pycom15g35890

WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008538
Physical Location & Seq
Reverse (-)
35955308 .. 35964921
9614 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1542 bp
ATGTCTACCTTAGAAATCGAAGCTCGAGATGTCATCAAGATTGTGCTTCAATTTTGCAAAGAGAATTCTTTGCACCAAACGTTTCAAACGTTGCAGAGTGAGTGCCAGGTATCTTTGAACACTGTTGACAGCGTTGAGACATTTGTTTCTGACATAAATAGTGGAAGGTGGGATGCAATTTTGCCTCAAGTGGCTCAACTTAAGCTTCCCAGGAATAAATTGGAGGATTTGTACGAGCAGATTGTACTCGAAATGATTGAACTTCGAGAATTGGATACTGCTCGTGCTATCTTAAGACAGACTCAGGTAATGGGTGTAGTGAAGCAAGAACAACCTGAAAGATTCTTGCGACTTGAGCATCTCCTTGTCAGAACCTATTTTGATCCAAATGAGGCTTATCAAGAGTCAACAAAAGAAAAACGGCGTGCTCAAATTGCTCAAGCACTTGCTGCAGAAGTTAGTGTGGTGCCACCATCAAGATTAACAGCTCTAATTGGTCAGGCTCTGAAGTGGCAGCAGCACCAAGGATTGCTTCCCCCAGGAACACAGTTTGACTTATTTCGGGGAACAGCTGCTATGAAACAAGATGTGGATGACTTGTATCCAACAACTCTTTCACACACAATAAAGTTTGGGACAAAAAGTCATGCAGAATGTGCCCGGTTCTCACCAGATGGGCAGTTTCTTGTCTCTTGCTCGGTTGATGGATTTATTGAGGTGTGGGATTACTTAAGCGGGAAACTCAAGAAGGATCTTCAGTACCAGGCTGATGAAAGTTTCATGATGCACGAGGATGCTGTCCTCTGTGTTGAATTTAGCCGAGATTCAGAGATGCTTGCATCTGGGTCACAAGATGGAAAGATCAAAGTTTGGCGTATTAGGACTGGGCAGTGCTTGCGCCGTCTTGAACGTGCACATTCAAAGGGTGTCACCAGTGTTTCCTTCTCTCGTGATGGAAGCCAGTTACTTAGTACATCATTTGACAGCACTGCAAAAATCCATGGCCTAAAATCTGGGAAGGCGTTGAAAGAATTTCGTGGACATTCATCTTATGTAAATGATGCAATCTTTACAAGTGATGGAGCTCGTGTAATTACTGCATCTAGCGATTGTACGGTCAAGGTCTGGGATTTGAAGACTACAGACTGTCTGCAGACATTCAAGCCACCTCCTCCTTTAAGGGGAGGTGATGCTTCTGTGAATTCTGTTCATCTTTACCCAAAAAATACAGATCACATCATTGTATGTAATAAGACGTCATCCATTTATGTCATGACACTTCAAGGGCAGGTTGTGAAGAGCTTTTCATCTGGTAAAAAGGAAGGTGGAGATTTTTTGTCAGCTTGTGTGTCACCTAAAGGGGAATGGATTTACTGTGTTGGTGAAGACAGGAATATGTACTGTTTCAGTCACCAATCTGGTAAACTAGAGCATCTAATGAAGGTCCATGAAAAGGAGGTAATTGGCATCAGTCACCATCCTCACAGGAATCTTGTCGCCACATATAGCGAAGACTGCACACTGAAGTTATGGAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

514

Amino Acids

57.73

Weight (kDa)

6.79

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_TPL PF17814 7 - 37 3.2e-15 LisH-like dimerisation domain
WD40_Gbeta PF25391 197 - 387 7.6e-16 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 204 - 332 4.6e-13 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 208 - 328 1.2e-08 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 213 - 298 3.6e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 213 - 322 3.6e-16 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 214 - 512 3.1e-57 WDR5 beta-propeller domain
