pycom16g19170

snRNA processing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010119
Physical Location & Seq
Forward (+)
16196331 .. 16200769
4439 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3342 bp
ATGAAGAACAAAAGCGAGGGCCTCTACCCTTTTCCTCTCTTCCCGCCCCGAAGGCTGAAACCACGTAGTTTGTTTGATGCTGAAATGGAAAGGAACTCGGCAGCATGTGTGATGCAATGGAGCATCGAGCTAGAGAAAGCCCTGCGCTCCAAGAAACCAGGTCTCTCACTCGGAGCCATTTCGGACGTCGGTGCAAGGCTCCAACAGTTAAGCAGGGAGCCAGATGCTTCTCCTGCTGTCCACCACCTGTTCGACCTAATTCCTGGTGAGGAAAAGCTCTTCTCCAACGCCATCATTCTTCGCCTTGCCAATGCCTTCCATTGTGGAGACAAGAATATAAGACTCTCTATTGTCAGGGCCTTTCTGTTTGAGTACCGGAAGAGGAACAAGCGGAAGGAGTATAATGGAGTTCTGTCCAAAACTCGGATTCATGTGAAGGCAGAACTGCTGAGGATAGTTAAGCTTGTGTTTGACAGTGGGGATGTTGAGGATAGGGCATTGGCTTTGGGTCTGTTTGGTTGTTGGGCACATTTTGCTAGAGAGAGTTCAAGCATACGCTACTTGGTGCTTTCTAGTTTGGTTTCTTCTCACGTTATGGAGGTAAAAGCATCGCTGTTTGCAGCAGGATGTTTTGCCGAGTTATCAGAGGACTTTGCATGTGTTGTCTTGGAGATGCTGCCTCATATGATGACTTCACCAGAAACGTCACCTGCTATAAGGTTGGCTGGAGCACGAGTATTTGCCAAACTTCGGTGCTCACATCCCCTTGTGAATAATGCTTACAAGACATGCCTAAAGCTTCTGTTAGAGTCCGCGGATGAGGGTTGCCAGGTTGCAATGCTGGTTTCTCTCTCCAAACTGTCTTCCAGGTCAACCATTCTTATATCTCAGCAGATGGATTTGCTTCTCTTGTTTCTTAGCCAAGAAAAACCTCTTCAGCTGCGTGCAACAGCAATTATTTCATGTAAGCTAGCATGTCTACCCCTTGATGTATTTGAATCTTCTAAGCTGTTAAGCGTTGCTGAGAATGCAACTTCATCTCCAATCACGTCTGAGGGCCTGTTAGCTATTTCTGTTTTGGTAGATGTGCCAACGAAACTAAAAGGAAGTACAGAGATGGGGTCTCTTGCACCTTGTTCCTTTCTTCTGCCATCTCGTGTGATCTCACTTATCATTGATCGAATCACCTTGATGGTTAAGCCAGTTTTGGATCTTTGTTGGACTGAGTCTCCAGTGTTTCAACAAATTAATTGCCTGCTCAATCTTCTTTTCTGTCTAATTCGAGAATATCCGGATCTGCATGTCGTAGTGCTGGATCAAATATATGTTTTGGTCAAGTCTCTCTCATATGTGCATGACAATCTAGTCATTACATCAGAAGCAGATGCATCTATTCGTGATAATGTAGACTTGAAAGGAGAAAAAAGCAGAACTATCAGATCAAAGCTTTTATTTATTGTCTACAGATTTTTGGTGGCCTTTCTTGAAAATCTCAGTGAAGCTGGTGCCATCTCCACTGAAGTATTTGACAAAGTGAAGCTTCTGGTCGAACTTGTAAGTCAGAGCAACTTGTTTGAATGTTATACATACACACTCTACTCTTTGTTGTTACATTGTCGAATCATTTGGGTTAACTTGGCGCATGGGAGTGAGGGAAGTTACAATCTTGATAAGAACTCGGGTGTATCTCACTTAATGGAGCATGAAATTCGTACAATTGAATGTGCGAAGAGGATGCTGACAGAAAACAAGTGGCCTGCTTACAGAGTCGGGATTTATGCAGCATGTCAGGGAGCCTGGCTCACTGCAACTTTTACATTCGAGCAGTTAGTACCAAAGGTTCATTCTATTTCCTGCTGCTGCTGGATGAGATCATTAGTTCAATTTTCTGATTCTGAGAGGAGACTCAAGTCTCTGCTGTTGACAAAACAAGGTTTAGAGACGAATAAATTTGATCTTATACTTTCTAGTAATGATTCAGGTGAAATCAGCCAAGATTCAGCTAGTAATATCAGGGAGCATATTAACAGTAGGGAGCTCACAGCGGCTTATGATGGTCTTTGTTCTTCTTTGGCAACATTAAAAGGTGATTTCAAAATGGACCAAACTTTTTATTTCCAAAGGTCGTTTTTGTCTTTAAGAGCAAAGGTGCTAGGAGCTGTGGTGGATATAGTTAAAGTTCTTGAAAACATTCAATTTGACCAGCATAGCATTACCAATAATGAACAGGTTGGATACCTCGTATCCTTGAAGAAGTGTACTCAAATATCTGTGCAGTTGAAGAGGCTATCACAAGAATTTGATCTAGTAACAAAATCTTTCATTGACATGGACAGAAAAAGTTCAGGAATCATTTCGGAACTCGCACTATCTTGTTCACTATTGGCCTTTTGTACTGGTTTTTCTCTTTACATTCCAAGCCTAATCGAATCTGTTTCCATTTTCGGTCTGGGAACAACAGAGAACAACTTAGACTCAATGTTGATACAAAATCTAATTGGGCGTTTGGGGGCCAGTAACCATGAAACCAGTGCAACTCTTTGTCTGCTTTTGAATCCAGGTGGCAAGCCCATTGATTGTTTTCACTTGCAGTCCAGAATCCAAGCATGTAAGGTTGGTTCTGTAGCGAAAGATATTCACAGTGTTTGTAGTTCTGTGGTTTTGGGGATTTTTGGCTTGAAAAGTAAGGCAAACGGAGTGCACAATGTGGAAGGTCTCTCCCAAGCAACCAAGGATGGCTTACAACTTCTGTATAGCATCCTAATGAAATGGATGCAAATCCCCTTCCGGACTCCCAAGTACTTCTTTAAATTTAGGCCTTGCCTGGGGTCAGAACTCTTTGCTGTCAGTGAAACGAGAAACCCAGAGGCAATATATGTAGCCTCAGGCTTCAACTTATCATTAAATTTGTGCCTTCAGTTAAGAAATGTGCCACCAGACATCCGAGTCCGGTTGAAAAATTTGTACTGCATAGTCTACAGTGGAGCATCCCTTCAGGAGCCAATAGAAGGTGGAGAAAACAAGGAGCAAAAACAGGCTTGGGAAGCTGATGACATTGTAGAAATGAACGAGAAGCTGTTACAGTATGTAACAGAGTGCAGTACAAAAAAGAGTAATAAGCGTCGAAGGGGTAAAAATGATGGCGAGTTTGTGAGTTCGTTCGCACGTTTTGAACTGAACGAAAGAGGGCAAGGGTTTTCAAGTTGCTTGCTTGATGTTTCTGCTTTTCCAGTGGGTACTTATAGGGTTAAATGGTATAGCTGCTGTATTGATAGTCAGGGTAATTGTTGGAATTTACCCTCTTTGAATGCTGGTCCTGTATTCACTGTACAGAAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1114

Amino Acids

124.24

Weight (kDa)

8.42

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
INTS7_N PF24436 94 - 363 3.3e-29 Integrator complex subunit 7, N-terminal
INTS7_HB PF24437 603 - 778 2.7e-06 Integrator complex subunit 7 helical bundle
INTS7_C_plants PF22966 943 - 1111 1.1e-68 Integrator complex subunit 7, C-terminal domain in plants
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 718
AasI GACNNNNNNGTC 2 cut(s) 1364, 2945
AatII GACGTC 1 cut(s) 189
Acc36I ACCTGC 1 cut(s) 718
AccB1I GGYRCC 1 cut(s) 1505
AccI GTMKAC 4 cut(s) 979, 1407, 1461, 2976
AccII CGCG 1 cut(s) 815
AccIII TCCGGA 2 cut(s) 1291, 2787
AciI CCGC 5 cut(s) 44, 391, 813, 815, 2045
AclWI GGATC 3 cut(s) 1218, 1302, 1323
AcsI RAATTY 7 cut(s) 1707, 1949, 2297, 2810, 2905, 2959, 3292
AcuI CTGAAG 4 cut(s) 920, 1539, 2900, 2978
AcyI GRCGYC 1 cut(s) 186
AdeI CACNNNGTG 1 cut(s) 2707
