pycom16g14180

CLASP N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014024
Physical Location & Seq
Forward (+)
10218015 .. 10219703
1689 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1137 bp
ATGGTAGAAAACATCACCTTCATGCCACGTGTCTTGATAGAGCAGACTCACCCTAGAGTGGACAACAATTTATTCCCTACTTTCGTTATCTCCATCACACCATCCCTCATTCATCATCGAAAACCCAAAGATTTTCAGTCTCACATCTCTTACTCTCTCTCTCTCCCCCCATCACTGCCGTGTCCGTCTCCGATTTCAATGGCTATGGCTCTCAGACCCATCGACAATGCTCTCTCAACCACACCGGAAAGACCCAAAAAGCAAACCAAAGTTGCTGTTCCAATCCAGAAGCAGAAACCCGAATTCGGCCTCATAAATGACGAAAACCAGGCTCCGCTTCCTGCAACCGCTGATGCCGCCATCGACTACATCTCTTCCGAGAATCTCAAACCCATCTCAGACCCGGATCTCAAGATTCAGAGCTTGAATGAGGGATTGGAATCAAAGGATTGGGTGAAGGTGTGTGAGTCTCTGAACAATGTCAGGCGTTTTGCGCTCTACCATTCTGCCCTTCTGGCTCCTACCCTAGAGAAAGTTATGGTGGTGCTGGTCAAGGCAATGAAGAATCCGAGAAGTGCCTTAATCAAGACCTCGATTATGGCATCTTCCGACATCTTCGCCGCCTTTGGTGATGGTTTACTTGAGTCCGCAGCCTCTAATGCATTTGATCAATTGCTGCTGCAATTGCTGTTGAAGTCATCTCAAGACAAACGTTTTGTGTGTGAAGAAGCAGACAGGGCACTGAGCTCAGTGGTGCAGTCCTTGACCCCTCTTCCTCTGCTTCAGAAGCTTCGAGCCTACGCTTCTCATGGCAACCCTAGAGTCAGAGCCAAAGCTGCCATTTCTGTCGCATCTTGCGTCGCCAAGATGGGGCTTGAAGGAATGAAGGATTATGGGGTGGTTTCTTTGGTGAAAATGGCTGCAGACTTGTTGAATGATAGGCTGCCTGAGGCGAGAGAAGCAGCACGGGGAATTGTGATGTCGGTATACAATGCCTATACAGAGAATGAAGAGGAGAAGCTGCGGGAGAATGAAGAGGAGAAGCCGGAGAAGCAGGAGATTTGGCTAAGCTTTTGCCAGTCTAATTTGTCAACAATCCATGCTCAGTCCATGGTCAAAATTACTTCTTCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLASP_N

Protein Analysis

379

Amino Acids

41.85

Weight (kDa)

