pycom15g36320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013144
Physical Location & Seq
Reverse (-)
36362996 .. 36363616
621 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g36320.1

Sequence Viewer

Length: 621 bp
ATGTATAAAAATCCCCCCAACCCAACTCATGTGAATCGTGATGCCCCACTTCCATCCCTCACTTCGAAACCCAAAGCCGCTCACGCTCACCGTCGTTTCCGACAAATACGCTATGAAGTCAATCTCCTCGATTACCAGACTTCAACGGCCACGATCTCTCCCTCTCCCTCCCCTTTCCCAATCCTCGCCACGCCCATAATCATGCTCGCCGCCGCATCCTCCTCCACAACATGGCTCCGAGCTCACCGCATCCGCTACCTCTTCCTTCTCCTCTGCTCTCCGATTCTCATCCCTTTCCTCTGCGCCATCTTCCCACTGCTCTGTGCCGCCGAGCTTTGCCTACACCTCTGCCGCCGCCGTATAAAGATTTCTCACGATGAGGCCGGGGAGGAGGAGGAGCAGATGCGCCGGTGCGAAGAAGGACGCAGCAGTGGAAGTGCGGCGGCGGCGGCGGGGAAGGAAGACGGGAAAGAAGAGATGGGGCTGCTGCAGAGGTACTTGGAGGATCAGCTGCTGCTGGTTGGATCTGTGTATGATTGCAGGGACGATGATAGTGACGATTTGTATCACGAGATTCATGACTATGATAATGATTCCAACACTACTCCTCTTTTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

207

Amino Acids

23.18

Weight (kDa)

5.74

Isoelectric Point (pI)

