pycom16g14540

acyl-CoA-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010980
Physical Location & Seq
Reverse (-)
10612305 .. 10617425
5121 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2307 bp
ATGTTCGAAGATAAAAACAAGTACTTGCAAGGATTTTCATCTTTAAGTAAAAAAACAAAAGGAAATTCCTGTGATGCGCTACAGGACAACCTGCGCCAAGATGCTAACGTACGAAAGGAAGAGGTTTGTTCCTGCTGCGGAGGTTGCTGTTTCGCGAAGATGTTCGGTTTTTCTCGCAGGCGCATGAAGTTTGGACGGTTGAAAGTCCAGCGTTCGGATTCTGCTCAGGGAACCAGAAGCCCCATAAGAAACCCGAAACGAAATAACAGCTCCAATAATGAATCTGATGTGCCGGCAAGTAGTAATTCTGATGAGCTCGATGGTCAGTGTCCATCTGCTGTGCCCGAGATTAGTAATTCCGCGACTGGGAGCTCTGAGAATTGGATGGTGTTGTCGATTTCTGGGGACAAGCCTACACCCCGCTTCAATCATGCGGCTGCTGTAATTGGGAACAAAATGATAGTTGTTGGTGGTGAATCTGGGAATGAATTGCTAAATGATGTACAGGTGCTTAACTTTGATAGTTTGACGTGGACCACAGCTTCGTCAAAGCTTTACTTGTCACCGAGCAGTCTGCCATTAAAGATCCCTGCATGCAAGGGCCACAAACTGGTGTCTTGGGGGAAAAAGGCACTCCTCATTGGAGGAAAAACTGAACCGTGGAGCGACAAAATTTCAGTTTGGGCATTTGATACAGAGACAGAATGCTGGTCTCATATGGAAGCAAAGGGCAATATACCGGTTGCTCGCACTGGTCACACTGTAGTCAGGGCAAGCTCTACATTAATCCTATTTGGAGGTGAAGATGCTAAAAGGAGGAAATTGAACGATTTGCATATGTTTGATCTGAAGTCGTTGACATGGTTACCCCTTCATTGCACCGGTACAGGACCATCTGCAAGGTCCAACCACGTAGCAGCTCTTTATGATGATAAAACTCTTTTAATATTTGGAGGCACATCAAAATCCAAGACTTTGAATGATTTGTATTCAATGGACTTTGAAACTATGGAATGGTCAAGAATAAAGAAACGAGGTTTCCATCCATCTCCTAGAGCTGGTTGCTGTGGGGTCCTATGTGGAACCAAATGGTATATCGTTGGGGGTGGAAGCAGGAAAAAACGACATGCTGAGACTCTGATCTTTGATATCTGGAAACTTGAGTGGTCAGTGGCCATTACATCGCCTCCTTCTTCAATCACCGCGAATAAGGGTTTCAGCCTAGGGCTCGTGCAACACAAAGAAAAGGATTTTCTTGTTTCTTTTGGGGGATGCAGAAAGGAACCAACGAATCAGGTTGAAGTACTGACCATGGAAAAGAATGAATCATCCATAAGTCGTCGATCTTTACCCACCAAAGGCCCTGGAACTTTGCTGCCTGAGAAACGCGCATCATCCACAGGCTTGGCTTCTCAACTAAGTAATGGTTCTTCCCAACGCTCAGTTAATTCGGTTACCAGACAAAATCTTGCATCTGCAATTGAACAACATGGTTCTGGAAGAAAATCTTTGTCGGAGTCCTTGCTTGTTGATCCAAATACTGTTTCTGGTAACGTTTCACTCAGGAAACAATTCCACAATGAAGAAGAATTCAACACGACTGTTAAGGTGGCAAAAAGTACGGAAGATGAAAGTTCTTTCTCGCAGCCAATAGAAAAGAAAACCAGTCAATCTGATTCAGGAGTCCAGACTAACATATATGGAGGCAGACTCTTTTCGGAGGAGATACTATCTGGATATGATTTTGAAAATTCAAATCCTCACCATCATGGAACTGGAAACCTTTCAATGGATAACAACGATGCCGTGTTCCCAGAAAATGATAGTAAACTAGGAGCCTTATCAACTGCTTCAAGTATGTACCACTACTATGAATCAAAGATGGCCGCACTAATAAGAAAGAATGGAATTTTAGAAGGAAAGTTAGCTACTGTGATGGCCAGCCAGGAAGCTGCTGAGAAAAATTTATCCTCGGCCCTCAAGAGCAGACAGGAGATGGAGAGAAAATTGTCAGACAGTGCGAAGGAGATGGAATTTCTGAAGGAAAAGGTTGCGGGTGTAGAGATAGCGCAAGAAGAGGCTAACAACCTCTCAAATATTGTTCATTCTGACAATGTCAGGCTTGAGCATGATGTGGCTTTCCTCAAGGCGGTTTTGGATGATACCCAGAAGGAGCTCCATTCAACCAGAGGAGTCCTTGCAGGAGAACGAGCAAGGGCATTCCAGCTACAGGTTGAAGTTTTCCACCTGAAACAGAGATTGCAATCTATGGAGAACCGAGCACCGACACCTCGGAAACCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1 Kelch_4 Kelch_5

