pycom16g20960

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010331
Physical Location & Seq
Forward (+)
19098922 .. 19100732
1811 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 483 bp
ATGGGGGAGCCATCACTTTTTGATAAAATGATCAGCCATCTACGTGCAACATCCAAGTATTATACTGGCTATCCAAAGGATCTTGGGCCATCAAGGGTCATCCATTTTACATCAGAACGCGAGTTTGTCCAGCTCCTTCATGAAGGCCATCCCGTGGTTGTTGCATTCACCCTCAGGTGTAATCTCACAAAACATCTTGATGGAGTATTGGAGGAAGCTGCAGCTGAGTTTTATCCACATGTAAAATTTATGCGCGTCGAATGCCCAAAATATCCTGGTTTCTGTATAACACGGCAGAGGAAGGAATATCCATTTATAGAAATATTTCATAGTCCACAACAAGCACAAAACCAGGGAAGGGTTGCTGATGCAAATATTACAAAGTACTCTGTGAAAGTTCTTCCCTTCAATTACGACACCAGTGCCTACGGATTTAGAGAGTTTTTCAAGCGGCATGGGATACAGTCGTCAAGTCCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

18.52

Weight (kDa)

8.96

Isoelectric Point (pI)

56.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 154
AccII CGCG 2 cut(s) 120, 255
AciI CCGC 1 cut(s) 451
AclWI GGATC 1 cut(s) 87
AcsI RAATTY 1 cut(s) 245
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 2 cut(s) 154, 358
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 2 cut(s) 409, 448
AjnI CCWGG 2 cut(s) 274, 351
AluBI AGCT 3 cut(s) 133, 218, 224
AluI AGCT 3 cut(s) 133, 218, 224
AlwI GGATC 1 cut(s) 87
AoxI GGCC 2 cut(s) 86, 145
ApeKI GCWGC 2 cut(s) 218, 221
ApoI RAATTY 1 cut(s) 245
Asp700I GAANNNNTTC 1 cut(s) 324
AspLEI GCGC 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 86
AsuHPI GGTGA 1 cut(s) 160
AxyI CCTNAGG 1 cut(s) 173
BaeI ACNNNNGTAYC 1 cut(s) 452
BbvI GCAGC 2 cut(s) 205, 233
BccI CCATC 5 cut(s) 19, 45, 97, 156, 194
BceAI ACGGC 1 cut(s) 308
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BciT130I CCWGG 2 cut(s) 276, 353
BciVI GTATCC 1 cut(s) 453
BclI TGATCA 1 cut(s) 30
BfmI CTRYAG 1 cut(s) 219
BfuI GTATCC 1 cut(s) 453
BisI GCNGC 3 cut(s) 219, 222, 452
BlsI GCNGC 3 cut(s) 220, 223, 453
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 2 cut(s) 276, 353
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 2 cut(s) 276, 353
BmsI GCATC 1 cut(s) 358
BsaAI YACGTR 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 153, 352
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 358
Bse1I ACTGG 2 cut(s) 70, 420
Bse21I CCTNAGG 1 cut(s) 173
BseBI CCWGG 2 cut(s) 276, 353
BseDI CCNNGG 2 cut(s) 153, 352
BseGI GGATG 3 cut(s) 50, 99, 148
BseLI CCNNNNNNNGG 2 cut(s) 154, 358
BseMII CTCAG 2 cut(s) 187, 216
BseNI ACTGG 2 cut(s) 70, 420
