pycom16g01390

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004318
Physical Location & Seq
Forward (+)
882412 .. 883062
651 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
ATGAAACCTTTGAGGGCTAGTATGGTGGCTGCGTGTCTTCAAGAGGTCCTGGGCATAGGAAAGAAGAGGCAACCAGGGAGAGTGGTGCCTAATGGATCGATCGTCCTCCAAAGTCTGCTCTGTGGGAAGAAAATTTTGGTGGTTGATGACAATTTGGTAAACAGGAGAGTTGCTGCAGGTGCACTAAAGAAGTTTGGTGCTTATGTAGAGTGCGTTGACAGTGGCAAAGCTGCATTGGCGTTGCTTCAATTACCGCACAATTTTGATGCTTGCTTCATGGATATTCAAATGCCTGAAATGGATGGGTTTGAGGCAACACGCAGAATCCGGCAGATGGAGAGCAAGGCAAATGTTGAGATGAATGGTGGATTTGAAGCACTCGCAAGAAAGGGTGACTGGCATTTGCCTGTGTTAGCTATGACGGCGGATGTTATTCATGCCACCTATGATGAGTGCCTGAAGTGTGGGATGGATGGATACGTCTCAAAGCCCTTCGAGGAAGAGAATCTCTATCAAGCCGTTGCCAAGTTCTTCAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004721 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006464 GO:0006468 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0007275 GO:0008150 GO:0008152 GO:0008272 GO:0009267 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009743 GO:0009744 GO:0009755 GO:0009784 GO:0009790 GO:0009884 GO:0009885 GO:0009987 GO:0009991 GO:0010015 GO:0010029 GO:0010033 GO:0010035 GO:0010086 GO:0012505 GO:0015698 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016787 GO:0016788 GO:0016791 GO:0018106 GO:0018193 GO:0018202 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022622 GO:0023052 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032870 GO:0033500 GO:0033554 GO:0034285 GO:0035556 GO:0036211 GO:0038023 GO:0042221 GO:0042578 GO:0042592 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048509 GO:0048580 GO:0048598 GO:0048731 GO:0048831 GO:0048856 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071470 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0072348 GO:0098542 GO:0099402 GO:0104004 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

181

Amino Acids

19.84

Weight (kDa)

