pycom15g32470

Membrane-anchored ubiquitin-fold protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016524
Physical Location & Seq
Reverse (-)
31493046 .. 31495093
2048 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 360 bp
ATGGCGGCAGGTCAAGATTTGATTGAGCTGAAATTCAGGCTAATTGATGGGACCGACATAGGACCACAGAAGTATAGCCCATCTACCACTGTACAATCACTCAAGGAGAAAATACTGGCACAATGGCCTAAAGAGAAAGAAAATGGTCCAAGGACAGTAAATGATTTGAAGCTTATTAACGCGGGAAAGATACTGGAAAACAACAGGACACTTGCTGATTCCAGAAATCCAGTTGGAGAACTACCAGGAGGTCTCATCACTATGCACGTGGTTGTGCGTCCTCCGATCGCAGACAAAAAGAATGATAAGTTGATCAACGATTCTCCAGAGCAGCCTCGATGTTCATGTACAATATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rad60-SLD_2

Protein Analysis

120

Amino Acids

13.13

Weight (kDa)

8.6

Isoelectric Point (pI)

50.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD_2 PF13881 6 - 117 3.4e-44 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016524)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22050
fragaria_vesca FvH4_5g38170
malus_domestica MD08G1176300.v1.1 MD15G1362100.v1.1
prunus_persica Prupe.1G510100_v2.0.a1
pyrus_communis pycom08g15130 pycom15g32470
rosa_chinensis RchiOBHm_Chr7g0240171
rosa_laevigata RLG00000000693
rosa_multiflora Rmu_sc0005008.1_g000005
rosa_roxburghii Rroxscaffold_3G00220350
rosa_rugosa Rorug07G0333900
rosa_samantha Rh7CG504200
rosa_wichuraiana Rw7G040850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 182
AciI CCGC 2 cut(s) 5, 182
AcsI RAATTY 1 cut(s) 32
AcvI CACGTG 1 cut(s) 268
AfaI GTAC 2 cut(s) 93, 349
AgsI TTSAA 1 cut(s) 169
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 2 cut(s) 28, 172
AluI AGCT 2 cut(s) 28, 172
Alw26I GTCTC 1 cut(s) 257
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 1 cut(s) 331
ApoI RAATTY 1 cut(s) 32
AspS9I GGNCC 3 cut(s) 51, 62, 146
AvaII GGWCC 3 cut(s) 51, 62, 146
BbrPI CACGTG 1 cut(s) 268
BbvI GCAGC 1 cut(s) 343
BccI CCATC 2 cut(s) 41, 88
BciT130I CCWGG 1 cut(s) 246
BclI TGATCA 1 cut(s) 312
BcoDI GTCTC 1 cut(s) 257
BisI GCNGC 2 cut(s) 6, 332
BlsI GCNGC 2 cut(s) 7, 333
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 3 cut(s) 51, 62, 146
BmgT120I GGNCC 3 cut(s) 51, 62, 146
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 86
BsaAI YACGTR 1 cut(s) 268
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 1 cut(s) 149
Bse1I ACTGG 3 cut(s) 120, 198, 230
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 149
BseNI ACTGG 3 cut(s) 120, 198, 230
BseXI GCAGC 1 cut(s) 343
Bsh1236I CGCG 1 cut(s) 182
Bsh1285I CGRYCG 1 cut(s) 288
BshFI GGCC 1 cut(s) 127
BsiEI CGRYCG 1 cut(s) 288
BslFI GGGAC 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 64
BsnI GGCC 1 cut(s) 127
Bso31I GGTCTC 1 cut(s) 257
Bsp1407I TGTACA 2 cut(s) 91, 347
Bsp143I GATC 2 cut(s) 285, 312
BspACI CCGC 2 cut(s) 5, 182
BspANI GGCC 1 cut(s) 127
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 1 cut(s) 52
BspTNI GGTCTC 1 cut(s) 257
BsrGI TGTACA 2 cut(s) 91, 347
BsrI ACTGG 3 cut(s) 120, 198, 230
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 2 cut(s) 285, 312
BssT1I CCWWGG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 91, 157
BstAUI TGTACA 2 cut(s) 91, 347
BstBAI YACGTR 1 cut(s) 268
BstFNI CGCG 1 cut(s) 182
