Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0007034

Physical Location & Seq

Genomic Coordinates Forward (+)

19865632 .. 19870954

Gene Structure 5323 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2895 bp

ATGTTATTTTCTGATAGCATGGCAACAGAAAGTCCAATAAGAATGTCAGAAACTAGTGCAAAATGGGCGCCTCGTAAGAAGACTGCTAAACTTGCACTGTCATCTGCTAATATGGCGGCAGAGGAGTTGAAATTGCTTCTCAGGGGCCATAGGTTAAAAAGCAGCAGACAGGATATTTCTCCTAATCGAAGTGGAAGTGCACCCCCAAGTATGGAGGGTTCGTTTTTGGCCATCAATGACCTTCTATCCCAGCAGGACACTAGCATGAATGGAAGCTTGGCAAGTCTAAGTAGTGTTATTGAAAGGTGTGAGACTGAGGAACAATTACGGGCTGATCCAGCTTATTTGGCATATTATTGTGCCAATGTCAATTTGAATCCTAGACTTCCTCGTCCACTTATTTCTTGGGAGAATCGACGTCTGGCACGCCATATTGGTAGTTTCAGCCAGAACTGGGGACCTGAAGATTGCAGTGGCAATTTTCTGTTGCATGTGTCTCAAGGATCACTTCCTACACACAAGGAAGAATCTGAGGATGATCAGTCTTCCCAACACGTTTCTAATGATTGGGTAGATCAGACAAGTGAAGTTTGGTCCGGTGAGGGTGCAGCATCATTTATTGACCACCAACACCAAAATGCGAGTAATTTAATACAGGAAGATTTTGGAGGCTCTGCACCACCTGTACCTAATCACTCTCATACATTAGGCAATGGAATACCTGAGGAATTCATTGATCACAGTAGTGATTCCAGTTCCTTGCATGATCCTGTTATTAATGCGACAACCACCATTAGGCCCAGCGTGGGTGCAGATACTGCCGATGCTACAGAACTTTCACCAAATGATAATTCCTCGCCAACTTCTGTTTCCAGGAGCACGGCTGTTAATGATGCATGTGTTGCGGTCATTGAACCTGAAATGAAGGCTCTTAACATATCGTACTTGATAGAGAATCAAAAGAAACACGAACAATGGGAGCGCAGTTGCCAGAATCATTTCTTGCAGCAGCAGGCTCAGCAACAAAACAATTTTTGTCAAGTTCAAAGTGGCAAGAAGCAAATAACTTCTCAAGGAACTGACAGTACATATATTAGCATGGATCAGCATCTCCATAATACATCCAAGTTTGCGGCGGATGTACAACCAGTTCTTCAGACATCTGGGTTCACACCACCACTATATGCAACTGCAGCAGCCTACGTGAATTCAGCTAATCCATATTACTCAAATATTCAAGGACCAGGCTTTCTCCCTCCACAGTATGTTGGCGGATGTATGAATCCTACTGCTTTTTCTCCATATATTGGTGGATACCATCCTCCGGTTGCTGTCCCTGTGGTTGTTGATGGAACTGTGGGTCCAAGCTTGAATGCTCGAACATCTGGGGTTGCAACTGCGGGCATCCTTTCACCTGGTGCTGATATGCAACATTCAAGCAAGTTCTATGGTCAACTTGGATATCCGCTGCAATCTTCTTTTTCTGAATCTATGTACATGCAATACCATCAACGGCCTTTTGTGGAGTTGTATGGTGTTCCAAGCCAATTTGATCCATCGGCATCAAGAGGTGGTGTTGACTCAAAGAAAGTGTCCAGTCATGGTACTTATTTGGATGAACACAAAAGCCAACATCAGAGAATTGGAAACATGGGGAATTTGAACCCTCAGAGAGGAGGGTCAGTGACTTCTAACTATTTTGGAAGTGCACCAAATGTTGGTATTTTAATGCAGTATCCAACCCCTCCCCTTACTAGTCCAGTTTCGCCAGTACCACCCATAGGTTCAGGCAGAAATACTGGCTTGTATGCATGGCCAGGGCACAGAGGCTTTGAGAGTTATGATGACCCCAAGATTTATAATTTCCTTGAAGAGTTGAAATCTGGCAAAGGCCGCAGATTTGAGCTATCTGATATCACAGGACATATTGTTGAATTCAGTGCTGATCAACATGGGAGTAGATTTATTCAGCAGAAGTTGGAAAACTGTAGTGTTGAAGAGAAGGCTTCTGTATTCAAAGAAGTTCTCCCGCATGCTTCTAAACTAATGACTGATGTTTTCGGTAACTATGTGATTCAGAAGTTTCTTGAGTATGGAACCCCTCAGCAGAGGAAGGAACTTGCCAATCAACTTTTAGGTCAAATTTTGCCCTTAAGTTTGCAGATGTATGGCTGTCGTGTAATCCAAAAGGCACTTGAGGTTATTGAACTTGAACAGAAAGTGCAGCTTGTACATGAGCTGGATGGGCATATTATGAGATGTGTTCGTGATCAAAATGGAAACCATGTCATACAAAAGTGCATTGAGAGCATTCCTACAGAAAAAATTGGGTTTATAATCTCTTCTTTCCGTGGTCAAGTTCCAACACTTTCAATCCATCCTTATGGCTGTCGTGTTATACAGAGAGTTCTGGAGCATTGTACAGATGAGCTGCAATGCCAGTTTATAGTTGACGAGATATTGGAGTCTGTTGCTGGTCTTGCCCAGGACCAGTATGGCAACTATGTCACACAGCATGTATTGGAGAGGGGAAAACCTAATGAAAGAAGGAAAATTATTAACAAGTTATCGGGTCATTTTGTACCGCTGAGCCAGCACAAGTTTGCTTCAAATGTTGTTGAGAAATGTTTGGAGTATGGTGGTGCAGCGGAACGAGAACTGTTAATTAGTGAGATTGTTGGGCATAATGAAGGAAATGATAATTTGTTGGTAATGATGAAGGACCAATATGCAAATTATGTGGTGCAGAAGACTCTCGAAATCTGTACTGATAGTCAACGGGTGTTGTTAATTAATCGTATAAGGGGCCATACAAACACATTGAAGAAGTACACTTACGGGAAGCACATCGTAGCTCGGTTTGAGCAGCTATTTGGTGCAGGTGTGTATTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006417 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009651 GO:0009819 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1903311 GO:1903313 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF630 DUF632 NABP PUF

Protein Analysis

965

Amino Acids

106.59

Weight (kDa)

6.06

Isoelectric Point (pI)

46.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NABP	PF07990	315 - 598	2e-07	Nucleic acid binding protein NABP
PUF	PF00806	642 - 676	6.5e-11	Pumilio-family RNA binding repeat
PUF	PF00806	680 - 711	6.3e-09	Pumilio-family RNA binding repeat
PUF	PF00806	714 - 747	6.1e-09	Pumilio-family RNA binding repeat
PUF	PF00806	786 - 821	4.6e-13	Pumilio-family RNA binding repeat
PUF	PF00806	824 - 856	1.4e-07	Pumilio-family RNA binding repeat
PUF	PF00806	859 - 892	1.7e-09	Pumilio-family RNA binding repeat
PUF	PF00806	904 - 934	3.2e-07	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25880 AT4G25880 AT4G25880 AT4G25880 AT4G25880
fragaria_vesca	FvH4_4g07600 FvH4_4g07600 FvH4_4g07600
malus_domestica	MD13G1245500.v1.1 MD16G1252800.v1.1
