Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0004423

Physical Location & Seq

Genomic Coordinates Forward (+)

67478466 .. 67489638

Gene Structure 11173 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG437100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2712 bp

ATGAATCCCTTCTCTTCCGGAACGCGCCTGAGGGACATGATTCGGGCTATACGTGCTTGCAAAACTGCAGCAGAGGAACGTGGTGTTGTAAGAAAAGAGTGTGCTGCTATTCGTGCTGCCATAAATGAAAATGATAATGACTACAGGCACCGTAACCTGGCTAAGCTCATGTTCATCCACATGCTGGGATACCCTACACATTTTGGTCAAATGGAGTGCCTGAAGTTGATCGCGTCTGCTGGTTTTCCTGAGAAGAGAATAGGGTATCTTGGCCTCATGCTGCTTCTCGATGAAAGACAAGAAGTTCTGATGTTGGTCACAAACTCGTTGAAACAAGATCTGAATCACTCAAACCAGTATATTGTCGGACTTGCTCTTTGTGCTATGGGAAATATTTGTTCAGCAGAGATGGCTCGTGATCTTGCACCAGAAGTAGAGAGATTGCTTCAATTTCGAGATCCCAATATCCGGAAAAAGGCAGCATTGTGCTCCATAAGGATTATAAAGAAAGTCCCTGAACTGGCGGAAAATTTTATTAATCCTGCTGCTGCCTTACTTAGAGAAAAGCATCATGGCGTTTTAATCACAGGAGTTCAACTTTGTACAGAGCTTTGTAAAGCCAGTGAAGAAGCGCTCGAATACTTTAGAATGAAGTGCACAGAGGGTTTGGTCAAGACATTAAAGGATCTAGTGAACAGCCCATATGCTCCTGAGTACGACATTGCTGGGATCACGGATCCTTTCCTCCATATTAGATTGCTAAAGCTTTTGCGTGTGTTGGGTCAAGGAGATGAGGATGCTAGTGAATGTATGAATGACATACTTGCCCAGGTGGCTACCAAAACTGAGTCAAACAAAAATGCAGGGAATGCAATTCTATACGAATGTGTAGCAACTATAATGAGCATCGAAGACAATGGTGGCTTACGTGTGCTTGCCATCAATATTTTGGGAAGATTTTTATCGAATCGTGACAATAATATCAGATATGTTGCGTTAAACATGCTGATGAGAGCCATTACAGTTGATGCTCAAGCTGTACAGAGGCATCGGGCAACTATCTTGGAATGTGTAAAGGATTTAGATGCTTCAATTCGTAAAAGGGCCCTTGAACTTGTTTATGTTCTTGTGAATGAAAGTAACGTGAAACCTCTAACAAAAGAGCTCATTGACTATTTGGAAGTAAGTGATCAAGATTTCAAGGGAGATCTTACAGCAAAAATTTGCTCCCTTGTCAAGAAGTTTTCTCCTGAGAAGATTTGGTACATTGATCAGATGCTGAAGGTTCTCTCTGAGGCTGGAAATTTTGTACAAGATGAAGTATGGCACGCAGTTATTGTTGTGATAACCAATTCACCTGATCTTCATGGGTATGCGGTTAGGGCTTTATACAGAGCACTCCAAACATCAGCTGATCAGGAAACTTTGGTTCGAGTAGCAGTTTGGTGCATCGGAGAATATGGTGACATGTTGGTAAATAATATTGGCATGCTTGATGTAGAGGATCCCATAACGGTAACAGAGAGTGATGCTGTGGATATCATAGAAATTGCCCTTAAGCACCATACCTCGGATCTTACTACCAAAGCTATGGCTTTGATTGCTCTCTTAAAGCTATCTTCACGTTTCCCATCTTGTTCAGAGTATGTTTCTATGTTAATTTGCTTGTTTTATTATTATCTGATTTTAATTTACACGAAGCCTGATTGTTCATTGAATGGCCTATGTAGGAGGATCAAAGAAATCATAGTTCAGTACAAAGGAAGCCTTGTGCTTGAATTGCAGCAAAGATCCATTGAATTGAATTCTATTATTGCGAAGCATCAGAACATTAGACCTACACTGGTTGAGAGGATGCCAACTTTGGATTTGTTAGAGTGGGAGGAAAAGCAGAGTGGTTCTATACCAAATACTACTGGTTCAACTCCTGCTCATGCTTCAATCAACATACCAAATGGAGTTGTCAAACCTTCTTCAGCTCCTCTTGTGGATTTACTTGATCTAAATTCAGATGATGTGCCAGCACCTAGCTCCTCTGGTGGTGATTCTTTTCATGGTCTTCTCGATATTGATTTGTCAGGAGCTCCTAAACAATCGGGTGTAAATCATTCTCCAAACAATGGCACTGATGCTCTAATGGATCTCTTGTCTACTGCTACACCTACACAAAGCAGCTCAGCTATCTCTGATCTGTTAAACTCTGGTCAAGATAACAAAACACCTGTTTCACCATTAGAGGTACTATCATCTCCATCTTCAAACTCCATACAACCCACTTCTTCTGCTGGAGCTATAGATTTGCTGGACAGCTTTGCCACCAACTCACCAATACAAGAAAACAATGGCCCTGCGTATCCATCTGTAGTGGCATTTGAGAGCAGCAATTTAAGGATAGGGTTTAACTTCTCGAAGCTGCCTGGAAATCCACAAACAACAATAATCAAGGCTACATTCACTAATTTGTCACCTAGCGTCTATACGGATTTTATTTTTCAGGCAGCTGTCCCAAAGTTCCTGCAACTGCACTTAGAGCCAGCTAGCGGCAATACTCTTCCGGCAAGTGGTAATGAGTCCATCACACAGACTTTGAGAGTTACCAATAGCCAACATGGAAAGAAAAGTCTTGTCATGCGCATAAGGATAGCTTACAAGATGAACAACAAAGATGTCTTGGAGGAAGGACAAATCAACAATTTTCCCCAGGGTTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0006810 GO:0008150 GO:0012505 GO:0012506 GO:0012510 GO:0016020 GO:0016192 GO:0030117 GO:0030118 GO:0030119 GO:0030120 GO:0030121 GO:0030125 GO:0030130 GO:0030131 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0048475 GO:0051179 GO:0051234 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04142 map04142

