pycom16g10010
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010877
Physical Location & Seq
Forward (+)
6809178 .. 6813506
4329 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2154 bp
ATGGAACCTTCCATATTCTCCGCCCTCATCTTCCTCCTCTTCCTCGTCGACACCACACTCTCGCTCACCCCCGACGGCCTTTCCCTCCTCTCCCTCAAATCCGCCGTCGACCAGTCCTCCGACGGCTCCGCCTTCTCCGACTGGCTCGACACTGATCCCACCCCCTGCCGCTGGACAGGCATTTCCTGCATGAACATCACCGGCTTTTCCGACCCCCGTGTTGTTGGAATCGCTCTCTCCGGAAAGAACCTCCGCGGATACATTCCCTCCGAACTTGGAACATTAGTATACCTTCGCCGCCTTAACCTCCACAGCAACAACTTCCATGGCTCAATCCCTTCCCAACTCTTCAACGCCACCTCACTCCACAGCCTCTTCCTCTATGGTAACAACCTCTCTGGTCCTCTCCCGCCGTCGACATGCAACCCCCCGCGCCTCCAGAACATCGACCTCTCCAACAACTCCCTCTCCGGTCCGCTCCAGAAGGACTACTGGAAGAACTGCCACCAATTACAGCGGCTGATCCTCGCGAGAAACCGATTCGAAGGCGAAATTCCGGCGGGAATTTGGTCTGACATGGAGAATCTAATCCAATTGGACCTCTCCTCCAACGAGTTAACCGGATCGGTGCCGGACGATTTCGGCAAGTTGAAGTCTCTCTCCGGCACGCTCAACTTATCGTTCAATCACTTATCGGGAAAGATTCCGAAATCCCTCGGCGAATTGCCTGTCACGGTGAGCTTTGATCTCCGAAACAATAATTTCAGCGGCGAGATTCCTCAAACCGGTTCGTTTTCGAACCAGGGTCCCACCGCGTTTCTGAACAATCCTCTGCTCTGCGGGTTTCCGCTTCAGAAAGCTTGTAAAAATCCGGGCCAGAGCTCCGCGGATAGTCCGAATTCGGGACCGGGATCGGAAAAAAGTCCGGGAAAGGGATTGAGTCCGGGTCTGATTGTCCTAATCTCGGTGGCGGACGCCGCCGGAGTGGCTTTTATCGGATTAGTCGTCGTTTACATTTACTGGAAAAAGAAGGACGACGAGGACGGATGCAGTTGTACCGGAAAGAGCAATTTTGGCGGCAACGAGAAGCGCCACCTCTGTAATCTCTGCCAGCTCTGTTCTTGCGCTTGCATCAACGGCGGGTTCGGGAACGAGGATTCGGATTCCGGCGACCCGGAAAAGGGAGAGAGGGGGAAAGGGGAGGGGGATCTCGTTGCGATCGACAAGGGTTTCACGTTTGAGCTCGACGAGCTGTTGAGAGCATCGGCTTACGTGCTGGGGAAGAGCGGATTGGGGATTGTGTACAAAGTGGTGCTCGGGAATGGAATCCCGGTGGCGGTGAGGAGGTTGGGGGAGGGCGGTGATCAGCGGTATAAGGAGTTCGCGGCGGAGATTCAGGCGATCGGGCGGGTGAAGCATCCGAATGTGGTGAAGCTGAGGGCGTACTATTGGGCGCCCGACGAGAAGCTTCTCATCAGTGACTTCATTTCTCACGGTAACTTGGCCTCTGCACTTCGCGGGAGAAACGGTCAGTCATCATCAAGCCTCTCGTGGACAACAAGGCTAAAGATCGCCAAGGGAACAGCCCGAGGCCTCGCCTACCTGCACGAATGCAGCCCCAGAAAGTTTGTCCACGGTGACATTAAACCCTCCAACATTCTTCTTGACAACGAATCTCAAGCCTACATTTCAGATTTCGGACTCAACAGACTTATCGAAATCACAGGAAACAACCCCTCCTCGGGTGGCTTCATGGGCGGGGCTTTGCCTTACATGAAACCAGTACAAACAGAGCGAACAAACAATTACCGTGCCCCGGAGGCCCGTGTCACGGGAAGCAAACTCACCCAAAAATGGGACGTGTACTCGTTCGGAGTTGTGTTGCTTGAATTGCTCACAGGGAAGTCCCCGGAGCTCTCTCCGACCACGTCAACTTCGGTCGAGATCCCAGACCTAGTGAGGTGGGTGAGGAAAGGATTTGAGGATGAAAACCCTTTGTCGGATATGGTAGACCCTGTGTTGCTACAGGAAGTGCATGCCAAAAAGGAGGTACTAGCAGCTTTCCATGTAGCCCTTGCATGCACTGAGCCGGACCCTGAGGTTAGGCCAAGGATGAAATCCGTCTCTGAAAATCTTGAAAGGATTGGAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