BstKTI GATC 2 cut(s) 288, 315
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 2 cut(s) 285, 312
BstMCI CGRYCG 1 cut(s) 288
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 244
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 343
BsuRI GGCC 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 87
Cfr13I GGNCC 3 cut(s) 51, 62, 146
CseI GACGC 1 cut(s) 266
Csp6I GTAC 2 cut(s) 92, 348
CviAII CATG 1 cut(s) 345
CviJI RGCY 6 cut(s) 28, 40, 78, 127, 172, 334
CviKI_1 RGCY 6 cut(s) 28, 40, 78, 127, 172, 334
CviQI GTAC 2 cut(s) 92, 348
DpnI GATC 2 cut(s) 287, 314
DpnII GATC 2 cut(s) 285, 312
Eco130I CCWWGG 1 cut(s) 149
Eco31I GGTCTC 1 cut(s) 257
Eco47I GGWCC 3 cut(s) 51, 62, 146
Eco72I CACGTG 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
FaeI CATG 1 cut(s) 348
FaiI YATR 4 cut(s) 59, 75, 263, 346
FaqI GGGAC 1 cut(s) 64
FatI CATG 1 cut(s) 344
FauI CCCGC 1 cut(s) 175
FbaI TGATCA 1 cut(s) 312
Fnu4HI GCNGC 2 cut(s) 6, 332
Fsp4HI GCNGC 2 cut(s) 6, 332
GluI GCNGC 2 cut(s) 6, 332
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 1 cut(s) 127
HgaI GACGC 1 cut(s) 266
Hin1II CATG 1 cut(s) 348
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 2 cut(s) 218, 320
Hpy188I TCNGA 1 cut(s) 285
Hpy188III TCNNGA 3 cut(s) 14, 222, 326
HpyCH4III ACNGT 2 cut(s) 91, 157
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 1 cut(s) 265
HpySE526I ACGT 1 cut(s) 267
Hsp92II CATG 1 cut(s) 348
Ksp22I TGATCA 1 cut(s) 312
Kzo9I GATC 2 cut(s) 285, 312
LpnPI CCDG 9 cut(s) 22, 101, 179, 190, 231, 235, 243, 258, 339
Lsp1109I GCAGC 1 cut(s) 343
MaeII ACGT 1 cut(s) 267
MalI GATC 2 cut(s) 287, 314
MboI GATC 2 cut(s) 285, 312
MluCI AATT 2 cut(s) 32, 42
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 3 cut(s) 242, 291, 345
MseI TTAA 1 cut(s) 177
MslI CAYNNNNRTG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 246
MvnI CGCG 1 cut(s) 182
NdeII GATC 2 cut(s) 285, 312
NlaIII CATG 1 cut(s) 348
NlaIV GGNNCC 1 cut(s) 52
PfeI GAWTC 2 cut(s) 218, 320
PkrI GCNGC 2 cut(s) 7, 333
Ple19I CGATCG 1 cut(s) 288
PmaCI CACGTG 1 cut(s) 268
PmlI CACGTG 1 cut(s) 268
Ppu21I YACGTR 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 244
PspCI CACGTG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 3 cut(s) 51, 62, 146
PvuI CGATCG 1 cut(s) 288
RsaI GTAC 2 cut(s) 93, 349
RsaNI GTAC 2 cut(s) 92, 348
RseI CAYNNNNRTG 1 cut(s) 260
SaqAI TTAA 1 cut(s) 177
SatI GCNGC 2 cut(s) 6, 332
Sau3AI GATC 2 cut(s) 285, 312
Sau96I GGNCC 3 cut(s) 51, 62, 146
ScrFI CCNGG 1 cut(s) 246
SetI ASST 5 cut(s) 13, 30, 174, 253, 270
SinI GGWCC 3 cut(s) 51, 62, 146
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 2 cut(s) 32, 42
SsiI CCGC 2 cut(s) 5, 182
SspI AATATT 1 cut(s) 354
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 2 cut(s) 91, 157
TaiI ACGT 1 cut(s) 270
TaqI TCGA 1 cut(s) 337
TaqII GACCGA 1 cut(s) 68
TasI AATT 2 cut(s) 32, 42
TatI WGTACW 2 cut(s) 91, 347
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 218, 320
Tru1I TTAA 1 cut(s) 177
Tru9I TTAA 1 cut(s) 177
TscAI CASTG 1 cut(s) 94
TseI GCWGC 1 cut(s) 331
TspDTI ATGAA 1 cut(s) 333
TspRI CASTG 1 cut(s) 94
VpaK11BI GGWCC 3 cut(s) 51, 62, 146
XapI RAATTY 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.