pycom15g36230
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009040
Physical Location & Seq
Reverse (-)
36273382 .. 36276600
3219 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2961 bp
ATGGCACTGTACTTGCTGCTGCCAACCGTATTCCTCTTCGGTTTGCTTTTCCCGCCATGTTTGCCTCTAACGGTCGGAAGTCAAACGGAAACTGAAGCCCTCCTTGAGTTGAAGAGGCAGCTCGAGGACCCTCTGAATGTTCTCGATTCCTGGAAAGAGACCGCTGACCGGTCGCCCTGCAACTTCTTCGGAGTTGCTTGTGATGGGATTAGCGGAAAAGTGATTGGAATTTCTCTTGATAACAAGTCCCTTTCGGGGGAGATTTCGCCCGCCATTGGTGTTCTTGACAGCCTAGAGACGCTTTCACTGCCTTCCAACAACATTACAGGAAAGCTTCCTGCCCAAGTCACTCTTTGCGCAAATCTTAAAACTATAAATCTCACCGGCAACAAAATGGTCGGAGCAATCCCCGATCTTTCTGCCCTCGGAAAATTGGAGATTCTTGATCTTTCTACAAACTTCTTCTCTGGCGCATTCCCATCCTGGGTGGGGAATCTGACCGGGTTGGTTTCTCTCGTGCTTGGGGAAAATGAGTTTGATGAAGGTGAAATTCCTGAGGGGCTTGAAAACTTGACCAACGTAACTTGGCTCCATCTGGGGTTTACCCAGTTGAGAGGAGAGATTCCGGAATCTGTTTACGAAATGAAGGCATTGGAGACGTTGGATATGTCGAGAAACAAGCTCTCTGGGAAGCTCTCGGAGTCCATTTCCCATCTTCAAAACCTCAATAAGATTGAACTTTTTCAAAACAATTTGACTGGAGAAATCCCACCAGGGCTTGCTAGTCTCACCCTCCTAAGGGAATTTGATATCTCATCAAACAAGTTTTATGGGAAGTTGCCTTCGGAGATGGGAAATCTCAAGAACTTGAAGGTTTTTCAGTTATTTGGGAACAATTTCTCCGGGGAATTCCCTGCTGGGTTCGGCGATATGAAACACCTTGGATCAGTCTCAATCTACGGAAACAGATTTTCTGGGGAATTTCCAACCAATTTTGGTAGATTCTCACCTTTGGAAAGCATTGACATATCCGAGAATCAGTTTTCAGGTGGTTTCCCAAGGTTTCTTTGTGAAAACGGCAGGTTAAATTTCTTGCTTGCTTTGGACAACAATTTTTCTGGGGAGTTTCCAGATTCTTATGTTCGTTGTAAATCTCTACAGCGGCTGAGAGTCAATCAGAACCGTTTGTTTGGGAAGATTCCAGCCGAATTTTGGTCACTTCCCTGCGCAACAATGATTGATTTTAGTGATAACAACTTCAGCGGAGAATTATCCCCCAGCATTGGGTTTTCTACCAGCTTGAATCAGTTGATATTGCAGAACAATGGTTTCTCAGGTTATCTTCCACCGCAACTTGGGGAACTATCGAACTTGGAGAAGCTCTATCTGAGCAGTAACAACTTTTCTGGTGAAATTCCTTCTGAAATGGGTGCTCTAAAGCAGCTGTCATCTTTGCATCTCGAATTCAATTCTTTGACGGGATCGATACCTTCAGAATTGGGAAACTGTGTTAAGTTGGTGGACGTGAATCTTGCTTCGAATTCTTTAACTGGTAATATCCCTCGTACATTATCACTAATGAGCTCTTTGAACTCTCTAAATGTCTCGAAAAACCACCTCATGGGTTTTATTCCTGAAGCTTTGGCGAAACTGAAGCTAAGTTCTATAGATTTTTCTGGGAATCATTTATCTGGAAGAGTTCCATCTGATCTTCTGACCATGGGAGGAGACAAAGCCTTCGCCGGAAACAAGGGACTCTGCATCGATCAATATTCCAGAACTCGCACAAATTCTGGGATGAACATGTGTACTGAAAAGCCGAATAAAAAAAGGGTGCTTGAAAATAAACTGGTTTTGTTCTTTATTATAGCATCGGCGTTGGTTGCTGCTTTTGCTGGATTACTGCTTGTCAGTTATAAGAATTTCAAGCTTGGGAAGGCGGTTGACAGAGAAAATAATTTGGAAGCGGGGAAGGAGATAGATCCGAAATGGAAACTTGCATCTTTCCACCAGTTGGAAATAGATGCAGATGAGATATGCGATTTGGAAGAAGAAAATTTGATTGGAAGTGGCAGTACAGGGAAAGTTTACCGCATAGATTTGAAGAAAGGTCGTGGCACGGTAGCTGTCAAACAACTTTGGAAAGAAGATGGCATGAAGCCTTTGATTGCAGAGATGGACATTTTGGGGAAGATTAGGCATAAAAATATACTGAAGCTATACGCTTGTTTAGTGAAGGGCGGATCTAGTGTTCTCGTGTTTGAGTACATGGCAAATGGTAATTTGTTTGAAGCGCTTCACAGAGAGATTAAATGCGGGAAGCCCGAATTGGATTGGTGCCAGAGGTATAAGATTGCTTTGGGAGCAGCTCGGGGGATTGCTTATCTGCACCATGATTGTTCACCAGCTATAATTCACAGAGATATTAAATCCACCAACATTCTGCTCGACGACGATTATGAGGCAAAAGTTGCTGATTTTGGGGTTGCAAAGATTGCAGAGAATTCTCAGAAGGGTTCTGATTACAGCTCATTTGCCGGCACACATGGCTATATTGCTCCCGAGCTGGCATATACTGCTAAAGTGAACGAAAAATGCGATGTGTATAGCTTCGGTGTTGTGCTGCTAGAGCTAGTAACTGGTAGGAGACCAATTGAAGAGGATTACGGGGAAGGAAAAGATATTGTTTACTGGGTTTCAACGCATCTCAGTGACCGTGAAGCGGTTGTTAAGATTCTCGATGAAAAAGTGGCAGATGAAAGTGTCCAGGATGACATGATCAAGGTCCTGAAGGTTGCTGTTCTTTGTACTACGAAGCTACCATCGTTGCGCCCGACAATGCGGGAGGTTATAAAGATGCTTGCGGATGCTGATCCTTGCAGTTTCCGGTCCAGGAACAATACCGGTAACGACAAAAAAGTCAAGGATTTCTCACCCAGTACCGATGAGTTGCTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

987

Amino Acids

108.37

Weight (kDa)

5.44

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 68 1.4e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 435 - 538 8.1e-06 Leucine-rich repeat region
Pkinase PF00069 683 - 952 4.9e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 684 - 954 3.2e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1917, 2852
AasI GACNNNNNNGTC 1 cut(s) 2918
Acc16I TGCGCA 2 cut(s) 358, 1228
Acc36I ACCTGC 1 cut(s) 1071
AccB1I GGYRCC 1 cut(s) 2337
AccB7I CCANNNNNTGG 2 cut(s) 1621, 2014
AccIII TCCGGA 1 cut(s) 625
AclWI GGATC 5 cut(s) 952, 1489, 1976, 2251, 2867
AcuI CTGAAG 7 cut(s) 114, 1243, 1476, 1656, 1673, 2234, 2810
AfaI GTAC 7 cut(s) 11, 1567, 1810, 2077, 2267, 2809, 2941
AfeI AGCGCT 1 cut(s) 2295
AflIII ACRYGT 1 cut(s) 1803
AgeI ACCGGT 2 cut(s) 168, 2903
AjiI CACGTC 1 cut(s) 1525
AjnI CCWGG 5 cut(s) 149, 482, 772, 2766, 2891
AjuI GAANNNNNNNTTGG 4 cut(s) 2046, 2078, 2312, 2344
AloI GAACNNNNNNTCC 4 cut(s) 884, 916, 2235, 2267
Alw21I GWGCWC 2 cut(s) 1435, 1586
Alw26I GTCTC 8 cut(s) 152, 290, 650, 791, 955, 1609, 1722, 2641