718

Amino Acids

77.67

Weight (kDa)

6.23

Isoelectric Point (pI)

33.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 63 4.1e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 170 - 231 1.2e-08 Leucine rich repeat
Pkinase PF00069 425 - 651 3.2e-33 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 426 - 712 5.3e-31 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010877)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67510
fragaria_vesca FvH4_4g24010 FvH4_4g24010 FvH4_4g24010
malus_domestica MD13G1116600.v1.1 MD16G1117000.v1.1
prunus_persica Prupe.1G229200_v2.0.a1
pyrus_communis pycom13g10120 pycom16g10010
rosa_chinensis RchiOBHm_Chr4g0430671
rosa_laevigata RLG00000006957
rosa_multiflora Rmu_sc0000131.1_g000012 Rmu_sc0023719.1_g000001
rosa_roxburghii Rroxscaffold_5G00372410
rosa_rugosa Rorug04G0245100
rosa_samantha Rh4AG301200 Rh4BG308300 Rh4CG325500 Rh4DG305900
rosa_wichuraiana Rw4G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1611
AccB1I GGYRCC 2 cut(s) 628, 1453
AccBSI CCGCTC 2 cut(s) 478, 1287
AccI GTMKAC 5 cut(s) 48, 108, 288, 416, 2010
AccII CGCG 7 cut(s) 255, 433, 530, 815, 887, 1385, 1518
AccIII TCCGGA 1 cut(s) 239
AclWI GGATC 6 cut(s) 149, 517, 631, 919, 1215, 1939
AcsI RAATTY 3 cut(s) 552, 564, 898
AcuI CTGAAG 1 cut(s) 836
AcyI GRCGYC 2 cut(s) 975, 1454
AfaI GTAC 6 cut(s) 1057, 1304, 1445, 1785, 1865, 2052
AflIII ACRYGT 1 cut(s) 1860
AgeI ACCGGT 1 cut(s) 785
AgsI TTSAA 5 cut(s) 352, 652, 685, 1889, 2138
AjiI CACGTC 2 cut(s) 1861, 1929
AjnI CCWGG 1 cut(s) 801
AjuI GAANNNNNNNTTGG 2 cut(s) 1274, 1306
Alw21I GWGCWC 4 cut(s) 884, 1245, 1317, 1917
Alw26I GTCTC 2 cut(s) 660, 2128
AlwI GGATC 6 cut(s) 149, 517, 631, 919, 1215, 1939
AlwNI CAGNNNCTG 1 cut(s) 520
Ama87I CYCGRG 3 cut(s) 1316, 1587, 1741
Aor13HI TCCGGA 1 cut(s) 239
AoxI GGCC 6 cut(s) 76, 874, 1503, 1591, 1821, 2105
ApeKI GCWGC 2 cut(s) 1614, 2057
ApoI RAATTY 3 cut(s) 552, 564, 898
