RchiOBHm_Chr4g0406101

Belongs to the group II decarboxylase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0006000
Physical Location & Seq
Reverse (-)
27188205 .. 27189595
1391 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37753

Sequence Viewer

Length: 1140 bp
ATGATGGCAGATCTTTTCAATGCACCACATAAAGAGTCTGATGAGGCTGCCACCGGAACCGGAACAGTCGGGTCATCGGAGGCCATCCTGCTGGCAGGTCTCGCATTCAAGAGGAAGTGGCAGAATAAGAGGAAGGCTGAAGGAAAACCCTATGACAGGCCCAACATCGTTACTGGTGCCAATGTGCAGGTATGCTGGGAGAAATTTGCTAGGTATTTTGAAGTAGAACTGAAGGAAGTGAAGGTGACGGAAGACTATTATGTAATGGACCCTGTGAAAGCTGTGGAATTGGAAGATGAAAACACCATCTGCGTTGCTGCTATCTTGGGTTCAACTTACAATGGAGAGTTCGAAGATGTCAAGCTCTTGAATGATCTTTCGCTAGAGAAGAATAAGCAAACTGGATGGGATACCCCAATTCATGTCGATGCCGCTAGTGGTGGATTCATAGCGCCATTCTTGTATCCCGAGTTAGAATGGGATTTCCGTCTTCCACTGGTGAAGAGCATCAATGTGAGTGGTCATAAATATGGTCTTGTGTATGCTGGAGTTGGGTGGATCATTTGGAGGTCCAAAGAAGACTTGGCCGAAGACCTAATTTTTCACATGAATTATCTGGGAGCTGATCAACCCACCTTCACCCTCAATTTCTCTAAAGGGTCGAGTCAAATCATTGCTCAATACTATCAACTTATCCGACAGGGTTTTGAGGGATACCGCAACATCATGGAAAACTGTCACAACAATGCCATGGTGTTGAAGGCAGGGCTGGAAAAGACAGAGCGCTTCAAAATCTTGTCGAAGGACATCGGGGTTCCAGTGGTGGCCTTTTCTTTGAAAGACAGAAGCCACTACGATGAGTACATGGTGTCTGAGCATTTGCGTCGTTTTGGCTGGATTGTGCCGGCATATTCTATGCCGGAGGATGCTAAGCACGTTGCGTTGCTCCGTGTTGTGATAAGGGAAGACTTCTCTCGCACTCTAGCTGAGCGTCTTGTGCTCGACATCACCCATGTCTTGAAAGAGCTCGACAAACTGCCGCTGCCATCAGTCAGTCCAAATGGTGGTGATGACCAAGTGTGTATTAATGGCGGCATATGGAAACATGAACACTTTGGTGGCGAAAAAGAAATTTTCTGA

Protein Analysis

379

Amino Acids

42.96

Weight (kDa)

