pycom15g34170

WD and tetratricopeptide repeats protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007919
Physical Location & Seq
Reverse (-)
33790820 .. 33801884
11065 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2298 bp
ATGGAAGGCGTCCCTTTTCACGACGGCAACATCCACGACTTGATTGCAAGACGATACGTCGACGTTCGGCAAGATGCCAATCACAGGTTGCAGATGCACTCGTCGTTGGTGCGAAGGCTTTCTCAGGAAAGAGAATTGGAGGGGCATCAAGGTTGTGTTAATTCCATTGCTTGGAATTCGAATGGCTCGCTTTTAATGTCTGGATCAGATGACACACGGATAAATATCTGGAGCTACTCTAGTCGGAAGCTTTTGCATTCTATAGAGACTGGGCACAGTGCGAACATATTTTGTACAAAATTTGTCCCTGAAACTTCTGATGAGCTTGTGGTCTCTGGAGCTGGAGATGCAGAAGTTCGATTATTTAATTTGTCTCGTTTAAGTGGGAGAGGAACTGATGATAATGCTATTTCTCCATCAGCTGTGTATCAATGCCATAGTAGAAGAGTAAAAAAGTTAGCTGTTGAAGTTGGAAATCCCAATGTGGTATGGAGTGCAAGTGAAGATGGAACTTTGAGACAGCATGACTTCCGAGAGGGCACTTCTTGCCCACCTGCTGGATCCACTAATCAAGAATGTCGCAATGTTTTGCTTGACTTGCGGTGTGGAGCAAAGAAGTCACTTGCTGATCCCCCCAAGCAAAATTTTTCTTTAAAATCTTGTGATATCAGTTCCACTAGACCTCATTTGCTCCTAGTTGGTGGGAGTGATGCATTTGCCCGTTTATATGATAGAAGGATGCTGCCACCACTGACATCCTGTCGGAAACGGAGAGCACCACCTCCTTGTGTTAACTATTTTTGTCCAATGCATCTCTCCGAACGTGGACGTGCTAGCTTGCATTTGACTCATGTTGCATTTAGTCCAGACGGAGAAGAGGTTCTACTTAGTTATAGTGGGGAGCACATATATTTGATGAATGTAAATCATGCTGGTGAGAGTGCTATGCAGTATACTTCTGGAGATGTTTTGAAACTAACCAGCTTCAGTCCTATACTCAATGGAGTCGAACTGCATCAACCAGCATCCAATGTGTTCAGAAATGGTCTTCCCAAAAGAGGCAACGTTGCTGCTAGGTTTGAGAAGTGCAGGAATCTAATTGAAATTGCTGAGCGGTCCTTGGAGGAGGGGACAGATTATTTTTATGGCATTGAAGCGTGCAATGAGGTGTTGGATGGAAATGGTTGTGATATTGGGCCTACACTAAAGCATGATTGCCTTTGTATTCGTGCGGCATTGTTGCTTAAGCGCCAGTGGAAAAATGATGTTCATATGGCTATAAGAGATTGTTATAATGCTCGGAGGCTCATTAGCTCCTCTTACAGAGCTCATTACTATATGTCTGAAGCTTTATCGCAGTTGGCTAAACATAAAGAAGCTCTGGAATTTGCTATCGCAGCCCAATCTTTGGCTCCATCAAATTCTCAAGTAGCAGAACGATTGGAGCGTGTACAAGGGGATCTTGCTGCAGTTGAATCTGAAAGAAATAGTAAGCCAAATGATGGAGCACTGAGGTCTGAACCTCGAGGTGAAAGAGTTCTATCACTCAGTGACATACTCTACCGATCAGAGGGTAATAGTGATGTTTCACAAGATGGTCCAAGATCTGAAAGAGAAGATTCTGATTATGATGAGGAACTAGAGCTGGACTTTGAAACGTCGATCTCAGGTGATGAAGAGCATGATATCGAGCCCAATATTGTACAAGGAAGCTTAAACTTGAGAATTCATCGAAGAAGTGATTCTGCTAGAGAAGTTGGTGGTGCAAACGGCCCATCTGGATCCCCCTCTTCGTCATCTCAAAATGAAAGAATACCATATCAGCCTGAGGCAGTTATTGATATGAAACAGAGATATGTTGGCCATTGTAATGTTGGAACGGACATAAAACAGGCCAGCTTTCTTGGCCAGCGAGGTGAATATGTTGCTAGCGGAAGTGATGATGGCAGATGGTTTGTCTGGGAAAAGCGAACTGGTAGACTGATAAAAATGCTTCAAGGAGATGAAACTGTTGTGAACTGTGTACAGTGCCATCCGGTTGATTGTGTTGTGGCAACTAGTGGAATTGACAACACAATAAAAATTTGGACCCCCAGTGCCTCAGTCCCATCTATTGTGGCTGGTGGAGCCGCTGGACCAGAAAATGCTGACATTTCGTTAGTCATGGAATCCAATCAGCGCAGATTAAGCCATAATCGTGAGACTATCCTGTACAGCCATTCTGAAATTTTGGAGCATTTTCGAATGCACGAGTTTGCTGAAGGAAGCTTGCACCCATTTGAGTGTGCCCAGAGCTGA