prunus_persica	Prupe.1G079300_v2.0.a1 Prupe.1G079300_v2.0.a1
pyrus_communis	pycom13g21640 pycom16g21410
rosa_chinensis	RchiOBHm_Chr4g0400221
rosa_laevigata	RLG00000009203
rosa_multiflora	Rmu_sc0004338.1_g000002
rosa_roxburghii	Rroxscaffold_5G00345180
rosa_rugosa	Rorug04G0023900 Rorug04G0024000
rosa_samantha	Rh4AG098900 Rh4BG097200 Rh4CG108400 Rh4DG093900
rosa_wichuraiana	Rw4G008220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1860, 2336
AarI	CACCTGC	1 cut(s)	2870
AasI	GACNNNNNNGTC	1 cut(s)	390
AatII	GACGTC	1 cut(s)	421
Acc36I	ACCTGC	1 cut(s)	2870
AccB1I	GGYRCC	1 cut(s)	67
AccB7I	CCANNNNNTGG	2 cut(s)	1307, 1718
AclWI	GGATC	5 cut(s)	329, 511, 761, 1110, 1547
AcoI	YGGCCR	2 cut(s)	228, 1814
AcsI	RAATTY	5 cut(s)	728, 1207, 1657, 1934, 2142
AcuI	CTGAAG	2 cut(s)	483, 1139
AcyI	GRCGYC	2 cut(s)	68, 418
AdeI	CACNNNGTG	1 cut(s)	1418
AfiI	CCNNNNNNNGG	9 cut(s)	211, 454, 795, 806, 1307, 1324, 1673, 1718, 1781
AflII	CTTAAG	1 cut(s)	2152
AflIII	ACRYGT	1 cut(s)	553
AhlI	ACTAGT	2 cut(s)	53, 1754
AjnI	CCWGG	5 cut(s)	872, 1243, 1414, 1816, 2484
AjuI	GAANNNNNNNTTGG	6 cut(s)	260, 292, 835, 853, 867, 885
AleI	CACNNNNGTG	1 cut(s)	744
AloI	GAACNNNNNNTCC	2 cut(s)	1345, 1377
Alw21I	GWGCWC	3 cut(s)	202, 881, 1711
Alw26I	GTCTC	2 cut(s)	305, 501
Alw44I	GTGCAC	2 cut(s)	198, 1707
AlwI	GGATC	5 cut(s)	329, 511, 761, 1110, 1547
AlwNI	CAGNNNCTG	1 cut(s)	818
AoxI	GGCC	7 cut(s)	145, 228, 797, 1514, 1814, 1891, 2806
ApaLI	GTGCAC	2 cut(s)	198, 1707
ApoI	RAATTY	5 cut(s)	728, 1207, 1657, 1934, 2142
ArsI	GACNNNNNNTTYG	2 cut(s)	1577, 1609
AseI	ATTAAT	2 cut(s)	777, 2793
Asp700I	GAANNNNTTC	1 cut(s)	998
AspLEI	GCGC	2 cut(s)	70, 984
AspS9I	GGNCC	9 cut(s)	145, 458, 594, 798, 1241, 1361, 2488, 2722, 2806
AsuHPI	GGTGA	3 cut(s)	611, 831, 1404
AvaII	GGWCC	6 cut(s)	458, 594, 1241, 1361, 2488, 2722
AxyI	CCTNAGG	1 cut(s)	723
BaeGI	GKGCMC	3 cut(s)	202, 1711, 1824
BalI	TGGCCA	2 cut(s)	230, 1816
BanI	GGYRCC	1 cut(s)	67
BarI	GAAGNNNNNNTAC	2 cut(s)	2819, 2851
BbsI	GAAGAC	3 cut(s)	86, 537, 2756
Bbv12I	GWGCWC	3 cut(s)	202, 881, 1711
BbvCI	CCTCAGC	1 cut(s)	2103
BccI	CCATC	7 cut(s)	239, 1326, 1343, 1515, 1564, 2237, 2385
BceAI	ACGGC	2 cut(s)	897, 1529