AfiI CCNNNNNNNGG 4 cut(s) 423, 750, 2825, 3008
AjiI CACGTC 1 cut(s) 1050
AjnI CCWGG 7 cut(s) 157, 262, 828, 866, 1796, 2557, 2823
AjuI GAANNNNNNNTTGG 2 cut(s) 1828, 1860
Alw21I GWGCWC 4 cut(s) 733, 758, 2040, 2703
Alw26I GTCTC 9 cut(s) 167, 321, 1128, 1233, 1344, 1897, 1917, 1934, 2720
Alw44I GTGCAC 1 cut(s) 2699
AlwI GGATC 3 cut(s) 1218, 1302, 1323
Ama87I CYCGRG 1 cut(s) 1678
Aor13HI TCCGGA 2 cut(s) 1291, 2787
AoxI GGCC 8 cut(s) 19, 357, 1057, 1476, 1754, 2385, 2511, 2816
ApaLI GTGCAC 1 cut(s) 2699
ApeKI GCWGC 8 cut(s) 101, 620, 676, 940, 1781, 1857, 1860, 3261
ApoI RAATTY 7 cut(s) 1707, 1949, 2297, 2810, 2905, 2959, 3292
AseI ATTAAT 1 cut(s) 1248
Asp700I GAANNNNTTC 3 cut(s) 2190, 2341, 2353
AspLEI GCGC 2 cut(s) 147, 1642
AspS9I GGNCC 6 cut(s) 19, 357, 1057, 2101, 2511, 3314
AsuHPI GGTGA 6 cut(s) 278, 687, 699, 1177, 1994, 2099
AsuNHI GCTAGC 1 cut(s) 970
AvaI CYCGRG 1 cut(s) 1678
AvaII GGWCC 2 cut(s) 2101, 3314
AxyI CCTNAGG 1 cut(s) 2884
BaeGI GKGCMC 2 cut(s) 529, 2703
BanI GGYRCC 1 cut(s) 1505
BanII GRGCYC 1 cut(s) 2040
BauI CACGAG 2 cut(s) 732, 1155
BbsI GAAGAC 1 cut(s) 855
Bbv12I GWGCWC 4 cut(s) 733, 758, 2040, 2703
BbvCI CCTCAGC 1 cut(s) 449
BbvI GCAGC 8 cut(s) 113, 632, 663, 927, 1793, 1844, 1847, 3248
BccI CCATC 9 cut(s) 299, 889, 1111, 1159, 1186, 1517, 2048, 2729, 3134
BcgI CGANNNNNNTGC 4 cut(s) 1691, 1717, 1725, 1751
BciT130I CCWGG 7 cut(s) 159, 264, 830, 868, 1798, 2559, 2825
BciVI GTATCC 2 cut(s) 2228, 2254
BcoDI GTCTC 9 cut(s) 167, 321, 1128, 1233, 1344, 1897, 1917, 1934, 2720
BfaI CTAG 9 cut(s) 131, 537, 573, 971, 1364, 1968, 2004, 2153, 2306
BfmI CTRYAG 3 cut(s) 1462, 2622, 2977
BfuAI ACCTGC 1 cut(s) 718
BfuI GTATCC 2 cut(s) 2228, 2254
BglI GCCNNNNNGGC 1 cut(s) 52
BisI GCNGC 9 cut(s) 102, 621, 677, 941, 1782, 1858, 1861, 2046, 3262
BlsI GCNGC 9 cut(s) 103, 622, 678, 942, 1783, 1859, 1862, 2047, 3263
BmcAI AGTACT 1 cut(s) 2801
Bme1390I CCNGG 7 cut(s) 159, 264, 830, 868, 1798, 2559, 2825
Bme18I GGWCC 2 cut(s) 2101, 3314
BmeT110I CYCGRG 1 cut(s) 1678
BmgBI CACGTC 1 cut(s) 1050
BmgT120I GGNCC 6 cut(s) 19, 357, 1057, 2101, 2511, 3314
BmiI GGNNCC 7 cut(s) 175, 200, 219, 1507, 1795, 2512, 3000
BmrFI CCNGG 7 cut(s) 159, 264, 830, 868, 1798, 2559, 2825
BmtI GCTAGC 1 cut(s) 974
BoxI GACNNNNGTC 1 cut(s) 1909
