pycom15g31710

3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007261
Physical Location & Seq
Reverse (-)
30247272 .. 30252191
4920 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3282 bp
ATGAAAGGCCCGGTTGTTCAATCGGTTGTTGAAAGGGTTGAAGAAGGCGATAAGGAGAAGAAGAAGAGGCGCCGATCCAATCGCCGATCCAAGCAAAACAATTCCACTTCAGCAGCTAGTCGGATAAATGAAATACGAAGTGAAGTATCTGAGTGCCTGGCAAATGGAAGTATGGCTCATCATGTAAACACATCCTTGAAGCAACTTCAATTAGACATGCATTCTCCATATGAGCACGGAATGATCAAAGCATCTAATTTTGCCTTCCATTCATTGCCAACAATGCACATCAATGAACAAGTCAACCACGAGGATGTGCAGATTTTGGTAAATCAGCATTCTCTCCAATGTGATCCTTCCGAAAGAGTTATTGCACATTCTTGTCCTGAACCTGTGGCTTGTAGAGGATCACCTGGGATGTTCAAGGATTTCCCTCCTCATAACATTGAGAGTCATGCTCGGAGAAAATGTTTTACTTCACACTGGCCCATAGAGGCTGTTAATGATGCTCTGGAGGCCTATTGCAATATTGATGGTGTACCAACGGATGTTCTCGTTGGAGGACATGCTGAGCAGAATAGAGCTGTGGAAGGAGACATCGTGGCGATTAAGGTTGATCCCTTAGCGTTATGGACTAGGATGAAAGGATCAGCTGGGACTTGTACCAGCTCTGCACTGGTGGAGGATTTTAACTTACCACAAGAAGCTAATGAAATTTCTGGTCTTAACTGCAAAGGTAAAGATAAGGTAGAGGAAGTGAATTTGTATGATAATGATAGAAGAAGCCTGTTTCCTGAGAGGGGCTCACGTCCTGAGGAAAGTAATACCCTTGGTGAATCTGTTAATTCAGAGCAAATTGGACCAACAAGTTATGATCATGTCACTGGAAGGTACCCCTTGGCTTCAGATTCTTTACAAGCAGGTTCTCATGGTGAGCAGAATGAAGTTGCAGTTGCAGTAGAGTGGATGTGTGCCACGATCAGTTCATTCCCTTCAAAACGACCAACTGGCAGGGTTGTTGCTATCATTGAAAGGTCTCCTCGTCGAGACACTGTTGTTGGTTTTCTTCATGTTAAGAAGTGGATTGCTTACAGGGAGGTTTGCAGAAACGACACGAGAAAGAAAAAAAATGCATCATTTTCTAACAACGAGTACATTCAGATGACCCCAACTGACCCCAGATTTCCAAAAATGGTGGTCCTTGTGAGAACTCTGCCTGATTCGATCAAGAAAAGATTGGAGAATGGCGATGAGTCAATCGAAATGGAGTTGTTTGCTGCCCGAATAGATGAATGGGATGAACAAAGTTCTGCTCCTCAAGCTGTCATCTTGAATGCTTTTGGGCGGGCAGGTGAAGTACAGCCACAAGTTGAAGCAATTTTATTCCAAAATTCAATTAATTCGTCTGATTTTTGTCATGAATCACTTTCCTGTCTTCCTCCTCTCCCATGGGAGGTGCCTCAGGAGGAAATCAAAAGTAGAAAGGATCTTCGAAACCTGTTCATATTTACCATCGACCCTTCCACTGCTACTGATCTGGATGATGCTCTCTCAATTGAGAAATTACCCAATGGAATTTTCAGAGTTGGTATCCACATTGCTGATGTGTCGTATTTTCTTTTGCCCGACACACCCTTGGACGATGAAGCTCAATCTAGATCAACAAGTGTGTATATGTCACAGCGGAAATTACCAATGTTGCCTCCTTTGCTCTCAGAGGATATTGGTTCGCTTAACCCAGGAGTAGAGAGACTAGCATTTTCAATTTTCTTTGACATCAACCATGTTGGGGATGTCGTTGACCGATGGATTGGCCGCACTGTAATAAGATCTTGTTGCAAGCTTGCTTATGAACATGCTCAGGAGATTATCAATGGGAAACTTAATTTGGAAAGTTCTAACATTTTAGGACATGGTTGTCCTCAGTTGCATGGCCATTTTGAATGGTCTGATGTAATTAGATCTGTCAAAGACCTTCATGAAATTTCCAGAGTTTTGAAGGAGAGGAGGTTTAGTGATGGAGCTCTTCAGCTTGAAAGCTCCAAAGTTGTTATTCTGTTTGACGAAGATGGAGTTCCATATGATAGCATGTATTCTGAATGGAAGGACTCCAATTTTCTTGTCGAGGAGTTTATGCTTTTAGCAAATAGAGCAGCGGCTGAAGTTATATCTAGAGCTTTTCCTGAGAATGCATTACTGCGGAGGCACCCAGAACCTACTATGCGCAAACTCAGGGAATTTGAAGCATTTTGTTCTAAGCATGGTTTGGAGTTGGATACGTCTTCTTCTGGGCAGTTCCAGCAATCATTGCAGAGAATCAGGGAAGAGCTCAAGGATGACTCTGTTCTATTCAATATTCTTATGAATTATGCTACAAAACCAATGCAGTTGGCTTCTTATTTTTGTAGTGGCCAATTAAAAGATAGAGAAAATGATTGGGGTCACTATGGACTTGCTGTTCCTTTGTACACTCATTTTACGTCACCACTGCGTCGGTATCCAGATATTGTAGTGCATCGCACGTTGGCTGCAGTTATAGAGGCAGAGGAGTTATTTTTGAAACGTCGGAGAATATTGAATAATTTTAGCAGGGGAGATGAAGTTAAAATGAGATGTTTCACTGGTATTTATTTTGATAAGGATGTTGCTGAATCCTGTGAAATCAAGGAAGCATTATCGGCAGCAGCAATAAAGCAAAGAGTTCCCTGCTCTGAAATGCTTGCTGATGTTTCCGCTTATTGCAATGAAAGAAAGCTGGCTAGTAGACATGTCAAAAATGCCTGTGATAAGCTATACATGTGGGCCCTGCTGAAGAAAAAGGAGATTGTCCTATCAGAAGCAAGAGTATTGGGTGCAGGACCTAGATTCATGTCCATTTATATTCACAAGCTTGCTGTTGAGCGTCGAATATATTATGATGAAGTTGAAGGCTTGATGGTAGAATGGCTTGATGCTACGTCCACAGTGGTGCTGAATTTATGTTCTAACAGATGCTCACTCAGGAGAGGTTGCCCTGGTAAATGGAGGGCACTTGAAGATGTTGCCTTGGTTGTAAGTCCTTATGGCCTCAAAGCTGAACTAGGCGGAACTGGAAATAGTTCTAATGAGGGTGCTGGGACCCAAGATGTTGGCGGAGCCACCCATAGTGAAATCGATCCTGTGGTTTTCCCCCTTACACTGCGTGTTCTTTCAACCATTCCTGTAGTGCTTCATGCAGTTGGAGGGGATGATGGACCTGTCGATATTGGGGCAAGGCTGTACATGAGCTCATATCTCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000175 GO:0000178 GO:0000278 GO:0000280 GO:0000287 GO:0000291 GO:0000819 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005844 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0008285 GO:0008408 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010586 GO:0010587 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019222 GO:0019439 GO:0019827 GO:0022402 GO:0022613 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035770 GO:0036464 GO:0042127 GO:0042254 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0046872 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051276 GO:0051304 GO:0051306 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0097159 GO:0098727 GO:0098813 GO:0140014 GO:0140098 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1902494 GO:1903047 GO:1905354 GO:1990074 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNB

Protein Analysis

1094

Amino Acids

122.67

Weight (kDa)

5.86

Isoelectric Point (pI)

56.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Dis3 PF17849 385 - 464 4.4e-16 Dis3-like cold-shock domain 2 (CSD2)
RNB PF00773 494 - 847 3.2e-101 RNB domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1340
AasI GACNNNNNNGTC 1 cut(s) 1917
Acc16I TGCGCA 1 cut(s) 2225
Acc36I ACCTGC 2 cut(s) 911, 1340
Acc65I GGTACC 1 cut(s) 891
