pycom16g13830

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008628
Physical Location & Seq
Reverse (-)
9880373 .. 9882242
1870 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1284 bp
ATGGCCATTGCTGGAGGAATATCTTGCCACGCCATTCTTGTCTTGTGCTTAGCTGCATTGGCCTCTCGAGCCACTGCCTCGGTTGCCATTCCTCCGGCTGTGTTCGATGTTTTCCGTGGTAGTAATTTGGGTGTTCATGACAAAGCTGATCCTGATGAGAAGATCTTTAACGTGGTAGATTTCGGCGCCAAGCCTGATGGGAACCACGATGCAAGCATGAATTTCATAAAAACATGGCTTGCAGCATGCCACAACACTGGGAAATCAAGGGTTCTGATCCCACCAGGGACATACAAGAGTGGGCCAGTGATATTTCAAGGGCCATGCACCAGTTCCAAACCCGTAGTTGTTCAAGTTTTAGGGACCATAAAAGGCCTGAATGATCTGTCCTTATATGCAGAGCCATACTGGTTCTTGTTTGAGAATGTAGAAGGCTTGGTCATTATCGGCAACGGCGTTTTCGACGGACAAGGTTCAAGTGCCTGGAAAGTCGCTGGTGACTGCGGCAGTTCCAGCGATGGTTGCAGTCCACTCCCATCTAACATCAAATTCAACGGCGTTAGAGACGGGGTCATTCGAGGCATTAGTTCCCTCAATAGCAAAGGAGTTCACCTTTTCATCACTAACAGCCAGAATGTTAGGGTTCGCAGAGTGAATATCTCCGCCCCTCGAACAAGCCCCAACACTGATGGCATCCACATCAGCAACTCCAACAATGTCAAGGTCGCCAGAACTCACATTGCCACCGGTGATGATTGCATTGGCATGATTAAGGGATCAACCAACATTGCCATCAACAATGTAATTTGTGGCCCCGGACACGGCATTAGTGTTGGTAGCCTTGGCAAGTATGAGCACGAAAATGACGTGACAGGAATCACCGTGAAGAAGACCACATTTTTGAACACAGACAATGGGATTAGAATCAAGTCATGGCCAGGATCGGGTCCTAGTCGGGCATCGGGAATGGTTTTCAAGGACATCATCATGCAGAATGTTATGAACCCTATCATAATTGACCAAGAGTACTGCACTGGGGGATGCAACAAGAACCAGCCATCGCGCGTGCAGATCAGCAATGTCCACTACATAAACATTAGAGGGACAACACCGTCAAATGTGGCAGTAGATTTCATTTGCAGCAGCCTGGTTCCTTGCCAAAACATTCAGCTTCATGACATCGATTTGAAGTATACTGGTCCAAATGCTGCAGCTCTCACCAACGTATGTAAAAATGTAAAAGCTGGTTTTGGTGGCATACAAAACCCACCAGCTTGCCACTGA

Protein Analysis

428

Amino Acids

45.14

Weight (kDa)

8.7

Isoelectric Point (pI)

30.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 83 - 411 2.4e-83 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1111
AccB1I GGYRCC 1 cut(s) 185
AccI GTMKAC 1 cut(s) 1193
AccII CGCG 2 cut(s) 1063, 1065
AciI CCGC 2 cut(s) 504, 663
AclWI GGATC 4 cut(s) 143, 271, 784, 949
AcoI YGGCCR 2 cut(s) 3, 935
AcsI RAATTY 2 cut(s) 220, 548
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 1 cut(s) 1028
AfiI CCNNNNNNNGG 2 cut(s) 821, 944
AgeI ACCGGT 1 cut(s) 746
AgsI TTSAA 7 cut(s) 317, 353, 477, 553, 904, 976, 1189
AjiI CACGTC 1 cut(s) 868
AjnI CCWGG 4 cut(s) 283, 482, 937, 1146
AluBI AGCT 6 cut(s) 53, 146, 1171, 1214, 1244, 1274