BpiI GAAGAC 1 cut(s) 855
BplI GAGNNNNNCTC 2 cut(s) 1785, 1817
BpmI CTGGAG 2 cut(s) 747, 1215
Bpu10I CCTNAGC 1 cut(s) 449
BpuEI CTTGAG 1 cut(s) 1892
BsaAI YACGTR 1 cut(s) 65
BsaBI GATNNNNATC 1 cut(s) 2777
BsaHI GRCGYC 1 cut(s) 186
BsaI GGTCTC 3 cut(s) 167, 1128, 2720
BsaJI CCNNGG 3 cut(s) 813, 2730, 2824
BsaWI WCCGGW 4 cut(s) 375, 1291, 2787, 2949
BsaXI ACNNNNNCTCC 8 cut(s) 720, 750, 1024, 1054, 1213, 1243, 2148, 2178
Bsc4I CCNNNNNNNGG 4 cut(s) 423, 750, 2825, 3008
Bse1I ACTGG 6 cut(s) 1202, 1232, 2401, 2514, 2529, 3230
Bse21I CCTNAGG 1 cut(s) 2884
Bse3DI GCAATG 2 cut(s) 122, 843
Bse8I GATNNNNATC 1 cut(s) 2777
BseAI TCCGGA 2 cut(s) 1291, 2787
BseBI CCWGG 7 cut(s) 159, 264, 830, 868, 1798, 2559, 2825
BseDI CCNNGG 3 cut(s) 813, 2730, 2824
BseJI GATNNNNATC 1 cut(s) 2777
BseLI CCNNNNNNNGG 4 cut(s) 423, 750, 2825, 3008
BseMI GCAATG 2 cut(s) 122, 843
BseMII CTCAG 8 cut(s) 440, 902, 1014, 1044, 1215, 1507, 1887, 2898
BseNI ACTGG 6 cut(s) 1202, 1232, 2401, 2514, 2529, 3230
BseRI GAGGAG 1 cut(s) 1915
BseSI GKGCMC 2 cut(s) 529, 2703
BseXI GCAGC 8 cut(s) 113, 632, 663, 927, 1793, 1844, 1847, 3248
BsgI GTGCAG 2 cut(s) 2294, 3118
Bsh1236I CGCG 1 cut(s) 815
BshFI GGCC 8 cut(s) 21, 359, 1059, 1478, 1756, 2387, 2513, 2818
BshNI GGYRCC 1 cut(s) 1505
BsiHKAI GWGCWC 4 cut(s) 733, 758, 2040, 2703
BsiHKCI CYCGRG 1 cut(s) 1678
BsiSI CCGG 4 cut(s) 376, 1292, 2788, 2950
BslI CCNNNNNNNGG 4 cut(s) 423, 750, 2825, 3008
BsmAI GTCTC 9 cut(s) 167, 321, 1128, 1233, 1344, 1897, 1917, 1934, 2720
BsmBI CGTCTC 1 cut(s) 1934
BsmI GAATGC 2 cut(s) 1033, 3313
BsnI GGCC 8 cut(s) 21, 359, 1059, 1478, 1756, 2387, 2513, 2818
Bso31I GGTCTC 3 cut(s) 167, 1128, 2720
BsoBI CYCGRG 1 cut(s) 1678
Bsp1286I GDGCHC 5 cut(s) 529, 733, 758, 2040, 2703
Bsp13I TCCGGA 2 cut(s) 1291, 2787
Bsp1407I TGTACA 1 cut(s) 3328
Bsp143I GATC 9 cut(s) 1161, 1177, 1210, 1294, 1315, 1439, 1871, 1954, 2302
BspACI CCGC 5 cut(s) 44, 391, 813, 815, 2045
BspANI GGCC 8 cut(s) 21, 359, 1059, 1478, 1756, 2387, 2513, 2818
BspCNI CTCAG 8 cut(s) 441, 901, 1015, 1045, 1216, 1506, 1888, 2897
BspEI TCCGGA 2 cut(s) 1291, 2787
BspFNI CGCG 1 cut(s) 815
BspLI GGNNCC 7 cut(s) 175, 200, 219, 1507, 1795, 2512, 3000
BspMI ACCTGC 1 cut(s) 718
BspOI GCTAGC 1 cut(s) 974
BspPI GGATC 3 cut(s) 1218, 1302, 1323