Protein Analysis

769

Amino Acids

84.51

Weight (kDa)

8.93

Isoelectric Point (pI)

49.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 124 - 256 3.1e-13 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 125 - 182 1.1e-12 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 125 - 184 5.5e-11 FBX42, beta-propeller domain
Kelch_HCF PF13854 129 - 371 1.7e-24 Host cell factor, Kelch-repeats domain
Kelch_1 PF01344 140 - 183 3.4e-09 Kelch motif
Beta-prop_FBX42 PF13415 199 - 323 1.4e-24 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 201 - 424 4.1e-59 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 213 - 346 1.7e-21 Attractin/LZTR1 beta-propeller
Beta-prop_TYW4 PF13418 247 - 446 3.9e-09 tRNA wybutosine-synthesizing protein 4, beta-propeller
Beta-prop_ATRN-LZTR1 PF24981 263 - 434 2.2e-20 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 316 - 444 2.3e-15 FBX42, beta-propeller domain
ACBP4_C PF24922 698 - 758 1.7e-09 Acyl-CoA-binding domain-containing protein 4-6, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18590 AT5G18590
fragaria_vesca FvH4_4g17510 FvH4_4g17510 FvH4_4g17510
malus_domestica MD16G1173400.v1.1
prunus_persica Prupe.1G140300_v2.0.a1 Prupe.1G140300_v2.0.a1
pyrus_communis pycom16g14540
rosa_chinensis RchiOBHm_Chr4g0421431
rosa_laevigata RLG00000007685
rosa_multiflora Rmu_sc0001962.1_g000012
rosa_roxburghii Rroxscaffold_5G00363700
rosa_rugosa Rorug04G0171000 Rorug04G0171100
rosa_samantha Rh4AG231800 Rh4BG234700 Rh4CG246300 Rh4DG230300
rosa_wichuraiana Rw4G020230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 99
AccB7I CCANNNNNTGG 1 cut(s) 610
AccII CGCG 4 cut(s) 155, 362, 1205, 1389
AciI CCGC 8 cut(s) 138, 360, 421, 434, 1203, 1887, 2054, 2150
AclI AACGTT 1 cut(s) 1554
AclWI GGATC 2 cut(s) 580, 1526
AcoI YGGCCR 3 cut(s) 1173, 1884, 1938
AcsI RAATTY 7 cut(s) 64, 672, 1589, 1750, 1908, 1963, 2033
AcuI CTGAAG 2 cut(s) 869, 2060
AfaI GTAC 7 cut(s) 23, 111, 504, 886, 1305, 1621, 1862
AfiI CCNNNNNNNGG 8 cut(s) 214, 366, 610, 1058, 1358, 1789, 2149, 2230
