pycom17g01660

membrane protein At3g27390-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005016
Physical Location & Seq
Reverse (-)
1074000 .. 1075853
1854 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g01660.5

Sequence Viewer

Length: 1140 bp
ATGCTCTTCAAGGGGTGGCACCGTTTGTTTCATGACCTTATAGGTCGTGAAGGCCCTTTCTTGGAAACAATATGTGTTCCATTTGCGGGTCTTGCCATCCTACTCTGGCCGTTGGCTGTTGTTGGGGCAGTGTTGGGATCTATGGTGGCTAGTATCTTTCTTGGTGCTTACGCTGGAGTGATCGTTTATCAGGAATCCTCAATTTGGTCCGGGCTTTGCTATATCGTTGCATCACTGGCCATATATGATGAACACAGCAATGACATCCTTGACATGCCAGAAGGATCCTGCTTTCCAAGGCCAAAGTACCGAAAGAAGGCTGAATTGCTCCGGACCAACTCTCTCACAACCTCTTTCTCAAGGCCCGGCTCTTTTCGAAGTGCTCCCTCCCACTCAGCTTCATTCACTGGTCCTATCATTGAATTGAAGCCCCTTGAGTTCATTGATACCTTATTTAAGGAGTGTAAACACCATGGTCAAATATTGGTTGCTGACGGAACTATTACACTTCAAGACCTTGAAGACGCCAAGTCCAGTCAAGGCAACAGAGTCATTAGCATTGGCTTACCAGCATATTGCCTTCTTCAGACACTCCTGCGATCCGCAAAAGCCAATTCACCGGGAATATTGTTAAGTGACAATGTTACCGAGATAACTAGCACAAACAGACCAAAAGAAAAATTTTTTGATTGGTTTTTCAATCCGCTGTTGATCATCAAGGACCAGATAAAAGCAGAAAACCTTTCCGAAGCAGAAGAGGCTTACCTTTGCAAATTAGTTCTGCTGACTGGGGATCCTTTGAGGTTGAAACAATCGAACATTGGCACACCACCGGAATCAGAGCGTAAACAGGCTGAACTTGATGCATTGGCTCGAAGACTCCAAGGTATCACTAAATCTATCTCGAGGTATCCAACTTTCAGGCGCCGATTTGAGAATCTTGTCGACGCCATCTCCAATGATCTTGCACCGAATAGTAACGGTAGCAGCAAATCGAGCAATGGACCTAGAACAGTTCCGAGGTCAAAGAGCGCCTTTGCCCAGCTCTTCAGCCAGAAATCCTTTAGTTTTAGCAACGCAAGCAACCATGGGACTGATCCAGAGTCACGGCTAGTTGTAAGGGACGTGACAATCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

380

Amino Acids

41.87

Weight (kDa)