BspQI GCTCTTC 1 cut(s) 284
BspT107I GGYRCC 1 cut(s) 1505
BspTNI GGTCTC 3 cut(s) 167, 1128, 2720
BsrDI GCAATG 2 cut(s) 122, 843
BsrGI TGTACA 1 cut(s) 3328
BsrI ACTGG 6 cut(s) 1202, 1232, 2401, 2514, 2529, 3230
BssECI CCNNGG 3 cut(s) 813, 2730, 2824
BssMI GATC 9 cut(s) 1161, 1177, 1210, 1294, 1315, 1439, 1871, 1954, 2302
BssNI GRCGYC 1 cut(s) 186
BssSI CACGAG 2 cut(s) 732, 1155
BssT1I CCWWGG 1 cut(s) 2730
Bst2BI CACGAG 2 cut(s) 732, 1155
Bst2UI CCWGG 7 cut(s) 159, 264, 830, 868, 1798, 2559, 2825
Bst4CI ACNGT 8 cut(s) 207, 476, 861, 2030, 2642, 2981, 3084, 3328
Bst6I CTCTTC 6 cut(s) 44, 284, 374, 939, 1724, 2276
BstACI GRCGYC 1 cut(s) 186
BstAPI GCANNNNNTGC 1 cut(s) 533
BstAUI TGTACA 1 cut(s) 3328
BstBAI YACGTR 1 cut(s) 65
BstC8I GCNNGC 6 cut(s) 945, 972, 1256, 1758, 2567, 3209
BstDSI CCRYGG 1 cut(s) 813
BstFNI CGCG 1 cut(s) 815
BstHHI GCGC 2 cut(s) 147, 1642
BstKTI GATC 9 cut(s) 1164, 1180, 1213, 1297, 1318, 1442, 1874, 1957, 2305
BstMAI GTCTC 9 cut(s) 167, 321, 1128, 1233, 1344, 1897, 1917, 1934, 2720
BstMBI GATC 9 cut(s) 1161, 1177, 1210, 1294, 1315, 1439, 1871, 1954, 2302
BstMWI GCNNNNNNNGC 5 cut(s) 52, 233, 533, 1028, 3044
BstNI CCWGG 7 cut(s) 159, 264, 830, 868, 1798, 2559, 2825
BstNSI RCATGY 7 cut(s) 108, 660, 792, 978, 1304, 1788, 2610
BstPAI GACNNNNGTC 1 cut(s) 1909
BstSCI CCNGG 7 cut(s) 157, 262, 828, 866, 1796, 2557, 2823
BstSFI CTRYAG 3 cut(s) 1462, 2622, 2977
BstSLI GKGCMC 2 cut(s) 529, 2703
BstUI CGCG 1 cut(s) 815
BstV1I GCAGC 8 cut(s) 113, 632, 663, 927, 1793, 1844, 1847, 3248
BstV2I GAAGAC 1 cut(s) 855
BstX2I RGATCY 2 cut(s) 1210, 1294
BstYI RGATCY 2 cut(s) 1210, 1294
Bsu36I CCTNAGG 1 cut(s) 2884
BsuI GTATCC 2 cut(s) 2228, 2254
BsuRI GGCC 8 cut(s) 21, 359, 1059, 1478, 1756, 2387, 2513, 2818
BtgI CCRYGG 1 cut(s) 813
BtgZI GCGATG 1 cut(s) 594
BtrI CACGTC 1 cut(s) 1050
BtsI GCAGTG 1 cut(s) 1803
BveI ACCTGC 1 cut(s) 718
Cac8I GCNNGC 6 cut(s) 945, 972, 1256, 1758, 2567, 3209
CfoI GCGC 2 cut(s) 147, 1642
Cfr13I GGNCC 6 cut(s) 19, 357, 1057, 2101, 2511, 3314
Cfr42I CCGCGG 1 cut(s) 816
CseI GACGC 1 cut(s) 3110
CsiI ACCWGGT 1 cut(s) 157
CspCI CAANNNNNGTGG 2 cut(s) 51, 86
DpnI GATC 9 cut(s) 1163, 1179, 1212, 1296, 1317, 1441, 1873, 1956, 2304
DpnII GATC 9 cut(s) 1161, 1177, 1210, 1294, 1315, 1439, 1871, 1954, 2302
