pycom17g01210

Ribosome-recycling factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007948
Physical Location & Seq
Reverse (-)
842714 .. 845077
2364 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 780 bp
ATGGCGATTTTTCTGAGGCGCGCGCTCTCATTCCGGAACGTCCTTCTCCTCCGTAGCTCCGCGCTGCTCAGTCACGAGCACCGGCAATGCAACTCCGCGCCACCCCACAGGGTTTTATCTCTTCCCGCCTTTGAATTCGTCAGAGAAAGCCGCAGAGGCTTCGCCAAAGGCCGCAATAAAAAAAAAGAAGATGAATCGGCTGCGAGTACAGTTGAAATTTTGCCAAACATGGGGCCGACGATCAAGGCAAATGCCACCTCGCATATGGAGGCGGCGATCGCAGCGCTAACGGCGGAATTAAGCAAACTGCGCACCGGAAGAGCATCTCCTGGAATGCTTGATCACATCATTGTGGAAACCGGCGGTGTGAAGTTGCCCTTGCATCAGATAGCCGCTGTTTCGGTCATTGATTCGAAAACTTTATCCATCAACCCTTTCGATCCAACGGCCTTATCGAACTTGGAGACTGCCATTGTTGAATCTCCATTGGGCTTAAATCCTAAAGTGGATGGGGACCGACTGATTGCAGCAATTCCTCCATTGACGAAAGAGCATGTTCAGGCTATTTGTAAAGTGGTTACCAAGTCCGCTGAAGATGGCAAACAAAGTATTCGAAGAGGTCGCCAAAAGGCAATGGATACGATAAAAAAGTTGACCAAAAACTACCCGAAGGACGATTTAAAGAGATGGGAGAAAGAGGTTGACGAGTTGACTAAAAAGTTTGTCAAGACAGCAGAGGACTTGTGCAAGGCAAAGGAGAAAGAGATCAGTTCTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRF

Protein Analysis

260

Amino Acids

28.47

Weight (kDa)

