pycom15g31170

TLC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016678
Physical Location & Seq
Forward (+)
29494192 .. 29496232
2041 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 843 bp
ATGATGAGCAGGACAACGAGATGTCCGAAAGAGGACAAGGCGGGGCCCTTCTTCCTAGCCACTCTGGTGCTCTGGTTCGTCTCCGTTCTGTTCGAAATACTCTTCCACCGGCGCTCGGAGCTGCTCGCCGTTGTTGCCGGCTGCTTCTTTTACCAATTGGCCAACTGGGTTGTCCGCAGTTTCGTCTCCCGTGACCCTCTCTTCGTCAACACCTCCGTTTCGCTCCTCCACTCCTCCATAACCTCCGCTTCAGTGGTATTCATTTTGGTTAATCGGTCGTTAGAAAATGGCTCGGTTGGAATGTTTGAGCATGCGGAGTTGGTTGGACGTACTTGGCCATGGGCATACCCAGCTTTGTGCTTCTCATGCGGTTACTTTGCATATGATCAGTGGGATATGCTGCAGTATGGGTTATATAGCGGTTGGATTCCTTCCATCTTGATGCATCATCTGGTTCTCCTCATTTGTTTTACTCTTGCTTTGTATCGGAATGTCACCATCAATTACCTTATTCTCACTCTCATTTGTGAGCTGCACTCTATCTTTCTGCATGTGAGGAAAGTGCGGCGGATGGCAGGCATTCGTGACGCTACAAGCAAAATTGTAAAAGTAGAGTGGGTTCTTAATTGGGTCACCTTCACTCTAGCAAGGACGGCACCTCACATTCTCATCACAGCCAAGCTCTTCTGCGATGCTCCCAAATTCGGAAAGGGTATAGAGCTGCCAGTTGCGCTGTTTGGAATGTTCGGAATGAACTTTCTGAATGCTTCTCTTGGCATTGATCTCTTCCATGCTTTCAAGAGAGAGAGGAATCCTCAGCATGCTAATGCTCATCATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

281

Amino Acids

32.04

Weight (kDa)

9.12

Isoelectric Point (pI)