Pfam Domains

Protein Families

Adaptin_N Alpha_adaptinC2

Protein Analysis

903

Amino Acids

99.56

Weight (kDa)

5.79

Isoelectric Point (pI)

44.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Adaptin_N	PF01602	27 - 608	2e-150	Adaptin N terminal region
Alpha_adaptinC2	PF02883	787 - 900	2.3e-33	Adaptin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23900 AT1G23900 AT1G23900 AT1G60070 AT1G60070
fragaria_vesca	FvH4_4g34830 FvH4_4g34830 FvH4_4g34830 FvH4_4g34830 FvH4_4g34830 FvH4_4g34830 FvH4_4g34830 FvH4_4g34830 FvH4_4g34830
malus_domestica	MD13G1089300.v1.1 MD16G1089500.v1.1
prunus_persica	Prupe.1G256500_v2.0.a1
pyrus_communis	pycom13g07770 pycom16g07660
rosa_chinensis	RchiOBHm_Chr4g0444171
rosa_laevigata	RLG00000005850
rosa_multiflora	Rmu_sc0034408.1_g000001 Rmu_ssc0000462.1_g000001
rosa_roxburghii	Rroxscaffold_5G00384760
rosa_rugosa	Rorug04G0350700 Rorug04G0350700
rosa_samantha	Rh4AG411400 Rh4BG422800 Rh4CG154500 Rh4CG437100 Rh4DG418100
rosa_wichuraiana	Rw4G035340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	503
Acc16I	TGCGCA	1 cut(s)	2633
AccB1I	GGYRCC	1 cut(s)	147
AccB7I	CCANNNNNTGG	2 cut(s)	184, 2120
AccI	GTMKAC	1 cut(s)	2150
AccII	CGCG	2 cut(s)	25, 233
AccIII	TCCGGA	2 cut(s)	17, 468
AciI	CCGC	3 cut(s)	524, 1376, 2541
AcsI	RAATTY	5 cut(s)	529, 1221, 1303, 1804, 2005
AcuI	CTGAAG	3 cut(s)	242, 1301, 1959
AfaI	GTAC	7 cut(s)	604, 716, 1041, 1265, 1311, 1757, 2241
AfeI	AGCGCT	1 cut(s)	633
AfiI	CCNNNNNNNGG	9 cut(s)	157, 184, 468, 475, 520, 1570, 2120, 2540, 2561
AflII	CTTAAG	1 cut(s)	1556
AflIII	ACRYGT	2 cut(s)	928, 1467
AjnI	CCWGG	4 cut(s)	156, 828, 2416, 2700
Alw21I	GWGCWC	5 cut(s)	491, 659, 1167, 1399, 2086
Alw44I	GTGCAC	1 cut(s)	655
AlwNI	CAGNNNCTG	1 cut(s)	1279
Aor13HI	TCCGGA	2 cut(s)	17, 468
Aor51HI	AGCGCT	1 cut(s)	633
AoxI	GGCC	4 cut(s)	271, 1104, 1720, 2344
ApaI	GGGCCC	1 cut(s)	1108
ApaLI	GTGCAC	1 cut(s)	655
ApoI	RAATTY	5 cut(s)	529, 1221, 1303, 1804, 2005
ArsI	GACNNNNNNTTYG	2 cut(s)	360, 392
AseI	ATTAAT	1 cut(s)	537
Asp700I	GAANNNNTTC	1 cut(s)	8
AspLEI	GCGC	3 cut(s)	27, 634, 2634
AspS9I	GGNCC	3 cut(s)	1104, 1105, 2345
AsuHPI	GGTGA	6 cut(s)	1347, 1475, 2054, 2220, 2316, 2457
AsuNHI	GCTAGC	1 cut(s)	2537
AxyI	CCTNAGG	1 cut(s)	29
BaeGI	GKGCMC	2 cut(s)	659, 1108
BamHI	GGATCC	2 cut(s)	736, 1504
BanI	GGYRCC	1 cut(s)	147
BanII	GRGCYC	3 cut(s)	1108, 1167, 2086
