pycom16g00670
NAC Family

Inactive leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012108
Physical Location & Seq
Reverse (-)
404258 .. 408293
4036 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2004 bp
ATGGTTTCTCAAAAAGGTGAAATCTTTTTCTTATTGCTGCCATTGCTGTTCTGGGTTTTTCTGGGTTCGGCAAAAGCAGGGGATGCGGATGCTCTGTTGACTCTGAAATCTACAATCGACCCTCAAAACGCGCTGCCTTGGCGAGAGGGAAGCAACGTGTGCGAGTGGGAAGGCGTGAAGGAATGCCTGAGAGGGAGAGTTACGAAGCTTGTGCTGGAGTATTCGAACCTCACCGGAGTTCTGGATCCCAAGATCTTGAACCGGCTGGACCAGCTCCGAGTCCTCAGCTTCAAAGGCAACTCCCTCTCCGGCCAAATCCCCGACCTCTCAGGCCTCCTCAACCTCAAATCCCTCTTCCTAAACGACAACAACTTCTCCGGCGTTTTCCCCTCTTCCATTTCCGACCTCCACCGCCTCAAAGTCGTCGTCCTCGCCGGAAACAAAATCTCCGGCGAAATCCCAATGTCGCTGCTCAGGCTCCGCCGCCTCTACGTCCTGTACTTGCAGGACAACAGGTTCACCGGTCCCATTCCGCCCCTCAACCAGACCTCCCTCCGGTTCTTCAACGTGTCGAATAACCAGCTATCCGGGGAAATTCCGGTAACCCCACCCTTGATTCTGTTTAATGCGTCGTCGTTTTTAGGGAACGTCGGTGTCTGCGGGGTGCAAATTGACAAACCGTGCGGGGGCATTATCGGGTTTCCACCGTCGATCAGTCCCACCAGTCAACCGGGTCCAAAGTCAAAGTCCAAGCGCGGGAAACTGATAAAGATAATCGCAGGGAGCGTGGGCGGGTTCATGGTGGTCGTAATCTGCTTAGTTGTGCTGTGGATGGTTTGCCGGAGGAGTGGCGGCGGTGGTGGGGAGGTAAGCAGGAGCAAAGGCGGGGTTGTTGGGGGTGCTGAGCCGACAACAGAGAGAGGAGGAGGATCAGGGGAGGGGCCCGCAGGTCCGCCCAGTGGCAGTGATGGTAATAATGGTGGGAAACAAGGGGGTTTTTCGTGGGAAGGTGAGGGGCTGGGGAGTTTGGTGTTCTGCGGAGCAGGGGATCAGCAGATGAGTTACAGCCTGGAGGATCTGCTGAAGGCGTCGGCGGAGACGCTGGGGAGGGGCACGTTGGGAAGTACGTACAAGGCGGTGATGGAATCCGGGTTTATCGTGACGGTGAAGCGGCTGAAGGACGCGAGGTACCCGAGGATGGAGGAGTTTAGGAGACACGTGGACTTGCTAGGGCAGCTGAGGCACCCACACTTGGTCCCACTGAGGGCGTACTTCCAGGCTAAGGAGGAACGCCTGCTCGTATACGATTATTTCCCCAACGGCAGCCTCTTTTCACTTGTTCATGGTTCAAGAACTTCAGGAGGCGGTAAGCCGCTTCACTGGACGTCATGTCTTAAAATAGCTGAGGACTTGGCGAGTGGAGTTGTTTATATCCACCAGAACCCCGGCTTAACTCATGGAAACTTGAAATCCTCAAACGTCTTGTTAGGTTCCGATTTTGAATCCTGCTTGACGGATTATGGTCTCACATTATTCGGAGATCCTAATTCACTCGAAGAACCTAGCGCAACCACTCTCTTTTATAGAGCACCAGAATGTCGCGACATCAGAAAACCATCAACCCAACCAGCTGATGTGTATAGCTTCGGCGTCCTCCTCTTGGAGCTCCTAACCGGAAAGACTCCCTTCCAAGACCTTGTCCAGGAACATGGTTCGGACATCCCTAGCTGGGTCCGCTCAGTGCGCGAAGAAGAGACTGAGTCAGGGGATGACCCTGTCTCGGGCAATGAGGCATCAGAGGAGAAGCTGCAGGCACTTCTTAACATTGCAATGGCTTGTGTTTCGCTTTCACCGGAGAACCGACCGGCGATGAGAGATGTTCTAAGGATGATAAGGGATGCTAGGGCGGAGGGCCAAGTGTCTTCCAACAGCAGTGATCACTCGCCGGGGAGATGGTCGGATACCGTTCAGAGTCTGCCAAGGGAAGAACACTTGAGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

