pycom15g31310

Sister chromatid cohesion protein PDS5 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003552
Physical Location & Seq
Reverse (-)
29677435 .. 29693481
16047 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4029 bp
ATGGCCGAGTCCGCGCTGCAACTCGTCTCGGAAATCGGCACCCACCTCCGCCGCCAAACGCGCCCAAACAAGGACTTCATCGTCAAATCTCTCCGACAAGCTGCAAGTGCTTTGTCGCAGTTAGAACAGGCTTCTTCGGCTGAAGCTTGTAAGAAGTTGGAGCCCCTGAGAGAAGCTATTGTGCATGGCTTGCTTCAGCATAGGGATAAGGACGTCAGGCTTCTTGTGGCCATCTGTGTAACTGAAATGTTTCGGGCTATGGCACCCGAACCCCCTTTTGCGGACAAATATCTAAGGAACATTTTTAAACTCATTATCAGCACGTTCGTCGAGCTAGCTGATATGGAGAGCCCGCTCTTTTCGAGGAGAGCCAAAATAGTAGAGACTGTCGCGCGATGTAAATGTTGTGTGATCATGTTGGATGTTGACTGCAATGATCTAGTTCTCGAAATGTTCAATGTCTTCTTCTCTGTTGTGAGGCAACATCATCAGCAGAGTTTGATGAATGATATTTTGTCTATAATGGTTCATATACTAAACGAGGAAGCTTCTCAGCCACTTTTGGAAGTGGTTCTACGAAATCTTGTGAAGGAGGCAAAGGATGCAGATTCTGCTTCTTCTCAGCTTGCAGTTTCTGTGATCCAAACATGCGCAGACAAACTTGAGTCCTTTGTTTGTGGGTTTCTGACATCTTGTATTTTGGATGGAGATGCTGATGGGAGTGAGCTCAAGGAATTTTACCATGAAATCATTTTTAAAATTTTTGGGTGTGCTCCTCAGATGCTTCTTGCTGTCATGCCAAATTTGACTCAAGAGTTACTGACTGATCAGGTTGATGTCCGACTAAAAGCCGTTAATTTAATTGGAAAACTTTTCACACTGCCTGATCACCATATTGCACAAAGGTATCATGACCTCTTTGTTGAGTTTTTGAAAAGATTTTCGGATAAATCTGCGGATGTTAGAGTTAGTGCTCTGCAATGTGCTAAAGTTTGCTACATGACCAACCCCTCTGGGGCAGAATCACAAGAAATTCTCCCTGCCCTTGAAAGTCGGCTACTAGATTTTGATGATAGGGTGAGAACACAGGCAGTGATTGTTGCCTGTGATCTTGCCATGTCTAATATGAGATGCTTTCCTCCCAAAATAATATCTCAGACCACTGAAAGACTTAGGGATAAGAAGATACCCGTTAGAAAGAAAGCTTTGCAGAAGTTGATGGAAGTATATCGAGATTATTGTAACAAATGCTTTAAAGGCTCTATGACAATCAGTGACCACTTTGAACAGATTCCTTGTAAAATATTGATGCTATGCTTTGATAAAGATTGTATGGATTTCAGGTCCCAAAATATGGAGCTTGTTCTTGCAGAAGATCTATTTCCAGCTGGTCTTTCAGTTCATGAAATTACAAGGCACTGGATCCACTTGTTTTCTCTTTTCACCCCACTTCATATAAAGGCTTTGAACTCTATTTTATCCCAGAAACAAAGGTTGCAAAGTGAGATGCAGACTTATTTAGCCAATCGAAAGAAAGAGAAGGGGAGTAATTCAGAAGAGATGCAGAAGAGATATAAAGTTCAGTTCTCAAAAATGGCAGTCTCCTTTGCAGACCCTTCAAGAGCAGAAGAGTGCTTTGAAAAATTTAACCAAATGAAAGATAATAACATTTTTAATTCACTGGCTCTGTTATTGGATGACCTGAAATTTAAAGAAGCCCGTGCAAGCAGAGACAAATTTCTGAATATGATTGGCGGGAAACATCAGAATTTTGAGTTTTTACGAACACTTTCCTCAAAATGCTCTTACAATATATTCAGTGCAGCGCATGTTCGCTGTATTCTAGATGATCTATCCGGTAACAGTCCTGGAAAGAGAAACTTGGAGGCTTCTTCTGTCAGACTTCTGCTGTCAATCACCAGCTTTTTCCCAACTCTCTTAAGAGGTTCAGAAGTCCAACTTCAGAAGTTATTAAAGGAGACGGACCCAATTAATGATAAGTTGATTGAAGTGTTAGCCAAAGCAGGCCCTCAAATATTTGTTAAACTCAGTGAAATCTACCCCTTTCTGAAGAGGGTTTGTCTGGAGGGGACTCGCGTTCAGTCAAAATATGCTGTTTCAGCAATTGCTGCTTTAGCTGATCATTCAAAGCAATTGATTTTCCAAGATCTATGTAAGGAACTTGTGGATTCTCTACTAGTTGGGCAGAACATACCAACAGTGTTACAGTCCTTGGGGTGTCTTGCACAGCATTCTGTTTCAACATTCCAAAGCCAAGTTGGAGAGATCACCCATTATATATATCAAAAGATATTCCAAGTGAACTCTTCAGATTTTATAGACTCCTGTGATGATGCGTCTGGGTCTAGTGATTCTTGCAAATTGAAGATATATGGCCTGAAAACACTTGTCAAGAGCTTCTTGCCACACAGGGGAACTCACACCAAACAGCAAATTAATGAGCTTGAGGACAACACCAAACGGCAAATTGATGAGCTCTGGGACATTTTGTCAACAATGCTGCAAAAGGGAGAAACTGCTGAGGGTATCACCTCATGTGAAAGTGATAAGGCTTGTATTAGATTAGCTGCTGCAAAGTCTGTTCTCCGGCTTGCTCGAAGATGGGATTTCCATATTTCTCCGGAGATCTTTCACTTCACAATTTTAATGGCAAAGGATGATTCTCCATTGGTGAGAAGATCATTTCTTGATAAAACTCACAAATTACTGAAGGAGCATGTTATACCTAGCAGATATGCATGTGCTTTCGCAATGGCCACTTCAGATTGCCTCAAGGATCTGCAGGATGATTCGTTAAAATATATTGCAGAATTTGTCAAGGATTACAGTAGAGAAGCTCAAGTGCGTCAAATCTCTGGAGTCCAAGAAGGATTAAACACTGATTTTCCAGCATACATAGTGGTGTTCTTGATCCATATTCTTGCTCATGATACTGGCTTCCCACCTGAAGACTGTCAAGATGAAAAAGCATATGCTCAGTTTTGCGGTCCACTGTTAGCTTTATTGCAGGCTTTAGTCAATGCTAGTAATGCTGATGGGGCTCTGGATGTTGCCAAGGATTCTGTCTTGTATTTGATTTGCATTTTTCGTGCAATTAAGAGAGCTGAGGATGCTATAGACACTGAATTAACCGGCAAGCTGCATATTCTGGCAGAAATTGGGCATGCTTTTGTGACGTTAACAAATCATAATGGCCTCTCCTTATTACATGCTCCTGGGAAAATATTTCTACCATCATCCTTATATAAATCCAATTCAAGATGCCTCACTCAATCATGTTTTGATGAGTACTTTTTCAAAAGAGTAGTTGACATATTTAAATCAAATATCTCTTTGCCTGCCAGTGCTCTTCCTAGACGTGGGCGTAAATGTCAAGAGGATATCACACAATCTGGTGTTGTCAAGGAGAGCAAACAAATCGTAACATCTTCCAAGATAGTTAATTTGTGCAATGATGGGGCAGCTGAACCCCAAAAGGCTGTGAAACAAGGCACCAGTACAGGAGGATGTAGAAGAAAACGAGATCTCTCTCCAAGTGATTCTGATATTGGTTGCCAGAATGGTTTATCTAAAAAATCTGAGATTACTTTGGAAAAAGAAATACTTTCATCTTGTGATTCTGTGGCTACAGTCGCTGGATCAAATGCCACTGTCCAGAATATTAAAAAAAATACCATTCCATTGATGGGAAATGTTAATGTGAAAAGAAGCATTAATGTGGAACATTCCAATGACCCCAGATCAAATCTAAAGGGTCCTTGCAGCTTAAAGGAAATTAGTAAGAAGGCTGAGGCATTGATTGGGCAAAGAATCAAATTTTTGTCTCCTGTAGATAAATGTTTTTATTCAGGTACAGTGGATGGTTATAATGCTCAAAACAATACATACAAGAAACGAAAGCTGGGGAAAAAAGCTTTTGTGGACACCTCAGCATCAGAAGTTACTGATGCAAATGAGGATGCTGTGGCTAGAAGAACTCGAAGACAAAAGAAACTAAATGAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1343