AgeI ACCGGT 2 cut(s) 739, 881
AjiI CACGTC 1 cut(s) 531
AjnI CCWGG 2 cut(s) 1363, 1944
AjuI GAANNNNNNNTTGG 2 cut(s) 1528, 1560
Alw21I GWGCWC 4 cut(s) 318, 374, 2178, 2284
Alw26I GTCTC 3 cut(s) 692, 717, 1127
AlwI GGATC 2 cut(s) 580, 1526
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 6 cut(s) 601, 1173, 1360, 1884, 1938, 1974
ApeKI GCWGC 6 cut(s) 135, 437, 917, 1375, 1645, 1952
ApoI RAATTY 7 cut(s) 64, 672, 1589, 1750, 1908, 1963, 2033
ArsI GACNNNNNNTTYG 2 cut(s) 526, 558
AseI ATTAAT 1 cut(s) 785
AsiGI ACCGGT 2 cut(s) 739, 881
Asp700I GAANNNNTTC 4 cut(s) 161, 1571, 1783, 2300
AspA2I CCTAGG 1 cut(s) 1222
AspLEI GCGC 5 cut(s) 79, 96, 183, 1391, 2071
AspS9I GGNCC 7 cut(s) 534, 601, 890, 903, 1072, 1361, 1975
AsuHPI GGTGA 5 cut(s) 485, 555, 812, 1192, 1754
AsuII TTCGAA 1 cut(s) 6
AvaI CYCGRG 1 cut(s) 344
AvaII GGWCC 4 cut(s) 534, 890, 903, 1072
AvrII CCTAGG 1 cut(s) 1222
BaeGI GKGCMC 1 cut(s) 345
BalI TGGCCA 2 cut(s) 1175, 1940
BanII GRGCYC 4 cut(s) 318, 374, 1230, 2178
BarI GAAGNNNNNNTAC 2 cut(s) 1414, 1446
BauI CACGAG 1 cut(s) 1229
Bbv12I GWGCWC 4 cut(s) 318, 374, 2178, 2284
BbvI GCAGC 6 cut(s) 122, 424, 929, 1362, 1657, 1939
BceAI ACGGC 1 cut(s) 1790
BcgI CGANNNNNNTGC 2 cut(s) 556, 590
BciT130I CCWGG 2 cut(s) 1365, 1946
BcoDI GTCTC 3 cut(s) 692, 717, 1127
BfaI CTAG 4 cut(s) 1053, 1223, 1832, 2305
BfmI CTRYAG 3 cut(s) 80, 762, 2228
BfuAI ACCTGC 1 cut(s) 99
BisI GCNGC 8 cut(s) 136, 435, 438, 918, 1376, 1646, 1887, 1953
BlnI CCTAGG 1 cut(s) 1222
BlsI GCNGC 8 cut(s) 137, 436, 439, 919, 1377, 1647, 1888, 1954
BmcAI AGTACT 2 cut(s) 23, 1305
Bme1390I CCNGG 2 cut(s) 1365, 1946
Bme18I GGWCC 4 cut(s) 534, 890, 903, 1072
BmeT110I CYCGRG 1 cut(s) 344
BmgBI CACGTC 1 cut(s) 531
BmgT120I GGNCC 7 cut(s) 534, 601, 890, 903, 1072, 1361, 1975
BmiI GGNNCC 5 cut(s) 232, 1073, 1084, 1284, 1837
BmrFI CCNGG 2 cut(s) 1365, 1946
BmrI ACTGGG 1 cut(s) 375
BmsI GCATC 7 cut(s) 64, 91, 796, 1262, 1400, 1481, 1792
BmuI ACTGGG 1 cut(s) 375
BplI GAGNNNNNCTC 2 cut(s) 1695, 1727
Bpu10I CCTNAGC 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 6
BpuEI CTTGAG 4 cut(s) 1181, 1964, 2129, 2144
BsaAI YACGTR 1 cut(s) 913
BsaBI GATNNNNATC 2 cut(s) 37, 2263
BsaI GGTCTC 1 cut(s) 717
BsaJI CCNNGG 6 cut(s) 659, 1222, 1311, 1363, 1971, 2291
BsaWI WCCGGW 2 cut(s) 739, 881