BciT130I	CCWGG	5 cut(s)	874, 1245, 1416, 1818, 2486
BciVI	GTATCC	2 cut(s)	1307, 1746
BclI	TGATCA	4 cut(s)	538, 736, 1945, 2269
BcoDI	GTCTC	2 cut(s)	305, 501
BcuI	ACTAGT	2 cut(s)	53, 1754
BfaI	CTAG	4 cut(s)	54, 261, 381, 1755
BfmI	CTRYAG	4 cut(s)	828, 1191, 1987, 2316
BfoI	RGCGCY	1 cut(s)	71
BfrI	CTTAAG	1 cut(s)	2152
BfuAI	ACCTGC	1 cut(s)	2870
BfuI	GTATCC	2 cut(s)	1307, 1746
BlpI	GCTNAGC	2 cut(s)	1017, 2588
Bme1390I	CCNGG	5 cut(s)	874, 1245, 1416, 1818, 2486
Bme18I	GGWCC	6 cut(s)	458, 594, 1241, 1361, 2488, 2722
BmgT120I	GGNCC	9 cut(s)	145, 458, 594, 798, 1241, 1361, 2488, 2722, 2806
BmiI	GGNNCC	6 cut(s)	69, 146, 459, 1362, 2098, 2807
BmrFI	CCNGG	5 cut(s)	874, 1245, 1416, 1818, 2486
BmrI	ACTGGG	1 cut(s)	463
BmsI	GCATC	6 cut(s)	620, 814, 883, 1117, 1413, 1571
BmuI	ACTGGG	1 cut(s)	463
BpiI	GAAGAC	3 cut(s)	86, 537, 2756
BpmI	CTGGAG	1 cut(s)	2432
Bpu10I	CCTNAGC	1 cut(s)	2103
Bpu1102I	GCTNAGC	2 cut(s)	1017, 2588
BpuEI	CTTGAG	4 cut(s)	483, 1056, 2108, 2216
BsaAI	YACGTR	1 cut(s)	1204
BsaBI	GATNNNNATC	1 cut(s)	1107
BsaHI	GRCGYC	2 cut(s)	68, 418
BsaJI	CCNNGG	3 cut(s)	1817, 2350, 2484
BsaWI	WCCGGW	2 cut(s)	596, 1324
BsaXI	ACNNNNNCTCC	4 cut(s)	206, 236, 1668, 1698
Bsc4I	CCNNNNNNNGG	9 cut(s)	211, 454, 795, 806, 1307, 1324, 1673, 1718, 1781
Bse1I	ACTGG	9 cut(s)	458, 753, 1148, 1596, 1760, 1769, 1804, 2440, 2491
Bse21I	CCTNAGG	1 cut(s)	723
Bse3DI	GCAATG	2 cut(s)	718, 2441
Bse8I	GATNNNNATC	1 cut(s)	1107
BseBI	CCWGG	5 cut(s)	874, 1245, 1416, 1818, 2486
BseDI	CCNNGG	3 cut(s)	1817, 2350, 2484
BseGI	GGATG	9 cut(s)	541, 1121, 1144, 1280, 1318, 1404, 1621, 2248, 2377
BseJI	GATNNNNATC	1 cut(s)	1107
BseLI	CCNNNNNNNGG	9 cut(s)	211, 454, 795, 806, 1307, 1324, 1673, 1718, 1781
BseMI	GCAATG	2 cut(s)	718, 2441
BseMII	CTCAG	8 cut(s)	154, 306, 522, 714, 1031, 1682, 2117, 2579
BseNI	ACTGG	9 cut(s)	458, 753, 1148, 1596, 1760, 1769, 1804, 2440, 2491
BseRI	GAGGAG	2 cut(s)	137, 1689
BseSI	GKGCMC	3 cut(s)	202, 1711, 1824
BseYI	CCCAGC	2 cut(s)	249, 800
BsgI	GTGCAG	6 cut(s)	627, 660, 831, 2243, 2664, 2765
BshFI	GGCC	7 cut(s)	147, 230, 799, 1516, 1816, 1893, 2808
BshNI	GGYRCC	1 cut(s)	67
BsiHKAI	GWGCWC	3 cut(s)	202, 881, 1711
