s) 3317
BstF5I GGATG 8 cut(s) 526, 922, 1328, 1507, 1819, 3106, 3201, 3604
BstHHI GCGC 1 cut(s) 486
BstMAI GTCTC 8 cut(s) 799, 942, 2451, 2527, 2610, 2829, 3124, 3174
BstNI CCWGG 4 cut(s) 428, 434, 1153, 3276
BstNSI RCATGY 2 cut(s) 1183, 1582
BstSCI CCNGG 6 cut(s) 426, 432, 823, 1151, 1512, 3274
BstSFI CTRYAG 3 cut(s) 1458, 1464, 2418
BstSLI GKGCMC 2 cut(s) 427, 832
BstV2I GAAGAC 3 cut(s) 753, 1311, 3597
BstX2I RGATCY 4 cut(s) 796, 1546, 2563, 2921
BstXI CCANNNNNNTGG 2 cut(s) 253, 2344
BstYI RGATCY 4 cut(s) 796, 1546, 2563, 2921
BstZ17I GTATAC 1 cut(s) 3559
BsuI GTATCC 2 cut(s) 394, 2499
BsuRI GGCC 7 cut(s) 560, 823, 1595, 2140, 2348, 3323, 3369
BtgI CCRYGG 1 cut(s) 3324
BtrI CACGTC 1 cut(s) 661
BtsCI GGATG 8 cut(s) 526, 922, 1328, 1507, 1819, 3106, 3201, 3604
BtsIMutI CAGTG 5 cut(s) 1436, 1603, 1855, 2331, 3568
BveI ACCTGC 1 cut(s) 356
Cac8I GCNNGC 9 cut(s) 363, 1524, 1580, 2070, 2138, 2640, 2687, 2775, 2850
CaiI CAGNNNCTG 5 cut(s) 617, 1602, 2153, 2585, 2840
CciI TCATGA 5 cut(s) 1108, 2368, 2751, 3216, 3583
CfoI GCGC 1 cut(s) 486
Cfr13I GGNCC 7 cut(s) 430, 822, 1632, 2347, 2434, 2955, 3368
CsiI ACCWGGT 1 cut(s) 3274
Csp6I GTAC 6 cut(s) 89, 261, 931, 1306, 3402, 3612
CviQI GTAC 6 cut(s) 89, 261, 931, 1306, 3402, 3612
DdeI CTNAG 9 cut(s) 941, 1125, 1411, 2205, 2526, 2959, 3096, 3171, 3411
DraIII CACNNNGTG 1 cut(s) 1516
EaeI YGGCCR 2 cut(s) 558, 2138
Eam1104I CTCTTC 7 cut(s) 510, 681, 1110, 1723, 2703, 2970, 3511
EarI CTCTTC 7 cut(s) 510, 681, 1110, 1723, 2703, 2970, 3511
Ecl136II GAGCTC 2 cut(s) 911, 1103
Eco130I CCWWGG 3 cut(s) 1332, 3324, 3384
Eco24I GRGCYC 3 cut(s) 913, 1105, 1428
Eco31I GGTCTC 4 cut(s) 799, 2451, 2527, 2610
Eco47I GGWCC 4 cut(s) 430, 1632, 2434, 2955
Eco53kI GAGCTC 2 cut(s) 911, 1103
Eco57I CTGAAG 4 cut(s) 1227, 1407, 3054, 3210
EcoICRI GAGCTC 2 cut(s) 911, 1103
EcoNI CCTNNNNNAGG 1 cut(s) 3317
EcoRII CCWGG 4 cut(s) 426, 432, 1151, 3274
EcoT14I CCWWGG 3 cut(s) 1332, 3324, 3384
EcoT22I ATGCAT 3 cut(s) 287, 1421, 1446
EcoT38I GRGCYC 3 cut(s) 913, 1105, 1428
ErhI CCWWGG 3 cut(s) 1332, 3324, 3384
FaqI GGGAC 3 cut(s) 218, 1854, 3471
FauI CCCGC 1 cut(s) 3294
FauNDI CATATG 1 cut(s) 2997
FbaI TGATCA 1 cut(s) 712
FblI GTMKAC 1 cut(s) 3558
FokI GGATG 8 cut(s) 533, 929, 1315, 1514, 1806, 3113, 3188, 3611
FriOI GRGCYC 3 cut(s) 913, 1105, 1428
FspAI RTGCGCAY 1 cut(s) 485
FspI TGCGCA 1 cut(s) 485
GlaI GCGC 1 cut(s) 485
GsaI CCCAGC 1 cut(s) 2847
GsuI CTGGAG 2 cut(s) 927, 1494
