pycom15g34530

Mechanosensitive ion channel protein

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
Physical Location & Seq
Reverse (-)
34320573 .. 34324289
3717 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1659 bp
ATGACTTCTTACCTTCGGCCCTTGCTTCTCTGGACTGGAGCAATACTTTTATGCAGAGCATTGGATCCTGTAGTCCTACCTTCAGAAGCAAGCCAGGCTGTCAAGCAACGTCTTATAAATTTTACACAATCGTTATCAACAGTGCTGGCATTTGCCTATTGTTTGTCAAGCTTAATTCAACGAGCACAAAAGTTCTTTACGGAGACAAGTGACACAAGAAATATGGGCTTCAGTTTCGCTGGAAAGGCAGTTTATTCTGCAGTTTGGGTTGCTGCGGTATCATTGTTCATGGAATTGCTGGGATTCTCAACCCAGAGATGGGTAACGGCTGGGGGTCTTGGCACAGTATTGCTCACCCTTGCTGGTCGTGAGATATTCACAAACTTCCTTTCGAGTGTAATGATTCATGCCACACGCCCATTTGTTGCTAATGAATGGATTCAGACTAAGATTCAAGGTTACGAAGTTTCTGGTACTGTTGAGCATGTGGGTTGGTGGTCCCCAACAATTATAAGGGGCGATGATCGTGAAGCAGTTCACATTCCAAATCACAAGTTCACTGTAACTGTTGTCCGGAATCTTAGCCAGAAAACTCATTGGCGAATCAAGACCCACCTCGCAGTCAGTCACTTGGATGTAAATAAAATTCATACTATAGTAGCTGATATGCGTAAGGTTCTGGCAAAAAATCCTCAAGTAGAGCAGCAAAGGTTGCATAGAAGAGTTTTTCTGGACGATATTAATCCGGAAAACCAGGCTCTTATGATTTTGGTCTCTTGCTTTGTTAAAACACCTCGTATTGAGGAGTATCTCTGCGTAAAGGAGGCAATACTTATGGATCTTCTTAGAGTTATTAGCCATCACCGTGCCCGGCTTGCCACACCCATCCGCACAGTCCAGAAATATTACAGTGAGGCCGACTTGGAAAATGTGCCATTCGCTGACACCGTTTTCACTCATTCATCTAGGGCATCCACTAATCGTCCGCATCTACTTATTGAACCATCTTACAAAATTAGTAGTGACGACAAGGGCAAGGCTTCAACTCACACAACCCGTACAAATGGAGAAAAACATTCTGAAGCGGCCTCAACATCTGACTCCAAGGGATCAAATACTAAAGCTGGGCCAGCAGTAACTCATGCACAAACTGACGACAAGGTTGTAGTGACTTTAAGTTCCAACTCAAGCACAACCTCTAAGGCTTCTGAAATGCCACCATCTGAACCCCAAACAAGGAATTCCGCTTCTGATGGTTCAGTTCGAAGTAACTCTGAGATGGTGCGGTCCAAGAACGAGAGTACAAAATATGCAGGGAAGGATACAACAGGAGTTAATTCCAAGGATGCATCTCCACAAAAGGTGACGGCTAAGAAGCCCCCTGATGCTTCACCCGAGACTGGTAGCGAAAAAGCAGATGTTCCTTTGACCACTACGCAGGCAAAGCAAGATGGTGAGAAACCTGTTTCAGCACCATCAGCAGCTAGGCCTCCGTTAGAAGAGAATATTATCCTTGGTGTTGCTTTGGAGGGCTCAAGGCGAACGCTTCCAATTGAGGAAGATACCACTCCATCTCCTACTGCAGGATCAAAAGAGTTCAGCGGCAGCCGTAATGGTAGCGGGTCTCCTTCCGGTGGCACGGATGTGAAGGATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families

Protein Analysis

553

Amino Acids

60.27

Weight (kDa)

9.1

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MSL2_3 PF25237 1 - 123 1.9e-65 Mechanosensitive channel protein 2/3 domain
MS_channel_2nd PF00924 125 - 195 2.1e-11 Mechanosensitive ion channel, beta-domain