BsiSI	CCGG	2 cut(s)	597, 1325
BslFI	GGGAC	2 cut(s)	471, 1319
BslI	CCNNNNNNNGG	9 cut(s)	211, 454, 795, 806, 1307, 1324, 1673, 1718, 1781
BsmAI	GTCTC	2 cut(s)	305, 501
BsmFI	GGGAC	2 cut(s)	471, 1319
BsmI	GAATGC	2 cut(s)	1378, 2310
BsnI	GGCC	7 cut(s)	147, 230, 799, 1516, 1816, 1893, 2808
Bsp1286I	GDGCHC	4 cut(s)	202, 881, 1711, 1824
Bsp1407I	TGTACA	4 cut(s)	1141, 1494, 2230, 2420
Bsp1720I	GCTNAGC	2 cut(s)	1017, 2588
BspANI	GGCC	7 cut(s)	147, 230, 799, 1516, 1816, 1893, 2808
BspCNI	CTCAG	8 cut(s)	153, 307, 523, 715, 1030, 1681, 2116, 2580
BspLI	GGNNCC	6 cut(s)	69, 146, 459, 1362, 2098, 2807
BspMAI	CTGCAG	1 cut(s)	1195
BspMI	ACCTGC	1 cut(s)	2870
BspPI	GGATC	5 cut(s)	329, 511, 761, 1110, 1547
BspT107I	GGYRCC	1 cut(s)	67
BspTI	CTTAAG	1 cut(s)	2152
BsrDI	GCAATG	2 cut(s)	718, 2441
BsrGI	TGTACA	4 cut(s)	1141, 1494, 2230, 2420
BsrI	ACTGG	9 cut(s)	458, 753, 1148, 1596, 1760, 1769, 1804, 2440, 2491
BssECI	CCNNGG	3 cut(s)	1817, 2350, 2484
BssNI	GRCGYC	2 cut(s)	68, 418
Bst2UI	CCWGG	5 cut(s)	874, 1245, 1416, 1818, 2486
Bst4CI	ACNGT	7 cut(s)	99, 743, 1085, 1263, 1357, 1988, 2661
Bst6I	CTCTTC	3 cut(s)	1866, 1992, 2347
BstACI	GRCGYC	2 cut(s)	68, 418
BstAFI	CTTAAG	1 cut(s)	2152
BstAPI	GCANNNNNTGC	2 cut(s)	818, 902
BstAUI	TGTACA	4 cut(s)	1141, 1494, 2230, 2420
BstBAI	YACGTR	1 cut(s)	1204
BstC8I	GCNNGC	5 cut(s)	427, 1014, 1402, 2034, 2594
BstDEI	CTNAG	9 cut(s)	140, 287, 315, 531, 723, 1017, 1668, 2103, 2588
BstDSI	CCRYGG	1 cut(s)	2350
BstENI	CCTNNNNNAGG	1 cut(s)	1671
BstF5I	GGATG	9 cut(s)	541, 1121, 1144, 1280, 1318, 1404, 1621, 2248, 2377
BstH2I	RGCGCY	1 cut(s)	71
BstHHI	GCGC	2 cut(s)	70, 984
BstMAI	GTCTC	2 cut(s)	305, 501
BstNI	CCWGG	5 cut(s)	874, 1245, 1416, 1818, 2486
BstNSI	RCATGY	5 cut(s)	494, 900, 1501, 2036, 2519
BstSCI	CCNGG	5 cut(s)	872, 1243, 1414, 1816, 2484
BstSFI	CTRYAG	4 cut(s)	828, 1191, 1987, 2316
BstSLI	GKGCMC	3 cut(s)	202, 1711, 1824
BstV2I	GAAGAC	3 cut(s)	86, 537, 2756
BstXI	CCANNNNNNTGG	1 cut(s)	2384
Bsu36I	CCTNAGG	1 cut(s)	723
BsuI	GTATCC	2 cut(s)	1307, 1746
BsuRI	GGCC	7 cut(s)	147, 230, 799, 1516, 1816, 1893, 2808
BtgI	CCRYGG	1 cut(s)	2350
BtsCI	GGATG	9 cut(s)	541, 1121, 1144, 1280, 1318, 1404, 1621, 2248, 2377