9.66

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRF PF01765 97 - 256 5.4e-53 Ribosome recycling factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 311
AccII CGCG 4 cut(s) 21, 23, 62, 98
AccIII TCCGGA 1 cut(s) 33
AclWI GGATC 1 cut(s) 434
AcsI RAATTY 2 cut(s) 134, 216
AcuI CTGAAG 1 cut(s) 612
AfaI GTAC 1 cut(s) 208
AfeI AGCGCT 1 cut(s) 285
AfiI CCNNNNNNNGG 1 cut(s) 230
AgsI TTSAA 3 cut(s) 134, 215, 479
AjnI CCWGG 1 cut(s) 328
AluBI AGCT 1 cut(s) 57
AluI AGCT 1 cut(s) 57
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 1 cut(s) 458
AlwI GGATC 1 cut(s) 434
Aor13HI TCCGGA 1 cut(s) 33
Aor51HI AGCGCT 1 cut(s) 285
AoxI GGCC 3 cut(s) 169, 233, 447
ApeKI GCWGC 4 cut(s) 64, 200, 281, 527
ApoI RAATTY 2 cut(s) 134, 216
AsiSI GCGATCGC 1 cut(s) 279
AspLEI GCGC 7 cut(s) 21, 23, 25, 64, 100, 286, 312
AspS9I GGNCC 2 cut(s) 233, 514
AsuII TTCGAA 2 cut(s) 413, 613
AvaII GGWCC 1 cut(s) 514
BauI CACGAG 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 4 cut(s) 51, 187, 293, 539
BccI CCATC 4 cut(s) 434, 503, 590, 681
BceAI ACGGC 2 cut(s) 306, 462
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BciT130I CCWGG 1 cut(s) 330
BciVI GTATCC 1 cut(s) 631
BclI TGATCA 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 458
BfoI RGCGCY 1 cut(s) 287
BfuI GTATCC 1 cut(s) 631
BglI GCCNNNNNGGC 1 cut(s) 156
BisI GCNGC 8 cut(s) 65, 151, 172, 201, 273, 282, 393, 528
BlsI GCNGC 8 cut(s) 66, 152, 173, 202, 274, 283, 394, 529
Bme1390I CCNGG 1 cut(s) 330
Bme18I GGWCC 1 cut(s) 514
BmgT120I GGNCC 2 cut(s) 233, 514
BmiI GGNNCC 2 cut(s) 234, 515
BmrFI CCNGG 1 cut(s) 330
BmsI GCATC 2 cut(s) 332, 391
Bpu14I TTCGAA 2 cut(s) 413, 613
BsaWI WCCGGW 2 cut(s) 33, 314
Bsc4I CCNNNNNNNGG 1 cut(s) 230
Bse118I RCCGGY 2 cut(s) 81, 359
Bse3DI GCAATG 2 cut(s) 92, 639
BseAI TCCGGA 1 cut(s) 33
BseBI CCWGG 1 cut(s) 330
BseGI GGATG 1 cut(s) 514
BseLI CCNNNNNNNGG 1 cut(s) 230
BseMI GCAATG 2 cut(s) 92, 639
BseMII CTCAG 2 cut(s) 5, 82
BsePI GCGCGC 2 cut(s) 19, 21
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 4 cut(s) 51, 187, 293, 539
Bsh1236I CGCG 4 cut(s) 21, 23, 62, 98
Bsh1285I CGRYCG 1 cut(s) 279
BshFI GGCC 3 cut(s) 171, 235, 449
BsiEI CGRYCG 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 4 cut(s) 34, 82, 315, 360
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 1 cut(s) 230
BsmAI GTCTC 1 cut(s) 458
BsmFI GGGAC 1 cut(s) 527
BsmI GAATGC 1 cut(s) 339
BsnI GGCC 3 cut(s) 171, 235, 449
Bsp119I TTCGAA 2 cut(s) 413, 613
Bsp1286I GDGCHC 1 cut(s) 81
Bsp13I TCCGGA 1 cut(s) 33
Bsp143I GATC 5 cut(s) 240, 276, 340, 439, 765
BspANI GGCC 3 cut(s) 171, 235, 449
BspCNI CTCAG 2 cut(s) 6, 81
BspEI TCCGGA 1 cut(s) 33
BspFNI CGCG 4 cut(s) 21, 23, 62, 98
BspLI GGNNCC 2 cut(s) 234, 515
BspPI GGATC 1 cut(s) 434
BspQI GCTCTTC 1 cut(s) 313
BspT104I TTCGAA 2 cut(s) 413, 613
BsrDI GCAATG 2 cut(s) 92, 639
BsrFI RCCGGY 2 cut(s) 81, 359
BssAI RCCGGY 2 cut(s) 81, 359
BssHII GCGCGC 2 cut(s) 19, 21
BssMI GATC 5 cut(s) 240, 276, 340, 439, 765
BssSI CACGAG 1 cut(s) 74