8.65

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 18, 924
AccI GTMKAC 1 cut(s) 945
AccIII TCCGGA 1 cut(s) 330
AciI CCGC 3 cut(s) 86, 603, 704
AclWI GGATC 7 cut(s) 145, 279, 292, 594, 788, 801, 1091
AcoI YGGCCR 2 cut(s) 107, 237
AcsI RAATTY 1 cut(s) 680
AcuI CTGAAG 2 cut(s) 569, 1033
AcyI GRCGYC 3 cut(s) 525, 925, 948
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 4 cut(s) 61, 86, 204, 316
AgsI TTSAA 7 cut(s) 10, 422, 427, 512, 521, 700, 808
AhdI GACNNNNNGTC 1 cut(s) 529
AjiI CACGTC 1 cut(s) 1126
AluBI AGCT 2 cut(s) 398, 1045
AluI AGCT 2 cut(s) 398, 1045
Alw21I GWGCWC 1 cut(s) 385
AlwI GGATC 7 cut(s) 145, 279, 292, 594, 788, 801, 1091
Ama87I CYCGRG 1 cut(s) 904
Aor13HI TCCGGA 1 cut(s) 330
AoxI GGCC 5 cut(s) 52, 107, 237, 299, 362
ApeKI GCWGC 1 cut(s) 987
ApoI RAATTY 1 cut(s) 680
AspLEI GCGC 2 cut(s) 927, 1034
AspS9I GGNCC 7 cut(s) 53, 207, 333, 363, 410, 721, 1004
AsuC2I CCSGG 3 cut(s) 211, 366, 621
AsuHPI GGTGA 1 cut(s) 609
AsuII TTCGAA 1 cut(s) 376
AvaI CYCGRG 1 cut(s) 904
AvaII GGWCC 5 cut(s) 207, 333, 410, 721, 1004
BalI TGGCCA 1 cut(s) 239
BamHI GGATCC 2 cut(s) 284, 793
BanI GGYRCC 2 cut(s) 18, 924
BbsI GAAGAC 2 cut(s) 528, 883
Bbv12I GWGCWC 1 cut(s) 385
BbvI GCAGC 1 cut(s) 999
BccI CCATC 2 cut(s) 104, 959
BceAI ACGGC 2 cut(s) 94, 1124
BciVI GTATCC 1 cut(s) 921
BclI TGATCA 1 cut(s) 711
BcnI CCSGG 3 cut(s) 211, 366, 621
BfaI CTAG 4 cut(s) 150, 657, 1008, 1112
BfoI RGCGCY 2 cut(s) 928, 1035
BfuI GTATCC 1 cut(s) 921
BisI GCNGC 1 cut(s) 988
BlsI GCNGC 1 cut(s) 989
Bme1390I CCNGG 3 cut(s) 211, 366, 621
Bme18I GGWCC 5 cut(s) 207, 333, 410, 721, 1004
BmeRI GACNNNNNGTC 1 cut(s) 529
BmeT110I CYCGRG 1 cut(s) 904
BmgBI CACGTC 1 cut(s) 1126
BmgT120I GGNCC 7 cut(s) 53, 207, 333, 363, 410, 721, 1004
BmiI GGNNCC 4 cut(s) 20, 286, 795, 926
BmrFI CCNGG 3 cut(s) 211, 366, 621
BmrI ACTGGG 1 cut(s) 798
BmsI GCATC 2 cut(s) 239, 853
BmuI ACTGGG 1 cut(s) 798
BpiI GAAGAC 2 cut(s) 528, 883
BpmI CTGGAG 1 cut(s) 195
Bpu14I TTCGAA 1 cut(s) 376
BpuEI CTTGAG 2 cut(s) 343, 455
BpuMI CCSGG 3 cut(s) 211, 366, 621
BsaHI GRCGYC 3 cut(s) 525, 925, 948
BsaJI CCNNGG 5 cut(s) 296, 472, 883, 1019, 1087
BsaWI WCCGGW 2 cut(s) 330, 832
BsaXI ACNNNNNCTCC 4 cut(s) 168, 198, 938, 968
Bsc4I CCNNNNNNNGG 4 cut(s) 61, 86, 204, 316
Bse1I ACTGG 4 cut(s) 240, 412, 534, 793
Bse3DI GCAATG 2 cut(s) 265, 1006
BseAI TCCGGA 1 cut(s) 330
BseDI CCNNGG 5 cut(s) 296, 472, 883, 1019, 1087
BseGI GGATG 2 cut(s) 96, 264
BseLI CCNNNNNNNGG 4 cut(s) 61, 86, 204, 316
BseMI GCAATG 2 cut(s) 265, 1006
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 4 cut(s) 240, 412, 534, 793
BseXI GCAGC 1 cut(s) 999
BseYI CCCAGC 1 cut(s) 1041
BshFI GGCC 5 cut(s) 54, 109, 239, 301, 364