Protein Analysis

766

Amino Acids

84.83

Weight (kDa)

5.87

Isoelectric Point (pI)

55.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 26 - 125 1.1e-10 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 32 - 125 1.3e-07 TEP-1 second beta-propeller
ANAPC4_WD40 PF12894 37 - 99 7.5e-06 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR5 PF25175 38 - 181 2.2e-19 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 41 - 125 2.3e-13 WDR3 first beta-propeller domain
WDR55 PF24796 42 - 180 3.1e-07 WDR55
WD40 PF00400 43 - 78 3.9e-09 WD domain, G-beta repeat
EIF3I PF24805 46 - 125 8.4e-07 EIF3I
Beta-prop_THOC3 PF25174 49 - 125 4.7e-12 THOC3 beta-propeller domain
WD40_Prp19 PF24814 90 - 182 4.7e-06 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 615 - 697 6.5e-13 WDR5 beta-propeller domain
WD40_Prp19 PF24814 631 - 701 4.1e-07 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 637 - 697 5.4e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 638 - 698 1.9e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1293
AarI CACCTGC 1 cut(s) 562
AbsI CCTCGAGG 1 cut(s) 1524
Acc36I ACCTGC 1 cut(s) 562
AccB7I CCANNNNNTGG 4 cut(s) 171, 557, 1408, 1502
AccBSI CCGCTC 1 cut(s) 1114
AccI GTMKAC 3 cut(s) 60, 953, 1978
AciI CCGC 5 cut(s) 601, 1114, 1232, 1932, 2130
AclI AACGTT 1 cut(s) 1065
AclWI GGATC 7 cut(s) 211, 555, 568, 623, 1467, 1776, 1789
AcoI YGGCCR 2 cut(s) 1861, 1906
AcsI RAATTY 8 cut(s) 175, 299, 643, 1385, 1420, 1725, 2082, 2226
AcuI CTGAAG 3 cut(s) 970, 1365, 2280
AcyI GRCGYC 1 cut(s) 9
AdeI CACNNNGTG 1 cut(s) 1550
AfaI GTAC 5 cut(s) 295, 1452, 1704, 2025, 2213
AfiI CCNNNNNNNGG 7 cut(s) 84, 171, 557, 1058, 1408, 1502, 1570
AflII CTTAAG 1 cut(s) 1244
AgsI TTSAA 7 cut(s) 467, 973, 1103, 1154, 1475, 1655, 1997
AhdI GACNNNNNGTC 2 cut(s) 56, 759
AhlI ACTAGT 1 cut(s) 2057
AjiI CACGTC 1 cut(s) 830
AloI GAACNNNNNNTCC 2 cut(s) 864, 896
Alw21I GWGCWC 4 cut(s) 778, 906, 1330, 1510
Alw26I GTCTC 5 cut(s) 260, 337, 378, 511, 2195