AluI AGCT 6 cut(s) 53, 146, 1171, 1214, 1244, 1274
Alw21I GWGCWC 1 cut(s) 858
Alw26I GTCTC 1 cut(s) 558
AlwI GGATC 4 cut(s) 143, 271, 784, 949
Ama87I CYCGRG 1 cut(s) 66
AoxI GGCC 7 cut(s) 3, 60, 302, 320, 373, 811, 935
ApeKI GCWGC 6 cut(s) 53, 242, 1140, 1143, 1208, 1211
ApoI RAATTY 2 cut(s) 220, 548
ArsI GACNNNNNNTTYG 2 cut(s) 883, 915
AsiGI ACCGGT 1 cut(s) 746
AspLEI GCGC 2 cut(s) 188, 1065
AspS9I GGNCC 6 cut(s) 302, 320, 363, 812, 947, 1199
AsuC2I CCSGG 1 cut(s) 816
AsuHPI GGTGA 5 cut(s) 509, 602, 761, 871, 1210
AvaI CYCGRG 1 cut(s) 66
AvaII GGWCC 3 cut(s) 363, 947, 1199
BalI TGGCCA 2 cut(s) 5, 937
BanI GGYRCC 1 cut(s) 185
BbsI GAAGAC 1 cut(s) 896
Bbv12I GWGCWC 1 cut(s) 858
BbvI GCAGC 6 cut(s) 40, 254, 1152, 1155, 1195, 1223
BccI CCATC 6 cut(s) 191, 512, 544, 683, 800, 1066
BceAI ACGGC 3 cut(s) 469, 571, 838
BciT130I CCWGG 4 cut(s) 285, 484, 939, 1148
BcnI CCSGG 1 cut(s) 816
BcoDI GTCTC 1 cut(s) 558
BfaI CTAG 1 cut(s) 951
BfmI CTRYAG 1 cut(s) 1209
BfoI RGCGCY 1 cut(s) 189
BglII AGATCT 1 cut(s) 162
BisI GCNGC 7 cut(s) 54, 243, 505, 1141, 1144, 1209, 1212
BlpI GCTNAGC 1 cut(s) 49
BlsI GCNGC 7 cut(s) 55, 244, 506, 1142, 1145, 1210, 1213
BmcAI AGTACT 1 cut(s) 1028
Bme1390I CCNGG 5 cut(s) 285, 484, 816, 939, 1148
Bme18I GGWCC 3 cut(s) 363, 947, 1199
BmeT110I CYCGRG 1 cut(s) 66
BmgBI CACGTC 1 cut(s) 868
BmgT120I GGNCC 6 cut(s) 302, 320, 363, 812, 947, 1199
BmiI GGNNCC 6 cut(s) 187, 203, 364, 814, 948, 1152
BmrFI CCNGG 5 cut(s) 285, 484, 816, 939, 1148
BmrI ACTGGG 2 cut(s) 267, 1044
BmsI GCATC 4 cut(s) 199, 702, 968, 1031
BmuI ACTGGG 2 cut(s) 267, 1044
BpiI GAAGAC 1 cut(s) 896
BpmI CTGGAG 1 cut(s) 33
Bpu1102I GCTNAGC 1 cut(s) 49
BpuMI CCSGG 1 cut(s) 816
Bsa29I ATCGAT 1 cut(s) 1182
BsaBI GATNNNNATC 1 cut(s) 923
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 5 cut(s) 78, 115, 284, 814, 841
BsaWI WCCGGW 1 cut(s) 746
Bsc4I CCNNNNNNNGG 2 cut(s) 821, 944
Bse118I RCCGGY 1 cut(s) 746
Bse1I ACTGG 6 cut(s) 262, 305, 330, 413, 1039, 1201
Bse3DI GCAATG 4 cut(s) 6, 738, 786, 1084
Bse8I GATNNNNATC 1 cut(s) 923
BseBI CCWGG 4 cut(s) 285, 484, 939, 1148
BseCI ATCGAT 1 cut(s) 1182
BseDI CCNNGG 5 cut(s) 78, 115, 284, 814, 841
BseGI GGATG 2 cut(s) 693, 1046
BseJI GATNNNNATC 1 cut(s) 923
BseLI CCNNNNNNNGG 2 cut(s) 821, 944