668

Amino Acids

72.16

Weight (kDa)

6.63

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 86 - 171 9.1e-07 Leucine-rich repeat region
LRR_8 PF13855 114 - 173 8.2e-08 Leucine rich repeat
Pkinase PF00069 365 - 629 1.2e-33 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 366 - 629 1.4e-30 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10850
fragaria_vesca FvH4_4g36460
malus_domestica MD13G1009100.v1.1 MD16G1007100.v1.1
prunus_persica Prupe.1G343600_v2.0.a1
pyrus_communis pycom13g00770 pycom16g00670
rosa_chinensis RchiOBHm_Chr4g0446221
rosa_laevigata RLG00000005680
rosa_multiflora Rmu_co8398623.1_g000001 Rmu_sc0002266.1_g000012 Rmu_sc0040593.1_g000001
rosa_roxburghii Rroxscaffold_5G00386620
rosa_rugosa Rorug04G0368500
rosa_samantha Rh4AG430700 Rh4BG440100 Rh4CG457400 Rh4DG438600
rosa_wichuraiana Rw4G036790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1388
Acc36I ACCTGC 1 cut(s) 938
Acc65I GGTACC 1 cut(s) 1188
AccB1I GGYRCC 2 cut(s) 1188, 1242
AccBSI CCGCTC 1 cut(s) 1737
AccI GTMKAC 1 cut(s) 1302
AccII CGCG 5 cut(s) 131, 756, 1184, 1602, 1746
AclWI GGATC 6 cut(s) 239, 252, 937, 1056, 1083, 1535
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 1 cut(s) 594
AcuI CTGAAG 3 cut(s) 1103, 1196, 1341
AcvI CACGTG 1 cut(s) 1219
AcyI GRCGYC 3 cut(s) 1088, 1385, 1650
AfaI GTAC 5 cut(s) 500, 1126, 1130, 1190, 1271
AflIII ACRYGT 3 cut(s) 156, 567, 1216
AgeI ACCGGT 1 cut(s) 521
AgsI TTSAA 6 cut(s) 259, 292, 565, 1350, 1468, 1502
AhdI GACNNNNNGTC 1 cut(s) 1389
AjnI CCWGG 3 cut(s) 1068, 1275, 1701
AloI GAACNNNNNNTCC 2 cut(s) 500, 532
Alw21I GWGCWC 2 cut(s) 1591, 1668
Alw26I GTCTC 5 cut(s) 1091, 1207, 1529, 1748, 1783
AlwI GGATC 6 cut(s) 239, 252, 937, 1056, 1083, 1535
AlwNI CAGNNNCTG 1 cut(s) 1975
Ama87I CYCGRG 2 cut(s) 1192, 1780
AoxI GGCC 4 cut(s) 310, 331, 941, 1912
ApaI GGGCCC 1 cut(s) 945
ApeKI GCWGC 6 cut(s) 37, 133, 469, 1234, 1323, 1807
ApoI RAATTY 1 cut(s) 594
ArsI GACNNNNNNTTYG 4 cut(s) 411, 443, 731, 763
AsiGI ACCGGT 1 cut(s) 521
Asp718I GGTACC 1 cut(s) 1188