AlwI GGATC 5 cut(s) 952, 1489, 1976, 2251, 2867
AlwNI CAGNNNCTG 1 cut(s) 1165
Ama87I CYCGRG 3 cut(s) 122, 2370, 2561
Aor13HI TCCGGA 1 cut(s) 625
Aor51HI AGCGCT 1 cut(s) 2295
ApeKI GCWGC 7 cut(s) 16, 19, 118, 1441, 1886, 2366, 2623
ArsI GACNNNNNNTTYG 2 cut(s) 1721, 1753
AsiGI ACCGGT 2 cut(s) 168, 2903
Asp700I GAANNNNTTC 3 cut(s) 741, 896, 2295
AspLEI GCGC 5 cut(s) 359, 473, 1229, 2296, 2832
AspS9I GGNCC 3 cut(s) 127, 2785, 2889
AsuC2I CCSGG 2 cut(s) 502, 904
AsuHPI GGTGA 7 cut(s) 373, 557, 781, 999, 1421, 2394, 2925
AsuII TTCGAA 1 cut(s) 1538
AvaI CYCGRG 3 cut(s) 122, 2370, 2561
AvaII GGWCC 3 cut(s) 127, 2785, 2889
AxyI CCTNAGG 2 cut(s) 555, 797
BanI GGYRCC 1 cut(s) 2337
BanII GRGCYC 1 cut(s) 1586
BarI GAAGNNNNNNTAC 2 cut(s) 2059, 2091
BauI CACGAG 2 cut(s) 515, 2255
Bbv12I GWGCWC 2 cut(s) 1435, 1586
BbvI GCAGC 7 cut(s) 3, 6, 130, 1453, 1873, 2378, 2610
BccI CCATC 9 cut(s) 197, 487, 600, 720, 844, 1711, 2144, 2170, 2830
BceAI ACGGC 1 cut(s) 1093
BcgI CGANNNNNNTGC 4 cut(s) 169, 203, 401, 435
BciT130I CCWGG 5 cut(s) 151, 484, 774, 2768, 2893
BclI TGATCA 1 cut(s) 2778
BcnI CCSGG 2 cut(s) 502, 904
BcoDI GTCTC 8 cut(s) 152, 290, 650, 791, 955, 1609, 1722, 2641
BfaI CTAG 5 cut(s) 293, 783, 2247, 2627, 2633
BfmI CTRYAG 2 cut(s) 1157, 1665
BfoI RGCGCY 1 cut(s) 2297
BfuAI ACCTGC 1 cut(s) 1071
BisI GCNGC 8 cut(s) 17, 20, 119, 1163, 1442, 1887, 2367, 2624
BlsI GCNGC 8 cut(s) 18, 21, 120, 1164, 1443, 1888, 2368, 2625
Bme1390I CCNGG 7 cut(s) 151, 484, 502, 774, 904, 2768, 2893
Bme18I GGWCC 3 cut(s) 127, 2785, 2889
BmeT110I CYCGRG 3 cut(s) 122, 2370, 2561
BmgBI CACGTC 1 cut(s) 1525
BmgT120I GGNCC 3 cut(s) 127, 2785, 2889
BmiI GGNNCC 3 cut(s) 129, 590, 2339
BmrFI CCNGG 7 cut(s) 151, 484, 502, 774, 904, 2768, 2893
BmrI ACTGGG 3 cut(s) 601, 2701, 2931
BmsI GCATC 8 cut(s) 1465, 1770, 1880, 2009, 2014, 2713, 2847, 2857
BmuI ACTGGG 3 cut(s) 601, 2701, 2931
BpmI CTGGAG 1 cut(s) 780
Bpu14I TTCGAA 1 cut(s) 1538
BpuEI CTTGAG 2 cut(s) 125, 845
BpuMI CCSGG 2 cut(s) 502, 904
Bsa29I ATCGAT 2 cut(s) 1484, 1764
BsaBI GATNNNNATC 1 cut(s) 2871
BsaI GGTCTC 2 cut(s) 152, 2641
BsaJI CCNNGG 7 cut(s) 424, 483, 773, 903, 940, 1058, 1719