BtsI	GCAGTG	1 cut(s)	478
BtsIMutI	CAGTG	4 cut(s)	95, 478, 1689, 1945
BveI	ACCTGC	1 cut(s)	2870
Cac8I	GCNNGC	5 cut(s)	427, 1014, 1402, 2034, 2594
CaiI	CAGNNNCTG	1 cut(s)	818
CfoI	GCGC	2 cut(s)	70, 984
Cfr13I	GGNCC	9 cut(s)	145, 458, 594, 798, 1241, 1361, 2488, 2722, 2806
CsiI	ACCWGGT	1 cut(s)	1414
CspCI	CAANNNNNGTGG	2 cut(s)	2721, 2756
DdeI	CTNAG	9 cut(s)	140, 287, 315, 531, 723, 1017, 1668, 2103, 2588
DinI	GGCGCC	1 cut(s)	69
DraIII	CACNNNGTG	1 cut(s)	1418
DrdI	GACNNNNNNGTC	1 cut(s)	390
DseDI	GACNNNNNNGTC	1 cut(s)	390
EaeI	YGGCCR	2 cut(s)	228, 1814
Eam1104I	CTCTTC	3 cut(s)	1866, 1992, 2347
EarI	CTCTTC	3 cut(s)	1866, 1992, 2347
EciI	GGCGGA	2 cut(s)	1151, 1287
Eco32I	GATATC	2 cut(s)	1463, 1915
Eco47I	GGWCC	6 cut(s)	458, 594, 1241, 1361, 2488, 2722
Eco57I	CTGAAG	2 cut(s)	483, 1139
Eco81I	CCTNAGG	1 cut(s)	723
EcoNI	CCTNNNNNAGG	1 cut(s)	1671
EcoO109I	RGGNCCY	1 cut(s)	458
EcoRI	GAATTC	3 cut(s)	728, 1207, 1934
EcoRII	CCWGG	5 cut(s)	872, 1243, 1414, 1816, 2484
EcoRV	GATATC	2 cut(s)	1463, 1915
EcoT22I	ATGCAT	2 cut(s)	898, 1813
EgeI	GGCGCC	1 cut(s)	69
EheI	GGCGCC	1 cut(s)	69
FalI	AAGNNNNNCTT	4 cut(s)	492, 524, 2211, 2243
FaqI	GGGAC	2 cut(s)	471, 1319
FauI	CCCGC	2 cut(s)	1393, 2037
FbaI	TGATCA	4 cut(s)	538, 736, 1945, 2269
FokI	GGATG	9 cut(s)	548, 1108, 1151, 1287, 1305, 1391, 1628, 2255, 2364
FspBI	CTAG	4 cut(s)	54, 261, 381, 1755
GlaI	GCGC	2 cut(s)	69, 983
GsaI	CCCAGC	2 cut(s)	253, 804
GsuI	CTGGAG	1 cut(s)	2432
HaeII	RGCGCY	1 cut(s)	71
HaeIII	GGCC	7 cut(s)	147, 230, 799, 1516, 1816, 1893, 2808
HapII	CCGG	2 cut(s)	597, 1325
HhaI	GCGC	2 cut(s)	70, 984
Hin1I	GRCGYC	2 cut(s)	68, 418
Hin6I	GCGC	2 cut(s)	68, 982
HinP1I	GCGC	2 cut(s)	68, 982
HincII	GTYRAC	4 cut(s)	1454, 1579, 2452, 2777
HindII	GTYRAC	4 cut(s)	1454, 1579, 2452, 2777
HindIII	AAGCTT	2 cut(s)	274, 1366
HpaII	CCGG	2 cut(s)	597, 1325
HphI	GGTGA	3 cut(s)	611, 831, 1404
Hpy166II	GTNNAC	9 cut(s)	200, 395, 1170, 1454, 1579, 1709, 2452, 2777, 2832
Hpy188III	TCNNGA	5 cut(s)	1566, 2087, 2267, 2411, 2756
Hpy8I	GTNNAC	9 cut(s)	200, 395, 1170, 1454, 1579, 1709, 2452, 2777, 2832
Hpy99I	CGWCG	1 cut(s)	420