BsaXI ACNNNNNCTCC 2 cut(s) 1171, 1201
Bsc4I CCNNNNNNNGG 8 cut(s) 214, 366, 610, 1058, 1358, 1789, 2149, 2230
Bse118I RCCGGY 3 cut(s) 292, 739, 881
Bse1I ACTGG 5 cut(s) 370, 615, 757, 1665, 1780
Bse3DI GCAATG 1 cut(s) 874
Bse8I GATNNNNATC 2 cut(s) 37, 2263
BseBI CCWGG 2 cut(s) 1365, 1946
BseDI CCNNGG 6 cut(s) 659, 1222, 1311, 1363, 1971, 2291
BseGI GGATG 6 cut(s) 390, 1042, 1277, 1328, 1394, 2164
BseJI GATNNNNATC 2 cut(s) 37, 2263
BseLI CCNNNNNNNGG 8 cut(s) 214, 366, 610, 1058, 1358, 1789, 2149, 2230
BseMI GCAATG 1 cut(s) 874
BseMII CTCAG 7 cut(s) 239, 366, 1122, 1371, 1455, 1576, 1947
BseNI ACTGG 5 cut(s) 370, 615, 757, 1665, 1780
BseRI GAGGAG 3 cut(s) 626, 1736, 2205
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 6 cut(s) 122, 424, 929, 1362, 1657, 1939
Bsh1236I CGCG 4 cut(s) 155, 362, 1205, 1389
BshFI GGCC 6 cut(s) 603, 1175, 1362, 1886, 1940, 1976
BshTI ACCGGT 2 cut(s) 739, 881
BsiHKAI GWGCWC 4 cut(s) 318, 374, 2178, 2284
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 3 cut(s) 293, 740, 882
BsiWI CGTACG 1 cut(s) 109
BslFI GGGAC 1 cut(s) 419
BslI CCNNNNNNNGG 8 cut(s) 214, 366, 610, 1058, 1358, 1789, 2149, 2230
BsmAI GTCTC 3 cut(s) 692, 717, 1127
BsmFI GGGAC 1 cut(s) 419
BsmI GAATGC 2 cut(s) 710, 2219
BsnI GGCC 6 cut(s) 603, 1175, 1362, 1886, 1940, 1976
Bso31I GGTCTC 1 cut(s) 717
BsoBI CYCGRG 1 cut(s) 344
Bsp119I TTCGAA 1 cut(s) 6
Bsp1286I GDGCHC 6 cut(s) 318, 345, 374, 1230, 2178, 2284
Bsp1407I TGTACA 1 cut(s) 502
Bsp143I GATC 5 cut(s) 585, 844, 1140, 1343, 1531
Bsp19I CCATGG 1 cut(s) 1311
Bsp68I TCGCGA 1 cut(s) 155
BspACI CCGC 8 cut(s) 138, 360, 421, 434, 1203, 1887, 2054, 2150
BspANI GGCC 6 cut(s) 603, 1175, 1362, 1886, 1940, 1976
BspCNI CTCAG 7 cut(s) 238, 367, 1123, 1372, 1454, 1575, 1948
BspFNI CGCG 4 cut(s) 155, 362, 1205, 1389
BspLI GGNNCC 5 cut(s) 232, 1073, 1084, 1284, 1837
BspMI ACCTGC 1 cut(s) 99
BspPI GGATC 2 cut(s) 580, 1526
BspT104I TTCGAA 1 cut(s) 6
BspTNI GGTCTC 1 cut(s) 717
BsrDI GCAATG 1 cut(s) 874
BsrFI RCCGGY 3 cut(s) 292, 739, 881
BsrGI TGTACA 1 cut(s) 502
BsrI ACTGG 5 cut(s) 370, 615, 757, 1665, 1780
BssAI RCCGGY 3 cut(s) 292, 739, 881
BssECI CCNNGG 6 cut(s) 659, 1222, 1311, 1363, 1971, 2291
BssMI GATC 5 cut(s) 585, 844, 1140, 1343, 1531
BssSI CACGAG 1 cut(s) 1229
BssT1I CCWWGG 2 cut(s) 1222, 1311
Bst2BI CACGAG 1 cut(s) 1229