BarI	GAAGNNNNNNTAC	2 cut(s)	1247, 1279
BauI	CACGAG	1 cut(s)	414
BbsI	GAAGAC	2 cut(s)	918, 2051
Bbv12I	GWGCWC	5 cut(s)	491, 659, 1167, 1399, 2086
BccI	CCATC	6 cut(s)	403, 947, 1639, 2260, 2365, 2582
BciT130I	CCWGG	4 cut(s)	158, 830, 2418, 2702
BciVI	GTATCC	2 cut(s)	182, 2364
BclI	TGATCA	3 cut(s)	1189, 1270, 1414
BfaI	CTAG	5 cut(s)	689, 801, 2028, 2469, 2538
BfmI	CTRYAG	4 cut(s)	66, 142, 2292, 2361
BfoI	RGCGCY	1 cut(s)	635
BfrI	CTTAAG	1 cut(s)	1556
BfuI	GTATCC	2 cut(s)	182, 2364
BglI	GCCNNNNNGGC	1 cut(s)	833
BglII	AGATCT	2 cut(s)	337, 1207
BlpI	GCTNAGC	2 cut(s)	162, 2176
Bme1390I	CCNGG	4 cut(s)	158, 830, 2418, 2702
BmgT120I	GGNCC	3 cut(s)	1104, 1105, 2345
BmiI	GGNNCC	4 cut(s)	149, 738, 1106, 1506
BmrFI	CCNGG	4 cut(s)	158, 830, 2418, 2702
BmtI	GCTAGC	1 cut(s)	2541
BpiI	GAAGAC	2 cut(s)	918, 2051
BpmI	CTGGAG	1 cut(s)	2307
Bpu1102I	GCTNAGC	2 cut(s)	162, 2176
BpuEI	CTTGAG	1 cut(s)	1017
BsaAI	YACGTR	2 cut(s)	53, 929
BsaBI	GATNNNNATC	2 cut(s)	342, 961
BsaJI	CCNNGG	4 cut(s)	828, 1569, 2700, 2701
BsaWI	WCCGGW	2 cut(s)	17, 468
Bsc4I	CCNNNNNNNGG	9 cut(s)	157, 184, 468, 475, 520, 1570, 2120, 2540, 2561
Bse1I	ACTGG	5 cut(s)	355, 525, 621, 1848, 1921
Bse21I	CCTNAGG	1 cut(s)	29
Bse3DI	GCAATG	1 cut(s)	720
Bse8I	GATNNNNATC	2 cut(s)	342, 961
BseAI	TCCGGA	2 cut(s)	17, 468
BseBI	CCWGG	4 cut(s)	158, 830, 2418, 2702
BseDI	CCNNGG	4 cut(s)	828, 1569, 2700, 2701
BseGI	GGATG	3 cut(s)	174, 802, 1860
BseJI	GATNNNNATC	2 cut(s)	342, 961
BseLI	CCNNNNNNNGG	9 cut(s)	157, 184, 468, 475, 520, 1570, 2120, 2540, 2561
BseMI	GCAATG	1 cut(s)	720
BseMII	CTCAG	7 cut(s)	20, 240, 702, 837, 1242, 1284, 2190
BseNI	ACTGG	5 cut(s)	355, 525, 621, 1848, 1921
BseRI	GAGGAG	2 cut(s)	1971, 2023
BseSI	GKGCMC	2 cut(s)	659, 1108
BseYI	CCCAGC	2 cut(s)	184, 725
BsgI	GTGCAG	1 cut(s)	2507
Bsh1236I	CGCG	2 cut(s)	25, 233
BshFI	GGCC	4 cut(s)	273, 1106, 1722, 2346
BshNI	GGYRCC	1 cut(s)	147
BsiHKAI	GWGCWC	5 cut(s)	491, 659, 1167, 1399, 2086
BsiSI	CCGG	3 cut(s)	18, 469, 2555
BslFI	GGGAC	3 cut(s)	47, 497, 2489
BslI	CCNNNNNNNGG	9 cut(s)	157, 184, 468, 475, 520, 1570, 2120, 2540, 2561
BsmFI	GGGAC	3 cut(s)	47, 497, 2489
BsmI	GAATGC	1 cut(s)	874
BsnI	GGCC	4 cut(s)	273, 1106, 1722, 2346
Bsp120I	GGGCCC	1 cut(s)	1104
Bsp1286I	GDGCHC	6 cut(s)	491, 659, 1108, 1167, 1399, 2086
Bsp13I	TCCGGA	2 cut(s)	17, 468
Bsp1407I	TGTACA	3 cut(s)	602, 1039, 1309
Bsp1720I	GCTNAGC	2 cut(s)	162, 2176
