pycom16g07660

AP-1 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004423
Physical Location & Seq
Reverse (-)
5028491 .. 5032042
3552 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1173 bp
ATGAATGACATACTTGCCCAGGTGGCGACAAAAACCGAGTCAAACAAAAATGCAGGAAATGCAATTCTATATGAATGTGTTGAAACTATCATGAGCATTGAAGATAGTGGTGGCTTACGTGTGCTTGCCATCAATATCTTGGGAAGATTCTTATCAAATCGTGACAACAATATCAGATACGTTGCATTAAATATGCTGATGAAGGCTATTACAGTTGATGCTCAAGCTGTGCAGAGGCATCGGGCAACTATCTTGGAATGTGTAAAGGATTCAGATGCCTCAATTCGGAAAAGGGCCCTTGAGCTTGTTTATGTGCTTGTGAATGAGAGCAATGTGAAGCCTCTAACAAAAGAGCTAATTGACTATTTGGAAATAAGTGATCAAGAATTCAAGGGAGATCTTACTGCAAAAATTTGCTCCATTGTTACAAAGTTTTCCCCCGAGAAGATTTGGTACATTGACCAGATGCTAAAGGTTCTCTCTGAGGCTGGAAATTTTGTAAAAGATGAAGTATGGCACGCACTTATTGTTGTGATTAGCAATGCTTCTGATCTCCATGGATATACGGTTAGGGCGTTATACCGAGCATTCCAATTATCAGCTGAACAGGAAAGTCTTGTTCGAGTCGCAATTTGGTGCATTGGAGAATATGGTGACATGTTGGTTAACAATGTTGGCATGCTCGATATAGAGGACCCAGTGACAGTAACAGAGTCTGACGCTGTGGATGTTATAGAGGTTGCAATTAAGCACCATACCTCGGATCTGACTACCAAAGCAATGGGTTTGATTGCTCTGTTGAAGCTATCTTCACGTTTCCCATCTTGTTCAGAGAGGATCGAGGATATAGTTGTTCAATACAAAGGAAGCCTTGTCCTCGAATTGCAGCAAAGATCCCTTGAATTGAACTCTATTATTGCGAAGCATCAGAACATTAGATCTACACTGGTTGAACGGATGCCAGTTTTGGATGAGGCAACTTTCATTGGAAAGAAGGCTGGTTCAATGCAATCTACAATTTCTTCTCCTTCTGGTGCTTCAATCAATCTACCAAATGGAGTTGCCAAACCTTCTGCAGCTCCTCTTGTGGATCTACTTGATCTAGGTTCAGATGATGTGCCAGCACCTAGCTCCTCTGGTGGTGATTTGCTTCATCGATCTTCTTGGTGTTGA

Protein Analysis

391

Amino Acids

42.83

Weight (kDa)

