pycom16g24690

The H protein shuttles the methylamine group of glycine from the P protein to the T protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012278
Physical Location & Seq
Forward (+)
26280207 .. 26281416
1210 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 453 bp
ATGGCACTGAGATTGTGGGCTTCTTCTACAGCCAATGCACTCAGAATCTCCACTGCCTCCAAATCCCACCTCTCTCCTGCATTTTCTCTCTCCAGATGCTTTTCTACTGTTTTGGATGGATTGAAGTATGCATCTTCACATGAGTGGGTGAAACATGAAGGCTCAGTGGCAACAATTGGTATCACTGATCATGCTCAGGACCATCTTGGAGAAGTAGTGTTTGTTGAGCTGCCAGAATCTGGTCGTTCAGTCTCTCAAGGAGGCAGTTTTGGAGCTGTTGAAAGTGTCAAAGCAACCAGTGATATAAATTCCCCAATCTCCGGCGAAGTCGTCGAGGTTAACTCAAAGCTTACTGAATCTCCTGGTTTGATCAATTCCAGCCCCTATGAAGATGGATGGATGATCGATCAAGCCCAGCAGTCCATCAGAGCTAGAATCCTTGTTGGGACCTAA

Protein Analysis

151

Amino Acids

15.94

Weight (kDa)