AlwI GGATC 7 cut(s) 211, 555, 568, 623, 1467, 1776, 1789
Ama87I CYCGRG 1 cut(s) 1524
AoxI GGCC 5 cut(s) 1196, 1771, 1861, 1893, 1906
ApeKI GCWGC 4 cut(s) 742, 1070, 1397, 1466
ApoI RAATTY 8 cut(s) 175, 299, 643, 1385, 1420, 1725, 2082, 2226
Asp700I GAANNNNTTC 4 cut(s) 118, 879, 1536, 1741
AspLEI GCGC 2 cut(s) 1251, 2181
AspS9I GGNCC 6 cut(s) 1116, 1196, 1598, 1772, 2088, 2135
AsuHPI GGTGA 4 cut(s) 947, 1541, 1682, 1928
AsuII TTCGAA 2 cut(s) 179, 2242
AsuNHI GCTAGC 2 cut(s) 833, 1928
AvaI CYCGRG 1 cut(s) 1524
AvaII GGWCC 4 cut(s) 1116, 1598, 2088, 2135
AxyI CCTNAGG 1 cut(s) 1827
BaeGI GKGCMC 3 cut(s) 276, 542, 2290
BalI TGGCCA 2 cut(s) 1863, 1908
BamHI GGATCC 2 cut(s) 560, 1781
BanII GRGCYC 2 cut(s) 1330, 1695
BarI GAAGNNNNNNTAC 2 cut(s) 867, 899
BauI CACGAG 1 cut(s) 2249
BbsI GAAGAC 1 cut(s) 1040
Bbv12I GWGCWC 4 cut(s) 778, 906, 1330, 1510
BbvI GCAGC 4 cut(s) 729, 1057, 1409, 1453
BceAI ACGGC 2 cut(s) 40, 1786
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BcoDI GTCTC 5 cut(s) 260, 337, 378, 511, 2195
BcuI ACTAGT 1 cut(s) 2057
BfaI CTAG 9 cut(s) 240, 678, 695, 834, 1074, 1640, 1749, 1929, 2058
BfmI CTRYAG 2 cut(s) 261, 1467
BfoI RGCGCY 1 cut(s) 1252
BfrI CTTAAG 1 cut(s) 1244
BfuAI ACCTGC 1 cut(s) 562
BglII AGATCT 1 cut(s) 1604
BisI GCNGC 6 cut(s) 743, 1071, 1233, 1398, 1467, 2130
BlpI GCTNAGC 1 cut(s) 1110
BlsI GCNGC 6 cut(s) 744, 1072, 1234, 1399, 1468, 2131
Bme18I GGWCC 4 cut(s) 1116, 1598, 2088, 2135
BmeRI GACNNNNNGTC 2 cut(s) 56, 759
BmeT110I CYCGRG 1 cut(s) 1524
BmgBI CACGTC 1 cut(s) 830
BmgT120I GGNCC 6 cut(s) 1116, 1196, 1598, 1772, 2088, 2135
BmiI GGNNCC 5 cut(s) 562, 1413, 1783, 2090, 2128
BmrI ACTGGG 2 cut(s) 279, 2088
BmsI GCATC 9 cut(s) 64, 84, 154, 337, 700, 729, 820, 1024, 1034
BmtI GCTAGC 2 cut(s) 837, 1932
BmuI ACTGGG 2 cut(s) 279, 2088
BpiI GAAGAC 1 cut(s) 1040
BpmI CTGGAG 4 cut(s) 250, 357, 363, 981
Bpu1102I GCTNAGC 1 cut(s) 1110
Bpu14I TTCGAA 2 cut(s) 179, 2242
BpuEI CTTGAG 2 cut(s) 1410, 1741
BsaBI GATNNNNATC 2 cut(s) 78, 224
BsaHI GRCGYC 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 337
BsaJI CCNNGG 1 cut(s) 1119
BsaWI WCCGGW 1 cut(s) 2035
BsaXI ACNNNNNCTCC 4 cut(s) 484, 514, 864, 894
Bsc4I CCNNNNNNNGG 7 cut(s) 84, 171, 557, 1058, 1408, 1502, 1570
Bse1I ACTGG 4 cut(s) 274, 1252, 1978, 2094