BseMI GCAATG 4 cut(s) 6, 738, 786, 1084
BseNI ACTGG 6 cut(s) 262, 305, 330, 413, 1039, 1201
BseXI GCAGC 6 cut(s) 40, 254, 1152, 1155, 1195, 1223
BsgI GTGCAG 2 cut(s) 1015, 1088
Bsh1236I CGCG 2 cut(s) 1063, 1065
BshFI GGCC 7 cut(s) 5, 62, 304, 322, 375, 813, 937
BshNI GGYRCC 1 cut(s) 185
BshTI ACCGGT 1 cut(s) 746
BshVI ATCGAT 1 cut(s) 1182
BsiHKAI GWGCWC 1 cut(s) 858
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 3 cut(s) 95, 747, 816
BslFI GGGAC 3 cut(s) 301, 376, 1117
BslI CCNNNNNNNGG 2 cut(s) 821, 944
BsmAI GTCTC 1 cut(s) 558
BsmBI CGTCTC 1 cut(s) 558
BsmFI GGGAC 3 cut(s) 301, 376, 1117
BsnI GGCC 7 cut(s) 5, 62, 304, 322, 375, 813, 937
BsoBI CYCGRG 1 cut(s) 66
Bsp1286I GDGCHC 1 cut(s) 858
Bsp143I GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
Bsp1720I GCTNAGC 1 cut(s) 49
BspACI CCGC 2 cut(s) 504, 663
BspANI GGCC 7 cut(s) 5, 62, 304, 322, 375, 813, 937
BspDI ATCGAT 1 cut(s) 1182
BspFNI CGCG 2 cut(s) 1063, 1065
BspHI TCATGA 2 cut(s) 136, 1174
BspLI GGNNCC 6 cut(s) 187, 203, 364, 814, 948, 1152
BspMAI CTGCAG 1 cut(s) 1213
BspPI GGATC 4 cut(s) 143, 271, 784, 949
BspT107I GGYRCC 1 cut(s) 185
BsrDI GCAATG 4 cut(s) 6, 738, 786, 1084
BsrFI RCCGGY 1 cut(s) 746
BsrI ACTGG 6 cut(s) 262, 305, 330, 413, 1039, 1201
BssAI RCCGGY 1 cut(s) 746
BssECI CCNNGG 5 cut(s) 78, 115, 284, 814, 841
BssMI GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
BssNAI GTATAC 1 cut(s) 1194
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 1 cut(s) 841
Bst1107I GTATAC 1 cut(s) 1194
Bst2UI CCWGG 4 cut(s) 285, 484, 939, 1148
Bst4CI ACNGT 2 cut(s) 883, 1113
BstACI GRCGYC 1 cut(s) 186
BstC8I GCNNGC 5 cut(s) 214, 240, 247, 1067, 1276
BstDEI CTNAG 1 cut(s) 49
BstDSI CCRYGG 1 cut(s) 115
BstF5I GGATG 2 cut(s) 693, 1046
BstFNI CGCG 2 cut(s) 1063, 1065
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 2 cut(s) 188, 1065
BstKTI GATC 7 cut(s) 151, 165, 279, 385, 779, 944, 1074
BstMAI GTCTC 1 cut(s) 558
BstMBI GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
BstMWI GCNNNNNNNGC 5 cut(s) 59, 68, 83, 513, 522
BstNI CCWGG 4 cut(s) 285, 484, 939, 1148
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 5 cut(s) 283, 482, 814, 937, 1146
BstSFI CTRYAG 1 cut(s) 1209
BstUI CGCG 2 cut(s) 1063, 1065