5.45

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 41 - 136 5.2e-42 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32470 AT2G35370
fragaria_vesca FvH4_5g21610 FvH4_5g21610
malus_domestica MD06G1031000.v1.1 MD16G1277700.v1.1
prunus_persica Prupe.5G041300_v2.0.a1
pyrus_communis pycom06g02580 pycom16g24690
rosa_chinensis RchiOBHm_Chr7g0209071
rosa_laevigata RLG00000003170
rosa_multiflora Rmu_co8473347.1_g000001
rosa_roxburghii Rroxscaffold_3G00249780
rosa_rugosa Rorug07G0108700
rosa_samantha Rh7AG243200 Rh7BG238200 Rh7CG258400 Rh7DG250200
rosa_wichuraiana Rw7G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 239
AcsI RAATTY 1 cut(s) 307
AfiI CCNNNNNNNGG 2 cut(s) 239, 320
AgsI TTSAA 2 cut(s) 124, 281
AjnI CCWGG 1 cut(s) 361
AleI CACNNNNGTG 1 cut(s) 142
AluBI AGCT 4 cut(s) 229, 275, 349, 431
AluI AGCT 4 cut(s) 229, 275, 349, 431
Alw26I GTCTC 1 cut(s) 256
AlwNI CAGNNNCTG 1 cut(s) 239
ApeKI GCWGC 1 cut(s) 229
ApoI RAATTY 1 cut(s) 307
AspS9I GGNCC 2 cut(s) 199, 447
AsuHPI GGTGA 1 cut(s) 160
AvaII GGWCC 2 cut(s) 199, 447
BbvI GCAGC 1 cut(s) 216
BccI CCATC 5 cut(s) 110, 210, 386, 390, 431
BciT130I CCWGG 1 cut(s) 363
BclI TGATCA 2 cut(s) 187, 369
BcoDI GTCTC 1 cut(s) 256
BfaI CTAG 1 cut(s) 432
BfmI CTRYAG 1 cut(s) 27
BisI GCNGC 1 cut(s) 230
BlsI GCNGC 1 cut(s) 231
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 2 cut(s) 199, 447
BmgT120I GGNCC 2 cut(s) 199, 447
BmiI GGNNCC 1 cut(s) 448
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 2 cut(s) 86, 140
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 1 cut(s) 76
Bpu10I CCTNAGC 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 405
BsaXI ACNNNNNCTCC 4 cut(s) 201, 231, 343, 373
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 320
Bse1I ACTGG 1 cut(s) 297
BseBI CCWGG 1 cut(s) 363
BseCI ATCGAT 1 cut(s) 405
BseGI GGATG 3 cut(s) 121, 401, 405
BseLI CCNNNNNNNGG 2 cut(s) 239, 320
BseMII CTCAG 3 cut(s) 55, 177, 209
BseNI ACTGG 1 cut(s) 297
BseXI GCAGC 1 cut(s) 216
BseYI CCCAGC 1 cut(s) 414
BshVI ATCGAT 1 cut(s) 405
BsiSI CCGG 1 cut(s) 321
BslI CCNNNNNNNGG 2 cut(s) 239, 320
BsmAI GTCTC 1 cut(s) 256
Bsp143I GATC 4 cut(s) 187, 369, 402, 406
BspCNI CTCAG 3 cut(s) 54, 176, 208
BspDI ATCGAT 1 cut(s) 405
BspLI GGNNCC 1 cut(s) 448
BsrI ACTGG 1 cut(s) 297
BssMI GATC 4 cut(s) 187, 369, 402, 406
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 1 cut(s) 109
BstDEI CTNAG 4 cut(s) 8, 41, 163, 195
BstF5I GGATG 3 cut(s) 121, 401, 405
BstKTI GATC 4 cut(s) 190, 372, 405, 409
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 4 cut(s) 187, 369, 402, 406
BstNI CCWGG 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 216
Bsu15I ATCGAT 1 cut(s) 405
BsuTUI ATCGAT 1 cut(s) 405
BtsCI GGATG 3 cut(s) 121, 401, 405
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 5 cut(s) 5, 51, 171, 183, 304
CaiI CAGNNNCTG 1 cut(s) 239
Cfr13I GGNCC 2 cut(s) 199, 447
ClaI ATCGAT 1 cut(s) 405
CviAII CATG 3 cut(s) 140, 155, 191
CviJI RGCY 9 cut(s) 20, 32, 162, 229, 275, 349, 381, 413, 431
CviKI_1 RGCY 9 cut(s) 20, 32, 162, 229, 275, 349, 381, 413, 431
DdeI CTNAG 4 cut(s) 8, 41, 163, 195
DpnI GATC 4 cut(s) 189, 371, 404, 408
DpnII GATC 4 cut(s) 187, 369, 402, 406
Eco47I GGWCC 2 cut(s) 199, 447
EcoO109I RGGNCCY 1 cut(s) 447
EcoRII CCWGG 1 cut(s) 361
EcoT22I ATGCAT 1 cut(s) 133
FaeI CATG 3 cut(s) 143, 158, 194
FaiI YATR 6 cut(s) 129, 141, 156, 192, 305, 387
FatI CATG 3 cut(s) 139, 154, 190
FbaI TGATCA 2 cut(s) 187, 369
Fnu4HI GCNGC 1 cut(s) 230
FokI GGATG 3 cut(s) 128, 408, 412
Fsp4HI GCNGC 1 cut(s) 230
FspBI CTAG 1 cut(s) 432
GluI GCNGC 1 cut(s) 230
GsaI CCCAGC 1 cut(s) 418
GsuI CTGGAG 1 cut(s) 76
HapII CCGG 1 cut(s) 321
Hin1II CATG 3 cut(s) 143, 158, 194
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 4 cut(s) 45, 236, 356, 435
HpaI GTTAAC 1 cut(s) 340
HpaII CCGG 1 cut(s) 321
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 2 cut(s) 44, 428
Hpy188III TCNNGA 2 cut(s) 93, 197
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4V TGCA 3 cut(s) 38, 80, 131
HpyF3I CTNAG 4 cut(s) 8, 41, 163, 195
Hsp92II CATG 3 cut(s) 143, 158, 194
Ksp22I TGATCA 2 cut(s) 187, 369
KspAI GTTAAC 1 cut(s) 340
Kzo9I GATC 4 cut(s) 187, 369, 402, 406
LmnI GCTCC 1 cut(s) 272
Lsp1109I GCAGC 1 cut(s) 216
LweI GCATC 2 cut(s) 86, 140
MaeI CTAG 1 cut(s) 432
MalI GATC 4 cut(s) 189, 371, 404, 408
MboI GATC 4 cut(s) 187, 369, 402, 406
MboII GAAGA 3 cut(s) 15, 126, 401
MfeI CAATTG 1 cut(s) 174
MluCI AATT 3 cut(s) 174, 307, 373
MnlI CCTC 4 cut(s) 67, 80, 254, 328
Mph1103I ATGCAT 1 cut(s) 133
MseI TTAA 1 cut(s) 339
MslI CAYNNNNRTG 1 cut(s) 142
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 1 cut(s) 363
MunI CAATTG 1 cut(s) 174
MvaI CCWGG 1 cut(s) 363
NdeII GATC 4 cut(s) 187, 369, 402, 406
NlaIII CATG 3 cut(s) 143, 158, 194
NlaIV GGNNCC 1 cut(s) 448
NsiI ATGCAT 1 cut(s) 133
OliI CACNNNNGTG 1 cut(s) 142
PfeI GAWTC 4 cut(s) 45, 236, 356, 435
PflMI CCANNNNNTGG 1 cut(s) 239
PkrI GCNGC 1 cut(s) 231
PpuMI RGGWCCY 1 cut(s) 447
Psp5II RGGWCCY 1 cut(s) 447
Psp6I CCWGG 1 cut(s) 361
PspFI CCCAGC 1 cut(s) 414
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 1 cut(s) 448
PspPI GGNCC 2 cut(s) 199, 447
PspPPI RGGWCCY 1 cut(s) 447
PstNI CAGNNNCTG 1 cut(s) 239
RseI CAYNNNNRTG 1 cut(s) 142
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 1 cut(s) 230
Sau3AI GATC 4 cut(s) 187, 369, 402, 406
Sau96I GGNCC 2 cut(s) 199, 447
ScrFI CCNGG 1 cut(s) 363
SetI ASST 7 cut(s) 72, 231, 277, 339, 351, 433, 452
SfaNI GCATC 2 cut(s) 86, 140
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 2 cut(s) 199, 447
SmiMI CAYNNNNRTG 1 cut(s) 142
SmlI CTYRAG 1 cut(s) 255
SmoI CTYRAG 1 cut(s) 255
Sse9I AATT 3 cut(s) 174, 307, 373
SspMI CTAG 1 cut(s) 432
StyD4I CCNGG 1 cut(s) 361
TaaI ACNGT 1 cut(s) 109
TaqI TCGA 2 cut(s) 333, 405
TasI AATT 3 cut(s) 174, 307, 373
TfiI GAWTC 4 cut(s) 45, 236, 356, 435
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 5 cut(s) 12, 58, 171, 190, 304
TseI GCWGC 1 cut(s) 229
TspDTI ATGAA 2 cut(s) 171, 402
TspRI CASTG 5 cut(s) 12, 58, 171, 190, 304
Van91I CCANNNNNTGG 1 cut(s) 239
VpaK11BI GGWCC 2 cut(s) 199, 447
XapI RAATTY 1 cut(s) 307
XspI CTAG 1 cut(s) 432
Zsp2I ATGCAT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.