pycom15g34130

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012089
Physical Location & Seq
Forward (+)
33705424 .. 33709901
4478 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1071 bp
ATGCTCAAGACTCAAAGAACACTGTCCAAATCCAAGATCCCAAATAAAACGCTCCTCCCCTCAAAACCAAACCAAATCTGGTCGCACCAGTTGTCTTCTCGCAGACGAACAAGCCATTTGAATTCCCAAACAAGACAAAGATTCCATTTTCCCAATCACTACAATATGAGCGACCACAAGGCGGCAGCTGCACCACCTGCACCTGATCACCCACCACCACCATCTCACTCCCAAGACTACGCTCCATACCCTAAGCTTGACCTCAATGACGTCGCTCCGCCTCCCCACTCCGAAACTTGGACCTCCGTCTCCATCGCCTCTGAACCGCCGAAATCGGAGGGTCCGCCGGTGCAGCCGCCTGTGTCTCCACCGGTGTCCGATCCGGTGGTTCACGCCGAGGCTCGCGGTCCGATCCCCGGCGACGCCGCCACCTCCATGCCGGCGGAGTCCAATCCCTACGTTGCCACTCCTGCGCCGCCTCCCCCCTCCTCCGTCAAGAAATCGATGGATTCAGTGAAAGATGCGCTTGGAAAATGGGGAAAGAAGGCTGCGGAGGCCACCAAGAAGGCTGAGGATCTTGCTGGCAACATGTGGCAACACTTGAAGACAGGACCAAGTTTTGCTGATGCTGCTGTGACAAGAATTGCTCAGAGCACTAAAGTACTGGCAGAAGGTGGTTATGAGAAGATCTTTCGACAAAATTTTGAGACTGTCCCAGAGGAGCAACTTCGAAAGACATTTGCTTGCTACTTATCTACATCTGCTGGTCCAGTAATGGGAACCCTATTTTTGTCATCAGCAAAACTTGCATTTTGCAGTGACGATCCTCTTTCATACAAAGTAGGTGACGGCACTGAATGGAGCTATTATAAGGTGATTATTCCGTTACATCAACTGAAGGCAGTCAATCCATCAACGAGCAAAGTGAAAGCAGCTGAAAAGTACATCCAGGTCGTCTCTGTTGACAACCATGAGTTTTGGTTCATGAGCTTTGTGCACTACGATAGTGCTGTGAAACATCTTCAAGAAGTAGTGCAGCCTCACCCTCAGAGTGAGCCCCAGAGTGCATAA

Protein Analysis

357

Amino Acids

39.03

Weight (kDa)

8.94

Isoelectric Point (pI)