Amino Acids

150.7

Weight (kDa)

8.41

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDS5 PF20168 26 - 1090 2.3e-284 Sister chromatid cohesion protein PDS5 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3888
AasI GACNNNNNNGTC 1 cut(s) 80
AatII GACGTC 1 cut(s) 216
Acc16I TGCGCA 1 cut(s) 652
AccB1I GGYRCC 3 cut(s) 38, 262, 3512
AccB7I CCANNNNNTGG 1 cut(s) 1354
AccBSI CCGCTC 1 cut(s) 355
AccII CGCG 5 cut(s) 14, 61, 392, 394, 2097
AccIII TCCGGA 1 cut(s) 2641
AciI CCGC 8 cut(s) 12, 49, 52, 281, 353, 956, 1755, 3006
AclWI GGATC 6 cut(s) 634, 1417, 1430, 2805, 2926, 3667
AcoI YGGCCR 3 cut(s) 3, 228, 2775
AcuI CTGAAG 8 cut(s) 162, 179, 1946, 2090, 2313, 2750, 2766, 2988
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 3 cut(s) 3311, 3520, 3874
AfiI CCNNNNNNNGG 7 cut(s) 70, 280, 1015, 1354, 2432, 3380, 3707
AflII CTTAAG 1 cut(s) 1939
AhdI GACNNNNNGTC 1 cut(s) 2509
AhlI ACTAGT 1 cut(s) 2197
AjiI CACGTC 1 cut(s) 3380
AjnI CCWGG 2 cut(s) 1867, 3235
Alw21I GWGCWC 5 cut(s) 729, 775, 976, 2499, 3370
Alw26I GTCTC 6 cut(s) 31, 377, 1606, 1725, 1973, 3849
AlwI GGATC 6 cut(s) 634, 1417, 1430, 2805, 2926, 3667
AlwNI CAGNNNCTG 3 cut(s) 611, 635, 3656
Aor13HI TCCGGA 1 cut(s) 2641
AoxI GGCC 6 cut(s) 3, 228, 2026, 2395, 2775, 3214
AseI ATTAAT 3 cut(s) 1992, 2457, 3735
Asp700I GAANNNNTTC 4 cut(s) 249, 570, 1290, 1789
AspLEI GCGC 5 cut(s) 16, 63, 394, 653, 1828
AspS9I GGNCC 5 cut(s) 1344, 1984, 2027, 3008, 3776
AsuHPI GGTGA 7 cut(s) 881, 1090, 1435, 1909, 2281, 2542, 2704
AsuNHI GCTAGC 1 cut(s) 334
AvaII GGWCC 4 cut(s) 1344, 1984, 3008, 3776
BaeI ACNNNNGTAYC 2 cut(s) 890, 923
BalI TGGCCA 2 cut(s) 230, 2777
BamHI GGATCC 1 cut(s) 1422
BanI GGYRCC 3 cut(s) 38, 262, 3512
BanII GRGCYC 5 cut(s) 165, 353, 729, 2499, 3064
BarI GAAGNNNNNNTAC 2 cut(s) 558, 590
BbsI GAAGAC 3 cut(s) 454, 2976, 4009
Bbv12I GWGCWC 5 cut(s) 729, 775, 976, 2499, 3370
BbvCI CCTCAGC 4 cut(s) 2541, 3126, 3810, 3949