5.47

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 1 - 280 2.4e-81 Pyridoxal-dependent decarboxylase conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 86, 178
AccB1I GGYRCC 1 cut(s) 176
AccB7I CCANNNNNTGG 1 cut(s) 1064
AciI CCGC 4 cut(s) 432, 718, 1040, 1092
AclWI GGATC 1 cut(s) 566
AcoI YGGCCR 1 cut(s) 585
AcsI RAATTY 2 cut(s) 203, 1131
AcuI CTGAAG 2 cut(s) 159, 251
AfaI GTAC 1 cut(s) 863
AfeI AGCGCT 1 cut(s) 785
AfiI CCNNNNNNNGG 2 cut(s) 156, 1064
AgsI TTSAA 9 cut(s) 19, 109, 221, 333, 370, 760, 790, 838, 1021
AleI CACNNNNGTG 1 cut(s) 1116
AluBI AGCT 5 cut(s) 281, 364, 623, 986, 1027
AluI AGCT 5 cut(s) 281, 364, 623, 986, 1027
Alw21I GWGCWC 2 cut(s) 1002, 1029
Alw26I GTCTC 1 cut(s) 104
AlwI GGATC 1 cut(s) 566
Ama87I CYCGRG 1 cut(s) 467
Aor51HI AGCGCT 1 cut(s) 785
AoxI GGCC 4 cut(s) 81, 158, 585, 825
ApeKI GCWGC 3 cut(s) 47, 317, 1042
ApoI RAATTY 2 cut(s) 203, 1131
AseI ATTAAT 1 cut(s) 1086
AspLEI GCGC 2 cut(s) 454, 786
AspS9I GGNCC 3 cut(s) 159, 268, 570
AsuHPI GGTGA 5 cut(s) 256, 511, 631, 1000, 1079
AsuII TTCGAA 1 cut(s) 351
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 2 cut(s) 268, 570
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 1 cut(s) 1029
BbsI GAAGAC 5 cut(s) 258, 482, 585, 597, 972
Bbv12I GWGCWC 2 cut(s) 1002, 1029
BbvI GCAGC 3 cut(s) 34, 304, 1029
BccI CCATC 4 cut(s) 92, 314, 399, 1054
BcgI CGANNNNNNTGC 2 cut(s) 866, 900
BciVI GTATCC 3 cut(s) 403, 474, 707
BclI TGATCA 1 cut(s) 625
BcoDI GTCTC 1 cut(s) 104
BfaI CTAG 4 cut(s) 210, 383, 435, 983
BfoI RGCGCY 2 cut(s) 455, 787
BfuAI ACCTGC 2 cut(s) 86, 178
BfuI GTATCC 3 cut(s) 403, 474, 707
BglII AGATCT 1 cut(s) 10
BisI GCNGC 6 cut(s) 48, 318, 432, 1040, 1043, 1093
BlpI GCTNAGC 2 cut(s) 930, 987
BlsI GCNGC 6 cut(s) 49, 319, 433, 1041, 1044, 1094
Bme18I GGWCC 2 cut(s) 268, 570
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 3 cut(s) 159, 268, 570
BmiI GGNNCC 4 cut(s) 58, 178, 270, 816
BmsI GCATC 3 cut(s) 418, 516, 916
BpiI GAAGAC 5 cut(s) 258, 482, 585, 597, 972
BpmI CTGGAG 1 cut(s) 567
Bpu1102I GCTNAGC 2 cut(s) 930, 987
Bpu14I TTCGAA 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 750
BsaWI WCCGGW 2 cut(s) 53, 59
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 1064
Bse118I RCCGGY 1 cut(s) 904
Bse1I ACTGG 4 cut(s) 178, 406, 501, 818
Bse3DI GCAATG 1 cut(s) 672
BseDI CCNNGG 1 cut(s) 750
BseGI GGATG 3 cut(s) 84, 410, 931
BseLI CCNNNNNNNGG 2 cut(s) 156, 1064
BseMI GCAATG 1 cut(s) 672
BseMII CTCAG 2 cut(s) 864, 978
BseNI ACTGG 4 cut(s) 178, 406, 501, 818
