pycom16g01050

Belongs to the group II decarboxylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006000
Physical Location & Seq
Forward (+)
641223 .. 642059
837 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 720 bp
ATGATAGCCAATCTTTTTAATGCACCACTCAAAGACGGCGAGGCTGCGACCGGAACGGGAACAGTAGGATCATCGGAGGCCATAATGCTGGCGGGCCTTGCATTCAAGAGGAAGTGGCAGAACAAGATGAAGGCTATCGGCAAACCCTATGACAAGCCCAACATAGTCACTGGTGCCAATGTTCAGGTTTGCTGGGAGAAATTTGCAAGGTACTTTGAAGTAGAACTGAAGGAAGTGAAAGTGAGGGAAGACTATTATGTAATGGACCCCGTCAAGGCTGTGGAGATGGTGGATGAAAACACCATCTGCGTTGCTGCCATCTTGGGATCCACTTACAATGGCGAATTCGAAGATGTCAAGCTCTTGAACGATCTTTTGATGGAAAAGAACAAGCAAACTGGATGGGACACTCCGATTCATGTTGATGCGGCGAGCGGTGGGTTCATCGCACCTTTCTTGTACCCTGATCTTGAATGGGACTTCCGTCTTCCACTGGTGAAGAGCATCAATGTGAGCGGTCATAAGTATGGCCTTGTTTACGCTGGAATTGGGTGGATTGTTTGGAGGAGCAAACAGGACTTGCCTGAAGACCTAATCTTCCACATCAATTACCTTGGAGCTGACCAGCCCACCTTCACCCTCAATTTCTCCAAAGGGTCTAGTCAGGTTCTTGCTCAATATTATCAGCTCATCCGACTTGGTTTTGAGGTGAGACTCTAA

Protein Analysis

240

Amino Acids

26.9

Weight (kDa)

5.49

Isoelectric Point (pI)

