pycom16g22180

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009450
Physical Location & Seq
Forward (+)
21765960 .. 21773146
7187 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1656 bp
ATGGCTGCCAGTAAACCGTTGTCATCTTTTGATTCCATGGCGTTGGATAAAGACTGCTCAATTGTCCTTGAGCTATCTGAAAGTGACCCTCTTTTTGATACAAAGAAGAAATTGCTACAAGATAAGGGATTTAATACAAGGCAACAAATCAATGTTAAGGGGTCTTCAGATCCTAATTGGCTCAGTACCACCGTGGAAGTAATGCTTCAGATAGCAACAATCATAAAATCAACTGAGGCAGAGCTTTATTTTGGGCAAGATCAACCAATGGAATCATATGGTCCCAGGAATGAACTTGAGGCTCTCAATTTAATCCTTCAGCTTGTTGAAGCCTCATTCTTTGTTGCTTGTCAAATGCAAAAGAATGTCCTGCAAGAACTGCACAATGTAATTGTTGATATGATTTGTCAGTCTTCTGACAAGCACCGTCCAAAGACTACAATTGTAGAAGGCTACAACTCTGATAAAGAAAAGCAATTGTTACAATGGGGTGAAAACAATGGCATCAAAACAAGGTTGCAGATAGTTTATGTTGAAGGGGCTGGCAGAGGAACCATAGCTAAGGAGGATCTGAAAGTCGGTGATACTGCTCTGGAGATCCCAACATCTTTAATAATCTCCCAGGAGCTTGTGCAAGCTGATATGTATCATGTATTAGAGAAGTTTGAAGGCATTACCCCTGAGACGATGTTGTTGTTGTGGAGCATGAAGGAGAGGCACAATTGTAATTCAAAATACAAGATGTACTTTGACACACTTCCTGAAGAATATCATACTGGGTTGAGTTTTGGAGTCAAGGCGATGATGGCTTTGGGTGGAACCATGCTGTTGGATGAGATAACAGAAGCAAAAGAGCATTTGCATGCTGAATATGATAAGCTCTTCCCTGCACTGTGCAATGAGCATCCTGATATCTTTCCACCGGAGCTTTACAAATGGGAGCAATTCTTGTGGGCATGCGAACTGTGGTACTCAAATAGCATGAAAATCAAGTTTCCTGATGGAAAGCTAAGAACATGCTTGATTCCAGTTGCTGGCTTTCTGAACCATTCACTATACCCACATATAGTGAACTATGGCAGAATAGATGCTTCAGCAAATACCTTGAAATTCTGTCTATCAAGATCATGCAGTGCAGGACAAGAATGCTGTCTTAGTTATGGAGATTTCTCCAGTTCTCATCTAGTTACCTTCTACGGCTTTGTACCACAAGGAGAGAACCCATGCGATGTCATTCTACTAGATTTTGGTTCTGCCCAAGATGAATTTACTGATGGCACCATATCCAACTGGTCCACTCACATGGTGCGGGGTACTTGGTTCTCAAAGAATCGCGATATTTTCTATTATGGCCTGCCCTCCCCATTGTTAGACTTCCTAAGGAGGGCTCATAGTCCCGTGCCGCATACTAAGGCCCTTCTGCCGGAAAATTTGAAGATTGAAATTGAAATTCTTGAAAATCTGCTCTCTATCTGTAATGATATGATGGATGAACTTGGTGATCCCGACCTTGATGACAGAAAAAGCACTAATTGGGATGTAAAGCTGGCAGTGGAGTTCAAGGATATACAGAGAGGTATCGTCTCTTCAGTTTTAACTTCGTGCTACTGTGGCCTCAAACAGGTGAAACACGAATTGTCTGAAATGGACGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

552

Amino Acids

62.46

Weight (kDa)

4.98

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1277
AccB7I CCANNNNNTGG 1 cut(s) 1034
AccII CGCG 1 cut(s) 1335
AciI CCGC 2 cut(s) 1309, 1403
AclWI GGATC 4 cut(s) 164, 576, 592, 1496
AcsI RAATTY 4 cut(s) 1109, 1265, 1429, 1449
AcuI CTGAAG 6 cut(s) 150, 191, 302, 783, 1077, 1572
