pycom16g12380

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013145
Physical Location & Seq
Reverse (-)
8690104 .. 8691291
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
ATGTGGTGGTCTCAGTCAAAACCACTCATCACCACCGTCAACGTCCTCCTCCTATCACTCCACCTCCACCTCCTCCCCACCCCCACTCTCTCCCAATGCCGCACCTCCTGCGGCCCCATCCCCATACACTACCCTTTCGCCCTCGATGACGGCTGTGGCGCGCCCCAGTTCCGCCACATGTTCAACTGCTCCACCGACCTCTTCTTCGCCACCCCATCCGGAAACTACAAAGTCCAGTCCATAAACTACGACAAGAAAACCATGGTCGTCTACGACCCCGCCATGTCCACCTGCTCCATCCTCCAGCCCCACCACGACTTCCTCATGACCCCAATCCAGTCCGCCATTATCCCGCCGACCTCCGACACCGTCTTCGCACTCCTCAACTGCTCCGTCGACTCCCCTGTCCTCAACCACTACAAGTACCTCTGCTTCAACTTCTCCGGCCACTCCTGTGACGAGCTCTACGACTCCTGCAACGCTTTTCGAATTTTCCGTACCGGTTCCGGATTGGCCACTCCTACCCCCAGCCCCGGAGGATTTTTAAGCAACGGTACTACCCCCAGTCCCGGAGGATTTTTAAGCAACGGTACTACCCCCAGTCCCGGAGGATTTCTAACCAACGGTACTCCTCCTCCTCCTGCTACTACGACTACTTCTCCGCCGTGTTGTTTTACGGGGTACGATTCGGTGAAGTTCATGAGCATGAATATATTGGACTGCACGCATTATACTAGCGTGACGAATACGGATGGATTGAAAGGGTTAGGACCGCCGGATTGGGTTTACGGGATTAAGCTGAGCTTCGCGGTGCCGGATACCGGGTGTGAGAGGTGTGCGCAGTCCGGTGGGACTTGTGGGTTTGATACCGAGACTGAAGGGTCTCTCTGCCTCTGCTCAACTTCCTCTAATTTCACTAGACAATGTGGTGCAGGCAGTTTTTCAGCGGTAGGACGGGACTGTTCTCCCAATATACTATTCCAAGCATCCTTTGTGATTTTGGGGGCGTTGTTAGTCTTTTTGCATGAGTTTAGGATGCTGAATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

350

Amino Acids

37.55

Weight (kDa)

5.87

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 92 3.9e-12 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 264 - 300 6.6e-06 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17350 AT3G17350
fragaria_vesca FvH4_4g21130
malus_domestica MD16G1139400.v1.1
prunus_persica Prupe.1G108900_v2.0.a1
pyrus_communis pycom16g12380
rosa_chinensis RchiOBHm_Chr4g0426711
rosa_laevigata RLG00000007284
rosa_multiflora Rmu_co8392541.1_g000001 Rmu_sc0010934.1_g000002 Rmu_sc0021908.1_g000002
rosa_roxburghii Rroxscaffold_5G00368680
rosa_rugosa Rorug04G0214400
rosa_samantha Rh4AG269400 Rh4BG275700 Rh4CG290200 Rh4DG272700
rosa_wichuraiana Rw4G023370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 299
AasI GACNNNNNNGTC 2 cut(s) 404, 880
Acc16I TGCGCA 1 cut(s) 840
Acc36I ACCTGC 1 cut(s) 299
AccB1I GGYRCC 1 cut(s) 811
AccI GTMKAC 2 cut(s) 270, 396
AccII CGCG 2 cut(s) 161, 809
AccIII TCCGGA 2 cut(s) 218, 506
AcoI YGGCCR 2 cut(s) 445, 513
AcsI RAATTY 2 cut(s) 489, 1042
AcuI CTGAAG 1 cut(s) 897
AfaI GTAC 6 cut(s) 425, 499, 556, 592, 628, 683