ArsI GACNNNNNNTTYG 4 cut(s) 90, 122, 714, 746
AsiGI ACCGGT 1 cut(s) 785
AspLEI GCGC 4 cut(s) 435, 1092, 1127, 1456
AspS9I GGNCC 8 cut(s) 401, 473, 598, 806, 874, 905, 1822, 2092
AsuC2I CCSGG 8 cut(s) 873, 909, 927, 945, 1175, 1331, 1817, 1910
AsuII TTCGAA 2 cut(s) 543, 797
AvaI CYCGRG 3 cut(s) 1316, 1587, 1741
AvaII GGWCC 6 cut(s) 401, 473, 598, 806, 905, 2092
AxyI CCTNAGG 1 cut(s) 2097
BaeGI GKGCMC 1 cut(s) 1816
BanI GGYRCC 2 cut(s) 628, 1453
BanII GRGCYC 3 cut(s) 884, 1245, 1917
BauI CACGAG 1 cut(s) 1549
Bbv12I GWGCWC 4 cut(s) 884, 1245, 1317, 1917
BbvCI CCTCAGC 1 cut(s) 1436
BbvI GCAGC 2 cut(s) 1626, 2069
BceAI ACGGC 5 cut(s) 89, 91, 139, 397, 1153
BciT130I CCWGG 1 cut(s) 803
BciVI GTATCC 1 cut(s) 251
BclI TGATCA 1 cut(s) 1363
BcnI CCSGG 8 cut(s) 873, 909, 927, 945, 1175, 1331, 1817, 1910
BcoDI GTCTC 2 cut(s) 660, 2128
BfaI CTAG 2 cut(s) 1955, 2054
BfmI CTRYAG 1 cut(s) 2024
BfoI RGCGCY 2 cut(s) 1093, 1457
BfuAI ACCTGC 1 cut(s) 1611
BfuI GTATCC 1 cut(s) 251
BglI GCCNNNNNGGC 2 cut(s) 986, 1820
BisI GCNGC 9 cut(s) 169, 298, 518, 769, 978, 1078, 1386, 1615, 2058
BlsI GCNGC 9 cut(s) 170, 299, 519, 770, 979, 1079, 1387, 1616, 2059
Bme1390I CCNGG 9 cut(s) 803, 873, 909, 927, 945, 1175, 1331, 1817, 1910
Bme18I GGWCC 6 cut(s) 401, 473, 598, 806, 905, 2092
BmeT110I CYCGRG 3 cut(s) 1316, 1587, 1741
BmgBI CACGTC 2 cut(s) 1861, 1929
BmgT120I GGNCC 8 cut(s) 401, 473, 598, 806, 874, 905, 1822, 2092
BmiI GGNNCC 8 cut(s) 6, 127, 630, 807, 808, 906, 1455, 2094
BmrFI CCNGG 9 cut(s) 803, 873, 909, 927, 945, 1175, 1331, 1817, 1910
BmsI GCATC 4 cut(s) 1037, 1140, 1271, 1426
BpmI CTGGAG 2 cut(s) 422, 464
Bpu10I CCTNAGC 1 cut(s) 1436
Bpu14I TTCGAA 2 cut(s) 543, 797
BpuEI CTTGAG 1 cut(s) 1662
BpuMI CCSGG 8 cut(s) 873, 909, 927, 945, 1175, 1331, 1817, 1910
BsaAI YACGTR 1 cut(s) 1273
BsaHI GRCGYC 2 cut(s) 975, 1454
BsaWI WCCGGW 5 cut(s) 239, 470, 620, 785, 1058
Bse118I RCCGGY 2 cut(s) 200, 785
Bse1I ACTGG 5 cut(s) 112, 146, 497, 1025, 1781
Bse21I CCTNAGG 1 cut(s) 2097
BseAI TCCGGA 1 cut(s) 239
BseBI CCWGG 1 cut(s) 803
BseGI GGATG 4 cut(s) 1052, 1417, 1990, 2118
BseMII CTCAG 3 cut(s) 1427, 2076, 2088