BsaWI WCCGGW 4 cut(s) 168, 625, 2886, 2903
BsaXI ACNNNNNCTCC 2 cut(s) 884, 914
Bse118I RCCGGY 4 cut(s) 168, 383, 2537, 2903
Bse1I ACTGG 8 cut(s) 607, 763, 1555, 1854, 2011, 2644, 2696, 2937
Bse21I CCTNAGG 2 cut(s) 555, 797
Bse8I GATNNNNATC 1 cut(s) 2871
BseAI TCCGGA 1 cut(s) 625
BseBI CCWGG 5 cut(s) 151, 484, 774, 2768, 2893
BseCI ATCGAT 2 cut(s) 1484, 1764
BseDI CCNNGG 7 cut(s) 424, 483, 773, 903, 940, 1058, 1719
BseGI GGATG 4 cut(s) 479, 1803, 2776, 2872
BseJI GATNNNNATC 1 cut(s) 2871
BseMII CTCAG 6 cut(s) 546, 1157, 1347, 1379, 2522, 2722
BseNI ACTGG 8 cut(s) 607, 763, 1555, 1854, 2011, 2644, 2696, 2937
BseRI GAGGAG 2 cut(s) 630, 1740
BseXI GCAGC 7 cut(s) 3, 6, 130, 1453, 1873, 2378, 2610
BseYI CCCAGC 2 cut(s) 917, 1277
BsgI GTGCAG 1 cut(s) 2372
Bsh1285I CGRYCG 2 cut(s) 75, 173
BshNI GGYRCC 1 cut(s) 2337
BshTI ACCGGT 2 cut(s) 168, 2903
BshVI ATCGAT 2 cut(s) 1484, 1764
BsiEI CGRYCG 2 cut(s) 75, 173
BsiHKAI GWGCWC 2 cut(s) 1435, 1586
BsiHKCI CYCGRG 3 cut(s) 122, 2370, 2561
BsiSI CCGG 9 cut(s) 169, 384, 501, 626, 903, 1743, 2538, 2887, 2904
BslFI GGGAC 2 cut(s) 232, 1767
BsmAI GTCTC 8 cut(s) 152, 290, 650, 791, 955, 1609, 1722, 2641
BsmBI CGTCTC 2 cut(s) 290, 650
BsmFI GGGAC 2 cut(s) 232, 1767
BsmI GAATGC 1 cut(s) 473
Bso31I GGTCTC 2 cut(s) 152, 2641
BsoBI CYCGRG 3 cut(s) 122, 2370, 2561
Bsp119I TTCGAA 1 cut(s) 1538
Bsp1286I GDGCHC 2 cut(s) 1435, 1586
Bsp13I TCCGGA 1 cut(s) 625
Bsp19I CCATGG 1 cut(s) 1719
BspCNI CTCAG 6 cut(s) 547, 1158, 1346, 1380, 2521, 2721
BspDI ATCGAT 2 cut(s) 1484, 1764
BspEI TCCGGA 1 cut(s) 625
BspLI GGNNCC 3 cut(s) 129, 590, 2339
BspMI ACCTGC 1 cut(s) 1071
BspPI GGATC 5 cut(s) 952, 1489, 1976, 2251, 2867
BspT104I TTCGAA 1 cut(s) 1538
BspT107I GGYRCC 1 cut(s) 2337
BspTNI GGTCTC 2 cut(s) 152, 2641
BsrFI RCCGGY 4 cut(s) 168, 383, 2537, 2903
BsrI ACTGG 8 cut(s) 607, 763, 1555, 1854, 2011, 2644, 2696, 2937
BssAI RCCGGY 4 cut(s) 168, 383, 2537, 2903
BssECI CCNNGG 7 cut(s) 424, 483, 773, 903, 940, 1058, 1719
BssSI CACGAG 2 cut(s) 515, 2255
BssT1I CCWWGG 3 cut(s) 940, 1058, 1719
Bst2BI CACGAG 2 cut(s) 515, 2255
Bst2UI CCWGG 5 cut(s) 151, 484, 774, 2768, 2893
