pycom15g31810

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008279
Physical Location & Seq
Forward (+)
30587051 .. 30590593
3543 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1728 bp
ATGGGAATGAAAGCAAAGGCCCACCACCACCATTACAATGCTAGTGGTGGTAGTGGTGGTGGAGGCAACGGCAGTGAGAGCAGCAGCAGCACCTCACCTAGCCCACCTCCGTCGCCGCGCCGTCACTCTACAGTCCCTCACTGCCGCCGGAAGCTCCGGGCAAAACCCATCTCCATCGTCTCCGGTTTTCTTCTCCTCCGCCGATTCCTCCGCTTACTGGTGGTTCTTCCCCTGCTATACGTCTCTGGCCTGCTCATGTGTGTGGGCCCGTTCTCTGGTCTACTGGGTCATACCCCTCTTCCCGGTTCCGTTTACCGAAGTCACGAGATGTTTCAGCTGCTCTGGCCTCACATTCAGGCCGATAATTCCACTCCGATTGAGTTGTCCTCTGTATGGAGATTCAGAAGGAAGTTGAAAGAGCAGAAGCCTTGCCCACCTTCAGCTTCTGGACTACAATTAGATTCCCCAGGCCTTACTGGTTACTTGATTGTGGAGGTTAATGGTGGCCTTAACCAACAACGCTCTGCGATTTGCAATGCTGTGGCCCTTGCTGGTCTTTTGAATGCAGTTTTGGTTATTCCTCACTTTGAATTCCATAACGTTTGGAGGGATCCGAGCACTTTTACTGATATATACGATGAAGATCATTTCATAGCCACCCTTGAAGGCCATGTTAAAGTGGTTAAAGAACTTCCGGAGATTCTGATGGATAAATATGATCACAACATCTCTAACATACCAACCCTTCGTGTCGAAGCTTGGGCTCCTGTCCGTTATTACACAGGAGAAGTTTATCCTGTTCTGCAGAAGCAGGGGGTTATCCGCATAGCTCCATTTGCTAATAGATTAGCAATGAATGTCCCACCTCCTATTCAGCATTTGAGATGCCTTGCTAATTATGAAGCATTGAAATTCTCTTCTGCCATATCAAATTTGGCAAATAAACTAGTTGAACGAATGACTGAAATGAGTTCAAGGACCGGTGGAAAGTACGTTGCAGTCCACCTCCGTTTTGAGGAGGACATGGTGGCATTCTCGTGCTGTTTGTATGATGGAGGAGAGGCTGAAAATGTCGCAATGAATTCAATTCGACAAAAAGGATGGGGAGAGAAGTTCAAGAGAAAAGGCCGCGTAACTCTACCTGGTCTCAACAGGATTGATGGAAAATGCCCACTTACCCCCTTGGAGGTTGGAATGATACTACGGGGTATGGGATTTGATAACAGCACTTCAATTTATTTAGCCTCTGGGAAAATCTACTGGGCGGAGAAACATTTGGCTCCTCTGCTCAAGTTGTTTCCCTATCTGCATACCAAGGAGTCTCTTGCAACCCCAGATGAGCTTGCTCCTTTTGAGGGTTATTCTTCAAGATTGGCAGCTTTGGATTATACAGTATGCTTGTTTAGTGAGATTTTTGTGACAACACAAGGTGGGAACTTCCCACATTTTCTAATGGGTCATCGGAGATTCCTCTATGGTGGGCATTCCAAGACTATTATACCTGATAAGCGCAAGCTTGTTGTTCTACTGCAGAACATGAGTATGAGTTGGAAAGATTTCAAACTTGAGATGGAAGCAATGCTTACAGAAAGCGACCGCAAGGGAAAGATAATACCGAGAGTGAAAAAATTCACACGAAAATCTTCTGTATACACATACCCTTTGCCAGAGTGTAGTTGTCTCCAAAAGCACAACAACACTCAGATTGTACCGTCTACACATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

576

Amino Acids

64.32

Weight (kDa)

9.45

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 161 - 490 4.1e-97 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 280, 1650, 1715
