pycom15g38470

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007444
Physical Location & Seq
Reverse (-)
38255570 .. 38261565
5996 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2109 bp
ATGGCACTTAAGGCTCCAGCTCCGGAAGCTGCTGAGATTGCAATTGCATCGATTGGGCGTGGATACGATGTATCCACTGATTTAAGGCTCAAGTACTGTAAAGGGGAGTCTCACACTGCTCGATTGATTGAAATCGATGACGATGGAGGCCACGAGATTGTTCTTCCCGGTGGGATTACCATCCCAAATATTCCCAAATCGATTAAATGCGATAAAGGAGAGCGCACACGATTCAGGTCCGACGTTCTCTCTTTCCAACAGATGTCAGAGCAGTTCAATCAGGAAATATCGTTGACTGGAAAAATACCCTCAGGTCTCTTCAATTCTATGTTTGATTTCTCGGGTAGTTGGCAGAAAGATGCAGCCAACACTAAGACCCTTGCTTTTGATGGGGTGTTCATCACTCTCTATACTGTTGCACTGGAAAAATCTCAGATGGTACTGTGCGAGAATGTTATTAAAGCAGTCCCATCATCGTGGGAACCTGCTGCATTAGCAAGGTTTATAGAGAGATTTGGAACACATATTGTTGTTGGTGTGAAGATGGGAGGGAAAGATGTTATATATATGAAACAGCAGCATTCGTCAACTCTTCAACCGGCTGATATACAGAAAAAACTAAAGGATATGGCAGATAAAAGGTTTTTAGATGCCAATGGTCGGTATGGTATGCCTTCGGAACAAGGTTACAAGAATGGCAAGTTTGAAATCAGGGAGCAGCGGTTAAGGTTTGCAGATACTAGTCCATCAAGTTCTTATTCACACAAGGAAGTGCGTATAGACATTTTAATCATTTGCAAGAGGAGAGGTGGAAGTGAGATGAGAAATATATCCCATAATGAATGGTTACAAAGTGTTCAGTTGGAGCCTGATGTGATCACAATGTCATTTATACCAATTACTTCTCTGTTGAATGGAGTCCCTGGGAGTGGATTCTTGAGCCATGCCATAAATCTTTATTTACGATATAAACCACCAATTGAAGAACTCCATCAGTTTTTGGAGTTTCAGCTGCCAAGGCAATGGGCACCAGTTTTTAGTGACCTGCCCCTTGGTCCACAGCGGAAGCAACAAAGCAATGTATCTCTACAGTTTAGCTTAATGGGGCCCAAACTTTACGTCAACACCAGTCTGGTTGATGTGGGAAAGAGGCCAGTGACTGGTCTGCGACTGTATTTAGAGGGCAAAAGAAGCAACCGTTTGGCCATTCACTTACAACACCTCACCTCACTTCCAAATACCTTCCAACTCGAAGATGATCCAAATGGAAACTTTCGTCGCGATTCCTATGATCGTAAATACTATGAGAAAGTCCAGTGGAAGAACTTTTCCCATGTCTGCACTGCCCCTGTGGAGTCTGATGAGGATCTTTCAATAGTAACTGGAGCCCAGTTACAGGTTGAGAATTATGGAATAAAGAATATCCTCTTCTTGAGACTCCGCTTCTCTACTGTTTCTGGTGCGACAAAGGTAAAGCATCCTGAGTGGGATGGATCACCAGGCTTGGCACATAAGTCCGGGCTCATATCAACACTAATCAGCCATCATTTTACAACAGTTCAGAAACCAGCTCCACGGCCAGCAGATGTGAACATAAACTCTGCCGTTTACCCTGGAGGTCCTCCGGTTCCCATCCAAGCCCCAAAGCTTCGGAAATTCGTTGACACAACGGAGATGGTAAGAGGGCCACAGGAGACTCCGGGTTATTGGGTTGTCTCTGGGGCAAGGCTTGTGGTTGAGAAGGGCAGGATATCCCTCCGTGTGAAATACTCTCTACTGACTGTAATATTACCTGACGAAGAAGAGTTGGAGGGACAAACCGCCGGGGGGGGGGGGTTGGGTGTGGGAGATGTGGTATTCGATACCGTGAGAGTTGCCGAGGCTGTTGCGGTAGTGTTGCATATTTCATTTCTCACCTCCGATGTCGTTTGTTTTTGCAATGTAGATTTTTCTGACCTTCACAAGTTCAGAACAGGGCAGGGTTCTACCCTCCGCAAATTATATGGTGTAAATCTTTGGATGGGGTGTGTATCAAGAGCTGAGCTTCGGCTTTCACTAGCGACTAGGCGTCGCGAAAGGCACGGTGGCATGATGTCCAGATTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