Bst2UI CCWGG 2 cut(s) 1365, 1946
Bst4CI ACNGT 7 cut(s) 198, 660, 763, 1543, 1603, 1933, 2018
Bst6I CTCTTC 2 cut(s) 114, 2070
BstAUI TGTACA 1 cut(s) 502
BstBAI YACGTR 1 cut(s) 913
BstBI TTCGAA 1 cut(s) 6
BstC8I GCNNGC 6 cut(s) 179, 294, 595, 748, 775, 1942
BstDEI CTNAG 8 cut(s) 225, 375, 1131, 1380, 1418, 1441, 1562, 1956
BstDSI CCRYGG 2 cut(s) 659, 1311
BstEII GGTNACC 2 cut(s) 864, 1453
BstF5I GGATG 6 cut(s) 390, 1042, 1277, 1328, 1394, 2164
BstFNI CGCG 4 cut(s) 155, 362, 1205, 1389
BstHHI GCGC 5 cut(s) 79, 96, 183, 1391, 2071
BstKTI GATC 5 cut(s) 588, 847, 1143, 1346, 1534
BstMAI GTCTC 3 cut(s) 692, 717, 1127
BstMBI GATC 5 cut(s) 585, 844, 1140, 1343, 1531
BstMWI GCNNNNNNNGC 1 cut(s) 144
BstNI CCWGG 2 cut(s) 1365, 1946
BstNSI RCATGY 2 cut(s) 597, 1130
BstPI GGTNACC 2 cut(s) 864, 1453
BstSCI CCNGG 2 cut(s) 1363, 1944
BstSFI CTRYAG 3 cut(s) 80, 762, 2228
BstSLI GKGCMC 1 cut(s) 345
BstUI CGCG 4 cut(s) 155, 362, 1205, 1389
BstV1I GCAGC 6 cut(s) 122, 424, 929, 1362, 1657, 1939
BstX2I RGATCY 1 cut(s) 585
BstXI CCANNNNNNTGG 1 cut(s) 1405
BstYI RGATCY 1 cut(s) 585
BsuRI GGCC 6 cut(s) 603, 1175, 1362, 1886, 1940, 1976
BtgI CCRYGG 2 cut(s) 659, 1311
BtgZI GCGATG 1 cut(s) 1167
BtrI CACGTC 1 cut(s) 531
BtsCI GGATG 6 cut(s) 390, 1042, 1277, 1328, 1394, 2164
BtsIMutI CAGTG 5 cut(s) 332, 750, 759, 1176, 2023
BtuMI TCGCGA 1 cut(s) 155
BveI ACCTGC 1 cut(s) 99
Cac8I GCNNGC 6 cut(s) 179, 294, 595, 748, 775, 1942
CfoI GCGC 5 cut(s) 79, 96, 183, 1391, 2071
Cfr10I RCCGGY 3 cut(s) 292, 739, 881
Cfr13I GGNCC 7 cut(s) 534, 601, 890, 903, 1072, 1361, 1975
Csp6I GTAC 7 cut(s) 22, 110, 503, 885, 1304, 1620, 1861
CspAI ACCGGT 2 cut(s) 739, 881
CviAII CATG 9 cut(s) 184, 431, 594, 861, 1127, 1312, 1490, 1769, 2129
CviQI GTAC 7 cut(s) 22, 110, 503, 885, 1304, 1620, 1861
DdeI CTNAG 8 cut(s) 225, 375, 1131, 1380, 1418, 1441, 1562, 1956
DpnI GATC 5 cut(s) 587, 846, 1142, 1345, 1533
DpnII GATC 5 cut(s) 585, 844, 1140, 1343, 1531
EaeI YGGCCR 3 cut(s) 1173, 1884, 1938
Eam1104I CTCTTC 2 cut(s) 114, 2070
EarI CTCTTC 2 cut(s) 114, 2070
Ecl136II GAGCTC 3 cut(s) 316, 372, 2176
Eco130I CCWWGG 2 cut(s) 1222, 1311
Eco24I GRGCYC 4 cut(s) 318, 374, 1230, 2178
Eco31I GGTCTC 1 cut(s) 717
Eco32I GATATC 1 cut(s) 1150
Eco47I GGWCC 4 cut(s) 534, 890, 903, 1072
Eco53kI GAGCTC 3 cut(s) 316, 372, 2176