AccII CGCG 2 cut(s) 118, 1131
AccIII TCCGGA 1 cut(s) 694
AciI CCGC 8 cut(s) 116, 145, 199, 211, 823, 1129, 1265, 1597
AclI AACGTT 1 cut(s) 600
AclWI GGATC 2 cut(s) 605, 618
AcsI RAATTY 5 cut(s) 590, 911, 931, 1081, 1628
AcuI CTGAAG 1 cut(s) 423
AdeI CACNNNGTG 1 cut(s) 1430
AfaI GTAC 2 cut(s) 992, 1710
AfiI CCNNNNNNNGG 7 cut(s) 217, 275, 302, 393, 1015, 1186, 1355
AgeI ACCGGT 1 cut(s) 980
AhlI ACTAGT 1 cut(s) 946
AjnI CCWGG 2 cut(s) 466, 1141
AjuI GAANNNNNNNTTGG 2 cut(s) 554, 586
AluBI AGCT 8 cut(s) 154, 337, 443, 758, 830, 1342, 1379, 1516
AluI AGCT 8 cut(s) 154, 337, 443, 758, 830, 1342, 1379, 1516
Alw21I GWGCWC 1 cut(s) 620
Alw26I GTCTC 5 cut(s) 184, 247, 1151, 1326, 1685
AlwI GGATC 2 cut(s) 605, 618
AlwNI CAGNNNCTG 1 cut(s) 446
Aor13HI TCCGGA 1 cut(s) 694
ApaI GGGCCC 1 cut(s) 269
ApeKI GCWGC 5 cut(s) 81, 84, 87, 337, 1376
ApoI RAATTY 5 cut(s) 590, 911, 931, 1081, 1628
AsiGI ACCGGT 1 cut(s) 980
Asp700I GAANNNNTTC 3 cut(s) 1556, 1628, 1642
AspLEI GCGC 2 cut(s) 120, 1512
AspS9I GGNCC 5 cut(s) 19, 265, 266, 544, 978
AsuC2I CCSGG 2 cut(s) 158, 303
AsuHPI GGTGA 1 cut(s) 87
AvaII GGWCC 1 cut(s) 978
BaeGI GKGCMC 1 cut(s) 269
BamHI GGATCC 1 cut(s) 610
BanII GRGCYC 2 cut(s) 269, 766
BauI CACGAG 2 cut(s) 323, 1036
Bbv12I GWGCWC 1 cut(s) 620
BbvI GCAGC 5 cut(s) 93, 96, 99, 324, 1388
BccI CCATC 7 cut(s) 176, 182, 700, 1046, 1095, 1154, 1564
BceAI ACGGC 2 cut(s) 85, 105
BciT130I CCWGG 2 cut(s) 468, 1143
BclI TGATCA 1 cut(s) 718
BcnI CCSGG 2 cut(s) 158, 303
BcoDI GTCTC 5 cut(s) 184, 247, 1151, 1326, 1685
BcuI ACTAGT 1 cut(s) 946
BfaI CTAG 3 cut(s) 42, 99, 947
BfmI CTRYAG 3 cut(s) 129, 803, 1529
BisI GCNGC 8 cut(s) 82, 85, 88, 116, 145, 338, 1129, 1377
BlsI GCNGC 8 cut(s) 83, 86, 89, 117, 146, 339, 1130, 1378
Bme1390I CCNGG 4 cut(s) 158, 303, 468, 1143
Bme18I GGWCC 1 cut(s) 978
BmgT120I GGNCC 5 cut(s) 19, 265, 266, 544, 978
BmiI GGNNCC 5 cut(s) 267, 307, 612, 765, 1281
BmrFI CCNGG 4 cut(s) 158, 303, 468, 1143
BmrI ACTGGG 2 cut(s) 293, 1270
BmsI GCATC 1 cut(s) 875
BmuI ACTGGG 2 cut(s) 293, 1270
BplI GAGNNNNNCTC 2 cut(s) 371, 403
BpuEI CTTGAG 2 cut(s) 1274, 1586
BpuMI CCSGG 2 cut(s) 158, 303
BsaBI GATNNNNATC 1 cut(s) 642
BsaI GGTCTC 1 cut(s) 1151