BspACI	CCGC	3 cut(s)	524, 1376, 2541
BspANI	GGCC	4 cut(s)	273, 1106, 1722, 2346
BspCNI	CTCAG	7 cut(s)	21, 241, 703, 838, 1243, 1285, 2189
BspEI	TCCGGA	2 cut(s)	17, 468
BspFNI	CGCG	2 cut(s)	25, 233
BspLI	GGNNCC	4 cut(s)	149, 738, 1106, 1506
BspMAI	CTGCAG	1 cut(s)	70
BspOI	GCTAGC	1 cut(s)	2541
BspT107I	GGYRCC	1 cut(s)	147
BspTI	CTTAAG	1 cut(s)	1556
BsrDI	GCAATG	1 cut(s)	720
BsrGI	TGTACA	3 cut(s)	602, 1039, 1309
BsrI	ACTGG	5 cut(s)	355, 525, 621, 1848, 1921
BssECI	CCNNGG	4 cut(s)	828, 1569, 2700, 2701
BssSI	CACGAG	1 cut(s)	414
Bst2BI	CACGAG	1 cut(s)	414
Bst2UI	CCWGG	4 cut(s)	158, 830, 2418, 2702
Bst4CI	ACNGT	3 cut(s)	152, 1024, 1516
Bst6I	CTCTTC	3 cut(s)	19, 248, 2556
BstAFI	CTTAAG	1 cut(s)	1556
BstAPI	GCANNNNNTGC	1 cut(s)	869
BstAUI	TGTACA	3 cut(s)	602, 1039, 1309
BstBAI	YACGTR	2 cut(s)	53, 929
BstC8I	GCNNGC	7 cut(s)	58, 936, 1329, 1490, 2022, 2535, 2539
BstF5I	GGATG	3 cut(s)	174, 802, 1860
BstFNI	CGCG	2 cut(s)	25, 233
BstH2I	RGCGCY	1 cut(s)	635
BstHHI	GCGC	3 cut(s)	27, 634, 2634
BstNI	CCWGG	4 cut(s)	158, 830, 2418, 2702
BstNSI	RCATGY	4 cut(s)	184, 1006, 1471, 1492
BstSCI	CCNGG	4 cut(s)	156, 828, 2416, 2700
BstSFI	CTRYAG	4 cut(s)	66, 142, 2292, 2361
BstSLI	GKGCMC	2 cut(s)	659, 1108
BstUI	CGCG	2 cut(s)	25, 233
BstV2I	GAAGAC	2 cut(s)	918, 2051
BstX2I	RGATCY	9 cut(s)	337, 457, 685, 736, 1207, 1504, 1573, 1790, 2140
BstXI	CCANNNNNNTGG	1 cut(s)	1591
BstYI	RGATCY	9 cut(s)	337, 457, 685, 736, 1207, 1504, 1573, 1790, 2140
Bsu36I	CCTNAGG	1 cut(s)	29
BsuI	GTATCC	2 cut(s)	182, 2364
BsuRI	GGCC	4 cut(s)	273, 1106, 1722, 2346
BtsCI	GGATG	3 cut(s)	174, 802, 1860
BtsIMutI	CAGTG	3 cut(s)	628, 1841, 2124
Cac8I	GCNNGC	7 cut(s)	58, 936, 1329, 1490, 2022, 2535, 2539
CaiI	CAGNNNCTG	1 cut(s)	1279
CfoI	GCGC	3 cut(s)	27, 634, 2634
Cfr13I	GGNCC	3 cut(s)	1104, 1105, 2345
CseI	GACGC	2 cut(s)	222, 2461
Csp6I	GTAC	7 cut(s)	603, 715, 1040, 1264, 1310, 1756, 2240
CviQI	GTAC	7 cut(s)	603, 715, 1040, 1264, 1310, 1756, 2240
Eam1104I	CTCTTC	3 cut(s)	19, 248, 2556
EarI	CTCTTC	3 cut(s)	19, 248, 2556
EciI	GGCGGA	1 cut(s)	539
Ecl136II	GAGCTC	2 cut(s)	1165, 2084
Eco24I	GRGCYC	3 cut(s)	1108, 1167, 2086
Eco32I	GATATC	1 cut(s)	1540
Eco47III	AGCGCT	1 cut(s)	633
Eco53kI	GAGCTC	2 cut(s)	1165, 2084
Eco57I	CTGAAG	3 cut(s)	242, 1301, 1959
Eco81I	CCTNAGG	1 cut(s)	29
EcoICRI	GAGCTC	2 cut(s)	1165, 2084
EcoO109I	RGGNCCY	2 cut(s)	1104, 1105
