pycom16g12930

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0015096
Physical Location & Seq
Reverse (-)
9154100 .. 9156860
2761 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 573 bp
ATGGCTACGGCATCGCCATCATCGTCGTCGTCGGCGGTGACGGAGCGGAGAGGAATACCAGGAGCTCAGTTCGTAGAGGACGTGCAGACCTATCTCACTCAGTTAGGCCTCGACGTTAACTCTGCCCTTGCCTTTCTCCAAGAAAGACTTCAGCAATACAAATTGGTTGAAATGAAGCTTCAAGCTCAGCAGAGAGATCTTCAGGCAAAGATTCCTGATATTGAGAAGTGCTTAGATGTTGTTGCTACTTTGCAAGCTAAGAAGGGTACTGGTGAGGAACTTATTACAGATTTTGAGGTATCTGAAGGGATATATTCACGAGCTAGCATTGACGATACCGATTCAGTCTGTTTATGGCTGGGTGCGAACGTCATGTTGGAGTATTCTTGTGAAGAGGCTACTGCTCTTCTACGAAAGAACTTAGACAATGCTAGAGCCAGTTTAGAAGTTCTGCTTGCTGATCTACAATTCTTGAGAGATCAAGTGACAATAACCCAGGTAACAATTGCTCGGGTGTACAACTGGGATGTTTATCAGCGCAGGATTCGACAAGCTTCCCCCAAGGATTCATGA

Protein Analysis

191

Amino Acids

21.24

Weight (kDa)

4.73

Isoelectric Point (pI)