BsaJI CCNNGG 3 cut(s) 466, 1182, 1314
BsaWI WCCGGW 3 cut(s) 182, 694, 980
Bsc4I CCNNNNNNNGG 7 cut(s) 217, 275, 302, 393, 1015, 1186, 1355
Bse118I RCCGGY 1 cut(s) 980
Bse1I ACTGG 4 cut(s) 222, 288, 481, 1265
Bse3DI GCAATG 4 cut(s) 541, 858, 1083, 1584
Bse8I GATNNNNATC 1 cut(s) 642
BseAI TCCGGA 1 cut(s) 694
BseBI CCWGG 2 cut(s) 468, 1143
BseDI CCNNGG 3 cut(s) 466, 1182, 1314
BseGI GGATG 1 cut(s) 1106
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 7 cut(s) 217, 275, 302, 393, 1015, 1186, 1355
BseMI GCAATG 4 cut(s) 541, 858, 1083, 1584
BseMII CTCAG 1 cut(s) 1715
BseNI ACTGG 4 cut(s) 222, 288, 481, 1265
BseRI GAGGAG 4 cut(s) 185, 1031, 1071, 1272
BseSI GKGCMC 1 cut(s) 269
BseXI GCAGC 5 cut(s) 93, 96, 99, 324, 1388
Bsh1236I CGCG 2 cut(s) 118, 1131
Bsh1285I CGRYCG 1 cut(s) 1597
BshTI ACCGGT 1 cut(s) 980
BsiEI CGRYCG 1 cut(s) 1597
BsiHKAI GWGCWC 1 cut(s) 620
BsiSI CCGG 6 cut(s) 148, 157, 183, 303, 695, 981
BslFI GGGAC 2 cut(s) 119, 845
BslI CCNNNNNNNGG 7 cut(s) 217, 275, 302, 393, 1015, 1186, 1355
BsmAI GTCTC 5 cut(s) 184, 247, 1151, 1326, 1685
BsmBI CGTCTC 2 cut(s) 184, 247
BsmFI GGGAC 2 cut(s) 119, 845
BsmI GAATGC 3 cut(s) 568, 1031, 1483
Bso31I GGTCTC 1 cut(s) 1151
Bsp120I GGGCCC 1 cut(s) 265
Bsp1286I GDGCHC 3 cut(s) 269, 620, 766
Bsp13I TCCGGA 1 cut(s) 694
Bsp143I GATC 3 cut(s) 610, 643, 718
BspACI CCGC 8 cut(s) 116, 145, 199, 211, 823, 1129, 1265, 1597
BspCNI CTCAG 1 cut(s) 1714
BspEI TCCGGA 1 cut(s) 694
BspFNI CGCG 2 cut(s) 118, 1131
BspLI GGNNCC 5 cut(s) 267, 307, 612, 765, 1281
BspMAI CTGCAG 2 cut(s) 807, 1533
BspPI GGATC 2 cut(s) 605, 618
BspTNI GGTCTC 1 cut(s) 1151
BsrDI GCAATG 4 cut(s) 541, 858, 1083, 1584
BsrFI RCCGGY 1 cut(s) 980
BsrI ACTGG 4 cut(s) 222, 288, 481, 1265
BssAI RCCGGY 1 cut(s) 980
BssECI CCNNGG 3 cut(s) 466, 1182, 1314
BssMI GATC 3 cut(s) 610, 643, 718
BssNAI GTATAC 1 cut(s) 1651
BssSI CACGAG 2 cut(s) 323, 1036
BssT1I CCWWGG 2 cut(s) 1182, 1314
Bst1107I GTATAC 1 cut(s) 1651
Bst2BI CACGAG 2 cut(s) 323, 1036
Bst2UI CCWGG 2 cut(s) 468, 1143
Bst4CI ACNGT 3 cut(s) 133, 1393, 1713
Bst6I CTCTTC 2 cut(s) 303, 922
BstC8I GCNNGC 3 cut(s) 251, 1344, 1514
BstDEI CTNAG 1 cut(s) 1701
BstF5I GGATG 1 cut(s) 1106
