pycom17g00420

Translocon-associated protein subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009086
Physical Location & Seq
Reverse (-)
298138 .. 300604
2467 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
ATGTACCACTTATATTTCTGGGTTTTCCTTTGGTCTGTAAGTGTGAATTTAACATTTTTCCGAGTTGAGGATCCCAAGCAAACGGGTCCAACCAAACCTCACCTCCCCTCACCTCAGCGACTCCGTCACGACTCACCACCTCCATCCGAAATCCAAATGGCGATTCCGATCGGCAGCGCTCTCCTCTCGACGGTGGTAGCTCTCTTGCTATTCACGTCGGCGCTCGCGAGCTCAGAGTTGCCCTTCATCATCGCCCACAAGAAGGCCTCCCTCAACCGGCTCAAGTCCGGCGTCGAGCGCGTCCTTGTCTCCATCGACATCTACAACCAAGGATCGTTCTTAACGGCTTATGATGTAAGCTTGAGTGATGATAGCTGGCCTCAAGATATCTTTGAGGTTGTCAGCGGTAACACTTCCAAGTCATGGGAAAGGCTTGATGCAGGTGGCATTGTATCCCACTCCTTTGAATTGGAGGCCAAAGAAAAATTACTTTTCAACGGTGCACCAGCTCTTATTACATTCCGCATTCCCACAAAGGCTGCTTTACAGGAGTCTTATTCAACTCCAATATTTCCTTTGGATGTTCTGGCAGACAGGCCTCCTGAGAAGAAGTTTGAATGGGTTAAGAGGTTTCTGGCAAAGTACGGGTCTCTGATCTCTGTTGTCTCCATTGTGGTTCTATTTGTGTACCTGGTTGCCAGTCCATCCAAATCCGGTGCTGCAAAAGGGAGCAAGAAGAAGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
TRAP_beta

Protein Analysis

249

Amino Acids

27.54

Weight (kDa)