BceAI ACGGC 2 cut(s) 836, 2498
BcgI CGANNNNNNTGC 4 cut(s) 310, 344, 3421, 3455
BciT130I CCWGG 2 cut(s) 1869, 3237
BclI TGATCA 4 cut(s) 411, 826, 886, 2140
BcoDI GTCTC 6 cut(s) 31, 377, 1606, 1725, 1973, 3849
BcuI ACTAGT 1 cut(s) 2197
BfmI CTRYAG 4 cut(s) 2801, 3135, 3648, 3849
BfrI CTTAAG 1 cut(s) 1939
BglII AGATCT 4 cut(s) 1375, 2167, 2646, 3544
BmcAI AGTACT 1 cut(s) 3311
Bme1390I CCNGG 2 cut(s) 1869, 3237
Bme18I GGWCC 4 cut(s) 1344, 1984, 3008, 3776
BmeRI GACNNNNNGTC 1 cut(s) 2509
BmgBI CACGTC 1 cut(s) 3380
BmgT120I GGNCC 5 cut(s) 1344, 1984, 2027, 3008, 3776
BmiI GGNNCC 8 cut(s) 40, 162, 264, 1346, 1424, 1986, 3514, 3777
BmrFI CCNGG 2 cut(s) 1869, 3237
BmtI GCTAGC 1 cut(s) 338
BpiI GAAGAC 3 cut(s) 454, 2976, 4009
BpmI CTGGAG 2 cut(s) 2105, 2898
Bpu10I CCTNAGC 4 cut(s) 2541, 3126, 3810, 3949
BpuEI CTTGAG 6 cut(s) 683, 713, 795, 2486, 2777, 2844
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 3 cut(s) 2232, 3075, 3236
BsaWI WCCGGW 2 cut(s) 1856, 2641
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 280, 1015, 1354, 2432, 3380, 3707
Bse118I RCCGGY 1 cut(s) 3152
Bse1I ACTGG 5 cut(s) 1424, 1686, 2959, 3363, 3516
Bse3DI GCAATG 4 cut(s) 439, 986, 2778, 3478
BseAI TCCGGA 1 cut(s) 2641
BseBI CCWGG 2 cut(s) 1869, 3237
BseDI CCNNGG 3 cut(s) 2232, 3075, 3236
BseLI CCNNNNNNNGG 7 cut(s) 70, 280, 1015, 1354, 2432, 3380, 3707
BseMI GCAATG 4 cut(s) 439, 986, 2778, 3478
BseNI ACTGG 5 cut(s) 1424, 1686, 2959, 3363, 3516
BseRI GAGGAG 2 cut(s) 379, 765
BseYI CCCAGC 1 cut(s) 3922
BsgI GTGCAG 1 cut(s) 1842
Bsh1236I CGCG 5 cut(s) 14, 61, 392, 394, 2097
BshFI GGCC 6 cut(s) 5, 230, 2028, 2397, 2777, 3216
BshNI GGYRCC 3 cut(s) 38, 262, 3512
BsiHKAI GWGCWC 5 cut(s) 729, 775, 976, 2499, 3370
BsiSI CCGG 4 cut(s) 1857, 2608, 2642, 3153
BslFI GGGAC 3 cut(s) 1330, 2104, 2516
BslI CCNNNNNNNGG 7 cut(s) 70, 280, 1015, 1354, 2432, 3380, 3707
BsmAI GTCTC 6 cut(s) 31, 377, 1606, 1725, 1973, 3849
BsmBI CGTCTC 2 cut(s) 31, 1973
BsmFI GGGAC 3 cut(s) 1330, 2104, 2516
BsmI GAATGC 1 cut(s) 2251
BsnI GGCC 6 cut(s) 5, 230, 2028, 2397, 2777, 3216