5.16

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 1 - 309 4.2e-76 Adaptin N terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 771, 845, 888, 1098
AcsI RAATTY 3 cut(s) 386, 411, 493
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 2 cut(s) 285, 760
AflIII ACRYGT 2 cut(s) 118, 657
AjnI CCWGG 1 cut(s) 18
AluBI AGCT 7 cut(s) 227, 304, 355, 602, 805, 1079, 1131
AluI AGCT 7 cut(s) 227, 304, 355, 602, 805, 1079, 1131
AlwI GGATC 4 cut(s) 771, 845, 888, 1098
AlwNI CAGNNNCTG 1 cut(s) 716
Ama87I CYCGRG 1 cut(s) 440
AoxI GGCC 1 cut(s) 294
ApaI GGGCCC 1 cut(s) 298
ApeKI GCWGC 2 cut(s) 886, 1076
ApoI RAATTY 3 cut(s) 386, 411, 493
ArsI GACNNNNNNTTYG 2 cut(s) 23, 55
AspS9I GGNCC 3 cut(s) 294, 295, 694
AsuHPI GGTGA 2 cut(s) 665, 1154
AvaI CYCGRG 1 cut(s) 440
AvaII GGWCC 1 cut(s) 694
BaeGI GKGCMC 1 cut(s) 298
BanII GRGCYC 1 cut(s) 298
BarI GAAGNNNNNNTAC 2 cut(s) 437, 469
BbvI GCAGC 2 cut(s) 898, 1088
BccI CCATC 2 cut(s) 137, 829
BcgI CGANNNNNNTGC 2 cut(s) 221, 255
BciT130I CCWGG 1 cut(s) 20
BclI TGATCA 1 cut(s) 379
BfaI CTAG 2 cut(s) 1103, 1128
BfmI CTRYAG 1 cut(s) 1074
BglI GCCNNNNNGGC 1 cut(s) 23
BglII AGATCT 2 cut(s) 397, 938
BisI GCNGC 2 cut(s) 887, 1077
BlsI GCNGC 2 cut(s) 888, 1078
Bme1390I CCNGG 1 cut(s) 20
Bme18I GGWCC 1 cut(s) 694
BmeT110I CYCGRG 1 cut(s) 440
BmgT120I GGNCC 3 cut(s) 294, 295, 694
BmiI GGNNCC 2 cut(s) 296, 696
BmrFI CCNGG 1 cut(s) 20
BmrI ACTGGG 1 cut(s) 692
BmsI GCATC 6 cut(s) 208, 247, 265, 456, 934, 948
BmuI ACTGGG 1 cut(s) 692
BpuEI CTTGAG 2 cut(s) 207, 320
Bsa29I ATCGAT 1 cut(s) 1156
BsaAI YACGTR 1 cut(s) 119
BsaBI GATNNNNATC 1 cut(s) 151
BsaJI CCNNGG 3 cut(s) 18, 556, 759
Bsc4I CCNNNNNNNGG 2 cut(s) 285, 760
Bse1I ACTGG 3 cut(s) 698, 951, 962
Bse3DI GCAATG 3 cut(s) 337, 547, 786
Bse8I GATNNNNATC 1 cut(s) 151
BseBI CCWGG 1 cut(s) 20
BseCI ATCGAT 1 cut(s) 1156
BseDI CCNNGG 3 cut(s) 18, 556, 759
BseGI GGATG 3 cut(s) 733, 963, 976
BseJI GATNNNNATC 1 cut(s) 151
BseLI CCNNNNNNNGG 2 cut(s) 285, 760
BseMI GCAATG 3 cut(s) 337, 547, 786
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 3 cut(s) 698, 951, 962
BseRI GAGGAG 2 cut(s) 1071, 1123
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 2 cut(s) 898, 1088
BsgI GTGCAG 1 cut(s) 251
BshFI GGCC 1 cut(s) 296
BshVI ATCGAT 1 cut(s) 1156
BsiHKCI CYCGRG 1 cut(s) 440
BslI CCNNNNNNNGG 2 cut(s) 285, 760
BsmI GAATGC 1 cut(s) 587
BsnI GGCC 1 cut(s) 296
BsoBI CYCGRG 1 cut(s) 440
Bsp120I GGGCCC 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 298
Bsp19I CCATGG 1 cut(s) 556
BspANI GGCC 1 cut(s) 296
BspCNI CTCAG 1 cut(s) 475
BspDI ATCGAT 1 cut(s) 1156
