pycom16g11590

50S ribosomal protein L18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009178
Physical Location & Seq
Reverse (-)
8068277 .. 8069910
1634 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
ATGTCAGCTTCGCTTTCGTTCCTAGCGTCTTCTCAGCAGCTATGGCTGCGTCCTCAGGTCGTGGGAGTGGCGGCGACTCCGTCTCCAAGGCCTCAGTCTCGGTCTCTTGTCGTGGAAGCCAAAGCCACCACCAAAAGAGAAGACAGGACCGCCCGCCATACCCGTATCAGAAAGAAGGTTGAAGGGACTCCCGAAAGGCCCAGGCTGGCTGTCTTCCGCTCCCACAAGCATATCTATGTCCAAGTGATTGATGATAGCAAGATGCACACACTTGCTTCGGCTTCAACGATGCAGAAACCCCTCTCCGAAGAATTTGACTACACTTCTGGTCCTACCATTGAAGTAGCAAAGAAGGTAGGAGAAGTCATTGCAAAGTCATGTTTGGAGAAAGGGATCTCAAAGGTGGCCTTTGACCGTGGTGGATACCCATATCATGGACGTGTGCAAGCCCTTGCTGATGCAGCCAGGGAACACGGCCTTCAGTTCTAA

Protein Analysis

163

Amino Acids

17.96

Weight (kDa)

10.0

Isoelectric Point (pI)