9.4

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAP_beta PF05753 68 - 244 5.1e-52 Translocon-associated protein beta (TRAPB)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 431
Acc36I ACCTGC 1 cut(s) 431
AccB7I CCANNNNNTGG 1 cut(s) 423
AccII CGCG 2 cut(s) 227, 300
AciI CCGC 2 cut(s) 405, 523
AclWI GGATC 3 cut(s) 65, 78, 340
AcsI RAATTY 1 cut(s) 46
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 3 cut(s) 5, 644, 689
AfeI AGCGCT 1 cut(s) 178
AfiI CCNNNNNNNGG 5 cut(s) 67, 190, 262, 276, 423
AgsI TTSAA 4 cut(s) 467, 496, 561, 617
AjiI CACGTC 1 cut(s) 216
AjnI CCWGG 1 cut(s) 690
AjuI GAANNNNNNNTTGG 2 cut(s) 147, 179
AluBI AGCT 5 cut(s) 200, 231, 360, 375, 509
AluI AGCT 5 cut(s) 200, 231, 360, 375, 509
Alw21I GWGCWC 2 cut(s) 233, 505
Alw26I GTCTC 3 cut(s) 313, 654, 670
Alw44I GTGCAC 1 cut(s) 501
AlwI GGATC 3 cut(s) 65, 78, 340
Aor51HI AGCGCT 1 cut(s) 178
AoxI GGCC 4 cut(s) 264, 377, 474, 596
ApaLI GTGCAC 1 cut(s) 501
ApeKI GCWGC 3 cut(s) 174, 539, 719
ApoI RAATTY 1 cut(s) 46
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AspLEI GCGC 3 cut(s) 179, 223, 300
AspS9I GGNCC 1 cut(s) 86
AsuHPI GGTGA 3 cut(s) 92, 102, 126
AvaII GGWCC 1 cut(s) 86
BaeGI GKGCMC 1 cut(s) 505
BamHI GGATCC 1 cut(s) 70
BanII GRGCYC 1 cut(s) 233
Bbv12I GWGCWC 2 cut(s) 233, 505
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 3 cut(s) 186, 526, 706
BccI CCATC 3 cut(s) 151, 320, 712
BceAI ACGGC 1 cut(s) 360
BciT130I CCWGG 1 cut(s) 692
BciVI GTATCC 1 cut(s) 463
BcoDI GTCTC 3 cut(s) 313, 654, 670
BfoI RGCGCY 2 cut(s) 180, 224
BfuAI ACCTGC 1 cut(s) 431
BfuI GTATCC 1 cut(s) 463
BisI GCNGC 3 cut(s) 175, 540, 720
BlsI GCNGC 3 cut(s) 176, 541, 721
Bme1390I CCNGG 1 cut(s) 692
Bme18I GGWCC 1 cut(s) 86
BmgBI CACGTC 1 cut(s) 216
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 2 cut(s) 72, 87
BmrFI CCNGG 1 cut(s) 692
BmsI GCATC 1 cut(s) 427
Bpu10I CCTNAGC 1 cut(s) 114
BpuEI CTTGAG 3 cut(s) 266, 366, 382
BsaBI GATNNNNATC 1 cut(s) 167
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 654
BsaJI CCNNGG 1 cut(s) 328
BsaWI WCCGGW 1 cut(s) 713
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 5 cut(s) 67, 190, 262, 276, 423
Bse118I RCCGGY 1 cut(s) 276
Bse1I ACTGG 1 cut(s) 699
Bse8I GATNNNNATC 1 cut(s) 167
BseBI CCWGG 1 cut(s) 692
BseDI CCNNGG 1 cut(s) 328
BseGI GGATG 3 cut(s) 143, 586, 704
BseJI GATNNNNATC 1 cut(s) 167
BseLI CCNNNNNNNGG 5 cut(s) 67, 190, 262, 276, 423
BseMII CTCAG 3 cut(s) 128, 246, 594
BseNI ACTGG 1 cut(s) 699
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 1 cut(s) 505
BseXI GCAGC 3 cut(s) 186, 526, 706
Bsh1236I CGCG 2 cut(s) 227, 300
Bsh1285I CGRYCG 1 cut(s) 171
BshFI GGCC 4 cut(s) 266, 379, 476, 598
BsiEI CGRYCG 1 cut(s) 171
BsiHKAI GWGCWC 2 cut(s) 233, 505
BsiSI CCGG 3 cut(s) 277, 288, 714
BslI CCNNNNNNNGG 5 cut(s) 67, 190, 262, 276, 423
BsmAI GTCTC 3 cut(s) 313, 654, 670
BsmI GAATGC 1 cut(s) 525
BsnI GGCC 4 cut(s) 266, 379, 476, 598
Bso31I GGTCTC 1 cut(s) 654
Bsp1286I GDGCHC 2 cut(s) 233, 505
Bsp143I GATC 4 cut(s) 70, 168, 332, 654
Bsp68I TCGCGA 1 cut(s) 227