DraI TTTAAA 1 cut(s) 2809
DraIII CACNNNGTG 1 cut(s) 2707
DrdI GACNNNNNNGTC 2 cut(s) 1364, 2945
DseDI GACNNNNNNGTC 2 cut(s) 1364, 2945
Eam1104I CTCTTC 6 cut(s) 44, 284, 374, 939, 1724, 2276
EarI CTCTTC 6 cut(s) 44, 284, 374, 939, 1724, 2276
Ecl136II GAGCTC 1 cut(s) 2038
Eco130I CCWWGG 1 cut(s) 2730
Eco147I AGGCCT 1 cut(s) 2818
Eco24I GRGCYC 1 cut(s) 2040
Eco31I GGTCTC 3 cut(s) 167, 1128, 2720
Eco47I GGWCC 2 cut(s) 2101, 3314
Eco53kI GAGCTC 1 cut(s) 2038
Eco57I CTGAAG 4 cut(s) 920, 1539, 2900, 2978
Eco81I CCTNAGG 1 cut(s) 2884
Eco88I CYCGRG 1 cut(s) 1678
EcoICRI GAGCTC 1 cut(s) 2038
EcoO109I RGGNCCY 3 cut(s) 19, 357, 1057
EcoRII CCWGG 7 cut(s) 157, 262, 828, 866, 1796, 2557, 2823
EcoT14I CCWWGG 1 cut(s) 2730
EcoT22I ATGCAT 1 cut(s) 1390
EcoT38I GRGCYC 1 cut(s) 2040
ErhI CCWWGG 1 cut(s) 2730
Esp3I CGTCTC 1 cut(s) 1934
FalI AAGNNNNNCTT 6 cut(s) 1524, 1556, 2723, 2755, 2789, 2821
FauI CCCGC 1 cut(s) 51
FauNDI CATATG 2 cut(s) 684, 1348
FblI GTMKAC 4 cut(s) 979, 1407, 1461, 2976
Fnu4HI GCNGC 9 cut(s) 102, 621, 677, 941, 1782, 1858, 1861, 2046, 3262
FriOI GRGCYC 1 cut(s) 2040
Fsp4HI GCNGC 9 cut(s) 102, 621, 677, 941, 1782, 1858, 1861, 2046, 3262
FspBI CTAG 9 cut(s) 131, 537, 573, 971, 1364, 1968, 2004, 2153, 2306
GlaI GCGC 2 cut(s) 146, 1641
GluI GCNGC 9 cut(s) 102, 621, 677, 941, 1782, 1858, 1861, 2046, 3262
GsuI CTGGAG 2 cut(s) 747, 1215
HaeIII GGCC 8 cut(s) 21, 359, 1059, 1478, 1756, 2387, 2513, 2818
HapII CCGG 4 cut(s) 376, 1292, 2788, 2950
HgaI GACGC 1 cut(s) 3110
HhaI GCGC 2 cut(s) 147, 1642
Hin1I GRCGYC 1 cut(s) 186
Hin6I GCGC 2 cut(s) 145, 1640
HinP1I GCGC 2 cut(s) 145, 1640
HincII GTYRAC 3 cut(s) 873, 1633, 1923
HindII GTYRAC 3 cut(s) 873, 1633, 1923
HindIII AAGCTT 4 cut(s) 461, 797, 1445, 1538
HpaI GTTAAC 1 cut(s) 1633
HpaII CCGG 4 cut(s) 376, 1292, 2788, 2950
HphI GGTGA 6 cut(s) 278, 687, 699, 1177, 1994, 2099
Hpy99I CGWCG 2 cut(s) 191, 3126
HpyCH4III ACNGT 8 cut(s) 207, 476, 861, 2030, 2642, 2981, 3084, 3328
HpyCH4IV ACGT 6 cut(s) 64, 186, 591, 704, 1049, 3166
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 233, 533, 1028, 3044
HpySE526I ACGT 6 cut(s) 64, 186, 591, 704, 1049, 3166
Hsp92I GRCGYC 1 cut(s) 186
HspAI GCGC 2 cut(s) 145, 1640
Kpn2I TCCGGA 2 cut(s) 1291, 2787
KspAI GTTAAC 1 cut(s) 1633