36.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 68 - 257 2e-19 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016678)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21790
fragaria_vesca FvH4_2g26990
malus_domestica MD08G1162300.v1.1 MD15G1347700.v1.1
prunus_persica Prupe.1G495500_v2.0.a1
pyrus_communis pycom15g31170
rosa_chinensis RchiOBHm_Chr6g0295871
rosa_laevigata RLG00000011761
rosa_multiflora Rmu_ssc0000277.1_g000001
rosa_roxburghii Rroxscaffold_7G00172100
rosa_rugosa Rorug06G0254900
rosa_samantha Rh6BG376200 Rh6CG382300
rosa_wichuraiana Rw6G032120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 655
AciI CCGC 8 cut(s) 41, 175, 246, 314, 369, 420, 565, 568
AcoI YGGCCR 2 cut(s) 159, 335
AcsI RAATTY 1 cut(s) 701
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 1 cut(s) 331
AfiI CCNNNNNNNGG 2 cut(s) 115, 704
AgsI TTSAA 1 cut(s) 799
AleI CACNNNNGTG 1 cut(s) 65
AluBI AGCT 5 cut(s) 121, 353, 532, 682, 721
AluI AGCT 5 cut(s) 121, 353, 532, 682, 721
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 2 cut(s) 85, 190
AoxI GGCC 3 cut(s) 44, 159, 335
ApaI GGGCCC 1 cut(s) 48
ApeKI GCWGC 5 cut(s) 121, 141, 400, 532, 721
ApoI RAATTY 1 cut(s) 701
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AspLEI GCGC 2 cut(s) 114, 733
AspS9I GGNCC 2 cut(s) 44, 45
AsuHPI GGTGA 2 cut(s) 487, 625
AsuII TTCGAA 1 cut(s) 93
BaeGI GKGCMC 1 cut(s) 48
BalI TGGCCA 2 cut(s) 161, 337
BanI GGYRCC 1 cut(s) 655
BanII GRGCYC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 72
BbvCI CCTCAGC 1 cut(s) 816
BbvI GCAGC 5 cut(s) 108, 128, 387, 519, 708
BccI CCATC 3 cut(s) 443, 506, 565
BceAI ACGGC 2 cut(s) 113, 669
BclI TGATCA 1 cut(s) 385
BcoDI GTCTC 2 cut(s) 85, 190
BfaI CTAG 2 cut(s) 56, 644
BfmI CTRYAG 1 cut(s) 401
BfoI RGCGCY 1 cut(s) 115
BisI GCNGC 6 cut(s) 122, 142, 401, 533, 566, 722
BlsI GCNGC 6 cut(s) 123, 143, 402, 534, 567, 723
BmgT120I GGNCC 2 cut(s) 44, 45
BmiI GGNNCC 3 cut(s) 45, 46, 657
BmrI ACTGGG 1 cut(s) 175
BmsI GCATC 3 cut(s) 432, 454, 682
BmuI ACTGGG 1 cut(s) 175
BplI GAGNNNNNCTC 4 cut(s) 521, 553, 799, 831
Bpu10I CCTNAGC 1 cut(s) 816
Bpu14I TTCGAA 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 338
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 704
Bse118I RCCGGY 2 cut(s) 108, 137
Bse1I ACTGG 2 cut(s) 170, 725
BseDI CCNNGG 1 cut(s) 338
BseGI GGATG 1 cut(s) 576
BseLI CCNNNNNNNGG 2 cut(s) 115, 704
BseMII CTCAG 1 cut(s) 830
BseNI ACTGG 2 cut(s) 170, 725
BseRI GAGGAG 3 cut(s) 215, 223, 449
BseSI GKGCMC 1 cut(s) 48
BseXI GCAGC 5 cut(s) 108, 128, 387, 519, 708
BseYI CCCAGC 1 cut(s) 349
BsgI GTGCAG 1 cut(s) 518
Bsh1285I CGRYCG 1 cut(s) 278
BshFI GGCC 3 cut(s) 46, 161, 337
BshNI GGYRCC 1 cut(s) 655
BsiEI CGRYCG 1 cut(s) 278
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 2 cut(s) 109, 138
BslI CCNNNNNNNGG 2 cut(s) 115, 704
BsmAI GTCTC 2 cut(s) 85, 190
BsmBI CGTCTC 2 cut(s) 85, 190
BsmI GAATGC 2 cut(s) 579, 769
BsnI GGCC 3 cut(s) 46, 161, 337
Bsp119I TTCGAA 1 cut(s) 93
Bsp120I GGGCCC 1 cut(s) 44
Bsp1286I GDGCHC 2 cut(s) 48, 72
Bsp143I GATC 2 cut(s) 385, 781
Bsp19I CCATGG 1 cut(s) 338
BspACI CCGC 8 cut(s) 41, 175, 246, 314, 369, 420, 565, 568
BspANI GGCC 3 cut(s) 46, 161, 337
BspCNI CTCAG 1 cut(s) 829