AccB1I GGYRCC 4 cut(s) 69, 891, 1456, 2205
AccI GTMKAC 1 cut(s) 2764
AciI CCGC 8 cut(s) 1345, 1684, 1816, 2156, 2200, 2733, 3084, 3132
AclWI GGATC 8 cut(s) 69, 81, 347, 415, 611, 655, 1494, 3149
AcoI YGGCCR 3 cut(s) 1813, 1933, 2410
AcsI RAATTY 7 cut(s) 714, 760, 1390, 1575, 1983, 2237, 2974
AcuI CTGAAG 5 cut(s) 93, 888, 2012, 2181, 2831
AcyI GRCGYC 1 cut(s) 70
AdeI CACNNNGTG 1 cut(s) 3182
AfaI GTAC 7 cut(s) 540, 664, 893, 1154, 1359, 2468, 3260
AfiI CCNNNNNNNGG 3 cut(s) 800, 1453, 1789
AflIII ACRYGT 2 cut(s) 2767, 2796
AjiI CACGTC 1 cut(s) 809
AjnI CCWGG 4 cut(s) 156, 412, 1738, 3013
AjuI GAANNNNNNNTTGG 4 cut(s) 1562, 1594, 1871, 1903
AleI CACNNNNGTG 1 cut(s) 2966
AloI GAACNNNNNNTCC 2 cut(s) 2442, 2474
Alw21I GWGCWC 4 cut(s) 237, 2026, 2331, 3269
Alw26I GTCTC 4 cut(s) 588, 1041, 1041, 1744
AlwI GGATC 8 cut(s) 69, 81, 347, 415, 611, 655, 1494, 3149
AlwNI CAGNNNCTG 1 cut(s) 2159
AoxI GGCC 8 cut(s) 7, 485, 516, 1813, 1933, 2410, 2802, 3064
ApaI GGGCCC 1 cut(s) 2806
ApeKI GCWGC 6 cut(s) 113, 1277, 2153, 2528, 2681, 2684
ApoI RAATTY 7 cut(s) 714, 760, 1390, 1575, 1983, 2237, 2974
AseI ATTAAT 1 cut(s) 1398
Asp700I GAANNNNTTC 1 cut(s) 3097
Asp718I GGTACC 1 cut(s) 891
AspLEI GCGC 2 cut(s) 72, 2226
AspS9I GGNCC 9 cut(s) 8, 486, 860, 1198, 2802, 2803, 2858, 3117, 3233
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 5 cut(s) 402, 845, 944, 1364, 2475
AsuII TTCGAA 1 cut(s) 1492
AvaII GGWCC 5 cut(s) 860, 1198, 2858, 3117, 3233
AxyI CCTNAGG 2 cut(s) 813, 1461
BaeGI GKGCMC 2 cut(s) 2806, 3031
BalI TGGCCA 2 cut(s) 1935, 2412
BanI GGYRCC 4 cut(s) 69, 891, 1456, 2205
BanII GRGCYC 5 cut(s) 806, 2026, 2331, 2806, 3269
BauI CACGAG 2 cut(s) 308, 1114
BbsI GAAGAC 2 cut(s) 1427, 2274
Bbv12I GWGCWC 4 cut(s) 237, 2026, 2331, 3269
BbvI GCAGC 6 cut(s) 125, 1264, 2165, 2515, 2693, 2696
BccI CCATC 7 cut(s) 527, 1520, 1800, 2012, 2063, 2929, 3224
BciT130I CCWGG 4 cut(s) 158, 414, 1740, 3015
BciVI GTATCC 3 cut(s) 1601, 2269, 2508
BclI TGATCA 2 cut(s) 243, 874
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 4 cut(s) 588, 1041, 1041, 1744
BfaI CTAG 9 cut(s) 117, 636, 1656, 1754, 2172, 2760, 2862, 3080, 3280
BfmI CTRYAG 2 cut(s) 2529, 3201
BfoI RGCGCY 1 cut(s) 73
BfuAI ACCTGC 2 cut(s) 911, 1340
BfuI GTATCC 3 cut(s) 1601, 2269, 2508
BglII AGATCT 2 cut(s) 1829, 1961
BisI GCNGC 8 cut(s) 114, 1278, 1816, 2154, 2157, 2529, 2682, 2685
BlpI GCTNAGC 1 cut(s) 570
BlsI GCNGC 8 cut(s) 115, 1279, 1817, 2155, 2158, 2530, 2683, 2686