Bst2BI CACGAG 1 cut(s) 74
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 211
Bst6I CTCTTC 3 cut(s) 126, 313, 610
BstBI TTCGAA 2 cut(s) 413, 613
BstC8I GCNNGC 2 cut(s) 21, 23
BstDEI CTNAG 2 cut(s) 14, 68
BstEII GGTNACC 1 cut(s) 577
BstF5I GGATG 1 cut(s) 514
BstFNI CGCG 4 cut(s) 21, 23, 62, 98
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 7 cut(s) 21, 23, 25, 64, 100, 286, 312
BstKTI GATC 5 cut(s) 243, 279, 343, 442, 768
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 5 cut(s) 240, 276, 340, 439, 765
BstMCI CGRYCG 1 cut(s) 279
BstMWI GCNNNNNNNGC 5 cut(s) 156, 278, 281, 290, 309
BstNI CCWGG 1 cut(s) 330
BstNSI RCATGY 1 cut(s) 557
BstPI GGTNACC 1 cut(s) 577
BstSCI CCNGG 1 cut(s) 328
BstUI CGCG 4 cut(s) 21, 23, 62, 98
BstV1I GCAGC 4 cut(s) 51, 187, 293, 539
BsuI GTATCC 1 cut(s) 631
BsuRI GGCC 3 cut(s) 171, 235, 449
BtsCI GGATG 1 cut(s) 514
Cac8I GCNNGC 2 cut(s) 21, 23
CfoI GCGC 7 cut(s) 21, 23, 25, 64, 100, 286, 312
Cfr10I RCCGGY 2 cut(s) 81, 359
Cfr13I GGNCC 2 cut(s) 233, 514
Csp6I GTAC 1 cut(s) 207
CviAII CATG 2 cut(s) 229, 554
CviQI GTAC 1 cut(s) 207
DdeI CTNAG 2 cut(s) 14, 68
DpnI GATC 5 cut(s) 242, 278, 342, 441, 767
DpnII GATC 5 cut(s) 240, 276, 340, 439, 765
DraI TTTAAA 1 cut(s) 681
Eam1104I CTCTTC 3 cut(s) 126, 313, 610
EarI CTCTTC 3 cut(s) 126, 313, 610
EciI GGCGGA 1 cut(s) 308
Eco47I GGWCC 1 cut(s) 514
Eco47III AGCGCT 1 cut(s) 285
Eco57I CTGAAG 1 cut(s) 612
Eco91I GGTNACC 1 cut(s) 577
EcoO65I GGTNACC 1 cut(s) 577
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 328
FaeI CATG 2 cut(s) 232, 557
FaiI YATR 4 cut(s) 230, 264, 266, 555
FalI AAGNNNNNCTT 2 cut(s) 362, 394
FaqI GGGAC 1 cut(s) 527
FatI CATG 2 cut(s) 228, 553
FauI CCCGC 1 cut(s) 133
FauNDI CATATG 1 cut(s) 264
FbaI TGATCA 1 cut(s) 340
Fnu4HI GCNGC 8 cut(s) 65, 151, 172, 201, 273, 282, 393, 528
FokI GGATG 1 cut(s) 521
Fsp4HI GCNGC 8 cut(s) 65, 151, 172, 201, 273, 282, 393, 528
FspI TGCGCA 1 cut(s) 311
GlaI GCGC 7 cut(s) 20, 22, 24, 63, 99, 285, 311
GluI GCNGC 8 cut(s) 65, 151, 172, 201, 273, 282, 393, 528
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 3 cut(s) 171, 235, 449
HapII CCGG 4 cut(s) 34, 82, 315, 360
HhaI GCGC 7 cut(s) 21, 23, 25, 64, 100, 286, 312
Hin1II CATG 2 cut(s) 232, 557
Hin6I GCGC 7 cut(s) 19, 21, 23, 62, 98, 284, 310
HinP1I GCGC 7 cut(s) 19, 21, 23, 62, 98, 284, 310
HincII GTYRAC 3 cut(s) 654, 703, 711
HindII GTYRAC 3 cut(s) 654, 703, 711
HinfI GANTC 3 cut(s) 194, 410, 479
HpaII CCGG 4 cut(s) 34, 82, 315, 360
Hpy166II GTNNAC 3 cut(s) 654, 703, 711
Hpy188I TCNGA 3 cut(s) 15, 143, 387
Hpy188III TCNNGA 3 cut(s) 34, 74, 727
Hpy8I GTNNAC 3 cut(s) 654, 703, 711
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 2 cut(s) 53, 664
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 4 cut(s) 90, 382, 527, 747
HpyF10VI GCNNNNNNNGC 5 cut(s) 156, 278, 281, 290, 309
HpyF3I CTNAG 2 cut(s) 14, 68
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 2 cut(s) 232, 557
HspAI GCGC 7 cut(s) 19, 21, 23, 62, 98, 284, 310