Bse21I CCTNAGG 1 cut(s) 1827
Bse3DI GCAATG 3 cut(s) 165, 589, 1168
Bse8I GATNNNNATC 2 cut(s) 78, 224
BseDI CCNNGG 1 cut(s) 1119
BseGI GGATG 6 cut(s) 30, 744, 755, 1025, 1180, 2032
BseJI GATNNNNATC 2 cut(s) 78, 224
BseLI CCNNNNNNNGG 7 cut(s) 84, 171, 557, 1058, 1408, 1502, 1570
BseMI GCAATG 3 cut(s) 165, 589, 1168
BseMII CTCAG 7 cut(s) 137, 1101, 1502, 1561, 1680, 1818, 2115
BseNI ACTGG 4 cut(s) 274, 1252, 1978, 2094
BseRI GAGGAG 2 cut(s) 1139, 1306
BseSI GKGCMC 3 cut(s) 276, 542, 2290
BseXI GCAGC 4 cut(s) 729, 1057, 1409, 1453
BsgI GTGCAG 1 cut(s) 1108
BshFI GGCC 5 cut(s) 1198, 1773, 1863, 1895, 1908
BsiHKAI GWGCWC 4 cut(s) 778, 906, 1330, 1510
BsiHKCI CYCGRG 1 cut(s) 1524
BsiSI CCGG 1 cut(s) 2036
BslFI GGGAC 3 cut(s) 290, 1144, 2090
BslI CCNNNNNNNGG 7 cut(s) 84, 171, 557, 1058, 1408, 1502, 1570
BsmAI GTCTC 5 cut(s) 260, 337, 378, 511, 2195
BsmFI GGGAC 3 cut(s) 290, 1144, 2090
BsmI GAATGC 2 cut(s) 256, 2250
BsnI GGCC 5 cut(s) 1198, 1773, 1863, 1895, 1908
Bso31I GGTCTC 1 cut(s) 337
BsoBI CYCGRG 1 cut(s) 1524
Bsp119I TTCGAA 2 cut(s) 179, 2242
Bsp1286I GDGCHC 8 cut(s) 276, 542, 778, 906, 1330, 1510, 1695, 2290
Bsp1407I TGTACA 5 cut(s) 293, 1450, 1702, 2023, 2211
Bsp143I GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
Bsp1720I GCTNAGC 1 cut(s) 1110
BspACI CCGC 5 cut(s) 601, 1114, 1232, 1932, 2130
BspANI GGCC 5 cut(s) 1198, 1773, 1863, 1895, 1908
BspCNI CTCAG 7 cut(s) 136, 1102, 1503, 1560, 1679, 1819, 2114
BspLI GGNNCC 5 cut(s) 562, 1413, 1783, 2090, 2128
BspMAI CTGCAG 1 cut(s) 1471
BspMI ACCTGC 1 cut(s) 562
BspOI GCTAGC 2 cut(s) 837, 1932
BspPI GGATC 7 cut(s) 211, 555, 568, 623, 1467, 1776, 1789
BspQI GCTCTTC 1 cut(s) 1671
BspT104I TTCGAA 2 cut(s) 179, 2242
BspTI CTTAAG 1 cut(s) 1244
BspTNI GGTCTC 1 cut(s) 337
BsrBI CCGCTC 1 cut(s) 1114
BsrDI GCAATG 3 cut(s) 165, 589, 1168
BsrGI TGTACA 5 cut(s) 293, 1450, 1702, 2023, 2211
BsrI ACTGG 4 cut(s) 274, 1252, 1978, 2094
BssECI CCNNGG 1 cut(s) 1119
BssMI GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
BssNAI GTATAC 1 cut(s) 954
BssNI GRCGYC 1 cut(s) 9
BssSI CACGAG 1 cut(s) 2249
BssT1I CCWWGG 1 cut(s) 1119
Bst1107I GTATAC 1 cut(s) 954
Bst2BI CACGAG 1 cut(s) 2249
Bst4CI ACNGT 4 cut(s) 278, 2011, 2021, 2028
Bst6I CTCTTC 4 cut(s) 439, 870, 1671, 1795