Eco57I CTGAAG 2 cut(s) 869, 2060
Eco88I CYCGRG 1 cut(s) 344
Eco91I GGTNACC 2 cut(s) 864, 1453
EcoICRI GAGCTC 3 cut(s) 316, 372, 2176
EcoO109I RGGNCCY 2 cut(s) 1072, 1361
EcoO65I GGTNACC 2 cut(s) 864, 1453
EcoRI GAATTC 1 cut(s) 1589
EcoRII CCWGG 2 cut(s) 1363, 1944
EcoRV GATATC 1 cut(s) 1150
EcoT14I CCWWGG 2 cut(s) 1222, 1311
EcoT38I GRGCYC 4 cut(s) 318, 374, 1230, 2178
ErhI CCWWGG 2 cut(s) 1222, 1311
FaeI CATG 9 cut(s) 187, 434, 597, 864, 1130, 1315, 1493, 1772, 2132
FalI AAGNNNNNCTT 4 cut(s) 542, 574, 1492, 1524
FaqI GGGAC 1 cut(s) 419
FatI CATG 9 cut(s) 183, 430, 593, 860, 1126, 1311, 1489, 1768, 2128
FauI CCCGC 2 cut(s) 428, 2047
FauNDI CATATG 2 cut(s) 717, 837
Fnu4HI GCNGC 8 cut(s) 136, 435, 438, 918, 1376, 1646, 1887, 1953
FokI GGATG 6 cut(s) 397, 1029, 1284, 1315, 1381, 2171
FriOI GRGCYC 4 cut(s) 318, 374, 1230, 2178
Fsp4HI GCNGC 8 cut(s) 136, 435, 438, 918, 1376, 1646, 1887, 1953
FspBI CTAG 4 cut(s) 1053, 1223, 1832, 2305
GlaI GCGC 5 cut(s) 78, 95, 182, 1390, 2070
GluI GCNGC 8 cut(s) 136, 435, 438, 918, 1376, 1646, 1887, 1953
HaeIII GGCC 6 cut(s) 603, 1175, 1362, 1886, 1940, 1976
HapII CCGG 3 cut(s) 293, 740, 882
HhaI GCGC 5 cut(s) 79, 96, 183, 1391, 2071
Hin1II CATG 9 cut(s) 187, 434, 597, 864, 1130, 1315, 1493, 1772, 2132
Hin6I GCGC 5 cut(s) 77, 94, 181, 1389, 2069
HinP1I GCGC 5 cut(s) 77, 94, 181, 1389, 2069
HincII GTYRAC 1 cut(s) 858
HindII GTYRAC 1 cut(s) 858
HindIII AAGCTT 1 cut(s) 551
HpaII CCGG 3 cut(s) 293, 740, 882
HphI GGTGA 5 cut(s) 485, 555, 812, 1192, 1754
Hpy166II GTNNAC 3 cut(s) 534, 858, 1829
Hpy188III TCNNGA 9 cut(s) 154, 1020, 1153, 1497, 1564, 1680, 1687, 1734, 1981
Hpy8I GTNNAC 3 cut(s) 534, 858, 1829
Hpy99I CGWCG 1 cut(s) 1344
HpyAV CCTTC 6 cut(s) 881, 1200, 1910, 2017, 2035, 2164
HpyCH4III ACNGT 7 cut(s) 198, 660, 763, 1543, 1603, 1933, 2018
HpyCH4IV ACGT 4 cut(s) 108, 530, 912, 1554
HpyF10VI GCNNNNNNNGC 1 cut(s) 144
HpyF3I CTNAG 8 cut(s) 225, 375, 1131, 1380, 1418, 1441, 1562, 1956
HpySE526I ACGT 4 cut(s) 108, 530, 912, 1554
Hsp92II CATG 9 cut(s) 187, 434, 597, 864, 1130, 1315, 1493, 1772, 2132
HspAI GCGC 5 cut(s) 77, 94, 181, 1389, 2069
KroI GCCGGC 1 cut(s) 292
KroNI GCCGGC 1 cut(s) 294
Kzo9I GATC 5 cut(s) 585, 844, 1140, 1343, 1531
LmnI GCTCC 6 cut(s) 275, 369, 663, 1835, 2173, 2181
Lsp1109I GCAGC 6 cut(s) 122, 424, 929, 1362, 1657, 1939