703

Amino Acids

78.33

Weight (kDa)

9.09

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 106 - 320 8.6e-39 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 493, 1053
AccB1I GGYRCC 1 cut(s) 1027
AccB7I CCANNNNNTGG 1 cut(s) 1160
AccII CGCG 2 cut(s) 1281, 2073
AccIII TCCGGA 1 cut(s) 22
AciI CCGC 6 cut(s) 721, 1063, 1441, 1821, 1889, 1993
AclWI GGATC 3 cut(s) 1253, 1374, 1501
AcoI YGGCCR 2 cut(s) 1203, 1577
AcsI RAATTY 1 cut(s) 1655
AcyI GRCGYC 1 cut(s) 2068
AfaI GTAC 2 cut(s) 95, 441
AfiI CCNNNNNNNGG 6 cut(s) 660, 929, 1160, 1396, 1827, 1830
AflII CTTAAG 1 cut(s) 8
AgsI TTSAA 8 cut(s) 131, 277, 322, 596, 707, 913, 983, 1374
AhdI GACNNNNNGTC 1 cut(s) 2067
AhlI ACTAGT 1 cut(s) 740
AjnI CCWGG 3 cut(s) 922, 1498, 1612
AluBI AGCT 8 cut(s) 20, 29, 1012, 1098, 1571, 1648, 2039, 2044
AluI AGCT 8 cut(s) 20, 29, 1012, 1098, 1571, 1648, 2039, 2044
Alw26I GTCTC 5 cut(s) 114, 320, 1429, 1688, 1720
AlwI GGATC 3 cut(s) 1253, 1374, 1501
AlwNI CAGNNNCTG 1 cut(s) 1160
Ama87I CYCGRG 1 cut(s) 340
Aor13HI TCCGGA 1 cut(s) 22
AoxI GGCC 6 cut(s) 148, 1106, 1151, 1203, 1577, 1685
ApaI GGGCCC 1 cut(s) 1110
ApeKI GCWGC 6 cut(s) 29, 362, 488, 577, 718, 1012
ApoI RAATTY 1 cut(s) 1655
ArsI GACNNNNNNTTYG 2 cut(s) 1104, 1136
AspLEI GCGC 1 cut(s) 225
AspS9I GGNCC 6 cut(s) 237, 1055, 1106, 1107, 1619, 1685
AsuC2I CCSGG 4 cut(s) 168, 1519, 1701, 1825
AsuHPI GGTGA 3 cut(s) 1216, 1488, 1906
AvaI CYCGRG 1 cut(s) 340
AvaII GGWCC 3 cut(s) 237, 1055, 1619
AxyI CCTNAGG 1 cut(s) 310
BaeGI GKGCMC 2 cut(s) 1030, 1110
BalI TGGCCA 1 cut(s) 1205
BanI GGYRCC 1 cut(s) 1027
BanII GRGCYC 3 cut(s) 1110, 1390, 1524
BauI CACGAG 1 cut(s) 152
BbvI GCAGC 6 cut(s) 16, 374, 475, 589, 730, 999
BceAI ACGGC 2 cut(s) 1589, 1592
BcgI CGANNNNNNTGC 4 cut(s) 30, 64, 1868, 1902
BciT130I CCWGG 3 cut(s) 924, 1500, 1614
BciVI GTATCC 2 cut(s) 56, 82
BclI TGATCA 1 cut(s) 876
BcnI CCSGG 4 cut(s) 168, 1519, 1701, 1825
BcoDI GTCTC 5 cut(s) 114, 320, 1429, 1688, 1720
BcuI ACTAGT 1 cut(s) 740
BfaI CTAG 3 cut(s) 741, 2057, 2064
BfmI CTRYAG 1 cut(s) 1088
BfrI CTTAAG 1 cut(s) 8
BfuAI ACCTGC 2 cut(s) 493, 1053
BfuI GTATCC 2 cut(s) 56, 82
BisI GCNGC 6 cut(s) 30, 363, 489, 578, 719, 1013
BlpI GCTNAGC 1 cut(s) 2040
BlsI GCNGC 6 cut(s) 31, 364, 490, 579, 720, 1014