BshNI GGYRCC 2 cut(s) 18, 924
BsiHKAI GWGCWC 1 cut(s) 385
BsiHKCI CYCGRG 1 cut(s) 904
BsiSI CCGG 5 cut(s) 210, 331, 366, 620, 833
BslFI GGGAC 2 cut(s) 1105, 1136
BslI CCNNNNNNNGG 4 cut(s) 61, 86, 204, 316
BsmFI GGGAC 2 cut(s) 1105, 1136
BsnI GGCC 5 cut(s) 54, 109, 239, 301, 364
BsoBI CYCGRG 1 cut(s) 904
Bsp119I TTCGAA 1 cut(s) 376
Bsp1286I GDGCHC 1 cut(s) 385
Bsp13I TCCGGA 1 cut(s) 330
Bsp143I GATC 8 cut(s) 137, 180, 284, 599, 711, 793, 961, 1096
Bsp19I CCATGG 2 cut(s) 472, 1087
BspACI CCGC 3 cut(s) 86, 603, 704
BspANI GGCC 5 cut(s) 54, 109, 239, 301, 364
BspCNI CTCAG 1 cut(s) 407
BspEI TCCGGA 1 cut(s) 330
BspHI TCATGA 1 cut(s) 31
BspLI GGNNCC 4 cut(s) 20, 286, 795, 926
BspPI GGATC 7 cut(s) 145, 279, 292, 594, 788, 801, 1091
BspQI GCTCTTC 2 cut(s) 11, 1052
BspT104I TTCGAA 1 cut(s) 376
BspT107I GGYRCC 2 cut(s) 18, 924
BsrDI GCAATG 2 cut(s) 265, 1006
BsrI ACTGG 4 cut(s) 240, 412, 534, 793
BssECI CCNNGG 5 cut(s) 296, 472, 883, 1019, 1087
BssMI GATC 8 cut(s) 137, 180, 284, 599, 711, 793, 961, 1096
BssNI GRCGYC 3 cut(s) 525, 925, 948
BssT1I CCWWGG 4 cut(s) 296, 472, 883, 1087
Bst4CI ACNGT 3 cut(s) 23, 983, 1015
Bst6I CTCTTC 3 cut(s) 11, 750, 1052
BstACI GRCGYC 3 cut(s) 525, 925, 948
BstBI TTCGAA 1 cut(s) 376
BstC8I GCNNGC 1 cut(s) 1081
BstDEI CTNAG 1 cut(s) 394
BstDSI CCRYGG 2 cut(s) 472, 1087
BstF5I GGATG 2 cut(s) 96, 264
BstH2I RGCGCY 2 cut(s) 928, 1035
BstHHI GCGC 2 cut(s) 927, 1034
BstKTI GATC 8 cut(s) 140, 183, 287, 602, 714, 796, 964, 1099
BstMBI GATC 8 cut(s) 137, 180, 284, 599, 711, 793, 961, 1096
BstMWI GCNNNNNNNGC 5 cut(s) 92, 236, 758, 996, 1080
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 3 cut(s) 209, 364, 619
BstV1I GCAGC 1 cut(s) 999
BstV2I GAAGAC 2 cut(s) 528, 883
BstX2I RGATCY 3 cut(s) 137, 284, 793
BstYI RGATCY 3 cut(s) 137, 284, 793
BsuI GTATCC 1 cut(s) 921
BsuRI GGCC 5 cut(s) 54, 109, 239, 301, 364
BtgI CCRYGG 2 cut(s) 472, 1087
BtrI CACGTC 1 cut(s) 1126
BtsCI GGATG 2 cut(s) 96, 264
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 3 cut(s) 135, 233, 405
Cac8I GCNNGC 1 cut(s) 1081
CciI TCATGA 1 cut(s) 31
CfoI GCGC 2 cut(s) 927, 1034
Cfr13I GGNCC 7 cut(s) 53, 207, 333, 363, 410, 721, 1004
CseI GACGC 2 cut(s) 533, 956
Csp6I GTAC 1 cut(s) 307
CviAII CATG 4 cut(s) 32, 274, 473, 1088
CviQI GTAC 1 cut(s) 307
DdeI CTNAG 1 cut(s) 394
DinI GGCGCC 1 cut(s) 926
DpnI GATC 8 cut(s) 139, 182, 286, 601, 713, 795, 963, 1098
DpnII GATC 8 cut(s) 137, 180, 284, 599, 711, 793, 961, 1096
DriI GACNNNNNGTC 1 cut(s) 529
EaeI YGGCCR 2 cut(s) 107, 237
Eam1104I CTCTTC 3 cut(s) 11, 750, 1052
Eam1105I GACNNNNNGTC 1 cut(s) 529
EarI CTCTTC 3 cut(s) 11, 750, 1052
Eco130I CCWWGG 4 cut(s) 296, 472, 883, 1087
Eco47I GGWCC 5 cut(s) 207, 333, 410, 721, 1004