8.19

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 45 - 118 1.3e-15 Response regulator receiver domain
Response_reg PF00072 130 - 174 1.5e-06 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 167
Acc36I ACCTGC 1 cut(s) 167
AccB1I GGYRCC 1 cut(s) 85
AciI CCGC 2 cut(s) 254, 425
AclWI GGATC 1 cut(s) 103
AcsI RAATTY 1 cut(s) 132
AcuI CTGAAG 1 cut(s) 479
AfiI CCNNNNNNNGG 1 cut(s) 334
AgsI TTSAA 5 cut(s) 41, 248, 287, 374, 535
AjnI CCWGG 2 cut(s) 48, 73
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 2 cut(s) 230, 416
AluI AGCT 2 cut(s) 230, 416
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 1 cut(s) 487
Alw44I GTGCAC 1 cut(s) 180
AlwI GGATC 1 cut(s) 103
ApaLI GTGCAC 1 cut(s) 180
ApeKI GCWGC 3 cut(s) 29, 173, 230
ApoI RAATTY 1 cut(s) 132
AspS9I GGNCC 1 cut(s) 46
AsuHPI GGTGA 1 cut(s) 404
AvaII GGWCC 1 cut(s) 46
BaeGI GKGCMC 1 cut(s) 184
BanI GGYRCC 1 cut(s) 85
BbsI GAAGAC 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 3 cut(s) 16, 160, 217
BccI CCATC 4 cut(s) 296, 328, 463, 467
BceAI ACGGC 2 cut(s) 438, 503
BciT130I CCWGG 2 cut(s) 50, 75
BciVI GTATCC 1 cut(s) 470
BcoDI GTCTC 1 cut(s) 487
BfaI CTAG 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 174
BfuAI ACCTGC 1 cut(s) 167
BfuI GTATCC 1 cut(s) 470
BisI GCNGC 3 cut(s) 30, 174, 231
BlsI GCNGC 3 cut(s) 31, 175, 232
Bme1390I CCNGG 2 cut(s) 50, 75
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 2 cut(s) 50, 75
BmsI GCATC 1 cut(s) 256
BpiI GAAGAC 1 cut(s) 29
Bsa29I ATCGAT 1 cut(s) 98
BsaJI CCNNGG 2 cut(s) 49, 74
Bsc4I CCNNNNNNNGG 1 cut(s) 334
Bse1I ACTGG 1 cut(s) 401
BseBI CCWGG 2 cut(s) 50, 75
BseCI ATCGAT 1 cut(s) 98
BseDI CCNNGG 2 cut(s) 49, 74
BseGI GGATG 4 cut(s) 307, 433, 474, 478
BseLI CCNNNNNNNGG 1 cut(s) 334
BseNI ACTGG 1 cut(s) 401
BseSI GKGCMC 1 cut(s) 184
BseXI GCAGC 3 cut(s) 16, 160, 217
Bsh1285I CGRYCG 1 cut(s) 102
BshNI GGYRCC 1 cut(s) 85
BshVI ATCGAT 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 184
BsiSI CCGG 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 334
BsmAI GTCTC 1 cut(s) 487
BsmBI CGTCTC 1 cut(s) 487
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 2 cut(s) 95, 99
BspACI CCGC 2 cut(s) 254, 425
BspDI ATCGAT 1 cut(s) 98
BspLI GGNNCC 1 cut(s) 87
BspMAI CTGCAG 1 cut(s) 178
BspMI ACCTGC 1 cut(s) 167
BspPI GGATC 1 cut(s) 103
BspT107I GGYRCC 1 cut(s) 85
BsrI ACTGG 1 cut(s) 401
BssECI CCNNGG 2 cut(s) 49, 74
BssMI GATC 2 cut(s) 95, 99
Bst2UI CCWGG 2 cut(s) 50, 75
Bst4CI ACNGT 1 cut(s) 221
Bst6I CTCTTC 2 cut(s) 59, 495
BstC8I GCNNGC 1 cut(s) 271
BstF5I GGATG 4 cut(s) 307, 433, 474, 478
BstKTI GATC 2 cut(s) 98, 102
BstMAI GTCTC 1 cut(s) 487
BstMBI GATC 2 cut(s) 95, 99
BstMCI CGRYCG 1 cut(s) 102
BstMWI GCNNNNNNNGC 3 cut(s) 179, 236, 422
BstNI CCWGG 2 cut(s) 50, 75
BstSCI CCNGG 2 cut(s) 48, 73
BstSFI CTRYAG 1 cut(s) 174
BstSLI GKGCMC 1 cut(s) 184
BstV1I GCAGC 3 cut(s) 16, 160, 217
BstV2I GAAGAC 1 cut(s) 29
Bsu15I ATCGAT 1 cut(s) 98
BsuI GTATCC 1 cut(s) 470
BsuTUI ATCGAT 1 cut(s) 98
BtsCI GGATG 4 cut(s) 307, 433, 474, 478
BtsIMutI CAGTG 1 cut(s) 226
BveI ACCTGC 1 cut(s) 167
Cac8I GCNNGC 1 cut(s) 271
Cfr13I GGNCC 1 cut(s) 46
ClaI ATCGAT 1 cut(s) 98
CviAII CATG 2 cut(s) 277, 437
