pycom16g13170

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009608
Physical Location & Seq
Reverse (-)
9366984 .. 9368817
1834 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 924 bp
ATGGCGGAGGACAAGTACAACCTGAAGAACCCGGCGGTGAAGCGGATACTGCAGGAGGTTAAGGAGATGCAATCCAAACCCTCCGACGATTTCATGAGCCTCCCCCTCGAGGAGAACATATTTGACTGGCAATTTGCAATCAGAGGCCCTAGTGATACCGAATTCGAAGGTGGGATTTACCACGGGCGTATCCAGTTGCCGGCGGAGTACCCGTTCAAACCCCCTTCATTCATGTTGCTGACGCCAAATGGTCGCTTCGAAACCCAAACCAAGATTTGCTTGAGCATATCGAATCATCATCCCGAGCATTGGCAGCCATCATGGAGCGTGCGGACTGCTTTAGTTGCAATTATTGCATTCATGCCTACCAACCCAAATGGTGCATTAGGTTCACTGGAATACAAGAAGGAAGAAAGGCGTTCTCTGGCCATCAAATCTCGTGCAGCAGCCCCTAGATATGGCACTGCAGAACGCCAAAAGTTAATGGATGAGATTCATGAATGTATGCTGAGCAAGGCACCCCCTGTCCCTCAACTGAGCCCCTCACAGGCTTCGGAAGAGCATCCTTCAAACAAGGAAGGCGATGTTCAGGAGAGTTCCGAAAGCGCTGGAGCCACACCCCCTGAGGAAACTTCTGGGGAAGGGCCTGCAAACCCAACAGCGGGAGACAGGATTGTTGAAGAAGTACAGGAAGCCCCTCTGAATGCTAACCCTGCCCCTGAAGTAGCAAGGGCGTTGAATGGAGCTCCTTCCGCCGGATCAAGTCAGCTGCTACAGAGGCCAGAAGTTAAAGTACAAAAAGCAGCTGACGATCGCTTGTTCACATGGGCTGCTGTTGGACTTACCTTTGCAATCATGGTTCTGCTTTTTAAGAAGTTTATGAAATCCAGCGGACACGGTGCTGTTTTCATGGATGAATCTTAG

Protein Analysis

308

Amino Acids

33.81

Weight (kDa)