BseXI GCAGC 3 cut(s) 34, 304, 1029
BseYI CCCAGC 1 cut(s) 195
BsgI GTGCAG 1 cut(s) 206
BshFI GGCC 4 cut(s) 83, 160, 587, 827
BshNI GGYRCC 1 cut(s) 176
BsiHKAI GWGCWC 2 cut(s) 1002, 1029
BsiHKCI CYCGRG 1 cut(s) 467
BsiSI CCGG 4 cut(s) 54, 60, 905, 920
BslI CCNNNNNNNGG 2 cut(s) 156, 1064
BsmAI GTCTC 1 cut(s) 104
BsmI GAATGC 1 cut(s) 104
BsnI GGCC 4 cut(s) 83, 160, 587, 827
Bso31I GGTCTC 1 cut(s) 104
BsoBI CYCGRG 1 cut(s) 467
Bsp119I TTCGAA 1 cut(s) 351
Bsp1286I GDGCHC 2 cut(s) 1002, 1029
Bsp143I GATC 4 cut(s) 10, 373, 558, 625
Bsp1720I GCTNAGC 2 cut(s) 930, 987
Bsp19I CCATGG 1 cut(s) 750
BspACI CCGC 4 cut(s) 432, 718, 1040, 1092
BspANI GGCC 4 cut(s) 83, 160, 587, 827
BspCNI CTCAG 2 cut(s) 865, 979
BspLI GGNNCC 4 cut(s) 58, 178, 270, 816
BspMI ACCTGC 2 cut(s) 86, 178
BspPI GGATC 1 cut(s) 566
BspQI GCTCTTC 1 cut(s) 497
BspT104I TTCGAA 1 cut(s) 351
BspT107I GGYRCC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 672
BsrFI RCCGGY 1 cut(s) 904
BsrI ACTGG 4 cut(s) 178, 406, 501, 818
BssAI RCCGGY 1 cut(s) 904
BssECI CCNNGG 1 cut(s) 750
BssMI GATC 4 cut(s) 10, 373, 558, 625
BssT1I CCWWGG 1 cut(s) 750
Bst4CI ACNGT 2 cut(s) 67, 737
Bst6I CTCTTC 1 cut(s) 497
BstBI TTCGAA 1 cut(s) 351
BstC8I GCNNGC 2 cut(s) 93, 906
BstDEI CTNAG 3 cut(s) 873, 930, 987
BstDSI CCRYGG 1 cut(s) 750
BstENI CCTNNNNNAGG 1 cut(s) 154
BstF5I GGATG 3 cut(s) 84, 410, 931
BstH2I RGCGCY 2 cut(s) 455, 787
BstHHI GCGC 2 cut(s) 454, 786
BstKTI GATC 4 cut(s) 13, 376, 561, 628
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 4 cut(s) 10, 373, 558, 625
BstMWI GCNNNNNNNGC 2 cut(s) 101, 997
BstV1I GCAGC 3 cut(s) 34, 304, 1029
BstV2I GAAGAC 5 cut(s) 258, 482, 585, 597, 972
BstX2I RGATCY 1 cut(s) 10
BstXI CCANNNNNNTGG 1 cut(s) 91
BstYI RGATCY 1 cut(s) 10
BsuI GTATCC 3 cut(s) 403, 474, 707
BsuRI GGCC 4 cut(s) 83, 160, 587, 827
BtgI CCRYGG 1 cut(s) 750
BtsCI GGATG 3 cut(s) 84, 410, 931
BtsIMutI CAGTG 2 cut(s) 494, 825
BveI ACCTGC 2 cut(s) 86, 178
Cac8I GCNNGC 2 cut(s) 93, 906
CfoI GCGC 2 cut(s) 454, 786
Cfr10I RCCGGY 1 cut(s) 904
Cfr13I GGNCC 3 cut(s) 159, 268, 570
CseI GACGC 2 cut(s) 872, 980
Csp6I GTAC 1 cut(s) 862
CspCI CAANNNNNGTGG 2 cut(s) 499, 534
CviAII CATG 7 cut(s) 422, 607, 727, 751, 865, 1013, 1106
CviQI GTAC 1 cut(s) 862
DdeI CTNAG 3 cut(s) 873, 930, 987
DpnI GATC 4 cut(s) 12, 375, 560, 627
DpnII GATC 4 cut(s) 10, 373, 558, 625
EaeI YGGCCR 1 cut(s) 585
Eam1104I CTCTTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 497
Ecl136II GAGCTC 1 cut(s) 1027