Bsp1286I GDGCHC 8 cut(s) 165, 353, 729, 775, 976, 2499, 3064, 3370
Bsp13I TCCGGA 1 cut(s) 2641
BspACI CCGC 8 cut(s) 12, 49, 52, 281, 353, 956, 1755, 3006
BspANI GGCC 6 cut(s) 5, 230, 2028, 2397, 2777, 3216
BspEI TCCGGA 1 cut(s) 2641
BspFNI CGCG 5 cut(s) 14, 61, 392, 394, 2097
BspHI TCATGA 3 cut(s) 910, 1402, 2947
BspLI GGNNCC 8 cut(s) 40, 162, 264, 1346, 1424, 1986, 3514, 3777
BspMAI CTGCAG 1 cut(s) 2805
BspOI GCTAGC 1 cut(s) 338
BspPI GGATC 6 cut(s) 634, 1417, 1430, 2805, 2926, 3667
BspQI GCTCTTC 1 cut(s) 3375
BspT107I GGYRCC 3 cut(s) 38, 262, 3512
BspTI CTTAAG 1 cut(s) 1939
BsrBI CCGCTC 1 cut(s) 355
BsrDI GCAATG 4 cut(s) 439, 986, 2778, 3478
BsrFI RCCGGY 1 cut(s) 3152
BsrI ACTGG 5 cut(s) 1424, 1686, 2959, 3363, 3516
BssAI RCCGGY 1 cut(s) 3152
BssECI CCNNGG 3 cut(s) 2232, 3075, 3236
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 2 cut(s) 2232, 3075
Bst2UI CCWGG 2 cut(s) 1869, 3237
Bst6I CTCTTC 6 cut(s) 1551, 1562, 1623, 2066, 2332, 3375
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 1939
BstAPI GCANNNNNTGC 4 cut(s) 190, 602, 611, 2129
BstFNI CGCG 5 cut(s) 14, 61, 392, 394, 2097
BstHHI GCGC 5 cut(s) 16, 63, 394, 653, 1828
BstMAI GTCTC 6 cut(s) 31, 377, 1606, 1725, 1973, 3849
BstNI CCWGG 2 cut(s) 1869, 3237
BstNSI RCATGY 6 cut(s) 651, 1832, 2741, 2763, 3188, 3233
BstSCI CCNGG 2 cut(s) 1867, 3235
BstSFI CTRYAG 4 cut(s) 2801, 3135, 3648, 3849
BstUI CGCG 5 cut(s) 14, 61, 392, 394, 2097
BstV2I GAAGAC 3 cut(s) 454, 2976, 4009
BstX2I RGATCY 6 cut(s) 1375, 1422, 2167, 2646, 2797, 3544
BstYI RGATCY 6 cut(s) 1375, 1422, 2167, 2646, 2797, 3544
BsuRI GGCC 6 cut(s) 5, 230, 2028, 2397, 2777, 3216
BtgZI GCGATG 1 cut(s) 409
BtrI CACGTC 1 cut(s) 3380
BtsI GCAGTG 2 cut(s) 878, 1098
CaiI CAGNNNCTG 3 cut(s) 611, 635, 3656
CciI TCATGA 3 cut(s) 910, 1402, 2947
CfoI GCGC 5 cut(s) 16, 63, 394, 653, 1828
Cfr10I RCCGGY 1 cut(s) 3152
Cfr13I GGNCC 5 cut(s) 1344, 1984, 2027, 3008, 3776
CseI GACGC 2 cut(s) 2346, 2855
Csp6I GTAC 3 cut(s) 3310, 3519, 3873
CviQI GTAC 3 cut(s) 3310, 3519, 3873
DraI TTTAAA 5 cut(s) 307, 757, 1255, 1711, 3340
DrdI GACNNNNNNGTC 1 cut(s) 80