AdeI CACNNNGTG 1 cut(s) 1306
AfaI GTAC 5 cut(s) 187, 746, 971, 1206, 1315
AfiI CCNNNNNNNGG 4 cut(s) 1034, 1384, 1423, 1621
AjnI CCWGG 2 cut(s) 284, 621
Alw26I GTCTC 2 cut(s) 677, 1588
AlwI GGATC 4 cut(s) 164, 576, 592, 1496
AlwNI CAGNNNCTG 1 cut(s) 1034
AoxI GGCC 3 cut(s) 1351, 1413, 1612
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 4 cut(s) 1109, 1265, 1429, 1449
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
AspS9I GGNCC 3 cut(s) 281, 1293, 1414
AsuHPI GGTGA 4 cut(s) 503, 593, 1511, 1636
AvaII GGWCC 2 cut(s) 281, 1293
AxyI CCTNAGG 1 cut(s) 1379
BanI GGYRCC 1 cut(s) 1277
BanII GRGCYC 1 cut(s) 1390
BarI GAAGNNNNNNTAC 2 cut(s) 1570, 1602
BbsI GAAGAC 2 cut(s) 156, 405
BccI CCATC 4 cut(s) 799, 995, 1268, 1480
BceAI ACGGC 1 cut(s) 1213
BciT130I CCWGG 2 cut(s) 286, 623
BcoDI GTCTC 2 cut(s) 677, 1588
BfaI CTAG 2 cut(s) 1184, 1241
BisI GCNGC 2 cut(s) 6, 1403
BlsI GCNGC 2 cut(s) 7, 1404
Bme1390I CCNGG 2 cut(s) 286, 623
Bme18I GGWCC 2 cut(s) 281, 1293
BmgT120I GGNCC 3 cut(s) 281, 1293, 1414
BmiI GGNNCC 4 cut(s) 283, 553, 820, 1279
BmrFI CCNGG 2 cut(s) 286, 623
BmrI ACTGGG 1 cut(s) 786
BmsI GCATC 3 cut(s) 513, 913, 1078
BmuI ACTGGG 1 cut(s) 786
BpiI GAAGAC 2 cut(s) 156, 405
BpmI CTGGAG 2 cut(s) 614, 1156
Bpu10I CCTNAGC 1 cut(s) 561
BpuEI CTTGAG 2 cut(s) 89, 317
BsaBI GATNNNNATC 1 cut(s) 645
BsaJI CCNNGG 4 cut(s) 36, 192, 284, 621
BsaWI WCCGGW 1 cut(s) 922
Bsc4I CCNNNNNNNGG 4 cut(s) 1034, 1384, 1423, 1621
Bse1I ACTGG 5 cut(s) 9, 781, 1028, 1173, 1295
Bse21I CCTNAGG 1 cut(s) 1379
Bse3DI GCAATG 1 cut(s) 904
Bse8I GATNNNNATC 1 cut(s) 645
BseBI CCWGG 2 cut(s) 286, 623
BseDI CCNNGG 4 cut(s) 36, 192, 284, 621
BseGI GGATG 4 cut(s) 838, 904, 1495, 1543
BseJI GATNNNNATC 1 cut(s) 645
BseLI CCNNNNNNNGG 4 cut(s) 1034, 1384, 1423, 1621
BseMI GCAATG 1 cut(s) 904
BseMII CTCAG 3 cut(s) 196, 225, 672
BseNI ACTGG 5 cut(s) 9, 781, 1028, 1173, 1295
BsgI GTGCAG 3 cut(s) 365, 873, 1155
Bsh1236I CGCG 1 cut(s) 1335
BshFI GGCC 3 cut(s) 1353, 1415, 1614
BshNI GGYRCC 1 cut(s) 1277
BsiSI CCGG 2 cut(s) 923, 1424
BslFI GGGAC 2 cut(s) 267, 1380
BslI CCNNNNNNNGG 4 cut(s) 1034, 1384, 1423, 1621
BsmAI GTCTC 2 cut(s) 677, 1588
BsmBI CGTCTC 2 cut(s) 677, 1588
BsmFI GGGAC 2 cut(s) 267, 1380