Msl2-3_C PF24956 200 - 286 4.1e-44 Mechanosensitive ion channel protein 2/3 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 116, 512
AccIII TCCGGA 2 cut(s) 573, 745
AciI CCGC 8 cut(s) 275, 889, 986, 1085, 1245, 1285, 1602, 1620
AclWI GGATC 5 cut(s) 59, 72, 846, 1117, 1594
AcsI RAATTY 3 cut(s) 118, 645, 1240
AcuI CTGAAG 3 cut(s) 66, 214, 1101
AfaI GTAC 3 cut(s) 475, 1060, 1303
AfiI CCNNNNNNNGG 6 cut(s) 318, 319, 1236, 1400, 1583, 1634
AgsI TTSAA 4 cut(s) 179, 455, 1001, 1044
AjnI CCWGG 2 cut(s) 93, 753
AleI CACNNNNGTG 1 cut(s) 1643
AluBI AGCT 4 cut(s) 171, 662, 1124, 1484
AluI AGCT 4 cut(s) 171, 662, 1124, 1484
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 4 cut(s) 197, 778, 1391, 1629
AlwI GGATC 5 cut(s) 59, 72, 846, 1117, 1594
Ama87I CYCGRG 1 cut(s) 1394
Aor13HI TCCGGA 2 cut(s) 573, 745
AoxI GGCC 5 cut(s) 17, 915, 1086, 1127, 1487
ApeKI GCWGC 4 cut(s) 272, 703, 1481, 1605
ApoI RAATTY 3 cut(s) 118, 645, 1240
AseI ATTAAT 1 cut(s) 741
Asp700I GAANNNNTTC 2 cut(s) 438, 534
AspS9I GGNCC 4 cut(s) 18, 498, 1127, 1287
AsuC2I CCSGG 1 cut(s) 871
AsuHPI GGTGA 5 cut(s) 346, 854, 1375, 1383, 1466
AsuII TTCGAA 1 cut(s) 1264
AvaI CYCGRG 1 cut(s) 1394
AvaII GGWCC 2 cut(s) 498, 1287
BaeGI GKGCMC 1 cut(s) 871
BamHI GGATCC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 1535
Bbv12I GWGCWC 1 cut(s) 187
BbvI GCAGC 4 cut(s) 259, 715, 1493, 1617
BceAI ACGGC 3 cut(s) 342, 1383, 1593
BciT130I CCWGG 2 cut(s) 95, 755
BciVI GTATCC 1 cut(s) 1315
BcnI CCSGG 1 cut(s) 871
BcoDI GTCTC 4 cut(s) 197, 778, 1391, 1629
BfaI CTAG 2 cut(s) 966, 1485
BfmI CTRYAG 4 cut(s) 69, 258, 654, 1581
BfuI GTATCC 1 cut(s) 1315
BisI GCNGC 6 cut(s) 273, 704, 1086, 1482, 1603, 1606
BlsI GCNGC 6 cut(s) 274, 705, 1087, 1483, 1604, 1607
Bme1390I CCNGG 3 cut(s) 95, 755, 871
Bme18I GGWCC 2 cut(s) 498, 1287
BmeT110I CYCGRG 1 cut(s) 1394
BmgT120I GGNCC 4 cut(s) 18, 498, 1127, 1287
BmiI GGNNCC 2 cut(s) 66, 500
BmrFI CCNGG 3 cut(s) 95, 755, 871
BmsI GCATC 5 cut(s) 980, 997, 1336, 1358, 1375
BpmI CTGGAG 1 cut(s) 57
Bpu14I TTCGAA 1 cut(s) 1264
BpuEI CTTGAG 3 cut(s) 678, 1171, 1519
BpuMI CCSGG 1 cut(s) 871
BsaBI GATNNNNATC 1 cut(s) 741