27.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 1 - 236 7.4e-72 Pyridoxal-dependent decarboxylase conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AccBSI CCGCTC 2 cut(s) 435, 516
AciI CCGC 4 cut(s) 92, 428, 435, 516
AclWI GGATC 3 cut(s) 76, 321, 334
AcsI RAATTY 2 cut(s) 200, 344
AcuI CTGAAG 2 cut(s) 248, 606
AfaI GTAC 2 cut(s) 212, 461
AfiI CCNNNNNNNGG 1 cut(s) 274
AgsI TTSAA 4 cut(s) 106, 218, 367, 473
AluBI AGCT 3 cut(s) 361, 620, 688
AluI AGCT 3 cut(s) 361, 620, 688
Alw26I GTCTC 1 cut(s) 706
AlwI GGATC 3 cut(s) 76, 321, 334
AoxI GGCC 3 cut(s) 78, 94, 529
ApeKI GCWGC 2 cut(s) 44, 314
ApoI RAATTY 2 cut(s) 200, 344
AspS9I GGNCC 2 cut(s) 94, 265
AsuHPI GGTGA 2 cut(s) 508, 628
AsuII TTCGAA 1 cut(s) 348
AvaII GGWCC 1 cut(s) 265
BamHI GGATCC 1 cut(s) 326
BanI GGYRCC 1 cut(s) 173
BbsI GAAGAC 3 cut(s) 255, 479, 594
BbvI GCAGC 2 cut(s) 31, 301
BccI CCATC 5 cut(s) 280, 311, 326, 373, 396
BceAI ACGGC 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 706
BfaI CTAG 1 cut(s) 660
BisI GCNGC 3 cut(s) 45, 315, 429
BlsI GCNGC 3 cut(s) 46, 316, 430
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 2 cut(s) 94, 265
BmiI GGNNCC 3 cut(s) 175, 267, 328
BmsI GCATC 2 cut(s) 415, 513
BoxI GACNNNNGTC 1 cut(s) 483
BpiI GAAGAC 3 cut(s) 255, 479, 594
Bpu14I TTCGAA 1 cut(s) 348
BsaJI CCNNGG 1 cut(s) 613
BsaWI WCCGGW 1 cut(s) 50
Bsc4I CCNNNNNNNGG 1 cut(s) 274
Bse1I ACTGG 3 cut(s) 175, 403, 498
BseDI CCNNGG 1 cut(s) 613
BseGI GGATG 3 cut(s) 298, 407, 690
BseLI CCNNNNNNNGG 1 cut(s) 274
BseNI ACTGG 3 cut(s) 175, 403, 498
BseRI GAGGAG 1 cut(s) 580
BseXI GCAGC 2 cut(s) 31, 301
BseYI CCCAGC 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 3 cut(s) 80, 96, 531
BshNI GGYRCC 1 cut(s) 173
BsiEI CGRYCG 1 cut(s) 51
BsiSI CCGG 1 cut(s) 51
BslFI GGGAC 2 cut(s) 419, 491
BslI CCNNNNNNNGG 1 cut(s) 274
BsmAI GTCTC 1 cut(s) 706
BsmFI GGGAC 2 cut(s) 419, 491
BsmI GAATGC 1 cut(s) 101
BsnI GGCC 3 cut(s) 80, 96, 531
Bsp119I TTCGAA 1 cut(s) 348
Bsp143I GATC 4 cut(s) 68, 326, 370, 466
BspACI CCGC 4 cut(s) 92, 428, 435, 516
BspANI GGCC 3 cut(s) 80, 96, 531
BspLI GGNNCC 3 cut(s) 175, 267, 328
BspPI GGATC 3 cut(s) 76, 321, 334
BspQI GCTCTTC 1 cut(s) 494
BspT104I TTCGAA 1 cut(s) 348
BspT107I GGYRCC 1 cut(s) 173
BsrBI CCGCTC 2 cut(s) 435, 516
BsrI ACTGG 3 cut(s) 175, 403, 498
BssECI CCNNGG 1 cut(s) 613
BssMI GATC 4 cut(s) 68, 326, 370, 466
BssT1I CCWWGG 1 cut(s) 613
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 1 cut(s) 494
BstBI TTCGAA 1 cut(s) 348
BstC8I GCNNGC 3 cut(s) 90, 94, 433
BstF5I GGATG 3 cut(s) 298, 407, 690
BstKTI GATC 4 cut(s) 71, 329, 373, 469
BstMAI GTCTC 1 cut(s) 706
BstMBI GATC 4 cut(s) 68, 326, 370, 466
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstPAI GACNNNNGTC 1 cut(s) 483
BstV1I GCAGC 2 cut(s) 31, 301
BstV2I GAAGAC 3 cut(s) 255, 479, 594
BstX2I RGATCY 1 cut(s) 326
BstXI CCANNNNNNTGG 1 cut(s) 88
BstYI RGATCY 1 cut(s) 326
BsuRI GGCC 3 cut(s) 80, 96, 531
BtgZI GCGATG 1 cut(s) 430
BtsCI GGATG 3 cut(s) 298, 407, 690
BtsIMutI CAGTG 2 cut(s) 168, 491
Cac8I GCNNGC 3 cut(s) 90, 94, 433
Cfr13I GGNCC 2 cut(s) 94, 265
Csp6I GTAC 2 cut(s) 211, 460
CviAII CATG 1 cut(s) 419
CviQI GTAC 2 cut(s) 211, 460
DpnI GATC 4 cut(s) 70, 328, 372, 468
DpnII GATC 4 cut(s) 68, 326, 370, 466
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
Eco130I CCWWGG 1 cut(s) 613
Eco47I GGWCC 1 cut(s) 265