Bme1390I CCNGG 5 cut(s) 11, 158, 414, 1740, 3015
Bme18I GGWCC 5 cut(s) 860, 1198, 2858, 3117, 3233
BmgBI CACGTC 1 cut(s) 809
BmgT120I GGNCC 9 cut(s) 8, 486, 860, 1198, 2802, 2803, 2858, 3117, 3233
BmiI GGNNCC 8 cut(s) 71, 893, 1458, 2207, 2804, 3118, 3119, 3136
BmrFI CCNGG 5 cut(s) 11, 158, 414, 1740, 3015
BmsI GCATC 7 cut(s) 260, 496, 1142, 1534, 2524, 2941, 2981
BpiI GAAGAC 2 cut(s) 1427, 2274
BplI GAGNNNNNCTC 4 cut(s) 442, 474, 788, 820
BpmI CTGGAG 1 cut(s) 533
Bpu10I CCTNAGC 2 cut(s) 622, 1860
Bpu1102I GCTNAGC 1 cut(s) 570
Bpu14I TTCGAA 1 cut(s) 1492
BpuEI CTTGAG 2 cut(s) 1302, 2315
BpuMI CCSGG 1 cut(s) 11
Bsa29I ATCGAT 1 cut(s) 3153
BsaHI GRCGYC 1 cut(s) 70
BsaI GGTCTC 1 cut(s) 1041
BsaJI CCNNGG 8 cut(s) 413, 829, 897, 1448, 1635, 1738, 3013, 3045
Bsc4I CCNNNNNNNGG 3 cut(s) 800, 1453, 1789
Bse1I ACTGG 6 cut(s) 488, 681, 889, 1012, 2626, 3094
Bse21I CCTNAGG 2 cut(s) 813, 1461
Bse3DI GCAATG 4 cut(s) 272, 1596, 2306, 2749
BseBI CCWGG 4 cut(s) 158, 414, 1740, 3015
BseCI ATCGAT 1 cut(s) 3153
BseDI CCNNGG 8 cut(s) 413, 829, 897, 1448, 1635, 1738, 3013, 3045
BseLI CCNNNNNNNGG 3 cut(s) 800, 1453, 1789
BseMI GCAATG 4 cut(s) 272, 1596, 2306, 2749
BseNI ACTGG 6 cut(s) 488, 681, 889, 1012, 2626, 3094
BseRI GAGGAG 7 cut(s) 426, 1029, 1305, 1431, 2020, 2141, 2561
BseSI GKGCMC 2 cut(s) 2806, 3031
BseXI GCAGC 6 cut(s) 125, 1264, 2165, 2515, 2693, 2696
BseYI CCCAGC 2 cut(s) 653, 3113
BsgI GTGCAG 3 cut(s) 338, 657, 2874
BshFI GGCC 8 cut(s) 9, 487, 518, 1815, 1935, 2412, 2804, 3066
BshNI GGYRCC 4 cut(s) 69, 891, 1456, 2205
BshVI ATCGAT 1 cut(s) 3153
BsiHKAI GWGCWC 4 cut(s) 237, 2026, 2331, 3269
BsiSI CCGG 1 cut(s) 11
BslFI GGGAC 2 cut(s) 670, 3130
BslI CCNNNNNNNGG 3 cut(s) 800, 1453, 1789
BsmAI GTCTC 4 cut(s) 588, 1041, 1041, 1744
BsmFI GGGAC 2 cut(s) 670, 3130
BsmI GAATGC 4 cut(s) 220, 337, 1339, 2194
BsnI GGCC 8 cut(s) 9, 487, 518, 1815, 1935, 2412, 2804, 3066
Bso31I GGTCTC 1 cut(s) 1041
Bsp119I TTCGAA 1 cut(s) 1492
Bsp120I GGGCCC 1 cut(s) 2802
Bsp1286I GDGCHC 7 cut(s) 237, 806, 2026, 2331, 2806, 3031, 3269
Bsp1407I TGTACA 2 cut(s) 2466, 3258
Bsp1720I GCTNAGC 1 cut(s) 570
Bsp19I CCATGG 1 cut(s) 1448
BspACI CCGC 8 cut(s) 1345, 1684, 1816, 2156, 2200, 2733, 3084, 3132
BspANI GGCC 8 cut(s) 9, 487, 518, 1815, 1935, 2412, 2804, 3066
BspDI ATCGAT 1 cut(s) 3153
BspHI TCATGA 2 cut(s) 1417, 1978
BspLI GGNNCC 8 cut(s) 71, 893, 1458, 2207, 2804, 3118, 3119, 3136
BspMAI CTGCAG 1 cut(s) 2533