BspHI TCATGA 1 cut(s) 835
BspLI GGNNCC 3 cut(s) 45, 46, 657
BspMAI CTGCAG 1 cut(s) 405
BspQI GCTCTTC 1 cut(s) 689
BspT104I TTCGAA 1 cut(s) 93
BspT107I GGYRCC 1 cut(s) 655
BsrFI RCCGGY 2 cut(s) 108, 137
BsrI ACTGG 2 cut(s) 170, 725
BssAI RCCGGY 2 cut(s) 108, 137
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 2 cut(s) 385, 781
BssT1I CCWWGG 1 cut(s) 338
Bst6I CTCTTC 4 cut(s) 107, 206, 689, 791
BstBI TTCGAA 1 cut(s) 93
BstC8I GCNNGC 5 cut(s) 126, 139, 312, 577, 822
BstDEI CTNAG 1 cut(s) 816
BstDSI CCRYGG 1 cut(s) 338
BstEII GGTNACC 1 cut(s) 631
BstF5I GGATG 1 cut(s) 576
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 2 cut(s) 114, 733
BstKTI GATC 2 cut(s) 388, 784
BstMAI GTCTC 2 cut(s) 85, 190
BstMBI GATC 2 cut(s) 385, 781
BstMCI CGRYCG 1 cut(s) 278
BstMWI GCNNNNNNNGC 6 cut(s) 118, 134, 350, 366, 653, 730
BstNSI RCATGY 3 cut(s) 314, 554, 824
BstPI GGTNACC 1 cut(s) 631
BstSFI CTRYAG 1 cut(s) 401
BstSLI GKGCMC 1 cut(s) 48
BstV1I GCAGC 5 cut(s) 108, 128, 387, 519, 708
BsuRI GGCC 3 cut(s) 46, 161, 337
BtgI CCRYGG 1 cut(s) 338
BtgZI GCGATG 1 cut(s) 705
BtsCI GGATG 1 cut(s) 576
BtsIMutI CAGTG 2 cut(s) 258, 395
Cac8I GCNNGC 5 cut(s) 126, 139, 312, 577, 822
CciI TCATGA 1 cut(s) 835
CfoI GCGC 2 cut(s) 114, 733
Cfr10I RCCGGY 2 cut(s) 108, 137
Cfr13I GGNCC 2 cut(s) 44, 45
CseI GACGC 1 cut(s) 596
Csp6I GTAC 1 cut(s) 330
CviAII CATG 7 cut(s) 311, 339, 366, 551, 791, 821, 836
CviQI GTAC 1 cut(s) 330
DdeI CTNAG 1 cut(s) 816
DpnI GATC 2 cut(s) 387, 783
DpnII GATC 2 cut(s) 385, 781
EaeI YGGCCR 2 cut(s) 159, 335
Eam1104I CTCTTC 4 cut(s) 107, 206, 689, 791
EarI CTCTTC 4 cut(s) 107, 206, 689, 791
EciI GGCGGA 1 cut(s) 583
Eco130I CCWWGG 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 234
Eco91I GGTNACC 1 cut(s) 631
EcoO109I RGGNCCY 2 cut(s) 44, 45
EcoO65I GGTNACC 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 338
EcoT22I ATGCAT 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 338
Esp3I CGTCTC 2 cut(s) 85, 190
FaeI CATG 7 cut(s) 314, 342, 369, 554, 794, 824, 839
FatI CATG 7 cut(s) 310, 338, 365, 550, 790, 820, 835
FauI CCCGC 1 cut(s) 34
FauNDI CATATG 1 cut(s) 382
FbaI TGATCA 1 cut(s) 385
Fnu4HI GCNGC 6 cut(s) 122, 142, 401, 533, 566, 722
FokI GGATG 1 cut(s) 583
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 6 cut(s) 122, 142, 401, 533, 566, 722
FspBI CTAG 2 cut(s) 56, 644
GlaI GCGC 2 cut(s) 113, 732
GluI GCNGC 6 cut(s) 122, 142, 401, 533, 566, 722
GsaI CCCAGC 1 cut(s) 353
HaeII RGCGCY 1 cut(s) 115
HaeIII GGCC 3 cut(s) 46, 161, 337
HapII CCGG 2 cut(s) 109, 138
HgaI GACGC 1 cut(s) 596
HhaI GCGC 2 cut(s) 114, 733
Hin1II CATG 7 cut(s) 314, 342, 369, 554, 794, 824, 839
Hin6I GCGC 2 cut(s) 112, 731
HinP1I GCGC 2 cut(s) 112, 731
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 2 cut(s) 427, 811
HpaII CCGG 2 cut(s) 109, 138
HphI GGTGA 2 cut(s) 487, 625
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 6 cut(s) 27, 118, 489, 707, 749, 762
Hpy188III TCNNGA 4 cut(s) 439, 584, 799, 836
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 3 cut(s) 58, 441, 646
HpyCH4IV ACGT 1 cut(s) 328
HpyCH4V TGCA 5 cut(s) 380, 403, 445, 535, 550
HpyF10VI GCNNNNNNNGC 6 cut(s) 118, 134, 350, 366, 653, 730
HpyF3I CTNAG 1 cut(s) 816
HpySE526I ACGT 1 cut(s) 328