DriI GACNNNNNGTC 1 cut(s) 2509
DseDI GACNNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 3 cut(s) 3, 228, 2775
Eam1104I CTCTTC 6 cut(s) 1551, 1562, 1623, 2066, 2332, 3375
Eam1105I GACNNNNNGTC 1 cut(s) 2509
EarI CTCTTC 6 cut(s) 1551, 1562, 1623, 2066, 2332, 3375
EciI GGCGGA 1 cut(s) 38
Ecl136II GAGCTC 2 cut(s) 727, 2497
Eco130I CCWWGG 2 cut(s) 2232, 3075
Eco24I GRGCYC 5 cut(s) 165, 353, 729, 2499, 3064
Eco32I GATATC 1 cut(s) 3403
Eco47I GGWCC 4 cut(s) 1344, 1984, 3008, 3776
Eco53kI GAGCTC 2 cut(s) 727, 2497
Eco57I CTGAAG 8 cut(s) 162, 179, 1946, 2090, 2313, 2750, 2766, 2988
EcoICRI GAGCTC 2 cut(s) 727, 2497
EcoO109I RGGNCCY 3 cut(s) 1344, 2027, 3776
EcoRII CCWGG 2 cut(s) 1867, 3235
EcoRV GATATC 1 cut(s) 3403
EcoT14I CCWWGG 2 cut(s) 2232, 3075
EcoT22I ATGCAT 1 cut(s) 2761
EcoT38I GRGCYC 5 cut(s) 165, 353, 729, 2499, 3064
ErhI CCWWGG 2 cut(s) 2232, 3075
Esp3I CGTCTC 2 cut(s) 31, 1973
FalI AAGNNNNNCTT 8 cut(s) 1865, 1897, 1944, 1976, 2405, 2437, 3609, 3641
FaqI GGGAC 3 cut(s) 1330, 2104, 2516
FauI CCCGC 2 cut(s) 360, 1748
FauNDI CATATG 1 cut(s) 2992
FbaI TGATCA 4 cut(s) 411, 826, 886, 2140
FriOI GRGCYC 5 cut(s) 165, 353, 729, 2499, 3064
FspI TGCGCA 1 cut(s) 652
GlaI GCGC 5 cut(s) 15, 62, 393, 652, 1827
GsaI CCCAGC 1 cut(s) 3926
GsuI CTGGAG 2 cut(s) 2105, 2898
HaeIII GGCC 6 cut(s) 5, 230, 2028, 2397, 2777, 3216
HapII CCGG 4 cut(s) 1857, 2608, 2642, 3153
HgaI GACGC 2 cut(s) 2346, 2855
HhaI GCGC 5 cut(s) 16, 63, 394, 653, 1828
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 5 cut(s) 14, 61, 392, 651, 1826
HinP1I GCGC 5 cut(s) 14, 61, 392, 651, 1826
HincII GTYRAC 4 cut(s) 427, 2514, 3201, 3331
HindII GTYRAC 4 cut(s) 427, 2514, 3201, 3331
HindIII AAGCTT 4 cut(s) 144, 546, 1203, 3933
HpaI GTTAAC 1 cut(s) 3201
HpaII CCGG 4 cut(s) 1857, 2608, 2642, 3153
HphI GGTGA 7 cut(s) 881, 1090, 1435, 1909, 2281, 2542, 2704
Hpy166II GTNNAC 7 cut(s) 427, 2323, 2514, 3011, 3201, 3331, 3943
Hpy8I GTNNAC 7 cut(s) 427, 2323, 2514, 3011, 3201, 3331, 3943
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 6 cut(s) 583, 1534, 1626, 2725, 2882, 3799