AfiI CCNNNNNNNGG 4 cut(s) 533, 569, 605, 821
AflIII ACRYGT 1 cut(s) 177
AgeI ACCGGT 1 cut(s) 500
AgsI TTSAA 3 cut(s) 184, 436, 760
AjuI GAANNNNNNNTTGG 2 cut(s) 962, 994
AleI CACNNNNGTG 1 cut(s) 453
AluBI AGCT 3 cut(s) 463, 799, 804
AluI AGCT 3 cut(s) 463, 799, 804
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 3 cut(s) 15, 866, 888
Aor13HI TCCGGA 2 cut(s) 218, 506
AoxI GGCC 3 cut(s) 112, 445, 513
ApoI RAATTY 2 cut(s) 489, 1042
ArsI GACNNNNNNTTYG 4 cut(s) 188, 220, 356, 388
AscI GGCGCGCC 1 cut(s) 159
AsiGI ACCGGT 1 cut(s) 500
AspLEI GCGC 3 cut(s) 161, 163, 841
AspS9I GGNCC 2 cut(s) 113, 770
AsuC2I CCSGG 4 cut(s) 534, 570, 606, 823
AsuHPI GGTGA 2 cut(s) 22, 703
AsuII TTCGAA 1 cut(s) 487
AvaII GGWCC 1 cut(s) 770
BalI TGGCCA 1 cut(s) 515
BanI GGYRCC 1 cut(s) 811
BanII GRGCYC 1 cut(s) 465
BarI GAAGNNNNNNTAC 2 cut(s) 416, 448
BbsI GAAGAC 1 cut(s) 364
Bbv12I GWGCWC 1 cut(s) 465
BccI CCATC 4 cut(s) 125, 223, 305, 746
BceAI ACGGC 2 cut(s) 166, 649
BciVI GTATCC 1 cut(s) 811
BcnI CCSGG 4 cut(s) 534, 570, 606, 823
BcoDI GTCTC 3 cut(s) 15, 866, 888
BfaI CTAG 2 cut(s) 735, 918
BfuAI ACCTGC 1 cut(s) 299
BfuI GTATCC 1 cut(s) 811
BisI GCNGC 2 cut(s) 100, 112
BlpI GCTNAGC 1 cut(s) 800
BlsI GCNGC 2 cut(s) 101, 113
Bme1390I CCNGG 4 cut(s) 534, 570, 606, 823
Bme18I GGWCC 1 cut(s) 770
BmgT120I GGNCC 2 cut(s) 113, 770
BmiI GGNNCC 3 cut(s) 115, 505, 813
BmrFI CCNGG 4 cut(s) 534, 570, 606, 823
BmrI ACTGGG 3 cut(s) 160, 558, 594
BmsI GCATC 2 cut(s) 995, 1026
BmuI ACTGGG 3 cut(s) 160, 558, 594
BpiI GAAGAC 1 cut(s) 364
BpmI CTGGAG 1 cut(s) 287
Bpu1102I GCTNAGC 1 cut(s) 800
Bpu14I TTCGAA 1 cut(s) 487
BpuMI CCSGG 4 cut(s) 534, 570, 606, 823
BsaI GGTCTC 2 cut(s) 15, 888
BsaJI CCNNGG 2 cut(s) 261, 532
BsaWI WCCGGW 4 cut(s) 218, 500, 506, 845
BsaXI ACNNNNNCTCC 6 cut(s) 48, 78, 619, 643, 649, 673
Bsc4I CCNNNNNNNGG 4 cut(s) 533, 569, 605, 821
Bse118I RCCGGY 1 cut(s) 500
Bse1I ACTGG 5 cut(s) 166, 235, 337, 564, 600
BseAI TCCGGA 2 cut(s) 218, 506
BseDI CCNNGG 2 cut(s) 261, 532
BseGI GGATG 6 cut(s) 117, 215, 297, 757, 986, 1041
BseLI CCNNNNNNNGG 4 cut(s) 533, 569, 605, 821
BseMII CTCAG 2 cut(s) 26, 791
BseNI ACTGG 5 cut(s) 166, 235, 337, 564, 600
BsePI GCGCGC 1 cut(s) 159
BseRI GAGGAG 6 cut(s) 38, 62, 371, 621, 624, 627
BseYI CCCAGC 1 cut(s) 527
BsgI GTGCAG 2 cut(s) 706, 951
Bsh1236I CGCG 2 cut(s) 161, 809
