Rorug02G0572700

membrane protein At3g27390-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005016
Physical Location & Seq
Forward (+)
69872476 .. 69876170
3695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0572700.1

Sequence Viewer

Length: 834 bp
ATGATTCTCATCCACTTGGAAATGGGGTTATATGAAAGTTGTATAATGAGTAGTTGTGTGAAACCTCGAGTACAAACCAAGAACAATCCAGTGGTTCTTCTCCATTGCTTTGACAGTTCTTGTTTAGAATGGAGGTGCACATATCCCCTGCTTGAAGAGGCCGGGTTGGAGGCTTGGGCGATTGATGTGCTTGGGTGGGGCTTCTCCGATTTAGAAAGGCTTCCACCATGCAATCCGGAATCAAAGCGTCATCACCTCTATCAGTTTTGGAAGTCCTACATCAGAAGGCCGATGATATTAGTTGGACCAAGCCTCGGTGCTTCTGTTGCAATTGACTTTGCAGTTAGTTATCCATTTGCTGTTGAAAAGCTGGTTTTGATTAATGCAAGTGTGTACGCAGAAGGCACCAAAGGCCTTGCAGAATTTCCTAGAGTATTAGCCTATGCTGGGGTTTCCTTGTTGAGGAGCATCCCTCTACGGCTTTACGCCAATACGCTGGCCTTCAATGACATTTCATTAGCTACAACCTTTGATTGGACTAATGTCGGTCGCTTACACTGTTTGCTGCCTTGGTGGAAAGATGCAACAGTAAGTTTCATGAGTAGTGGGGGCTACAATGTTATTGCGCAAATAAAACAGGTAAAGCAGAAAGCACTTGTCATCTGTAGTGAGAAAGATCGGATTATCGACTACAAGCAAACACTGAGACTACGGTGTGAACTACCCTCTGCAATCATGCGGCTAATACCGGATGGTGGTCATCTTCCTCATGTTGACAATCCCACCTCTGTTGCAAAGCTAATTGCAGGGTTTGCTCACAATGACTGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

277

Amino Acids

31.06

Weight (kDa)