AspLEI GCGC 4 cut(s) 133, 756, 1568, 1746
AspS9I GGNCC 9 cut(s) 268, 524, 734, 941, 942, 950, 1255, 1732, 1912
AsuC2I CCSGG 5 cut(s) 589, 732, 1150, 1446, 1947
AsuHPI GGTGA 7 cut(s) 29, 223, 511, 1022, 1150, 1177, 1842
AsuII TTCGAA 1 cut(s) 224
AvaI CYCGRG 2 cut(s) 1192, 1780
AvaII GGWCC 6 cut(s) 268, 524, 734, 950, 1255, 1732
BaeGI GKGCMC 2 cut(s) 945, 1115
BamHI GGATCC 1 cut(s) 244
BanI GGYRCC 2 cut(s) 1188, 1242
BanII GRGCYC 2 cut(s) 945, 1668
BbrPI CACGTG 1 cut(s) 1219
BbsI GAAGAC 1 cut(s) 1914
Bbv12I GWGCWC 2 cut(s) 1591, 1668
BbvCI CCTCAGC 3 cut(s) 284, 1238, 1404
BbvI GCAGC 6 cut(s) 24, 120, 456, 1246, 1335, 1794
BccI CCATC 6 cut(s) 826, 962, 1135, 1192, 1624, 1947
BceAI ACGGC 1 cut(s) 1336
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 3 cut(s) 1070, 1277, 1703
BciVI GTATCC 1 cut(s) 1954
BclI TGATCA 1 cut(s) 1936
BcnI CCSGG 5 cut(s) 589, 732, 1150, 1446, 1947
BcoDI GTCTC 5 cut(s) 1091, 1207, 1529, 1748, 1783
BfaI CTAG 4 cut(s) 1229, 1563, 1725, 1902
BfmI CTRYAG 1 cut(s) 1808
BfuAI ACCTGC 1 cut(s) 938
BfuI GTATCC 1 cut(s) 1954
BglII AGATCT 1 cut(s) 252
BlpI GCTNAGC 1 cut(s) 903
Bme1390I CCNGG 8 cut(s) 589, 732, 1070, 1150, 1277, 1446, 1703, 1947
Bme18I GGWCC 6 cut(s) 268, 524, 734, 950, 1255, 1732
BmeRI GACNNNNNGTC 1 cut(s) 1389
BmeT110I CYCGRG 2 cut(s) 1192, 1780
BmgT120I GGNCC 9 cut(s) 268, 524, 734, 941, 942, 950, 1255, 1732, 1912
BmrFI CCNGG 8 cut(s) 589, 732, 1070, 1150, 1277, 1446, 1703, 1947
BmrI ACTGGG 1 cut(s) 951
BmsI GCATC 4 cut(s) 73, 79, 1802, 1888
BmuI ACTGGG 1 cut(s) 951
BpiI GAAGAC 1 cut(s) 1914
BpmI CTGGAG 2 cut(s) 236, 1091
Bpu10I CCTNAGC 5 cut(s) 284, 473, 1238, 1281, 1404
Bpu1102I GCTNAGC 1 cut(s) 903
Bpu14I TTCGAA 1 cut(s) 224
BpuMI CCSGG 5 cut(s) 589, 732, 1150, 1446, 1947
BsaAI YACGTR 2 cut(s) 1128, 1219
BsaHI GRCGYC 3 cut(s) 1088, 1385, 1650
BsaI GGTCTC 1 cut(s) 1529
BsaJI CCNNGG 6 cut(s) 137, 588, 1193, 1444, 1946, 1979
BsaWI WCCGGW 6 cut(s) 233, 521, 555, 598, 1673, 1852
BsaXI ACNNNNNCTCC 8 cut(s) 290, 320, 359, 389, 431, 461, 533, 563
Bse118I RCCGGY 3 cut(s) 261, 521, 1864
Bse1I ACTGG 3 cut(s) 723, 957, 1385
Bse3DI GCAATG 4 cut(s) 41, 1792, 1824, 1836
BseBI CCWGG 3 cut(s) 1070, 1277, 1703