BseNI ACTGG 5 cut(s) 112, 146, 497, 1025, 1781
BseRI GAGGAG 5 cut(s) 26, 77, 595, 1357, 1729
BseSI GKGCMC 1 cut(s) 1816
BseXI GCAGC 2 cut(s) 1626, 2069
BseYI CCCAGC 1 cut(s) 1276
BsgI GTGCAG 2 cut(s) 1494, 1589
Bsh1236I CGCG 7 cut(s) 255, 433, 530, 815, 887, 1385, 1518
Bsh1285I CGRYCG 3 cut(s) 1221, 1404, 1941
BshFI GGCC 6 cut(s) 78, 876, 1505, 1593, 1823, 2107
BshNI GGYRCC 2 cut(s) 628, 1453
BshTI ACCGGT 1 cut(s) 785
BsiEI CGRYCG 3 cut(s) 1221, 1404, 1941
BsiHKAI GWGCWC 4 cut(s) 884, 1245, 1317, 1917
BsiHKCI CYCGRG 3 cut(s) 1316, 1587, 1741
BslFI GGGAC 4 cut(s) 792, 918, 1871, 1891
BsmAI GTCTC 2 cut(s) 660, 2128
BsmBI CGTCTC 1 cut(s) 2128
BsmFI GGGAC 4 cut(s) 792, 918, 1871, 1891
BsmI GAATGC 1 cut(s) 1616
BsnI GGCC 6 cut(s) 78, 876, 1505, 1593, 1823, 2107
BsoBI CYCGRG 3 cut(s) 1316, 1587, 1741
Bsp119I TTCGAA 2 cut(s) 543, 797
Bsp1286I GDGCHC 5 cut(s) 884, 1245, 1317, 1816, 1917
Bsp13I TCCGGA 1 cut(s) 239
Bsp1407I TGTACA 1 cut(s) 1302
Bsp19I CCATGG 1 cut(s) 325
Bsp68I TCGCGA 1 cut(s) 530
BspANI GGCC 6 cut(s) 78, 876, 1505, 1593, 1823, 2107
BspCNI CTCAG 3 cut(s) 1428, 2077, 2089
BspEI TCCGGA 1 cut(s) 239
BspFNI CGCG 7 cut(s) 255, 433, 530, 815, 887, 1385, 1518
BspLI GGNNCC 8 cut(s) 6, 127, 630, 807, 808, 906, 1455, 2094
BspMI ACCTGC 1 cut(s) 1611
BspPI GGATC 6 cut(s) 149, 517, 631, 919, 1215, 1939
BspQI GCTCTTC 1 cut(s) 1277
BspT104I TTCGAA 2 cut(s) 543, 797
BspT107I GGYRCC 2 cut(s) 628, 1453
BsrBI CCGCTC 2 cut(s) 478, 1287
BsrFI RCCGGY 2 cut(s) 200, 785
BsrGI TGTACA 1 cut(s) 1302
BsrI ACTGG 5 cut(s) 112, 146, 497, 1025, 1781
BssAI RCCGGY 2 cut(s) 200, 785
BssNAI GTATAC 1 cut(s) 289
BssNI GRCGYC 2 cut(s) 975, 1454
BssSI CACGAG 1 cut(s) 1549
BssT1I CCWWGG 3 cut(s) 325, 1575, 2108
Bst1107I GTATAC 1 cut(s) 289
Bst2BI CACGAG 1 cut(s) 1549
Bst2UI CCWGG 1 cut(s) 803
Bst4CI ACNGT 5 cut(s) 736, 1496, 1529, 1637, 1811
Bst6I CTCTTC 4 cut(s) 44, 353, 380, 1277
BstACI GRCGYC 2 cut(s) 975, 1454
BstAPI GCANNNNNTGC 1 cut(s) 186
BstAUI TGTACA 1 cut(s) 1302
BstBAI YACGTR 1 cut(s) 1273
BstBI TTCGAA 2 cut(s) 543, 797
BstC8I GCNNGC 5 cut(s) 668, 1112, 1129, 2037, 2080
BstDEI CTNAG 3 cut(s) 1436, 2085, 2097
BstDSI CCRYGG 4 cut(s) 253, 325, 885, 1633