61.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013144)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35430 AT4G09170
fragaria_vesca FvH4_5g31870
malus_domestica MD08G1218400.v1.1 MD15G1406800.v1.1
prunus_persica Prupe.1G555000_v2.0.a1
pyrus_communis pycom15g36320
rosa_chinensis RchiOBHm_Chr7g0227891
rosa_laevigata RLG00000001606
rosa_multiflora Rmu_co8313049.1_g000001 Rmu_sc0000347.1_g000006 Rmu_sc0005743.1_g000002
rosa_roxburghii Rroxscaffold_3G00231800
rosa_rugosa Rorug07G0244000
rosa_samantha Rh7AG397400 Rh7CG415600 Rh7DG392900
rosa_wichuraiana Rw7G033060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 231
AccBSI CCGCTC 1 cut(s) 80
AclWI GGATC 2 cut(s) 513, 532
AcoI YGGCCR 1 cut(s) 147
AfaI GTAC 1 cut(s) 497
AfiI CCNNNNNNNGG 1 cut(s) 231
AgsI TTSAA 1 cut(s) 144
AluBI AGCT 3 cut(s) 242, 334, 511
AluI AGCT 3 cut(s) 242, 334, 511
Alw21I GWGCWC 1 cut(s) 244
AlwI GGATC 2 cut(s) 513, 532
AlwNI CAGNNNCTG 1 cut(s) 514
AoxI GGCC 2 cut(s) 147, 381
ApeKI GCWGC 5 cut(s) 426, 484, 487, 511, 514
AspLEI GCGC 2 cut(s) 305, 408
AsuC2I CCSGG 1 cut(s) 385
AsuHPI GGTGA 2 cut(s) 80, 236
AsuII TTCGAA 1 cut(s) 65
BanII GRGCYC 1 cut(s) 244
BauI CACGAG 1 cut(s) 569
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 244
BbvI GCAGC 5 cut(s) 438, 471, 474, 498, 501
BccI CCATC 3 cut(s) 61, 314, 472
BceAI ACGGC 2 cut(s) 162, 342
BcnI CCSGG 1 cut(s) 385
BfmI CTRYAG 1 cut(s) 488
Bme1390I CCNGG 1 cut(s) 385
BmiI GGNNCC 1 cut(s) 236
BmrFI CCNGG 1 cut(s) 385
BmsI GCATC 4 cut(s) 31, 224, 258, 393
BpiI GAAGAC 1 cut(s) 468
Bpu14I TTCGAA 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 385
BsaBI GATNNNNATC 2 cut(s) 287, 564
BsaJI CCNNGG 1 cut(s) 384
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse118I RCCGGY 1 cut(s) 408
Bse8I GATNNNNATC 2 cut(s) 287, 564
BseDI CCNNGG 1 cut(s) 384
BseGI GGATG 4 cut(s) 53, 215, 249, 288
BseJI GATNNNNATC 2 cut(s) 287, 564
BseLI CCNNNNNNNGG 1 cut(s) 231
BseRI GAGGAG 7 cut(s) 116, 211, 260, 404, 407, 410, 597
BseXI GCAGC 5 cut(s) 438, 471, 474, 498, 501
BshFI GGCC 2 cut(s) 149, 383
BsiHKAI GWGCWC 1 cut(s) 244
BsiSI CCGG 2 cut(s) 384, 409
BslFI GGGAC 1 cut(s) 557
BslI CCNNNNNNNGG 1 cut(s) 231
BsmFI GGGAC 1 cut(s) 557
BsnI GGCC 2 cut(s) 149, 383
Bsp119I TTCGAA 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 244
Bsp143I GATC 3 cut(s) 153, 505, 524
BspANI GGCC 2 cut(s) 149, 383
BspHI TCATGA 1 cut(s) 577
BspLI GGNNCC 1 cut(s) 236
BspMAI CTGCAG 1 cut(s) 492
BspPI GGATC 2 cut(s) 513, 532
BspT104I TTCGAA 1 cut(s) 65
BsrBI CCGCTC 1 cut(s) 80
BsrFI RCCGGY 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 384
BssMI GATC 3 cut(s) 153, 505, 524
BssSI CACGAG 1 cut(s) 569
Bst2BI CACGAG 1 cut(s) 569
Bst4CI ACNGT 1 cut(s) 92
Bst6I CTCTTC 2 cut(s) 266, 468
BstBI TTCGAA 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 207
BstF5I GGATG 4 cut(s) 53, 215, 249, 288
BstHHI GCGC 2 cut(s) 305, 408
BstKTI GATC 3 cut(s) 156, 508, 527
BstMBI GATC 3 cut(s) 153, 505, 524
BstMWI GCNNNNNNNGC 3 cut(s) 83, 446, 449
BstSCI CCNGG 1 cut(s) 383
BstSFI CTRYAG 1 cut(s) 488
BstV1I GCAGC 5 cut(s) 438, 471, 474, 498, 501
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 524
BstYI RGATCY 1 cut(s) 524
BsuRI GGCC 2 cut(s) 149, 383
BtsCI GGATG 4 cut(s) 53, 215, 249, 288
BtsI GCAGTG 2 cut(s) 314, 436
BtsIMutI CAGTG 2 cut(s) 314, 436
Cac8I GCNNGC 1 cut(s) 207
CaiI CAGNNNCTG 1 cut(s) 514
