pycom16g18610
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007851
Physical Location & Seq
Forward (+)
15279666 .. 15283192
3527 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2895 bp
ATGTCGGCCACATCGGCTCGCCGCTTCAAAGACCGCGGCGGCGCCGGCGTGGCAGCAGGATCCAAACCCGCCGCCACTCTTAAACCATCCAAGCCTCTAACGCCGATACCCTTTTCTGACAAGAATTATGCCAGCGCCGGAAAGGAGAACCCCCTTCCAGGGACCGCTGCTTTCCGGGCTTCGGTCCAGAAGCCCACGATCCGGCCAGTTCCGCGAGTCGACAAGGCGGCTGTGTCTGCGGCAACCAGTAGCGCAGGTAGCGAAACGCGTTCCCGGTGGTCCATGTCATCGGCGCCGAGAGGTAGGAGCCCTAGCCCTTCTGAGTTTACGAGGGTAATTTCTCATACGGGTAAGGAGCGGAGGGTCTCGGTGGATCGGGTCAGACCGGGTTCGGGTCTGAGTTCGGTGGGGGAGAGGGATCGCGTAGTGTCGAGTGCAGGGAAGGTTAGAGGATCAGCTAATGGGAAGCAGAGAACATGGTTTAGGGATTTGGATGTGAAGAGGAGCGATGTGGGAGCGAATGGGATTAGGGTTTTGAGGGATATCAAAGAAAGTGGTAAAATTGGTGTGAATTTAGAGAAAAAGAATGCAACTTCTGGAGAATTAAAAGTGAGAGCGGTGGAAATTGAGAAGAATTCGGATGGGGTTAGGGTTAGGGATCCGGGAAGTGGAGATGGAGAAGCTAATTTGAGCTCCGATCTGAAAAACCCTAGTGCGGTTGATAAAAAGGATCAAAATTTGGCGAGGGTGGATGATAAAGCTGTGAAAATTGGGAGTGGTGATGCTTTGGGACTGAAGGAGTCTGGTGAAAAATCTGTAAGTGATGCAAAGGTTTTGGAGGGTTTTAAAGAAAAGAGGTTGAATGAAGAAGGTAGTAGTGGCGGTCGTTCGGGTATCAAATATCCGAGTAAGCTTCACGAGAAGCTTGCTTTCTTGGAAGGGAAGGTTAAGAGGATTGCCTCGGATATTAAGAAGACAAAGGAGATTTTGGATATGAATAATCCAGATACGTCGAAGGTGATACTCTCTGATATTCAGGAAAAGATTTCTGGAATTGAGAAGGCCATGAGACATGTCAATGATTCAGGTGGTAAGATGGGGTTGCCGAAAAGTACTGAGCATAGTGACAGGGATGCAAAAGTAGTTGAGAAGGGCCATATTGAGCTGGTGTGTAATGCTAAGAGTTTGGTAAAGGGGTTGAACAGTGAGGACTTGGAAGCTAGGCTCTTTCCTCATCATAAGTTGCTTCAAAACTGTGTGGCGTTGAAAGAATCATTAGAAAGCTCTCAAGGTCATGGGTTCCAAGCTGTTGAAACTAGTTGTGAAGCAAAGGTTGAAGAGGAGTCATTGAGCCTTACAGATGATAACCCAATTGCGGTAGAGTTCTTGGCTTCCTTGGACCAAACTAAAGTTTCAACAAGGGATGATGGACGTGAAGGTTTGGAATGTTTTGAAGTTCAAGAAGTGGATGGTGTCACTGCTGCAGAAGTTGAAAAATCTTCGAAATTTGTCTCTGGAAAACAAAATTTGGAGCTCATTCTCACAACCGATGAAAAACTTGATGAGTTTGATGATCAGGAGAATAGACAGGACATGTTCTTTGACGAGGAAAATGAGGATACTTGCAGTTACCAGCTGAACCAAATAGGCCAAAAAACCTCAACTGGAGGGTGGTTCATGTCTGAGGGAGAGTCGGTTCTTCTTGCTCATGATGATAGTTCGTGCACCTTCTATGATATTGTAAATTGTGAGGAGAAAGTTGTGTACAAGCCTCCTGCTGTAGTTTCACCTAATATGTGGAGAGACTGTTGGATTATCCGTGCTCCCAGTGCTGATGGTTGCTCAGGAAGGTATGTTGTGGCTGCATCAGCTGGCAATGCAATGGATGCAGGATTTTGTTCATGGGATTTCTATGCCAAAGATGTGCGTGCTTTCCGGATTGAGGATGGTTCAGCCCCTTCAAGAACAGTGCTTGGCCGCTTGCCCAACAACATCTCACATGGAAGAAATGCTTTGTCTGATCTTCTGGATCCAGAACCTAGACAATGGTGGTATAGACCTTGTGGACCTCTCATTGCCTCAACTGCTAGCTGTCAGAGAGTTGTGAGAATCTATGACATCCGTGATGGTGAGCAAGTTATGAAATGGGATGTATCAAAGCCTGTTATAGCAATGGATAATTCAAGCCCCTTGCAGTGGAGAAATAGAGGAAAAGTTGTTGTAGCTGAAGCTGAAACAATTTCCCTTTGGGATGTGAACTCTCTTAACCCTCAAGCTTTACTATCTGTTTCTTCTTCTGGTCGGAAAATTTCTGCTCTTCATGTGAAAAACACTGATGCTGAACTAGGTGGTGGTGTTCGACAAAGAGTAAGTTCTGCGGAAGCAGAAGGAAATGACGGAGTGTTCTGTACTCAAGATTCTATTAACATTATAGACTTTCGGCATCCTACTGGCGTAGGTCTTAAGATACCAAAACTCGGTGTAAATGTGCAATCAGTTTTCTCTCGTGGAGATTCTGTCTTCCTTGGCTGCACTAGTGCAAGATCGGGATGGAAAAAGCAGTCTTCTTCACAGGTGCAACAATTTTCAATCCGAAAACAAAGTCTATACAGCACCTACGCTTTGCCAGAATCAAATGCTCACAGTCATTACACAGAAATTACACAAGTTTGGGGGAATTCAAACCTTGTCATGGGTATTTGCGGATTGGGGCTGTTTGTATTTGATGCGTTGAAGGATGACGGAGTTCCATTGTTGACCAGTGATGACGGGAGTCAAAAAGCCAGAGAAACTGTTGGTCCAGATGACTTGTATGCACCTTCTTTTGATTACTTGGATTCTCGGGCACTTCTTATCTCAAGAGATCGCCCGGCACTGTGGAGGCGCCTATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

965

Amino Acids

104.97

Weight (kDa)

6.94

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_At4g14310 PF25465 678 - 959 4.8e-156 At4g14310 8-bladed propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 245
AccB1I GGYRCC 3 cut(s) 41, 292, 2883
AccBSI CCGCTC 2 cut(s) 358, 617
AccI GTMKAC 1 cut(s) 219
AccII CGCG 4 cut(s) 36, 214, 268, 423
AccIII TCCGGA 1 cut(s) 1935
AcoI YGGCCR 3 cut(s) 6, 203, 1975
AcsI RAATTY 7 cut(s) 571, 634, 736, 1505, 1525, 2307, 2677
AcuI CTGAAG 2 cut(s) 815, 2247
AcyI GRCGYC 3 cut(s) 42, 293, 2884
AfaI GTAC 3 cut(s) 1114, 1766, 2410
AflII CTTAAG 1 cut(s) 2462
AflIII ACRYGT 3 cut(s) 266, 1072, 1593
AhlI ACTAGT 2 cut(s) 1316, 2534
AjiI CACGTC 1 cut(s) 1433
AjnI CCWGG 1 cut(s) 157
AloI GAACNNNNNNTCC 2 cut(s) 1680, 1712
Alw21I GWGCWC 4 cut(s) 695, 1536, 1727, 1825
Alw26I GTCTC 4 cut(s) 370, 1063, 1516, 1797
Alw44I GTGCAC 1 cut(s) 1723
Ama87I CYCGRG 1 cut(s) 2841
Aor13HI TCCGGA 1 cut(s) 1935
AoxI GGCC 6 cut(s) 6, 203, 1062, 1153, 1648, 1975
ApaLI GTGCAC 1 cut(s) 1723
ApeKI GCWGC 5 cut(s) 53, 167, 1481, 1862, 2529
ApoI RAATTY 7 cut(s) 571, 634, 736, 1505, 1525, 2307, 2677