BspMI ACCTGC 2 cut(s) 911, 1340
BspPI GGATC 8 cut(s) 69, 81, 347, 415, 611, 655, 1494, 3149
BspQI GCTCTTC 2 cut(s) 2031, 2319
BspT104I TTCGAA 1 cut(s) 1492
BspT107I GGYRCC 4 cut(s) 69, 891, 1456, 2205
BspTNI GGTCTC 1 cut(s) 1041
BsrDI GCAATG 4 cut(s) 272, 1596, 2306, 2749
BsrGI TGTACA 2 cut(s) 2466, 3258
BsrI ACTGG 6 cut(s) 488, 681, 889, 1012, 2626, 3094
BssECI CCNNGG 8 cut(s) 413, 829, 897, 1448, 1635, 1738, 3013, 3045
BssNI GRCGYC 1 cut(s) 70
BssSI CACGAG 2 cut(s) 308, 1114
BssT1I CCWWGG 5 cut(s) 829, 897, 1448, 1635, 3045
Bst2BI CACGAG 2 cut(s) 308, 1114
Bst2UI CCWGG 4 cut(s) 158, 414, 1740, 3015
Bst4CI ACNGT 3 cut(s) 1054, 1822, 2965
Bst6I CTCTTC 3 cut(s) 59, 2031, 2319
BstACI GRCGYC 1 cut(s) 70
BstAPI GCANNNNNTGC 1 cut(s) 2308
BstAUI TGTACA 2 cut(s) 2466, 3258
BstBI TTCGAA 1 cut(s) 1492
BstC8I GCNNGC 6 cut(s) 1347, 1841, 1845, 2721, 2757, 2892
BstDSI CCRYGG 1 cut(s) 1448
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 2 cut(s) 72, 2226
BstMAI GTCTC 4 cut(s) 588, 1041, 1041, 1744
BstMWI GCNNNNNNNGC 8 cut(s) 283, 515, 1319, 1708, 2150, 2299, 2308, 2678
BstNI CCWGG 4 cut(s) 158, 414, 1740, 3015
BstNSI RCATGY 6 cut(s) 220, 569, 1859, 2092, 2771, 2800
BstSCI CCNGG 5 cut(s) 9, 156, 412, 1738, 3013
BstSFI CTRYAG 2 cut(s) 2529, 3201
BstSLI GKGCMC 2 cut(s) 2806, 3031
BstV1I GCAGC 6 cut(s) 125, 1264, 2165, 2515, 2693, 2696
BstV2I GAAGAC 2 cut(s) 1427, 2274
BstX2I RGATCY 3 cut(s) 1486, 1829, 1961
BstXI CCANNNNNNTGG 1 cut(s) 3128
BstYI RGATCY 3 cut(s) 1486, 1829, 1961
Bsu15I ATCGAT 1 cut(s) 3153
Bsu36I CCTNAGG 2 cut(s) 813, 1461
BsuI GTATCC 3 cut(s) 1601, 2269, 2508
BsuRI GGCC 8 cut(s) 9, 487, 518, 1815, 1935, 2412, 2804, 3066
BsuTUI ATCGAT 1 cut(s) 3153
BtgI CCRYGG 1 cut(s) 1448
BtgZI GCGATG 2 cut(s) 1263, 2501
BtrI CACGTC 1 cut(s) 809
BtsI GCAGTG 3 cut(s) 1524, 2486, 3176
BveI ACCTGC 2 cut(s) 911, 1340
Cac8I GCNNGC 6 cut(s) 1347, 1841, 1845, 2721, 2757, 2892
CaiI CAGNNNCTG 1 cut(s) 2159
CciI TCATGA 2 cut(s) 1417, 1978
CfoI GCGC 2 cut(s) 72, 2226
Cfr13I GGNCC 9 cut(s) 8, 486, 860, 1198, 2802, 2803, 2858, 3117, 3233
ClaI ATCGAT 1 cut(s) 3153
CseI GACGC 2 cut(s) 2480, 2891
Csp6I GTAC 7 cut(s) 539, 663, 892, 1153, 1358, 2467, 3259
CspCI CAANNNNNGTGG 2 cut(s) 1176, 1211
CviQI GTAC 7 cut(s) 539, 663, 892, 1153, 1358, 2467, 3259
DinI GGCGCC 1 cut(s) 71
DraIII CACNNNGTG 1 cut(s) 3182
DrdI GACNNNNNNGTC 1 cut(s) 1917
DseDI GACNNNNNNGTC 1 cut(s) 1917
EaeI YGGCCR 3 cut(s) 1813, 1933, 2410