Eco57I CTGAAG 2 cut(s) 248, 606
EcoRI GAATTC 1 cut(s) 344
EcoT14I CCWWGG 1 cut(s) 613
ErhI CCWWGG 1 cut(s) 613
FaeI CATG 1 cut(s) 422
FaiI YATR 7 cut(s) 83, 150, 164, 258, 420, 522, 528
FaqI GGGAC 2 cut(s) 419, 491
FatI CATG 1 cut(s) 418
FauI CCCGC 1 cut(s) 85
Fnu4HI GCNGC 3 cut(s) 45, 315, 429
FokI GGATG 3 cut(s) 305, 414, 677
Fsp4HI GCNGC 3 cut(s) 45, 315, 429
FspBI CTAG 1 cut(s) 660
GluI GCNGC 3 cut(s) 45, 315, 429
GsaI CCCAGC 1 cut(s) 196
HaeIII GGCC 3 cut(s) 80, 96, 531
HapII CCGG 1 cut(s) 51
Hin1II CATG 1 cut(s) 422
HinfI GANTC 2 cut(s) 415, 714
HpaII CCGG 1 cut(s) 51
HphI GGTGA 2 cut(s) 508, 628
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 3 cut(s) 76, 414, 695
Hpy188III TCNNGA 3 cut(s) 106, 364, 470
Hpy8I GTNNAC 1 cut(s) 538
HpyAV CCTTC 3 cut(s) 124, 223, 643
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4V TGCA 3 cut(s) 23, 101, 206
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
Hsp92II CATG 1 cut(s) 422
Kzo9I GATC 4 cut(s) 68, 326, 370, 466
LguI GCTCTTC 1 cut(s) 494
LmnI GCTCC 2 cut(s) 567, 617
Lsp1109I GCAGC 2 cut(s) 31, 301
LweI GCATC 2 cut(s) 415, 513
MaeI CTAG 1 cut(s) 660
MaeIII GTNAC 1 cut(s) 166
MalI GATC 4 cut(s) 70, 328, 372, 468
MbiI CCGCTC 2 cut(s) 435, 516
MboI GATC 4 cut(s) 68, 326, 370, 466
MboII GAAGA 6 cut(s) 260, 362, 479, 511, 589, 599
MflI RGATCY 1 cut(s) 326
MluCI AATT 5 cut(s) 200, 344, 546, 607, 643
MlyI GAGTC 1 cut(s) 708
MmeI TCCRAC 1 cut(s) 718
MnlI CCTC 7 cut(s) 34, 70, 102, 237, 558, 650, 700
MseI TTAA 1 cut(s) 18
MslI CAYNNNNRTG 3 cut(s) 423, 509, 525
MspI CCGG 1 cut(s) 51
Mva1269I GAATGC 1 cut(s) 101
MwoI GCNNNNNNNGC 1 cut(s) 98
NdeII GATC 4 cut(s) 68, 326, 370, 466
NlaIII CATG 1 cut(s) 422
NlaIV GGNNCC 3 cut(s) 175, 267, 328
NmuCI GTSAC 1 cut(s) 166
NspV TTCGAA 1 cut(s) 348
PciSI GCTCTTC 1 cut(s) 494
PctI GAATGC 1 cut(s) 101
PfeI GAWTC 1 cut(s) 415
PflFI GACNNNGTC 1 cut(s) 269
PkrI GCNGC 3 cut(s) 46, 316, 430
PleI GAGTC 1 cut(s) 708
PpsI GAGTC 1 cut(s) 708
PshAI GACNNNNGTC 1 cut(s) 483
PspFI CCCAGC 1 cut(s) 192
PspN4I GGNNCC 3 cut(s) 175, 267, 328
PspPI GGNCC 2 cut(s) 94, 265
PsuI RGATCY 1 cut(s) 326
PsyI GACNNNGTC 1 cut(s) 269
RsaI GTAC 2 cut(s) 212, 461
RsaNI GTAC 2 cut(s) 211, 460
RseI CAYNNNNRTG 3 cut(s) 423, 509, 525
SapI GCTCTTC 1 cut(s) 494
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 3 cut(s) 45, 315, 429
Sau3AI GATC 4 cut(s) 68, 326, 370, 466
Sau96I GGNCC 2 cut(s) 94, 265
SchI GAGTC 1 cut(s) 708
SfaNI GCATC 2 cut(s) 415, 513
SfuI TTCGAA 1 cut(s) 348
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 3 cut(s) 423, 509, 525
Sse9I AATT 5 cut(s) 200, 344, 546, 607, 643
SsiI CCGC 4 cut(s) 92, 428, 435, 516
SspI AATATT 1 cut(s) 680
SspMI CTAG 1 cut(s) 660
StyI CCWWGG 1 cut(s) 613
TaaI ACNGT 1 cut(s) 64
TaqI TCGA 1 cut(s) 348
TasI AATT 5 cut(s) 200, 344, 546, 607, 643
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 1 cut(s) 415
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 2 cut(s) 175, 498
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 2 cut(s) 44, 314
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 4 cut(s) 143, 309, 407, 433
TspGWI ACGGA 1 cut(s) 473
TspRI CASTG 2 cut(s) 175, 498
Tth111I GACNNNGTC 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 265
XapI RAATTY 2 cut(s) 200, 344
XspI CTAG 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.