5.4

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 15 - 126 5.5e-20 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 107
AccB1I GGYRCC 1 cut(s) 517
AciI CCGC 8 cut(s) 5, 35, 43, 203, 331, 662, 753, 891
AclWI GGATC 1 cut(s) 766
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 1 cut(s) 161
AcuI CTGAAG 2 cut(s) 44, 741
AcyI GRCGYC 1 cut(s) 242
AfaI GTAC 4 cut(s) 17, 209, 687, 795
AfeI AGCGCT 1 cut(s) 607
AfiI CCNNNNNNNGG 8 cut(s) 109, 199, 309, 458, 547, 661, 662, 755
AgsI TTSAA 4 cut(s) 217, 570, 680, 739
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
AluBI AGCT 3 cut(s) 746, 769, 806
AluI AGCT 3 cut(s) 746, 769, 806
Alw21I GWGCWC 1 cut(s) 748
Alw26I GTCTC 1 cut(s) 660
AlwI GGATC 1 cut(s) 766
Ama87I CYCGRG 2 cut(s) 107, 302
Aor51HI AGCGCT 1 cut(s) 607
AoxI GGCC 4 cut(s) 145, 426, 644, 779
ApeKI GCWGC 6 cut(s) 313, 443, 446, 769, 803, 830
ApoI RAATTY 1 cut(s) 161
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
AspLEI GCGC 1 cut(s) 608
AspS9I GGNCC 2 cut(s) 146, 644
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 1 cut(s) 49
AsuII TTCGAA 2 cut(s) 165, 258
AvaI CYCGRG 2 cut(s) 107, 302
AxyI CCTNAGG 1 cut(s) 624
BaeI ACNNNNGTAYC 2 cut(s) 172, 205
BalI TGGCCA 1 cut(s) 428
BanI GGYRCC 1 cut(s) 517
BanII GRGCYC 2 cut(s) 542, 748
BarI GAAGNNNNNNTAC 2 cut(s) 777, 809
BauI CACGAG 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 748
BbvI GCAGC 6 cut(s) 325, 455, 458, 756, 815, 817
BccI CCATC 2 cut(s) 325, 437
BciVI GTATCC 2 cut(s) 39, 200
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 660
BfaI CTAG 2 cut(s) 150, 453
BfmI CTRYAG 3 cut(s) 50, 465, 773
BfoI RGCGCY 1 cut(s) 609
BfuI GTATCC 2 cut(s) 39, 200
BisI GCNGC 6 cut(s) 314, 444, 447, 770, 804, 831
BlpI GCTNAGC 1 cut(s) 509
BlsI GCNGC 6 cut(s) 315, 445, 448, 771, 805, 832
Bme1390I CCNGG 1 cut(s) 32
BmeT110I CYCGRG 2 cut(s) 107, 302
BmgT120I GGNCC 2 cut(s) 146, 644
BmiI GGNNCC 2 cut(s) 519, 613
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 2 cut(s) 57, 571
BpmI CTGGAG 1 cut(s) 630
Bpu1102I GCTNAGC 1 cut(s) 509
Bpu14I TTCGAA 2 cut(s) 165, 258
BpuEI CTTGAG 1 cut(s) 301
BpuMI CCSGG 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 181
BsaXI ACNNNNNCTCC 3 cut(s) 29, 197, 227
Bsc4I CCNNNNNNNGG 8 cut(s) 109, 199, 309, 458, 547, 661, 662, 755
Bse118I RCCGGY 1 cut(s) 199
Bse1I ACTGG 3 cut(s) 131, 193, 399
Bse21I CCTNAGG 1 cut(s) 624
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 4 cut(s) 298, 493, 562, 919
BseLI CCNNNNNNNGG 8 cut(s) 109, 199, 309, 458, 547, 661, 662, 755
BseMII CTCAG 3 cut(s) 500, 527, 615
BseNI ACTGG 3 cut(s) 131, 193, 399
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 6 cut(s) 325, 455, 458, 756, 815, 817
BsgI GTGCAG 1 cut(s) 462
Bsh1285I CGRYCG 1 cut(s) 814
BshFI GGCC 4 cut(s) 147, 428, 646, 781
BshNI GGYRCC 1 cut(s) 517
BsiEI CGRYCG 1 cut(s) 814
BsiHKAI GWGCWC 1 cut(s) 748
BsiHKCI CYCGRG 2 cut(s) 107, 302
BsiSI CCGG 3 cut(s) 32, 200, 756
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 8 cut(s) 109, 199, 309, 458, 547, 661, 662, 755
BsmAI GTCTC 1 cut(s) 660
BsmFI GGGAC 1 cut(s) 512
BsmI GAATGC 2 cut(s) 356, 709
BsnI GGCC 4 cut(s) 147, 428, 646, 781
BsoBI CYCGRG 2 cut(s) 107, 302
Bsp119I TTCGAA 2 cut(s) 165, 258
Bsp1286I GDGCHC 2 cut(s) 542, 748
Bsp143I GATC 2 cut(s) 758, 811
