Rorug04G0036800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009450
Physical Location & Seq
Forward (+)
5533112 .. 5533318
207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0036800.1

Sequence Viewer

Length: 207 bp
ATGCGGTGTGTAGGTAAGGATTCGTGGCCTGAGCTCATCGGAGCCAAGGCGACAGAGGCGGAGGCAAAAATTGAGAGTGAGAATGATTTAGTCAAGGTTGTCATCGTGAAAGAAGGATCATATGTCACTCATGATTTTCGGTGTAATAGGGTTCGAGTTTGGGTCGATAAACATGGCACCGTCACCAGGGTCCCCACAAGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

68

Amino Acids

7.6

Weight (kDa)

7.81

Isoelectric Point (pI)

11.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
potato_inhibit PF00280 6 - 68 2.9e-26 Potato inhibitor I family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 176
AciI CCGC 2 cut(s) 4, 59
AclWI GGATC 1 cut(s) 124
AfiI CCNNNNNNNGG 2 cut(s) 186, 200
AjnI CCWGG 1 cut(s) 185
AluBI AGCT 1 cut(s) 34
AluI AGCT 1 cut(s) 34
Alw21I GWGCWC 1 cut(s) 36
AlwI GGATC 1 cut(s) 124
AoxI GGCC 1 cut(s) 26
AspS9I GGNCC 1 cut(s) 190
AsuHPI GGTGA 1 cut(s) 175
AvaII GGWCC 1 cut(s) 190
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 36
BciT130I CCWGG 1 cut(s) 187
Bme1390I CCNGG 1 cut(s) 187
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 4 cut(s) 43, 178, 191, 192
BmrFI CCNGG 1 cut(s) 187
Bpu10I CCTNAGC 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 45, 186
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 200
BseBI CCWGG 1 cut(s) 187
BseDI CCNNGG 2 cut(s) 45, 186
BseLI CCNNNNNNNGG 2 cut(s) 186, 200
BseMII CTCAG 1 cut(s) 21
BshFI GGCC 1 cut(s) 28
BshNI GGYRCC 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 36
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 2 cut(s) 186, 200
BsmFI GGGAC 1 cut(s) 176
BsnI GGCC 1 cut(s) 28
Bsp1286I GDGCHC 1 cut(s) 36
Bsp143I GATC 1 cut(s) 116
BspACI CCGC 2 cut(s) 4, 59
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 1 cut(s) 22
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 4 cut(s) 43, 178, 191, 192
BspPI GGATC 1 cut(s) 124
BspT107I GGYRCC 1 cut(s) 176
BssECI CCNNGG 2 cut(s) 45, 186
BssMI GATC 1 cut(s) 116
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 187
Bst4CI ACNGT 1 cut(s) 181
BstDEI CTNAG 1 cut(s) 30
BstKTI GATC 1 cut(s) 119
BstMBI GATC 1 cut(s) 116
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstNI CCWGG 1 cut(s) 187
BstSCI CCNGG 1 cut(s) 185
BsuRI GGCC 1 cut(s) 28
CciI TCATGA 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 190
CviAII CATG 2 cut(s) 131, 173
CviJI RGCY 3 cut(s) 28, 34, 44
CviKI_1 RGCY 3 cut(s) 28, 34, 44
DdeI CTNAG 1 cut(s) 30
DpnI GATC 1 cut(s) 118
DpnII GATC 1 cut(s) 116
EciI GGCGGA 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 190
Eco53kI GAGCTC 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 185
EcoT14I CCWWGG 1 cut(s) 45
EcoT38I GRGCYC 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 2 cut(s) 134, 176
FaiI YATR 4 cut(s) 121, 123, 132, 174
FaqI GGGAC 1 cut(s) 176
FatI CATG 2 cut(s) 130, 172
FauNDI CATATG 1 cut(s) 121
FriOI GRGCYC 1 cut(s) 36
HaeIII GGCC 1 cut(s) 28
Hin1II CATG 2 cut(s) 134, 176
HinfI GANTC 1 cut(s) 20
HphI GGTGA 1 cut(s) 175
Hpy188I TCNGA 1 cut(s) 41
Hpy188III TCNNGA 2 cut(s) 106, 131
HpyAV CCTTC 1 cut(s) 107
HpyCH4III ACNGT 1 cut(s) 181
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
HpyF3I CTNAG 1 cut(s) 30
Hsp92II CATG 2 cut(s) 134, 176
KflI GGGWCCC 1 cut(s) 190
Kzo9I GATC 1 cut(s) 116
LmnI GCTCC 1 cut(s) 41
LpnPI CCDG 3 cut(s) 42, 172, 199
MaeIII GTNAC 2 cut(s) 124, 181
MalI GATC 1 cut(s) 118
MboI GATC 1 cut(s) 116
MhlI GDGCHC 1 cut(s) 36
MluCI AATT 1 cut(s) 69
MnlI CCTC 2 cut(s) 49, 55
MspR9I CCNGG 1 cut(s) 187
MvaI CCWGG 1 cut(s) 187
MwoI GCNNNNNNNGC 1 cut(s) 56
NdeI CATATG 1 cut(s) 121
NdeII GATC 1 cut(s) 116
NlaIII CATG 2 cut(s) 134, 176
NlaIV GGNNCC 4 cut(s) 43, 178, 191, 192
NmuCI GTSAC 2 cut(s) 124, 181
PagI TCATGA 1 cut(s) 130
PfeI GAWTC 1 cut(s) 20
PpuMI RGGWCCY 1 cut(s) 190
Psp124BI GAGCTC 1 cut(s) 36
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 185
PspGI CCWGG 1 cut(s) 185
PspN4I GGNNCC 4 cut(s) 43, 178, 191, 192
PspPI GGNCC 1 cut(s) 190
PspPPI RGGWCCY 1 cut(s) 190
SacI GAGCTC 1 cut(s) 36
Sau3AI GATC 1 cut(s) 116
Sau96I GGNCC 1 cut(s) 190
ScrFI CCNGG 1 cut(s) 187
SduI GDGCHC 1 cut(s) 36
SetI ASST 3 cut(s) 16, 36, 99
SinI GGWCC 1 cut(s) 190
Sse9I AATT 1 cut(s) 69
SsiI CCGC 2 cut(s) 4, 59
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 185
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 1 cut(s) 181
TaqI TCGA 2 cut(s) 154, 165
TasI AATT 1 cut(s) 69
TfiI GAWTC 1 cut(s) 20
TseFI GTSAC 2 cut(s) 124, 181
Tsp45I GTSAC 2 cut(s) 124, 181
VpaK11BI GGWCC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.