7.05

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLASP_N PF12348 178 - 341 6.5e-06 CLASP N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15830
fragaria_vesca FvH4_4g18210
malus_domestica MD13G1168000.v1.1 MD16G1168400.v1.1
prunus_persica Prupe.1G135600_v2.0.a1
pyrus_communis pycom13g14380 pycom16g14180
rosa_chinensis RchiOBHm_Chr4g0422361
rosa_laevigata RLG00000007616
rosa_multiflora Rmu_co8367675.1_g000001 Rmu_sc0013245.1_g000010
rosa_roxburghii Rroxscaffold_5G00364480
rosa_rugosa Rorug04G0179400
rosa_samantha Rh4AG238800 Rh4BG242400 Rh4CG253900
rosa_wichuraiana Rw4G020650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 987
AciI CCGC 6 cut(s) 335, 348, 357, 621, 648, 1024
AclI AACGTT 1 cut(s) 712
AclWI GGATC 1 cut(s) 414
AcsI RAATTY 1 cut(s) 302
AcuI CTGAAG 1 cut(s) 767
AcvI CACGTG 1 cut(s) 29
AfiI CCNNNNNNNGG 3 cut(s) 58, 305, 870
AflIII ACRYGT 1 cut(s) 28
AgsI TTSAA 5 cut(s) 198, 427, 694, 878, 934
AjnI CCWGG 1 cut(s) 327
AjuI GAANNNNNNNTTGG 2 cut(s) 419, 451
AleI CACNNNNGTG 1 cut(s) 178
AluBI AGCT 6 cut(s) 423, 747, 790, 836, 1021, 1071
AluI AGCT 6 cut(s) 423, 747, 790, 836, 1021, 1071
Alw21I GWGCWC 1 cut(s) 749
Alw26I GTCTC 3 cut(s) 144, 192, 474
AlwI GGATC 1 cut(s) 414
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 8 cut(s) 650, 676, 679, 836, 920, 943, 962, 1021
ApoI RAATTY 1 cut(s) 302
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
AspLEI GCGC 1 cut(s) 496
AsuC2I CCSGG 1 cut(s) 404
AsuHPI GGTGA 5 cut(s) 7, 41, 466, 641, 922
AxyI CCTNAGG 1 cut(s) 948
BaeGI GKGCMC 1 cut(s) 742
BanII GRGCYC 1 cut(s) 749
BbrPI CACGTG 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 749
BbvI GCAGC 8 cut(s) 662, 663, 666, 823, 907, 930, 974, 1008
BccI CCATC 8 cut(s) 101, 109, 178, 227, 368, 401, 626, 862
BceAI ACGGC 1 cut(s) 163
BciT130I CCWGG 1 cut(s) 329
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 1 cut(s) 404
BcoDI GTCTC 3 cut(s) 144, 192, 474
BfaI CTAG 3 cut(s) 54, 527, 819
BfmI CTRYAG 1 cut(s) 921
BglI GCCNNNNNGGC 1 cut(s) 515
BlpI GCTNAGC 1 cut(s) 1067
Bme1390I CCNGG 2 cut(s) 329, 404
BmiI GGNNCC 2 cut(s) 333, 519
BmrFI CCNGG 2 cut(s) 329, 404
BmsI GCATC 3 cut(s) 343, 611, 860
Bpu1102I GCTNAGC 1 cut(s) 1067
BpuEI CTTGAG 3 cut(s) 395, 662, 687
BpuMI CCSGG 1 cut(s) 404
BsaAI YACGTR 1 cut(s) 29
BsaBI GATNNNNATC 1 cut(s) 439
BsaJI CCNNGG 1 cut(s) 1110
BsaWI WCCGGW 1 cut(s) 244
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 305, 870
Bse1I ACTGG 1 cut(s) 1078
Bse21I CCTNAGG 1 cut(s) 948
Bse3DI GCAATG 1 cut(s) 564
Bse8I GATNNNNATC 1 cut(s) 439
BseBI CCWGG 1 cut(s) 329
BseDI CCNNGG 1 cut(s) 1110
BseGI GGATG 1 cut(s) 101
BseJI GATNNNNATC 1 cut(s) 439
BseLI CCNNNNNNNGG 3 cut(s) 58, 305, 870
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 6 cut(s) 226, 411, 734, 762, 939, 1118
BseNI ACTGG 1 cut(s) 1078
BseRI GAGGAG 2 cut(s) 1028, 1052
BseSI GKGCMC 1 cut(s) 742
BseXI GCAGC 8 cut(s) 662, 663, 666, 823, 907, 930, 974, 1008
BsgI GTGCAG 1 cut(s) 776
BshFI GGCC 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 749
BsiSI CCGG 3 cut(s) 245, 404, 1046