54.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 230 - 346 3.8e-19 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28200 AT1G28200
fragaria_vesca FvH4_5g35910
malus_domestica MD08G1192100.v1.1 MD15G1380800.v1.1
prunus_persica Prupe.1G527000_v2.0.a1
pyrus_communis pycom08g16590 pycom15g34130
rosa_chinensis RchiOBHm_Chr7g0237641
rosa_laevigata RLG00000000979
rosa_multiflora Rmu_sc0007826.1_g000010
rosa_roxburghii Rroxscaffold_3G00223910
rosa_rugosa Rorug07G0305800 Rorug07G0305900
rosa_samantha Rh7AG462600 Rh7BG432600 Rh7CG479400 Rh7DG448400
rosa_wichuraiana Rw7G038270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 870
AarI CACCTGC 1 cut(s) 205
AatII GACGTC 1 cut(s) 273
Acc36I ACCTGC 1 cut(s) 205
AccII CGCG 1 cut(s) 405
AclWI GGATC 5 cut(s) 31, 374, 406, 582, 818
AcsI RAATTY 2 cut(s) 121, 700
AcuI CTGAAG 1 cut(s) 917
AcyI GRCGYC 2 cut(s) 270, 423
AfaI GTAC 2 cut(s) 663, 944
AfiI CCNNNNNNNGG 4 cut(s) 181, 297, 416, 776
AflIII ACRYGT 1 cut(s) 588
AgeI ACCGGT 1 cut(s) 370
AgsI TTSAA 3 cut(s) 121, 604, 1025
AjnI CCWGG 1 cut(s) 948
AluBI AGCT 5 cut(s) 188, 256, 864, 935, 990
AluI AGCT 5 cut(s) 188, 256, 864, 935, 990
Alw21I GWGCWC 2 cut(s) 656, 999
Alw26I GTCTC 4 cut(s) 313, 369, 701, 961
Alw44I GTGCAC 1 cut(s) 995
AlwI GGATC 5 cut(s) 31, 374, 406, 582, 818
AoxI GGCC 1 cut(s) 555
ApaLI GTGCAC 1 cut(s) 995
ApeKI GCWGC 7 cut(s) 185, 188, 352, 548, 629, 932, 1036
ApoI RAATTY 2 cut(s) 121, 700
AsiGI ACCGGT 1 cut(s) 370
AspLEI GCGC 2 cut(s) 475, 526
AspS9I GGNCC 5 cut(s) 300, 341, 407, 611, 767
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 4 cut(s) 200, 857, 886, 1034
AsuII TTCGAA 1 cut(s) 730
AvaII GGWCC 5 cut(s) 300, 341, 407, 611, 767
BaeGI GKGCMC 1 cut(s) 999
BanII GRGCYC 1 cut(s) 1059
BbsI GAAGAC 2 cut(s) 87, 611
Bbv12I GWGCWC 2 cut(s) 656, 999
BbvCI CCTCAGC 1 cut(s) 570
BbvI GCAGC 7 cut(s) 175, 197, 364, 535, 616, 944, 1048
BccI CCATC 4 cut(s) 229, 320, 499, 919
BceAI ACGGC 1 cut(s) 865
BciT130I CCWGG 1 cut(s) 950
BclI TGATCA 1 cut(s) 205
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 4 cut(s) 313, 369, 701, 961
BfuAI ACCTGC 1 cut(s) 205
BglII AGATCT 1 cut(s) 687
BmcAI AGTACT 1 cut(s) 663
Bme1390I CCNGG 2 cut(s) 417, 950
Bme18I GGWCC 5 cut(s) 300, 341, 407, 611, 767
BmgT120I GGNCC 5 cut(s) 300, 341, 407, 611, 767
BmiI GGNNCC 2 cut(s) 342, 781
BmrFI CCNGG 2 cut(s) 417, 950
BmsI GCATC 2 cut(s) 511, 616
BoxI GACNNNNGTC 1 cut(s) 305
BpiI GAAGAC 2 cut(s) 87, 611
Bpu10I CCTNAGC 2 cut(s) 252, 570
Bpu14I TTCGAA 1 cut(s) 730
BpuMI CCSGG 1 cut(s) 417
Bsa29I ATCGAT 1 cut(s) 503
BsaHI GRCGYC 2 cut(s) 270, 423
BsaJI CCNNGG 2 cut(s) 396, 415
BsaWI WCCGGW 2 cut(s) 370, 382