ArsI GACNNNNNNTTYG 4 cut(s) 1583, 1615, 2587, 2619
AspLEI GCGC 5 cut(s) 44, 137, 254, 295, 2886
AspS9I GGNCC 7 cut(s) 162, 184, 279, 1153, 1399, 2066, 2798
AsuC2I CCSGG 5 cut(s) 176, 274, 387, 663, 2870
AsuHPI GGTGA 5 cut(s) 791, 818, 1030, 1779, 2141
AsuII TTCGAA 1 cut(s) 1502
AsuNHI GCTAGC 1 cut(s) 2087
AvaI CYCGRG 1 cut(s) 2841
AvaII GGWCC 6 cut(s) 162, 184, 279, 1399, 2066, 2798
BaeGI GKGCMC 2 cut(s) 1727, 2848
BamHI GGATCC 3 cut(s) 59, 658, 2029
BanI GGYRCC 3 cut(s) 41, 292, 2883
BanII GRGCYC 3 cut(s) 311, 695, 1536
BauI CACGAG 2 cut(s) 917, 2505
BbsI GAAGAC 3 cut(s) 980, 2512, 2556
Bbv12I GWGCWC 4 cut(s) 695, 1536, 1727, 1825
BbvI GCAGC 5 cut(s) 65, 154, 1468, 1849, 2516
BcgI CGANNNNNNTGC 2 cut(s) 908, 942
BciT130I CCWGG 1 cut(s) 159
BciVI GTATCC 1 cut(s) 1612
BclI TGATCA 1 cut(s) 1573
BcnI CCSGG 5 cut(s) 176, 274, 387, 663, 2870
BcoDI GTCTC 4 cut(s) 370, 1063, 1516, 1797
BcuI ACTAGT 2 cut(s) 1316, 2534
BfaI CTAG 8 cut(s) 312, 711, 1221, 1317, 2040, 2088, 2345, 2535
BfmI CTRYAG 2 cut(s) 1482, 1779
BfoI RGCGCY 4 cut(s) 45, 138, 296, 2887
BfrI CTTAAG 1 cut(s) 2462
BfuAI ACCTGC 1 cut(s) 245
BfuI GTATCC 1 cut(s) 1612
BglI GCCNNNNNGGC 2 cut(s) 14, 50
BmcAI AGTACT 1 cut(s) 1114
Bme1390I CCNGG 6 cut(s) 159, 176, 274, 387, 663, 2870
Bme18I GGWCC 6 cut(s) 162, 184, 279, 1399, 2066, 2798
BmeT110I CYCGRG 1 cut(s) 2841
BmgBI CACGTC 1 cut(s) 1433
BmgT120I GGNCC 7 cut(s) 162, 184, 279, 1153, 1399, 2066, 2798
BmiI GGNNCC 9 cut(s) 43, 61, 163, 294, 308, 660, 1301, 2031, 2885
BmrFI CCNGG 6 cut(s) 159, 176, 274, 387, 663, 2870
BmrI ACTGGG 1 cut(s) 1821
BmsI GCATC 8 cut(s) 772, 814, 1123, 1874, 1876, 2326, 2452, 2716
BmtI GCTAGC 1 cut(s) 2091
BmuI ACTGGG 1 cut(s) 1821
BoxI GACNNNNGTC 1 cut(s) 2772
BpiI GAAGAC 3 cut(s) 980, 2512, 2556
BpmI CTGGAG 2 cut(s) 618, 1686
Bpu10I CCTNAGC 1 cut(s) 1843
Bpu14I TTCGAA 1 cut(s) 1502
BpuEI CTTGAG 4 cut(s) 1272, 2256, 2397, 2842
BpuMI CCSGG 5 cut(s) 176, 274, 387, 663, 2870
BsaHI GRCGYC 3 cut(s) 42, 293, 2884
BsaI GGTCTC 1 cut(s) 370
BsaJI CCNNGG 5 cut(s) 34, 158, 960, 1395, 2524
BsaWI WCCGGW 1 cut(s) 1935
BsaXI ACNNNNNCTCC 6 cut(s) 1680, 1710, 1792, 1822, 2502, 2532