BseXI GCAGC 2 cut(s) 205, 233
Bsh1236I CGCG 2 cut(s) 120, 255
BshFI GGCC 2 cut(s) 88, 147
BslI CCNNNNNNNGG 2 cut(s) 154, 358
BsmI GAATGC 2 cut(s) 164, 266
BsnI GGCC 2 cut(s) 88, 147
Bsp143I GATC 2 cut(s) 30, 79
BspACI CCGC 1 cut(s) 451
BspANI GGCC 2 cut(s) 88, 147
BspCNI CTCAG 2 cut(s) 186, 217
BspFNI CGCG 2 cut(s) 120, 255
BspHI TCATGA 1 cut(s) 139
BspLI GGNNCC 1 cut(s) 9
BspMAI CTGCAG 1 cut(s) 223
BspPI GGATC 1 cut(s) 87
BsrI ACTGG 2 cut(s) 70, 420
BssECI CCNNGG 2 cut(s) 153, 352
BssMI GATC 2 cut(s) 30, 79
Bst2UI CCWGG 2 cut(s) 276, 353
Bst4CI ACNGT 2 cut(s) 465, 480
BstBAI YACGTR 1 cut(s) 44
BstDEI CTNAG 2 cut(s) 173, 225
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 3 cut(s) 50, 99, 148
BstFNI CGCG 2 cut(s) 120, 255
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 2 cut(s) 33, 82
BstMBI GATC 2 cut(s) 30, 79
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstNI CCWGG 2 cut(s) 276, 353
BstNSI RCATGY 1 cut(s) 242
BstSCI CCNGG 2 cut(s) 274, 351
BstSFI CTRYAG 1 cut(s) 219
BstUI CGCG 2 cut(s) 120, 255
BstV1I GCAGC 2 cut(s) 205, 233
BstX2I RGATCY 1 cut(s) 79
BstYI RGATCY 1 cut(s) 79
Bsu36I CCTNAGG 1 cut(s) 173
BsuI GTATCC 1 cut(s) 453
BsuRI GGCC 2 cut(s) 88, 147
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 3 cut(s) 50, 99, 148
BtsIMutI CAGTG 1 cut(s) 427
CciI TCATGA 1 cut(s) 139
CfoI GCGC 1 cut(s) 255
Cfr13I GGNCC 1 cut(s) 86
CseI GACGC 1 cut(s) 244
Csp6I GTAC 1 cut(s) 385
CviAII CATG 3 cut(s) 140, 239, 455
CviJI RGCY 8 cut(s) 10, 36, 69, 88, 133, 147, 218, 224
CviKI_1 RGCY 8 cut(s) 10, 36, 69, 88, 133, 147, 218, 224
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 2 cut(s) 173, 225
DpnI GATC 2 cut(s) 32, 81
DpnII GATC 2 cut(s) 30, 79
Eco81I CCTNAGG 1 cut(s) 173
EcoRII CCWGG 2 cut(s) 274, 351
FaeI CATG 3 cut(s) 143, 242, 458
FaiI YATR 8 cut(s) 63, 141, 240, 251, 287, 317, 330, 456
FatI CATG 3 cut(s) 139, 238, 454
FbaI TGATCA 1 cut(s) 30
Fnu4HI GCNGC 3 cut(s) 219, 222, 452
FokI GGATG 3 cut(s) 37, 86, 135
Fsp4HI GCNGC 3 cut(s) 219, 222, 452
GlaI GCGC 1 cut(s) 254
GluI GCNGC 3 cut(s) 219, 222, 452
HaeIII GGCC 2 cut(s) 88, 147
HgaI GACGC 1 cut(s) 244
HhaI GCGC 1 cut(s) 255
Hin1II CATG 3 cut(s) 143, 242, 458
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 2 cut(s) 335, 476
Hpy188I TCNGA 1 cut(s) 115
Hpy188III TCNNGA 2 cut(s) 140, 197
Hpy8I GTNNAC 2 cut(s) 335, 476
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 5 cut(s) 137, 146, 295, 351, 415
