pycom16g05480

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011931
Physical Location & Seq
Reverse (-)
3532572 .. 3535201
2630 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1116 bp
ATGGAAGACGATGAAGAAGCTCCACCTCTCGCCGTTGAAATCGACCAACCGACGGAACCCTACTCTCGGCAGCAGCCCAATTCATCTATTCGCATAGACGACGACGTTTCGGTCGGTGTCACCGTCATCACCGGCTATCTTGGCGCCGGAAAATCCACTCTGGTTAATCATATATTGAATTCCCAACACGGGAAGAGAATTGCTGTGATATTAAACGAGTTTGGTGAGGAAATTGGCGTGGAGAGAGCGATGATTAACGAAGGAGATGGCGGTGCGCTTGTCGAAGAGTGGGTGGAGTTAGCAAATGGGTGTATTTGTTGCACTGTTAAGCACAGTTTGGTGCAGGCATTGGAGCAACTTGTTCAGAGAAAAGAAAGACTTGATCATATATTGCTGGAGACTACTGGGTTAGCAAACCCAGCTCCGCTTGCTTCGGTTCTGTGGTTGGATGATAATCTAGAATCGTCTGTCAAACTTGATTCAATTATCACTGTTGTAGATGCCAAGAACCTCCGTTTTCAGCTCAGTGAAAATCGAAGTTCATCTTCATTTCCTGAAGCATATCTTCAGATTGCGTTTGCGGATGTTATAATTCTTAACAAGGTTGATTTGGTTTCTCCAGAGGATTCTGAGGAACTGGAGAAGGAAATACGTAGTATTAACTCACTTGCTAGTATCATTCGTTCTGTTCGTGGTCAAGTTGACTTGTCCAATATATTGGACTGTCGGGCCTATGATGCCACGCATGCTACCCATTTAGAATCATTATTGGAAGAAAGTCGTACTCTGTCTACAAGCGATCTTCATGATATTGGTGTGCGAACTTTATGCATTTCCGAGCTACGGCAGGTTGATCTTGAAAAGGTTCGTTTGTGGATTGAGGAGATTCTTTGGGAGAAGAAAGATGGCATGGATGTATACCGCTGCAAAGGGGTTTTGAGCATTCATAATTCAAATCAATTACATACTTTGCAGGGAGTGAAGGAGATATATGAGATTGTGCCAACTCGTGATTGGAAAACGGAAGAAACTCAGACGAACAAGATAGTGTTCATAGGTCATAATCTAAATGAGAATGTTCTCGCCGAGTCTTTTAGGTCGTGCGTAGTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C KTI12 cobW

Protein Analysis

372

Amino Acids

41.48

Weight (kDa)