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 6 cut(s) 206, 246, 1669, 1827, 2455, 2760
Bse3DI GCAATG 4 cut(s) 1882, 1887, 2073, 2178
BseAI TCCGGA 1 cut(s) 1935
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 5 cut(s) 34, 158, 960, 1395, 2524
BseMI GCAATG 4 cut(s) 1882, 1887, 2073, 2178
BseMII CTCAG 5 cut(s) 312, 389, 1107, 1674, 1857
BseNI ACTGG 6 cut(s) 206, 246, 1669, 1827, 2455, 2760
BseRI GAGGAG 3 cut(s) 517, 1355, 1766
BseSI GKGCMC 2 cut(s) 1727, 2848
BseXI GCAGC 5 cut(s) 65, 154, 1468, 1849, 2516
BsgI GTGCAG 2 cut(s) 456, 2515
Bsh1236I CGCG 4 cut(s) 36, 214, 268, 423
Bsh1285I CGRYCG 1 cut(s) 886
BshFI GGCC 6 cut(s) 8, 205, 1064, 1155, 1650, 1977
BshNI GGYRCC 3 cut(s) 41, 292, 2883
BsiEI CGRYCG 1 cut(s) 886
BsiHKAI GWGCWC 4 cut(s) 695, 1536, 1727, 1825
BsiHKCI CYCGRG 1 cut(s) 2841
BsiSI CCGG 9 cut(s) 45, 138, 175, 202, 274, 386, 662, 1936, 2870
BslFI GGGAC 2 cut(s) 175, 804
BsmAI GTCTC 4 cut(s) 370, 1063, 1516, 1797
BsmFI GGGAC 2 cut(s) 175, 804
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 6 cut(s) 8, 205, 1064, 1155, 1650, 1977
Bso31I GGTCTC 1 cut(s) 370
BsoBI CYCGRG 1 cut(s) 2841
Bsp119I TTCGAA 1 cut(s) 1502
Bsp1286I GDGCHC 6 cut(s) 311, 695, 1536, 1727, 1825, 2848
Bsp13I TCCGGA 1 cut(s) 1935
Bsp1407I TGTACA 1 cut(s) 1764
BspANI GGCC 6 cut(s) 8, 205, 1064, 1155, 1650, 1977
BspCNI CTCAG 5 cut(s) 313, 390, 1108, 1675, 1856
BspEI TCCGGA 1 cut(s) 1935
BspFNI CGCG 4 cut(s) 36, 214, 268, 423
BspHI TCATGA 1 cut(s) 1708
BspLI GGNNCC 9 cut(s) 43, 61, 163, 294, 308, 660, 1301, 2031, 2885
BspMAI CTGCAG 1 cut(s) 1486
BspMI ACCTGC 1 cut(s) 245
BspOI GCTAGC 1 cut(s) 2091
BspQI GCTCTTC 1 cut(s) 2322
BspT104I TTCGAA 1 cut(s) 1502
BspT107I GGYRCC 3 cut(s) 41, 292, 2883
BspTI CTTAAG 1 cut(s) 2462
BspTNI GGTCTC 1 cut(s) 370
BsrBI CCGCTC 2 cut(s) 358, 617
BsrDI GCAATG 4 cut(s) 1882, 1887, 2073, 2178
BsrFI RCCGGY 1 cut(s) 44
BsrGI TGTACA 1 cut(s) 1764
BsrI ACTGG 6 cut(s) 206, 246, 1669, 1827, 2455, 2760
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 5 cut(s) 34, 158, 960, 1395, 2524
BssNI GRCGYC 3 cut(s) 42, 293, 2884
BssSI CACGAG 2 cut(s) 917, 2505
BssT1I CCWWGG 2 cut(s) 1395, 2524