BslI CCNNNNNNNGG 3 cut(s) 58, 305, 870
BsmAI GTCTC 3 cut(s) 144, 192, 474
BsmBI CGTCTC 1 cut(s) 192
BsnI GGCC 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 742, 749
Bsp143I GATC 2 cut(s) 406, 667
Bsp1720I GCTNAGC 1 cut(s) 1067
Bsp19I CCATGG 1 cut(s) 1110
BspACI CCGC 6 cut(s) 335, 348, 357, 621, 648, 1024
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 6 cut(s) 225, 410, 735, 761, 940, 1117
BspLI GGNNCC 2 cut(s) 333, 519
BspMAI CTGCAG 1 cut(s) 925
BspPI GGATC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 564
BsrI ACTGG 1 cut(s) 1078
BssECI CCNNGG 1 cut(s) 1110
BssMI GATC 2 cut(s) 406, 667
BssNAI GTATAC 1 cut(s) 988
BssT1I CCWWGG 1 cut(s) 1110
Bst1107I GTATAC 1 cut(s) 988
Bst2UI CCWGG 1 cut(s) 329
Bst6I CTCTTC 4 cut(s) 379, 777, 1005, 1029
BstBAI YACGTR 1 cut(s) 29
BstDEI CTNAG 8 cut(s) 212, 397, 743, 748, 948, 1067, 1104, 1131
BstDSI CCRYGG 1 cut(s) 1110
BstF5I GGATG 1 cut(s) 101
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 2 cut(s) 409, 670
BstMAI GTCTC 3 cut(s) 144, 192, 474
BstMBI GATC 2 cut(s) 406, 667
BstNI CCWGG 1 cut(s) 329
BstSCI CCNGG 2 cut(s) 327, 402
BstSFI CTRYAG 1 cut(s) 921
BstSLI GKGCMC 1 cut(s) 742
BstV1I GCAGC 8 cut(s) 662, 663, 666, 823, 907, 930, 974, 1008
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BstZ17I GTATAC 1 cut(s) 988
Bsu36I CCTNAGG 1 cut(s) 948
BsuRI GGCC 1 cut(s) 309
BtgI CCRYGG 1 cut(s) 1110
BtsCI GGATG 1 cut(s) 101
BtsI GCAGTG 1 cut(s) 173
BtsIMutI CAGTG 3 cut(s) 173, 740, 756
CfoI GCGC 1 cut(s) 496
CseI GACGC 1 cut(s) 847
CspCI CAANNNNNGTGG 2 cut(s) 15, 50
CviAII CATG 4 cut(s) 22, 809, 1100, 1111
DdeI CTNAG 8 cut(s) 212, 397, 743, 748, 948, 1067, 1104, 1131
DpnI GATC 2 cut(s) 408, 669
DpnII GATC 2 cut(s) 406, 667
Eam1104I CTCTTC 4 cut(s) 379, 777, 1005, 1029
EarI CTCTTC 4 cut(s) 379, 777, 1005, 1029
Ecl136II GAGCTC 1 cut(s) 747
Eco130I CCWWGG 1 cut(s) 1110
Eco24I GRGCYC 1 cut(s) 749
Eco53kI GAGCTC 1 cut(s) 747
Eco57I CTGAAG 1 cut(s) 767
Eco72I CACGTG 1 cut(s) 29
Eco81I CCTNAGG 1 cut(s) 948
EcoICRI GAGCTC 1 cut(s) 747
EcoRI GAATTC 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 327
EcoT14I CCWWGG 1 cut(s) 1110
EcoT22I ATGCAT 1 cut(s) 664
EcoT38I GRGCYC 1 cut(s) 749
ErhI CCWWGG 1 cut(s) 1110
Esp3I CGTCTC 1 cut(s) 192
FaeI CATG 4 cut(s) 25, 812, 1103, 1114
FatI CATG 4 cut(s) 21, 808, 1099, 1110
FauI CCCGC 1 cut(s) 1017
FbaI TGATCA 1 cut(s) 667
FblI GTMKAC 1 cut(s) 987
FokI GGATG 1 cut(s) 88
FriOI GRGCYC 1 cut(s) 749
FspBI CTAG 3 cut(s) 54, 527, 819
GlaI GCGC 1 cut(s) 495
HaeIII GGCC 1 cut(s) 309
HapII CCGG 3 cut(s) 245, 404, 1046
HgaI GACGC 1 cut(s) 847
HhaI GCGC 1 cut(s) 496
Hin1II CATG 4 cut(s) 25, 812, 1103, 1114
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HincII GTYRAC 1 cut(s) 1092
HindII GTYRAC 1 cut(s) 1092
HindIII AAGCTT 2 cut(s) 788, 1069
HinfI GANTC 8 cut(s) 46, 382, 415, 440, 467, 565, 644, 822
HpaII CCGG 3 cut(s) 245, 404, 1046
HphI GGTGA 5 cut(s) 7, 41, 466, 641, 922
Hpy166II GTNNAC 4 cut(s) 61, 638, 988, 1092
Hpy188III TCNNGA 5 cut(s) 34, 286, 412, 586, 704
Hpy8I GTNNAC 4 cut(s) 61, 638, 988, 1092