Bst4CI ACNGT 7 cut(s) 9, 28, 73, 1184, 1508, 2122, 2717
Bst6I CTCTTC 4 cut(s) 41, 107, 1690, 2652
BstAPI GCANNNNNTGC 3 cut(s) 2471, 2495, 2576
BstBI TTCGAA 1 cut(s) 1538
BstC8I GCNNGC 6 cut(s) 270, 780, 1098, 2539, 2568, 2862
BstDEI CTNAG 8 cut(s) 555, 797, 1166, 1333, 1388, 1658, 2508, 2708
BstDSI CCRYGG 1 cut(s) 1719
BstF5I GGATG 4 cut(s) 479, 1803, 2776, 2872
BstH2I RGCGCY 1 cut(s) 2297
BstHHI GCGC 5 cut(s) 359, 473, 1229, 2296, 2832
BstMAI GTCTC 8 cut(s) 152, 290, 650, 791, 955, 1609, 1722, 2641
BstMCI CGRYCG 2 cut(s) 75, 173
BstNI CCWGG 5 cut(s) 151, 484, 774, 2768, 2893
BstNSI RCATGY 1 cut(s) 1807
BstSCI CCNGG 7 cut(s) 149, 482, 500, 772, 902, 2766, 2891
BstSFI CTRYAG 2 cut(s) 1157, 1665
BstV1I GCAGC 7 cut(s) 3, 6, 130, 1453, 1873, 2378, 2610
BstX2I RGATCY 2 cut(s) 1981, 2243
BstYI RGATCY 2 cut(s) 1981, 2243
Bsu15I ATCGAT 2 cut(s) 1484, 1764
Bsu36I CCTNAGG 2 cut(s) 555, 797
BsuTUI ATCGAT 2 cut(s) 1484, 1764
BtgI CCRYGG 1 cut(s) 1719
BtgZI GCGATG 1 cut(s) 2613
BtrI CACGTC 1 cut(s) 1525
BtsCI GGATG 4 cut(s) 479, 1803, 2776, 2872
BtsI GCAGTG 1 cut(s) 305
BtsIMutI CAGTG 3 cut(s) 5, 305, 2716
BveI ACCTGC 1 cut(s) 1071
Cac8I GCNNGC 6 cut(s) 270, 780, 1098, 2539, 2568, 2862
CaiI CAGNNNCTG 1 cut(s) 1165
CfoI GCGC 5 cut(s) 359, 473, 1229, 2296, 2832
Cfr10I RCCGGY 4 cut(s) 168, 383, 2537, 2903
Cfr13I GGNCC 3 cut(s) 127, 2785, 2889
ClaI ATCGAT 2 cut(s) 1484, 1764
CseI GACGC 1 cut(s) 307
Csp6I GTAC 7 cut(s) 10, 1566, 1809, 2076, 2266, 2808, 2940
CspAI ACCGGT 2 cut(s) 168, 2903
CviAII CATG 9 cut(s) 57, 1621, 1720, 1804, 2155, 2269, 2393, 2546, 2776
CviQI GTAC 7 cut(s) 10, 1566, 1809, 2076, 2266, 2808, 2940
DdeI CTNAG 8 cut(s) 555, 797, 1166, 1333, 1388, 1658, 2508, 2708
DrdI GACNNNNNNGTC 1 cut(s) 2918
DseDI GACNNNNNNGTC 1 cut(s) 2918
Eam1104I CTCTTC 4 cut(s) 41, 107, 1690, 2652
EarI CTCTTC 4 cut(s) 41, 107, 1690, 2652
EciI GGCGGA 1 cut(s) 2256
Ecl136II GAGCTC 1 cut(s) 1584
Eco130I CCWWGG 3 cut(s) 940, 1058, 1719
Eco24I GRGCYC 1 cut(s) 1586
Eco31I GGTCTC 2 cut(s) 152, 2641
Eco32I GATATC 1 cut(s) 811
Eco47I GGWCC 3 cut(s) 127, 2785, 2889
Eco47III AGCGCT 1 cut(s) 2295
Eco53kI GAGCTC 1 cut(s) 1584