BsaI GGTCTC 2 cut(s) 778, 1629
BsaJI CCNNGG 3 cut(s) 1104, 1341, 1513
BsaWI WCCGGW 3 cut(s) 573, 745, 1631
BsaXI ACNNNNNCTCC 2 cut(s) 1558, 1588
Bsc4I CCNNNNNNNGG 6 cut(s) 318, 319, 1236, 1400, 1583, 1634
Bse1I ACTGG 2 cut(s) 40, 1405
Bse8I GATNNNNATC 1 cut(s) 741
BseAI TCCGGA 2 cut(s) 573, 745
BseBI CCWGG 2 cut(s) 95, 755
BseDI CCNNGG 3 cut(s) 1104, 1341, 1513
BseGI GGATG 6 cut(s) 640, 885, 971, 1351, 1648, 1657
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 6 cut(s) 318, 319, 1236, 1400, 1583, 1634
BseMII CTCAG 1 cut(s) 1266
BseNI ACTGG 2 cut(s) 40, 1405
BseRI GAGGAG 1 cut(s) 818
BseSI GKGCMC 1 cut(s) 871
BseXI GCAGC 4 cut(s) 259, 715, 1493, 1617
BseYI CCCAGC 3 cut(s) 298, 329, 1124
BshFI GGCC 5 cut(s) 19, 917, 1088, 1129, 1489
BsiHKAI GWGCWC 1 cut(s) 187
BsiHKCI CYCGRG 1 cut(s) 1394
BsiSI CCGG 4 cut(s) 574, 746, 871, 1632
BslFI GGGAC 1 cut(s) 484
BslI CCNNNNNNNGG 6 cut(s) 318, 319, 1236, 1400, 1583, 1634
BsmAI GTCTC 4 cut(s) 197, 778, 1391, 1629
BsmFI GGGAC 1 cut(s) 484
BsnI GGCC 5 cut(s) 19, 917, 1088, 1129, 1489
Bso31I GGTCTC 2 cut(s) 778, 1629
BsoBI CYCGRG 1 cut(s) 1394
Bsp119I TTCGAA 1 cut(s) 1264
Bsp1286I GDGCHC 3 cut(s) 187, 871, 1535
Bsp13I TCCGGA 2 cut(s) 573, 745
Bsp143I GATC 5 cut(s) 64, 523, 838, 1109, 1586
BspACI CCGC 8 cut(s) 275, 889, 986, 1085, 1245, 1285, 1602, 1620
BspANI GGCC 5 cut(s) 19, 917, 1088, 1129, 1489
BspCNI CTCAG 1 cut(s) 1267
BspEI TCCGGA 2 cut(s) 573, 745
BspLI GGNNCC 2 cut(s) 66, 500
BspMAI CTGCAG 2 cut(s) 262, 1585
BspPI GGATC 5 cut(s) 59, 72, 846, 1117, 1594
BspT104I TTCGAA 1 cut(s) 1264
BspTNI GGTCTC 2 cut(s) 778, 1629
BsrI ACTGG 2 cut(s) 40, 1405
BssECI CCNNGG 3 cut(s) 1104, 1341, 1513
BssMI GATC 5 cut(s) 64, 523, 838, 1109, 1586
BssT1I CCWWGG 3 cut(s) 1104, 1341, 1513
Bst2UI CCWGG 2 cut(s) 95, 755
Bst4CI ACNGT 9 cut(s) 142, 346, 478, 562, 568, 866, 895, 911, 949
Bst6I CTCTTC 2 cut(s) 715, 1494
BstAPI GCANNNNNTGC 1 cut(s) 712
BstBI TTCGAA 1 cut(s) 1264
BstC8I GCNNGC 5 cut(s) 91, 147, 876, 1131, 1440
BstDEI CTNAG 6 cut(s) 447, 581, 845, 1200, 1275, 1371
BstENI CCTNNNNNAGG 1 cut(s) 1581
BstF5I GGATG 6 cut(s) 640, 885, 971, 1351, 1648, 1657
BstKTI GATC 5 cut(s) 67, 526, 841, 1112, 1589
BstMAI GTCTC 4 cut(s) 197, 778, 1391, 1629