4.69

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 40 - 227 2.2e-58 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 274 - 369 1e-17 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011931)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26520
fragaria_vesca FvH4_4g30300
malus_domestica MD13G1064000.v1.1 MD16G1062700.v1.1
prunus_persica Prupe.1G284700_v2.0.a1
pyrus_communis pycom16g05480
rosa_chinensis RchiOBHm_Chr4g0438581
rosa_laevigata RLG00000006349
rosa_multiflora Rmu_co8071018.1_g000001 Rmu_sc0002327.1_g000002 Rmu_sc0020430.1_g000001
rosa_roxburghii Rroxscaffold_5G00379590
rosa_rugosa Rorug04G0311700
rosa_samantha Rh4AG362100 Rh4BG373500 Rh4CG387400 Rh4DG368200
rosa_wichuraiana Rw0G006680 Rw0G016630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 590
AasI GACNNNNNNGTC 1 cut(s) 110
Acc36I ACCTGC 1 cut(s) 838
AccB1I GGYRCC 1 cut(s) 143
AccI GTMKAC 2 cut(s) 791, 918
AciI CCGC 4 cut(s) 270, 425, 581, 922
AcsI RAATTY 1 cut(s) 178
AcuI CTGAAG 2 cut(s) 551, 576
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 1 cut(s) 784
AfiI CCNNNNNNNGG 4 cut(s) 52, 66, 189, 843
AgsI TTSAA 5 cut(s) 38, 178, 483, 860, 954
AluBI AGCT 4 cut(s) 20, 422, 523, 841
AluI AGCT 4 cut(s) 20, 422, 523, 841
Alw26I GTCTC 1 cut(s) 392
AoxI GGCC 1 cut(s) 729
ApeKI GCWGC 3 cut(s) 70, 73, 924
ApoI RAATTY 1 cut(s) 178
Asp700I GAANNNNTTC 1 cut(s) 864
AspLEI GCGC 2 cut(s) 146, 277
AspS9I GGNCC 1 cut(s) 729
AsuHPI GGTGA 3 cut(s) 112, 121, 236
BanI GGYRCC 1 cut(s) 143
BauI CACGAG 1 cut(s) 1008
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 3 cut(s) 82, 85, 911
BccI CCATC 2 cut(s) 260, 899
BceAI ACGGC 2 cut(s) 17, 860
BcgI CGANNNNNNTGC 2 cut(s) 810, 844
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 392
BfaI CTAG 2 cut(s) 458, 672
BfoI RGCGCY 1 cut(s) 147
BfuAI ACCTGC 1 cut(s) 838
BisI GCNGC 3 cut(s) 71, 74, 925
BlsI GCNGC 3 cut(s) 72, 75, 926
BmgT120I GGNCC 1 cut(s) 729
BmiI GGNNCC 2 cut(s) 57, 145
BmrI ACTGGG 1 cut(s) 414
BmsI GCATC 2 cut(s) 490, 727
BmuI ACTGGG 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 3 cut(s) 416, 603, 659
BsaAI YACGTR 1 cut(s) 653
BsaBI GATNNNNATC 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 144
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 66, 189, 843
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 2 cut(s) 409, 642
Bse8I GATNNNNATC 1 cut(s) 453
BseGI GGATG 3 cut(s) 454, 589, 919
BseJI GATNNNNATC 1 cut(s) 453
BseLI CCNNNNNNNGG 4 cut(s) 52, 66, 189, 843
BseMII CTCAG 3 cut(s) 538, 621, 1046
BseNI ACTGG 2 cut(s) 409, 642
BseRI GAGGAG 1 cut(s) 896
BseXI GCAGC 3 cut(s) 82, 85, 911
BseYI CCCAGC 1 cut(s) 418
BsgI GTGCAG 1 cut(s) 362
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 731
BshNI GGYRCC 1 cut(s) 143
BsiEI CGRYCG 1 cut(s) 114
BsiSI CCGG 2 cut(s) 132, 147
BslI CCNNNNNNNGG 4 cut(s) 52, 66, 189, 843
BsmAI GTCTC 1 cut(s) 392
BsmI GAATGC 1 cut(s) 942
BsnI GGCC 1 cut(s) 731
Bsp143I GATC 3 cut(s) 382, 799, 853
BspACI CCGC 4 cut(s) 270, 425, 581, 922
BspANI GGCC 1 cut(s) 731
BspCNI CTCAG 3 cut(s) 537, 622, 1045
BspHI TCATGA 1 cut(s) 805
BspLI GGNNCC 2 cut(s) 57, 145