Bst2BI CACGAG 2 cut(s) 917, 2505
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 7 cut(s) 1205, 1256, 1808, 1969, 2645, 2794, 2877
Bst6I CTCTTC 3 cut(s) 494, 1332, 2322
BstACI GRCGYC 3 cut(s) 42, 293, 2884
BstAFI CTTAAG 1 cut(s) 2462
BstAPI GCANNNNNTGC 1 cut(s) 1886
BstAUI TGTACA 1 cut(s) 1764
BstBI TTCGAA 1 cut(s) 1502
BstC8I GCNNGC 8 cut(s) 19, 46, 133, 927, 1873, 1929, 1982, 2089
BstDEI CTNAG 6 cut(s) 321, 398, 1116, 1179, 1683, 1843
BstDSI CCRYGG 1 cut(s) 34
BstFNI CGCG 4 cut(s) 36, 214, 268, 423
BstH2I RGCGCY 4 cut(s) 45, 138, 296, 2887
BstHHI GCGC 5 cut(s) 44, 137, 254, 295, 2886
BstMAI GTCTC 4 cut(s) 370, 1063, 1516, 1797
BstMCI CGRYCG 1 cut(s) 886
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 2 cut(s) 1076, 1597
BstPAI GACNNNNGTC 1 cut(s) 2772
BstSCI CCNGG 6 cut(s) 157, 174, 272, 385, 661, 2868
BstSFI CTRYAG 2 cut(s) 1482, 1779
BstSLI GKGCMC 2 cut(s) 1727, 2848
BstUI CGCG 4 cut(s) 36, 214, 268, 423
BstV1I GCAGC 5 cut(s) 65, 154, 1468, 1849, 2516
BstV2I GAAGAC 3 cut(s) 980, 2512, 2556
BstX2I RGATCY 3 cut(s) 59, 658, 2029
BstYI RGATCY 3 cut(s) 59, 658, 2029
BsuI GTATCC 1 cut(s) 1612
BsuRI GGCC 6 cut(s) 8, 205, 1064, 1155, 1650, 1977
BtgI CCRYGG 1 cut(s) 34
BtgZI GCGATG 1 cut(s) 522
BtrI CACGTC 1 cut(s) 1433
BtsI GCAGTG 2 cut(s) 1476, 2201
BtsIMutI CAGTG 8 cut(s) 1210, 1476, 1834, 1974, 2201, 2331, 2767, 2873
BveI ACCTGC 1 cut(s) 245
Cac8I GCNNGC 8 cut(s) 19, 46, 133, 927, 1873, 1929, 1982, 2089
CciI TCATGA 1 cut(s) 1708
CfoI GCGC 5 cut(s) 44, 137, 254, 295, 2886
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 7 cut(s) 162, 184, 279, 1153, 1399, 2066, 2798
Cfr42I CCGCGG 1 cut(s) 37
Csp6I GTAC 3 cut(s) 1113, 1765, 2409
CspCI CAANNNNNGTGG 2 cut(s) 535, 570
CviQI GTAC 3 cut(s) 1113, 1765, 2409
DdeI CTNAG 6 cut(s) 321, 398, 1116, 1179, 1683, 1843
DinI GGCGCC 3 cut(s) 43, 294, 2885
DraI TTTAAA 1 cut(s) 847
EaeI YGGCCR 3 cut(s) 6, 203, 1975
Eam1104I CTCTTC 3 cut(s) 494, 1332, 2322
EarI CTCTTC 3 cut(s) 494, 1332, 2322
Ecl136II GAGCTC 2 cut(s) 693, 1534
Eco130I CCWWGG 2 cut(s) 1395, 2524
Eco24I GRGCYC 3 cut(s) 311, 695, 1536
Eco31I GGTCTC 1 cut(s) 370
Eco32I GATATC 1 cut(s) 544