BstFNI CGCG 2 cut(s) 118, 1131
BstHHI GCGC 2 cut(s) 120, 1512
BstKTI GATC 3 cut(s) 613, 646, 721
BstMAI GTCTC 5 cut(s) 184, 247, 1151, 1326, 1685
BstMBI GATC 3 cut(s) 610, 643, 718
BstMCI CGRYCG 1 cut(s) 1597
BstMWI GCNNNNNNNGC 4 cut(s) 78, 87, 343, 836
BstNI CCWGG 2 cut(s) 468, 1143
BstSCI CCNGG 4 cut(s) 156, 301, 466, 1141
BstSFI CTRYAG 3 cut(s) 129, 803, 1529
BstSLI GKGCMC 1 cut(s) 269
BstUI CGCG 2 cut(s) 118, 1131
BstV1I GCAGC 5 cut(s) 93, 96, 99, 324, 1388
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
BstZ17I GTATAC 1 cut(s) 1651
BtsCI GGATG 1 cut(s) 1106
BtsI GCAGTG 2 cut(s) 79, 139
BtsIMutI CAGTG 2 cut(s) 79, 139
Cac8I GCNNGC 3 cut(s) 251, 1344, 1514
CaiI CAGNNNCTG 1 cut(s) 446
CfoI GCGC 2 cut(s) 120, 1512
Cfr10I RCCGGY 1 cut(s) 980
Cfr13I GGNCC 5 cut(s) 19, 265, 266, 544, 978
CsiI ACCWGGT 1 cut(s) 1141
Csp6I GTAC 2 cut(s) 991, 1709
CspAI ACCGGT 1 cut(s) 980
CspCI CAANNNNNGTGG 4 cut(s) 25, 60, 358, 393
CviAII CATG 4 cut(s) 256, 671, 1024, 1537
CviQI GTAC 2 cut(s) 991, 1709
DdeI CTNAG 1 cut(s) 1701
DpnI GATC 3 cut(s) 612, 645, 720
DpnII GATC 3 cut(s) 610, 643, 718
DraIII CACNNNGTG 1 cut(s) 1430
Eam1104I CTCTTC 2 cut(s) 303, 922
EarI CTCTTC 2 cut(s) 303, 922
EciI GGCGGA 2 cut(s) 188, 1280
Eco130I CCWWGG 2 cut(s) 1182, 1314
Eco147I AGGCCT 1 cut(s) 471
Eco24I GRGCYC 2 cut(s) 269, 766
Eco31I GGTCTC 1 cut(s) 1151
Eco47I GGWCC 1 cut(s) 978
Eco57I CTGAAG 1 cut(s) 423
EcoRI GAATTC 2 cut(s) 590, 1081
EcoRII CCWGG 2 cut(s) 466, 1141
EcoT14I CCWWGG 2 cut(s) 1182, 1314
EcoT38I GRGCYC 2 cut(s) 269, 766
ErhI CCWWGG 2 cut(s) 1182, 1314
Esp3I CGTCTC 2 cut(s) 184, 247
FaeI CATG 4 cut(s) 259, 674, 1027, 1540
FalI AAGNNNNNCTT 2 cut(s) 1566, 1598
FaqI GGGAC 2 cut(s) 119, 845
FatI CATG 4 cut(s) 255, 670, 1023, 1536
FbaI TGATCA 1 cut(s) 718
FblI GTMKAC 3 cut(s) 280, 1650, 1715
Fnu4HI GCNGC 8 cut(s) 82, 85, 88, 116, 145, 338, 1129, 1377
FokI GGATG 1 cut(s) 1113
FriOI GRGCYC 2 cut(s) 269, 766
Fsp4HI GCNGC 8 cut(s) 82, 85, 88, 116, 145, 338, 1129, 1377
FspBI CTAG 3 cut(s) 42, 99, 947
GlaI GCGC 2 cut(s) 119, 1511
GluI GCNGC 8 cut(s) 82, 85, 88, 116, 145, 338, 1129, 1377
HapII CCGG 6 cut(s) 148, 157, 183, 303, 695, 981
HhaI GCGC 2 cut(s) 120, 1512