HpyCH4IV ACGT 4 cut(s) 213, 323, 3197, 3379
HpySE526I ACGT 4 cut(s) 213, 323, 3197, 3379
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 5 cut(s) 14, 61, 392, 651, 1826
Kpn2I TCCGGA 1 cut(s) 2641
Ksp22I TGATCA 4 cut(s) 411, 826, 886, 2140
KspAI GTTAAC 1 cut(s) 3201
LguI GCTCTTC 1 cut(s) 3375
LmnI GCTCC 5 cut(s) 160, 778, 1357, 2734, 3238
MaeII ACGT 4 cut(s) 213, 323, 3197, 3379
MaeIII GTNAC 9 cut(s) 238, 816, 1241, 1274, 1859, 2223, 3193, 3442, 3961
MbiI CCGCTC 1 cut(s) 355
MfeI CAATTG 2 cut(s) 2124, 2153
MflI RGATCY 6 cut(s) 1375, 1422, 2167, 2646, 2797, 3544
MhlI GDGCHC 8 cut(s) 165, 353, 729, 775, 976, 2499, 3064, 3370
MlsI TGGCCA 2 cut(s) 230, 2777
MluNI TGGCCA 2 cut(s) 230, 2777
MlyI GAGTC 6 cut(s) 17, 674, 802, 2086, 2336, 2889
MmeI TCCRAC 6 cut(s) 118, 138, 399, 865, 1981, 2260
Mox20I TGGCCA 2 cut(s) 230, 2777
Mph1103I ATGCAT 1 cut(s) 2761
MroI TCCGGA 1 cut(s) 2641
MroXI GAANNNNTTC 4 cut(s) 249, 570, 1290, 1789
MscI TGGCCA 2 cut(s) 230, 2777
MslI CAYNNNNRTG 3 cut(s) 2921, 3737, 3750
Msp20I TGGCCA 2 cut(s) 230, 2777
MspA1I CMGCKG 2 cut(s) 1388, 3485
MspCI CTTAAG 1 cut(s) 1939
MspI CCGG 4 cut(s) 1857, 2608, 2642, 3153
MspR9I CCNGG 2 cut(s) 1869, 3237
MunI CAATTG 2 cut(s) 2124, 2153
Mva1269I GAATGC 1 cut(s) 2251
MvaI CCWGG 2 cut(s) 1869, 3237
MvnI CGCG 5 cut(s) 14, 61, 392, 394, 2097
NdeI CATATG 1 cut(s) 2992
NheI GCTAGC 1 cut(s) 334
NlaIV GGNNCC 8 cut(s) 40, 162, 264, 1346, 1424, 1986, 3514, 3777
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 2 cut(s) 1274, 3193
NsbI TGCGCA 1 cut(s) 652
NsiI ATGCAT 1 cut(s) 2761
NspI RCATGY 6 cut(s) 651, 1832, 2741, 2763, 3188, 3233
PaeI GCATGC 1 cut(s) 3188
PagI TCATGA 3 cut(s) 910, 1402, 2947
PciSI GCTCTTC 1 cut(s) 3375
PctI GAATGC 1 cut(s) 2251
PdmI GAANNNNTTC 4 cut(s) 249, 570, 1290, 1789
PflMI CCANNNNNTGG 1 cut(s) 1354
PfoI TCCNGGA 1 cut(s) 1867
PleI GAGTC 6 cut(s) 16, 673, 802, 2086, 2336, 2888
PpsI GAGTC 6 cut(s) 16, 673, 802, 2086, 2336, 2888
PpuMI RGGWCCY 2 cut(s) 1344, 3776
PshBI ATTAAT 3 cut(s) 1992, 2457, 3735
PsiI TTATAA 1 cut(s) 3888
Psp124BI GAGCTC 2 cut(s) 729, 2499