LweI GCATC 7 cut(s) 64, 91, 796, 1262, 1400, 1481, 1792
MaeI CTAG 4 cut(s) 1053, 1223, 1832, 2305
MaeII ACGT 4 cut(s) 108, 530, 912, 1554
MaeIII GTNAC 5 cut(s) 561, 755, 864, 1453, 1550
MalI GATC 5 cut(s) 587, 846, 1142, 1345, 1533
MboI GATC 5 cut(s) 585, 844, 1140, 1343, 1531
MfeI CAATTG 1 cut(s) 1479
MflI RGATCY 1 cut(s) 585
MhlI GDGCHC 6 cut(s) 318, 345, 374, 1230, 2178, 2284
MlsI TGGCCA 2 cut(s) 1175, 1940
MluNI TGGCCA 2 cut(s) 1175, 1940
MlyI GAGTC 5 cut(s) 1129, 1526, 1692, 1704, 2202
MmeI TCCRAC 2 cut(s) 930, 1494
Mox20I TGGCCA 2 cut(s) 1175, 1940
MroNI GCCGGC 1 cut(s) 292
MroXI GAANNNNTTC 4 cut(s) 161, 1571, 1783, 2300
MscI TGGCCA 2 cut(s) 1175, 1940
MseI TTAA 7 cut(s) 44, 513, 581, 785, 944, 1446, 1605
MslI CAYNNNNRTG 2 cut(s) 1767, 1869
Msp20I TGGCCA 2 cut(s) 1175, 1940
MspI CCGG 3 cut(s) 293, 740, 882
MspR9I CCNGG 2 cut(s) 1365, 1946
MunI CAATTG 1 cut(s) 1479
Mva1269I GAATGC 2 cut(s) 710, 2219
MvaI CCWGG 2 cut(s) 1365, 1946
MvnI CGCG 4 cut(s) 155, 362, 1205, 1389
MwoI GCNNNNNNNGC 1 cut(s) 144
NaeI GCCGGC 1 cut(s) 294
NcoI CCATGG 1 cut(s) 1311
NdeI CATATG 2 cut(s) 717, 837
NdeII GATC 5 cut(s) 585, 844, 1140, 1343, 1531
NgoMIV GCCGGC 1 cut(s) 292
NlaIII CATG 9 cut(s) 187, 434, 597, 864, 1130, 1315, 1493, 1772, 2132
NlaIV GGNNCC 5 cut(s) 232, 1073, 1084, 1284, 1837
NmeAIII GCCGAG 1 cut(s) 1952
NmuCI GTSAC 2 cut(s) 561, 755
NruI TCGCGA 1 cut(s) 155
NspI RCATGY 2 cut(s) 597, 1130
NspV TTCGAA 1 cut(s) 6
PaeI GCATGC 1 cut(s) 597
PctI GAATGC 2 cut(s) 710, 2219
PdiI GCCGGC 1 cut(s) 294
PdmI GAANNNNTTC 4 cut(s) 161, 1571, 1783, 2300
PfeI GAWTC 7 cut(s) 218, 281, 476, 1291, 1325, 1676, 1874
Pfl23II CGTACG 1 cut(s) 109
PflFI GACNNNGTC 1 cut(s) 2114
PflMI CCANNNNNTGG 1 cut(s) 610
PinAI ACCGGT 2 cut(s) 739, 881
PkrI GCNGC 8 cut(s) 137, 436, 439, 919, 1377, 1647, 1888, 1954
PleI GAGTC 5 cut(s) 1129, 1525, 1691, 1704, 2201
PpsI GAGTC 5 cut(s) 1129, 1525, 1691, 1704, 2201
Ppu21I YACGTR 1 cut(s) 913
PpuMI RGGWCCY 1 cut(s) 1072
PshBI ATTAAT 1 cut(s) 785
Psp124BI GAGCTC 3 cut(s) 318, 374, 2178
Psp1406I AACGTT 1 cut(s) 1554
Psp5II RGGWCCY 1 cut(s) 1072
Psp6I CCWGG 2 cut(s) 1363, 1944
PspEI GGTNACC 2 cut(s) 864, 1453
PspGI CCWGG 2 cut(s) 1363, 1944
PspLI CGTACG 1 cut(s) 109
PspN4I GGNNCC 5 cut(s) 232, 1073, 1084, 1284, 1837
PspPI GGNCC 7 cut(s) 534, 601, 890, 903, 1072, 1361, 1975