Eam1104I CTCTTC 3 cut(s) 59, 2031, 2319
EarI CTCTTC 3 cut(s) 59, 2031, 2319
EciI GGCGGA 2 cut(s) 3099, 3147
Ecl136II GAGCTC 3 cut(s) 2024, 2329, 3267
Eco130I CCWWGG 5 cut(s) 829, 897, 1448, 1635, 3045
Eco147I AGGCCT 1 cut(s) 518
Eco24I GRGCYC 5 cut(s) 806, 2026, 2331, 2806, 3269
Eco31I GGTCTC 1 cut(s) 1041
Eco47I GGWCC 5 cut(s) 860, 1198, 2858, 3117, 3233
Eco53kI GAGCTC 3 cut(s) 2024, 2329, 3267
Eco57I CTGAAG 5 cut(s) 93, 888, 2012, 2181, 2831
Eco81I CCTNAGG 2 cut(s) 813, 1461
EcoICRI GAGCTC 3 cut(s) 2024, 2329, 3267
EcoO109I RGGNCCY 3 cut(s) 2803, 2858, 3117
EcoRII CCWGG 4 cut(s) 156, 412, 1738, 3013
EcoT14I CCWWGG 5 cut(s) 829, 897, 1448, 1635, 3045
EcoT22I ATGCAT 3 cut(s) 222, 1135, 2194
EcoT38I GRGCYC 5 cut(s) 806, 2026, 2331, 2806, 3269
EgeI GGCGCC 1 cut(s) 71
EheI GGCGCC 1 cut(s) 71
ErhI CCWWGG 5 cut(s) 829, 897, 1448, 1635, 3045
FaqI GGGAC 2 cut(s) 670, 3130
FauI CCCGC 1 cut(s) 1338
FauNDI CATATG 2 cut(s) 229, 2080
FbaI TGATCA 2 cut(s) 243, 874
FblI GTMKAC 1 cut(s) 2764
Fnu4HI GCNGC 8 cut(s) 114, 1278, 1816, 2154, 2157, 2529, 2682, 2685
FriOI GRGCYC 5 cut(s) 806, 2026, 2331, 2806, 3269
Fsp4HI GCNGC 8 cut(s) 114, 1278, 1816, 2154, 2157, 2529, 2682, 2685
FspBI CTAG 9 cut(s) 117, 636, 1656, 1754, 2172, 2760, 2862, 3080, 3280
FspI TGCGCA 1 cut(s) 2225
GlaI GCGC 2 cut(s) 71, 2225
GluI GCNGC 8 cut(s) 114, 1278, 1816, 2154, 2157, 2529, 2682, 2685
GsaI CCCAGC 2 cut(s) 657, 3117
GsuI CTGGAG 1 cut(s) 533
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 8 cut(s) 9, 487, 518, 1815, 1935, 2412, 2804, 3066
HapII CCGG 1 cut(s) 11
HgaI GACGC 2 cut(s) 2480, 2891
HhaI GCGC 2 cut(s) 72, 2226
Hin1I GRCGYC 1 cut(s) 70
Hin6I GCGC 2 cut(s) 70, 2224
HinP1I GCGC 2 cut(s) 70, 2224
HincII GTYRAC 2 cut(s) 304, 1801
HindII GTYRAC 2 cut(s) 304, 1801
HindIII AAGCTT 2 cut(s) 1841, 2888
HpaII CCGG 1 cut(s) 11
HphI GGTGA 5 cut(s) 402, 845, 944, 1364, 2475
Hpy166II GTNNAC 7 cut(s) 187, 304, 539, 1801, 2469, 2765, 2961
Hpy8I GTNNAC 7 cut(s) 187, 304, 539, 1801, 2469, 2765, 2961
Hpy99I CGWCG 4 cut(s) 1047, 2496, 2568, 2907
HpyCH4III ACNGT 3 cut(s) 1054, 1822, 2965
HpyCH4IV ACGT 6 cut(s) 808, 2279, 2480, 2522, 2563, 2957
HpyF10VI GCNNNNNNNGC 8 cut(s) 283, 515, 1319, 1708, 2150, 2299, 2308, 2678
HpySE526I ACGT 6 cut(s) 808, 2279, 2480, 2522, 2563, 2957
Hsp92I GRCGYC 1 cut(s) 70
HspAI GCGC 2 cut(s) 70, 2224
KasI GGCGCC 1 cut(s) 69
KflI GGGWCCC 1 cut(s) 3117
KpnI GGTACC 1 cut(s) 895
Ksp22I TGATCA 2 cut(s) 243, 874