BstV1I GCAGC 6 cut(s) 40, 254, 1152, 1155, 1195, 1223
BstV2I GAAGAC 1 cut(s) 896
BstX2I RGATCY 1 cut(s) 162
BstXI CCANNNNNNTGG 1 cut(s) 257
BstYI RGATCY 1 cut(s) 162
BstZ17I GTATAC 1 cut(s) 1194
Bsu15I ATCGAT 1 cut(s) 1182
BsuRI GGCC 7 cut(s) 5, 62, 304, 322, 375, 813, 937
BsuTUI ATCGAT 1 cut(s) 1182
BtgI CCRYGG 1 cut(s) 115
BtgZI GCGATG 2 cut(s) 531, 1044
BtrI CACGTC 1 cut(s) 868
BtsCI GGATG 2 cut(s) 693, 1046
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 6 cut(s) 72, 255, 312, 684, 1032, 1279
Cac8I GCNNGC 5 cut(s) 214, 240, 247, 1067, 1276
CciI TCATGA 2 cut(s) 136, 1174
CfoI GCGC 2 cut(s) 188, 1065
Cfr10I RCCGGY 1 cut(s) 746
Cfr13I GGNCC 6 cut(s) 302, 320, 363, 812, 947, 1199
ClaI ATCGAT 1 cut(s) 1182
Csp6I GTAC 1 cut(s) 1027
CspAI ACCGGT 1 cut(s) 746
CviAII CATG 9 cut(s) 137, 217, 234, 246, 324, 766, 933, 988, 1175
CviQI GTAC 1 cut(s) 1027
DdeI CTNAG 1 cut(s) 49
DinI GGCGCC 1 cut(s) 187
DpnI GATC 7 cut(s) 150, 164, 278, 384, 778, 943, 1073
DpnII GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
DrdI GACNNNNNNGTC 1 cut(s) 1111
DseDI GACNNNNNNGTC 1 cut(s) 1111
EaeI YGGCCR 2 cut(s) 3, 935
EciI GGCGGA 1 cut(s) 652
Eco130I CCWWGG 1 cut(s) 841
Eco147I AGGCCT 1 cut(s) 375
Eco47I GGWCC 3 cut(s) 363, 947, 1199
Eco88I CYCGRG 1 cut(s) 66
EcoO109I RGGNCCY 1 cut(s) 947
EcoRII CCWGG 4 cut(s) 283, 482, 937, 1146
EcoT14I CCWWGG 1 cut(s) 841
EgeI GGCGCC 1 cut(s) 187
EheI GGCGCC 1 cut(s) 187
ErhI CCWWGG 1 cut(s) 841
Esp3I CGTCTC 1 cut(s) 558
FaeI CATG 9 cut(s) 140, 220, 237, 249, 327, 769, 936, 991, 1178
FaqI GGGAC 3 cut(s) 301, 376, 1117
FatI CATG 9 cut(s) 136, 216, 233, 245, 323, 765, 932, 987, 1174
FblI GTMKAC 1 cut(s) 1193
Fnu4HI GCNGC 7 cut(s) 54, 243, 505, 1141, 1144, 1209, 1212
FokI GGATG 2 cut(s) 680, 1053
Fsp4HI GCNGC 7 cut(s) 54, 243, 505, 1141, 1144, 1209, 1212
FspBI CTAG 1 cut(s) 951
GlaI GCGC 2 cut(s) 187, 1064
GluI GCNGC 7 cut(s) 54, 243, 505, 1141, 1144, 1209, 1212
GsuI CTGGAG 1 cut(s) 33
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 7 cut(s) 5, 62, 304, 322, 375, 813, 937
HapII CCGG 3 cut(s) 95, 747, 816
HhaI GCGC 2 cut(s) 188, 1065
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 9 cut(s) 140, 220, 237, 249, 327, 769, 936, 991, 1178
Hin6I GCGC 2 cut(s) 186, 1063
HinP1I GCGC 2 cut(s) 186, 1063