EcoRI	GAATTC	1 cut(s)	1804
EcoRII	CCWGG	4 cut(s)	156, 828, 2416, 2700
EcoRV	GATATC	1 cut(s)	1540
EcoT38I	GRGCYC	3 cut(s)	1108, 1167, 2086
FalI	AAGNNNNNCTT	4 cut(s)	1752, 1784, 2629, 2661
FaqI	GGGAC	3 cut(s)	47, 497, 2489
FauNDI	CATATG	1 cut(s)	703
FbaI	TGATCA	3 cut(s)	1189, 1270, 1414
FblI	GTMKAC	1 cut(s)	2150
FokI	GGATG	3 cut(s)	161, 809, 1867
FriOI	GRGCYC	3 cut(s)	1108, 1167, 2086
FspAI	RTGCGCAY	1 cut(s)	2633
FspBI	CTAG	5 cut(s)	689, 801, 2028, 2469, 2538
FspI	TGCGCA	1 cut(s)	2633
GlaI	GCGC	3 cut(s)	26, 633, 2633
GsaI	CCCAGC	2 cut(s)	188, 729
GsuI	CTGGAG	1 cut(s)	2307
HaeII	RGCGCY	1 cut(s)	635
HaeIII	GGCC	4 cut(s)	273, 1106, 1722, 2346
HapII	CCGG	3 cut(s)	18, 469, 2555
HgaI	GACGC	2 cut(s)	222, 2461
HhaI	GCGC	3 cut(s)	27, 634, 2634
Hin6I	GCGC	3 cut(s)	25, 632, 2632
HinP1I	GCGC	3 cut(s)	25, 632, 2632
HindIII	AAGCTT	1 cut(s)	764
HinfI	GANTC	7 cut(s)	4, 40, 343, 848, 967, 2045, 2570
HpaII	CCGG	3 cut(s)	18, 469, 2555
HphI	GGTGA	6 cut(s)	1347, 1475, 2054, 2220, 2316, 2457
Hpy166II	GTNNAC	3 cut(s)	657, 694, 2151
Hpy8I	GTNNAC	3 cut(s)	657, 694, 2151
HpyAV	CCTTC	4 cut(s)	19, 1276, 1980, 2672
HpyCH4III	ACNGT	3 cut(s)	152, 1024, 1516
HpyCH4IV	ACGT	5 cut(s)	52, 79, 928, 1143, 1624
HpySE526I	ACGT	5 cut(s)	52, 79, 928, 1143, 1624
HspAI	GCGC	3 cut(s)	25, 632, 2632
Kpn2I	TCCGGA	2 cut(s)	17, 468
Ksp22I	TGATCA	3 cut(s)	1189, 1270, 1414
LmnI	GCTCC	8 cut(s)	494, 712, 1232, 1984, 2036, 2081, 2089, 2288
MaeI	CTAG	5 cut(s)	689, 801, 2028, 2469, 2538
MaeII	ACGT	5 cut(s)	52, 79, 928, 1143, 1624
MaeIII	GTNAC	8 cut(s)	152, 316, 971, 1139, 1463, 1516, 2463, 2593
MflI	RGATCY	9 cut(s)	337, 457, 685, 736, 1207, 1504, 1573, 1790, 2140
MhlI	GDGCHC	6 cut(s)	491, 659, 1108, 1167, 1399, 2086
MlyI	GAGTC	2 cut(s)	857, 2579
MmeI	TCCRAC	1 cut(s)	346
MroI	TCCGGA	2 cut(s)	17, 468
MroXI	GAANNNNTTC	1 cut(s)	8
MslI	CAYNNNNRTG	3 cut(s)	179, 1007, 1371
MspA1I	CMGCKG	2 cut(s)	1412, 2501
MspCI	CTTAAG	1 cut(s)	1556
MspI	CCGG	3 cut(s)	18, 469, 2555
MspR9I	CCNGG	4 cut(s)	158, 830, 2418, 2702
Mva1269I	GAATGC	1 cut(s)	874
MvaI	CCWGG	4 cut(s)	158, 830, 2418, 2702
MvnI	CGCG	2 cut(s)	25, 233
NdeI	CATATG	1 cut(s)	703
NheI	GCTAGC	1 cut(s)	2537
NlaIV	GGNNCC	4 cut(s)	149, 738, 1106, 1506
NmuCI	GTSAC	4 cut(s)	316, 971, 1463, 2463
NsbI	TGCGCA	1 cut(s)	2633
NspI	RCATGY	4 cut(s)	184, 1006, 1471, 1492
PaeI	GCATGC	1 cut(s)	1492
PasI	CCCWGGG	1 cut(s)	2701