Eco47I GGWCC 6 cut(s) 162, 184, 279, 1399, 2066, 2798
Eco53kI GAGCTC 2 cut(s) 693, 1534
Eco57I CTGAAG 2 cut(s) 815, 2247
Eco88I CYCGRG 1 cut(s) 2841
EcoICRI GAGCTC 2 cut(s) 693, 1534
EcoRI GAATTC 2 cut(s) 634, 2677
EcoRII CCWGG 1 cut(s) 157
EcoRV GATATC 1 cut(s) 544
EcoT14I CCWWGG 2 cut(s) 1395, 2524
EcoT38I GRGCYC 3 cut(s) 311, 695, 1536
EgeI GGCGCC 3 cut(s) 43, 294, 2885
EheI GGCGCC 3 cut(s) 43, 294, 2885
ErhI CCWWGG 2 cut(s) 1395, 2524
FalI AAGNNNNNCTT 2 cut(s) 1996, 2028
FaqI GGGAC 2 cut(s) 175, 804
FauI CCCGC 1 cut(s) 76
FbaI TGATCA 1 cut(s) 1573
FblI GTMKAC 1 cut(s) 219
FriOI GRGCYC 3 cut(s) 311, 695, 1536
FspBI CTAG 8 cut(s) 312, 711, 1221, 1317, 2040, 2088, 2345, 2535
GlaI GCGC 5 cut(s) 43, 136, 253, 294, 2885
GsuI CTGGAG 2 cut(s) 618, 1686
HaeII RGCGCY 4 cut(s) 45, 138, 296, 2887
HaeIII GGCC 6 cut(s) 8, 205, 1064, 1155, 1650, 1977
HapII CCGG 9 cut(s) 45, 138, 175, 202, 274, 386, 662, 1936, 2870
HhaI GCGC 5 cut(s) 44, 137, 254, 295, 2886
Hin1I GRCGYC 3 cut(s) 42, 293, 2884
Hin6I GCGC 5 cut(s) 42, 135, 252, 293, 2884
HinP1I GCGC 5 cut(s) 42, 135, 252, 293, 2884
HincII GTYRAC 2 cut(s) 220, 2757
HindII GTYRAC 2 cut(s) 220, 2757
HindIII AAGCTT 3 cut(s) 911, 923, 2274
HpaII CCGG 9 cut(s) 45, 138, 175, 202, 274, 386, 662, 1936, 2870
HphI GGTGA 5 cut(s) 791, 818, 1030, 1779, 2141
Hpy166II GTNNAC 7 cut(s) 220, 327, 1725, 1765, 2066, 2257, 2757
Hpy8I GTNNAC 7 cut(s) 220, 327, 1725, 1765, 2066, 2257, 2757
Hpy99I CGWCG 1 cut(s) 1015
HpyCH4III ACNGT 7 cut(s) 1205, 1256, 1808, 1969, 2645, 2794, 2877
HpyCH4IV ACGT 2 cut(s) 1010, 1432
HpyF3I CTNAG 6 cut(s) 321, 398, 1116, 1179, 1683, 1843
HpySE526I ACGT 2 cut(s) 1010, 1432
Hsp92I GRCGYC 3 cut(s) 42, 293, 2884
HspAI GCGC 5 cut(s) 42, 135, 252, 293, 2884
KasI GGCGCC 3 cut(s) 41, 292, 2883
Kpn2I TCCGGA 1 cut(s) 1935
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
Ksp22I TGATCA 1 cut(s) 1573
KspI CCGCGG 1 cut(s) 37
LguI GCTCTTC 1 cut(s) 2322
LmnI GCTCC 7 cut(s) 306, 355, 504, 515, 698, 1531, 1828
Lsp1109I GCAGC 5 cut(s) 65, 154, 1468, 1849, 2516
LweI GCATC 8 cut(s) 772, 814, 1123, 1874, 1876, 2326, 2452, 2716
MaeI CTAG 8 cut(s) 312, 711, 1221, 1317, 2040, 2088, 2345, 2535