WDR55 PF24796 216 - 302 1.5e-08 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 216 - 390 5.6e-23 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 217 - 242 4.8e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 218 - 302 5.6e-15 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 221 - 376 2.1e-19 CDC20/Fizzy WD40 domain
EIF3I PF24805 221 - 303 1.4e-07 EIF3I
WDR90_beta-prop_4th PF23342 222 - 348 3.7e-07 WDR90, 4th beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 229 - 386 1.2e-14 CAF1B/HIR1 beta-propeller domain
Beta-prop_VPS8 PF23410 257 - 332 3.9e-07 Vps8 beta-propeller
Beta-prop_TEP1_2nd PF25047 257 - 382 1.4e-16 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 260 - 329 1.6e-07 WDR36/Utp21 first beta-propeller
Beta-prop_EML PF23409 260 - 359 8.1e-08 Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st PF24817 260 - 303 5.5e-07 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 260 - 387 1e-29 WDR3 first beta-propeller domain
WD40 PF00400 262 - 291 9.7e-08 WD domain, G-beta repeat
Beta-prop_Aladin PF25460 265 - 383 1.2e-07 Aladin seven-bladed propeller
Beta-prop_ACSF4 PF13570 273 - 484 3e-06 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 287 - 367 5.5e-06 WDR90/POC16, second beta-propeller
WD40 PF00400 296 - 333 1.5e-06 WD domain, G-beta repeat
WDR55 PF24796 304 - 388 3.5e-10 WDR55
WD40_Prp19 PF24814 304 - 452 1.4e-14 Prp19 WD40 domain
EIF3I PF24805 305 - 511 1.9e-17 EIF3I
Beta-prop_THOC3 PF25174 306 - 511 5.9e-28 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 309 - 500 1.3e-16 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 310 - 471 1.7e-10 WDR3 second beta-propeller domain
WD40 PF00400 340 - 377 1.1e-08 WD domain, G-beta repeat
WD40_Prp19 PF24814 454 - 512 2.9e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1259
Acc36I ACCTGC 1 cut(s) 1279
AccB1I GGYRCC 1 cut(s) 466
AccI GTMKAC 1 cut(s) 5
AciI CCGC 1 cut(s) 735
AclI AACGTT 2 cut(s) 80, 89
AclWI GGATC 2 cut(s) 377, 759
AcsI RAATTY 4 cut(s) 64, 812, 1031, 1201
AcuI CTGAAG 2 cut(s) 527, 740
AcyI GRCGYC 1 cut(s) 1256
AfaI GTAC 6 cut(s) 233, 246, 761, 973, 1114, 1400
AfiI CCNNNNNNNGG 2 cut(s) 1181, 1453
AflII CTTAAG 3 cut(s) 200, 292, 730
AhdI GACNNNNNGTC 1 cut(s) 642
AjnI CCWGG 4 cut(s) 105, 209, 538, 762
AjuI GAANNNNNNNTTGG 4 cut(s) 516, 548, 598, 630
AluBI AGCT 7 cut(s) 23, 205, 488, 572, 1085, 1302, 1343
AluI AGCT 7 cut(s) 23, 205, 488, 572, 1085, 1302, 1343
Alw21I GWGCWC 3 cut(s) 430, 916, 1087
Alw26I GTCTC 2 cut(s) 131, 694
Alw44I GTGCAC 1 cut(s) 912
AlwI GGATC 2 cut(s) 377, 759
AlwNI CAGNNNCTG 1 cut(s) 505