56.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 44 - 162 2.1e-46 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 434
AccBSI CCGCTC 1 cut(s) 219
AciI CCGC 4 cut(s) 71, 150, 154, 217
AclWI GGATC 1 cut(s) 401
AcsI RAATTY 1 cut(s) 311
AcuI CTGAAG 1 cut(s) 464
AfiI CCNNNNNNNGG 1 cut(s) 434
AflIII ACRYGT 1 cut(s) 439
AgsI TTSAA 3 cut(s) 182, 285, 341
AjiI CACGTC 1 cut(s) 440
AjnI CCWGG 2 cut(s) 200, 464
AluBI AGCT 2 cut(s) 8, 40
AluI AGCT 2 cut(s) 8, 40
Alw26I GTCTC 3 cut(s) 87, 102, 108
AlwI GGATC 1 cut(s) 401
AoxI GGCC 4 cut(s) 89, 197, 405, 475
ApeKI GCWGC 3 cut(s) 37, 46, 461
ApoI RAATTY 1 cut(s) 311
AspS9I GGNCC 3 cut(s) 147, 198, 329
AvaII GGWCC 2 cut(s) 147, 329
AxyI CCTNAGG 1 cut(s) 54
BbsI GAAGAC 3 cut(s) 21, 147, 205
BbvI GCAGC 3 cut(s) 33, 49, 473
BciT130I CCWGG 2 cut(s) 202, 466
BciVI GTATCC 1 cut(s) 416
BcoDI GTCTC 3 cut(s) 87, 102, 108
BfaI CTAG 1 cut(s) 23
BfuI GTATCC 1 cut(s) 416
BisI GCNGC 4 cut(s) 38, 47, 72, 462
BlsI GCNGC 4 cut(s) 39, 48, 73, 463
Bme1390I CCNGG 2 cut(s) 202, 466
Bme18I GGWCC 2 cut(s) 147, 329
BmgBI CACGTC 1 cut(s) 440
BmgT120I GGNCC 3 cut(s) 147, 198, 329
BmrFI CCNGG 2 cut(s) 202, 466
BmsI GCATC 3 cut(s) 252, 279, 448
BpiI GAAGAC 3 cut(s) 21, 147, 205
BsaI GGTCTC 1 cut(s) 108
BsaJI CCNNGG 4 cut(s) 86, 200, 415, 465
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 1 cut(s) 434
Bse21I CCTNAGG 1 cut(s) 54
Bse3DI GCAATG 1 cut(s) 366
BseBI CCWGG 2 cut(s) 202, 466
BseDI CCNNGG 4 cut(s) 86, 200, 415, 465
BseLI CCNNNNNNNGG 1 cut(s) 434
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 3 cut(s) 47, 68, 107
BseXI GCAGC 3 cut(s) 33, 49, 473
BshFI GGCC 4 cut(s) 91, 199, 407, 477
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 1 cut(s) 434
BsmAI GTCTC 3 cut(s) 87, 102, 108
BsmBI CGTCTC 1 cut(s) 87
BsmFI GGGAC 1 cut(s) 199
BsnI GGCC 4 cut(s) 91, 199, 407, 477
Bso31I GGTCTC 1 cut(s) 108
Bsp143I GATC 1 cut(s) 393
BspACI CCGC 4 cut(s) 71, 150, 154, 217
BspANI GGCC 4 cut(s) 91, 199, 407, 477
BspCNI CTCAG 3 cut(s) 46, 67, 106
BspPI GGATC 1 cut(s) 401
BspTNI GGTCTC 1 cut(s) 108
BsrBI CCGCTC 1 cut(s) 219
BsrDI GCAATG 1 cut(s) 366
BssECI CCNNGG 4 cut(s) 86, 200, 415, 465
BssMI GATC 1 cut(s) 393
BssT1I CCWWGG 1 cut(s) 86
Bst2UI CCWGG 2 cut(s) 202, 466
Bst4CI ACNGT 1 cut(s) 416
BstC8I GCNNGC 3 cut(s) 154, 207, 447
BstDEI CTNAG 3 cut(s) 33, 54, 93
BstDSI CCRYGG 1 cut(s) 415
BstKTI GATC 1 cut(s) 396
BstMAI GTCTC 3 cut(s) 87, 102, 108
BstMBI GATC 1 cut(s) 393
BstMWI GCNNNNNNNGC 3 cut(s) 43, 46, 461
BstNI CCWGG 2 cut(s) 202, 466
BstSCI CCNGG 2 cut(s) 200, 464
BstV1I GCAGC 3 cut(s) 33, 49, 473
BstV2I GAAGAC 3 cut(s) 21, 147, 205
BstX2I RGATCY 1 cut(s) 393
BstYI RGATCY 1 cut(s) 393
Bsu36I CCTNAGG 1 cut(s) 54
BsuI GTATCC 1 cut(s) 416
BsuRI GGCC 4 cut(s) 91, 199, 407, 477
BtgI CCRYGG 1 cut(s) 415
BtrI CACGTC 1 cut(s) 440
Cac8I GCNNGC 3 cut(s) 154, 207, 447
Cfr13I GGNCC 3 cut(s) 147, 198, 329
CseI GACGC 2 cut(s) 15, 38
CviAII CATG 2 cut(s) 378, 434
DdeI CTNAG 3 cut(s) 33, 54, 93
DpnI GATC 1 cut(s) 395