LguI GCTCTTC 2 cut(s) 2031, 2319
LmnI GCTCC 4 cut(s) 1318, 2021, 2045, 3134
Lsp1109I GCAGC 6 cut(s) 125, 1264, 2165, 2515, 2693, 2696
LweI GCATC 7 cut(s) 260, 496, 1142, 1534, 2524, 2941, 2981
MaeI CTAG 9 cut(s) 117, 636, 1656, 1754, 2172, 2760, 2862, 3080, 3280
MaeII ACGT 6 cut(s) 808, 2279, 2480, 2522, 2563, 2957
MaeIII GTNAC 4 cut(s) 880, 1677, 2441, 2481
MfeI CAATTG 1 cut(s) 1554
MflI RGATCY 3 cut(s) 1486, 1829, 1961
MhlI GDGCHC 7 cut(s) 237, 806, 2026, 2331, 2806, 3031, 3269
MlsI TGGCCA 2 cut(s) 1935, 2412
MluNI TGGCCA 2 cut(s) 1935, 2412
Mly113I GGCGCC 1 cut(s) 70
MlyI GAGTC 4 cut(s) 460, 1262, 2102, 2333
MmeI TCCRAC 5 cut(s) 101, 538, 2253, 2546, 3199
Mox20I TGGCCA 2 cut(s) 1935, 2412
Mph1103I ATGCAT 3 cut(s) 222, 1135, 2194
MroXI GAANNNNTTC 1 cut(s) 3097
MscI TGGCCA 2 cut(s) 1935, 2412
MslI CAYNNNNRTG 4 cut(s) 291, 312, 1160, 2966
Msp20I TGGCCA 2 cut(s) 1935, 2412
MspA1I CMGCKG 3 cut(s) 653, 1684, 2156
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 5 cut(s) 11, 158, 414, 1740, 3015
MunI CAATTG 1 cut(s) 1554
Mva1269I GAATGC 4 cut(s) 220, 337, 1339, 2194
MvaI CCWGG 4 cut(s) 158, 414, 1740, 3015
MwoI GCNNNNNNNGC 8 cut(s) 283, 515, 1319, 1708, 2150, 2299, 2308, 2678
NarI GGCGCC 1 cut(s) 70
NciI CCSGG 1 cut(s) 11
NcoI CCATGG 1 cut(s) 1448
NdeI CATATG 2 cut(s) 229, 2080
NlaIV GGNNCC 8 cut(s) 71, 893, 1458, 2207, 2804, 3118, 3119, 3136
NmuCI GTSAC 4 cut(s) 880, 1677, 2441, 2481
NsbI TGCGCA 1 cut(s) 2225
NsiI ATGCAT 3 cut(s) 222, 1135, 2194
NspI RCATGY 6 cut(s) 220, 569, 1859, 2092, 2771, 2800
NspV TTCGAA 1 cut(s) 1492
OliI CACNNNNGTG 1 cut(s) 2966
PagI TCATGA 2 cut(s) 1417, 1978
PaqCI CACCTGC 1 cut(s) 1340
PceI AGGCCT 1 cut(s) 518
PciI ACATGT 2 cut(s) 2767, 2796
PciSI GCTCTTC 2 cut(s) 2031, 2319
PctI GAATGC 4 cut(s) 220, 337, 1339, 2194
PdmI GAANNNNTTC 1 cut(s) 3097
PfeI GAWTC 7 cut(s) 836, 908, 1220, 1421, 2316, 2651, 2865
PkrI GCNGC 8 cut(s) 115, 1279, 1817, 2155, 2158, 2530, 2683, 2686
PleI GAGTC 4 cut(s) 459, 1261, 2102, 2333
PluTI GGCGCC 1 cut(s) 73
PpsI GAGTC 4 cut(s) 459, 1261, 2102, 2333
PpuMI RGGWCCY 2 cut(s) 2858, 3117
PscI ACATGT 2 cut(s) 2767, 2796
PshBI ATTAAT 1 cut(s) 1398
Psp124BI GAGCTC 3 cut(s) 2026, 2331, 3269
Psp5II RGGWCCY 2 cut(s) 2858, 3117
Psp6I CCWGG 4 cut(s) 156, 412, 1738, 3013
PspFI CCCAGC 2 cut(s) 653, 3113
PspGI CCWGG 4 cut(s) 156, 412, 1738, 3013
PspN4I GGNNCC 8 cut(s) 71, 893, 1458, 2207, 2804, 3118, 3119, 3136
PspOMI GGGCCC 1 cut(s) 2802