Ama87I CYCGRG 1 cut(s) 24
AoxI GGCC 1 cut(s) 1003
ApaLI GTGCAC 1 cut(s) 912
ApeKI GCWGC 4 cut(s) 449, 514, 517, 572
ApoI RAATTY 4 cut(s) 64, 812, 1031, 1201
AspLEI GCGC 1 cut(s) 900
AspS9I GGNCC 1 cut(s) 1444
AsuC2I CCSGG 1 cut(s) 661
AsuHPI GGTGA 7 cut(s) 660, 922, 1199, 1344, 1394, 1403, 1466
AvaI CYCGRG 1 cut(s) 24
AvaII GGWCC 1 cut(s) 1444
BaeGI GKGCMC 2 cut(s) 661, 916
BanI GGYRCC 1 cut(s) 466
BanII GRGCYC 1 cut(s) 1087
BauI CACGAG 4 cut(s) 282, 788, 948, 1086
BbsI GAAGAC 3 cut(s) 1142, 1392, 1518
Bbv12I GWGCWC 3 cut(s) 430, 916, 1087
BbvI GCAGC 4 cut(s) 436, 526, 529, 559
BccI CCATC 7 cut(s) 481, 668, 698, 848, 947, 1073, 1485
BceAI ACGGC 2 cut(s) 437, 885
BciT130I CCWGG 4 cut(s) 107, 211, 540, 764
BciVI GTATCC 2 cut(s) 268, 612
BcnI CCSGG 1 cut(s) 661
BcoDI GTCTC 2 cut(s) 131, 694
BfaI CTAG 2 cut(s) 1104, 1427
BfmI CTRYAG 3 cut(s) 450, 1140, 1151
BfrI CTTAAG 3 cut(s) 200, 292, 730
BfuAI ACCTGC 1 cut(s) 1279
BfuI GTATCC 2 cut(s) 268, 612
BisI GCNGC 4 cut(s) 450, 515, 518, 573
BlsI GCNGC 4 cut(s) 451, 516, 519, 574
Bme1390I CCNGG 5 cut(s) 107, 211, 540, 661, 764
Bme18I GGWCC 1 cut(s) 1444
BmeRI GACNNNNNGTC 1 cut(s) 642
BmeT110I CYCGRG 1 cut(s) 24
BmgT120I GGNCC 1 cut(s) 1444
BmiI GGNNCC 1 cut(s) 468
BmrFI CCNGG 5 cut(s) 107, 211, 540, 661, 764
BmrI ACTGGG 1 cut(s) 894
BmuI ACTGGG 1 cut(s) 894
BpiI GAAGAC 3 cut(s) 1142, 1392, 1518
BpuEI CTTGAG 4 cut(s) 171, 374, 423, 728
BpuMI CCSGG 1 cut(s) 661
BsaHI GRCGYC 1 cut(s) 1256
BsaJI CCNNGG 4 cut(s) 209, 523, 538, 1000
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 1074, 1104
Bsc4I CCNNNNNNNGG 2 cut(s) 1181, 1453
Bse1I ACTGG 3 cut(s) 889, 933, 961
BseBI CCWGG 4 cut(s) 107, 211, 540, 764
BseDI CCNNGG 4 cut(s) 209, 523, 538, 1000
BseGI GGATG 5 cut(s) 178, 598, 799, 1259, 1477
BseLI CCNNNNNNNGG 2 cut(s) 1181, 1453
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 3 cut(s) 889, 933, 961
BseRI GAGGAG 1 cut(s) 1161
BseSI GKGCMC 2 cut(s) 661, 916
BseXI GCAGC 4 cut(s) 436, 526, 529, 559
BsgI GTGCAG 1 cut(s) 1501
BshFI GGCC 1 cut(s) 1005
BshNI GGYRCC 1 cut(s) 466
BsiHKAI GWGCWC 3 cut(s) 430, 916, 1087
BsiHKCI CYCGRG 1 cut(s) 24
BsiSI CCGG 1 cut(s) 661
BslFI GGGAC 1 cut(s) 649
BslI CCNNNNNNNGG 2 cut(s) 1181, 1453
BsmAI GTCTC 2 cut(s) 131, 694
BsmFI GGGAC 1 cut(s) 649
BsnI GGCC 1 cut(s) 1005
BsoBI CYCGRG 1 cut(s) 24
Bsp1286I GDGCHC 4 cut(s) 430, 661, 916, 1087
Bsp143I GATC 4 cut(s) 382, 751, 861, 1231
Bsp19I CCATGG 1 cut(s) 1000
BspACI CCGC 1 cut(s) 735
BspANI GGCC 1 cut(s) 1005
BspCNI CTCAG 1 cut(s) 316