7.98

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 29 - 129 5.8e-06 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 31 - 266 7.9e-15 Alpha/beta hydrolase family
Hydrolase_4 PF12146 31 - 255 4e-09 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 627
AccB1I GGYRCC 1 cut(s) 404
AccIII TCCGGA 1 cut(s) 235
AciI CCGC 1 cut(s) 739
AcsI RAATTY 1 cut(s) 422
AfaI GTAC 2 cut(s) 72, 395
AfiI CCNNNNNNNGG 5 cut(s) 314, 447, 462, 534, 755
AgsI TTSAA 3 cut(s) 155, 365, 505
AluBI AGCT 3 cut(s) 370, 521, 799
AluI AGCT 3 cut(s) 370, 521, 799
Alw21I GWGCWC 1 cut(s) 140
Alw26I GTCTC 1 cut(s) 700
Alw44I GTGCAC 1 cut(s) 136
Ama87I CYCGRG 1 cut(s) 66
Aor13HI TCCGGA 1 cut(s) 235
AoxI GGCC 4 cut(s) 159, 287, 412, 498
ApaLI GTGCAC 1 cut(s) 136
ApeKI GCWGC 1 cut(s) 565
ApoI RAATTY 1 cut(s) 422
AseI ATTAAT 1 cut(s) 381
Asp700I GAANNNNTTC 1 cut(s) 219
AspLEI GCGC 1 cut(s) 628
AspS9I GGNCC 1 cut(s) 305
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 1 cut(s) 245
AvaI CYCGRG 1 cut(s) 66
AvaII GGWCC 1 cut(s) 305
BaeGI GKGCMC 1 cut(s) 140
BanI GGYRCC 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 140
BbvI GCAGC 1 cut(s) 552
BccI CCATC 1 cut(s) 746
BceAI ACGGC 1 cut(s) 494
BcgI CGANNNNNNTGC 2 cut(s) 169, 203
BcnI CCSGG 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 700
BfaI CTAG 1 cut(s) 429
BfmI CTRYAG 1 cut(s) 664
BisI GCNGC 2 cut(s) 566, 740
BlsI GCNGC 2 cut(s) 567, 741
Bme1390I CCNGG 1 cut(s) 163
Bme18I GGWCC 1 cut(s) 305
BmeT110I CYCGRG 1 cut(s) 66
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 1 cut(s) 163
BmsI GCATC 2 cut(s) 477, 571
BoxI GACNNNNGTC 1 cut(s) 542
BplI GAGNNNNNCTC 2 cut(s) 457, 489
BpuMI CCSGG 1 cut(s) 163
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 313, 569
BsaWI WCCGGW 2 cut(s) 235, 748
Bsc4I CCNNNNNNNGG 5 cut(s) 314, 447, 462, 534, 755
Bse1I ACTGG 1 cut(s) 89
Bse3DI GCAATG 1 cut(s) 103
Bse8I GATNNNNATC 1 cut(s) 8
BseAI TCCGGA 1 cut(s) 235
BseDI CCNNGG 2 cut(s) 313, 569
BseGI GGATG 3 cut(s) 9, 468, 757
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 5 cut(s) 314, 447, 462, 534, 755
BseMI GCAATG 1 cut(s) 103
BseMII CTCAG 1 cut(s) 695
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 1 cut(s) 478
BseSI GKGCMC 1 cut(s) 140
BseXI GCAGC 1 cut(s) 552
BseYI CCCAGC 1 cut(s) 446
Bsh1285I CGRYCG 1 cut(s) 550
BshFI GGCC 4 cut(s) 161, 289, 414, 500
BshNI GGYRCC 1 cut(s) 404
BsiEI CGRYCG 1 cut(s) 550
BsiHKAI GWGCWC 1 cut(s) 140
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 3 cut(s) 162, 236, 749
BslI CCNNNNNNNGG 5 cut(s) 314, 447, 462, 534, 755
BsmAI GTCTC 1 cut(s) 700
BsnI GGCC 4 cut(s) 161, 289, 414, 500
BsoBI CYCGRG 1 cut(s) 66
Bsp1286I GDGCHC 1 cut(s) 140
Bsp13I TCCGGA 1 cut(s) 235
Bsp143I GATC 1 cut(s) 676
BspACI CCGC 1 cut(s) 739
BspANI GGCC 4 cut(s) 161, 289, 414, 500
BspCNI CTCAG 1 cut(s) 696
BspEI TCCGGA 1 cut(s) 235
BspHI TCATGA 1 cut(s) 597
BspLI GGNNCC 1 cut(s) 406
BspT107I GGYRCC 1 cut(s) 404
BsrDI GCAATG 1 cut(s) 103
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 313, 569
BssMI GATC 1 cut(s) 676
BssT1I CCWWGG 1 cut(s) 569
Bst4CI ACNGT 5 cut(s) 116, 560, 589, 714, 827
Bst6I CTCTTC 1 cut(s) 150
BstAPI GCANNNNNTGC 1 cut(s) 812
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 1 cut(s) 704
BstENI CCTNNNNNAGG 1 cut(s) 460
BstF5I GGATG 3 cut(s) 9, 468, 757
BstHHI GCGC 1 cut(s) 628
BstKTI GATC 1 cut(s) 679
BstMAI GTCTC 1 cut(s) 700
BstMBI GATC 1 cut(s) 676
BstMCI CGRYCG 1 cut(s) 550
BstMWI GCNNNNNNNGC 3 cut(s) 326, 411, 812
BstPAI GACNNNNGTC 1 cut(s) 542
BstSCI CCNGG 1 cut(s) 161
BstSFI CTRYAG 1 cut(s) 664
BstSLI GKGCMC 1 cut(s) 140
BstV1I GCAGC 1 cut(s) 552
BstXI CCANNNNNNTGG 1 cut(s) 496
BsuRI GGCC 4 cut(s) 161, 289, 414, 500
BtsCI GGATG 3 cut(s) 9, 468, 757
BtsIMutI CAGTG 3 cut(s) 96, 556, 701
Cac8I GCNNGC 1 cut(s) 498
CciI TCATGA 1 cut(s) 597
CfoI GCGC 1 cut(s) 628
Cfr13I GGNCC 1 cut(s) 305
CseI GACGC 1 cut(s) 236