BseDI CCNNGG 6 cut(s) 137, 588, 1193, 1444, 1946, 1979
BseGI GGATG 8 cut(s) 88, 94, 837, 1203, 1719, 1774, 1893, 1903
BseMI GCAATG 4 cut(s) 41, 1792, 1824, 1836
BseNI ACTGG 3 cut(s) 723, 957, 1385
BseRI GAGGAG 7 cut(s) 326, 859, 936, 939, 1217, 1646, 1814
BseSI GKGCMC 2 cut(s) 945, 1115
BseXI GCAGC 6 cut(s) 24, 120, 456, 1246, 1335, 1794
BseYI CCCAGC 3 cut(s) 1018, 1102, 1728
Bsh1236I CGCG 5 cut(s) 131, 756, 1184, 1602, 1746
Bsh1285I CGRYCG 1 cut(s) 1865
BshFI GGCC 4 cut(s) 312, 333, 943, 1914
BshNI GGYRCC 2 cut(s) 1188, 1242
BshTI ACCGGT 1 cut(s) 521
BsiEI CGRYCG 1 cut(s) 1865
BsiHKAI GWGCWC 2 cut(s) 1591, 1668
BsiHKCI CYCGRG 2 cut(s) 1192, 1780
BslFI GGGAC 3 cut(s) 510, 702, 1241
BsmAI GTCTC 5 cut(s) 1091, 1207, 1529, 1748, 1783
BsmBI CGTCTC 1 cut(s) 1091
BsmFI GGGAC 3 cut(s) 510, 702, 1241
BsmI GAATGC 1 cut(s) 188
BsnI GGCC 4 cut(s) 312, 333, 943, 1914
Bso31I GGTCTC 1 cut(s) 1529
BsoBI CYCGRG 2 cut(s) 1192, 1780
Bsp119I TTCGAA 1 cut(s) 224
Bsp120I GGGCCC 1 cut(s) 941
Bsp1286I GDGCHC 4 cut(s) 945, 1115, 1591, 1668
Bsp143I GATC 8 cut(s) 244, 252, 711, 929, 1048, 1075, 1540, 1936
Bsp1720I GCTNAGC 1 cut(s) 903
Bsp68I TCGCGA 1 cut(s) 1602
BspANI GGCC 4 cut(s) 312, 333, 943, 1914
BspFNI CGCG 5 cut(s) 131, 756, 1184, 1602, 1746
BspMAI CTGCAG 1 cut(s) 1812
BspMI ACCTGC 1 cut(s) 938
BspPI GGATC 6 cut(s) 239, 252, 937, 1056, 1083, 1535
BspT104I TTCGAA 1 cut(s) 224
BspT107I GGYRCC 2 cut(s) 1188, 1242
BspTNI GGTCTC 1 cut(s) 1529
BsrBI CCGCTC 1 cut(s) 1737
BsrDI GCAATG 4 cut(s) 41, 1792, 1824, 1836
BsrFI RCCGGY 3 cut(s) 261, 521, 1864
BsrI ACTGG 3 cut(s) 723, 957, 1385
BssAI RCCGGY 3 cut(s) 261, 521, 1864
BssECI CCNNGG 6 cut(s) 137, 588, 1193, 1444, 1946, 1979
BssMI GATC 8 cut(s) 244, 252, 711, 929, 1048, 1075, 1540, 1936
BssNAI GTATAC 1 cut(s) 1303
BssNI GRCGYC 3 cut(s) 1088, 1385, 1650
BssT1I CCWWGG 2 cut(s) 137, 1979
Bst1107I GTATAC 1 cut(s) 1303
Bst2UI CCWGG 3 cut(s) 1070, 1277, 1703
Bst4CI ACNGT 4 cut(s) 681, 708, 1165, 1966
Bst6I CTCTTC 3 cut(s) 359, 397, 1746
BstACI GRCGYC 3 cut(s) 1088, 1385, 1650
BstAPI GCANNNNNTGC 2 cut(s) 83, 159
BstBAI YACGTR 2 cut(s) 1128, 1219
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 3 cut(s) 945, 1295, 1812