BstF5I GGATG 4 cut(s) 1052, 1417, 1990, 2118
BstFNI CGCG 7 cut(s) 255, 433, 530, 815, 887, 1385, 1518
BstH2I RGCGCY 2 cut(s) 1093, 1457
BstHHI GCGC 4 cut(s) 435, 1092, 1127, 1456
BstMAI GTCTC 2 cut(s) 660, 2128
BstMCI CGRYCG 3 cut(s) 1221, 1404, 1941
BstNI CCWGG 1 cut(s) 803
BstNSI RCATGY 3 cut(s) 423, 2039, 2082
BstSCI CCNGG 9 cut(s) 801, 871, 907, 925, 943, 1173, 1329, 1815, 1908
BstSFI CTRYAG 1 cut(s) 2024
BstSLI GKGCMC 1 cut(s) 1816
BstUI CGCG 7 cut(s) 255, 433, 530, 815, 887, 1385, 1518
BstV1I GCAGC 2 cut(s) 1626, 2069
BstX2I RGATCY 2 cut(s) 1207, 1944
BstYI RGATCY 2 cut(s) 1207, 1944
BstZ17I GTATAC 1 cut(s) 289
Bsu36I CCTNAGG 1 cut(s) 2097
BsuI GTATCC 1 cut(s) 251
BsuRI GGCC 6 cut(s) 78, 876, 1505, 1593, 1823, 2107
BtgI CCRYGG 4 cut(s) 253, 325, 885, 1633
BtrI CACGTC 2 cut(s) 1861, 1929
BtsCI GGATG 4 cut(s) 1052, 1417, 1990, 2118
BtsIMutI CAGTG 3 cut(s) 150, 1483, 2082
BtuMI TCGCGA 1 cut(s) 530
BveI ACCTGC 1 cut(s) 1611
Cac8I GCNNGC 5 cut(s) 668, 1112, 1129, 2037, 2080
CaiI CAGNNNCTG 1 cut(s) 520
CfoI GCGC 4 cut(s) 435, 1092, 1127, 1456
Cfr10I RCCGGY 2 cut(s) 200, 785
Cfr13I GGNCC 8 cut(s) 401, 473, 598, 806, 874, 905, 1822, 2092
Cfr42I CCGCGG 2 cut(s) 256, 888
CpoI CGGWCCG 1 cut(s) 473
CseI GACGC 1 cut(s) 983
Csp6I GTAC 6 cut(s) 1056, 1303, 1444, 1784, 1864, 2051
CspAI ACCGGT 1 cut(s) 785
CspI CGGWCCG 1 cut(s) 473
CviAII CATG 9 cut(s) 190, 326, 420, 577, 1753, 1774, 2036, 2066, 2079
CviQI GTAC 6 cut(s) 1056, 1303, 1444, 1784, 1864, 2051
DdeI CTNAG 3 cut(s) 1436, 2085, 2097
DinI GGCGCC 1 cut(s) 1455
Eam1104I CTCTTC 4 cut(s) 44, 353, 380, 1277
EarI CTCTTC 4 cut(s) 44, 353, 380, 1277
EciI GGCGGA 5 cut(s) 10, 91, 118, 986, 1403
Ecl136II GAGCTC 3 cut(s) 882, 1243, 1915
Eco130I CCWWGG 3 cut(s) 325, 1575, 2108
Eco147I AGGCCT 1 cut(s) 1593
Eco24I GRGCYC 3 cut(s) 884, 1245, 1917
Eco47I GGWCC 6 cut(s) 401, 473, 598, 806, 905, 2092
Eco53kI GAGCTC 3 cut(s) 882, 1243, 1915
Eco57I CTGAAG 1 cut(s) 836
Eco81I CCTNAGG 1 cut(s) 2097
Eco88I CYCGRG 3 cut(s) 1316, 1587, 1741
EcoICRI GAGCTC 3 cut(s) 882, 1243, 1915
EcoO109I RGGNCCY 1 cut(s) 806
EcoRI GAATTC 1 cut(s) 898
EcoRII CCWGG 1 cut(s) 801
EcoT14I CCWWGG 3 cut(s) 325, 1575, 2108