PspPI GGNCC 9 cut(s) 8, 486, 860, 1198, 2802, 2803, 2858, 3117, 3233
PspPPI RGGWCCY 2 cut(s) 2858, 3117
PstI CTGCAG 1 cut(s) 2533
PstNI CAGNNNCTG 1 cut(s) 2159
PsuI RGATCY 3 cut(s) 1486, 1829, 1961
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 7 cut(s) 540, 664, 893, 1154, 1359, 2468, 3260
RsaNI GTAC 7 cut(s) 539, 663, 892, 1153, 1358, 2467, 3259
RseI CAYNNNNRTG 4 cut(s) 291, 312, 1160, 2966
SacI GAGCTC 3 cut(s) 2026, 2331, 3269
SapI GCTCTTC 2 cut(s) 2031, 2319
SatI GCNGC 8 cut(s) 114, 1278, 1816, 2154, 2157, 2529, 2682, 2685
Sau96I GGNCC 9 cut(s) 8, 486, 860, 1198, 2802, 2803, 2858, 3117, 3233
SchI GAGTC 4 cut(s) 460, 1262, 2102, 2333
ScrFI CCNGG 5 cut(s) 11, 158, 414, 1740, 3015
SduI GDGCHC 7 cut(s) 237, 806, 2026, 2331, 2806, 3031, 3269
SfaNI GCATC 7 cut(s) 260, 496, 1142, 1534, 2524, 2941, 2981
SfcI CTRYAG 2 cut(s) 2529, 3201
SfoI GGCGCC 1 cut(s) 71
SfuI TTCGAA 1 cut(s) 1492
SinI GGWCC 5 cut(s) 860, 1198, 2858, 3117, 3233
SmiMI CAYNNNNRTG 4 cut(s) 291, 312, 1160, 2966
SmlI CTYRAG 2 cut(s) 1317, 2330
SmoI CTYRAG 2 cut(s) 1317, 2330
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 8 cut(s) 1345, 1684, 1816, 2156, 2200, 2733, 3084, 3132
SspDI GGCGCC 1 cut(s) 69
SspI AATATT 3 cut(s) 529, 2356, 2574
SspMI CTAG 9 cut(s) 117, 636, 1656, 1754, 2172, 2760, 2862, 3080, 3280
SstI GAGCTC 3 cut(s) 2026, 2331, 3269
StuI AGGCCT 1 cut(s) 518
StyD4I CCNGG 5 cut(s) 9, 156, 412, 1738, 3013
StyI CCWWGG 5 cut(s) 829, 897, 1448, 1635, 3045
TaaI ACNGT 3 cut(s) 1054, 1822, 2965
TaiI ACGT 6 cut(s) 811, 2282, 2483, 2525, 2566, 2960
TaqI TCGA 9 cut(s) 1045, 1223, 1260, 1492, 1515, 2124, 2905, 3153, 3240
TaqII GACCGA 1 cut(s) 1818
TatI WGTACW 4 cut(s) 1152, 1357, 2466, 3258
TauI GCSGC 2 cut(s) 1818, 2159
TfiI GAWTC 7 cut(s) 836, 908, 1220, 1421, 2316, 2651, 2865
TseFI GTSAC 4 cut(s) 880, 1677, 2441, 2481
TseI GCWGC 6 cut(s) 113, 1277, 2153, 2528, 2681, 2684
Tsp45I GTSAC 4 cut(s) 880, 1677, 2441, 2481
TspGWI ACGGA 2 cut(s) 252, 560
VpaK11BI GGWCC 5 cut(s) 860, 1198, 2858, 3117, 3233
VspI ATTAAT 1 cut(s) 1398
XapI RAATTY 7 cut(s) 714, 760, 1390, 1575, 1983, 2237, 2974
XbaI TCTAGA 2 cut(s) 1655, 2171
XceI RCATGY 6 cut(s) 220, 569, 1859, 2092, 2771, 2800
XcmI CCANNNNNNNNNTGG 1 cut(s) 673
XmiI GTMKAC 1 cut(s) 2764
XmnI GAANNNNTTC 1 cut(s) 3097
XspI CTAG 9 cut(s) 117, 636, 1656, 1754, 2172, 2760, 2862, 3080, 3280
Zsp2I ATGCAT 3 cut(s) 222, 1135, 2194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.