PspPPI RGGWCCY 1 cut(s) 1072
PsuI RGATCY 1 cut(s) 585
PsyI GACNNNGTC 1 cut(s) 2114
RruI TCGCGA 1 cut(s) 155
RsaI GTAC 7 cut(s) 23, 111, 504, 886, 1305, 1621, 1862
RsaNI GTAC 7 cut(s) 22, 110, 503, 885, 1304, 1620, 1861
RseI CAYNNNNRTG 2 cut(s) 1767, 1869
SacI GAGCTC 3 cut(s) 318, 374, 2178
SaqAI TTAA 7 cut(s) 44, 513, 581, 785, 944, 1446, 1605
SatI GCNGC 8 cut(s) 136, 435, 438, 918, 1376, 1646, 1887, 1953
Sau3AI GATC 5 cut(s) 585, 844, 1140, 1343, 1531
Sau96I GGNCC 7 cut(s) 534, 601, 890, 903, 1072, 1361, 1975
ScaI AGTACT 2 cut(s) 23, 1305
SchI GAGTC 5 cut(s) 1129, 1526, 1692, 1704, 2202
ScrFI CCNGG 2 cut(s) 1365, 1946
SduI GDGCHC 6 cut(s) 318, 345, 374, 1230, 2178, 2284
SfaNI GCATC 7 cut(s) 64, 91, 796, 1262, 1400, 1481, 1792
SfcI CTRYAG 3 cut(s) 80, 762, 2228
SfuI TTCGAA 1 cut(s) 6
SinI GGWCC 4 cut(s) 534, 890, 903, 1072
SmiMI CAYNNNNRTG 2 cut(s) 1767, 1869
SmlI CTYRAG 4 cut(s) 1160, 1979, 2123, 2144
SmoI CTYRAG 4 cut(s) 1160, 1979, 2123, 2144
SphI GCATGC 1 cut(s) 597
SsiI CCGC 8 cut(s) 138, 360, 421, 434, 1203, 1887, 2054, 2150
SspI AATATT 2 cut(s) 948, 2098
SspMI CTAG 4 cut(s) 1053, 1223, 1832, 2305
SstI GAGCTC 3 cut(s) 318, 374, 2178
StyD4I CCNGG 2 cut(s) 1363, 1944
StyI CCWWGG 2 cut(s) 1222, 1311
TaaI ACNGT 7 cut(s) 198, 660, 763, 1543, 1603, 1933, 2018
TaiI ACGT 4 cut(s) 111, 533, 915, 1557
TaqI TCGA 4 cut(s) 6, 318, 395, 1342
TatI WGTACW 3 cut(s) 21, 502, 1303
TauI GCSGC 2 cut(s) 437, 1889
TfiI GAWTC 7 cut(s) 218, 281, 476, 1291, 1325, 1676, 1874
Tru1I TTAA 7 cut(s) 44, 513, 581, 785, 944, 1446, 1605
Tru9I TTAA 7 cut(s) 44, 513, 581, 785, 944, 1446, 1605
TscAI CASTG 5 cut(s) 332, 757, 766, 1176, 2023
TseFI GTSAC 2 cut(s) 561, 755
TseI GCWGC 6 cut(s) 135, 437, 917, 1375, 1645, 1952
Tsp45I GTSAC 2 cut(s) 561, 755
TspGWI ACGGA 1 cut(s) 1637
TspRI CASTG 5 cut(s) 332, 757, 766, 1176, 2023
Tth111I GACNNNGTC 1 cut(s) 2114
Van91I CCANNNNNTGG 1 cut(s) 610
VpaK11BI GGWCC 4 cut(s) 534, 890, 903, 1072
VspI ATTAAT 1 cut(s) 785
XapI RAATTY 7 cut(s) 64, 672, 1589, 1750, 1908, 1963, 2033
XceI RCATGY 2 cut(s) 597, 1130
XcmI CCANNNNNNNNNTGG 1 cut(s) 1772
XmaJI CCTAGG 1 cut(s) 1222
XmnI GAANNNNTTC 4 cut(s) 161, 1571, 1783, 2300
XspI CTAG 4 cut(s) 1053, 1223, 1832, 2305
ZrmI AGTACT 2 cut(s) 23, 1305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.