Hin1II CATG 4 cut(s) 259, 674, 1027, 1540
Hin6I GCGC 2 cut(s) 118, 1510
HinP1I GCGC 2 cut(s) 118, 1510
HindIII AAGCTT 2 cut(s) 756, 1514
HinfI GANTC 6 cut(s) 204, 399, 461, 700, 1319, 1467
HpaII CCGG 6 cut(s) 148, 157, 183, 303, 695, 981
HphI GGTGA 1 cut(s) 87
Hpy166II GTNNAC 5 cut(s) 281, 313, 1003, 1651, 1716
Hpy188I TCNGA 6 cut(s) 375, 404, 615, 705, 1464, 1704
Hpy188III TCNNGA 6 cut(s) 323, 447, 695, 1117, 1368, 1725
Hpy8I GTNNAC 5 cut(s) 281, 313, 1003, 1651, 1716
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 4 cut(s) 399, 447, 659, 755
HpyCH4III ACNGT 3 cut(s) 133, 1393, 1713
HpyCH4IV ACGT 3 cut(s) 240, 600, 993
HpyCH4V TGCA 7 cut(s) 534, 566, 805, 998, 1309, 1328, 1531
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 87, 343, 836
HpyF3I CTNAG 1 cut(s) 1701
HpySE526I ACGT 3 cut(s) 240, 600, 993
Hsp92II CATG 4 cut(s) 259, 674, 1027, 1540
HspAI GCGC 2 cut(s) 118, 1510
Kpn2I TCCGGA 1 cut(s) 694
Ksp22I TGATCA 1 cut(s) 718
Kzo9I GATC 3 cut(s) 610, 643, 718
LmnI GCTCC 5 cut(s) 159, 769, 835, 1285, 1351
Lsp1109I GCAGC 5 cut(s) 93, 96, 99, 324, 1388
LweI GCATC 1 cut(s) 875
MabI ACCWGGT 1 cut(s) 1141
MaeI CTAG 3 cut(s) 42, 99, 947
MaeII ACGT 3 cut(s) 240, 600, 993
MaeIII GTNAC 5 cut(s) 122, 320, 479, 1132, 1417
MalI GATC 3 cut(s) 612, 645, 720
MboI GATC 3 cut(s) 610, 643, 718
MboII GAAGA 7 cut(s) 182, 218, 290, 653, 909, 1356, 1635
MflI RGATCY 1 cut(s) 610
MhlI GDGCHC 3 cut(s) 269, 620, 766
MlyI GAGTC 1 cut(s) 1328
MmeI TCCRAC 2 cut(s) 1171, 1529
MroI TCCGGA 1 cut(s) 694
MroXI GAANNNNTTC 3 cut(s) 1556, 1628, 1642
MseI TTAA 4 cut(s) 498, 510, 675, 684
MslI CAYNNNNRTG 4 cut(s) 36, 260, 1036, 1541
MspA1I CMGCKG 1 cut(s) 337
MspI CCGG 6 cut(s) 148, 157, 183, 303, 695, 981
MspR9I CCNGG 4 cut(s) 158, 303, 468, 1143
Mva1269I GAATGC 3 cut(s) 568, 1031, 1483
MvaI CCWGG 2 cut(s) 468, 1143
MvnI CGCG 2 cut(s) 118, 1131
MwoI GCNNNNNNNGC 4 cut(s) 78, 87, 343, 836
NciI CCSGG 2 cut(s) 158, 303
NdeII GATC 3 cut(s) 610, 643, 718
NlaIII CATG 4 cut(s) 259, 674, 1027, 1540
NlaIV GGNNCC 5 cut(s) 267, 307, 612, 765, 1281
NmuCI GTSAC 3 cut(s) 122, 320, 1417
PceI AGGCCT 1 cut(s) 471
PctI GAATGC 3 cut(s) 568, 1031, 1483