BstEII GGTNACC 1 cut(s) 601
BstENI CCTNNNNNAGG 1 cut(s) 1700
BstF5I GGATG 8 cut(s) 88, 94, 837, 1203, 1719, 1774, 1893, 1903
BstFNI CGCG 5 cut(s) 131, 756, 1184, 1602, 1746
BstHHI GCGC 4 cut(s) 133, 756, 1568, 1746
BstKTI GATC 8 cut(s) 247, 255, 714, 932, 1051, 1078, 1543, 1939
BstMAI GTCTC 5 cut(s) 1091, 1207, 1529, 1748, 1783
BstMBI GATC 8 cut(s) 244, 252, 711, 929, 1048, 1075, 1540, 1936
BstMCI CGRYCG 1 cut(s) 1865
BstNI CCWGG 3 cut(s) 1070, 1277, 1703
BstPI GGTNACC 1 cut(s) 601
BstSCI CCNGG 8 cut(s) 587, 730, 1068, 1148, 1275, 1444, 1701, 1945
BstSFI CTRYAG 1 cut(s) 1808
BstSLI GKGCMC 2 cut(s) 945, 1115
BstSNI TACGTA 1 cut(s) 1128
BstUI CGCG 5 cut(s) 131, 756, 1184, 1602, 1746
BstV1I GCAGC 6 cut(s) 24, 120, 456, 1246, 1335, 1794
BstV2I GAAGAC 1 cut(s) 1914
BstX2I RGATCY 4 cut(s) 244, 252, 1075, 1540
BstXI CCANNNNNNTGG 1 cut(s) 1709
BstYI RGATCY 4 cut(s) 244, 252, 1075, 1540
BstZ17I GTATAC 1 cut(s) 1303
BsuI GTATCC 1 cut(s) 1954
BsuRI GGCC 4 cut(s) 312, 333, 943, 1914
BtgZI GCGATG 1 cut(s) 1883
BtsCI GGATG 8 cut(s) 88, 94, 837, 1203, 1719, 1774, 1893, 1903
BtsI GCAGTG 2 cut(s) 970, 1939
BtsIMutI CAGTG 6 cut(s) 964, 970, 1259, 1378, 1746, 1939
BtuMI TCGCGA 1 cut(s) 1602
BveI ACCTGC 1 cut(s) 938
Cac8I GCNNGC 3 cut(s) 945, 1295, 1812
CaiI CAGNNNCTG 1 cut(s) 1975
CfoI GCGC 4 cut(s) 133, 756, 1568, 1746
Cfr10I RCCGGY 3 cut(s) 261, 521, 1864
Cfr13I GGNCC 9 cut(s) 268, 524, 734, 941, 942, 950, 1255, 1732, 1912
CseI GACGC 5 cut(s) 618, 1077, 1108, 1190, 1639
Csp6I GTAC 5 cut(s) 499, 1125, 1129, 1189, 1270
CspAI ACCGGT 1 cut(s) 521
CviAII CATG 5 cut(s) 799, 1343, 1389, 1457, 1709
CviQI GTAC 5 cut(s) 499, 1125, 1129, 1189, 1270
DpnI GATC 8 cut(s) 246, 254, 713, 931, 1050, 1077, 1542, 1938
DpnII GATC 8 cut(s) 244, 252, 711, 929, 1048, 1075, 1540, 1936
DriI GACNNNNNGTC 1 cut(s) 1389
EaeI YGGCCR 1 cut(s) 310
Eam1104I CTCTTC 3 cut(s) 359, 397, 1746
Eam1105I GACNNNNNGTC 1 cut(s) 1389
EarI CTCTTC 3 cut(s) 359, 397, 1746
EciI GGCGGA 5 cut(s) 470, 522, 942, 1109, 1922
Ecl136II GAGCTC 1 cut(s) 1666
Eco105I TACGTA 1 cut(s) 1128
Eco130I CCWWGG 2 cut(s) 137, 1979
Eco147I AGGCCT 1 cut(s) 333