BstMBI GATC 5 cut(s) 64, 523, 838, 1109, 1586
BstMWI GCNNNNNNNGC 7 cut(s) 95, 245, 712, 875, 1130, 1444, 1478
BstNI CCWGG 2 cut(s) 95, 755
BstNSI RCATGY 1 cut(s) 488
BstSCI CCNGG 3 cut(s) 93, 753, 869
BstSFI CTRYAG 4 cut(s) 69, 258, 654, 1581
BstSLI GKGCMC 1 cut(s) 871
BstV1I GCAGC 4 cut(s) 259, 715, 1493, 1617
BstX2I RGATCY 2 cut(s) 64, 838
BstYI RGATCY 2 cut(s) 64, 838
BsuI GTATCC 1 cut(s) 1315
BsuRI GGCC 5 cut(s) 19, 917, 1088, 1129, 1489
BtgZI GCGATG 1 cut(s) 534
BtsCI GGATG 6 cut(s) 640, 885, 971, 1351, 1648, 1657
BtsIMutI CAGTG 3 cut(s) 147, 558, 916
Cac8I GCNNGC 5 cut(s) 91, 147, 876, 1131, 1440
Cfr13I GGNCC 4 cut(s) 18, 498, 1127, 1287
Csp6I GTAC 3 cut(s) 474, 1059, 1302
CviAII CATG 4 cut(s) 289, 407, 485, 1142
CviQI GTAC 3 cut(s) 474, 1059, 1302
DdeI CTNAG 6 cut(s) 447, 581, 845, 1200, 1275, 1371
DpnI GATC 5 cut(s) 66, 525, 840, 1111, 1588
DpnII GATC 5 cut(s) 64, 523, 838, 1109, 1586
Eam1104I CTCTTC 2 cut(s) 715, 1494
EarI CTCTTC 2 cut(s) 715, 1494
Eco130I CCWWGG 3 cut(s) 1104, 1341, 1513
Eco147I AGGCCT 1 cut(s) 1489
Eco24I GRGCYC 1 cut(s) 1535
Eco31I GGTCTC 2 cut(s) 778, 1629
Eco47I GGWCC 2 cut(s) 498, 1287
Eco57I CTGAAG 3 cut(s) 66, 214, 1101
Eco88I CYCGRG 1 cut(s) 1394
EcoNI CCTNNNNNAGG 1 cut(s) 1581
EcoRI GAATTC 1 cut(s) 1240
EcoRII CCWGG 2 cut(s) 93, 753
EcoT14I CCWWGG 3 cut(s) 1104, 1341, 1513
EcoT22I ATGCAT 1 cut(s) 1351
EcoT38I GRGCYC 1 cut(s) 1535
ErhI CCWWGG 3 cut(s) 1104, 1341, 1513
FaeI CATG 4 cut(s) 292, 410, 488, 1145
FaqI GGGAC 1 cut(s) 484
FatI CATG 4 cut(s) 288, 406, 484, 1141
FauI CCCGC 1 cut(s) 1613
Fnu4HI GCNGC 6 cut(s) 273, 704, 1086, 1482, 1603, 1606
FokI GGATG 5 cut(s) 647, 872, 958, 1358, 1655
FriOI GRGCYC 1 cut(s) 1535
Fsp4HI GCNGC 6 cut(s) 273, 704, 1086, 1482, 1603, 1606
FspBI CTAG 2 cut(s) 966, 1485
GluI GCNGC 6 cut(s) 273, 704, 1086, 1482, 1603, 1606
GsaI CCCAGC 3 cut(s) 302, 333, 1128
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 5 cut(s) 19, 917, 1088, 1129, 1489
HapII CCGG 4 cut(s) 574, 746, 871, 1632
Hin1II CATG 4 cut(s) 292, 410, 488, 1145
HindIII AAGCTT 1 cut(s) 169
HinfI GANTC 7 cut(s) 303, 403, 439, 451, 577, 603, 1100
HpaII CCGG 4 cut(s) 574, 746, 871, 1632