BspACI CCGC 2 cut(s) 405, 523
BspANI GGCC 4 cut(s) 266, 379, 476, 598
BspCNI CTCAG 3 cut(s) 127, 245, 595
BspFNI CGCG 2 cut(s) 227, 300
BspLI GGNNCC 2 cut(s) 72, 87
BspMI ACCTGC 1 cut(s) 431
BspPI GGATC 3 cut(s) 65, 78, 340
BspTNI GGTCTC 1 cut(s) 654
BsrFI RCCGGY 1 cut(s) 276
BsrI ACTGG 1 cut(s) 699
BssAI RCCGGY 1 cut(s) 276
BssECI CCNNGG 1 cut(s) 328
BssMI GATC 4 cut(s) 70, 168, 332, 654
BssNI GRCGYC 1 cut(s) 291
BssT1I CCWWGG 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 692
Bst4CI ACNGT 2 cut(s) 193, 500
BstACI GRCGYC 1 cut(s) 291
BstC8I GCNNGC 3 cut(s) 225, 229, 377
BstDEI CTNAG 3 cut(s) 114, 232, 603
BstF5I GGATG 3 cut(s) 143, 586, 704
BstFNI CGCG 2 cut(s) 227, 300
BstH2I RGCGCY 2 cut(s) 180, 224
BstHHI GCGC 3 cut(s) 179, 223, 300
BstKTI GATC 4 cut(s) 73, 171, 335, 657
BstMAI GTCTC 3 cut(s) 313, 654, 670
BstMBI GATC 4 cut(s) 70, 168, 332, 654
BstMCI CGRYCG 1 cut(s) 171
BstMWI GCNNNNNNNGC 1 cut(s) 297
BstNI CCWGG 1 cut(s) 692
BstSCI CCNGG 1 cut(s) 690
BstSLI GKGCMC 1 cut(s) 505
BstUI CGCG 2 cut(s) 227, 300
BstV1I GCAGC 3 cut(s) 186, 526, 706
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 4 cut(s) 266, 379, 476, 598
BtgZI GCGATG 1 cut(s) 235
BtrI CACGTC 1 cut(s) 216
BtsCI GGATG 3 cut(s) 143, 586, 704
BtuMI TCGCGA 1 cut(s) 227
BveI ACCTGC 1 cut(s) 431
Cac8I GCNNGC 3 cut(s) 225, 229, 377
CfoI GCGC 3 cut(s) 179, 223, 300
Cfr10I RCCGGY 1 cut(s) 276
Cfr13I GGNCC 1 cut(s) 86
CseI GACGC 2 cut(s) 280, 289
CsiI ACCWGGT 1 cut(s) 690
Csp6I GTAC 3 cut(s) 4, 643, 688
CviAII CATG 1 cut(s) 423
CviQI GTAC 3 cut(s) 4, 643, 688
DdeI CTNAG 3 cut(s) 114, 232, 603
DpnI GATC 4 cut(s) 72, 170, 334, 656
DpnII GATC 4 cut(s) 70, 168, 332, 654
Ecl136II GAGCTC 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 328
Eco147I AGGCCT 2 cut(s) 266, 598
Eco24I GRGCYC 1 cut(s) 233
Eco31I GGTCTC 1 cut(s) 654
Eco32I GATATC 1 cut(s) 388
Eco47I GGWCC 1 cut(s) 86
Eco47III AGCGCT 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 231
EcoICRI GAGCTC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 690
EcoRV GATATC 1 cut(s) 388
EcoT14I CCWWGG 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 1 cut(s) 328
FaeI CATG 1 cut(s) 426
FaiI YATR 3 cut(s) 13, 351, 424
FatI CATG 1 cut(s) 422
Fnu4HI GCNGC 3 cut(s) 175, 540, 720
FokI GGATG 3 cut(s) 130, 593, 691
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 3 cut(s) 175, 540, 720
GlaI GCGC 3 cut(s) 178, 222, 299
GluI GCNGC 3 cut(s) 175, 540, 720
HaeII RGCGCY 2 cut(s) 180, 224
HaeIII GGCC 4 cut(s) 266, 379, 476, 598
HapII CCGG 3 cut(s) 277, 288, 714
HgaI GACGC 2 cut(s) 280, 289
HhaI GCGC 3 cut(s) 179, 223, 300
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 1 cut(s) 426
Hin6I GCGC 3 cut(s) 177, 221, 298
HinP1I GCGC 3 cut(s) 177, 221, 298
HindIII AAGCTT 1 cut(s) 358
HinfI GANTC 4 cut(s) 120, 131, 163, 551
HpaII CCGG 3 cut(s) 277, 288, 714
HphI GGTGA 3 cut(s) 92, 102, 126
Hpy166II GTNNAC 2 cut(s) 503, 688
Hpy188I TCNGA 5 cut(s) 62, 148, 168, 235, 654
Hpy188III TCNNGA 5 cut(s) 128, 187, 226, 383, 602
Hpy8I GTNNAC 2 cut(s) 503, 688