BspHI TCATGA 2 cut(s) 780, 1272
BspLI GGNNCC 1 cut(s) 468
BspMAI CTGCAG 2 cut(s) 454, 1155
BspMI ACCTGC 1 cut(s) 1279
BspPI GGATC 2 cut(s) 377, 759
BspQI GCTCTTC 1 cut(s) 1292
BspT107I GGYRCC 1 cut(s) 466
BspTI CTTAAG 3 cut(s) 200, 292, 730
BsrI ACTGG 3 cut(s) 889, 933, 961
BssECI CCNNGG 4 cut(s) 209, 523, 538, 1000
BssMI GATC 4 cut(s) 382, 751, 861, 1231
BssNI GRCGYC 1 cut(s) 1256
BssSI CACGAG 4 cut(s) 282, 788, 948, 1086
BssT1I CCWWGG 2 cut(s) 523, 1000
Bst2BI CACGAG 4 cut(s) 282, 788, 948, 1086
Bst2UI CCWGG 4 cut(s) 107, 211, 540, 764
Bst4CI ACNGT 6 cut(s) 124, 549, 1117, 1148, 1376, 1403
Bst6I CTCTTC 1 cut(s) 1292
BstACI GRCGYC 1 cut(s) 1256
BstAFI CTTAAG 3 cut(s) 200, 292, 730
BstAPI GCANNNNNTGC 3 cut(s) 449, 656, 895
BstC8I GCNNGC 3 cut(s) 426, 837, 896
BstDEI CTNAG 3 cut(s) 10, 303, 968
BstDSI CCRYGG 1 cut(s) 1000
BstF5I GGATG 5 cut(s) 178, 598, 799, 1259, 1477
BstHHI GCGC 1 cut(s) 900
BstKTI GATC 4 cut(s) 385, 754, 864, 1234
BstMAI GTCTC 2 cut(s) 131, 694
BstMBI GATC 4 cut(s) 382, 751, 861, 1231
BstMWI GCNNNNNNNGC 6 cut(s) 355, 434, 449, 656, 895, 1515
BstNI CCWGG 4 cut(s) 107, 211, 540, 764
BstSCI CCNGG 5 cut(s) 105, 209, 538, 659, 762
BstSFI CTRYAG 3 cut(s) 450, 1140, 1151
BstSLI GKGCMC 2 cut(s) 661, 916
BstV1I GCAGC 4 cut(s) 436, 526, 529, 559
BstV2I GAAGAC 3 cut(s) 1142, 1392, 1518
BstX2I RGATCY 1 cut(s) 751
BstYI RGATCY 1 cut(s) 751
BsuI GTATCC 2 cut(s) 268, 612
BsuRI GGCC 1 cut(s) 1005
BtgI CCRYGG 1 cut(s) 1000
BtsCI GGATG 5 cut(s) 178, 598, 799, 1259, 1477
BtsI GCAGTG 2 cut(s) 896, 987
BtsIMutI CAGTG 5 cut(s) 120, 896, 940, 987, 1520
BveI ACCTGC 1 cut(s) 1279
Cac8I GCNNGC 3 cut(s) 426, 837, 896
CaiI CAGNNNCTG 1 cut(s) 505
CciI TCATGA 2 cut(s) 780, 1272
CfoI GCGC 1 cut(s) 900
Cfr13I GGNCC 1 cut(s) 1444
Csp6I GTAC 6 cut(s) 232, 245, 760, 972, 1113, 1399
CviAII CATG 5 cut(s) 647, 781, 1001, 1273, 1448
CviQI GTAC 6 cut(s) 232, 245, 760, 972, 1113, 1399
DdeI CTNAG 3 cut(s) 10, 303, 968
DpnI GATC 4 cut(s) 384, 753, 863, 1233
DpnII GATC 4 cut(s) 382, 751, 861, 1231
DriI GACNNNNNGTC 1 cut(s) 642
Eam1104I CTCTTC 1 cut(s) 1292
Eam1105I GACNNNNNGTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 1292
Ecl136II GAGCTC 1 cut(s) 1085
Eco130I CCWWGG 2 cut(s) 523, 1000
Eco24I GRGCYC 1 cut(s) 1087
Eco47I GGWCC 1 cut(s) 1444
Eco53kI GAGCTC 1 cut(s) 1085
Eco57I CTGAAG 2 cut(s) 527, 740
Eco88I CYCGRG 1 cut(s) 24
EcoICRI GAGCTC 1 cut(s) 1085
EcoRI GAATTC 2 cut(s) 64, 1201
EcoRII CCWGG 4 cut(s) 105, 209, 538, 762