EcoT38I GRGCYC 3 cut(s) 884, 1245, 1917
EgeI GGCGCC 1 cut(s) 1455
EheI GGCGCC 1 cut(s) 1455
ErhI CCWWGG 3 cut(s) 325, 1575, 2108
Esp3I CGTCTC 1 cut(s) 2128
FaeI CATG 9 cut(s) 193, 329, 423, 580, 1756, 1777, 2039, 2069, 2082
FaqI GGGAC 4 cut(s) 792, 918, 1871, 1891
FatI CATG 9 cut(s) 189, 325, 419, 576, 1752, 1773, 2035, 2065, 2078
FauI CCCGC 8 cut(s) 417, 438, 553, 833, 1133, 1401, 1511, 1751
FbaI TGATCA 1 cut(s) 1363
FblI GTMKAC 5 cut(s) 48, 108, 288, 416, 2010
Fnu4HI GCNGC 9 cut(s) 169, 298, 518, 769, 978, 1078, 1386, 1615, 2058
FokI GGATG 4 cut(s) 1059, 1404, 1997, 2125
FriOI GRGCYC 3 cut(s) 884, 1245, 1917
Fsp4HI GCNGC 9 cut(s) 169, 298, 518, 769, 978, 1078, 1386, 1615, 2058
FspBI CTAG 2 cut(s) 1955, 2054
GlaI GCGC 4 cut(s) 434, 1091, 1126, 1455
GluI GCNGC 9 cut(s) 169, 298, 518, 769, 978, 1078, 1386, 1615, 2058
GsaI CCCAGC 1 cut(s) 1280
GsuI CTGGAG 2 cut(s) 422, 464
HaeII RGCGCY 2 cut(s) 1093, 1457
HaeIII GGCC 6 cut(s) 78, 876, 1505, 1593, 1823, 2107
HgaI GACGC 1 cut(s) 983
HhaI GCGC 4 cut(s) 435, 1092, 1127, 1456
Hin1I GRCGYC 2 cut(s) 975, 1454
Hin1II CATG 9 cut(s) 193, 329, 423, 580, 1756, 1777, 2039, 2069, 2082
Hin6I GCGC 4 cut(s) 433, 1090, 1125, 1454
HinP1I GCGC 4 cut(s) 433, 1090, 1125, 1454
HincII GTYRAC 5 cut(s) 49, 109, 417, 618, 1932
HindII GTYRAC 5 cut(s) 49, 109, 417, 618, 1932
HindIII AAGCTT 2 cut(s) 858, 1466
HpaI GTTAAC 1 cut(s) 618
Hpy99I CGWCG 9 cut(s) 50, 77, 110, 125, 418, 1010, 1040, 1250, 1463
HpyAV CCTTC 7 cut(s) 18, 142, 302, 348, 478, 539, 1024
HpyCH4III ACNGT 5 cut(s) 736, 1496, 1529, 1637, 1811
HpyCH4IV ACGT 5 cut(s) 1235, 1272, 1860, 1928, 2150
HpyF3I CTNAG 3 cut(s) 1436, 2085, 2097
HpySE526I ACGT 5 cut(s) 1235, 1272, 1860, 1928, 2150
Hsp92I GRCGYC 2 cut(s) 975, 1454
Hsp92II CATG 9 cut(s) 193, 329, 423, 580, 1756, 1777, 2039, 2069, 2082
HspAI GCGC 4 cut(s) 433, 1090, 1125, 1454
KasI GGCGCC 1 cut(s) 1453
KflI GGGWCCC 1 cut(s) 806
Kpn2I TCCGGA 1 cut(s) 239
Ksp22I TGATCA 1 cut(s) 1363
KspAI GTTAAC 1 cut(s) 618
KspI CCGCGG 2 cut(s) 256, 888
LguI GCTCTTC 1 cut(s) 1277
LmnI GCTCC 4 cut(s) 131, 483, 887, 1912
Lsp1109I GCAGC 2 cut(s) 1626, 2069
LweI GCATC 4 cut(s) 1037, 1140, 1271, 1426