Psp5II RGGWCCY 2 cut(s) 1344, 3776
Psp6I CCWGG 2 cut(s) 1867, 3235
PspFI CCCAGC 1 cut(s) 3922
PspGI CCWGG 2 cut(s) 1867, 3235
PspN4I GGNNCC 8 cut(s) 40, 162, 264, 1346, 1424, 1986, 3514, 3777
PspPI GGNCC 5 cut(s) 1344, 1984, 2027, 3008, 3776
PspPPI RGGWCCY 2 cut(s) 1344, 3776
PstI CTGCAG 1 cut(s) 2805
PstNI CAGNNNCTG 3 cut(s) 611, 635, 3656
PsuI RGATCY 6 cut(s) 1375, 1422, 2167, 2646, 2797, 3544
PvuII CAGCTG 2 cut(s) 1388, 3485
RsaI GTAC 3 cut(s) 3311, 3520, 3874
RsaNI GTAC 3 cut(s) 3310, 3519, 3873
RseI CAYNNNNRTG 3 cut(s) 2921, 3737, 3750
SacI GAGCTC 2 cut(s) 729, 2499
SapI GCTCTTC 1 cut(s) 3375
Sau96I GGNCC 5 cut(s) 1344, 1984, 2027, 3008, 3776
ScaI AGTACT 1 cut(s) 3311
SchI GAGTC 6 cut(s) 17, 674, 802, 2086, 2336, 2889
ScrFI CCNGG 2 cut(s) 1869, 3237
SduI GDGCHC 8 cut(s) 165, 353, 729, 775, 976, 2499, 3064, 3370
SfcI CTRYAG 4 cut(s) 2801, 3135, 3648, 3849
SinI GGWCC 4 cut(s) 1344, 1984, 3008, 3776
SmiI ATTTAAAT 1 cut(s) 3340
SmiMI CAYNNNNRTG 3 cut(s) 2921, 3737, 3750
SmlI CTYRAG 7 cut(s) 662, 728, 810, 1939, 2465, 2792, 2859
SmoI CTYRAG 7 cut(s) 662, 728, 810, 1939, 2465, 2792, 2859
SpeI ACTAGT 1 cut(s) 2197
SphI GCATGC 1 cut(s) 3188
SsiI CCGC 8 cut(s) 12, 49, 52, 281, 353, 956, 1755, 3006
SspI AATATT 4 cut(s) 1305, 2037, 3246, 3682
SstI GAGCTC 2 cut(s) 729, 2499
StyD4I CCNGG 2 cut(s) 1867, 3235
StyI CCWWGG 2 cut(s) 2232, 3075
SwaI ATTTAAAT 1 cut(s) 3340
TaiI ACGT 4 cut(s) 216, 326, 3200, 3382
TaqI TCGA 7 cut(s) 330, 362, 447, 1231, 1528, 2617, 4000
TatI WGTACW 2 cut(s) 3309, 3518
TauI GCSGC 1 cut(s) 54
TseFI GTSAC 2 cut(s) 1274, 3193
Tsp45I GTSAC 2 cut(s) 1274, 3193
TspGWI ACGGA 1 cut(s) 1997
Van91I CCANNNNNTGG 1 cut(s) 1354
Vha464I CTTAAG 1 cut(s) 1939
VpaK11BI GGWCC 4 cut(s) 1344, 1984, 3008, 3776
VspI ATTAAT 3 cut(s) 1992, 2457, 3735
XbaI TCTAGA 1 cut(s) 1843
XceI RCATGY 6 cut(s) 651, 1832, 2741, 2763, 3188, 3233
XcmI CCANNNNNNNNNTGG 2 cut(s) 2276, 3703
XmnI GAANNNNTTC 4 cut(s) 249, 570, 1290, 1789
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 1 cut(s) 3311
Zsp2I ATGCAT 1 cut(s) 2761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.