Eco57I CTGAAG 2 cut(s) 569, 1033
Eco88I CYCGRG 1 cut(s) 904
EcoO109I RGGNCCY 1 cut(s) 53
EcoT14I CCWWGG 4 cut(s) 296, 472, 883, 1087
EcoT22I ATGCAT 1 cut(s) 868
EgeI GGCGCC 1 cut(s) 926
EheI GGCGCC 1 cut(s) 926
ErhI CCWWGG 4 cut(s) 296, 472, 883, 1087
FaeI CATG 4 cut(s) 35, 277, 476, 1091
FalI AAGNNNNNCTT 2 cut(s) 1019, 1051
FaqI GGGAC 2 cut(s) 1105, 1136
FatI CATG 4 cut(s) 31, 273, 472, 1087
FauI CCCGC 1 cut(s) 79
FbaI TGATCA 1 cut(s) 711
FblI GTMKAC 1 cut(s) 945
Fnu4HI GCNGC 1 cut(s) 988
FokI GGATG 2 cut(s) 83, 251
Fsp4HI GCNGC 1 cut(s) 988
FspBI CTAG 4 cut(s) 150, 657, 1008, 1112
GlaI GCGC 2 cut(s) 926, 1033
GluI GCNGC 1 cut(s) 988
GsaI CCCAGC 1 cut(s) 1045
GsuI CTGGAG 1 cut(s) 195
HaeII RGCGCY 2 cut(s) 928, 1035
HaeIII GGCC 5 cut(s) 54, 109, 239, 301, 364
HapII CCGG 5 cut(s) 210, 331, 366, 620, 833
HgaI GACGC 2 cut(s) 533, 956
HhaI GCGC 2 cut(s) 927, 1034
Hin1I GRCGYC 3 cut(s) 525, 925, 948
Hin1II CATG 4 cut(s) 35, 277, 476, 1091
Hin6I GCGC 2 cut(s) 925, 1032
HinP1I GCGC 2 cut(s) 925, 1032
HincII GTYRAC 1 cut(s) 946
HindII GTYRAC 1 cut(s) 946
HinfI GANTC 6 cut(s) 194, 549, 836, 879, 937, 1103
HpaII CCGG 5 cut(s) 210, 331, 366, 620, 833
HphI GGTGA 1 cut(s) 609
Hpy166II GTNNAC 3 cut(s) 467, 848, 946
Hpy188I TCNGA 4 cut(s) 588, 748, 841, 1020
Hpy188III TCNNGA 7 cut(s) 32, 47, 191, 331, 512, 904, 1100
Hpy8I GTNNAC 3 cut(s) 467, 848, 946
Hpy99I CGWCG 1 cut(s) 950
HpyAV CCTTC 4 cut(s) 44, 275, 310, 590
HpyCH4III ACNGT 3 cut(s) 23, 983, 1015
HpyCH4IV ACGT 1 cut(s) 1125
HpyCH4V TGCA 4 cut(s) 230, 771, 866, 968
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 236, 758, 996, 1080
HpyF3I CTNAG 1 cut(s) 394
HpySE526I ACGT 1 cut(s) 1125
Hsp92I GRCGYC 3 cut(s) 525, 925, 948
Hsp92II CATG 4 cut(s) 35, 277, 476, 1091
HspAI GCGC 2 cut(s) 925, 1032
KasI GGCGCC 1 cut(s) 924
Kpn2I TCCGGA 1 cut(s) 330
Ksp22I TGATCA 1 cut(s) 711
Kzo9I GATC 8 cut(s) 137, 180, 284, 599, 711, 793, 961, 1096
LguI GCTCTTC 2 cut(s) 11, 1052
LmnI GCTCC 2 cut(s) 333, 388
Lsp1109I GCAGC 1 cut(s) 999
LweI GCATC 2 cut(s) 239, 853
MaeI CTAG 4 cut(s) 150, 657, 1008, 1112
MaeII ACGT 1 cut(s) 1125
MaeIII GTNAC 5 cut(s) 635, 643, 977, 1104, 1126
MalI GATC 8 cut(s) 139, 182, 286, 601, 713, 795, 963, 1098
MboI GATC 8 cut(s) 137, 180, 284, 599, 711, 793, 961, 1096
MboII GAAGA 5 cut(s) 533, 575, 767, 888, 1039
MflI RGATCY 3 cut(s) 137, 284, 793
MhlI GDGCHC 1 cut(s) 385
MlsI TGGCCA 1 cut(s) 239
MluCI AATT 6 cut(s) 201, 323, 422, 613, 680, 773
MluNI TGGCCA 1 cut(s) 239
Mly113I GGCGCC 1 cut(s) 925
MlyI GAGTC 3 cut(s) 558, 873, 1112
MmeI TCCRAC 1 cut(s) 938
MnlI CCTC 7 cut(s) 208, 361, 397, 751, 795, 900, 1014
Mox20I TGGCCA 1 cut(s) 239
Mph1103I ATGCAT 1 cut(s) 868
MroI TCCGGA 1 cut(s) 330