Eco24I GRGCYC 2 cut(s) 945, 1668
Eco31I GGTCTC 1 cut(s) 1529
Eco47I GGWCC 6 cut(s) 268, 524, 734, 950, 1255, 1732
Eco53kI GAGCTC 1 cut(s) 1666
Eco57I CTGAAG 3 cut(s) 1103, 1196, 1341
Eco72I CACGTG 1 cut(s) 1219
Eco88I CYCGRG 2 cut(s) 1192, 1780
Eco91I GGTNACC 1 cut(s) 601
EcoICRI GAGCTC 1 cut(s) 1666
EcoNI CCTNNNNNAGG 1 cut(s) 1700
EcoO109I RGGNCCY 1 cut(s) 941
EcoO65I GGTNACC 1 cut(s) 601
EcoRII CCWGG 3 cut(s) 1068, 1275, 1701
EcoT14I CCWWGG 2 cut(s) 137, 1979
EcoT38I GRGCYC 2 cut(s) 945, 1668
ErhI CCWWGG 2 cut(s) 137, 1979
Esp3I CGTCTC 1 cut(s) 1091
FaeI CATG 5 cut(s) 802, 1346, 1392, 1460, 1712
FalI AAGNNNNNCTT 2 cut(s) 1670, 1702
FaqI GGGAC 3 cut(s) 510, 702, 1241
FatI CATG 5 cut(s) 798, 1342, 1388, 1456, 1708
FauI CCCGC 6 cut(s) 653, 677, 749, 785, 878, 952
FbaI TGATCA 1 cut(s) 1936
FblI GTMKAC 1 cut(s) 1302
FokI GGATG 8 cut(s) 95, 101, 844, 1210, 1706, 1781, 1900, 1910
FriOI GRGCYC 2 cut(s) 945, 1668
FspBI CTAG 4 cut(s) 1229, 1563, 1725, 1902
GlaI GCGC 4 cut(s) 132, 755, 1567, 1745
GsaI CCCAGC 3 cut(s) 1022, 1106, 1732
GsuI CTGGAG 2 cut(s) 236, 1091
HaeIII GGCC 4 cut(s) 312, 333, 943, 1914
HgaI GACGC 5 cut(s) 618, 1077, 1108, 1190, 1639
HhaI GCGC 4 cut(s) 133, 756, 1568, 1746
Hin1I GRCGYC 3 cut(s) 1088, 1385, 1650
Hin1II CATG 5 cut(s) 802, 1346, 1392, 1460, 1712
Hin6I GCGC 4 cut(s) 131, 754, 1566, 1744
HinP1I GCGC 4 cut(s) 131, 754, 1566, 1744
HincII GTYRAC 2 cut(s) 99, 728
HindII GTYRAC 2 cut(s) 99, 728
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 8 cut(s) 100, 279, 616, 1145, 1502, 1681, 1760, 1972
HphI GGTGA 7 cut(s) 29, 223, 511, 1022, 1150, 1177, 1842
Hpy166II GTNNAC 5 cut(s) 99, 519, 728, 1222, 1303
Hpy188III TCNNGA 6 cut(s) 242, 256, 1159, 1350, 1359, 1601
Hpy8I GTNNAC 5 cut(s) 99, 519, 728, 1222, 1303
Hpy99I CGWCG 6 cut(s) 428, 634, 637, 653, 712, 1093
HpyAV CCTTC 6 cut(s) 164, 172, 1001, 1078, 1171, 1696
HpyCH4III ACNGT 4 cut(s) 681, 708, 1165, 1966
HpyCH4IV ACGT 9 cut(s) 156, 492, 567, 648, 1115, 1127, 1218, 1385, 1479
HpyCH4V TGCA 4 cut(s) 505, 667, 1810, 1829
HpySE526I ACGT 9 cut(s) 156, 492, 567, 648, 1115, 1127, 1218, 1385, 1479
Hsp92I GRCGYC 3 cut(s) 1088, 1385, 1650