MaeI CTAG 2 cut(s) 1955, 2054
MaeII ACGT 5 cut(s) 1235, 1272, 1860, 1928, 2150
MaeIII GTNAC 6 cut(s) 386, 730, 1478, 1496, 1637, 1828
MbiI CCGCTC 2 cut(s) 478, 1287
MboII GAAGA 7 cut(s) 22, 31, 340, 367, 508, 1294, 1652
MfeI CAATTG 1 cut(s) 593
MflI RGATCY 2 cut(s) 1207, 1944
MhlI GDGCHC 5 cut(s) 884, 1245, 1317, 1816, 1917
Mly113I GGCGCC 1 cut(s) 1454
MlyI GAGTC 2 cut(s) 949, 1695
MmeI TCCRAC 9 cut(s) 144, 162, 205, 234, 480, 633, 1677, 1946, 1980
MroI TCCGGA 1 cut(s) 239
MseI TTAA 3 cut(s) 303, 617, 1644
MslI CAYNNNNRTG 1 cut(s) 1422
MspA1I CMGCKG 6 cut(s) 171, 255, 517, 768, 887, 1369
MspR9I CCNGG 9 cut(s) 803, 873, 909, 927, 945, 1175, 1331, 1817, 1910
MunI CAATTG 1 cut(s) 593
Mva1269I GAATGC 1 cut(s) 1616
MvaI CCWGG 1 cut(s) 803
MvnI CGCG 7 cut(s) 255, 433, 530, 815, 887, 1385, 1518
NarI GGCGCC 1 cut(s) 1454
NciI CCSGG 8 cut(s) 873, 909, 927, 945, 1175, 1331, 1817, 1910
NcoI CCATGG 1 cut(s) 325
NlaIII CATG 9 cut(s) 193, 329, 423, 580, 1756, 1777, 2039, 2069, 2082
NlaIV GGNNCC 8 cut(s) 6, 127, 630, 807, 808, 906, 1455, 2094
NmeAIII GCCGAG 1 cut(s) 696
NmuCI GTSAC 4 cut(s) 730, 1478, 1637, 1828
NruI TCGCGA 1 cut(s) 530
NspI RCATGY 3 cut(s) 423, 2039, 2082
NspV TTCGAA 2 cut(s) 543, 797
PaeI GCATGC 2 cut(s) 2039, 2082
PceI AGGCCT 1 cut(s) 1593
PciSI GCTCTTC 1 cut(s) 1277
PcsI WCGNNNNNNNCGW 2 cut(s) 1002, 1218
PctI GAATGC 1 cut(s) 1616
PflFI GACNNNGTC 1 cut(s) 1927
PfoI TCCNGGA 1 cut(s) 925
PinAI ACCGGT 1 cut(s) 785
PkrI GCNGC 9 cut(s) 170, 299, 519, 770, 979, 1079, 1387, 1616, 2059
Ple19I CGATCG 2 cut(s) 1221, 1404
PleI GAGTC 2 cut(s) 948, 1695
PluTI GGCGCC 1 cut(s) 1457
PpsI GAGTC 2 cut(s) 948, 1695
Ppu21I YACGTR 1 cut(s) 1273
PpuMI RGGWCCY 1 cut(s) 806
Psp124BI GAGCTC 3 cut(s) 884, 1245, 1917
Psp5II RGGWCCY 1 cut(s) 806
Psp6I CCWGG 1 cut(s) 801
PspFI CCCAGC 1 cut(s) 1276
PspGI CCWGG 1 cut(s) 801
PspN4I GGNNCC 8 cut(s) 6, 127, 630, 807, 808, 906, 1455, 2094
PspPI GGNCC 8 cut(s) 401, 473, 598, 806, 874, 905, 1822, 2092
PspPPI RGGWCCY 1 cut(s) 806
PsrI GAACNNNNNNTAC 2 cut(s) 1364, 1396
PstNI CAGNNNCTG 1 cut(s) 520
PsuI RGATCY 2 cut(s) 1207, 1944
PsyI GACNNNGTC 1 cut(s) 1927