BspHI TCATGA 1 cut(s) 90
BspLI GGNNCC 2 cut(s) 296, 696
BspMAI CTGCAG 1 cut(s) 1078
BspPI GGATC 4 cut(s) 771, 845, 888, 1098
BsrDI GCAATG 3 cut(s) 337, 547, 786
BsrI ACTGG 3 cut(s) 698, 951, 962
BssECI CCNNGG 3 cut(s) 18, 556, 759
BssT1I CCWWGG 1 cut(s) 556
Bst2UI CCWGG 1 cut(s) 20
Bst4CI ACNGT 3 cut(s) 214, 568, 706
BstAPI GCANNNNNTGC 1 cut(s) 59
BstBAI YACGTR 1 cut(s) 119
BstC8I GCNNGC 4 cut(s) 126, 519, 680, 1122
BstDEI CTNAG 1 cut(s) 483
BstDSI CCRYGG 1 cut(s) 556
BstF5I GGATG 3 cut(s) 733, 963, 976
BstMWI GCNNNNNNNGC 2 cut(s) 23, 59
BstNI CCWGG 1 cut(s) 20
BstNSI RCATGY 2 cut(s) 661, 682
BstSCI CCNGG 1 cut(s) 18
BstSFI CTRYAG 1 cut(s) 1074
BstSLI GKGCMC 1 cut(s) 298
BstV1I GCAGC 2 cut(s) 898, 1088
BstX2I RGATCY 5 cut(s) 397, 763, 893, 938, 1090
BstXI CCANNNNNNTGG 1 cut(s) 781
BstYI RGATCY 5 cut(s) 397, 763, 893, 938, 1090
Bsu15I ATCGAT 1 cut(s) 1156
BsuRI GGCC 1 cut(s) 296
BsuTUI ATCGAT 1 cut(s) 1156
BtgI CCRYGG 1 cut(s) 556
BtsCI GGATG 3 cut(s) 733, 963, 976
BtsIMutI CAGTG 2 cut(s) 705, 944
Cac8I GCNNGC 4 cut(s) 126, 519, 680, 1122
CaiI CAGNNNCTG 1 cut(s) 716
CciI TCATGA 1 cut(s) 90
Cfr13I GGNCC 3 cut(s) 294, 295, 694
ClaI ATCGAT 1 cut(s) 1156
CseI GACGC 1 cut(s) 728
Csp6I GTAC 1 cut(s) 454
CviAII CATG 4 cut(s) 91, 557, 658, 679
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 1 cut(s) 483
Eco130I CCWWGG 1 cut(s) 556
Eco24I GRGCYC 1 cut(s) 298
Eco47I GGWCC 1 cut(s) 694
Eco88I CYCGRG 1 cut(s) 440
EcoO109I RGGNCCY 3 cut(s) 294, 295, 694
EcoRI GAATTC 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 18
EcoT14I CCWWGG 1 cut(s) 556
EcoT38I GRGCYC 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 556
FaeI CATG 4 cut(s) 94, 560, 661, 682
FalI AAGNNNNNCTT 2 cut(s) 855, 887
FatI CATG 4 cut(s) 90, 556, 657, 678
FbaI TGATCA 1 cut(s) 379
Fnu4HI GCNGC 2 cut(s) 887, 1077
FokI GGATG 3 cut(s) 740, 970, 983
FriOI GRGCYC 1 cut(s) 298
Fsp4HI GCNGC 2 cut(s) 887, 1077
FspBI CTAG 2 cut(s) 1103, 1128
GluI GCNGC 2 cut(s) 887, 1077
HaeIII GGCC 1 cut(s) 296
HgaI GACGC 1 cut(s) 728
Hin1II CATG 4 cut(s) 94, 560, 661, 682
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HinfI GANTC 5 cut(s) 38, 147, 269, 624, 713
HpaI GTTAAC 1 cut(s) 667
HphI GGTGA 2 cut(s) 665, 1154
Hpy166II GTNNAC 1 cut(s) 667
Hpy188III TCNNGA 3 cut(s) 91, 161, 383
Hpy8I GTNNAC 1 cut(s) 667
HpyAV CCTTC 4 cut(s) 196, 988, 1038, 1080
HpyCH4III ACNGT 3 cut(s) 214, 568, 706
HpyCH4IV ACGT 3 cut(s) 118, 180, 814
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 59
HpyF3I CTNAG 1 cut(s) 483
HpySE526I ACGT 3 cut(s) 118, 180, 814
Hsp92II CATG 4 cut(s) 94, 560, 661, 682