BsmI GAATGC 1 cut(s) 1151
BsnI GGCC 3 cut(s) 1353, 1415, 1614
Bsp1286I GDGCHC 1 cut(s) 1390
Bsp143I GATC 6 cut(s) 169, 259, 568, 597, 1124, 1501
Bsp19I CCATGG 1 cut(s) 36
Bsp68I TCGCGA 1 cut(s) 1335
BspACI CCGC 2 cut(s) 1309, 1403
BspANI GGCC 3 cut(s) 1353, 1415, 1614
BspCNI CTCAG 3 cut(s) 195, 226, 673
BspFNI CGCG 1 cut(s) 1335
BspLI GGNNCC 4 cut(s) 283, 553, 820, 1279
BspPI GGATC 4 cut(s) 164, 576, 592, 1496
BspQI GCTCTTC 1 cut(s) 887
BspT107I GGYRCC 1 cut(s) 1277
BsrDI GCAATG 1 cut(s) 904
BsrI ACTGG 5 cut(s) 9, 781, 1028, 1173, 1295
BssECI CCNNGG 4 cut(s) 36, 192, 284, 621
BssMI GATC 6 cut(s) 169, 259, 568, 597, 1124, 1501
BssT1I CCWWGG 1 cut(s) 36
Bst2UI CCWGG 2 cut(s) 286, 623
Bst4CI ACNGT 6 cut(s) 18, 193, 428, 894, 966, 1610
Bst6I CTCTTC 2 cut(s) 887, 1591
BstAPI GCANNNNNTGC 1 cut(s) 379
BstC8I GCNNGC 7 cut(s) 544, 636, 864, 958, 1036, 1355, 1548
BstDEI CTNAG 8 cut(s) 182, 234, 561, 681, 1010, 1154, 1379, 1410
BstDSI CCRYGG 2 cut(s) 36, 192
BstENI CCTNNNNNAGG 1 cut(s) 1619
BstF5I GGATG 4 cut(s) 838, 904, 1495, 1543
BstFNI CGCG 1 cut(s) 1335
BstKTI GATC 6 cut(s) 172, 262, 571, 600, 1127, 1504
BstMAI GTCTC 2 cut(s) 677, 1588
BstMBI GATC 6 cut(s) 169, 259, 568, 597, 1124, 1501
BstMWI GCNNNNNNNGC 3 cut(s) 379, 806, 1611
BstNI CCWGG 2 cut(s) 286, 623
BstNSI RCATGY 3 cut(s) 866, 960, 1020
BstSCI CCNGG 2 cut(s) 284, 621
BstUI CGCG 1 cut(s) 1335
BstV2I GAAGAC 2 cut(s) 156, 405
BstX2I RGATCY 3 cut(s) 169, 568, 597
BstXI CCANNNNNNTGG 3 cut(s) 43, 829, 1303
BstYI RGATCY 3 cut(s) 169, 568, 597
Bsu36I CCTNAGG 1 cut(s) 1379
BsuRI GGCC 3 cut(s) 1353, 1415, 1614
BtgI CCRYGG 2 cut(s) 36, 192
BtgZI GCGATG 2 cut(s) 815, 1242
BtsCI GGATG 4 cut(s) 838, 904, 1495, 1543
BtsI GCAGTG 2 cut(s) 1138, 1557
BtsIMutI CAGTG 3 cut(s) 890, 1138, 1557
BtuMI TCGCGA 1 cut(s) 1335
Cac8I GCNNGC 7 cut(s) 544, 636, 864, 958, 1036, 1355, 1548
CaiI CAGNNNCTG 1 cut(s) 1034
Cfr13I GGNCC 3 cut(s) 281, 1293, 1414
Csp6I GTAC 5 cut(s) 186, 745, 970, 1205, 1314
CspCI CAANNNNNGTGG 2 cut(s) 932, 967
CviQI GTAC 5 cut(s) 186, 745, 970, 1205, 1314
DdeI CTNAG 8 cut(s) 182, 234, 561, 681, 1010, 1154, 1379, 1410
DpnI GATC 6 cut(s) 171, 261, 570, 599, 1126, 1503
DpnII GATC 6 cut(s) 169, 259, 568, 597, 1124, 1501