Bsp1720I GCTNAGC 1 cut(s) 509
BspACI CCGC 8 cut(s) 5, 35, 43, 203, 331, 662, 753, 891
BspANI GGCC 4 cut(s) 147, 428, 646, 781
BspCNI CTCAG 3 cut(s) 501, 528, 616
BspHI TCATGA 2 cut(s) 93, 496
BspLI GGNNCC 2 cut(s) 519, 613
BspMAI CTGCAG 2 cut(s) 54, 469
BspPI GGATC 1 cut(s) 766
BspQI GCTCTTC 1 cut(s) 552
BspT104I TTCGAA 2 cut(s) 165, 258
BspT107I GGYRCC 1 cut(s) 517
BsrFI RCCGGY 1 cut(s) 199
BsrI ACTGG 3 cut(s) 131, 193, 399
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 2 cut(s) 758, 811
BssNI GRCGYC 1 cut(s) 242
BssSI CACGAG 1 cut(s) 438
Bst2BI CACGAG 1 cut(s) 438
Bst4CI ACNGT 1 cut(s) 899
Bst6I CTCTTC 1 cut(s) 552
BstACI GRCGYC 1 cut(s) 242
BstAPI GCANNNNNTGC 1 cut(s) 353
BstBI TTCGAA 2 cut(s) 165, 258
BstC8I GCNNGC 3 cut(s) 201, 329, 648
BstDEI CTNAG 4 cut(s) 509, 536, 624, 921
BstDSI CCRYGG 1 cut(s) 181
BstF5I GGATG 4 cut(s) 298, 493, 562, 919
BstH2I RGCGCY 1 cut(s) 609
BstHHI GCGC 1 cut(s) 608
BstKTI GATC 2 cut(s) 761, 814
BstMAI GTCTC 1 cut(s) 660
BstMBI GATC 2 cut(s) 758, 811
BstMCI CGRYCG 1 cut(s) 814
BstMWI GCNNNNNNNGC 7 cut(s) 49, 313, 344, 353, 713, 752, 778
BstSCI CCNGG 1 cut(s) 30
BstSFI CTRYAG 3 cut(s) 50, 465, 773
BstV1I GCAGC 6 cut(s) 325, 455, 458, 756, 815, 817
Bsu36I CCTNAGG 1 cut(s) 624
BsuI GTATCC 2 cut(s) 39, 200
BsuRI GGCC 4 cut(s) 147, 428, 646, 781
BtgI CCRYGG 1 cut(s) 181
BtgZI GCGATG 1 cut(s) 597
BtsCI GGATG 4 cut(s) 298, 493, 562, 919
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 2 cut(s) 392, 462
Cac8I GCNNGC 3 cut(s) 201, 329, 648
CciI TCATGA 2 cut(s) 93, 496
CfoI GCGC 1 cut(s) 608
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 2 cut(s) 146, 644
CseI GACGC 1 cut(s) 250
Csp6I GTAC 4 cut(s) 16, 208, 686, 794
CviAII CATG 8 cut(s) 94, 232, 321, 361, 497, 825, 856, 910
CviQI GTAC 4 cut(s) 16, 208, 686, 794
DdeI CTNAG 4 cut(s) 509, 536, 624, 921
DpnI GATC 2 cut(s) 760, 813
DpnII GATC 2 cut(s) 758, 811
EaeI YGGCCR 1 cut(s) 426
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
EciI GGCGGA 3 cut(s) 20, 218, 742
Ecl136II GAGCTC 1 cut(s) 746
Eco24I GRGCYC 2 cut(s) 542, 748
Eco47III AGCGCT 1 cut(s) 607
Eco53kI GAGCTC 1 cut(s) 746
Eco57I CTGAAG 2 cut(s) 44, 741
Eco81I CCTNAGG 1 cut(s) 624
Eco88I CYCGRG 2 cut(s) 107, 302
EcoICRI GAGCTC 1 cut(s) 746
EcoO109I RGGNCCY 2 cut(s) 146, 644
EcoRI GAATTC 1 cut(s) 161
EcoT38I GRGCYC 2 cut(s) 542, 748
FaeI CATG 8 cut(s) 97, 235, 324, 364, 500, 828, 859, 913
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 1 cut(s) 512
FatI CATG 8 cut(s) 93, 231, 320, 360, 496, 824, 855, 909
FauI CCCGC 1 cut(s) 655
Fnu4HI GCNGC 6 cut(s) 314, 444, 447, 770, 804, 831
FokI GGATG 3 cut(s) 285, 500, 549
FriOI GRGCYC 2 cut(s) 542, 748
Fsp4HI GCNGC 6 cut(s) 314, 444, 447, 770, 804, 831
FspBI CTAG 2 cut(s) 150, 453
GlaI GCGC 1 cut(s) 607
GluI GCNGC 6 cut(s) 314, 444, 447, 770, 804, 831
GsuI CTGGAG 1 cut(s) 630
HaeII RGCGCY 1 cut(s) 609
HaeIII GGCC 4 cut(s) 147, 428, 646, 781
HapII CCGG 3 cut(s) 32, 200, 756
HgaI GACGC 1 cut(s) 250
HhaI GCGC 1 cut(s) 608
Hin1I GRCGYC 1 cut(s) 242
Hin1II CATG 8 cut(s) 97, 235, 324, 364, 500, 828, 859, 913
Hin6I GCGC 1 cut(s) 606
HinP1I GCGC 1 cut(s) 606
HinfI GANTC 3 cut(s) 292, 493, 917
HpaII CCGG 3 cut(s) 32, 200, 756
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 2 cut(s) 392, 822
Hpy188I TCNGA 5 cut(s) 85, 143, 556, 601, 702