Bsc4I CCNNNNNNNGG 4 cut(s) 181, 297, 416, 776
Bse118I RCCGGY 3 cut(s) 346, 370, 439
Bse1I ACTGG 3 cut(s) 88, 669, 770
BseBI CCWGG 1 cut(s) 950
BseCI ATCGAT 1 cut(s) 503
BseDI CCNNGG 2 cut(s) 396, 415
BseGI GGATG 1 cut(s) 945
BseLI CCNNNNNNNGG 4 cut(s) 181, 297, 416, 776
BseMII CTCAG 3 cut(s) 561, 662, 1061
BseNI ACTGG 3 cut(s) 88, 669, 770
BseRI GAGGAG 3 cut(s) 44, 478, 734
BseSI GKGCMC 1 cut(s) 999
BseXI GCAGC 7 cut(s) 175, 197, 364, 535, 616, 944, 1048
BsgI GTGCAG 4 cut(s) 174, 183, 371, 1055
Bsh1236I CGCG 1 cut(s) 405
BshFI GGCC 1 cut(s) 557
BshTI ACCGGT 1 cut(s) 370
BshVI ATCGAT 1 cut(s) 503
BsiHKAI GWGCWC 2 cut(s) 656, 999
BsiSI CCGG 5 cut(s) 347, 371, 383, 417, 440
BslFI GGGAC 1 cut(s) 698
BslI CCNNNNNNNGG 4 cut(s) 181, 297, 416, 776
BsmAI GTCTC 4 cut(s) 313, 369, 701, 961
BsmBI CGTCTC 2 cut(s) 313, 961
BsmFI GGGAC 1 cut(s) 698
BsnI GGCC 1 cut(s) 557
Bsp119I TTCGAA 1 cut(s) 730
Bsp1286I GDGCHC 3 cut(s) 656, 999, 1059
Bsp143I GATC 7 cut(s) 36, 205, 379, 411, 574, 687, 823
BspANI GGCC 1 cut(s) 557
BspCNI CTCAG 3 cut(s) 562, 661, 1060
BspDI ATCGAT 1 cut(s) 503
BspFNI CGCG 1 cut(s) 405
BspHI TCATGA 1 cut(s) 984
BspLI GGNNCC 2 cut(s) 342, 781
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 5 cut(s) 31, 374, 406, 582, 818
BspT104I TTCGAA 1 cut(s) 730
BsrFI RCCGGY 3 cut(s) 346, 370, 439
BsrI ACTGG 3 cut(s) 88, 669, 770
BssAI RCCGGY 3 cut(s) 346, 370, 439
BssECI CCNNGG 2 cut(s) 396, 415
BssMI GATC 7 cut(s) 36, 205, 379, 411, 574, 687, 823
BssNI GRCGYC 2 cut(s) 270, 423
Bst2UI CCWGG 1 cut(s) 950
Bst4CI ACNGT 2 cut(s) 24, 712
BstACI GRCGYC 2 cut(s) 270, 423
BstAPI GCANNNNNTGC 2 cut(s) 197, 806
BstBI TTCGAA 1 cut(s) 730
BstC8I GCNNGC 4 cut(s) 403, 441, 583, 745
BstDEI CTNAG 4 cut(s) 252, 570, 648, 1047
BstF5I GGATG 1 cut(s) 945
BstFNI CGCG 1 cut(s) 405
BstHHI GCGC 2 cut(s) 475, 526
BstKTI GATC 7 cut(s) 39, 208, 382, 414, 577, 690, 826
BstMAI GTCTC 4 cut(s) 313, 369, 701, 961
BstMBI GATC 7 cut(s) 36, 205, 379, 411, 574, 687, 823
BstMWI GCNNNNNNNGC 7 cut(s) 188, 197, 352, 470, 554, 629, 806
BstNI CCWGG 1 cut(s) 950
BstNSI RCATGY 1 cut(s) 592
BstPAI GACNNNNGTC 1 cut(s) 305
BstSCI CCNGG 2 cut(s) 415, 948
BstSLI GKGCMC 1 cut(s) 999
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 7 cut(s) 175, 197, 364, 535, 616, 944, 1048
BstV2I GAAGAC 2 cut(s) 87, 611
BstX2I RGATCY 3 cut(s) 36, 574, 687
BstYI RGATCY 3 cut(s) 36, 574, 687
Bsu15I ATCGAT 1 cut(s) 503
BsuRI GGCC 1 cut(s) 557
BsuTUI ATCGAT 1 cut(s) 503
BtgZI GCGATG 1 cut(s) 298
BtsCI GGATG 1 cut(s) 945
BtsI GCAGTG 1 cut(s) 823
BtsIMutI CAGTG 4 cut(s) 20, 519, 823, 852