Hsp92II CATG 5 cut(s) 802, 1346, 1392, 1460, 1712
HspAI GCGC 4 cut(s) 131, 754, 1566, 1744
KpnI GGTACC 1 cut(s) 1192
Ksp22I TGATCA 1 cut(s) 1936
Kzo9I GATC 8 cut(s) 244, 252, 711, 929, 1048, 1075, 1540, 1936
LmnI GCTCC 7 cut(s) 279, 483, 783, 876, 1040, 1663, 1671
Lsp1109I GCAGC 6 cut(s) 24, 120, 456, 1246, 1335, 1794
LweI GCATC 4 cut(s) 73, 79, 1802, 1888
MaeI CTAG 4 cut(s) 1229, 1563, 1725, 1902
MaeII ACGT 9 cut(s) 156, 492, 567, 648, 1115, 1127, 1218, 1385, 1479
MaeIII GTNAC 4 cut(s) 199, 601, 1061, 1159
MalI GATC 8 cut(s) 246, 254, 713, 931, 1050, 1077, 1542, 1938
MbiI CCGCTC 1 cut(s) 1737
MboI GATC 8 cut(s) 244, 252, 711, 929, 1048, 1075, 1540, 1936
MboII GAAGA 8 cut(s) 346, 384, 553, 1568, 1760, 1763, 1914, 1997
MflI RGATCY 4 cut(s) 244, 252, 1075, 1540
MhlI GDGCHC 4 cut(s) 945, 1115, 1591, 1668
MluCI AATT 3 cut(s) 594, 669, 1546
MlyI GAGTC 5 cut(s) 94, 288, 1675, 1769, 1981
MmeI TCCRAC 3 cut(s) 426, 1938, 1950
MseI TTAA 4 cut(s) 624, 1395, 1451, 1821
MslI CAYNNNNRTG 2 cut(s) 1594, 1829
MspA1I CMGCKG 2 cut(s) 1237, 1631
MspR9I CCNGG 8 cut(s) 589, 732, 1070, 1150, 1277, 1446, 1703, 1947
Mva1269I GAATGC 1 cut(s) 188
MvaI CCWGG 3 cut(s) 1070, 1277, 1703
MvnI CGCG 5 cut(s) 131, 756, 1184, 1602, 1746
NciI CCSGG 5 cut(s) 589, 732, 1150, 1446, 1947
NdeII GATC 8 cut(s) 244, 252, 711, 929, 1048, 1075, 1540, 1936
NlaIII CATG 5 cut(s) 802, 1346, 1392, 1460, 1712
NmuCI GTSAC 1 cut(s) 1159
NruI TCGCGA 1 cut(s) 1602
NspV TTCGAA 1 cut(s) 224
PceI AGGCCT 1 cut(s) 333
PcsI WCGNNNNNNNCGW 1 cut(s) 783
PctI GAATGC 1 cut(s) 188
PfeI GAWTC 3 cut(s) 616, 1145, 1502
PflFI GACNNNGTC 3 cut(s) 1697, 1759, 1775
PfoI TCCNGGA 1 cut(s) 1701
PinAI ACCGGT 1 cut(s) 521
PleI GAGTC 5 cut(s) 94, 287, 1675, 1768, 1980
PmaCI CACGTG 1 cut(s) 1219
PmlI CACGTG 1 cut(s) 1219
PpsI GAGTC 5 cut(s) 94, 287, 1675, 1768, 1980
Ppu21I YACGTR 2 cut(s) 1128, 1219
Psp124BI GAGCTC 1 cut(s) 1668
Psp6I CCWGG 3 cut(s) 1068, 1275, 1701
PspCI CACGTG 1 cut(s) 1219
PspEI GGTNACC 1 cut(s) 601
PspFI CCCAGC 3 cut(s) 1018, 1102, 1728
PspGI CCWGG 3 cut(s) 1068, 1275, 1701
PspOMI GGGCCC 1 cut(s) 941
PspPI GGNCC 9 cut(s) 268, 524, 734, 941, 942, 950, 1255, 1732, 1912
PstI CTGCAG 1 cut(s) 1812