BstACI GRCGYC 1 cut(s) 9
BstAFI CTTAAG 1 cut(s) 1244
BstAPI GCANNNNNTGC 1 cut(s) 546
BstAUI TGTACA 5 cut(s) 293, 1450, 1702, 2023, 2211
BstBI TTCGAA 2 cut(s) 179, 2242
BstC8I GCNNGC 8 cut(s) 188, 835, 839, 1159, 1897, 1910, 1930, 2270
BstDEI CTNAG 8 cut(s) 123, 887, 1110, 1511, 1547, 1666, 1827, 2101
BstF5I GGATG 6 cut(s) 30, 744, 755, 1025, 1180, 2032
BstH2I RGCGCY 1 cut(s) 1252
BstHHI GCGC 2 cut(s) 1251, 2181
BstKTI GATC 8 cut(s) 206, 563, 631, 1462, 1568, 1607, 1665, 1784
BstMAI GTCTC 5 cut(s) 260, 337, 378, 511, 2195
BstMBI GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
BstMWI GCNNNNNNNGC 7 cut(s) 347, 546, 598, 1397, 1905, 2126, 2187
BstSFI CTRYAG 2 cut(s) 261, 1467
BstSLI GKGCMC 3 cut(s) 276, 542, 2290
BstV1I GCAGC 4 cut(s) 729, 1057, 1409, 1453
BstV2I GAAGAC 1 cut(s) 1040
BstX2I RGATCY 4 cut(s) 560, 1459, 1604, 1781
BstYI RGATCY 4 cut(s) 560, 1459, 1604, 1781
BstZ17I GTATAC 1 cut(s) 954
Bsu36I CCTNAGG 1 cut(s) 1827
BsuRI GGCC 5 cut(s) 1198, 1773, 1863, 1895, 1908
BtrI CACGTC 1 cut(s) 830
BtsCI GGATG 6 cut(s) 30, 744, 755, 1025, 1180, 2032
BtsIMutI CAGTG 7 cut(s) 283, 749, 1259, 1508, 1555, 2033, 2101
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 8 cut(s) 188, 835, 839, 1159, 1897, 1910, 1930, 2270
CfoI GCGC 2 cut(s) 1251, 2181
Cfr13I GGNCC 6 cut(s) 1116, 1196, 1598, 1772, 2088, 2135
Csp6I GTAC 5 cut(s) 294, 1451, 1703, 2024, 2212
CviAII CATG 6 cut(s) 524, 851, 929, 1211, 1682, 2164
CviQI GTAC 5 cut(s) 294, 1451, 1703, 2024, 2212
DdeI CTNAG 8 cut(s) 123, 887, 1110, 1511, 1547, 1666, 1827, 2101
DpnI GATC 8 cut(s) 205, 562, 630, 1461, 1567, 1606, 1664, 1783
DpnII GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
DraI TTTAAA 1 cut(s) 654
DraIII CACNNNGTG 1 cut(s) 1550
DriI GACNNNNNGTC 2 cut(s) 56, 759
EaeI YGGCCR 2 cut(s) 1861, 1906
Eam1104I CTCTTC 4 cut(s) 439, 870, 1671, 1795
Eam1105I GACNNNNNGTC 2 cut(s) 56, 759
EarI CTCTTC 4 cut(s) 439, 870, 1671, 1795
Ecl136II GAGCTC 1 cut(s) 1328
Eco130I CCWWGG 1 cut(s) 1119
Eco24I GRGCYC 2 cut(s) 1330, 1695
Eco31I GGTCTC 1 cut(s) 337
Eco32I GATATC 2 cut(s) 667, 1687
Eco47I GGWCC 4 cut(s) 1116, 1598, 2088, 2135
Eco53kI GAGCTC 1 cut(s) 1328
Eco57I CTGAAG 3 cut(s) 970, 1365, 2280
Eco81I CCTNAGG 1 cut(s) 1827
Eco88I CYCGRG 1 cut(s) 1524
EcoICRI GAGCTC 1 cut(s) 1328