CviJI RGCY 6 cut(s) 17, 29, 230, 416, 490, 518
CviKI_1 RGCY 6 cut(s) 17, 29, 230, 416, 490, 518
DpnI GATC 2 cut(s) 97, 101
DpnII GATC 2 cut(s) 95, 99
Eam1104I CTCTTC 2 cut(s) 59, 495
EarI CTCTTC 2 cut(s) 59, 495
EciI GGCGGA 1 cut(s) 440
Eco47I GGWCC 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 479
EcoO109I RGGNCCY 1 cut(s) 46
EcoRII CCWGG 2 cut(s) 48, 73
Esp3I CGTCTC 1 cut(s) 487
FaeI CATG 2 cut(s) 280, 440
FaiI YATR 7 cut(s) 23, 56, 204, 278, 419, 438, 447
FatI CATG 2 cut(s) 276, 436
Fnu4HI GCNGC 3 cut(s) 30, 174, 231
FokI GGATG 4 cut(s) 314, 440, 481, 485
Fsp4HI GCNGC 3 cut(s) 30, 174, 231
FspBI CTAG 1 cut(s) 18
GluI GCNGC 3 cut(s) 30, 174, 231
HapII CCGG 1 cut(s) 328
Hin1II CATG 2 cut(s) 280, 440
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 2 cut(s) 324, 505
HpaII CCGG 1 cut(s) 328
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 3 cut(s) 160, 182, 217
Hpy188III TCNNGA 1 cut(s) 41
Hpy8I GTNNAC 3 cut(s) 160, 182, 217
HpyAV CCTTC 1 cut(s) 502
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 3 cut(s) 176, 182, 233
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 236, 422
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 2 cut(s) 280, 440
Kzo9I GATC 2 cut(s) 95, 99
Lsp1109I GCAGC 3 cut(s) 16, 160, 217
LweI GCATC 1 cut(s) 256
MaeI CTAG 1 cut(s) 18
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 1 cut(s) 392
MalI GATC 2 cut(s) 97, 101
MboI GATC 2 cut(s) 95, 99
MboII GAAGA 5 cut(s) 29, 76, 139, 512, 523
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 4 cut(s) 132, 151, 248, 259
MnlI CCTC 6 cut(s) 6, 37, 60, 116, 304, 490
MseI TTAA 1 cut(s) 541
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 2 cut(s) 50, 75
MvaI CCWGG 2 cut(s) 50, 75
MwoI GCNNNNNNNGC 3 cut(s) 179, 236, 422
NdeII GATC 2 cut(s) 95, 99
NlaIII CATG 2 cut(s) 280, 440
NlaIV GGNNCC 1 cut(s) 87
NmuCI GTSAC 1 cut(s) 392
PaqCI CACCTGC 1 cut(s) 167
PfeI GAWTC 2 cut(s) 324, 505
PkrI GCNGC 3 cut(s) 31, 175, 232
Ple19I CGATCG 1 cut(s) 102
PpuMI RGGWCCY 1 cut(s) 46
Psp5II RGGWCCY 1 cut(s) 46
Psp6I CCWGG 2 cut(s) 48, 73
PspGI CCWGG 2 cut(s) 48, 73
PspN4I GGNNCC 1 cut(s) 87
PspPI GGNCC 1 cut(s) 46
PspPPI RGGWCCY 1 cut(s) 46
PstI CTGCAG 1 cut(s) 178
PvuI CGATCG 1 cut(s) 102
SaqAI TTAA 1 cut(s) 541
SatI GCNGC 3 cut(s) 30, 174, 231
Sau3AI GATC 2 cut(s) 95, 99
Sau96I GGNCC 1 cut(s) 46
ScrFI CCNGG 2 cut(s) 50, 75
SduI GDGCHC 1 cut(s) 184
SetI ASST 7 cut(s) 10, 48, 181, 232, 418, 446, 483
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 1 cut(s) 174
SinI GGWCC 1 cut(s) 46
Sse9I AATT 4 cut(s) 132, 151, 248, 259
SsiI CCGC 2 cut(s) 254, 425
SspMI CTAG 1 cut(s) 18
StyD4I CCNGG 2 cut(s) 48, 73
TaaI ACNGT 1 cut(s) 221
TaiI ACGT 1 cut(s) 483
TaqI TCGA 2 cut(s) 98, 495
TasI AATT 4 cut(s) 132, 151, 248, 259
TfiI GAWTC 2 cut(s) 324, 505
Tru1I TTAA 1 cut(s) 541
Tru9I TTAA 1 cut(s) 541
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 3 cut(s) 29, 173, 230
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 4 cut(s) 17, 265, 374, 425
TspRI CASTG 1 cut(s) 226
VneI GTGCAC 1 cut(s) 180
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 1 cut(s) 132
XspI CTAG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.