KspI CCGCGG 1 cut(s) 816
Kzo9I GATC 9 cut(s) 1161, 1177, 1210, 1294, 1315, 1439, 1871, 1954, 2302
LguI GCTCTTC 1 cut(s) 284
Lsp1109I GCAGC 8 cut(s) 113, 632, 663, 927, 1793, 1844, 1847, 3248
MabI ACCWGGT 1 cut(s) 157
MaeI CTAG 9 cut(s) 131, 537, 573, 971, 1364, 1968, 2004, 2153, 2306
MaeII ACGT 6 cut(s) 64, 186, 591, 704, 1049, 3166
MaeIII GTNAC 7 cut(s) 705, 1608, 1658, 2308, 2516, 3078, 3088
MalI GATC 9 cut(s) 1163, 1179, 1212, 1296, 1317, 1441, 1873, 1956, 2304
MboI GATC 9 cut(s) 1161, 1177, 1210, 1294, 1315, 1439, 1871, 1954, 2302
MfeI CAATTG 1 cut(s) 1716
MflI RGATCY 2 cut(s) 1210, 1294
MhlI GDGCHC 5 cut(s) 529, 733, 758, 2040, 2703
MlyI GAGTC 8 cut(s) 336, 818, 1235, 1776, 1899, 2468, 2785, 2955
MmeI TCCRAC 5 cut(s) 226, 309, 1199, 2212, 3269
Mph1103I ATGCAT 1 cut(s) 1390
MroI TCCGGA 2 cut(s) 1291, 2787
MroXI GAANNNNTTC 3 cut(s) 2190, 2341, 2353
MslI CAYNNNNRTG 4 cut(s) 1190, 1647, 2327, 2762
MspA1I CMGCKG 3 cut(s) 815, 940, 2045
MspI CCGG 4 cut(s) 376, 1292, 2788, 2950
MspR9I CCNGG 7 cut(s) 159, 264, 830, 868, 1798, 2559, 2825
MunI CAATTG 1 cut(s) 1716
Mva1269I GAATGC 2 cut(s) 1033, 3313
MvaI CCWGG 7 cut(s) 159, 264, 830, 868, 1798, 2559, 2825
MvnI CGCG 1 cut(s) 815
MwoI GCNNNNNNNGC 5 cut(s) 52, 233, 533, 1028, 3044
NdeI CATATG 2 cut(s) 684, 1348
NdeII GATC 9 cut(s) 1161, 1177, 1210, 1294, 1315, 1439, 1871, 1954, 2302
NheI GCTAGC 1 cut(s) 970
NlaIV GGNNCC 7 cut(s) 175, 200, 219, 1507, 1795, 2512, 3000
NmeAIII GCCGAG 2 cut(s) 77, 661
NmuCI GTSAC 1 cut(s) 705
NsiI ATGCAT 1 cut(s) 1390
NspI RCATGY 7 cut(s) 108, 660, 792, 978, 1304, 1788, 2610
PaqCI CACCTGC 1 cut(s) 718
PceI AGGCCT 1 cut(s) 2818
PciSI GCTCTTC 1 cut(s) 284
PctI GAATGC 2 cut(s) 1033, 3313
PdmI GAANNNNTTC 3 cut(s) 2190, 2341, 2353
PflFI GACNNNGTC 1 cut(s) 1225
PkrI GCNGC 9 cut(s) 103, 622, 678, 942, 1783, 1859, 1862, 2047, 3263
PleI GAGTC 8 cut(s) 336, 817, 1234, 1775, 1899, 2468, 2785, 2954
PpsI GAGTC 8 cut(s) 336, 817, 1234, 1775, 1899, 2468, 2785, 2954
Ppu21I YACGTR 1 cut(s) 65
PshAI GACNNNNGTC 1 cut(s) 1909
PshBI ATTAAT 1 cut(s) 1248
Psp124BI GAGCTC 1 cut(s) 2040
Psp6I CCWGG 7 cut(s) 157, 262, 828, 866, 1796, 2557, 2823
PspGI CCWGG 7 cut(s) 157, 262, 828, 866, 1796, 2557, 2823
PspN4I GGNNCC 7 cut(s) 175, 200, 219, 1507, 1795, 2512, 3000