EcoRI GAATTC 2 cut(s) 175, 1725
EcoRV GATATC 2 cut(s) 667, 1687
EcoT14I CCWWGG 1 cut(s) 1119
EcoT22I ATGCAT 2 cut(s) 715, 813
EcoT38I GRGCYC 2 cut(s) 1330, 1695
ErhI CCWWGG 1 cut(s) 1119
FaeI CATG 6 cut(s) 527, 854, 932, 1214, 1685, 2167
FalI AAGNNNNNCTT 2 cut(s) 1446, 1478
FaqI GGGAC 3 cut(s) 290, 1144, 2090
FatI CATG 6 cut(s) 523, 850, 928, 1210, 1681, 2163
FauNDI CATATG 1 cut(s) 1272
FblI GTMKAC 3 cut(s) 60, 953, 1978
Fnu4HI GCNGC 6 cut(s) 743, 1071, 1233, 1398, 1467, 2130
FokI GGATG 6 cut(s) 17, 742, 751, 1012, 1187, 2019
FriOI GRGCYC 2 cut(s) 1330, 1695
Fsp4HI GCNGC 6 cut(s) 743, 1071, 1233, 1398, 1467, 2130
FspBI CTAG 9 cut(s) 240, 678, 695, 834, 1074, 1640, 1749, 1929, 2058
GlaI GCGC 2 cut(s) 1250, 2180
GluI GCNGC 6 cut(s) 743, 1071, 1233, 1398, 1467, 2130
GsuI CTGGAG 4 cut(s) 250, 357, 363, 981
HaeII RGCGCY 1 cut(s) 1252
HaeIII GGCC 5 cut(s) 1198, 1773, 1863, 1895, 1908
HapII CCGG 1 cut(s) 2036
HhaI GCGC 2 cut(s) 1251, 2181
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 6 cut(s) 527, 854, 932, 1214, 1685, 2167
Hin6I GCGC 2 cut(s) 1249, 2179
HinP1I GCGC 2 cut(s) 1249, 2179
HincII GTYRAC 2 cut(s) 61, 793
HindII GTYRAC 2 cut(s) 61, 793
HindIII AAGCTT 4 cut(s) 248, 1347, 1711, 2266
HinfI GANTC 7 cut(s) 847, 1005, 1093, 1475, 1619, 1742, 2168
HpaI GTTAAC 1 cut(s) 793
HpaII CCGG 1 cut(s) 2036
HphI GGTGA 4 cut(s) 947, 1541, 1682, 1928
Hpy166II GTNNAC 8 cut(s) 61, 793, 827, 954, 1451, 1979, 2017, 2024
Hpy8I GTNNAC 8 cut(s) 61, 793, 827, 954, 1451, 1979, 2017, 2024
Hpy99I CGWCG 5 cut(s) 26, 62, 65, 106, 1663
HpyAV CCTTC 3 cut(s) 108, 729, 2255
HpyCH4III ACNGT 4 cut(s) 278, 2011, 2021, 2028
HpyCH4IV ACGT 6 cut(s) 57, 63, 823, 829, 1065, 1658
HpyF10VI GCNNNNNNNGC 7 cut(s) 347, 546, 598, 1397, 1905, 2126, 2187
HpyF3I CTNAG 8 cut(s) 123, 887, 1110, 1511, 1547, 1666, 1827, 2101
HpySE526I ACGT 6 cut(s) 57, 63, 823, 829, 1065, 1658
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 6 cut(s) 527, 854, 932, 1214, 1685, 2167
HspAI GCGC 2 cut(s) 1249, 2179
KspAI GTTAAC 1 cut(s) 793
Kzo9I GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
LguI GCTCTTC 1 cut(s) 1671
Lsp1109I GCAGC 4 cut(s) 729, 1057, 1409, 1453
LweI GCATC 9 cut(s) 64, 84, 154, 337, 700, 729, 820, 1024, 1034