HinfI GANTC 2 cut(s) 876, 924
HpaII CCGG 3 cut(s) 95, 747, 816
HphI GGTGA 5 cut(s) 509, 602, 761, 871, 1210
Hpy166II GTNNAC 4 cut(s) 530, 610, 1084, 1194
Hpy188I TCNGA 1 cut(s) 276
Hpy188III TCNNGA 5 cut(s) 66, 137, 152, 963, 1175
Hpy8I GTNNAC 4 cut(s) 530, 610, 1084, 1194
Hpy99I CGWCG 1 cut(s) 467
HpyAV CCTTC 1 cut(s) 425
HpyCH4III ACNGT 2 cut(s) 883, 1113
HpyCH4IV ACGT 3 cut(s) 171, 867, 1224
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 68, 83, 513, 522
HpyF3I CTNAG 1 cut(s) 49
HpySE526I ACGT 3 cut(s) 171, 867, 1224
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 9 cut(s) 140, 220, 237, 249, 327, 769, 936, 991, 1178
HspAI GCGC 2 cut(s) 186, 1063
KasI GGCGCC 1 cut(s) 185
Kzo9I GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
Lsp1109I GCAGC 6 cut(s) 40, 254, 1152, 1155, 1195, 1223
LweI GCATC 4 cut(s) 199, 702, 968, 1031
MaeI CTAG 1 cut(s) 951
MaeII ACGT 3 cut(s) 171, 867, 1224
MaeIII GTNAC 2 cut(s) 497, 868
MalI GATC 7 cut(s) 150, 164, 278, 384, 778, 943, 1073
MboI GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
MboII GAAGA 3 cut(s) 172, 898, 901
MflI RGATCY 1 cut(s) 162
MhlI GDGCHC 1 cut(s) 858
MlsI TGGCCA 2 cut(s) 5, 937
MluCI AATT 5 cut(s) 124, 220, 548, 804, 1014
MluNI TGGCCA 2 cut(s) 5, 937
Mly113I GGCGCC 1 cut(s) 186
MmeI TCCRAC 1 cut(s) 735
MnlI CCTC 8 cut(s) 8, 73, 88, 102, 572, 602, 678, 1094
Mox20I TGGCCA 2 cut(s) 5, 937
MscI TGGCCA 2 cut(s) 5, 937
MseI TTAA 2 cut(s) 168, 771
MslI CAYNNNNRTG 3 cut(s) 764, 861, 986
Msp20I TGGCCA 2 cut(s) 5, 937
MspI CCGG 3 cut(s) 95, 747, 816
MspR9I CCNGG 5 cut(s) 285, 484, 816, 939, 1148
MvaI CCWGG 4 cut(s) 285, 484, 939, 1148
MvnI CGCG 2 cut(s) 1063, 1065
MwoI GCNNNNNNNGC 5 cut(s) 59, 68, 83, 513, 522
NarI GGCGCC 1 cut(s) 186
NciI CCSGG 1 cut(s) 816
NdeII GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
NlaIII CATG 9 cut(s) 140, 220, 237, 249, 327, 769, 936, 991, 1178
NlaIV GGNNCC 6 cut(s) 187, 203, 364, 814, 948, 1152
NmuCI GTSAC 2 cut(s) 497, 868
NspI RCATGY 1 cut(s) 249
PaeI GCATGC 1 cut(s) 249
PaeR7I CTCGAG 1 cut(s) 66
PagI TCATGA 2 cut(s) 136, 1174
PceI AGGCCT 1 cut(s) 375
PcsI WCGNNNNNNNCGW 3 cut(s) 453, 459, 864
PfeI GAWTC 2 cut(s) 876, 924
PflFI GACNNNGTC 1 cut(s) 569
PinAI ACCGGT 1 cut(s) 746