PvuI CGATCG 2 cut(s) 1221, 1404
RruI TCGCGA 1 cut(s) 530
RsaI GTAC 6 cut(s) 1057, 1304, 1445, 1785, 1865, 2052
RsaNI GTAC 6 cut(s) 1056, 1303, 1444, 1784, 1864, 2051
RseI CAYNNNNRTG 1 cut(s) 1422
Rsr2I CGGWCCG 1 cut(s) 473
RsrII CGGWCCG 1 cut(s) 473
SacI GAGCTC 3 cut(s) 884, 1245, 1917
SacII CCGCGG 2 cut(s) 256, 888
SalI GTCGAC 3 cut(s) 47, 107, 415
SapI GCTCTTC 1 cut(s) 1277
SaqAI TTAA 3 cut(s) 303, 617, 1644
SatI GCNGC 9 cut(s) 169, 298, 518, 769, 978, 1078, 1386, 1615, 2058
Sau96I GGNCC 8 cut(s) 401, 473, 598, 806, 874, 905, 1822, 2092
SchI GAGTC 2 cut(s) 949, 1695
ScrFI CCNGG 9 cut(s) 803, 873, 909, 927, 945, 1175, 1331, 1817, 1910
SduI GDGCHC 5 cut(s) 884, 1245, 1317, 1816, 1917
SfaNI GCATC 4 cut(s) 1037, 1140, 1271, 1426
SfcI CTRYAG 1 cut(s) 2024
SfoI GGCGCC 1 cut(s) 1455
Sfr303I CCGCGG 2 cut(s) 256, 888
SfuI TTCGAA 2 cut(s) 543, 797
SgrBI CCGCGG 2 cut(s) 256, 888
SinI GGWCC 6 cut(s) 401, 473, 598, 806, 905, 2092
SmiMI CAYNNNNRTG 1 cut(s) 1422
SmlI CTYRAG 1 cut(s) 1677
SmoI CTYRAG 1 cut(s) 1677
SphI GCATGC 2 cut(s) 2039, 2082
SseBI AGGCCT 1 cut(s) 1593
SspDI GGCGCC 1 cut(s) 1453
SspMI CTAG 2 cut(s) 1955, 2054
SstI GAGCTC 3 cut(s) 884, 1245, 1917
StuI AGGCCT 1 cut(s) 1593
StyD4I CCNGG 9 cut(s) 801, 871, 907, 925, 943, 1173, 1329, 1815, 1908
StyI CCWWGG 3 cut(s) 325, 1575, 2108
TaaI ACNGT 5 cut(s) 736, 1496, 1529, 1637, 1811
TaiI ACGT 5 cut(s) 1238, 1275, 1863, 1931, 2153
TaqII GACCGA 1 cut(s) 1927
TatI WGTACW 3 cut(s) 1302, 1783, 1863
TauI GCSGC 7 cut(s) 171, 300, 520, 771, 980, 1080, 1388
Tru1I TTAA 3 cut(s) 303, 617, 1644
Tru9I TTAA 3 cut(s) 303, 617, 1644
TscAI CASTG 3 cut(s) 157, 1483, 2089
TseFI GTSAC 4 cut(s) 730, 1478, 1637, 1828
TseI GCWGC 2 cut(s) 1614, 2057
Tsp45I GTSAC 4 cut(s) 730, 1478, 1637, 1828
TspDTI ATGAA 6 cut(s) 206, 1474, 1741, 1790, 2001, 2129
TspGWI ACGGA 2 cut(s) 1059, 2110
TspRI CASTG 3 cut(s) 157, 1483, 2089
Tth111I GACNNNGTC 1 cut(s) 1927
VpaK11BI GGWCC 6 cut(s) 401, 473, 598, 806, 905, 2092
XapI RAATTY 3 cut(s) 552, 564, 898
XceI RCATGY 3 cut(s) 423, 2039, 2082
XmiI GTMKAC 5 cut(s) 48, 108, 288, 416, 2010
XspI CTAG 2 cut(s) 1955, 2054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.