DpnII GATC 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 86
Eco147I AGGCCT 1 cut(s) 91
Eco31I GGTCTC 1 cut(s) 108
Eco47I GGWCC 2 cut(s) 147, 329
Eco57I CTGAAG 1 cut(s) 464
Eco81I CCTNAGG 1 cut(s) 54
EcoRII CCWGG 2 cut(s) 200, 464
EcoT14I CCWWGG 1 cut(s) 86
ErhI CCWWGG 1 cut(s) 86
Esp3I CGTCTC 1 cut(s) 87
FaeI CATG 2 cut(s) 381, 437
FaiI YATR 7 cut(s) 43, 159, 231, 237, 379, 430, 435
FalI AAGNNNNNCTT 2 cut(s) 392, 424
FaqI GGGAC 1 cut(s) 199
FatI CATG 2 cut(s) 377, 433
FauI CCCGC 1 cut(s) 161
Fnu4HI GCNGC 4 cut(s) 38, 47, 72, 462
Fsp4HI GCNGC 4 cut(s) 38, 47, 72, 462
FspBI CTAG 1 cut(s) 23
GluI GCNGC 4 cut(s) 38, 47, 72, 462
HaeIII GGCC 4 cut(s) 91, 199, 407, 477
HgaI GACGC 2 cut(s) 15, 38
Hin1II CATG 2 cut(s) 381, 437
HinfI GANTC 2 cut(s) 76, 187
Hpy188I TCNGA 2 cut(s) 170, 307
Hpy188III TCNNGA 1 cut(s) 191
HpyAV CCTTC 4 cut(s) 169, 176, 346, 488
HpyCH4III ACNGT 1 cut(s) 416
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 5 cut(s) 265, 292, 371, 445, 461
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 46, 461
HpyF3I CTNAG 3 cut(s) 33, 54, 93
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 2 cut(s) 381, 437
Kzo9I GATC 1 cut(s) 393
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 8 cut(s) 41, 130, 187, 191, 214, 312, 451, 478
Lsp1109I GCAGC 3 cut(s) 33, 49, 473
LweI GCATC 3 cut(s) 252, 279, 448
MaeI CTAG 1 cut(s) 23
MaeII ACGT 1 cut(s) 439
MalI GATC 1 cut(s) 395
MbiI CCGCTC 1 cut(s) 219
MboI GATC 1 cut(s) 393
MboII GAAGA 4 cut(s) 21, 152, 205, 320
MflI RGATCY 1 cut(s) 393
MluCI AATT 1 cut(s) 311
MlyI GAGTC 2 cut(s) 70, 181
MnlI CCTC 3 cut(s) 63, 102, 311
MslI CAYNNNNRTG 2 cut(s) 234, 438
MspR9I CCNGG 2 cut(s) 202, 466
MvaI CCWGG 2 cut(s) 202, 466
MwoI GCNNNNNNNGC 3 cut(s) 43, 46, 461
NdeII GATC 1 cut(s) 393
NlaIII CATG 2 cut(s) 381, 437
PceI AGGCCT 1 cut(s) 91
PcsI WCGNNNNNNNCGW 2 cut(s) 23, 284
PflFI GACNNNGTC 1 cut(s) 79
PflMI CCANNNNNTGG 1 cut(s) 434
PkrI GCNGC 4 cut(s) 39, 48, 73, 463
PleI GAGTC 2 cut(s) 70, 181
PpsI GAGTC 2 cut(s) 70, 181
Psp6I CCWGG 2 cut(s) 200, 464
PspGI CCWGG 2 cut(s) 200, 464
PspPI GGNCC 3 cut(s) 147, 198, 329
PsuI RGATCY 1 cut(s) 393
PsyI GACNNNGTC 1 cut(s) 79
RseI CAYNNNNRTG 2 cut(s) 234, 438
SatI GCNGC 4 cut(s) 38, 47, 72, 462
Sau3AI GATC 1 cut(s) 393
Sau96I GGNCC 3 cut(s) 147, 198, 329
SchI GAGTC 2 cut(s) 70, 181
ScrFI CCNGG 2 cut(s) 202, 466
SetI ASST 7 cut(s) 10, 42, 60, 180, 357, 405, 442
SfaNI GCATC 3 cut(s) 252, 279, 448
SinI GGWCC 2 cut(s) 147, 329
SmiMI CAYNNNNRTG 2 cut(s) 234, 438
Sse9I AATT 1 cut(s) 311
SseBI AGGCCT 1 cut(s) 91
SsiI CCGC 4 cut(s) 71, 150, 154, 217
SspMI CTAG 1 cut(s) 23
StuI AGGCCT 1 cut(s) 91
StyD4I CCNGG 2 cut(s) 200, 464
StyI CCWWGG 1 cut(s) 86
TaaI ACNGT 1 cut(s) 416
TaiI ACGT 1 cut(s) 442
TaqII GACCGA 1 cut(s) 90
TasI AATT 1 cut(s) 311
TauI GCSGC 1 cut(s) 74
TseI GCWGC 3 cut(s) 37, 46, 461
TspGWI ACGGA 1 cut(s) 69
Tth111I GACNNNGTC 1 cut(s) 79
Van91I CCANNNNNTGG 1 cut(s) 434
VpaK11BI GGWCC 2 cut(s) 147, 329
XapI RAATTY 1 cut(s) 311
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.