BshFI GGCC 3 cut(s) 114, 447, 515
BshNI GGYRCC 1 cut(s) 811
BshTI ACCGGT 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 465
BslFI GGGAC 4 cut(s) 552, 588, 865, 971
BslI CCNNNNNNNGG 4 cut(s) 533, 569, 605, 821
BsmAI GTCTC 3 cut(s) 15, 866, 888
BsmFI GGGAC 4 cut(s) 552, 588, 865, 971
BsnI GGCC 3 cut(s) 114, 447, 515
Bso31I GGTCTC 2 cut(s) 15, 888
Bsp119I TTCGAA 1 cut(s) 487
Bsp1286I GDGCHC 1 cut(s) 465
Bsp13I TCCGGA 2 cut(s) 218, 506
Bsp1720I GCTNAGC 1 cut(s) 800
Bsp19I CCATGG 1 cut(s) 261
BspANI GGCC 3 cut(s) 114, 447, 515
BspCNI CTCAG 2 cut(s) 25, 792
BspEI TCCGGA 2 cut(s) 218, 506
BspFNI CGCG 2 cut(s) 161, 809
BspHI TCATGA 2 cut(s) 324, 699
BspLI GGNNCC 3 cut(s) 115, 505, 813
BspMI ACCTGC 1 cut(s) 299
BspT104I TTCGAA 1 cut(s) 487
BspT107I GGYRCC 1 cut(s) 811
BspTNI GGTCTC 2 cut(s) 15, 888
BsrFI RCCGGY 1 cut(s) 500
BsrI ACTGG 5 cut(s) 166, 235, 337, 564, 600
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 2 cut(s) 261, 532
BssHII GCGCGC 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 261
Bst4CI ACNGT 6 cut(s) 37, 370, 554, 590, 626, 962
Bst6I CTCTTC 1 cut(s) 206
BstAPI GCANNNNNTGC 1 cut(s) 108
BstBI TTCGAA 1 cut(s) 487
BstC8I GCNNGC 3 cut(s) 161, 725, 934
BstDEI CTNAG 2 cut(s) 12, 800
BstDSI CCRYGG 1 cut(s) 261
BstF5I GGATG 6 cut(s) 117, 215, 297, 757, 986, 1041
BstFNI CGCG 2 cut(s) 161, 809
BstHHI GCGC 3 cut(s) 161, 163, 841
BstMAI GTCTC 3 cut(s) 15, 866, 888
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstNSI RCATGY 1 cut(s) 181
BstSCI CCNGG 4 cut(s) 532, 568, 604, 821
BstUI CGCG 2 cut(s) 161, 809
BstV2I GAAGAC 1 cut(s) 364
BsuI GTATCC 1 cut(s) 811
BsuRI GGCC 3 cut(s) 114, 447, 515
BtgI CCRYGG 1 cut(s) 261
BtsCI GGATG 6 cut(s) 117, 215, 297, 757, 986, 1041
BveI ACCTGC 1 cut(s) 299
Cac8I GCNNGC 3 cut(s) 161, 725, 934
CciI TCATGA 2 cut(s) 324, 699
CfoI GCGC 3 cut(s) 161, 163, 841
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 2 cut(s) 113, 770
Csp6I GTAC 6 cut(s) 424, 498, 555, 591, 627, 682
CspAI ACCGGT 1 cut(s) 500
CviAII CATG 7 cut(s) 178, 262, 283, 325, 700, 706, 1025
CviJI RGCY 9 cut(s) 114, 153, 307, 447, 463, 515, 531, 799, 804
CviKI_1 RGCY 9 cut(s) 114, 153, 307, 447, 463, 515, 531, 799, 804
CviQI GTAC 6 cut(s) 424, 498, 555, 591, 627, 682
DdeI CTNAG 2 cut(s) 12, 800
DrdI GACNNNNNNGTC 2 cut(s) 404, 880
DseDI GACNNNNNNGTC 2 cut(s) 404, 880
EaeI YGGCCR 2 cut(s) 445, 513