PstNI CAGNNNCTG 1 cut(s) 1975
PsuI RGATCY 4 cut(s) 244, 252, 1075, 1540
PsyI GACNNNGTC 3 cut(s) 1697, 1759, 1775
PvuII CAGCTG 2 cut(s) 1237, 1631
RruI TCGCGA 1 cut(s) 1602
RsaI GTAC 5 cut(s) 500, 1126, 1130, 1190, 1271
RsaNI GTAC 5 cut(s) 499, 1125, 1129, 1189, 1270
RseI CAYNNNNRTG 2 cut(s) 1594, 1829
SacI GAGCTC 1 cut(s) 1668
SaqAI TTAA 4 cut(s) 624, 1395, 1451, 1821
Sau3AI GATC 8 cut(s) 244, 252, 711, 929, 1048, 1075, 1540, 1936
Sau96I GGNCC 9 cut(s) 268, 524, 734, 941, 942, 950, 1255, 1732, 1912
SchI GAGTC 5 cut(s) 94, 288, 1675, 1769, 1981
ScrFI CCNGG 8 cut(s) 589, 732, 1070, 1150, 1277, 1446, 1703, 1947
SduI GDGCHC 4 cut(s) 945, 1115, 1591, 1668
SfaNI GCATC 4 cut(s) 73, 79, 1802, 1888
SfcI CTRYAG 1 cut(s) 1808
SfuI TTCGAA 1 cut(s) 224
SinI GGWCC 6 cut(s) 268, 524, 734, 950, 1255, 1732
SmiMI CAYNNNNRTG 2 cut(s) 1594, 1829
SmlI CTYRAG 1 cut(s) 1993
SmoI CTYRAG 1 cut(s) 1993
SnaBI TACGTA 1 cut(s) 1128
Sse9I AATT 3 cut(s) 594, 669, 1546
SseBI AGGCCT 1 cut(s) 333
SspMI CTAG 4 cut(s) 1229, 1563, 1725, 1902
SstI GAGCTC 1 cut(s) 1668
StuI AGGCCT 1 cut(s) 333
StyD4I CCNGG 8 cut(s) 587, 730, 1068, 1148, 1275, 1444, 1701, 1945
StyI CCWWGG 2 cut(s) 137, 1979
TaaI ACNGT 4 cut(s) 681, 708, 1165, 1966
TaiI ACGT 9 cut(s) 159, 495, 570, 651, 1118, 1130, 1221, 1388, 1482
TaqI TCGA 5 cut(s) 117, 224, 572, 710, 1554
TasI AATT 3 cut(s) 594, 669, 1546
TatI WGTACW 1 cut(s) 498
TauI GCSGC 4 cut(s) 486, 855, 1174, 1375
TfiI GAWTC 3 cut(s) 616, 1145, 1502
Tru1I TTAA 4 cut(s) 624, 1395, 1451, 1821
Tru9I TTAA 4 cut(s) 624, 1395, 1451, 1821
TscAI CASTG 6 cut(s) 964, 970, 1266, 1385, 1746, 1939
TseFI GTSAC 1 cut(s) 1159
TseI GCWGC 6 cut(s) 37, 133, 469, 1234, 1323, 1807
Tsp45I GTSAC 1 cut(s) 1159
TspDTI ATGAA 2 cut(s) 787, 1331
TspGWI ACGGA 1 cut(s) 1529
TspRI CASTG 6 cut(s) 964, 970, 1266, 1385, 1746, 1939
Tth111I GACNNNGTC 3 cut(s) 1697, 1759, 1775
VpaK11BI GGWCC 6 cut(s) 268, 524, 734, 950, 1255, 1732
XagI CCTNNNNNAGG 1 cut(s) 1700
XapI RAATTY 1 cut(s) 594
XcmI CCANNNNNNNNNTGG 1 cut(s) 48
XmiI GTMKAC 1 cut(s) 1302
XspI CTAG 4 cut(s) 1229, 1563, 1725, 1902
ZraI GACGTC 1 cut(s) 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.