MaeII ACGT 2 cut(s) 1010, 1432
MaeIII GTNAC 3 cut(s) 1124, 1474, 1628
MbiI CCGCTC 2 cut(s) 358, 617
MfeI CAATTG 1 cut(s) 1371
MflI RGATCY 3 cut(s) 59, 658, 2029
MhlI GDGCHC 6 cut(s) 311, 695, 1536, 1727, 1825, 2848
MluI ACGCGT 1 cut(s) 266
Mly113I GGCGCC 3 cut(s) 42, 293, 2884
MlyI GAGTC 5 cut(s) 225, 809, 1352, 1700, 2782
MmeI TCCRAC 2 cut(s) 1790, 2282
MreI CGCCGGCG 1 cut(s) 44
MroI TCCGGA 1 cut(s) 1935
MroNI GCCGGC 1 cut(s) 44
MseI TTAA 8 cut(s) 81, 605, 846, 948, 969, 2265, 2424, 2463
MslI CAYNNNNRTG 1 cut(s) 1077
MspA1I CMGCKG 4 cut(s) 36, 167, 1636, 1871
MspCI CTTAAG 1 cut(s) 2462
MspI CCGG 9 cut(s) 45, 138, 175, 202, 274, 386, 662, 1936, 2870
MspR9I CCNGG 6 cut(s) 159, 176, 274, 387, 663, 2870
MunI CAATTG 1 cut(s) 1371
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 1 cut(s) 159
MvnI CGCG 4 cut(s) 36, 214, 268, 423
NaeI GCCGGC 1 cut(s) 46
NarI GGCGCC 3 cut(s) 42, 293, 2884
NciI CCSGG 5 cut(s) 176, 274, 387, 663, 2870
NgoMIV GCCGGC 1 cut(s) 44
NheI GCTAGC 1 cut(s) 2087
NlaIV GGNNCC 9 cut(s) 43, 61, 163, 294, 308, 660, 1301, 2031, 2885
NmeAIII GCCGAG 1 cut(s) 321
NmuCI GTSAC 2 cut(s) 1124, 1474
NspI RCATGY 2 cut(s) 1076, 1597
NspV TTCGAA 1 cut(s) 1502
PagI TCATGA 1 cut(s) 1708
PciI ACATGT 2 cut(s) 1072, 1593
PciSI GCTCTTC 1 cut(s) 2322
PctI GAATGC 1 cut(s) 592
PdiI GCCGGC 1 cut(s) 46
PfeI GAWTC 7 cut(s) 1082, 1271, 2109, 2417, 2513, 2630, 2837
PfoI TCCNGGA 1 cut(s) 661
PleI GAGTC 5 cut(s) 224, 808, 1351, 1699, 2781
PluTI GGCGCC 3 cut(s) 45, 296, 2887
PpsI GAGTC 5 cut(s) 224, 808, 1351, 1699, 2781
PscI ACATGT 2 cut(s) 1072, 1593
PshAI GACNNNNGTC 1 cut(s) 2772
Psp124BI GAGCTC 2 cut(s) 695, 1536
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 9 cut(s) 43, 61, 163, 294, 308, 660, 1301, 2031, 2885
PspPI GGNCC 7 cut(s) 162, 184, 279, 1153, 1399, 2066, 2798
PstI CTGCAG 1 cut(s) 1486
PsuI RGATCY 3 cut(s) 59, 658, 2029
PvuII CAGCTG 2 cut(s) 1636, 1871
RsaI GTAC 3 cut(s) 1114, 1766, 2410
RsaNI GTAC 3 cut(s) 1113, 1765, 2409
RseI CAYNNNNRTG 1 cut(s) 1077
SacI GAGCTC 2 cut(s) 695, 1536
SacII CCGCGG 1 cut(s) 37
SalI GTCGAC 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 2322