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 4 cut(s) 403, 441, 583, 745
CciI TCATGA 1 cut(s) 984
CfoI GCGC 2 cut(s) 475, 526
Cfr10I RCCGGY 3 cut(s) 346, 370, 439
Cfr13I GGNCC 5 cut(s) 300, 341, 407, 611, 767
ClaI ATCGAT 1 cut(s) 503
CpoI CGGWCCG 1 cut(s) 407
CseI GACGC 1 cut(s) 431
Csp6I GTAC 2 cut(s) 662, 943
CspAI ACCGGT 1 cut(s) 370
CspI CGGWCCG 1 cut(s) 407
CviAII CATG 4 cut(s) 436, 589, 971, 985
CviQI GTAC 2 cut(s) 662, 943
DdeI CTNAG 4 cut(s) 252, 570, 648, 1047
DpnI GATC 7 cut(s) 38, 207, 381, 413, 576, 689, 825
DpnII GATC 7 cut(s) 36, 205, 379, 411, 574, 687, 823
EciI GGCGGA 3 cut(s) 267, 333, 458
Eco24I GRGCYC 1 cut(s) 1059
Eco47I GGWCC 5 cut(s) 300, 341, 407, 611, 767
Eco57I CTGAAG 1 cut(s) 917
EcoRI GAATTC 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 948
EcoT38I GRGCYC 1 cut(s) 1059
Esp3I CGTCTC 2 cut(s) 313, 961
FaeI CATG 4 cut(s) 439, 592, 974, 988
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FaqI GGGAC 1 cut(s) 698
FatI CATG 4 cut(s) 435, 588, 970, 984
FbaI TGATCA 1 cut(s) 205
FokI GGATG 1 cut(s) 932
FriOI GRGCYC 1 cut(s) 1059
GlaI GCGC 2 cut(s) 474, 525
HaeIII GGCC 1 cut(s) 557
HapII CCGG 5 cut(s) 347, 371, 383, 417, 440
HgaI GACGC 1 cut(s) 431
HhaI GCGC 2 cut(s) 475, 526
Hin1I GRCGYC 2 cut(s) 270, 423
Hin1II CATG 4 cut(s) 439, 592, 974, 988
Hin6I GCGC 2 cut(s) 473, 524
HinP1I GCGC 2 cut(s) 473, 524
HincII GTYRAC 1 cut(s) 964
HindII GTYRAC 1 cut(s) 964
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 4 cut(s) 10, 141, 446, 509
HpaII CCGG 5 cut(s) 347, 371, 383, 417, 440
HphI GGTGA 4 cut(s) 200, 857, 886, 1034
Hpy166II GTNNAC 3 cut(s) 391, 964, 997
Hpy188I TCNGA 7 cut(s) 292, 322, 337, 379, 411, 651, 1050
Hpy188III TCNNGA 4 cut(s) 7, 496, 985, 1025
Hpy8I GTNNAC 3 cut(s) 391, 964, 997
Hpy99I CGWCG 2 cut(s) 275, 425
HpyAV CCTTC 4 cut(s) 538, 559, 665, 892
HpyCH4III ACNGT 2 cut(s) 24, 712
HpyCH4IV ACGT 2 cut(s) 270, 459
HpyCH4V TGCA 8 cut(s) 191, 200, 352, 809, 816, 997, 1036, 1067
HpyF10VI GCNNNNNNNGC 7 cut(s) 188, 197, 352, 470, 554, 629, 806
HpyF3I CTNAG 4 cut(s) 252, 570, 648, 1047
HpySE526I ACGT 2 cut(s) 270, 459
Hsp92I GRCGYC 2 cut(s) 270, 423
Hsp92II CATG 4 cut(s) 439, 592, 974, 988
HspAI GCGC 2 cut(s) 473, 524
KroI GCCGGC 1 cut(s) 439
KroNI GCCGGC 1 cut(s) 441
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 7 cut(s) 36, 205, 379, 411, 574, 687, 823
LmnI GCTCC 5 cut(s) 57, 247, 280, 721, 861
Lsp1109I GCAGC 7 cut(s) 175, 197, 364, 535, 616, 944, 1048
LweI GCATC 2 cut(s) 511, 616
MaeII ACGT 2 cut(s) 270, 459
MaeIII GTNAC 4 cut(s) 634, 818, 845, 885
MalI GATC 7 cut(s) 38, 207, 381, 413, 576, 689, 825
MboI GATC 7 cut(s) 36, 205, 379, 411, 574, 687, 823