Eco130I CCWWGG 1 cut(s) 750
Eco24I GRGCYC 1 cut(s) 1029
Eco31I GGTCTC 1 cut(s) 104
Eco47I GGWCC 2 cut(s) 268, 570
Eco47III AGCGCT 1 cut(s) 785
Eco53kI GAGCTC 1 cut(s) 1027
Eco57I CTGAAG 2 cut(s) 159, 251
Eco88I CYCGRG 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 1027
EcoNI CCTNNNNNAGG 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 750
EcoT38I GRGCYC 1 cut(s) 1029
ErhI CCWWGG 1 cut(s) 750
FaeI CATG 7 cut(s) 425, 610, 730, 754, 868, 1016, 1109
FatI CATG 7 cut(s) 421, 606, 726, 750, 864, 1012, 1105
FauNDI CATATG 1 cut(s) 1097
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 6 cut(s) 48, 318, 432, 1040, 1043, 1093
FokI GGATG 3 cut(s) 71, 417, 938
FriOI GRGCYC 1 cut(s) 1029
Fsp4HI GCNGC 6 cut(s) 48, 318, 432, 1040, 1043, 1093
FspBI CTAG 4 cut(s) 210, 383, 435, 983
GlaI GCGC 2 cut(s) 453, 785
GluI GCNGC 6 cut(s) 48, 318, 432, 1040, 1043, 1093
GsaI CCCAGC 1 cut(s) 199
GsuI CTGGAG 1 cut(s) 567
HaeII RGCGCY 2 cut(s) 455, 787
HaeIII GGCC 4 cut(s) 83, 160, 587, 827
HapII CCGG 4 cut(s) 54, 60, 905, 920
HgaI GACGC 2 cut(s) 872, 980
HhaI GCGC 2 cut(s) 454, 786
Hin1II CATG 7 cut(s) 425, 610, 730, 754, 868, 1016, 1109
Hin6I GCGC 2 cut(s) 452, 784
HinP1I GCGC 2 cut(s) 452, 784
HinfI GANTC 3 cut(s) 35, 444, 664
HpaII CCGG 4 cut(s) 54, 60, 905, 920
HphI GGTGA 5 cut(s) 256, 511, 631, 1000, 1079
Hpy188I TCNGA 5 cut(s) 40, 79, 698, 874, 1139
Hpy188III TCNNGA 4 cut(s) 109, 367, 467, 1018
Hpy99I CGWCG 1 cut(s) 888
HpyAV CCTTC 7 cut(s) 127, 134, 226, 235, 646, 754, 796
HpyCH4III ACNGT 2 cut(s) 67, 737
HpyCH4IV ACGT 1 cut(s) 936
HpyCH4V TGCA 2 cut(s) 23, 187
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 997
HpyF3I CTNAG 3 cut(s) 873, 930, 987
HpySE526I ACGT 1 cut(s) 936
Hsp92II CATG 7 cut(s) 425, 610, 730, 754, 868, 1016, 1109
HspAI GCGC 2 cut(s) 452, 784
KroI GCCGGC 1 cut(s) 904
KroNI GCCGGC 1 cut(s) 906
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 4 cut(s) 10, 373, 558, 625
LguI GCTCTTC 1 cut(s) 497
LmnI GCTCC 2 cut(s) 620, 951
Lsp1109I GCAGC 3 cut(s) 34, 304, 1029
LweI GCATC 3 cut(s) 418, 516, 916
MaeI CTAG 4 cut(s) 210, 383, 435, 983
MaeII ACGT 1 cut(s) 936
MaeIII GTNAC 3 cut(s) 169, 244, 737
MalI GATC 4 cut(s) 12, 375, 560, 627
MboI GATC 4 cut(s) 10, 373, 558, 625
MboII GAAGA 9 cut(s) 263, 305, 365, 400, 482, 514, 590, 602, 977
MflI RGATCY 1 cut(s) 10
MhlI GDGCHC 2 cut(s) 1002, 1029
MluCI AATT 7 cut(s) 203, 287, 417, 597, 610, 646, 1131
MlyI GAGTC 2 cut(s) 44, 673
MmeI TCCRAC 1 cut(s) 721
MnlI CCTC 8 cut(s) 37, 73, 105, 123, 561, 653, 703, 916