EcoT14I CCWWGG 2 cut(s) 523, 1000
EcoT38I GRGCYC 1 cut(s) 1087
ErhI CCWWGG 2 cut(s) 523, 1000
FaeI CATG 5 cut(s) 650, 784, 1004, 1276, 1451
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FaqI GGGAC 1 cut(s) 649
FatI CATG 5 cut(s) 646, 780, 1000, 1272, 1447
FauI CCCGC 1 cut(s) 728
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 450, 515, 518, 573
FokI GGATG 5 cut(s) 185, 605, 806, 1246, 1464
FriOI GRGCYC 1 cut(s) 1087
Fsp4HI GCNGC 4 cut(s) 450, 515, 518, 573
FspBI CTAG 2 cut(s) 1104, 1427
GlaI GCGC 1 cut(s) 899
GluI GCNGC 4 cut(s) 450, 515, 518, 573
HaeIII GGCC 1 cut(s) 1005
HapII CCGG 1 cut(s) 661
HhaI GCGC 1 cut(s) 900
Hin1I GRCGYC 1 cut(s) 1256
Hin1II CATG 5 cut(s) 650, 784, 1004, 1276, 1451
Hin6I GCGC 1 cut(s) 898
HinP1I GCGC 1 cut(s) 898
HincII GTYRAC 2 cut(s) 127, 408
HindII GTYRAC 2 cut(s) 127, 408
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 5 cut(s) 301, 342, 404, 824, 1489
HpaII CCGG 1 cut(s) 661
HphI GGTGA 7 cut(s) 660, 922, 1199, 1344, 1394, 1403, 1466
Hpy166II GTNNAC 6 cut(s) 6, 127, 408, 914, 1040, 1424
Hpy188I TCNGA 4 cut(s) 151, 371, 507, 829
Hpy8I GTNNAC 6 cut(s) 6, 127, 408, 914, 1040, 1424
HpyAV CCTTC 6 cut(s) 159, 742, 952, 1012, 1316, 1435
HpyCH4III ACNGT 6 cut(s) 124, 549, 1117, 1148, 1376, 1403
HpyCH4IV ACGT 4 cut(s) 80, 89, 910, 1256
HpyF10VI GCNNNNNNNGC 6 cut(s) 355, 434, 449, 656, 895, 1515
HpyF3I CTNAG 3 cut(s) 10, 303, 968
HpySE526I ACGT 4 cut(s) 80, 89, 910, 1256
Hsp92I GRCGYC 1 cut(s) 1256
Hsp92II CATG 5 cut(s) 650, 784, 1004, 1276, 1451
HspAI GCGC 1 cut(s) 898
Kzo9I GATC 4 cut(s) 382, 751, 861, 1231
LguI GCTCTTC 1 cut(s) 1292
LmnI GCTCC 1 cut(s) 1082
Lsp1109I GCAGC 4 cut(s) 436, 526, 529, 559
MaeI CTAG 2 cut(s) 1104, 1427
MaeII ACGT 4 cut(s) 80, 89, 910, 1256
MaeIII GTNAC 6 cut(s) 846, 928, 963, 1350, 1409, 1472
MalI GATC 4 cut(s) 384, 753, 863, 1233
MboI GATC 4 cut(s) 382, 751, 861, 1231
MboII GAAGA 5 cut(s) 746, 1147, 1309, 1397, 1523
MflI RGATCY 1 cut(s) 751
MhlI GDGCHC 4 cut(s) 430, 661, 916, 1087
MlyI GAGTC 2 cut(s) 295, 413
MmeI TCCRAC 1 cut(s) 629
MseI TTAA 5 cut(s) 201, 293, 482, 731, 1178
MslI CAYNNNNRTG 1 cut(s) 792
MspA1I CMGCKG 1 cut(s) 572
MspCI CTTAAG 3 cut(s) 200, 292, 730
MspI CCGG 1 cut(s) 661
MspR9I CCNGG 5 cut(s) 107, 211, 540, 661, 764
MvaI CCWGG 4 cut(s) 107, 211, 540, 764
MwoI GCNNNNNNNGC 6 cut(s) 355, 434, 449, 656, 895, 1515
NciI CCSGG 1 cut(s) 661
NcoI CCATGG 1 cut(s) 1000
NdeII GATC 4 cut(s) 382, 751, 861, 1231
NlaIII CATG 5 cut(s) 650, 784, 1004, 1276, 1451