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 7 cut(s) 168, 924, 1500, 1519, 1614, 1701, 1825
Bme18I GGWCC 3 cut(s) 237, 1055, 1619
BmeRI GACNNNNNGTC 1 cut(s) 2067
BmeT110I CYCGRG 1 cut(s) 340
BmgT120I GGNCC 6 cut(s) 237, 1055, 1106, 1107, 1619, 1685
BmiI GGNNCC 8 cut(s) 15, 483, 867, 1029, 1107, 1108, 1387, 1629
BmrFI CCNGG 7 cut(s) 168, 924, 1500, 1519, 1614, 1701, 1825
BmrI ACTGGG 1 cut(s) 1384
BmsI GCATC 4 cut(s) 56, 349, 640, 1486
BmuI ACTGGG 1 cut(s) 1384
BpmI CTGGAG 2 cut(s) 1404, 1635
Bpu1102I GCTNAGC 1 cut(s) 2040
BpuEI CTTGAG 3 cut(s) 74, 958, 1453
BpuMI CCSGG 4 cut(s) 168, 1519, 1701, 1825
Bsa29I ATCGAT 3 cut(s) 50, 135, 200
BsaBI GATNNNNATC 3 cut(s) 131, 179, 1365
BsaHI GRCGYC 1 cut(s) 2068
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 8 cut(s) 922, 923, 1016, 1051, 1574, 1612, 1824, 1878
BsaWI WCCGGW 2 cut(s) 22, 1624
BsaXI ACNNNNNCTCC 4 cut(s) 707, 737, 1839, 1869
Bsc4I CCNNNNNNNGG 6 cut(s) 660, 929, 1160, 1396, 1827, 1830
Bse118I RCCGGY 1 cut(s) 598
Bse1I ACTGG 9 cut(s) 301, 426, 1031, 1128, 1154, 1165, 1315, 1387, 1390
Bse21I CCTNAGG 1 cut(s) 310
Bse3DI GCAATG 3 cut(s) 1028, 1084, 1945
Bse8I GATNNNNATC 3 cut(s) 131, 179, 1365
BseAI TCCGGA 1 cut(s) 22
BseBI CCWGG 3 cut(s) 924, 1500, 1614
BseCI ATCGAT 3 cut(s) 50, 135, 200
BseDI CCNNGG 8 cut(s) 922, 923, 1016, 1051, 1574, 1612, 1824, 1878
BseGI GGATG 5 cut(s) 180, 1477, 1495, 1632, 2025
BseJI GATNNNNATC 3 cut(s) 131, 179, 1365
BseLI CCNNNNNNNGG 6 cut(s) 660, 929, 1160, 1396, 1827, 1830
BseMI GCAATG 3 cut(s) 1028, 1084, 1945
BseMII CTCAG 5 cut(s) 24, 324, 446, 1473, 2031
BseNI ACTGG 9 cut(s) 301, 426, 1031, 1128, 1154, 1165, 1315, 1387, 1390
BseRI GAGGAG 1 cut(s) 817
BseSI GKGCMC 2 cut(s) 1030, 1110
BseXI GCAGC 6 cut(s) 16, 374, 475, 589, 730, 999
BsgI GTGCAG 1 cut(s) 1324
Bsh1236I CGCG 2 cut(s) 1281, 2073
BshFI GGCC 6 cut(s) 150, 1108, 1153, 1205, 1579, 1687
BshNI GGYRCC 1 cut(s) 1027
BshVI ATCGAT 3 cut(s) 50, 135, 200
BsiHKCI CYCGRG 1 cut(s) 340
BsiSI CCGG 7 cut(s) 23, 168, 599, 1518, 1625, 1700, 1824
BslFI GGGAC 3 cut(s) 452, 905, 1827
BslI CCNNNNNNNGG 6 cut(s) 660, 929, 1160, 1396, 1827, 1830
BsmAI GTCTC 5 cut(s) 114, 320, 1429, 1688, 1720
BsmFI GGGAC 3 cut(s) 452, 905, 1827
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 6 cut(s) 150, 1108, 1153, 1205, 1579, 1687
Bso31I GGTCTC 1 cut(s) 320