DraIII CACNNNGTG 1 cut(s) 1306
Eam1104I CTCTTC 2 cut(s) 887, 1591
EarI CTCTTC 2 cut(s) 887, 1591
Eco130I CCWWGG 1 cut(s) 36
Eco24I GRGCYC 1 cut(s) 1390
Eco32I GATATC 1 cut(s) 913
Eco47I GGWCC 2 cut(s) 281, 1293
Eco57I CTGAAG 6 cut(s) 150, 191, 302, 783, 1077, 1572
Eco81I CCTNAGG 1 cut(s) 1379
EcoNI CCTNNNNNAGG 1 cut(s) 1619
EcoO109I RGGNCCY 1 cut(s) 1414
EcoRII CCWGG 2 cut(s) 284, 621
EcoRV GATATC 1 cut(s) 913
EcoT14I CCWWGG 1 cut(s) 36
EcoT38I GRGCYC 1 cut(s) 1390
ErhI CCWWGG 1 cut(s) 36
Esp3I CGTCTC 2 cut(s) 677, 1588
FalI AAGNNNNNCTT 4 cut(s) 189, 221, 731, 763
FaqI GGGAC 2 cut(s) 267, 1380
FauI CCCGC 1 cut(s) 1302
FauNDI CATATG 1 cut(s) 277
Fnu4HI GCNGC 2 cut(s) 6, 1403
FokI GGATG 4 cut(s) 845, 891, 1502, 1550
FriOI GRGCYC 1 cut(s) 1390
Fsp4HI GCNGC 2 cut(s) 6, 1403
FspBI CTAG 2 cut(s) 1184, 1241
GluI GCNGC 2 cut(s) 6, 1403
GsuI CTGGAG 2 cut(s) 614, 1156
HaeIII GGCC 3 cut(s) 1353, 1415, 1614
HapII CCGG 2 cut(s) 923, 1424
HinfI GANTC 5 cut(s) 32, 272, 792, 1024, 1330
HpaII CCGG 2 cut(s) 923, 1424
HphI GGTGA 4 cut(s) 503, 593, 1511, 1636
Hpy166II GTNNAC 3 cut(s) 14, 1072, 1296
Hpy188I TCNGA 8 cut(s) 79, 169, 210, 418, 463, 573, 1044, 1642
Hpy188III TCNNGA 8 cut(s) 593, 761, 908, 998, 1122, 1334, 1454, 1505
Hpy8I GTNNAC 3 cut(s) 14, 1072, 1296
HpyAV CCTTC 7 cut(s) 326, 443, 530, 662, 703, 1201, 1427
HpyCH4III ACNGT 6 cut(s) 18, 193, 428, 894, 966, 1610
HpyF10VI GCNNNNNNNGC 3 cut(s) 379, 806, 1611
HpyF3I CTNAG 8 cut(s) 182, 234, 561, 681, 1010, 1154, 1379, 1410
Kzo9I GATC 6 cut(s) 169, 259, 568, 597, 1124, 1501
LguI GCTCTTC 1 cut(s) 887
LmnI GCTCC 4 cut(s) 625, 702, 925, 940
LweI GCATC 3 cut(s) 513, 913, 1078
MaeI CTAG 2 cut(s) 1184, 1241
MaeIII GTNAC 3 cut(s) 83, 480, 1186
MalI GATC 6 cut(s) 171, 261, 570, 599, 1126, 1503
MboI GATC 6 cut(s) 169, 259, 568, 597, 1124, 1501
MboII GAAGA 7 cut(s) 118, 156, 405, 776, 874, 1447, 1578
MfeI CAATTG 4 cut(s) 60, 441, 476, 721
MflI RGATCY 3 cut(s) 169, 568, 597
MhlI GDGCHC 1 cut(s) 1390
MlyI GAGTC 1 cut(s) 801
MmeI TCCRAC 3 cut(s) 24, 810, 1311
MseI TTAA 5 cut(s) 132, 156, 311, 611, 1595
MslI CAYNNNNRTG 2 cut(s) 861, 1301
MspI CCGG 2 cut(s) 923, 1424
MspR9I CCNGG 2 cut(s) 286, 623
MunI CAATTG 4 cut(s) 60, 441, 476, 721
Mva1269I GAATGC 1 cut(s) 1151