Csp6I GTAC 2 cut(s) 71, 394
CspCI CAANNNNNGTGG 2 cut(s) 772, 807
CviAII CATG 4 cut(s) 228, 598, 736, 770
CviQI GTAC 2 cut(s) 71, 394
DdeI CTNAG 1 cut(s) 704
DpnI GATC 1 cut(s) 678
DpnII GATC 1 cut(s) 676
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
Eco130I CCWWGG 1 cut(s) 569
Eco147I AGGCCT 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 66
EcoNI CCTNNNNNAGG 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 569
ErhI CCWWGG 1 cut(s) 569
FaeI CATG 4 cut(s) 231, 601, 739, 773
FaiI YATR 9 cut(s) 31, 33, 44, 142, 229, 444, 599, 737, 771
FatI CATG 4 cut(s) 227, 597, 735, 769
Fnu4HI GCNGC 2 cut(s) 566, 740
FokI GGATG 2 cut(s) 455, 764
Fsp4HI GCNGC 2 cut(s) 566, 740
FspBI CTAG 1 cut(s) 429
FspI TGCGCA 1 cut(s) 627
GlaI GCGC 1 cut(s) 627
GluI GCNGC 2 cut(s) 566, 740
GsaI CCCAGC 1 cut(s) 450
HaeIII GGCC 4 cut(s) 161, 289, 414, 500
HapII CCGG 3 cut(s) 162, 236, 749
HgaI GACGC 1 cut(s) 236
HhaI GCGC 1 cut(s) 628
Hin1II CATG 4 cut(s) 231, 601, 739, 773
Hin6I GCGC 1 cut(s) 626
HinP1I GCGC 1 cut(s) 626
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HinfI GANTC 2 cut(s) 4, 239
HpaII CCGG 3 cut(s) 162, 236, 749
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 4 cut(s) 138, 394, 719, 775
Hpy188I TCNGA 3 cut(s) 208, 284, 681
Hpy188III TCNNGA 2 cut(s) 236, 598
Hpy8I GTNNAC 4 cut(s) 138, 394, 719, 775
HpyAV CCTTC 3 cut(s) 279, 395, 511
HpyCH4III ACNGT 5 cut(s) 116, 560, 589, 714, 827
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 411, 812
HpyF3I CTNAG 1 cut(s) 704
Hsp92II CATG 4 cut(s) 231, 601, 739, 773
HspAI GCGC 1 cut(s) 626
Kpn2I TCCGGA 1 cut(s) 235
Kzo9I GATC 1 cut(s) 676
LmnI GCTCC 1 cut(s) 465
Lsp1109I GCAGC 1 cut(s) 552
LweI GCATC 2 cut(s) 477, 571
MaeI CTAG 1 cut(s) 429
MalI GATC 1 cut(s) 678
MboI GATC 1 cut(s) 676
MboII GAAGA 3 cut(s) 89, 167, 755
MfeI CAATTG 1 cut(s) 330
MhlI GDGCHC 1 cut(s) 140
MluCI AATT 3 cut(s) 330, 422, 801
MmeI TCCRAC 2 cut(s) 147, 283
MroI TCCGGA 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 219
MseI TTAA 1 cut(s) 381
MspI CCGG 3 cut(s) 162, 236, 749
MspR9I CCNGG 1 cut(s) 163
MunI CAATTG 1 cut(s) 330
MwoI GCNNNNNNNGC 3 cut(s) 326, 411, 812
NciI CCSGG 1 cut(s) 163
NdeII GATC 1 cut(s) 676
NlaIII CATG 4 cut(s) 231, 601, 739, 773
NlaIV GGNNCC 1 cut(s) 406
NsbI TGCGCA 1 cut(s) 627
PaeR7I CTCGAG 1 cut(s) 66
PagI TCATGA 1 cut(s) 597
PceI AGGCCT 1 cut(s) 414
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 2 cut(s) 4, 239
PkrI GCNGC 2 cut(s) 567, 741
PshAI GACNNNNGTC 1 cut(s) 542
PshBI ATTAAT 1 cut(s) 381
PspFI CCCAGC 1 cut(s) 446
PspN4I GGNNCC 1 cut(s) 406
PspPI GGNCC 1 cut(s) 305
PspXI VCTCGAGB 1 cut(s) 66
RsaI GTAC 2 cut(s) 72, 395
RsaNI GTAC 2 cut(s) 71, 394
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 2 cut(s) 566, 740
Sau3AI GATC 1 cut(s) 676
Sau96I GGNCC 1 cut(s) 305
ScrFI CCNGG 1 cut(s) 163
SduI GDGCHC 1 cut(s) 140
SetI ASST 9 cut(s) 67, 137, 258, 372, 523, 530, 642, 788, 801
SfaNI GCATC 2 cut(s) 477, 571
SfcI CTRYAG 1 cut(s) 664
Sfr274I CTCGAG 1 cut(s) 66
SinI GGWCC 1 cut(s) 305
SlaI CTCGAG 1 cut(s) 66
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
Sse9I AATT 3 cut(s) 330, 422, 801
SseBI AGGCCT 1 cut(s) 414
SsiI CCGC 1 cut(s) 739
SspMI CTAG 1 cut(s) 429
StuI AGGCCT 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 161
StyI CCWWGG 1 cut(s) 569
TaaI ACNGT 5 cut(s) 116, 560, 589, 714, 827
TaqI TCGA 2 cut(s) 67, 687
TaqII GACCGA 1 cut(s) 536
TasI AATT 3 cut(s) 330, 422, 801
TatI WGTACW 1 cut(s) 70
TauI GCSGC 1 cut(s) 742
TfiI GAWTC 2 cut(s) 4, 239
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 3 cut(s) 96, 563, 708
TseI GCWGC 1 cut(s) 565
TspDTI ATGAA 3 cut(s) 48, 504, 586
TspRI CASTG 3 cut(s) 96, 563, 708
VneI GTGCAC 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 305
VspI ATTAAT 1 cut(s) 381
XagI CCTNNNNNAGG 1 cut(s) 460
XapI RAATTY 1 cut(s) 422
XhoI CTCGAG 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.