BsoBI CYCGRG 1 cut(s) 340
Bsp120I GGGCCC 1 cut(s) 1106
Bsp1286I GDGCHC 4 cut(s) 1030, 1110, 1390, 1524
Bsp13I TCCGGA 1 cut(s) 22
Bsp143I GATC 5 cut(s) 876, 1258, 1291, 1366, 1493
Bsp1720I GCTNAGC 1 cut(s) 2040
Bsp68I TCGCGA 2 cut(s) 1281, 2073
BspACI CCGC 6 cut(s) 721, 1063, 1441, 1821, 1889, 1993
BspANI GGCC 6 cut(s) 150, 1108, 1153, 1205, 1579, 1687
BspCNI CTCAG 5 cut(s) 25, 323, 445, 1474, 2032
BspDI ATCGAT 3 cut(s) 50, 135, 200
BspEI TCCGGA 1 cut(s) 22
BspFNI CGCG 2 cut(s) 1281, 2073
BspLI GGNNCC 8 cut(s) 15, 483, 867, 1029, 1107, 1108, 1387, 1629
BspMI ACCTGC 2 cut(s) 493, 1053
BspPI GGATC 3 cut(s) 1253, 1374, 1501
BspT107I GGYRCC 1 cut(s) 1027
BspTI CTTAAG 1 cut(s) 8
BspTNI GGTCTC 1 cut(s) 320
BsrDI GCAATG 3 cut(s) 1028, 1084, 1945
BsrFI RCCGGY 1 cut(s) 598
BsrI ACTGG 9 cut(s) 301, 426, 1031, 1128, 1154, 1165, 1315, 1387, 1390
BssAI RCCGGY 1 cut(s) 598
BssECI CCNNGG 8 cut(s) 922, 923, 1016, 1051, 1574, 1612, 1824, 1878
BssMI GATC 5 cut(s) 876, 1258, 1291, 1366, 1493
BssNI GRCGYC 1 cut(s) 2068
BssSI CACGAG 1 cut(s) 152
BssT1I CCWWGG 2 cut(s) 1016, 1051
Bst2BI CACGAG 1 cut(s) 152
Bst2UI CCWGG 3 cut(s) 924, 1500, 1614
Bst6I CTCTTC 4 cut(s) 323, 597, 1433, 1797
BstACI GRCGYC 1 cut(s) 2068
BstAFI CTTAAG 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 1581
BstDEI CTNAG 6 cut(s) 33, 310, 372, 432, 1482, 2040
BstDSI CCRYGG 1 cut(s) 1574
BstF5I GGATG 5 cut(s) 180, 1477, 1495, 1632, 2025
BstFNI CGCG 2 cut(s) 1281, 2073
BstHHI GCGC 1 cut(s) 225
BstKTI GATC 5 cut(s) 879, 1261, 1294, 1369, 1496
BstMAI GTCTC 5 cut(s) 114, 320, 1429, 1688, 1720
BstMBI GATC 5 cut(s) 876, 1258, 1291, 1366, 1493
BstMWI GCNNNNNNNGC 6 cut(s) 11, 26, 38, 494, 1018, 1191
BstNI CCWGG 3 cut(s) 924, 1500, 1614
BstSCI CCNGG 7 cut(s) 166, 922, 1498, 1517, 1612, 1699, 1823
BstSFI CTRYAG 1 cut(s) 1088
BstSLI GKGCMC 2 cut(s) 1030, 1110
BstUI CGCG 2 cut(s) 1281, 2073
BstV1I GCAGC 6 cut(s) 16, 374, 475, 589, 730, 999
BstX2I RGATCY 1 cut(s) 1366
BstXI CCANNNNNNTGG 2 cut(s) 477, 1023
BstYI RGATCY 1 cut(s) 1366
Bsu15I ATCGAT 3 cut(s) 50, 135, 200
Bsu36I CCTNAGG 1 cut(s) 310
BsuI GTATCC 2 cut(s) 56, 82
BsuRI GGCC 6 cut(s) 150, 1108, 1153, 1205, 1579, 1687
BsuTUI ATCGAT 3 cut(s) 50, 135, 200
BtgI CCRYGG 1 cut(s) 1574
BtsCI GGATG 5 cut(s) 180, 1477, 1495, 1632, 2025
BtsI GCAGTG 2 cut(s) 114, 1341