47.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin PF02996 53 - 175 9.3e-31 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015096)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49510 AT5G49510
fragaria_vesca FvH4_4g20250
malus_domestica MD13G1150800.v1.1 MD16G1151300.v1.1
prunus_persica Prupe.1G115000_v2.0.a1
pyrus_communis pycom13g12960 pycom16g12930
rosa_chinensis RchiOBHm_Chr4g0425791
rosa_multiflora Rmu_sc0002094.1_g000001 Rmu_sc0010899.1_g000002
rosa_roxburghii Rroxscaffold_5G00367780
rosa_rugosa Rorug04G0206200
rosa_samantha Rh4AG263400 Rh4BG269300
rosa_wichuraiana Rw4G022730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 46
AciI CCGC 2 cut(s) 35, 46
AcuI CTGAAG 3 cut(s) 134, 185, 324
AfaI GTAC 2 cut(s) 268, 518
AgsI TTSAA 2 cut(s) 170, 182
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 2 cut(s) 58, 495
AjuI GAANNNNNNNTTGG 4 cut(s) 132, 164, 359, 391
AluBI AGCT 6 cut(s) 65, 178, 185, 257, 323, 554
AluI AGCT 6 cut(s) 65, 178, 185, 257, 323, 554
Alw21I GWGCWC 1 cut(s) 67
Ama87I CYCGRG 1 cut(s) 510
AoxI GGCC 1 cut(s) 106
Asp700I GAANNNNTTC 1 cut(s) 147
AspLEI GCGC 1 cut(s) 540
AsuHPI GGTGA 2 cut(s) 49, 284
AsuNHI GCTAGC 1 cut(s) 323
AvaI CYCGRG 1 cut(s) 510
BanII GRGCYC 1 cut(s) 67
BauI CACGAG 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 67
BccI CCATC 1 cut(s) 25
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 2 cut(s) 60, 497
BfaI CTAG 2 cut(s) 324, 432
BglII AGATCT 1 cut(s) 196
BlpI GCTNAGC 1 cut(s) 186
Bme1390I CCNGG 2 cut(s) 60, 497
BmeT110I CYCGRG 1 cut(s) 510
BmgBI CACGTC 1 cut(s) 82
BmrFI CCNGG 2 cut(s) 60, 497
BmrI ACTGGG 1 cut(s) 532
BmsI GCATC 1 cut(s) 20
BmtI GCTAGC 1 cut(s) 327
BmuI ACTGGG 1 cut(s) 532
Bpu1102I GCTNAGC 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 493
BsaBI GATNNNNATC 1 cut(s) 531
BsaJI CCNNGG 2 cut(s) 495, 561
Bse1I ACTGG 3 cut(s) 274, 438, 527
Bse8I GATNNNNATC 1 cut(s) 531
BseBI CCWGG 2 cut(s) 60, 497
BseDI CCNNGG 2 cut(s) 495, 561
BseGI GGATG 1 cut(s) 532
BseJI GATNNNNATC 1 cut(s) 531
BseMII CTCAG 3 cut(s) 80, 113, 200
BseNI ACTGG 3 cut(s) 274, 438, 527
BseYI CCCAGC 1 cut(s) 358
BsgI GTGCAG 1 cut(s) 104
BshFI GGCC 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 67
BsiHKCI CYCGRG 1 cut(s) 510
BsnI GGCC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 510
Bsp1286I GDGCHC 1 cut(s) 67
Bsp1407I TGTACA 1 cut(s) 516
Bsp143I GATC 3 cut(s) 196, 460, 478
Bsp1720I GCTNAGC 1 cut(s) 186
BspACI CCGC 2 cut(s) 35, 46
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 3 cut(s) 79, 112, 199
BspHI TCATGA 1 cut(s) 569
BspOI GCTAGC 1 cut(s) 327
BspQI GCTCTTC 1 cut(s) 411
BsrBI CCGCTC 1 cut(s) 46
BsrGI TGTACA 1 cut(s) 516
BsrI ACTGG 3 cut(s) 274, 438, 527
BssECI CCNNGG 2 cut(s) 495, 561
BssMI GATC 3 cut(s) 196, 460, 478
BssSI CACGAG 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 318
Bst2UI CCWGG 2 cut(s) 60, 497
Bst6I CTCTTC 2 cut(s) 387, 411
BstAUI TGTACA 1 cut(s) 516
BstC8I GCNNGC 3 cut(s) 255, 325, 456
BstDEI CTNAG 6 cut(s) 66, 99, 186, 232, 258, 421
BstF5I GGATG 1 cut(s) 532
BstHHI GCGC 1 cut(s) 540
BstKTI GATC 3 cut(s) 199, 463, 481
BstMBI GATC 3 cut(s) 196, 460, 478
BstNI CCWGG 2 cut(s) 60, 497
BstSCI CCNGG 2 cut(s) 58, 495
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BsuRI GGCC 1 cut(s) 108
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 1 cut(s) 532
Cac8I GCNNGC 3 cut(s) 255, 325, 456
CciI TCATGA 1 cut(s) 569
CfoI GCGC 1 cut(s) 540
Csp6I GTAC 2 cut(s) 267, 517
CviAII CATG 2 cut(s) 373, 570
CviQI GTAC 2 cut(s) 267, 517
DdeI CTNAG 6 cut(s) 66, 99, 186, 232, 258, 421