PciI	ACATGT	1 cut(s)	1467
PcsI	WCGNNNNNNNCGW	1 cut(s)	49
PctI	GAATGC	1 cut(s)	874
PdmI	GAANNNNTTC	1 cut(s)	8
PfeI	GAWTC	5 cut(s)	4, 40, 343, 967, 2045
PflMI	CCANNNNNTGG	2 cut(s)	184, 2120
PleI	GAGTC	2 cut(s)	856, 2578
PpsI	GAGTC	2 cut(s)	856, 2578
Ppu21I	YACGTR	2 cut(s)	53, 929
PscI	ACATGT	1 cut(s)	1467
PshBI	ATTAAT	1 cut(s)	537
PsiI	TTATAA	1 cut(s)	503
Psp124BI	GAGCTC	2 cut(s)	1167, 2086
Psp6I	CCWGG	4 cut(s)	156, 828, 2416, 2700
PspFI	CCCAGC	2 cut(s)	184, 725
PspGI	CCWGG	4 cut(s)	156, 828, 2416, 2700
PspN4I	GGNNCC	4 cut(s)	149, 738, 1106, 1506
PspOMI	GGGCCC	1 cut(s)	1104
PspPI	GGNCC	3 cut(s)	1104, 1105, 2345
PstI	CTGCAG	1 cut(s)	70
PstNI	CAGNNNCTG	1 cut(s)	1279
PsuI	RGATCY	9 cut(s)	337, 457, 685, 736, 1207, 1504, 1573, 1790, 2140
PvuII	CAGCTG	2 cut(s)	1412, 2501
RsaI	GTAC	7 cut(s)	604, 716, 1041, 1265, 1311, 1757, 2241
RsaNI	GTAC	7 cut(s)	603, 715, 1040, 1264, 1310, 1756, 2240
RseI	CAYNNNNRTG	3 cut(s)	179, 1007, 1371
SacI	GAGCTC	2 cut(s)	1167, 2086
Sau96I	GGNCC	3 cut(s)	1104, 1105, 2345
SchI	GAGTC	2 cut(s)	857, 2579
ScrFI	CCNGG	4 cut(s)	158, 830, 2418, 2702
SduI	GDGCHC	6 cut(s)	491, 659, 1108, 1167, 1399, 2086
SfcI	CTRYAG	4 cut(s)	66, 142, 2292, 2361
SmiMI	CAYNNNNRTG	3 cut(s)	179, 1007, 1371
SmlI	CTYRAG	2 cut(s)	1032, 1556
SmoI	CTYRAG	2 cut(s)	1032, 1556
SphI	GCATGC	1 cut(s)	1492
SsiI	CCGC	3 cut(s)	524, 1376, 2541
SspI	AATATT	3 cut(s)	394, 946, 1483
SspMI	CTAG	5 cut(s)	689, 801, 2028, 2469, 2538
SstI	GAGCTC	2 cut(s)	1167, 2086
StyD4I	CCNGG	4 cut(s)	156, 828, 2416, 2700
TaaI	ACNGT	3 cut(s)	152, 1024, 1516
TaiI	ACGT	5 cut(s)	55, 82, 931, 1146, 1627
TaqI	TCGA	8 cut(s)	288, 454, 636, 909, 965, 1432, 2064, 2408
TatI	WGTACW	4 cut(s)	602, 1039, 1309, 1755
TauI	GCSGC	1 cut(s)	2544
TfiI	GAWTC	5 cut(s)	4, 40, 343, 967, 2045
TscAI	CASTG	3 cut(s)	628, 1848, 2131
TseFI	GTSAC	4 cut(s)	316, 971, 1463, 2463
Tsp45I	GTSAC	4 cut(s)	316, 971, 1463, 2463
TspGWI	ACGGA	2 cut(s)	749, 2495
TspRI	CASTG	3 cut(s)	628, 1848, 2131
Van91I	CCANNNNNTGG	2 cut(s)	184, 2120
Vha464I	CTTAAG	1 cut(s)	1556
VneI	GTGCAC	1 cut(s)	655
VspI	ATTAAT	1 cut(s)	537
XapI	RAATTY	5 cut(s)	529, 1221, 1303, 1804, 2005
XceI	RCATGY	4 cut(s)	184, 1006, 1471, 1492
XcmI	CCANNNNNNNNNTGG	1 cut(s)	946
XmiI	GTMKAC	1 cut(s)	2150
XmnI	GAANNNNTTC	1 cut(s)	8
XspI	CTAG	5 cut(s)	689, 801, 2028, 2469, 2538

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.