BspMI ACCTGC 1 cut(s) 838
BspT107I GGYRCC 1 cut(s) 143
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 2 cut(s) 409, 642
BssAI RCCGGY 1 cut(s) 131
BssMI GATC 3 cut(s) 382, 799, 853
BssNAI GTATAC 1 cut(s) 919
BssNI GRCGYC 1 cut(s) 144
BssSI CACGAG 1 cut(s) 1008
Bst1107I GTATAC 1 cut(s) 919
Bst2BI CACGAG 1 cut(s) 1008
Bst4CI ACNGT 5 cut(s) 124, 325, 335, 493, 725
Bst6I CTCTTC 2 cut(s) 188, 279
BstACI GRCGYC 1 cut(s) 144
BstBAI YACGTR 1 cut(s) 653
BstC8I GCNNGC 3 cut(s) 345, 429, 747
BstDEI CTNAG 3 cut(s) 524, 630, 1032
BstF5I GGATG 3 cut(s) 454, 589, 919
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 2 cut(s) 146, 277
BstKTI GATC 3 cut(s) 385, 802, 856
BstMAI GTCTC 1 cut(s) 392
BstMBI GATC 3 cut(s) 382, 799, 853
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 5 cut(s) 141, 419, 428, 737, 746
BstNSI RCATGY 1 cut(s) 749
BstSNI TACGTA 1 cut(s) 653
BstV1I GCAGC 3 cut(s) 82, 85, 911
BstV2I GAAGAC 1 cut(s) 12
BstXI CCANNNNNNTGG 1 cut(s) 718
BstZ17I GTATAC 1 cut(s) 919
BsuRI GGCC 1 cut(s) 731
BtgZI GCGATG 1 cut(s) 263
BtsCI GGATG 3 cut(s) 454, 589, 919
BtsIMutI CAGTG 3 cut(s) 321, 489, 532
BveI ACCTGC 1 cut(s) 838
Cac8I GCNNGC 3 cut(s) 345, 429, 747
CciI TCATGA 1 cut(s) 805
CfoI GCGC 2 cut(s) 146, 277
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 729
Csp6I GTAC 1 cut(s) 783
CviAII CATG 3 cut(s) 746, 806, 910
CviJI RGCY 7 cut(s) 20, 76, 135, 422, 523, 731, 841
CviKI_1 RGCY 7 cut(s) 20, 76, 135, 422, 523, 731, 841
CviQI GTAC 1 cut(s) 783
DdeI CTNAG 3 cut(s) 524, 630, 1032
DinI GGCGCC 1 cut(s) 145
DpnI GATC 3 cut(s) 384, 801, 855
DpnII GATC 3 cut(s) 382, 799, 853
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
Eam1104I CTCTTC 2 cut(s) 188, 279
EarI CTCTTC 2 cut(s) 188, 279
Eco105I TACGTA 1 cut(s) 653
Eco57I CTGAAG 2 cut(s) 551, 576
EcoRI GAATTC 1 cut(s) 178
EcoT22I ATGCAT 1 cut(s) 833
EgeI GGCGCC 1 cut(s) 145
EheI GGCGCC 1 cut(s) 145
FaeI CATG 3 cut(s) 749, 809, 913
FalI AAGNNNNNCTT 6 cut(s) 363, 395, 529, 561, 549, 581
FatI CATG 3 cut(s) 745, 805, 909
FbaI TGATCA 1 cut(s) 382
FblI GTMKAC 2 cut(s) 791, 918
Fnu4HI GCNGC 3 cut(s) 71, 74, 925
FokI GGATG 3 cut(s) 461, 596, 926
Fsp4HI GCNGC 3 cut(s) 71, 74, 925
FspBI CTAG 2 cut(s) 458, 672
GlaI GCGC 2 cut(s) 145, 276
GluI GCNGC 3 cut(s) 71, 74, 925
GsaI CCCAGC 1 cut(s) 422
GsuI CTGGAG 3 cut(s) 416, 603, 659
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 1 cut(s) 731
HapII CCGG 2 cut(s) 132, 147
HhaI GCGC 2 cut(s) 146, 277
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 3 cut(s) 749, 809, 913
Hin6I GCGC 2 cut(s) 144, 275
HinP1I GCGC 2 cut(s) 144, 275
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HinfI GANTC 6 cut(s) 461, 479, 626, 761, 886, 1088
HpaII CCGG 2 cut(s) 132, 147
HphI GGTGA 3 cut(s) 112, 121, 236
Hpy166II GTNNAC 3 cut(s) 703, 792, 919
Hpy188I TCNGA 5 cut(s) 366, 570, 631, 838, 1035
Hpy188III TCNNGA 6 cut(s) 458, 554, 620, 806, 857, 1010
Hpy8I GTNNAC 3 cut(s) 703, 792, 919
Hpy99I CGWCG 3 cut(s) 55, 104, 107
HpyAV CCTTC 3 cut(s) 254, 637, 976
HpyCH4III ACNGT 5 cut(s) 124, 325, 335, 493, 725
HpyCH4IV ACGT 2 cut(s) 105, 652
HpyCH4V TGCA 5 cut(s) 321, 343, 831, 927, 973