MroNI GCCGGC 1 cut(s) 904
MseI TTAA 1 cut(s) 1086
MslI CAYNNNNRTG 6 cut(s) 426, 512, 528, 744, 855, 1116
MspA1I CMGCKG 1 cut(s) 1042
MspI CCGG 4 cut(s) 54, 60, 905, 920
Mva1269I GAATGC 1 cut(s) 104
MwoI GCNNNNNNNGC 2 cut(s) 101, 997
NaeI GCCGGC 1 cut(s) 906
NcoI CCATGG 1 cut(s) 750
NdeI CATATG 1 cut(s) 1097
NdeII GATC 4 cut(s) 10, 373, 558, 625
NgoMIV GCCGGC 1 cut(s) 904
NlaIII CATG 7 cut(s) 425, 610, 730, 754, 868, 1016, 1109
NlaIV GGNNCC 4 cut(s) 58, 178, 270, 816
NmuCI GTSAC 2 cut(s) 244, 737
NspV TTCGAA 1 cut(s) 351
OliI CACNNNNGTG 1 cut(s) 1116
PciSI GCTCTTC 1 cut(s) 497
PctI GAATGC 1 cut(s) 104
PdiI GCCGGC 1 cut(s) 906
PfeI GAWTC 1 cut(s) 444
PflMI CCANNNNNTGG 1 cut(s) 1064
PkrI GCNGC 6 cut(s) 49, 319, 433, 1041, 1044, 1094
PleI GAGTC 2 cut(s) 43, 672
PpsI GAGTC 2 cut(s) 43, 672
PshBI ATTAAT 1 cut(s) 1086
Psp124BI GAGCTC 1 cut(s) 1029
PspFI CCCAGC 1 cut(s) 195
PspN4I GGNNCC 4 cut(s) 58, 178, 270, 816
PspPI GGNCC 3 cut(s) 159, 268, 570
PsuI RGATCY 1 cut(s) 10
RsaI GTAC 1 cut(s) 863
RsaNI GTAC 1 cut(s) 862
RseI CAYNNNNRTG 6 cut(s) 426, 512, 528, 744, 855, 1116
SacI GAGCTC 1 cut(s) 1029
SapI GCTCTTC 1 cut(s) 497
SaqAI TTAA 1 cut(s) 1086
SatI GCNGC 6 cut(s) 48, 318, 432, 1040, 1043, 1093
Sau3AI GATC 4 cut(s) 10, 373, 558, 625
Sau96I GGNCC 3 cut(s) 159, 268, 570
SchI GAGTC 2 cut(s) 44, 673
SduI GDGCHC 2 cut(s) 1002, 1029
SfaNI GCATC 3 cut(s) 418, 516, 916
SfuI TTCGAA 1 cut(s) 351
SinI GGWCC 2 cut(s) 268, 570
SmiMI CAYNNNNRTG 6 cut(s) 426, 512, 528, 744, 855, 1116
Sse9I AATT 7 cut(s) 203, 287, 417, 597, 610, 646, 1131
SsiI CCGC 4 cut(s) 432, 718, 1040, 1092
SspMI CTAG 4 cut(s) 210, 383, 435, 983
SstI GAGCTC 1 cut(s) 1029
StyI CCWWGG 1 cut(s) 750
TaaI ACNGT 2 cut(s) 67, 737
TaiI ACGT 1 cut(s) 939
TaqI TCGA 6 cut(s) 351, 426, 662, 800, 1002, 1029
TasI AATT 7 cut(s) 203, 287, 417, 597, 610, 646, 1131
TatI WGTACW 1 cut(s) 861
TauI GCSGC 3 cut(s) 434, 1042, 1095
TfiI GAWTC 1 cut(s) 444
Tru1I TTAA 1 cut(s) 1086
Tru9I TTAA 1 cut(s) 1086
TscAI CASTG 2 cut(s) 501, 825
TseFI GTSAC 2 cut(s) 244, 737
TseI GCWGC 3 cut(s) 47, 317, 1042
Tsp45I GTSAC 2 cut(s) 244, 737
TspDTI ATGAA 5 cut(s) 312, 410, 436, 623, 1122
TspGWI ACGGA 3 cut(s) 263, 476, 938
TspRI CASTG 2 cut(s) 501, 825
Van91I CCANNNNNTGG 1 cut(s) 1064
VpaK11BI GGWCC 2 cut(s) 268, 570
VspI ATTAAT 1 cut(s) 1086
XagI CCTNNNNNAGG 1 cut(s) 154
XapI RAATTY 2 cut(s) 203, 1131
XcmI CCANNNNNNNNNTGG 1 cut(s) 580
XspI CTAG 4 cut(s) 210, 383, 435, 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.