MscI TGGCCA 1 cut(s) 239
MseI TTAA 2 cut(s) 456, 632
MslI CAYNNNNRTG 1 cut(s) 258
Msp20I TGGCCA 1 cut(s) 239
MspA1I CMGCKG 1 cut(s) 706
MspI CCGG 5 cut(s) 210, 331, 366, 620, 833
MspR9I CCNGG 3 cut(s) 211, 366, 621
MwoI GCNNNNNNNGC 5 cut(s) 92, 236, 758, 996, 1080
NarI GGCGCC 1 cut(s) 925
NciI CCSGG 3 cut(s) 211, 366, 621
NcoI CCATGG 2 cut(s) 472, 1087
NdeII GATC 8 cut(s) 137, 180, 284, 599, 711, 793, 961, 1096
NlaIII CATG 4 cut(s) 35, 277, 476, 1091
NlaIV GGNNCC 4 cut(s) 20, 286, 795, 926
NmuCI GTSAC 3 cut(s) 635, 1104, 1126
NsiI ATGCAT 1 cut(s) 868
NspI RCATGY 1 cut(s) 277
NspV TTCGAA 1 cut(s) 376
PaeR7I CTCGAG 1 cut(s) 904
PagI TCATGA 1 cut(s) 31
PciSI GCTCTTC 2 cut(s) 11, 1052
PfeI GAWTC 3 cut(s) 194, 836, 937
PkrI GCNGC 1 cut(s) 989
PleI GAGTC 3 cut(s) 557, 873, 1111
PluTI GGCGCC 1 cut(s) 928
PpsI GAGTC 3 cut(s) 557, 873, 1111
PspFI CCCAGC 1 cut(s) 1041
PspN4I GGNNCC 4 cut(s) 20, 286, 795, 926
PspPI GGNCC 7 cut(s) 53, 207, 333, 363, 410, 721, 1004
PsuI RGATCY 3 cut(s) 137, 284, 793
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
RseI CAYNNNNRTG 1 cut(s) 258
SalI GTCGAC 1 cut(s) 944
SapI GCTCTTC 2 cut(s) 11, 1052
SaqAI TTAA 2 cut(s) 456, 632
SatI GCNGC 1 cut(s) 988
Sau3AI GATC 8 cut(s) 137, 180, 284, 599, 711, 793, 961, 1096
Sau96I GGNCC 7 cut(s) 53, 207, 333, 363, 410, 721, 1004
SchI GAGTC 3 cut(s) 558, 873, 1112
ScrFI CCNGG 3 cut(s) 211, 366, 621
SduI GDGCHC 1 cut(s) 385
SfaNI GCATC 2 cut(s) 239, 853
SfoI GGCGCC 1 cut(s) 926
Sfr274I CTCGAG 1 cut(s) 904
SfuI TTCGAA 1 cut(s) 376
SinI GGWCC 5 cut(s) 207, 333, 410, 721, 1004
SlaI CTCGAG 1 cut(s) 904
SmiMI CAYNNNNRTG 1 cut(s) 258
SmlI CTYRAG 3 cut(s) 358, 434, 904
SmoI CTYRAG 3 cut(s) 358, 434, 904
Sse9I AATT 6 cut(s) 201, 323, 422, 613, 680, 773
SsiI CCGC 3 cut(s) 86, 603, 704
SspDI GGCGCC 1 cut(s) 924
SspI AATATT 2 cut(s) 483, 627
SspMI CTAG 4 cut(s) 150, 657, 1008, 1112
StyD4I CCNGG 3 cut(s) 209, 364, 619
StyI CCWWGG 4 cut(s) 296, 472, 883, 1087
TaaI ACNGT 3 cut(s) 23, 983, 1015
TaiI ACGT 1 cut(s) 1128
TaqI TCGA 6 cut(s) 376, 815, 874, 905, 945, 995
TasI AATT 6 cut(s) 201, 323, 422, 613, 680, 773
TfiI GAWTC 3 cut(s) 194, 836, 937
Tru1I TTAA 2 cut(s) 456, 632
Tru9I TTAA 2 cut(s) 456, 632
TscAI CASTG 3 cut(s) 135, 240, 412
TseFI GTSAC 3 cut(s) 635, 1104, 1126
TseI GCWGC 1 cut(s) 987
Tsp45I GTSAC 3 cut(s) 635, 1104, 1126
TspDTI ATGAA 4 cut(s) 20, 264, 390, 430
TspGWI ACGGA 1 cut(s) 510
TspRI CASTG 3 cut(s) 135, 240, 412
VpaK11BI GGWCC 5 cut(s) 207, 333, 410, 721, 1004
XapI RAATTY 1 cut(s) 680
XceI RCATGY 1 cut(s) 277
XhoI CTCGAG 1 cut(s) 904
XmiI GTMKAC 1 cut(s) 945
XspI CTAG 4 cut(s) 150, 657, 1008, 1112
Zsp2I ATGCAT 1 cut(s) 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.