Hpy188III TCNNGA 4 cut(s) 94, 302, 497, 590
Hpy8I GTNNAC 2 cut(s) 392, 822
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 7 cut(s) 161, 234, 400, 572, 576, 635, 759
HpyCH4III ACNGT 1 cut(s) 899
HpyF10VI GCNNNNNNNGC 7 cut(s) 49, 313, 344, 353, 713, 752, 778
HpyF3I CTNAG 4 cut(s) 509, 536, 624, 921
Hsp92I GRCGYC 1 cut(s) 242
Hsp92II CATG 8 cut(s) 97, 235, 324, 364, 500, 828, 859, 913
HspAI GCGC 1 cut(s) 606
KroI GCCGGC 1 cut(s) 199
KroNI GCCGGC 1 cut(s) 201
Kzo9I GATC 2 cut(s) 758, 811
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 4 cut(s) 324, 611, 743, 751
Lsp1109I GCAGC 6 cut(s) 325, 455, 458, 756, 815, 817
LweI GCATC 2 cut(s) 57, 571
MaeI CTAG 2 cut(s) 150, 453
MalI GATC 2 cut(s) 760, 813
MboI GATC 2 cut(s) 758, 811
MboII GAAGA 4 cut(s) 37, 422, 569, 692
MhlI GDGCHC 2 cut(s) 542, 748
MlsI TGGCCA 1 cut(s) 428
MluCI AATT 3 cut(s) 131, 161, 348
MluNI TGGCCA 1 cut(s) 428
MmeI TCCRAC 2 cut(s) 108, 817
Mox20I TGGCCA 1 cut(s) 428
MroNI GCCGGC 1 cut(s) 199
MscI TGGCCA 1 cut(s) 428
MseI TTAA 4 cut(s) 60, 482, 789, 870
Msp20I TGGCCA 1 cut(s) 428
MspA1I CMGCKG 4 cut(s) 662, 769, 806, 891
MspI CCGG 3 cut(s) 32, 200, 756
MspR9I CCNGG 1 cut(s) 32
Mva1269I GAATGC 2 cut(s) 356, 709
MwoI GCNNNNNNNGC 7 cut(s) 49, 313, 344, 353, 713, 752, 778
NaeI GCCGGC 1 cut(s) 201
NciI CCSGG 1 cut(s) 32
NdeII GATC 2 cut(s) 758, 811
NgoMIV GCCGGC 1 cut(s) 199
NlaIII CATG 8 cut(s) 97, 235, 324, 364, 500, 828, 859, 913
NlaIV GGNNCC 2 cut(s) 519, 613
NspV TTCGAA 2 cut(s) 165, 258
PaeR7I CTCGAG 1 cut(s) 107
PagI TCATGA 2 cut(s) 93, 496
PciSI GCTCTTC 1 cut(s) 552
PctI GAATGC 2 cut(s) 356, 709
PdiI GCCGGC 1 cut(s) 201
PfeI GAWTC 3 cut(s) 292, 493, 917
PkrI GCNGC 6 cut(s) 315, 445, 448, 771, 805, 832
Ple19I CGATCG 1 cut(s) 814
Psp124BI GAGCTC 1 cut(s) 748
PspN4I GGNNCC 2 cut(s) 519, 613
PspPI GGNCC 2 cut(s) 146, 644
PspXI VCTCGAGB 1 cut(s) 107
PstI CTGCAG 2 cut(s) 54, 469
PvuI CGATCG 1 cut(s) 814
PvuII CAGCTG 2 cut(s) 769, 806
RsaI GTAC 4 cut(s) 17, 209, 687, 795
RsaNI GTAC 4 cut(s) 16, 208, 686, 794
SacI GAGCTC 1 cut(s) 748
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 4 cut(s) 60, 482, 789, 870
SatI GCNGC 6 cut(s) 314, 444, 447, 770, 804, 831
Sau3AI GATC 2 cut(s) 758, 811
Sau96I GGNCC 2 cut(s) 146, 644
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 2 cut(s) 542, 748
SetI ASST 8 cut(s) 24, 60, 172, 391, 748, 771, 808, 848
SfaNI GCATC 2 cut(s) 57, 571
SfcI CTRYAG 3 cut(s) 50, 465, 773
Sfr274I CTCGAG 1 cut(s) 107
SfuI TTCGAA 2 cut(s) 165, 258
SlaI CTCGAG 1 cut(s) 107
SmlI CTYRAG 2 cut(s) 107, 280
SmoI CTYRAG 2 cut(s) 107, 280
Sse9I AATT 3 cut(s) 131, 161, 348
SsiI CCGC 8 cut(s) 5, 35, 43, 203, 331, 662, 753, 891
SspMI CTAG 2 cut(s) 150, 453
SstI GAGCTC 1 cut(s) 748
StyD4I CCNGG 1 cut(s) 30
TaaI ACNGT 1 cut(s) 899
TaqI TCGA 4 cut(s) 108, 165, 258, 290
TasI AATT 3 cut(s) 131, 161, 348
TatI WGTACW 3 cut(s) 15, 685, 793
TfiI GAWTC 3 cut(s) 292, 493, 917
Tru1I TTAA 4 cut(s) 60, 482, 789, 870
Tru9I TTAA 4 cut(s) 60, 482, 789, 870
TscAI CASTG 2 cut(s) 399, 469
TseI GCWGC 6 cut(s) 313, 443, 446, 769, 803, 830
TspDTI ATGAA 8 cut(s) 82, 216, 220, 349, 485, 513, 896, 898
TspRI CASTG 2 cut(s) 399, 469
XapI RAATTY 1 cut(s) 161
XhoI CTCGAG 1 cut(s) 107
XspI CTAG 2 cut(s) 150, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.