BtsIMutI CAGTG 6 cut(s) 75, 114, 419, 1161, 1322, 1341
BtuMI TCGCGA 2 cut(s) 1281, 2073
BveI ACCTGC 2 cut(s) 493, 1053
Cac8I GCNNGC 1 cut(s) 1581
CaiI CAGNNNCTG 1 cut(s) 1160
CfoI GCGC 1 cut(s) 225
Cfr10I RCCGGY 1 cut(s) 598
Cfr13I GGNCC 6 cut(s) 237, 1055, 1106, 1107, 1619, 1685
ClaI ATCGAT 3 cut(s) 50, 135, 200
CseI GACGC 1 cut(s) 2057
Csp6I GTAC 2 cut(s) 94, 440
CspCI CAANNNNNGTGG 2 cut(s) 1713, 1748
CviAII CATG 3 cut(s) 944, 1334, 2089
CviQI GTAC 2 cut(s) 94, 440
DdeI CTNAG 6 cut(s) 33, 310, 372, 432, 1482, 2040
DpnI GATC 5 cut(s) 878, 1260, 1293, 1368, 1495
DpnII GATC 5 cut(s) 876, 1258, 1291, 1366, 1493
DriI GACNNNNNGTC 1 cut(s) 2067
EaeI YGGCCR 2 cut(s) 1203, 1577
Eam1104I CTCTTC 4 cut(s) 323, 597, 1433, 1797
Eam1105I GACNNNNNGTC 1 cut(s) 2067
EarI CTCTTC 4 cut(s) 323, 597, 1433, 1797
Eco130I CCWWGG 2 cut(s) 1016, 1051
Eco24I GRGCYC 3 cut(s) 1110, 1390, 1524
Eco31I GGTCTC 1 cut(s) 320
Eco32I GATATC 1 cut(s) 1752
Eco47I GGWCC 3 cut(s) 237, 1055, 1619
Eco81I CCTNAGG 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 340
EcoO109I RGGNCCY 2 cut(s) 1106, 1619
EcoRII CCWGG 3 cut(s) 922, 1498, 1612
EcoRV GATATC 1 cut(s) 1752
EcoT14I CCWWGG 2 cut(s) 1016, 1051
EcoT38I GRGCYC 3 cut(s) 1110, 1390, 1524
ErhI CCWWGG 2 cut(s) 1016, 1051
FaeI CATG 3 cut(s) 947, 1337, 2092
FaqI GGGAC 3 cut(s) 452, 905, 1827
FatI CATG 3 cut(s) 943, 1333, 2088
FbaI TGATCA 1 cut(s) 876
Fnu4HI GCNGC 6 cut(s) 30, 363, 489, 578, 719, 1013
FokI GGATG 5 cut(s) 167, 1464, 1502, 1619, 2032
FriOI GRGCYC 3 cut(s) 1110, 1390, 1524
Fsp4HI GCNGC 6 cut(s) 30, 363, 489, 578, 719, 1013
FspBI CTAG 3 cut(s) 741, 2057, 2064
GlaI GCGC 1 cut(s) 224
GluI GCNGC 6 cut(s) 30, 363, 489, 578, 719, 1013
GsuI CTGGAG 2 cut(s) 1404, 1635
HaeIII GGCC 6 cut(s) 150, 1108, 1153, 1205, 1579, 1687
HapII CCGG 7 cut(s) 23, 168, 599, 1518, 1625, 1700, 1824
HgaI GACGC 1 cut(s) 2057
HhaI GCGC 1 cut(s) 225
Hin1I GRCGYC 1 cut(s) 2068
Hin1II CATG 3 cut(s) 947, 1337, 2092
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HincII GTYRAC 4 cut(s) 294, 588, 1123, 1663
HindII GTYRAC 4 cut(s) 294, 588, 1123, 1663
HindIII AAGCTT 1 cut(s) 1646
HinfI GANTC 8 cut(s) 107, 231, 918, 933, 1283, 1355, 1437, 1696
HpaII CCGG 7 cut(s) 23, 168, 599, 1518, 1625, 1700, 1824
HphI GGTGA 3 cut(s) 1216, 1488, 1906
Hpy166II GTNNAC 7 cut(s) 294, 588, 1058, 1123, 1591, 1609, 1663