MboII GAAGA 4 cut(s) 87, 616, 697, 1013
MflI RGATCY 3 cut(s) 36, 574, 687
MhlI GDGCHC 3 cut(s) 656, 999, 1059
MluCI AATT 3 cut(s) 121, 642, 700
MlyI GAGTC 2 cut(s) 4, 455
MroNI GCCGGC 1 cut(s) 439
MslI CAYNNNNRTG 1 cut(s) 434
MspA1I CMGCKG 2 cut(s) 188, 935
MspI CCGG 5 cut(s) 347, 371, 383, 417, 440
MspR9I CCNGG 2 cut(s) 417, 950
MvaI CCWGG 1 cut(s) 950
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 7 cut(s) 188, 197, 352, 470, 554, 629, 806
NaeI GCCGGC 1 cut(s) 441
NciI CCSGG 1 cut(s) 417
NdeII GATC 7 cut(s) 36, 205, 379, 411, 574, 687, 823
NgoMIV GCCGGC 1 cut(s) 439
NlaIII CATG 4 cut(s) 439, 592, 974, 988
NlaIV GGNNCC 2 cut(s) 342, 781
NmeAIII GCCGAG 1 cut(s) 421
NmuCI GTSAC 3 cut(s) 634, 818, 845
NspI RCATGY 1 cut(s) 592
NspV TTCGAA 1 cut(s) 730
PagI TCATGA 1 cut(s) 984
PaqCI CACCTGC 1 cut(s) 205
PciI ACATGT 1 cut(s) 588
PdiI GCCGGC 1 cut(s) 441
PfeI GAWTC 2 cut(s) 141, 509
PinAI ACCGGT 1 cut(s) 370
PleI GAGTC 2 cut(s) 4, 454
PpsI GAGTC 2 cut(s) 4, 454
PscI ACATGT 1 cut(s) 588
PshAI GACNNNNGTC 1 cut(s) 305
PsiI TTATAA 1 cut(s) 870
Psp6I CCWGG 1 cut(s) 948
PspGI CCWGG 1 cut(s) 948
PspN4I GGNNCC 2 cut(s) 342, 781
PspPI GGNCC 5 cut(s) 300, 341, 407, 611, 767
PsuI RGATCY 3 cut(s) 36, 574, 687
PvuII CAGCTG 2 cut(s) 188, 935
RsaI GTAC 2 cut(s) 663, 944
RsaNI GTAC 2 cut(s) 662, 943
RseI CAYNNNNRTG 1 cut(s) 434
Rsr2I CGGWCCG 1 cut(s) 407
RsrII CGGWCCG 1 cut(s) 407
Sau3AI GATC 7 cut(s) 36, 205, 379, 411, 574, 687, 823
Sau96I GGNCC 5 cut(s) 300, 341, 407, 611, 767
ScaI AGTACT 1 cut(s) 663
SchI GAGTC 2 cut(s) 4, 455
ScrFI CCNGG 2 cut(s) 417, 950
SduI GDGCHC 3 cut(s) 656, 999, 1059
SfaNI GCATC 2 cut(s) 511, 616
SfuI TTCGAA 1 cut(s) 730
SgrAI CRCCGGYG 2 cut(s) 346, 370
SinI GGWCC 5 cut(s) 300, 341, 407, 611, 767
SmiMI CAYNNNNRTG 1 cut(s) 434
SmlI CTYRAG 1 cut(s) 5
SmoI CTYRAG 1 cut(s) 5
Sse9I AATT 3 cut(s) 121, 642, 700
StyD4I CCNGG 2 cut(s) 415, 948
TaaI ACNGT 2 cut(s) 24, 712
TaiI ACGT 2 cut(s) 273, 462
TaqI TCGA 3 cut(s) 503, 694, 730
TasI AATT 3 cut(s) 121, 642, 700
TatI WGTACW 2 cut(s) 661, 942
TauI GCSGC 4 cut(s) 185, 358, 428, 478
TfiI GAWTC 2 cut(s) 141, 509
TscAI CASTG 4 cut(s) 27, 519, 823, 859
TseFI GTSAC 3 cut(s) 634, 818, 845
TseI GCWGC 7 cut(s) 185, 188, 352, 548, 629, 932, 1036
Tsp45I GTSAC 3 cut(s) 634, 818, 845
TspDTI ATGAA 2 cut(s) 822, 973
TspGWI ACGGA 3 cut(s) 295, 481, 873
TspRI CASTG 4 cut(s) 27, 519, 823, 859
VneI GTGCAC 1 cut(s) 995
VpaK11BI GGWCC 5 cut(s) 300, 341, 407, 611, 767
XapI RAATTY 2 cut(s) 121, 700
XceI RCATGY 1 cut(s) 592
XcmI CCANNNNNNNNNTGG 1 cut(s) 75
ZraI GACGTC 1 cut(s) 271
ZrmI AGTACT 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.