MvaI CCWGG 2 cut(s) 286, 623
MvnI CGCG 1 cut(s) 1335
MwoI GCNNNNNNNGC 3 cut(s) 379, 806, 1611
NcoI CCATGG 1 cut(s) 36
NdeI CATATG 1 cut(s) 277
NdeII GATC 6 cut(s) 169, 259, 568, 597, 1124, 1501
NlaIV GGNNCC 4 cut(s) 283, 553, 820, 1279
NmuCI GTSAC 1 cut(s) 83
NruI TCGCGA 1 cut(s) 1335
NspI RCATGY 3 cut(s) 866, 960, 1020
PaeI GCATGC 2 cut(s) 866, 960
PciSI GCTCTTC 1 cut(s) 887
PctI GAATGC 1 cut(s) 1151
PfeI GAWTC 4 cut(s) 32, 272, 1024, 1330
PflMI CCANNNNNTGG 1 cut(s) 1034
PkrI GCNGC 2 cut(s) 7, 1404
PleI GAGTC 1 cut(s) 800
PpsI GAGTC 1 cut(s) 800
Psp6I CCWGG 2 cut(s) 284, 621
PspGI CCWGG 2 cut(s) 284, 621
PspN4I GGNNCC 4 cut(s) 283, 553, 820, 1279
PspPI GGNCC 3 cut(s) 281, 1293, 1414
PstNI CAGNNNCTG 1 cut(s) 1034
PsuI RGATCY 3 cut(s) 169, 568, 597
RruI TCGCGA 1 cut(s) 1335
RsaI GTAC 5 cut(s) 187, 746, 971, 1206, 1315
RsaNI GTAC 5 cut(s) 186, 745, 970, 1205, 1314
RseI CAYNNNNRTG 2 cut(s) 861, 1301
SapI GCTCTTC 1 cut(s) 887
SaqAI TTAA 5 cut(s) 132, 156, 311, 611, 1595
SatI GCNGC 2 cut(s) 6, 1403
Sau3AI GATC 6 cut(s) 169, 259, 568, 597, 1124, 1501
Sau96I GGNCC 3 cut(s) 281, 1293, 1414
SchI GAGTC 1 cut(s) 801
ScrFI CCNGG 2 cut(s) 286, 623
SduI GDGCHC 1 cut(s) 1390
SfaNI GCATC 3 cut(s) 513, 913, 1078
SinI GGWCC 2 cut(s) 281, 1293
SmiMI CAYNNNNRTG 2 cut(s) 861, 1301
SmlI CTYRAG 2 cut(s) 68, 296
SmoI CTYRAG 2 cut(s) 68, 296
SphI GCATGC 2 cut(s) 866, 960
SsiI CCGC 2 cut(s) 1309, 1403
SspMI CTAG 2 cut(s) 1184, 1241
StyD4I CCNGG 2 cut(s) 284, 621
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 6 cut(s) 18, 193, 428, 894, 966, 1610
TatI WGTACW 1 cut(s) 744
TauI GCSGC 1 cut(s) 1405
TfiI GAWTC 4 cut(s) 32, 272, 1024, 1330
Tru1I TTAA 5 cut(s) 132, 156, 311, 611, 1595
Tru9I TTAA 5 cut(s) 132, 156, 311, 611, 1595
TscAI CASTG 3 cut(s) 897, 1138, 1557
TseFI GTSAC 1 cut(s) 83
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 83
TspDTI ATGAA 5 cut(s) 306, 722, 998, 1278, 1506
TspRI CASTG 3 cut(s) 897, 1138, 1557
Van91I CCANNNNNTGG 1 cut(s) 1034
VpaK11BI GGWCC 2 cut(s) 281, 1293
XagI CCTNNNNNAGG 1 cut(s) 1619
XapI RAATTY 4 cut(s) 1109, 1265, 1429, 1449
XceI RCATGY 3 cut(s) 866, 960, 1020
XspI CTAG 2 cut(s) 1184, 1241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.