MaeI CTAG 9 cut(s) 240, 678, 695, 834, 1074, 1640, 1749, 1929, 2058
MaeII ACGT 6 cut(s) 57, 63, 823, 829, 1065, 1658
MaeIII GTNAC 2 cut(s) 618, 1550
MalI GATC 8 cut(s) 205, 562, 630, 1461, 1567, 1606, 1664, 1783
MbiI CCGCTC 1 cut(s) 1114
MboI GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
MboII GAAGA 8 cut(s) 456, 515, 887, 1040, 1628, 1688, 1746, 1782
MflI RGATCY 4 cut(s) 560, 1459, 1604, 1781
MhlI GDGCHC 8 cut(s) 276, 542, 778, 906, 1330, 1510, 1695, 2290
MlsI TGGCCA 2 cut(s) 1863, 1908
MluNI TGGCCA 2 cut(s) 1863, 1908
MlyI GAGTC 2 cut(s) 841, 1014
MmeI TCCRAC 5 cut(s) 224, 451, 743, 1152, 1855
Mox20I TGGCCA 2 cut(s) 1863, 1908
Mph1103I ATGCAT 2 cut(s) 715, 813
MroXI GAANNNNTTC 4 cut(s) 118, 879, 1536, 1741
MscI TGGCCA 2 cut(s) 1863, 1908
MseI TTAA 9 cut(s) 159, 194, 366, 380, 653, 792, 1245, 1715, 2186
MslI CAYNNNNRTG 4 cut(s) 933, 1869, 2196, 2281
Msp20I TGGCCA 2 cut(s) 1863, 1908
MspA1I CMGCKG 2 cut(s) 422, 2132
MspCI CTTAAG 1 cut(s) 1244
MspI CCGG 1 cut(s) 2036
Mva1269I GAATGC 2 cut(s) 256, 2250
MwoI GCNNNNNNNGC 7 cut(s) 347, 546, 598, 1397, 1905, 2126, 2187
NdeI CATATG 1 cut(s) 1272
NdeII GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
NheI GCTAGC 2 cut(s) 833, 1928
NlaIII CATG 6 cut(s) 527, 854, 932, 1214, 1685, 2167
NlaIV GGNNCC 5 cut(s) 562, 1413, 1783, 2090, 2128
NmuCI GTSAC 2 cut(s) 618, 1550
NsiI ATGCAT 2 cut(s) 715, 813
NspV TTCGAA 2 cut(s) 179, 2242
PaeR7I CTCGAG 1 cut(s) 1524
PaqCI CACCTGC 1 cut(s) 562
PciSI GCTCTTC 1 cut(s) 1671
PcsI WCGNNNNNNNCGW 1 cut(s) 1444
PctI GAATGC 2 cut(s) 256, 2250
PdmI GAANNNNTTC 4 cut(s) 118, 879, 1536, 1741
PfeI GAWTC 5 cut(s) 1093, 1475, 1619, 1742, 2168
PflMI CCANNNNNTGG 4 cut(s) 171, 557, 1408, 1502
PkrI GCNGC 6 cut(s) 744, 1072, 1234, 1399, 1468, 2131
PleI GAGTC 2 cut(s) 841, 1013
PpsI GAGTC 2 cut(s) 841, 1013
PsiI TTATAA 1 cut(s) 1293
Psp124BI GAGCTC 1 cut(s) 1330
Psp1406I AACGTT 1 cut(s) 1065
PspN4I GGNNCC 5 cut(s) 562, 1413, 1783, 2090, 2128
PspPI GGNCC 6 cut(s) 1116, 1196, 1598, 1772, 2088, 2135
PspXI VCTCGAGB 1 cut(s) 1524
PstI CTGCAG 1 cut(s) 1471
PsuI RGATCY 4 cut(s) 560, 1459, 1604, 1781
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 5 cut(s) 295, 1452, 1704, 2025, 2213
RsaNI GTAC 5 cut(s) 294, 1451, 1703, 2024, 2212
RseI CAYNNNNRTG 4 cut(s) 933, 1869, 2196, 2281