HpyCH4III ACNGT 2 cut(s) 465, 480
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 4 cut(s) 47, 164, 221, 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
HpyF3I CTNAG 2 cut(s) 173, 225
HpySE526I ACGT 1 cut(s) 43
Hsp92II CATG 3 cut(s) 143, 242, 458
HspAI GCGC 1 cut(s) 253
Ksp22I TGATCA 1 cut(s) 30
Kzo9I GATC 2 cut(s) 30, 79
LmnI GCTCC 2 cut(s) 7, 138
LpnPI CCDG 8 cut(s) 51, 143, 160, 261, 288, 338, 365, 433
Lsp1109I GCAGC 2 cut(s) 205, 233
LweI GCATC 1 cut(s) 358
MaeII ACGT 1 cut(s) 43
MalI GATC 2 cut(s) 32, 81
MboI GATC 2 cut(s) 30, 79
MboII GAAGA 1 cut(s) 392
MflI RGATCY 1 cut(s) 79
MluCI AATT 2 cut(s) 245, 409
MnlI CCTC 3 cut(s) 182, 205, 291
MroXI GAANNNNTTC 1 cut(s) 324
MslI CAYNNNNRTG 2 cut(s) 42, 198
MspA1I CMGCKG 1 cut(s) 224
MspR9I CCNGG 2 cut(s) 276, 353
Mva1269I GAATGC 2 cut(s) 164, 266
MvaI CCWGG 2 cut(s) 276, 353
MvnI CGCG 2 cut(s) 120, 255
MwoI GCNNNNNNNGC 1 cut(s) 261
NdeII GATC 2 cut(s) 30, 79
NlaIII CATG 3 cut(s) 143, 242, 458
NlaIV GGNNCC 1 cut(s) 9
NspI RCATGY 1 cut(s) 242
PagI TCATGA 1 cut(s) 139
PciI ACATGT 1 cut(s) 238
PctI GAATGC 2 cut(s) 164, 266
PdmI GAANNNNTTC 1 cut(s) 324
PflMI CCANNNNNTGG 1 cut(s) 154
PkrI GCNGC 3 cut(s) 220, 223, 453
Ppu21I YACGTR 1 cut(s) 44
PscI ACATGT 1 cut(s) 238
Psp6I CCWGG 2 cut(s) 274, 351
PspGI CCWGG 2 cut(s) 274, 351
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 1 cut(s) 86
PstI CTGCAG 1 cut(s) 223
PsuI RGATCY 1 cut(s) 79
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
RseI CAYNNNNRTG 2 cut(s) 42, 198
SatI GCNGC 3 cut(s) 219, 222, 452
Sau3AI GATC 2 cut(s) 30, 79
Sau96I GGNCC 1 cut(s) 86
ScaI AGTACT 1 cut(s) 386
ScrFI CCNGG 2 cut(s) 276, 353
SetI ASST 5 cut(s) 46, 135, 179, 220, 226
SfaNI GCATC 1 cut(s) 358
SfcI CTRYAG 1 cut(s) 219
SmiMI CAYNNNNRTG 2 cut(s) 42, 198
Sse9I AATT 2 cut(s) 245, 409
SsiI CCGC 1 cut(s) 451
SspI AATATT 2 cut(s) 324, 376
StyD4I CCNGG 2 cut(s) 274, 351
TaaI ACNGT 2 cut(s) 465, 480
TaiI ACGT 1 cut(s) 46
TaqI TCGA 1 cut(s) 258
TasI AATT 2 cut(s) 245, 409
TatI WGTACW 1 cut(s) 384
TauI GCSGC 1 cut(s) 454
TscAI CASTG 1 cut(s) 427
TseI GCWGC 2 cut(s) 218, 221
TspDTI ATGAA 3 cut(s) 128, 156, 317
TspGWI ACGGA 1 cut(s) 444
TspRI CASTG 1 cut(s) 427
Van91I CCANNNNNTGG 1 cut(s) 154
XapI RAATTY 1 cut(s) 245
XceI RCATGY 1 cut(s) 242
XmnI GAANNNNTTC 1 cut(s) 324
ZrmI AGTACT 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.