Eam1104I CTCTTC 1 cut(s) 206
EarI CTCTTC 1 cut(s) 206
EciI GGCGGA 3 cut(s) 161, 331, 651
Ecl136II GAGCTC 1 cut(s) 463
Eco130I CCWWGG 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 465
Eco31I GGTCTC 2 cut(s) 15, 888
Eco47I GGWCC 1 cut(s) 770
Eco53kI GAGCTC 1 cut(s) 463
Eco57I CTGAAG 1 cut(s) 897
EcoICRI GAGCTC 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 261
EcoT38I GRGCYC 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 7 cut(s) 181, 265, 286, 328, 703, 709, 1028
FalI AAGNNNNNCTT 2 cut(s) 788, 820
FaqI GGGAC 4 cut(s) 552, 588, 865, 971
FatI CATG 7 cut(s) 177, 261, 282, 324, 699, 705, 1024
FauI CCCGC 2 cut(s) 286, 360
FblI GTMKAC 2 cut(s) 270, 396
Fnu4HI GCNGC 2 cut(s) 100, 112
FokI GGATG 5 cut(s) 104, 202, 284, 764, 973
FriOI GRGCYC 1 cut(s) 465
Fsp4HI GCNGC 2 cut(s) 100, 112
FspBI CTAG 2 cut(s) 735, 918
FspI TGCGCA 1 cut(s) 840
GlaI GCGC 3 cut(s) 160, 162, 840
GluI GCNGC 2 cut(s) 100, 112
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 3 cut(s) 114, 447, 515
HhaI GCGC 3 cut(s) 161, 163, 841
Hin1II CATG 7 cut(s) 181, 265, 286, 328, 703, 709, 1028
Hin6I GCGC 3 cut(s) 159, 161, 839
HinP1I GCGC 3 cut(s) 159, 161, 839
HincII GTYRAC 2 cut(s) 40, 397
HindII GTYRAC 2 cut(s) 40, 397
HinfI GANTC 3 cut(s) 398, 470, 686
HphI GGTGA 2 cut(s) 22, 703
Hpy166II GTNNAC 5 cut(s) 40, 271, 288, 397, 787
Hpy188I TCNGA 1 cut(s) 364
Hpy188III TCNNGA 4 cut(s) 219, 325, 507, 700
Hpy8I GTNNAC 5 cut(s) 40, 271, 288, 397, 787
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 1 cut(s) 872
HpyCH4III ACNGT 6 cut(s) 37, 370, 554, 590, 626, 962
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 4 cut(s) 477, 723, 932, 1024
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpyF3I CTNAG 2 cut(s) 12, 800
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 7 cut(s) 181, 265, 286, 328, 703, 709, 1028
HspAI GCGC 3 cut(s) 159, 161, 839
Kpn2I TCCGGA 2 cut(s) 218, 506
LmnI GCTCC 3 cut(s) 194, 299, 395
LweI GCATC 2 cut(s) 995, 1026
MaeI CTAG 2 cut(s) 735, 918
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 2 cut(s) 455, 739
MboII GAAGA 3 cut(s) 193, 196, 364
MhlI GDGCHC 1 cut(s) 465
MlsI TGGCCA 1 cut(s) 515
MluCI AATT 3 cut(s) 489, 910, 1042
MluNI TGGCCA 1 cut(s) 515
MlyI GAGTC 2 cut(s) 392, 464
MmeI TCCRAC 1 cut(s) 387
Mox20I TGGCCA 1 cut(s) 515
MroI TCCGGA 2 cut(s) 218, 506
MscI TGGCCA 1 cut(s) 515
MseI TTAA 3 cut(s) 545, 581, 795