HpyAV	CCTTC	7 cut(s)	251, 919, 1996, 2107, 2541, 2684, 2713
HpyCH4III	ACNGT	7 cut(s)	99, 743, 1085, 1263, 1357, 1988, 2661
HpyCH4IV	ACGT	3 cut(s)	418, 555, 1203
HpyF3I	CTNAG	9 cut(s)	140, 287, 315, 531, 723, 1017, 1668, 2103, 2588
HpySE526I	ACGT	3 cut(s)	418, 555, 1203
Hsp92I	GRCGYC	2 cut(s)	68, 418
HspAI	GCGC	2 cut(s)	68, 982
KasI	GGCGCC	1 cut(s)	67
Ksp22I	TGATCA	4 cut(s)	538, 736, 1945, 2269
LmnI	GCTCC	3 cut(s)	876, 979, 2413
LweI	GCATC	6 cut(s)	620, 814, 883, 1117, 1413, 1571
MabI	ACCWGGT	1 cut(s)	1414
MaeI	CTAG	4 cut(s)	54, 261, 381, 1755
MaeII	ACGT	3 cut(s)	418, 555, 1203
MaeIII	GTNAC	3 cut(s)	1684, 2063, 2506
MhlI	GDGCHC	4 cut(s)	202, 881, 1711, 1824
MlsI	TGGCCA	2 cut(s)	230, 1816
MluNI	TGGCCA	2 cut(s)	230, 1816
Mly113I	GGCGCC	1 cut(s)	68
MlyI	GAGTC	3 cut(s)	1574, 2474, 2746
MmeI	TCCRAC	3 cut(s)	1763, 1959, 2387
Mox20I	TGGCCA	2 cut(s)	230, 1816
Mph1103I	ATGCAT	2 cut(s)	898, 1813
MroXI	GAANNNNTTC	1 cut(s)	998
MscI	TGGCCA	2 cut(s)	230, 1816
MslI	CAYNNNNRTG	4 cut(s)	263, 636, 744, 2382
Msp20I	TGGCCA	2 cut(s)	230, 1816
MspA1I	CMGCKG	3 cut(s)	1468, 2587, 2648
MspCI	CTTAAG	1 cut(s)	2152
MspI	CCGG	2 cut(s)	597, 1325
MspR9I	CCNGG	5 cut(s)	874, 1245, 1416, 1818, 2486
Mva1269I	GAATGC	2 cut(s)	1378, 2310
MvaI	CCWGG	5 cut(s)	874, 1245, 1416, 1818, 2486
NarI	GGCGCC	1 cut(s)	68
NlaIV	GGNNCC	6 cut(s)	69, 146, 459, 1362, 2098, 2807
NmuCI	GTSAC	2 cut(s)	1684, 2506
NsiI	ATGCAT	2 cut(s)	898, 1813
NspI	RCATGY	5 cut(s)	494, 900, 1501, 2036, 2519
OliI	CACNNNNGTG	1 cut(s)	744
PacI	TTAATTAA	1 cut(s)	2793
PaeI	GCATGC	1 cut(s)	2036
PaqCI	CACCTGC	1 cut(s)	2870
PctI	GAATGC	2 cut(s)	1378, 2310
PdmI	GAANNNNTTC	1 cut(s)	998
PfeI	GAWTC	9 cut(s)	376, 412, 527, 749, 955, 994, 1282, 1487, 2074
PflMI	CCANNNNNTGG	2 cut(s)	1307, 1718
PfoI	TCCNGGA	1 cut(s)	872
PleI	GAGTC	3 cut(s)	1574, 2473, 2746
PluTI	GGCGCC	1 cut(s)	71
PpsI	GAGTC	3 cut(s)	1574, 2473, 2746
Ppu21I	YACGTR	1 cut(s)	1204
PpuMI	RGGWCCY	1 cut(s)	458
PshBI	ATTAAT	2 cut(s)	777, 2793
PsiI	TTATAA	2 cut(s)	1860, 2336
Psp5II	RGGWCCY	1 cut(s)	458
Psp6I	CCWGG	5 cut(s)	872, 1243, 1414, 1816, 2484
PspFI	CCCAGC	2 cut(s)	249, 800
PspGI	CCWGG	5 cut(s)	872, 1243, 1414, 1816, 2484