HaeIII GGCC 7 cut(s) 560, 823, 1595, 2140, 2348, 3323, 3369
HapII CCGG 3 cut(s) 825, 1513, 1630
HhaI GCGC 1 cut(s) 486
Hin6I GCGC 1 cut(s) 484
HinP1I GCGC 1 cut(s) 484
HincII GTYRAC 5 cut(s) 1177, 1239, 1272, 3010, 3144
HindII GTYRAC 5 cut(s) 1177, 1239, 1272, 3010, 3144
HindIII AAGCTT 3 cut(s) 359, 1907, 2683
HpaII CCGG 3 cut(s) 825, 1513, 1630
HphI GGTGA 4 cut(s) 154, 310, 462, 911
Hpy99I CGWCG 1 cut(s) 662
HpyAV CCTTC 8 cut(s) 70, 401, 504, 2512, 2627, 3416, 3505, 3545
HpyCH4IV ACGT 5 cut(s) 122, 660, 772, 1901, 3431
HpyF3I CTNAG 9 cut(s) 941, 1125, 1411, 2205, 2526, 2959, 3096, 3171, 3411
HpySE526I ACGT 5 cut(s) 122, 660, 772, 1901, 3431
HspAI GCGC 1 cut(s) 484
Ksp22I TGATCA 1 cut(s) 712
LguI GCTCTTC 3 cut(s) 510, 1110, 1723
LmnI GCTCC 5 cut(s) 35, 167, 908, 1519, 3027
LweI GCATC 9 cut(s) 442, 495, 907, 1034, 1202, 1431, 1441, 2146, 2474
MabI ACCWGGT 1 cut(s) 3274
MaeII ACGT 5 cut(s) 122, 660, 772, 1901, 3431
MaeIII GTNAC 7 cut(s) 368, 468, 475, 673, 3086, 3289, 3520
MfeI CAATTG 3 cut(s) 837, 1404, 1761
MflI RGATCY 4 cut(s) 796, 1546, 2563, 2921
MhlI GDGCHC 6 cut(s) 69, 427, 832, 913, 1105, 1428
MlsI TGGCCA 2 cut(s) 560, 2140
MluNI TGGCCA 2 cut(s) 560, 2140
MlyI GAGTC 5 cut(s) 662, 850, 947, 2591, 2663
MmeI TCCRAC 6 cut(s) 10, 681, 739, 1479, 2412, 2541
Mox20I TGGCCA 2 cut(s) 560, 2140
Mph1103I ATGCAT 3 cut(s) 287, 1421, 1446
MroXI GAANNNNTTC 3 cut(s) 1252, 2019, 3195
MscI TGGCCA 2 cut(s) 560, 2140
MslI CAYNNNNRTG 3 cut(s) 323, 1449, 2342
Msp20I TGGCCA 2 cut(s) 560, 2140
MspA1I CMGCKG 4 cut(s) 1463, 2105, 2234, 2933
MspI CCGG 3 cut(s) 825, 1513, 1630
MspR9I CCNGG 6 cut(s) 428, 434, 825, 1153, 1514, 3276
MunI CAATTG 3 cut(s) 837, 1404, 1761
Mva1269I GAATGC 5 cut(s) 215, 1421, 1444, 2317, 3051
MvaI CCWGG 4 cut(s) 428, 434, 1153, 3276
NciI CCSGG 2 cut(s) 825, 1514
NcoI CCATGG 1 cut(s) 3324
NdeI CATATG 1 cut(s) 2997
NlaIV GGNNCC 6 cut(s) 114, 295, 829, 1634, 2289, 2565
NmuCI GTSAC 3 cut(s) 468, 3086, 3520
NsbI TGCGCA 1 cut(s) 485
NsiI ATGCAT 3 cut(s) 287, 1421, 1446
NspI RCATGY 2 cut(s) 1183, 1582
NspV TTCGAA 1 cut(s) 447
PaeI GCATGC 1 cut(s) 1582
PagI TCATGA 5 cut(s) 1108, 2368, 2751, 3216, 3583
PciSI GCTCTTC 3 cut(s) 510, 1110, 1723
PctI GAATGC 5 cut(s) 215, 1421, 1444, 2317, 3051
PdmI GAANNNNTTC 3 cut(s) 1252, 2019, 3195
PfeI GAWTC 5 cut(s) 1042, 1139, 1665, 1952, 2228
PflFI GACNNNGTC 1 cut(s) 3539
PflMI CCANNNNNTGG 2 cut(s) 556, 2136
PleI GAGTC 5 cut(s) 661, 850, 947, 2590, 2662