Hpy188III TCNNGA 9 cut(s) 23, 281, 937, 1280, 1432, 1481, 2034, 2072, 2097
Hpy8I GTNNAC 7 cut(s) 294, 588, 1058, 1123, 1591, 1609, 1663
Hpy99I CGWCG 3 cut(s) 245, 1281, 2073
HpyAV CCTTC 4 cut(s) 684, 1252, 1735, 1967
HpyCH4IV ACGT 2 cut(s) 243, 1119
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 26, 38, 494, 1018, 1191
HpyF3I CTNAG 6 cut(s) 33, 310, 372, 432, 1482, 2040
HpySE526I ACGT 2 cut(s) 243, 1119
Hsp92I GRCGYC 1 cut(s) 2068
Hsp92II CATG 3 cut(s) 947, 1337, 2092
HspAI GCGC 1 cut(s) 223
Kpn2I TCCGGA 1 cut(s) 22
Ksp22I TGATCA 1 cut(s) 876
Kzo9I GATC 5 cut(s) 876, 1258, 1291, 1366, 1493
LmnI GCTCC 6 cut(s) 19, 25, 715, 865, 1385, 1576
Lsp1109I GCAGC 6 cut(s) 16, 374, 475, 589, 730, 999
LweI GCATC 4 cut(s) 56, 349, 640, 1486
MaeI CTAG 3 cut(s) 741, 2057, 2064
MaeII ACGT 2 cut(s) 243, 1119
MaeIII GTNAC 6 cut(s) 686, 846, 1040, 1156, 1378, 1392
MalI GATC 5 cut(s) 878, 1260, 1293, 1368, 1495
MboI GATC 5 cut(s) 876, 1258, 1291, 1366, 1493
MfeI CAATTG 2 cut(s) 42, 978
MflI RGATCY 1 cut(s) 1366
MhlI GDGCHC 4 cut(s) 1030, 1110, 1390, 1524
MlsI TGGCCA 1 cut(s) 1205
MluCI AATT 7 cut(s) 42, 322, 897, 978, 1405, 1655, 1997
MluNI TGGCCA 1 cut(s) 1205
MlyI GAGTC 5 cut(s) 116, 927, 1364, 1431, 1690
MmeI TCCRAC 5 cut(s) 264, 280, 843, 1270, 1788
Mox20I TGGCCA 1 cut(s) 1205
MroI TCCGGA 1 cut(s) 22
MscI TGGCCA 1 cut(s) 1205
MseI TTAA 7 cut(s) 9, 83, 204, 459, 725, 788, 1100
MslI CAYNNNNRTG 1 cut(s) 475
Msp20I TGGCCA 1 cut(s) 1205
MspA1I CMGCKG 3 cut(s) 721, 1012, 1063
MspCI CTTAAG 1 cut(s) 8
MspI CCGG 7 cut(s) 23, 168, 599, 1518, 1625, 1700, 1824
MspR9I CCNGG 7 cut(s) 168, 924, 1500, 1519, 1614, 1701, 1825
MunI CAATTG 2 cut(s) 42, 978
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 3 cut(s) 924, 1500, 1614
MvnI CGCG 2 cut(s) 1281, 2073
MwoI GCNNNNNNNGC 6 cut(s) 11, 26, 38, 494, 1018, 1191
NciI CCSGG 4 cut(s) 168, 1519, 1701, 1825
NdeII GATC 5 cut(s) 876, 1258, 1291, 1366, 1493
NlaIII CATG 3 cut(s) 947, 1337, 2092
NlaIV GGNNCC 8 cut(s) 15, 483, 867, 1029, 1107, 1108, 1387, 1629
NmeAIII GCCGAG 1 cut(s) 1903
NmuCI GTSAC 2 cut(s) 1040, 1156
NruI TCGCGA 2 cut(s) 1281, 2073
PasI CCCWGGG 1 cut(s) 923
PctI GAATGC 1 cut(s) 580
PfeI GAWTC 3 cut(s) 231, 933, 1283
PflMI CCANNNNNTGG 1 cut(s) 1160
PkrI GCNGC 6 cut(s) 31, 364, 490, 579, 720, 1014