PspN4I	GGNNCC	6 cut(s)	69, 146, 459, 1362, 2098, 2807
PspPI	GGNCC	9 cut(s)	145, 458, 594, 798, 1241, 1361, 2488, 2722, 2806
PspPPI	RGGWCCY	1 cut(s)	458
PsrI	GAACNNNNNNTAC	6 cut(s)	202, 234, 1069, 1101, 1134, 1166
PstI	CTGCAG	1 cut(s)	1195
PstNI	CAGNNNCTG	1 cut(s)	818
RseI	CAYNNNNRTG	4 cut(s)	263, 636, 744, 2382
Sau96I	GGNCC	9 cut(s)	145, 458, 594, 798, 1241, 1361, 2488, 2722, 2806
SchI	GAGTC	3 cut(s)	1574, 2474, 2746
ScrFI	CCNGG	5 cut(s)	874, 1245, 1416, 1818, 2486
SduI	GDGCHC	4 cut(s)	202, 881, 1711, 1824
SexAI	ACCWGGT	1 cut(s)	1414
SfaNI	GCATC	6 cut(s)	620, 814, 883, 1117, 1413, 1571
SfcI	CTRYAG	4 cut(s)	828, 1191, 1987, 2316
SfoI	GGCGCC	1 cut(s)	69
SinI	GGWCC	6 cut(s)	458, 594, 1241, 1361, 2488, 2722
SmiMI	CAYNNNNRTG	4 cut(s)	263, 636, 744, 2382
SmlI	CTYRAG	5 cut(s)	498, 1071, 2087, 2152, 2195
SmoI	CTYRAG	5 cut(s)	498, 1071, 2087, 2152, 2195
SpeI	ACTAGT	2 cut(s)	53, 1754
SphI	GCATGC	1 cut(s)	2036
SspDI	GGCGCC	1 cut(s)	67
SspI	AATATT	1 cut(s)	1234
SspMI	CTAG	4 cut(s)	54, 261, 381, 1755
StyD4I	CCNGG	5 cut(s)	872, 1243, 1414, 1816, 2484
TaaI	ACNGT	7 cut(s)	99, 743, 1085, 1263, 1357, 1988, 2661
TaiI	ACGT	3 cut(s)	421, 558, 1206
TaqI	TCGA	4 cut(s)	187, 415, 1378, 2757
TatI	WGTACW	7 cut(s)	1085, 1141, 1494, 2230, 2420, 2765, 2829
TauI	GCSGC	3 cut(s)	119, 1136, 1896
TfiI	GAWTC	9 cut(s)	376, 412, 527, 749, 955, 994, 1282, 1487, 2074
TscAI	CASTG	4 cut(s)	102, 478, 1689, 1945
TseFI	GTSAC	2 cut(s)	1684, 2506
Tsp45I	GTSAC	2 cut(s)	1684, 2506
TspDTI	ATGAA	8 cut(s)	281, 721, 938, 1295, 1632, 2556, 2703, 2732
TspGWI	ACGGA	1 cut(s)	2339
TspRI	CASTG	4 cut(s)	102, 478, 1689, 1945
Van91I	CCANNNNNTGG	2 cut(s)	1307, 1718
Vha464I	CTTAAG	1 cut(s)	2152
VneI	GTGCAC	2 cut(s)	198, 1707
VpaK11BI	GGWCC	6 cut(s)	458, 594, 1241, 1361, 2488, 2722
VspI	ATTAAT	2 cut(s)	777, 2793
XagI	CCTNNNNNAGG	1 cut(s)	1671
XapI	RAATTY	5 cut(s)	728, 1207, 1657, 1934, 2142
XceI	RCATGY	5 cut(s)	494, 900, 1501, 2036, 2519
XcmI	CCANNNNNNNNNTGG	2 cut(s)	402, 2492
XmnI	GAANNNNTTC	1 cut(s)	998
XspI	CTAG	4 cut(s)	54, 261, 381, 1755
ZraI	GACGTC	1 cut(s)	419
Zsp2I	ATGCAT	2 cut(s)	898, 1813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.