Hpy99I CGWCG 1 cut(s) 863
HpyAV CCTTC 5 cut(s) 28, 451, 521, 872, 880
HpyCH4IV ACGT 2 cut(s) 28, 712
HpyCH4V TGCA 5 cut(s) 344, 662, 682, 757, 923
HpyF3I CTNAG 8 cut(s) 212, 397, 743, 748, 948, 1067, 1104, 1131
HpySE526I ACGT 2 cut(s) 28, 712
Hsp92II CATG 4 cut(s) 25, 812, 1103, 1114
HspAI GCGC 1 cut(s) 494
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 2 cut(s) 406, 667
LmnI GCTCC 2 cut(s) 337, 523
Lsp1109I GCAGC 8 cut(s) 662, 663, 666, 823, 907, 930, 974, 1008
LweI GCATC 3 cut(s) 343, 611, 860
MaeI CTAG 3 cut(s) 54, 527, 819
MaeII ACGT 2 cut(s) 28, 712
MalI GATC 2 cut(s) 408, 669
MboI GATC 2 cut(s) 406, 667
MboII GAAGA 9 cut(s) 366, 574, 597, 607, 737, 764, 1022, 1046, 1119
MfeI CAATTG 2 cut(s) 671, 683
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 2 cut(s) 742, 749
MluCI AATT 7 cut(s) 67, 302, 671, 683, 972, 1084, 1119
MlyI GAGTC 4 cut(s) 40, 476, 653, 831
MmeI TCCRAC 1 cut(s) 633
Mph1103I ATGCAT 1 cut(s) 664
MseI TTAA 1 cut(s) 581
MslI CAYNNNNRTG 2 cut(s) 20, 178
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 3 cut(s) 245, 404, 1046
MspR9I CCNGG 2 cut(s) 329, 404
MunI CAATTG 2 cut(s) 671, 683
MvaI CCWGG 1 cut(s) 329
NciI CCSGG 1 cut(s) 404
NcoI CCATGG 1 cut(s) 1110
NdeII GATC 2 cut(s) 406, 667
NlaIII CATG 4 cut(s) 25, 812, 1103, 1114
NlaIV GGNNCC 2 cut(s) 333, 519
NsiI ATGCAT 1 cut(s) 664
OliI CACNNNNGTG 1 cut(s) 178
PcsI WCGNNNNNNNCGW 1 cut(s) 855
PfeI GAWTC 4 cut(s) 382, 415, 440, 565
PleI GAGTC 4 cut(s) 40, 475, 652, 830
PmaCI CACGTG 1 cut(s) 29
PmlI CACGTG 1 cut(s) 29
PpsI GAGTC 4 cut(s) 40, 475, 652, 830
Ppu21I YACGTR 1 cut(s) 29
Psp124BI GAGCTC 1 cut(s) 749
Psp1406I AACGTT 1 cut(s) 712
Psp6I CCWGG 1 cut(s) 327
PspCI CACGTG 1 cut(s) 29
PspGI CCWGG 1 cut(s) 327
PspN4I GGNNCC 2 cut(s) 333, 519
PstI CTGCAG 1 cut(s) 925
PsuI RGATCY 1 cut(s) 406
RseI CAYNNNNRTG 2 cut(s) 20, 178
SacI GAGCTC 1 cut(s) 749
SaqAI TTAA 1 cut(s) 581
Sau3AI GATC 2 cut(s) 406, 667
SchI GAGTC 4 cut(s) 40, 476, 653, 831
ScrFI CCNGG 2 cut(s) 329, 404
SduI GDGCHC 2 cut(s) 742, 749
SfaNI GCATC 3 cut(s) 343, 611, 860
SfcI CTRYAG 1 cut(s) 921
SmiMI CAYNNNNRTG 2 cut(s) 20, 178
SmlI CTYRAG 3 cut(s) 410, 641, 702
SmoI CTYRAG 3 cut(s) 410, 641, 702
Sse9I AATT 7 cut(s) 67, 302, 671, 683, 972, 1084, 1119
SsiI CCGC 6 cut(s) 335, 348, 357, 621, 648, 1024
SspMI CTAG 3 cut(s) 54, 527, 819
SstI GAGCTC 1 cut(s) 749
StyD4I CCNGG 2 cut(s) 327, 402
StyI CCWWGG 1 cut(s) 1110
TaiI ACGT 2 cut(s) 31, 715
TaqI TCGA 5 cut(s) 118, 222, 363, 593, 793
TasI AATT 7 cut(s) 67, 302, 671, 683, 972, 1084, 1119
TauI GCSGC 2 cut(s) 359, 623
TfiI GAWTC 4 cut(s) 382, 415, 440, 565
Tru1I TTAA 1 cut(s) 581
Tru9I TTAA 1 cut(s) 581
TscAI CASTG 3 cut(s) 180, 747, 756
TseI GCWGC 8 cut(s) 650, 676, 679, 836, 920, 943, 962, 1021
TspDTI ATGAA 6 cut(s) 10, 101, 575, 899, 1023, 1047
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 3 cut(s) 180, 747, 756
XapI RAATTY 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 987
XspI CTAG 3 cut(s) 54, 527, 819
Zsp2I ATGCAT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.