HpyF10VI GCNNNNNNNGC 5 cut(s) 141, 419, 428, 737, 746
HpyF3I CTNAG 3 cut(s) 524, 630, 1032
HpySE526I ACGT 2 cut(s) 105, 652
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 3 cut(s) 749, 809, 913
HspAI GCGC 2 cut(s) 144, 275
KasI GGCGCC 1 cut(s) 143
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 3 cut(s) 382, 799, 853
LmnI GCTCC 3 cut(s) 25, 352, 427
Lsp1109I GCAGC 3 cut(s) 82, 85, 911
LweI GCATC 2 cut(s) 490, 727
MaeI CTAG 2 cut(s) 458, 672
MaeII ACGT 2 cut(s) 105, 652
MaeIII GTNAC 1 cut(s) 118
MalI GATC 3 cut(s) 384, 801, 855
MboI GATC 3 cut(s) 382, 799, 853
MluCI AATT 8 cut(s) 79, 178, 198, 231, 483, 591, 949, 959
Mly113I GGCGCC 1 cut(s) 144
MlyI GAGTC 1 cut(s) 1097
MmeI TCCRAC 1 cut(s) 426
MnlI CCTC 6 cut(s) 36, 220, 521, 616, 625, 874
Mph1103I ATGCAT 1 cut(s) 833
MroXI GAANNNNTTC 1 cut(s) 864
MseI TTAA 6 cut(s) 165, 212, 255, 327, 597, 660
MspA1I CMGCKG 1 cut(s) 924
MspI CCGG 2 cut(s) 132, 147
Mva1269I GAATGC 1 cut(s) 942
MwoI GCNNNNNNNGC 5 cut(s) 141, 419, 428, 737, 746
NarI GGCGCC 1 cut(s) 144
NdeII GATC 3 cut(s) 382, 799, 853
NlaIII CATG 3 cut(s) 749, 809, 913
NlaIV GGNNCC 2 cut(s) 57, 145
NmeAIII GCCGAG 2 cut(s) 46, 1111
NmuCI GTSAC 1 cut(s) 118
NsiI ATGCAT 1 cut(s) 833
NspI RCATGY 1 cut(s) 749
PaeI GCATGC 1 cut(s) 749
PagI TCATGA 1 cut(s) 805
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 688
PctI GAATGC 1 cut(s) 942
PdmI GAANNNNTTC 1 cut(s) 864
PfeI GAWTC 5 cut(s) 461, 479, 626, 761, 886
PkrI GCNGC 3 cut(s) 72, 75, 926
PleI GAGTC 1 cut(s) 1096
PluTI GGCGCC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 1096
Ppu21I YACGTR 1 cut(s) 653
PsiI TTATAA 1 cut(s) 590
PspFI CCCAGC 1 cut(s) 418
PspN4I GGNNCC 2 cut(s) 57, 145
PspPI GGNCC 1 cut(s) 729
PsrI GAACNNNNNNTAC 2 cut(s) 667, 699
RsaI GTAC 1 cut(s) 784
RsaNI GTAC 1 cut(s) 783
SaqAI TTAA 6 cut(s) 165, 212, 255, 327, 597, 660
SatI GCNGC 3 cut(s) 71, 74, 925
Sau3AI GATC 3 cut(s) 382, 799, 853
Sau96I GGNCC 1 cut(s) 729
SchI GAGTC 1 cut(s) 1097
SfaNI GCATC 2 cut(s) 490, 727
SfoI GGCGCC 1 cut(s) 145
SnaBI TACGTA 1 cut(s) 653
SphI GCATGC 1 cut(s) 749
Sse9I AATT 8 cut(s) 79, 178, 198, 231, 483, 591, 949, 959
SsiI CCGC 4 cut(s) 270, 425, 581, 922
SspDI GGCGCC 1 cut(s) 143
SspMI CTAG 2 cut(s) 458, 672
TaaI ACNGT 5 cut(s) 124, 325, 335, 493, 725
TaiI ACGT 2 cut(s) 108, 655
TaqI TCGA 3 cut(s) 42, 282, 535
TaqII GACCGA 1 cut(s) 100
TasI AATT 8 cut(s) 79, 178, 198, 231, 483, 591, 949, 959
TfiI GAWTC 5 cut(s) 461, 479, 626, 761, 886
Tru1I TTAA 6 cut(s) 165, 212, 255, 327, 597, 660
Tru9I TTAA 6 cut(s) 165, 212, 255, 327, 597, 660
TscAI CASTG 3 cut(s) 328, 496, 532
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 3 cut(s) 70, 73, 924
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 7 cut(s) 27, 72, 531, 537, 794, 935, 1042
TspGWI ACGGA 3 cut(s) 68, 503, 1037
TspRI CASTG 3 cut(s) 328, 496, 532
XapI RAATTY 1 cut(s) 178
XbaI TCTAGA 1 cut(s) 457
XceI RCATGY 1 cut(s) 749
XcmI CCANNNNNNNNNTGG 1 cut(s) 1011
XmiI GTMKAC 2 cut(s) 791, 918
XmnI GAANNNNTTC 1 cut(s) 864
XspI CTAG 2 cut(s) 458, 672
Zsp2I ATGCAT 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.