CciI TCATGA 1 cut(s) 577
CfoI GCGC 2 cut(s) 305, 408
Cfr10I RCCGGY 1 cut(s) 408
CseI GACGC 1 cut(s) 432
Csp6I GTAC 1 cut(s) 496
CviAII CATG 4 cut(s) 29, 202, 231, 578
CviJI RGCY 9 cut(s) 77, 149, 235, 242, 334, 383, 484, 511, 617
CviKI_1 RGCY 9 cut(s) 77, 149, 235, 242, 334, 383, 484, 511, 617
CviQI GTAC 1 cut(s) 496
DpnI GATC 3 cut(s) 155, 507, 526
DpnII GATC 3 cut(s) 153, 505, 524
EaeI YGGCCR 1 cut(s) 147
Eam1104I CTCTTC 2 cut(s) 266, 468
EarI CTCTTC 2 cut(s) 266, 468
Ecl136II GAGCTC 1 cut(s) 242
Eco24I GRGCYC 1 cut(s) 244
Eco53kI GAGCTC 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 242
EcoT38I GRGCYC 1 cut(s) 244
FaeI CATG 4 cut(s) 32, 205, 234, 581
FaqI GGGAC 1 cut(s) 557
FatI CATG 4 cut(s) 28, 201, 230, 577
FauI CCCGC 1 cut(s) 445
FokI GGATG 4 cut(s) 40, 202, 236, 275
FriOI GRGCYC 1 cut(s) 244
GlaI GCGC 2 cut(s) 304, 407
HaeIII GGCC 2 cut(s) 149, 383
HapII CCGG 2 cut(s) 384, 409
HgaI GACGC 1 cut(s) 432
HhaI GCGC 2 cut(s) 305, 408
Hin1II CATG 4 cut(s) 32, 205, 234, 581
Hin6I GCGC 2 cut(s) 303, 406
HinP1I GCGC 2 cut(s) 303, 406
HinfI GANTC 4 cut(s) 34, 283, 574, 593
HpaII CCGG 2 cut(s) 384, 409
HphI GGTGA 2 cut(s) 80, 236
Hpy188I TCNGA 3 cut(s) 101, 239, 282
Hpy188III TCNNGA 4 cut(s) 38, 374, 569, 578
Hpy99I CGWCG 1 cut(s) 96
HpyAV CCTTC 3 cut(s) 275, 413, 451
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4V TGCA 2 cut(s) 490, 540
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 446, 449
Hsp92II CATG 4 cut(s) 32, 205, 234, 581
HspAI GCGC 2 cut(s) 303, 406
Kzo9I GATC 3 cut(s) 153, 505, 524
LmnI GCTCC 2 cut(s) 240, 397
LpnPI CCDG 5 cut(s) 149, 397, 422, 503, 526
Lsp1109I GCAGC 5 cut(s) 438, 471, 474, 498, 501
LweI GCATC 4 cut(s) 31, 224, 258, 393
MaeIII GTNAC 1 cut(s) 554
MalI GATC 3 cut(s) 155, 507, 526
MbiI CCGCTC 1 cut(s) 80
MboI GATC 3 cut(s) 153, 505, 524
MboII GAAGA 5 cut(s) 253, 301, 428, 473, 485
MflI RGATCY 1 cut(s) 524
MhlI GDGCHC 1 cut(s) 244
MmeI TCCRAC 3 cut(s) 124, 502, 621
MslI CAYNNNNRTG 2 cut(s) 200, 582
MspA1I CMGCKG 1 cut(s) 511
MspI CCGG 2 cut(s) 384, 409
MspR9I CCNGG 1 cut(s) 385
MwoI GCNNNNNNNGC 3 cut(s) 83, 446, 449
NciI CCSGG 1 cut(s) 385
NdeII GATC 3 cut(s) 153, 505, 524
NlaIII CATG 4 cut(s) 32, 205, 234, 581
NlaIV GGNNCC 1 cut(s) 236
NmeAIII GCCGAG 1 cut(s) 355
NmuCI GTSAC 1 cut(s) 554
NspV TTCGAA 1 cut(s) 65
PagI TCATGA 1 cut(s) 577
PfeI GAWTC 4 cut(s) 34, 283, 574, 593
PflMI CCANNNNNTGG 1 cut(s) 231
Psp124BI GAGCTC 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 236
PstI CTGCAG 1 cut(s) 492
PstNI CAGNNNCTG 1 cut(s) 514
PsuI RGATCY 1 cut(s) 524
PvuII CAGCTG 1 cut(s) 511
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
RseI CAYNNNNRTG 2 cut(s) 200, 582
SacI GAGCTC 1 cut(s) 244
Sau3AI GATC 3 cut(s) 153, 505, 524
ScrFI CCNGG 1 cut(s) 385
SduI GDGCHC 1 cut(s) 244
SetI ASST 6 cut(s) 244, 261, 336, 348, 497, 513
SfaNI GCATC 4 cut(s) 31, 224, 258, 393
SfcI CTRYAG 1 cut(s) 488
SfuI TTCGAA 1 cut(s) 65
SgrAI CRCCGGYG 1 cut(s) 408
SmiMI CAYNNNNRTG 2 cut(s) 200, 582
SstI GAGCTC 1 cut(s) 244
StyD4I CCNGG 1 cut(s) 383
TaaI ACNGT 1 cut(s) 92
TaqI TCGA 2 cut(s) 65, 129
TfiI GAWTC 4 cut(s) 34, 283, 574, 593
TscAI CASTG 2 cut(s) 321, 436
TseFI GTSAC 1 cut(s) 554
TseI GCWGC 5 cut(s) 426, 484, 487, 511, 514
Tsp45I GTSAC 1 cut(s) 554
TspDTI ATGAA 2 cut(s) 129, 566
TspRI CASTG 2 cut(s) 321, 436
Van91I CCANNNNNTGG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.