MslI CAYNNNNRTG 2 cut(s) 453, 704
Msp20I TGGCCA 1 cut(s) 515
MspA1I CMGCKG 1 cut(s) 947
MspR9I CCNGG 4 cut(s) 534, 570, 606, 823
MvnI CGCG 2 cut(s) 161, 809
MwoI GCNNNNNNNGC 1 cut(s) 108
NciI CCSGG 4 cut(s) 534, 570, 606, 823
NcoI CCATGG 1 cut(s) 261
NlaIII CATG 7 cut(s) 181, 265, 286, 328, 703, 709, 1028
NlaIV GGNNCC 3 cut(s) 115, 505, 813
NmuCI GTSAC 2 cut(s) 455, 739
NsbI TGCGCA 1 cut(s) 840
NspI RCATGY 1 cut(s) 181
NspV TTCGAA 1 cut(s) 487
OliI CACNNNNGTG 1 cut(s) 453
PagI TCATGA 2 cut(s) 324, 699
PalAI GGCGCGCC 1 cut(s) 159
PaqCI CACCTGC 1 cut(s) 299
PauI GCGCGC 1 cut(s) 159
PciI ACATGT 1 cut(s) 177
PcsI WCGNNNNNNNCGW 2 cut(s) 465, 493
PfeI GAWTC 1 cut(s) 686
PflFI GACNNNGTC 1 cut(s) 368
PfoI TCCNGGA 2 cut(s) 568, 604
PinAI ACCGGT 1 cut(s) 500
PkrI GCNGC 2 cut(s) 101, 113
PleI GAGTC 2 cut(s) 392, 464
PpsI GAGTC 2 cut(s) 392, 464
PscI ACATGT 1 cut(s) 177
Psp124BI GAGCTC 1 cut(s) 465
PspFI CCCAGC 1 cut(s) 527
PspN4I GGNNCC 3 cut(s) 115, 505, 813
PspPI GGNCC 2 cut(s) 113, 770
PsyI GACNNNGTC 1 cut(s) 368
PteI GCGCGC 1 cut(s) 159
RsaI GTAC 6 cut(s) 425, 499, 556, 592, 628, 683
RsaNI GTAC 6 cut(s) 424, 498, 555, 591, 627, 682
RseI CAYNNNNRTG 2 cut(s) 453, 704
SacI GAGCTC 1 cut(s) 465
SalI GTCGAC 1 cut(s) 395
SaqAI TTAA 3 cut(s) 545, 581, 795
SatI GCNGC 2 cut(s) 100, 112
Sau96I GGNCC 2 cut(s) 113, 770
SchI GAGTC 2 cut(s) 392, 464
ScrFI CCNGG 4 cut(s) 534, 570, 606, 823
SduI GDGCHC 1 cut(s) 465
SfaNI GCATC 2 cut(s) 995, 1026
SfuI TTCGAA 1 cut(s) 487
SgsI GGCGCGCC 1 cut(s) 159
SinI GGWCC 1 cut(s) 770
SmiMI CAYNNNNRTG 2 cut(s) 453, 704
Sse9I AATT 3 cut(s) 489, 910, 1042
SspMI CTAG 2 cut(s) 735, 918
SstI GAGCTC 1 cut(s) 465
StyD4I CCNGG 4 cut(s) 532, 568, 604, 821
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 6 cut(s) 37, 370, 554, 590, 626, 962
TaiI ACGT 1 cut(s) 45
TaqI TCGA 3 cut(s) 144, 396, 487
TasI AATT 3 cut(s) 489, 910, 1042
TauI GCSGC 2 cut(s) 102, 114
TfiI GAWTC 1 cut(s) 686
Tru1I TTAA 3 cut(s) 545, 581, 795
Tru9I TTAA 3 cut(s) 545, 581, 795
TseFI GTSAC 2 cut(s) 455, 739
Tsp45I GTSAC 2 cut(s) 455, 739
TspDTI ATGAA 2 cut(s) 688, 722
TspGWI ACGGA 3 cut(s) 382, 485, 764
Tth111I GACNNNGTC 1 cut(s) 368
VpaK11BI GGWCC 1 cut(s) 770
XapI RAATTY 2 cut(s) 489, 1042
XceI RCATGY 1 cut(s) 181
XmiI GTMKAC 2 cut(s) 270, 396
XspI CTAG 2 cut(s) 735, 918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.