PleI GAGTC 5 cut(s) 115, 926, 1363, 1431, 1690
PpsI GAGTC 5 cut(s) 115, 926, 1363, 1431, 1690
PpuMI RGGWCCY 1 cut(s) 1619
Psp5II RGGWCCY 1 cut(s) 1619
Psp6I CCWGG 3 cut(s) 922, 1498, 1612
PspGI CCWGG 3 cut(s) 922, 1498, 1612
PspN4I GGNNCC 8 cut(s) 15, 483, 867, 1029, 1107, 1108, 1387, 1629
PspOMI GGGCCC 1 cut(s) 1106
PspPI GGNCC 6 cut(s) 237, 1055, 1106, 1107, 1619, 1685
PspPPI RGGWCCY 1 cut(s) 1619
PstNI CAGNNNCTG 1 cut(s) 1160
PsuI RGATCY 1 cut(s) 1366
PvuII CAGCTG 1 cut(s) 1012
RruI TCGCGA 2 cut(s) 1281, 2073
RsaI GTAC 2 cut(s) 95, 441
RsaNI GTAC 2 cut(s) 94, 440
RseI CAYNNNNRTG 1 cut(s) 475
SaqAI TTAA 7 cut(s) 9, 83, 204, 459, 725, 788, 1100
SatI GCNGC 6 cut(s) 30, 363, 489, 578, 719, 1013
Sau3AI GATC 5 cut(s) 876, 1258, 1291, 1366, 1493
Sau96I GGNCC 6 cut(s) 237, 1055, 1106, 1107, 1619, 1685
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 5 cut(s) 116, 927, 1364, 1431, 1690
ScrFI CCNGG 7 cut(s) 168, 924, 1500, 1519, 1614, 1701, 1825
SduI GDGCHC 4 cut(s) 1030, 1110, 1390, 1524
SfaNI GCATC 4 cut(s) 56, 349, 640, 1486
SfcI CTRYAG 1 cut(s) 1088
SinI GGWCC 3 cut(s) 237, 1055, 1619
SmiMI CAYNNNNRTG 1 cut(s) 475
SmlI CTYRAG 4 cut(s) 8, 89, 937, 1432
SmoI CTYRAG 4 cut(s) 8, 89, 937, 1432
SpeI ACTAGT 1 cut(s) 740
Sse9I AATT 7 cut(s) 42, 322, 897, 978, 1405, 1655, 1997
SsiI CCGC 6 cut(s) 721, 1063, 1441, 1821, 1889, 1993
SspI AATATT 2 cut(s) 190, 1788
SspMI CTAG 3 cut(s) 741, 2057, 2064
StyD4I CCNGG 7 cut(s) 166, 922, 1498, 1517, 1612, 1699, 1823
StyI CCWWGG 2 cut(s) 1016, 1051
TaiI ACGT 2 cut(s) 246, 1122
TaqI TCGA 6 cut(s) 50, 121, 135, 200, 1251, 1860
TasI AATT 7 cut(s) 42, 322, 897, 978, 1405, 1655, 1997
TatI WGTACW 1 cut(s) 93
TfiI GAWTC 3 cut(s) 231, 933, 1283
Tru1I TTAA 7 cut(s) 9, 83, 204, 459, 725, 788, 1100
Tru9I TTAA 7 cut(s) 9, 83, 204, 459, 725, 788, 1100
TscAI CASTG 6 cut(s) 82, 121, 426, 1161, 1322, 1348
TseFI GTSAC 2 cut(s) 1040, 1156
TseI GCWGC 6 cut(s) 29, 362, 488, 577, 718, 1012
Tsp45I GTSAC 2 cut(s) 1040, 1156
TspDTI ATGAA 4 cut(s) 388, 584, 855, 1896
TspGWI ACGGA 2 cut(s) 1685, 1748
TspRI CASTG 6 cut(s) 82, 121, 426, 1161, 1322, 1348
Van91I CCANNNNNTGG 1 cut(s) 1160
Vha464I CTTAAG 1 cut(s) 8
VpaK11BI GGWCC 3 cut(s) 237, 1055, 1619
XapI RAATTY 1 cut(s) 1655
XspI CTAG 3 cut(s) 741, 2057, 2064
ZrmI AGTACT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.