Hpy99I CGWCG 3 cut(s) 193, 220, 296
HpyAV CCTTC 2 cut(s) 253, 256
HpyCH4III ACNGT 2 cut(s) 193, 500
HpyCH4IV ACGT 1 cut(s) 215
HpyCH4V TGCA 3 cut(s) 440, 503, 722
HpyF10VI GCNNNNNNNGC 1 cut(s) 297
HpyF3I CTNAG 3 cut(s) 114, 232, 603
HpySE526I ACGT 1 cut(s) 215
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 1 cut(s) 426
HspAI GCGC 3 cut(s) 177, 221, 298
Kzo9I GATC 4 cut(s) 70, 168, 332, 654
LmnI GCTCC 1 cut(s) 729
Lsp1109I GCAGC 3 cut(s) 186, 526, 706
LweI GCATC 1 cut(s) 427
MabI ACCWGGT 1 cut(s) 690
MaeII ACGT 1 cut(s) 215
MaeIII GTNAC 2 cut(s) 125, 407
MalI GATC 4 cut(s) 72, 170, 334, 656
MboI GATC 4 cut(s) 70, 168, 332, 654
MboII GAAGA 1 cut(s) 619
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 2 cut(s) 233, 505
MluCI AATT 3 cut(s) 46, 467, 485
MlyI GAGTC 3 cut(s) 114, 125, 560
MmeI TCCRAC 1 cut(s) 113
MseI TTAA 4 cut(s) 50, 341, 624, 745
MspA1I CMGCKG 1 cut(s) 405
MspI CCGG 3 cut(s) 277, 288, 714
MspR9I CCNGG 1 cut(s) 692
Mva1269I GAATGC 1 cut(s) 525
MvaI CCWGG 1 cut(s) 692
MvnI CGCG 2 cut(s) 227, 300
MwoI GCNNNNNNNGC 1 cut(s) 297
NdeII GATC 4 cut(s) 70, 168, 332, 654
NlaIII CATG 1 cut(s) 426
NlaIV GGNNCC 2 cut(s) 72, 87
NmuCI GTSAC 1 cut(s) 125
NruI TCGCGA 1 cut(s) 227
PaqCI CACCTGC 1 cut(s) 431
PceI AGGCCT 2 cut(s) 266, 598
PcsI WCGNNNNNNNCGW 1 cut(s) 224
PctI GAATGC 1 cut(s) 525
PfeI GAWTC 1 cut(s) 163
PflFI GACNNNGTC 1 cut(s) 123
PflMI CCANNNNNTGG 1 cut(s) 423
PkrI GCNGC 3 cut(s) 176, 541, 721
Ple19I CGATCG 1 cut(s) 171
PleI GAGTC 3 cut(s) 114, 125, 559
PpsI GAGTC 3 cut(s) 114, 125, 559
Psp124BI GAGCTC 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 690
PspGI CCWGG 1 cut(s) 690
PspN4I GGNNCC 2 cut(s) 72, 87
PspPI GGNCC 1 cut(s) 86
PsuI RGATCY 1 cut(s) 70
PsyI GACNNNGTC 1 cut(s) 123
PvuI CGATCG 1 cut(s) 171
RruI TCGCGA 1 cut(s) 227
RsaI GTAC 3 cut(s) 5, 644, 689
RsaNI GTAC 3 cut(s) 4, 643, 688
SacI GAGCTC 1 cut(s) 233
SaqAI TTAA 4 cut(s) 50, 341, 624, 745
SatI GCNGC 3 cut(s) 175, 540, 720
Sau3AI GATC 4 cut(s) 70, 168, 332, 654
Sau96I GGNCC 1 cut(s) 86
SchI GAGTC 3 cut(s) 114, 125, 560
ScrFI CCNGG 1 cut(s) 692
SduI GDGCHC 2 cut(s) 233, 505
SexAI ACCWGGT 1 cut(s) 690
SfaNI GCATC 1 cut(s) 427
SinI GGWCC 1 cut(s) 86
SmlI CTYRAG 3 cut(s) 281, 361, 381
SmoI CTYRAG 3 cut(s) 281, 361, 381
Sse9I AATT 3 cut(s) 46, 467, 485
SseBI AGGCCT 2 cut(s) 266, 598
SsiI CCGC 2 cut(s) 405, 523
SspI AATATT 1 cut(s) 570
SstI GAGCTC 1 cut(s) 233
StuI AGGCCT 2 cut(s) 266, 598
StyD4I CCNGG 1 cut(s) 690
StyI CCWWGG 1 cut(s) 328
TaaI ACNGT 2 cut(s) 193, 500
TaiI ACGT 1 cut(s) 218
TaqI TCGA 3 cut(s) 188, 294, 315
TasI AATT 3 cut(s) 46, 467, 485
TfiI GAWTC 1 cut(s) 163
Tru1I TTAA 4 cut(s) 50, 341, 624, 745
Tru9I TTAA 4 cut(s) 50, 341, 624, 745
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 3 cut(s) 174, 539, 719
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 1 cut(s) 235
TspGWI ACGGA 1 cut(s) 113
Tth111I GACNNNGTC 1 cut(s) 123
Van91I CCANNNNNTGG 1 cut(s) 423
VneI GTGCAC 1 cut(s) 501
VpaK11BI GGWCC 1 cut(s) 86
XapI RAATTY 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.