HphI GGTGA 5 cut(s) 346, 854, 1375, 1383, 1466
Hpy166II GTNNAC 2 cut(s) 538, 558
Hpy188I TCNGA 8 cut(s) 85, 444, 1081, 1099, 1210, 1225, 1252, 1276
Hpy188III TCNNGA 8 cut(s) 31, 368, 527, 574, 607, 731, 746, 898
Hpy8I GTNNAC 2 cut(s) 538, 558
HpyAV CCTTC 5 cut(s) 23, 90, 1312, 1638, 1642
HpyCH4III ACNGT 9 cut(s) 142, 346, 478, 562, 568, 866, 895, 911, 949
HpyCH4IV ACGT 1 cut(s) 109
HpyCH4V TGCA 7 cut(s) 54, 260, 715, 1145, 1313, 1349, 1583
HpyF10VI GCNNNNNNNGC 7 cut(s) 95, 245, 712, 875, 1130, 1444, 1478
HpyF3I CTNAG 6 cut(s) 447, 581, 845, 1200, 1275, 1371
HpySE526I ACGT 1 cut(s) 109
Hsp92II CATG 4 cut(s) 292, 410, 488, 1145
Kpn2I TCCGGA 2 cut(s) 573, 745
Kzo9I GATC 5 cut(s) 64, 523, 838, 1109, 1586
LmnI GCTCC 1 cut(s) 38
Lsp1109I GCAGC 4 cut(s) 259, 715, 1493, 1617
LweI GCATC 5 cut(s) 980, 997, 1336, 1358, 1375
MaeI CTAG 2 cut(s) 966, 1485
MaeII ACGT 1 cut(s) 109
MalI GATC 5 cut(s) 66, 525, 840, 1111, 1588
MboI GATC 5 cut(s) 64, 523, 838, 1109, 1586
MboII GAAGA 4 cut(s) 732, 833, 1511, 1571
MfeI CAATTG 1 cut(s) 1551
MflI RGATCY 2 cut(s) 64, 838
MhlI GDGCHC 3 cut(s) 187, 871, 1535
MluCI AATT 9 cut(s) 118, 174, 293, 507, 645, 1014, 1240, 1336, 1551
MlyI GAGTC 1 cut(s) 1094
MmeI TCCRAC 1 cut(s) 1206
Mph1103I ATGCAT 1 cut(s) 1351
MroI TCCGGA 2 cut(s) 573, 745
MroXI GAANNNNTTC 2 cut(s) 438, 534
MseI TTAA 5 cut(s) 173, 741, 786, 1175, 1335
MslI CAYNNNNRTG 3 cut(s) 633, 864, 1643
MspA1I CMGCKG 1 cut(s) 1602
MspI CCGG 4 cut(s) 574, 746, 871, 1632
MspR9I CCNGG 3 cut(s) 95, 755, 871
MunI CAATTG 1 cut(s) 1551
MvaI CCWGG 2 cut(s) 95, 755
MwoI GCNNNNNNNGC 7 cut(s) 95, 245, 712, 875, 1130, 1444, 1478
NciI CCSGG 1 cut(s) 871
NdeII GATC 5 cut(s) 64, 523, 838, 1109, 1586
NlaIII CATG 4 cut(s) 292, 410, 488, 1145
NlaIV GGNNCC 2 cut(s) 66, 500
NmuCI GTSAC 5 cut(s) 209, 626, 1022, 1168, 1363
NsiI ATGCAT 1 cut(s) 1351
NspI RCATGY 1 cut(s) 488
NspV TTCGAA 1 cut(s) 1264
OliI CACNNNNGTG 1 cut(s) 1643
PceI AGGCCT 1 cut(s) 1489
PcsI WCGNNNNNNNCGW 1 cut(s) 945
PdmI GAANNNNTTC 2 cut(s) 438, 534
PfeI GAWTC 6 cut(s) 303, 403, 439, 451, 577, 603
PkrI GCNGC 6 cut(s) 274, 705, 1087, 1483, 1604, 1607
PleI GAGTC 1 cut(s) 1094
PpsI GAGTC 1 cut(s) 1094
PshBI ATTAAT 1 cut(s) 741