DpnI GATC 3 cut(s) 198, 462, 480
DpnII GATC 3 cut(s) 196, 460, 478
Eam1104I CTCTTC 2 cut(s) 387, 411
EarI CTCTTC 2 cut(s) 387, 411
Ecl136II GAGCTC 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 561
Eco147I AGGCCT 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 65
Eco57I CTGAAG 3 cut(s) 134, 185, 324
Eco88I CYCGRG 1 cut(s) 510
EcoICRI GAGCTC 1 cut(s) 65
EcoRII CCWGG 2 cut(s) 58, 495
EcoT14I CCWWGG 1 cut(s) 561
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 2 cut(s) 376, 573
FaiI YATR 4 cut(s) 313, 355, 374, 571
FalI AAGNNNNNCTT 4 cut(s) 132, 164, 438, 470
FatI CATG 2 cut(s) 372, 569
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 1 cut(s) 67
FspBI CTAG 2 cut(s) 324, 432
GlaI GCGC 1 cut(s) 539
GsaI CCCAGC 1 cut(s) 362
HaeIII GGCC 1 cut(s) 108
HhaI GCGC 1 cut(s) 540
Hin1II CATG 2 cut(s) 376, 573
Hin6I GCGC 1 cut(s) 538
HinP1I GCGC 1 cut(s) 538
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HindIII AAGCTT 2 cut(s) 176, 552
HinfI GANTC 4 cut(s) 211, 341, 544, 566
HpaI GTTAAC 1 cut(s) 118
HphI GGTGA 2 cut(s) 49, 284
Hpy166II GTNNAC 2 cut(s) 118, 517
Hpy188I TCNGA 1 cut(s) 304
Hpy188III TCNNGA 4 cut(s) 215, 318, 472, 570
Hpy8I GTNNAC 2 cut(s) 118, 517
Hpy99I CGWCG 4 cut(s) 28, 31, 34, 116
HpyAV CCTTC 2 cut(s) 256, 299
HpyCH4IV ACGT 3 cut(s) 81, 114, 369
HpyCH4V TGCA 2 cut(s) 85, 253
HpyF3I CTNAG 6 cut(s) 66, 99, 186, 232, 258, 421
HpySE526I ACGT 3 cut(s) 81, 114, 369
Hsp92II CATG 2 cut(s) 376, 573
HspAI GCGC 1 cut(s) 538
KspAI GTTAAC 1 cut(s) 118
Kzo9I GATC 3 cut(s) 196, 460, 478
LguI GCTCTTC 1 cut(s) 411
LmnI GCTCC 2 cut(s) 43, 62
LweI GCATC 1 cut(s) 20
MaeI CTAG 2 cut(s) 324, 432
MaeII ACGT 3 cut(s) 81, 114, 369
MaeIII GTNAC 3 cut(s) 37, 484, 499
MalI GATC 3 cut(s) 198, 462, 480
MbiI CCGCTC 1 cut(s) 46
MboI GATC 3 cut(s) 196, 460, 478
MboII GAAGA 3 cut(s) 191, 398, 404
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 3 cut(s) 161, 467, 504
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 6 cut(s) 44, 70, 119, 268, 289, 388
MroXI GAANNNNTTC 1 cut(s) 147
MseI TTAA 1 cut(s) 117
MspR9I CCNGG 2 cut(s) 60, 497
MunI CAATTG 1 cut(s) 504
MvaI CCWGG 2 cut(s) 60, 497
NdeII GATC 3 cut(s) 196, 460, 478
NheI GCTAGC 1 cut(s) 323
NlaIII CATG 2 cut(s) 376, 573
NmuCI GTSAC 2 cut(s) 37, 484
PagI TCATGA 1 cut(s) 569
PceI AGGCCT 1 cut(s) 108
PciSI GCTCTTC 1 cut(s) 411
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 78
PdmI GAANNNNTTC 1 cut(s) 147
PfeI GAWTC 4 cut(s) 211, 341, 544, 566
Psp124BI GAGCTC 1 cut(s) 67
Psp6I CCWGG 2 cut(s) 58, 495
PspFI CCCAGC 1 cut(s) 358
PspGI CCWGG 2 cut(s) 58, 495
PsuI RGATCY 1 cut(s) 196
RsaI GTAC 2 cut(s) 268, 518
RsaNI GTAC 2 cut(s) 267, 517
SacI GAGCTC 1 cut(s) 67
SapI GCTCTTC 1 cut(s) 411
SaqAI TTAA 1 cut(s) 117
Sau3AI GATC 3 cut(s) 196, 460, 478
ScrFI CCNGG 2 cut(s) 60, 497
SduI GDGCHC 1 cut(s) 67
SfaNI GCATC 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 472
SmoI CTYRAG 1 cut(s) 472
Sse9I AATT 3 cut(s) 161, 467, 504
SseBI AGGCCT 1 cut(s) 108
SsiI CCGC 2 cut(s) 35, 46
SspMI CTAG 2 cut(s) 324, 432
SstI GAGCTC 1 cut(s) 67
StuI AGGCCT 1 cut(s) 108
StyD4I CCNGG 2 cut(s) 58, 495
StyI CCWWGG 1 cut(s) 561
TaiI ACGT 3 cut(s) 84, 117, 372
TaqI TCGA 2 cut(s) 111, 547
TasI AATT 3 cut(s) 161, 467, 504
TatI WGTACW 1 cut(s) 516
TfiI GAWTC 4 cut(s) 211, 341, 544, 566
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TseFI GTSAC 2 cut(s) 37, 484
Tsp45I GTSAC 2 cut(s) 37, 484
TspDTI ATGAA 2 cut(s) 188, 558
TspGWI ACGGA 1 cut(s) 56
XmnI GAANNNNTTC 1 cut(s) 147
XspI CTAG 2 cut(s) 324, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.