Eco57I CTGAAG 7 cut(s) 114, 1243, 1476, 1656, 1673, 2234, 2810
Eco81I CCTNAGG 2 cut(s) 555, 797
Eco88I CYCGRG 3 cut(s) 122, 2370, 2561
EcoICRI GAGCTC 1 cut(s) 1584
EcoO109I RGGNCCY 2 cut(s) 127, 2785
EcoRI GAATTC 4 cut(s) 908, 1463, 1540, 2503
EcoRII CCWGG 5 cut(s) 149, 482, 772, 2766, 2891
EcoRV GATATC 1 cut(s) 811
EcoT14I CCWWGG 3 cut(s) 940, 1058, 1719
EcoT38I GRGCYC 1 cut(s) 1586
ErhI CCWWGG 3 cut(s) 940, 1058, 1719
Esp3I CGTCTC 2 cut(s) 290, 650
FaeI CATG 9 cut(s) 60, 1624, 1723, 1807, 2158, 2272, 2396, 2549, 2779
FalI AAGNNNNNCTT 4 cut(s) 87, 119, 336, 368
FaqI GGGAC 2 cut(s) 232, 1767
FatI CATG 9 cut(s) 56, 1620, 1719, 1803, 2154, 2268, 2392, 2545, 2775
FauI CCCGC 5 cut(s) 60, 277, 1960, 2309, 2835
FbaI TGATCA 1 cut(s) 2778
Fnu4HI GCNGC 8 cut(s) 17, 20, 119, 1163, 1442, 1887, 2367, 2624
FokI GGATG 4 cut(s) 466, 1810, 2783, 2879
FriOI GRGCYC 1 cut(s) 1586
Fsp4HI GCNGC 8 cut(s) 17, 20, 119, 1163, 1442, 1887, 2367, 2624
FspBI CTAG 5 cut(s) 293, 783, 2247, 2627, 2633
FspI TGCGCA 2 cut(s) 358, 1228
GlaI GCGC 5 cut(s) 358, 472, 1228, 2295, 2831
GluI GCNGC 8 cut(s) 17, 20, 119, 1163, 1442, 1887, 2367, 2624
GsaI CCCAGC 2 cut(s) 921, 1281
GsuI CTGGAG 1 cut(s) 780
HaeII RGCGCY 1 cut(s) 2297
HapII CCGG 9 cut(s) 169, 384, 501, 626, 903, 1743, 2538, 2887, 2904
HgaI GACGC 1 cut(s) 307
HhaI GCGC 5 cut(s) 359, 473, 1229, 2296, 2832
Hin1II CATG 9 cut(s) 60, 1624, 1723, 1807, 2158, 2272, 2396, 2549, 2779
Hin6I GCGC 5 cut(s) 357, 471, 1227, 2294, 2830
HinP1I GCGC 5 cut(s) 357, 471, 1227, 2294, 2830
HincII GTYRAC 1 cut(s) 1945
HindII GTYRAC 1 cut(s) 1945
HindIII AAGCTT 3 cut(s) 332, 1638, 1928
HpaII CCGG 9 cut(s) 169, 384, 501, 626, 903, 1743, 2538, 2887, 2904
HphI GGTGA 7 cut(s) 373, 557, 781, 999, 1421, 2394, 2925
Hpy166II GTNNAC 9 cut(s) 603, 637, 1522, 1809, 1945, 2089, 2402, 2587, 2689
Hpy8I GTNNAC 9 cut(s) 603, 637, 1522, 1809, 1945, 2089, 2402, 2587, 2689
Hpy99I CGWCG 2 cut(s) 2453, 2456
HpyCH4III ACNGT 7 cut(s) 9, 28, 73, 1184, 1508, 2122, 2717
HpyCH4IV ACGT 3 cut(s) 579, 659, 1524
HpyF3I CTNAG 8 cut(s) 555, 797, 1166, 1333, 1388, 1658, 2508, 2708
HpySE526I ACGT 3 cut(s) 579, 659, 1524