SacI GAGCTC 1 cut(s) 1330
SalI GTCGAC 1 cut(s) 59
SapI GCTCTTC 1 cut(s) 1671
SaqAI TTAA 9 cut(s) 159, 194, 366, 380, 653, 792, 1245, 1715, 2186
SatI GCNGC 6 cut(s) 743, 1071, 1233, 1398, 1467, 2130
Sau3AI GATC 8 cut(s) 203, 560, 628, 1459, 1565, 1604, 1662, 1781
Sau96I GGNCC 6 cut(s) 1116, 1196, 1598, 1772, 2088, 2135
SchI GAGTC 2 cut(s) 841, 1014
SduI GDGCHC 8 cut(s) 276, 542, 778, 906, 1330, 1510, 1695, 2290
SfaNI GCATC 9 cut(s) 64, 84, 154, 337, 700, 729, 820, 1024, 1034
SfcI CTRYAG 2 cut(s) 261, 1467
Sfr274I CTCGAG 1 cut(s) 1524
SfuI TTCGAA 2 cut(s) 179, 2242
SgrDI CGTCGACG 1 cut(s) 59
SinI GGWCC 4 cut(s) 1116, 1598, 2088, 2135
SlaI CTCGAG 1 cut(s) 1524
SmiMI CAYNNNNRTG 4 cut(s) 933, 1869, 2196, 2281
SmlI CTYRAG 4 cut(s) 1244, 1425, 1524, 1720
SmoI CTYRAG 4 cut(s) 1244, 1425, 1524, 1720
SpeI ACTAGT 1 cut(s) 2057
SsiI CCGC 5 cut(s) 601, 1114, 1232, 1932, 2130
SspI AATATT 1 cut(s) 1699
SspMI CTAG 9 cut(s) 240, 678, 695, 834, 1074, 1640, 1749, 1929, 2058
SstI GAGCTC 1 cut(s) 1330
StyI CCWWGG 1 cut(s) 1119
TaaI ACNGT 4 cut(s) 278, 2011, 2021, 2028
TaiI ACGT 6 cut(s) 60, 66, 826, 832, 1068, 1661
TaqI TCGA 9 cut(s) 60, 179, 358, 1008, 1525, 1661, 1689, 1732, 2242
TatI WGTACW 5 cut(s) 293, 1450, 1702, 2023, 2211
TauI GCSGC 2 cut(s) 1235, 2132
TfiI GAWTC 5 cut(s) 1093, 1475, 1619, 1742, 2168
Tru1I TTAA 9 cut(s) 159, 194, 366, 380, 653, 792, 1245, 1715, 2186
Tru9I TTAA 9 cut(s) 159, 194, 366, 380, 653, 792, 1245, 1715, 2186
TscAI CASTG 7 cut(s) 283, 756, 1259, 1515, 1555, 2033, 2101
TseFI GTSAC 2 cut(s) 618, 1550
TseI GCWGC 4 cut(s) 742, 1070, 1397, 1466
Tsp45I GTSAC 2 cut(s) 618, 1550
TspDTI ATGAA 7 cut(s) 932, 1259, 1689, 1718, 1821, 1859, 2019
TspGWI ACGGA 4 cut(s) 232, 784, 885, 1895
TspRI CASTG 7 cut(s) 283, 756, 1259, 1515, 1555, 2033, 2101
Van91I CCANNNNNTGG 4 cut(s) 171, 557, 1408, 1502
Vha464I CTTAAG 1 cut(s) 1244
VpaK11BI GGWCC 4 cut(s) 1116, 1598, 2088, 2135
XapI RAATTY 8 cut(s) 175, 299, 643, 1385, 1420, 1725, 2082, 2226
XcmI CCANNNNNNNNNTGG 1 cut(s) 1871
XhoI CTCGAG 1 cut(s) 1524
XmiI GTMKAC 3 cut(s) 60, 953, 1978
XmnI GAANNNNTTC 4 cut(s) 118, 879, 1536, 1741
XspI CTAG 9 cut(s) 240, 678, 695, 834, 1074, 1640, 1749, 1929, 2058
Zsp2I ATGCAT 2 cut(s) 715, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.