PspPI GGNCC 6 cut(s) 19, 357, 1057, 2101, 2511, 3314
PsrI GAACNNNNNNTAC 4 cut(s) 1824, 1856, 2602, 2634
PsuI RGATCY 2 cut(s) 1210, 1294
PsyI GACNNNGTC 1 cut(s) 1225
PvuII CAGCTG 1 cut(s) 940
RseI CAYNNNNRTG 4 cut(s) 1190, 1647, 2327, 2762
SacI GAGCTC 1 cut(s) 2040
SacII CCGCGG 1 cut(s) 816
SapI GCTCTTC 1 cut(s) 284
SatI GCNGC 9 cut(s) 102, 621, 677, 941, 1782, 1858, 1861, 2046, 3262
Sau3AI GATC 9 cut(s) 1161, 1177, 1210, 1294, 1315, 1439, 1871, 1954, 2302
Sau96I GGNCC 6 cut(s) 19, 357, 1057, 2101, 2511, 3314
ScaI AGTACT 1 cut(s) 2801
SchI GAGTC 8 cut(s) 336, 818, 1235, 1776, 1899, 2468, 2785, 2955
ScrFI CCNGG 7 cut(s) 159, 264, 830, 868, 1798, 2559, 2825
SduI GDGCHC 5 cut(s) 529, 733, 758, 2040, 2703
SexAI ACCWGGT 1 cut(s) 157
SfcI CTRYAG 3 cut(s) 1462, 2622, 2977
Sfr303I CCGCGG 1 cut(s) 816
SgrBI CCGCGG 1 cut(s) 816
SinI GGWCC 2 cut(s) 2101, 3314
SmiMI CAYNNNNRTG 4 cut(s) 1190, 1647, 2327, 2762
SmlI CTYRAG 1 cut(s) 1907
SmoI CTYRAG 1 cut(s) 1907
SseBI AGGCCT 1 cut(s) 2818
SsiI CCGC 5 cut(s) 44, 391, 813, 815, 2045
SspMI CTAG 9 cut(s) 131, 537, 573, 971, 1364, 1968, 2004, 2153, 2306
SstI GAGCTC 1 cut(s) 2040
StuI AGGCCT 1 cut(s) 2818
StyD4I CCNGG 7 cut(s) 157, 262, 828, 866, 1796, 2557, 2823
StyI CCWWGG 1 cut(s) 2730
TaaI ACNGT 8 cut(s) 207, 476, 861, 2030, 2642, 2981, 3084, 3328
TaiI ACGT 6 cut(s) 67, 189, 594, 707, 1052, 3169
TaqI TCGA 9 cut(s) 126, 252, 1180, 1282, 1548, 1618, 1821, 2427, 3124
TaqII GACCGA 1 cut(s) 2435
TatI WGTACW 7 cut(s) 1109, 2258, 2393, 2799, 2964, 3101, 3328
TauI GCSGC 1 cut(s) 2048
TseFI GTSAC 1 cut(s) 705
TseI GCWGC 8 cut(s) 101, 620, 676, 940, 1781, 1857, 1860, 3261
Tsp45I GTSAC 1 cut(s) 705
TspGWI ACGGA 1 cut(s) 2709
Tth111I GACNNNGTC 1 cut(s) 1225
VneI GTGCAC 1 cut(s) 2699
VpaK11BI GGWCC 2 cut(s) 2101, 3314
VspI ATTAAT 1 cut(s) 1248
XapI RAATTY 7 cut(s) 1707, 1949, 2297, 2810, 2905, 2959, 3292
XceI RCATGY 7 cut(s) 108, 660, 792, 978, 1304, 1788, 2610
XcmI CCANNNNNNNNNTGG 1 cut(s) 2446
XmiI GTMKAC 4 cut(s) 979, 1407, 1461, 2976
XmnI GAANNNNTTC 3 cut(s) 2190, 2341, 2353
XspI CTAG 9 cut(s) 131, 537, 573, 971, 1364, 1968, 2004, 2153, 2306
ZraI GACGTC 1 cut(s) 187
ZrmI AGTACT 1 cut(s) 2801
Zsp2I ATGCAT 1 cut(s) 1390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.