PkrI GCNGC 7 cut(s) 55, 244, 506, 1142, 1145, 1210, 1213
PluTI GGCGCC 1 cut(s) 189
PpuMI RGGWCCY 1 cut(s) 947
Psp5II RGGWCCY 1 cut(s) 947
Psp6I CCWGG 4 cut(s) 283, 482, 937, 1146
PspGI CCWGG 4 cut(s) 283, 482, 937, 1146
PspN4I GGNNCC 6 cut(s) 187, 203, 364, 814, 948, 1152
PspPI GGNCC 6 cut(s) 302, 320, 363, 812, 947, 1199
PspPPI RGGWCCY 1 cut(s) 947
PstI CTGCAG 1 cut(s) 1213
PsuI RGATCY 1 cut(s) 162
PsyI GACNNNGTC 1 cut(s) 569
RsaI GTAC 1 cut(s) 1028
RsaNI GTAC 1 cut(s) 1027
RseI CAYNNNNRTG 3 cut(s) 764, 861, 986
SaqAI TTAA 2 cut(s) 168, 771
SatI GCNGC 7 cut(s) 54, 243, 505, 1141, 1144, 1209, 1212
Sau3AI GATC 7 cut(s) 148, 162, 276, 382, 776, 941, 1071
Sau96I GGNCC 6 cut(s) 302, 320, 363, 812, 947, 1199
ScaI AGTACT 1 cut(s) 1028
ScrFI CCNGG 5 cut(s) 285, 484, 816, 939, 1148
SduI GDGCHC 1 cut(s) 858
SfaNI GCATC 4 cut(s) 199, 702, 968, 1031
SfcI CTRYAG 1 cut(s) 1209
SfoI GGCGCC 1 cut(s) 187
Sfr274I CTCGAG 1 cut(s) 66
SgrAI CRCCGGYG 1 cut(s) 746
SinI GGWCC 3 cut(s) 363, 947, 1199
SlaI CTCGAG 1 cut(s) 66
SmiMI CAYNNNNRTG 3 cut(s) 764, 861, 986
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
SphI GCATGC 1 cut(s) 249
Sse9I AATT 5 cut(s) 124, 220, 548, 804, 1014
SseBI AGGCCT 1 cut(s) 375
SsiI CCGC 2 cut(s) 504, 663
SspDI GGCGCC 1 cut(s) 185
SspMI CTAG 1 cut(s) 951
StuI AGGCCT 1 cut(s) 375
StyD4I CCNGG 5 cut(s) 283, 482, 814, 937, 1146
StyI CCWWGG 1 cut(s) 841
TaaI ACNGT 2 cut(s) 883, 1113
TaiI ACGT 3 cut(s) 174, 870, 1227
TaqI TCGA 6 cut(s) 67, 105, 462, 577, 670, 1182
TasI AATT 5 cut(s) 124, 220, 548, 804, 1014
TatI WGTACW 1 cut(s) 1026
TauI GCSGC 1 cut(s) 507
TfiI GAWTC 2 cut(s) 876, 924
Tru1I TTAA 2 cut(s) 168, 771
Tru9I TTAA 2 cut(s) 168, 771
TscAI CASTG 5 cut(s) 79, 262, 312, 691, 1039
TseFI GTSAC 2 cut(s) 497, 868
TseI GCWGC 6 cut(s) 53, 242, 1140, 1143, 1208, 1211
Tsp45I GTSAC 2 cut(s) 497, 868
TspDTI ATGAA 7 cut(s) 125, 214, 233, 607, 1016, 1123, 1163
TspGWI ACGGA 2 cut(s) 104, 480
TspRI CASTG 5 cut(s) 79, 262, 312, 691, 1039
Tth111I GACNNNGTC 1 cut(s) 569
VpaK11BI GGWCC 3 cut(s) 363, 947, 1199
XapI RAATTY 2 cut(s) 220, 548
XceI RCATGY 1 cut(s) 249
XhoI CTCGAG 1 cut(s) 66
XmiI GTMKAC 1 cut(s) 1193
XspI CTAG 1 cut(s) 951
ZrmI AGTACT 1 cut(s) 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.