SaqAI TTAA 8 cut(s) 81, 605, 846, 948, 969, 2265, 2424, 2463
Sau96I GGNCC 7 cut(s) 162, 184, 279, 1153, 1399, 2066, 2798
ScaI AGTACT 1 cut(s) 1114
SchI GAGTC 5 cut(s) 225, 809, 1352, 1700, 2782
ScrFI CCNGG 6 cut(s) 159, 176, 274, 387, 663, 2870
SduI GDGCHC 6 cut(s) 311, 695, 1536, 1727, 1825, 2848
SfaNI GCATC 8 cut(s) 772, 814, 1123, 1874, 1876, 2326, 2452, 2716
SfcI CTRYAG 2 cut(s) 1482, 1779
SfoI GGCGCC 3 cut(s) 43, 294, 2885
Sfr303I CCGCGG 1 cut(s) 37
SfuI TTCGAA 1 cut(s) 1502
SgrAI CRCCGGYG 1 cut(s) 44
SgrBI CCGCGG 1 cut(s) 37
SinI GGWCC 6 cut(s) 162, 184, 279, 1399, 2066, 2798
SmiMI CAYNNNNRTG 1 cut(s) 1077
SmlI CTYRAG 5 cut(s) 1287, 2271, 2412, 2462, 2857
SmoI CTYRAG 5 cut(s) 1287, 2271, 2412, 2462, 2857
SpeI ACTAGT 2 cut(s) 1316, 2534
SspDI GGCGCC 3 cut(s) 41, 292, 2883
SspMI CTAG 8 cut(s) 312, 711, 1221, 1317, 2040, 2088, 2345, 2535
SstI GAGCTC 2 cut(s) 695, 1536
StyD4I CCNGG 6 cut(s) 157, 174, 272, 385, 661, 2868
StyI CCWWGG 2 cut(s) 1395, 2524
TaaI ACNGT 7 cut(s) 1205, 1256, 1808, 1969, 2645, 2794, 2877
TaiI ACGT 2 cut(s) 1013, 1435
TaqI TCGA 5 cut(s) 219, 431, 1013, 1502, 2359
TaqII GACCGA 1 cut(s) 172
TatI WGTACW 3 cut(s) 1112, 1764, 2408
TauI GCSGC 7 cut(s) 24, 39, 42, 74, 230, 242, 1980
TfiI GAWTC 7 cut(s) 1082, 1271, 2109, 2417, 2513, 2630, 2837
Tru1I TTAA 8 cut(s) 81, 605, 846, 948, 969, 2265, 2424, 2463
Tru9I TTAA 8 cut(s) 81, 605, 846, 948, 969, 2265, 2424, 2463
TscAI CASTG 8 cut(s) 1210, 1483, 1834, 1974, 2201, 2338, 2767, 2880
TseFI GTSAC 2 cut(s) 1124, 1474
TseI GCWGC 5 cut(s) 53, 167, 1481, 1862, 2529
Tsp45I GTSAC 2 cut(s) 1124, 1474
TspDTI ATGAA 7 cut(s) 879, 1010, 1566, 1666, 1890, 2156, 2309
TspGWI ACGGA 4 cut(s) 1808, 2111, 2412, 2757
TspRI CASTG 8 cut(s) 1210, 1483, 1834, 1974, 2201, 2338, 2767, 2880
Vha464I CTTAAG 1 cut(s) 2462
VneI GTGCAC 1 cut(s) 1723
VpaK11BI GGWCC 6 cut(s) 162, 184, 279, 1399, 2066, 2798
XapI RAATTY 7 cut(s) 571, 634, 736, 1505, 1525, 2307, 2677
XceI RCATGY 2 cut(s) 1076, 1597
XmiI GTMKAC 1 cut(s) 219
XspI CTAG 8 cut(s) 312, 711, 1221, 1317, 2040, 2088, 2345, 2535
ZrmI AGTACT 1 cut(s) 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.