Hsp92II CATG 9 cut(s) 60, 1624, 1723, 1807, 2158, 2272, 2396, 2549, 2779
HspAI GCGC 5 cut(s) 357, 471, 1227, 2294, 2830
Kpn2I TCCGGA 1 cut(s) 625
KroI GCCGGC 1 cut(s) 2537
KroNI GCCGGC 1 cut(s) 2539
Ksp22I TGATCA 1 cut(s) 2778
LmnI GCTCC 4 cut(s) 401, 594, 2363, 2563
Lsp1109I GCAGC 7 cut(s) 3, 6, 130, 1453, 1873, 2378, 2610
LweI GCATC 8 cut(s) 1465, 1770, 1880, 2009, 2014, 2713, 2847, 2857
MaeI CTAG 5 cut(s) 293, 783, 2247, 2627, 2633
MaeII ACGT 3 cut(s) 579, 659, 1524
MaeIII GTNAC 7 cut(s) 346, 580, 1215, 1394, 2635, 2711, 2906
MfeI CAATTG 1 cut(s) 2652
MflI RGATCY 2 cut(s) 1981, 2243
MhlI GDGCHC 2 cut(s) 1435, 1586
MlyI GAGTC 3 cut(s) 710, 1179, 1749
MmeI TCCRAC 6 cut(s) 55, 339, 379, 642, 1010, 1995
MroI TCCGGA 1 cut(s) 625
MroNI GCCGGC 1 cut(s) 2537
MroXI GAANNNNTTC 3 cut(s) 741, 896, 2295
MseI TTAA 7 cut(s) 366, 1085, 1512, 1547, 2310, 2427, 2730
MslI CAYNNNNRTG 1 cut(s) 2709
MspA1I CMGCKG 4 cut(s) 164, 1162, 1263, 1444
MspI CCGG 9 cut(s) 169, 384, 501, 626, 903, 1743, 2538, 2887, 2904
MspR9I CCNGG 7 cut(s) 151, 484, 502, 774, 904, 2768, 2893
MunI CAATTG 1 cut(s) 2652
Mva1269I GAATGC 1 cut(s) 473
MvaI CCWGG 5 cut(s) 151, 484, 774, 2768, 2893
NaeI GCCGGC 1 cut(s) 2539
NciI CCSGG 2 cut(s) 502, 904
NcoI CCATGG 1 cut(s) 1719
NgoMIV GCCGGC 1 cut(s) 2537
NlaIII CATG 9 cut(s) 60, 1624, 1723, 1807, 2158, 2272, 2396, 2549, 2779
NlaIV GGNNCC 3 cut(s) 129, 590, 2339
NmuCI GTSAC 3 cut(s) 346, 1215, 2711
NsbI TGCGCA 2 cut(s) 358, 1228
NspI RCATGY 1 cut(s) 1807
NspV TTCGAA 1 cut(s) 1538
PaeR7I CTCGAG 1 cut(s) 122
PciI ACATGT 1 cut(s) 1803
PcsI WCGNNNNNNNCGW 2 cut(s) 2595, 2831
PctI GAATGC 1 cut(s) 473
PdiI GCCGGC 1 cut(s) 2539
PdmI GAANNNNTTC 3 cut(s) 741, 896, 2295
PflMI CCANNNNNTGG 2 cut(s) 1621, 2014
PfoI TCCNGGA 3 cut(s) 149, 2766, 2891
PinAI ACCGGT 2 cut(s) 168, 2903
PkrI GCNGC 8 cut(s) 18, 21, 120, 1164, 1443, 1888, 2368, 2625
PleI GAGTC 3 cut(s) 709, 1178, 1749
PpsI GAGTC 3 cut(s) 709, 1178, 1749
PpuMI RGGWCCY 2 cut(s) 127, 2785
PscI ACATGT 1 cut(s) 1803
PsiI TTATAA 2 cut(s) 1917, 2852
Psp124BI GAGCTC 1 cut(s) 1586
Psp5II RGGWCCY 2 cut(s) 127, 2785
Psp6I CCWGG 5 cut(s) 149, 482, 772, 2766, 2891
PspFI CCCAGC 2 cut(s) 917, 1277