Kpn2I TCCGGA 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 5 cut(s) 240, 276, 340, 439, 765
LguI GCTCTTC 1 cut(s) 313
LmnI GCTCC 1 cut(s) 62
LpnPI CCDG 9 cut(s) 47, 94, 95, 315, 328, 342, 373, 545, 759
Lsp1109I GCAGC 4 cut(s) 51, 187, 293, 539
LweI GCATC 2 cut(s) 332, 391
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 2 cut(s) 71, 577
MalI GATC 5 cut(s) 242, 278, 342, 441, 767
MboI GATC 5 cut(s) 240, 276, 340, 439, 765
MboII GAAGA 5 cut(s) 113, 200, 330, 605, 627
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 4 cut(s) 134, 216, 296, 531
MmeI TCCRAC 1 cut(s) 467
MnlI CCTC 9 cut(s) 9, 59, 149, 262, 268, 546, 611, 691, 730
MroI TCCGGA 1 cut(s) 33
MseI TTAA 3 cut(s) 299, 494, 680
MslI CAYNNNNRTG 1 cut(s) 350
MspA1I CMGCKG 2 cut(s) 395, 590
MspI CCGG 4 cut(s) 34, 82, 315, 360
MspR9I CCNGG 1 cut(s) 330
Mva1269I GAATGC 1 cut(s) 339
MvaI CCWGG 1 cut(s) 330
MvnI CGCG 4 cut(s) 21, 23, 62, 98
MwoI GCNNNNNNNGC 5 cut(s) 156, 278, 281, 290, 309
NdeI CATATG 1 cut(s) 264
NdeII GATC 5 cut(s) 240, 276, 340, 439, 765
NlaIII CATG 2 cut(s) 232, 557
NlaIV GGNNCC 2 cut(s) 234, 515
NmuCI GTSAC 1 cut(s) 71
NsbI TGCGCA 1 cut(s) 311
NspI RCATGY 1 cut(s) 557
NspV TTCGAA 2 cut(s) 413, 613
PauI GCGCGC 2 cut(s) 19, 21
PciSI GCTCTTC 1 cut(s) 313
PcsI WCGNNNNNNNCGW 1 cut(s) 452
PctI GAATGC 1 cut(s) 339
PfeI GAWTC 3 cut(s) 194, 410, 479
PfoI TCCNGGA 1 cut(s) 328
PkrI GCNGC 8 cut(s) 66, 152, 173, 202, 274, 283, 394, 529
Ple19I CGATCG 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 328
PspEI GGTNACC 1 cut(s) 577
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 2 cut(s) 234, 515
PspPI GGNCC 2 cut(s) 233, 514
PteI GCGCGC 2 cut(s) 19, 21
PvuI CGATCG 1 cut(s) 279
RgaI GCGATCGC 1 cut(s) 279
RsaI GTAC 1 cut(s) 208
RsaNI GTAC 1 cut(s) 207
RseI CAYNNNNRTG 1 cut(s) 350
SapI GCTCTTC 1 cut(s) 313
SaqAI TTAA 3 cut(s) 299, 494, 680
SatI GCNGC 8 cut(s) 65, 151, 172, 201, 273, 282, 393, 528
Sau3AI GATC 5 cut(s) 240, 276, 340, 439, 765
Sau96I GGNCC 2 cut(s) 233, 514
ScrFI CCNGG 1 cut(s) 330
SduI GDGCHC 1 cut(s) 81
SetI ASST 5 cut(s) 42, 59, 260, 622, 702
SfaAI GCGATCGC 1 cut(s) 279
SfaNI GCATC 2 cut(s) 332, 391
SfuI TTCGAA 2 cut(s) 413, 613
SgfI GCGATCGC 1 cut(s) 279
SinI GGWCC 1 cut(s) 514
SmiMI CAYNNNNRTG 1 cut(s) 350
Sse9I AATT 4 cut(s) 134, 216, 296, 531
StyD4I CCNGG 1 cut(s) 328
TaaI ACNGT 1 cut(s) 211
TaiI ACGT 1 cut(s) 42
TaqI TCGA 4 cut(s) 413, 438, 455, 613
TaqII GACCGA 2 cut(s) 391, 531
TasI AATT 4 cut(s) 134, 216, 296, 531
TatI WGTACW 1 cut(s) 206
TauI GCSGC 4 cut(s) 153, 174, 275, 395
TfiI GAWTC 3 cut(s) 194, 410, 479
Tru1I TTAA 3 cut(s) 299, 494, 680
Tru9I TTAA 3 cut(s) 299, 494, 680
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 4 cut(s) 64, 200, 281, 527
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 1 cut(s) 207
TspGWI ACGGA 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 514
XapI RAATTY 2 cut(s) 134, 216
XceI RCATGY 1 cut(s) 557
XcmI CCANNNNNNNNNTGG 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.