PsiI TTATAA 2 cut(s) 116, 512
Psp6I CCWGG 2 cut(s) 93, 753
PspFI CCCAGC 3 cut(s) 298, 329, 1124
PspGI CCWGG 2 cut(s) 93, 753
PspN4I GGNNCC 2 cut(s) 66, 500
PspPI GGNCC 4 cut(s) 18, 498, 1127, 1287
PstI CTGCAG 2 cut(s) 262, 1585
PsuI RGATCY 2 cut(s) 64, 838
RsaI GTAC 3 cut(s) 475, 1060, 1303
RsaNI GTAC 3 cut(s) 474, 1059, 1302
RseI CAYNNNNRTG 3 cut(s) 633, 864, 1643
SaqAI TTAA 5 cut(s) 173, 741, 786, 1175, 1335
SatI GCNGC 6 cut(s) 273, 704, 1086, 1482, 1603, 1606
Sau3AI GATC 5 cut(s) 64, 523, 838, 1109, 1586
Sau96I GGNCC 4 cut(s) 18, 498, 1127, 1287
SchI GAGTC 1 cut(s) 1094
ScrFI CCNGG 3 cut(s) 95, 755, 871
SduI GDGCHC 3 cut(s) 187, 871, 1535
SfaNI GCATC 5 cut(s) 980, 997, 1336, 1358, 1375
SfcI CTRYAG 4 cut(s) 69, 258, 654, 1581
SfuI TTCGAA 1 cut(s) 1264
SinI GGWCC 2 cut(s) 498, 1287
SmiMI CAYNNNNRTG 3 cut(s) 633, 864, 1643
SmlI CTYRAG 3 cut(s) 693, 1186, 1534
SmoI CTYRAG 3 cut(s) 693, 1186, 1534
Sse9I AATT 9 cut(s) 118, 174, 293, 507, 645, 1014, 1240, 1336, 1551
SseBI AGGCCT 1 cut(s) 1489
SsiI CCGC 8 cut(s) 275, 889, 986, 1085, 1245, 1285, 1602, 1620
SspI AATATT 2 cut(s) 905, 1507
SspMI CTAG 2 cut(s) 966, 1485
StuI AGGCCT 1 cut(s) 1489
StyD4I CCNGG 3 cut(s) 93, 753, 869
StyI CCWWGG 3 cut(s) 1104, 1341, 1513
TaaI ACNGT 9 cut(s) 142, 346, 478, 562, 568, 866, 895, 911, 949
TaiI ACGT 1 cut(s) 112
TaqI TCGA 2 cut(s) 392, 1264
TasI AATT 9 cut(s) 118, 174, 293, 507, 645, 1014, 1240, 1336, 1551
TatI WGTACW 1 cut(s) 1301
TauI GCSGC 2 cut(s) 1088, 1605
TfiI GAWTC 6 cut(s) 303, 403, 439, 451, 577, 603
Tru1I TTAA 5 cut(s) 173, 741, 786, 1175, 1335
Tru9I TTAA 5 cut(s) 173, 741, 786, 1175, 1335
TscAI CASTG 3 cut(s) 147, 565, 916
TseFI GTSAC 5 cut(s) 209, 626, 1022, 1168, 1363
TseI GCWGC 4 cut(s) 272, 703, 1481, 1605
Tsp45I GTSAC 5 cut(s) 209, 626, 1022, 1168, 1363
TspDTI ATGAA 5 cut(s) 277, 395, 447, 638, 951
TspGWI ACGGA 3 cut(s) 215, 1482, 1655
TspRI CASTG 3 cut(s) 147, 565, 916
VpaK11BI GGWCC 2 cut(s) 498, 1287
VspI ATTAAT 1 cut(s) 741
XagI CCTNNNNNAGG 1 cut(s) 1581
XapI RAATTY 3 cut(s) 118, 645, 1240
XceI RCATGY 1 cut(s) 488
XmnI GAANNNNTTC 2 cut(s) 438, 534
XspI CTAG 2 cut(s) 966, 1485
Zsp2I ATGCAT 1 cut(s) 1351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.