Hsp92II CATG 7 cut(s) 314, 342, 369, 554, 794, 824, 839
HspAI GCGC 2 cut(s) 112, 731
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 2 cut(s) 385, 781
LguI GCTCTTC 1 cut(s) 689
LmnI GCTCC 3 cut(s) 118, 228, 700
LpnPI CCDG 9 cut(s) 50, 58, 122, 151, 151, 363, 437, 561, 738
Lsp1109I GCAGC 5 cut(s) 108, 128, 387, 519, 708
LweI GCATC 3 cut(s) 432, 454, 682
MaeI CTAG 2 cut(s) 56, 644
MaeII ACGT 1 cut(s) 328
MaeIII GTNAC 5 cut(s) 191, 371, 493, 584, 631
MalI GATC 2 cut(s) 387, 783
MboI GATC 2 cut(s) 385, 781
MboII GAAGA 5 cut(s) 43, 94, 193, 676, 778
MfeI CAATTG 1 cut(s) 155
MhlI GDGCHC 2 cut(s) 48, 72
MlsI TGGCCA 2 cut(s) 161, 337
MluCI AATT 5 cut(s) 155, 502, 600, 625, 701
MluNI TGGCCA 2 cut(s) 161, 337
MmeI TCCRAC 3 cut(s) 277, 304, 404
Mox20I TGGCCA 2 cut(s) 161, 337
Mph1103I ATGCAT 1 cut(s) 447
MroNI GCCGGC 1 cut(s) 137
MscI TGGCCA 2 cut(s) 161, 337
MseI TTAA 2 cut(s) 270, 624
MslI CAYNNNNRTG 3 cut(s) 65, 440, 825
Msp20I TGGCCA 2 cut(s) 161, 337
MspI CCGG 2 cut(s) 109, 138
MunI CAATTG 1 cut(s) 155
Mva1269I GAATGC 2 cut(s) 579, 769
MwoI GCNNNNNNNGC 6 cut(s) 118, 134, 350, 366, 653, 730
NaeI GCCGGC 1 cut(s) 139
NcoI CCATGG 1 cut(s) 338
NdeI CATATG 1 cut(s) 382
NdeII GATC 2 cut(s) 385, 781
NgoMIV GCCGGC 1 cut(s) 137
NlaIII CATG 7 cut(s) 314, 342, 369, 554, 794, 824, 839
NlaIV GGNNCC 3 cut(s) 45, 46, 657
NmuCI GTSAC 4 cut(s) 191, 493, 584, 631
NsiI ATGCAT 1 cut(s) 447
NspI RCATGY 3 cut(s) 314, 554, 824
NspV TTCGAA 1 cut(s) 93
OliI CACNNNNGTG 1 cut(s) 65
PaeI GCATGC 2 cut(s) 314, 824
PagI TCATGA 1 cut(s) 835
PciSI GCTCTTC 1 cut(s) 689
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 2 cut(s) 579, 769
PdiI GCCGGC 1 cut(s) 139
PfeI GAWTC 2 cut(s) 427, 811
PkrI GCNGC 6 cut(s) 123, 143, 402, 534, 567, 723
PspEI GGTNACC 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 349
PspN4I GGNNCC 3 cut(s) 45, 46, 657
PspOMI GGGCCC 1 cut(s) 44
PspPI GGNCC 2 cut(s) 44, 45
PsrI GAACNNNNNNTAC 2 cut(s) 603, 635
PstI CTGCAG 1 cut(s) 405
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
RseI CAYNNNNRTG 3 cut(s) 65, 440, 825
SapI GCTCTTC 1 cut(s) 689
SaqAI TTAA 2 cut(s) 270, 624
SatI GCNGC 6 cut(s) 122, 142, 401, 533, 566, 722
Sau3AI GATC 2 cut(s) 385, 781
Sau96I GGNCC 2 cut(s) 44, 45
SduI GDGCHC 2 cut(s) 48, 72
SfaNI GCATC 3 cut(s) 432, 454, 682
SfcI CTRYAG 1 cut(s) 401
SfuI TTCGAA 1 cut(s) 93
SgrAI CRCCGGYG 1 cut(s) 108
SmiMI CAYNNNNRTG 3 cut(s) 65, 440, 825
SphI GCATGC 2 cut(s) 314, 824
Sse9I AATT 5 cut(s) 155, 502, 600, 625, 701
SsiI CCGC 8 cut(s) 41, 175, 246, 314, 369, 420, 565, 568
SspMI CTAG 2 cut(s) 56, 644
StyI CCWWGG 1 cut(s) 338
TaiI ACGT 1 cut(s) 331
TaqI TCGA 1 cut(s) 93
TaqII GACCGA 1 cut(s) 264
TasI AATT 5 cut(s) 155, 502, 600, 625, 701
TauI GCSGC 1 cut(s) 568
TfiI GAWTC 2 cut(s) 427, 811
Tru1I TTAA 2 cut(s) 270, 624
Tru9I TTAA 2 cut(s) 270, 624
TscAI CASTG 2 cut(s) 258, 395
TseFI GTSAC 4 cut(s) 191, 493, 584, 631
TseI GCWGC 5 cut(s) 121, 141, 400, 532, 721
Tsp45I GTSAC 4 cut(s) 191, 493, 584, 631
TspDTI ATGAA 2 cut(s) 250, 767
TspGWI ACGGA 2 cut(s) 73, 205
TspRI CASTG 2 cut(s) 258, 395
XapI RAATTY 1 cut(s) 701
XceI RCATGY 3 cut(s) 314, 554, 824
XspI CTAG 2 cut(s) 56, 644
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.