PdmI GAANNNNTTC 3 cut(s) 1556, 1628, 1642
PfeI GAWTC 5 cut(s) 204, 399, 461, 700, 1467
PinAI ACCGGT 1 cut(s) 980
PkrI GCNGC 8 cut(s) 83, 86, 89, 117, 146, 339, 1130, 1378
PleI GAGTC 1 cut(s) 1327
PpsI GAGTC 1 cut(s) 1327
Psp1406I AACGTT 1 cut(s) 600
Psp6I CCWGG 2 cut(s) 466, 1141
PspGI CCWGG 2 cut(s) 466, 1141
PspN4I GGNNCC 5 cut(s) 267, 307, 612, 765, 1281
PspOMI GGGCCC 1 cut(s) 265
PspPI GGNCC 5 cut(s) 19, 265, 266, 544, 978
PstI CTGCAG 2 cut(s) 807, 1533
PstNI CAGNNNCTG 1 cut(s) 446
PsuI RGATCY 1 cut(s) 610
PvuII CAGCTG 1 cut(s) 337
RsaI GTAC 2 cut(s) 992, 1710
RsaNI GTAC 2 cut(s) 991, 1709
RseI CAYNNNNRTG 4 cut(s) 36, 260, 1036, 1541
SaqAI TTAA 4 cut(s) 498, 510, 675, 684
SatI GCNGC 8 cut(s) 82, 85, 88, 116, 145, 338, 1129, 1377
Sau3AI GATC 3 cut(s) 610, 643, 718
Sau96I GGNCC 5 cut(s) 19, 265, 266, 544, 978
SchI GAGTC 1 cut(s) 1328
ScrFI CCNGG 4 cut(s) 158, 303, 468, 1143
SduI GDGCHC 3 cut(s) 269, 620, 766
SexAI ACCWGGT 1 cut(s) 1141
SfaNI GCATC 1 cut(s) 875
SfcI CTRYAG 3 cut(s) 129, 803, 1529
SinI GGWCC 1 cut(s) 978
SmiMI CAYNNNNRTG 4 cut(s) 36, 260, 1036, 1541
SmlI CTYRAG 2 cut(s) 1289, 1565
SmoI CTYRAG 2 cut(s) 1289, 1565
SpeI ACTAGT 1 cut(s) 946
SseBI AGGCCT 1 cut(s) 471
SsiI CCGC 8 cut(s) 116, 145, 199, 211, 823, 1129, 1265, 1597
SspMI CTAG 3 cut(s) 42, 99, 947
StuI AGGCCT 1 cut(s) 471
StyD4I CCNGG 4 cut(s) 156, 301, 466, 1141
StyI CCWWGG 2 cut(s) 1182, 1314
TaaI ACNGT 3 cut(s) 133, 1393, 1713
TaiI ACGT 3 cut(s) 243, 603, 996
TaqI TCGA 2 cut(s) 753, 1090
TauI GCSGC 3 cut(s) 118, 147, 1131
TfiI GAWTC 5 cut(s) 204, 399, 461, 700, 1467
Tru1I TTAA 4 cut(s) 498, 510, 675, 684
Tru9I TTAA 4 cut(s) 498, 510, 675, 684
TscAI CASTG 2 cut(s) 79, 146
TseFI GTSAC 3 cut(s) 122, 320, 1417
TseI GCWGC 5 cut(s) 81, 84, 87, 337, 1376
Tsp45I GTSAC 3 cut(s) 122, 320, 1417
TspDTI ATGAA 6 cut(s) 23, 640, 654, 869, 915, 1094
TspGWI ACGGA 4 cut(s) 99, 298, 761, 998
TspRI CASTG 2 cut(s) 79, 146
VpaK11BI GGWCC 1 cut(s) 978
XapI RAATTY 5 cut(s) 590, 911, 931, 1081, 1628
XcmI CCANNNNNNNNNTGG 1 cut(s) 931
XmiI GTMKAC 3 cut(s) 280, 1650, 1715
XmnI GAANNNNTTC 3 cut(s) 1556, 1628, 1642
XspI CTAG 3 cut(s) 42, 99, 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.