Ksp22I TGATCA 1 cut(s) 379
KspAI GTTAAC 1 cut(s) 667
LmnI GCTCC 3 cut(s) 422, 1084, 1136
Lsp1109I GCAGC 2 cut(s) 898, 1088
LweI GCATC 6 cut(s) 208, 247, 265, 456, 934, 948
MaeI CTAG 2 cut(s) 1103, 1128
MaeII ACGT 3 cut(s) 118, 180, 814
MaeIII GTNAC 5 cut(s) 161, 424, 653, 700, 706
MboII GAAGA 6 cut(s) 113, 156, 457, 801, 1014, 1152
MflI RGATCY 5 cut(s) 397, 763, 893, 938, 1090
MhlI GDGCHC 1 cut(s) 298
MlyI GAGTC 3 cut(s) 47, 633, 722
MseI TTAA 3 cut(s) 188, 666, 747
MspA1I CMGCKG 1 cut(s) 602
MspR9I CCNGG 1 cut(s) 20
Mva1269I GAATGC 1 cut(s) 587
MvaI CCWGG 1 cut(s) 20
MwoI GCNNNNNNNGC 2 cut(s) 23, 59
NcoI CCATGG 1 cut(s) 556
NlaIII CATG 4 cut(s) 94, 560, 661, 682
NlaIV GGNNCC 2 cut(s) 296, 696
NmuCI GTSAC 3 cut(s) 161, 653, 700
NspI RCATGY 2 cut(s) 661, 682
PaeI GCATGC 1 cut(s) 682
PagI TCATGA 1 cut(s) 90
PciI ACATGT 1 cut(s) 657
PcsI WCGNNNNNNNCGW 1 cut(s) 572
PctI GAATGC 1 cut(s) 587
PfeI GAWTC 2 cut(s) 147, 269
PkrI GCNGC 2 cut(s) 888, 1078
PleI GAGTC 3 cut(s) 46, 632, 721
PpsI GAGTC 3 cut(s) 46, 632, 721
Ppu21I YACGTR 1 cut(s) 119
PpuMI RGGWCCY 1 cut(s) 694
PscI ACATGT 1 cut(s) 657
Psp5II RGGWCCY 1 cut(s) 694
Psp6I CCWGG 1 cut(s) 18
PspGI CCWGG 1 cut(s) 18
PspN4I GGNNCC 2 cut(s) 296, 696
PspOMI GGGCCC 1 cut(s) 294
PspPI GGNCC 3 cut(s) 294, 295, 694
PspPPI RGGWCCY 1 cut(s) 694
PstI CTGCAG 1 cut(s) 1078
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 5 cut(s) 397, 763, 893, 938, 1090
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
SaqAI TTAA 3 cut(s) 188, 666, 747
SatI GCNGC 2 cut(s) 887, 1077
Sau96I GGNCC 3 cut(s) 294, 295, 694
SchI GAGTC 3 cut(s) 47, 633, 722
ScrFI CCNGG 1 cut(s) 20
SduI GDGCHC 1 cut(s) 298
SfaNI GCATC 6 cut(s) 208, 247, 265, 456, 934, 948
SfcI CTRYAG 1 cut(s) 1074
SinI GGWCC 1 cut(s) 694
SmlI CTYRAG 2 cut(s) 222, 299
SmoI CTYRAG 2 cut(s) 222, 299
SphI GCATGC 1 cut(s) 682
SspMI CTAG 2 cut(s) 1103, 1128
StyD4I CCNGG 1 cut(s) 18
StyI CCWWGG 1 cut(s) 556
TaaI ACNGT 3 cut(s) 214, 568, 706
TaiI ACGT 3 cut(s) 121, 183, 817
TaqI TCGA 5 cut(s) 622, 684, 840, 879, 1156
TfiI GAWTC 2 cut(s) 147, 269
Tru1I TTAA 3 cut(s) 188, 666, 747
Tru9I TTAA 3 cut(s) 188, 666, 747
TscAI CASTG 2 cut(s) 705, 951
TseFI GTSAC 3 cut(s) 161, 653, 700
TseI GCWGC 2 cut(s) 886, 1076
Tsp45I GTSAC 3 cut(s) 161, 653, 700
TspDTI ATGAA 6 cut(s) 17, 87, 215, 522, 973, 1142
TspGWI ACGGA 1 cut(s) 970
TspRI CASTG 2 cut(s) 705, 951
VpaK11BI GGWCC 1 cut(s) 694
XapI RAATTY 3 cut(s) 386, 411, 493
XceI RCATGY 2 cut(s) 661, 682
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XspI CTAG 2 cut(s) 1103, 1128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.