NlaIV GGNNCC 1 cut(s) 468
NmeAIII GCCGAG 1 cut(s) 845
NmuCI GTSAC 5 cut(s) 846, 928, 1350, 1409, 1472
PaeR7I CTCGAG 1 cut(s) 24
PagI TCATGA 2 cut(s) 780, 1272
PciSI GCTCTTC 1 cut(s) 1292
PcsI WCGNNNNNNNCGW 1 cut(s) 86
PfeI GAWTC 3 cut(s) 342, 824, 1489
PkrI GCNGC 4 cut(s) 451, 516, 519, 574
PleI GAGTC 2 cut(s) 295, 412
PpsI GAGTC 2 cut(s) 295, 412
Psp124BI GAGCTC 1 cut(s) 1087
Psp1406I AACGTT 2 cut(s) 80, 89
Psp6I CCWGG 4 cut(s) 105, 209, 538, 762
PspGI CCWGG 4 cut(s) 105, 209, 538, 762
PspN4I GGNNCC 1 cut(s) 468
PspPI GGNCC 1 cut(s) 1444
PstI CTGCAG 2 cut(s) 454, 1155
PstNI CAGNNNCTG 1 cut(s) 505
PsuI RGATCY 1 cut(s) 751
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 6 cut(s) 233, 246, 761, 973, 1114, 1400
RsaNI GTAC 6 cut(s) 232, 245, 760, 972, 1113, 1399
RseI CAYNNNNRTG 1 cut(s) 792
SacI GAGCTC 1 cut(s) 1087
SapI GCTCTTC 1 cut(s) 1292
SaqAI TTAA 5 cut(s) 201, 293, 482, 731, 1178
SatI GCNGC 4 cut(s) 450, 515, 518, 573
Sau3AI GATC 4 cut(s) 382, 751, 861, 1231
Sau96I GGNCC 1 cut(s) 1444
SchI GAGTC 2 cut(s) 295, 413
ScrFI CCNGG 5 cut(s) 107, 211, 540, 661, 764
SduI GDGCHC 4 cut(s) 430, 661, 916, 1087
SfcI CTRYAG 3 cut(s) 450, 1140, 1151
Sfr274I CTCGAG 1 cut(s) 24
SinI GGWCC 1 cut(s) 1444
SlaI CTCGAG 1 cut(s) 24
SmiMI CAYNNNNRTG 1 cut(s) 792
SmlI CTYRAG 8 cut(s) 24, 186, 200, 292, 353, 438, 730, 743
SmoI CTYRAG 8 cut(s) 24, 186, 200, 292, 353, 438, 730, 743
SsiI CCGC 1 cut(s) 735
SspMI CTAG 2 cut(s) 1104, 1427
SstI GAGCTC 1 cut(s) 1087
StyD4I CCNGG 5 cut(s) 105, 209, 538, 659, 762
StyI CCWWGG 2 cut(s) 523, 1000
TaaI ACNGT 6 cut(s) 124, 549, 1117, 1148, 1376, 1403
TaiI ACGT 4 cut(s) 83, 92, 913, 1259
TaqI TCGA 4 cut(s) 18, 25, 249, 265
TatI WGTACW 3 cut(s) 244, 971, 1398
TfiI GAWTC 3 cut(s) 342, 824, 1489
Tru1I TTAA 5 cut(s) 201, 293, 482, 731, 1178
Tru9I TTAA 5 cut(s) 201, 293, 482, 731, 1178
TscAI CASTG 5 cut(s) 127, 896, 940, 994, 1527
TseFI GTSAC 5 cut(s) 846, 928, 1350, 1409, 1472
TseI GCWGC 4 cut(s) 449, 514, 517, 572
Tsp45I GTSAC 5 cut(s) 846, 928, 1350, 1409, 1472
TspDTI ATGAA 8 cut(s) 593, 769, 786, 1035, 1199, 1296, 1454, 1464
TspRI CASTG 5 cut(s) 127, 896, 940, 994, 1527
Vha464I CTTAAG 3 cut(s) 200, 292, 730
VneI GTGCAC 1 cut(s) 912
VpaK11BI GGWCC 1 cut(s) 1444
XapI RAATTY 4 cut(s) 64, 812, 1031, 1201
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XhoI CTCGAG 1 cut(s) 24
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 1104, 1427
ZraI GACGTC 1 cut(s) 1257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.