PspGI CCWGG 5 cut(s) 149, 482, 772, 2766, 2891
PspN4I GGNNCC 3 cut(s) 129, 590, 2339
PspPI GGNCC 3 cut(s) 127, 2785, 2889
PspPPI RGGWCCY 2 cut(s) 127, 2785
PspXI VCTCGAGB 1 cut(s) 122
PstNI CAGNNNCTG 1 cut(s) 1165
PsuI RGATCY 2 cut(s) 1981, 2243
PvuII CAGCTG 1 cut(s) 1444
RsaI GTAC 7 cut(s) 11, 1567, 1810, 2077, 2267, 2809, 2941
RsaNI GTAC 7 cut(s) 10, 1566, 1809, 2076, 2266, 2808, 2940
RseI CAYNNNNRTG 1 cut(s) 2709
SacI GAGCTC 1 cut(s) 1586
SaqAI TTAA 7 cut(s) 366, 1085, 1512, 1547, 2310, 2427, 2730
SatI GCNGC 8 cut(s) 17, 20, 119, 1163, 1442, 1887, 2367, 2624
Sau96I GGNCC 3 cut(s) 127, 2785, 2889
SchI GAGTC 3 cut(s) 710, 1179, 1749
ScrFI CCNGG 7 cut(s) 151, 484, 502, 774, 904, 2768, 2893
SduI GDGCHC 2 cut(s) 1435, 1586
SfaNI GCATC 8 cut(s) 1465, 1770, 1880, 2009, 2014, 2713, 2847, 2857
SfcI CTRYAG 2 cut(s) 1157, 1665
Sfr274I CTCGAG 1 cut(s) 122
SfuI TTCGAA 1 cut(s) 1538
SinI GGWCC 3 cut(s) 127, 2785, 2889
SlaI CTCGAG 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 2709
SmlI CTYRAG 3 cut(s) 104, 122, 860
SmoI CTYRAG 3 cut(s) 104, 122, 860
SspI AATATT 1 cut(s) 1772
SspMI CTAG 5 cut(s) 293, 783, 2247, 2627, 2633
SstI GAGCTC 1 cut(s) 1586
StyD4I CCNGG 7 cut(s) 149, 482, 500, 772, 902, 2766, 2891
StyI CCWWGG 3 cut(s) 940, 1058, 1719
TaaI ACNGT 7 cut(s) 9, 28, 73, 1184, 1508, 2122, 2717
TaiI ACGT 3 cut(s) 582, 662, 1527
TatI WGTACW 5 cut(s) 9, 1808, 2075, 2265, 2807
TauI GCSGC 1 cut(s) 1165
Tru1I TTAA 7 cut(s) 366, 1085, 1512, 1547, 2310, 2427, 2730
Tru9I TTAA 7 cut(s) 366, 1085, 1512, 1547, 2310, 2427, 2730
TscAI CASTG 3 cut(s) 12, 312, 2716
TseFI GTSAC 3 cut(s) 346, 1215, 2711
TseI GCWGC 7 cut(s) 16, 19, 118, 1441, 1886, 2366, 2623
Tsp45I GTSAC 3 cut(s) 346, 1215, 2711
TspDTI ATGAA 7 cut(s) 555, 659, 947, 1814, 2171, 2757, 2772
TspGWI ACGGA 2 cut(s) 101, 975
TspRI CASTG 3 cut(s) 12, 312, 2716
Van91I CCANNNNNTGG 2 cut(s) 1621, 2014
VpaK11BI GGWCC 3 cut(s) 127, 2785, 2889
XceI RCATGY 1 cut(s) 1807
XcmI CCANNNNNNNNNTGG 1 cut(s) 1209
XhoI CTCGAG 1 cut(s) 122
XmnI GAANNNNTTC 3 cut(s) 741, 896, 2295
XspI CTAG 5 cut(s) 293, 783, 2247, 2627, 2633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.