PpsI GAGTC 5 cut(s) 661, 850, 947, 2590, 2662
Ppu21I YACGTR 2 cut(s) 773, 3432
PshBI ATTAAT 1 cut(s) 2970
PsiI TTATAA 1 cut(s) 573
Psp124BI GAGCTC 2 cut(s) 913, 1105
Psp1406I AACGTT 1 cut(s) 1901
Psp6I CCWGG 4 cut(s) 426, 432, 1151, 3274
PspFI CCCAGC 1 cut(s) 2843
PspGI CCWGG 4 cut(s) 426, 432, 1151, 3274
PspN4I GGNNCC 6 cut(s) 114, 295, 829, 1634, 2289, 2565
PspPI GGNCC 7 cut(s) 430, 822, 1632, 2347, 2434, 2955, 3368
PstNI CAGNNNCTG 5 cut(s) 617, 1602, 2153, 2585, 2840
PsuI RGATCY 4 cut(s) 796, 1546, 2563, 2921
PsyI GACNNNGTC 1 cut(s) 3539
PvuII CAGCTG 3 cut(s) 1463, 2234, 2933
RsaI GTAC 6 cut(s) 90, 262, 932, 1307, 3403, 3613
RsaNI GTAC 6 cut(s) 89, 261, 931, 1306, 3402, 3612
RseI CAYNNNNRTG 3 cut(s) 323, 1449, 2342
SacI GAGCTC 2 cut(s) 913, 1105
SapI GCTCTTC 3 cut(s) 510, 1110, 1723
Sau96I GGNCC 7 cut(s) 430, 822, 1632, 2347, 2434, 2955, 3368
ScaI AGTACT 1 cut(s) 90
SchI GAGTC 5 cut(s) 662, 850, 947, 2591, 2663
ScrFI CCNGG 6 cut(s) 428, 434, 825, 1153, 1514, 3276
SduI GDGCHC 6 cut(s) 69, 427, 832, 913, 1105, 1428
SexAI ACCWGGT 1 cut(s) 3274
SfaNI GCATC 9 cut(s) 442, 495, 907, 1034, 1202, 1431, 1441, 2146, 2474
SfcI CTRYAG 3 cut(s) 1458, 1464, 2418
SfuI TTCGAA 1 cut(s) 447
SinI GGWCC 4 cut(s) 430, 1632, 2434, 2955
SmiMI CAYNNNNRTG 3 cut(s) 323, 1449, 2342
SmlI CTYRAG 4 cut(s) 341, 802, 1962, 2983
SmoI CTYRAG 4 cut(s) 341, 802, 1962, 2983
SpeI ACTAGT 1 cut(s) 1864
SphI GCATGC 1 cut(s) 1582
SsiI CCGC 4 cut(s) 1738, 2105, 2543, 3301
SspI AATATT 3 cut(s) 990, 1873, 2085
SstI GAGCTC 2 cut(s) 913, 1105
StyD4I CCNGG 6 cut(s) 426, 432, 823, 1151, 1512, 3274
StyI CCWWGG 3 cut(s) 1332, 3324, 3384
TaiI ACGT 5 cut(s) 125, 663, 775, 1904, 3434
TaqI TCGA 7 cut(s) 84, 447, 1015, 1929, 3349, 3376, 3624
TatI WGTACW 2 cut(s) 88, 1305
TfiI GAWTC 5 cut(s) 1042, 1139, 1665, 1952, 2228
TscAI CASTG 5 cut(s) 1443, 1603, 1855, 2331, 3568
TseFI GTSAC 3 cut(s) 468, 3086, 3520
Tsp45I GTSAC 3 cut(s) 468, 3086, 3520
TspGWI ACGGA 1 cut(s) 2647
TspRI CASTG 5 cut(s) 1443, 1603, 1855, 2331, 3568
Tth111I GACNNNGTC 1 cut(s) 3539
Van91I CCANNNNNTGG 2 cut(s) 556, 2136
VpaK11BI GGWCC 4 cut(s) 430, 1632, 2434, 2955
VspI ATTAAT 1 cut(s) 2970
XagI CCTNNNNNAGG 1 cut(s) 3317
XapI RAATTY 3 cut(s) 607, 1249, 2867
XbaI TCTAGA 1 cut(s) 16
XceI RCATGY 2 cut(s) 1183, 1582
XmiI GTMKAC 1 cut(s) 3558
XmnI GAANNNNTTC 3 cut(s) 1252, 2019, 3195
ZrmI AGTACT 1 cut(s) 90
Zsp2I ATGCAT 3 cut(s) 287, 1421, 1446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.