Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0011191

Physical Location & Seq

Genomic Coordinates Reverse (-)

1244854 .. 1247761

Gene Structure 2908 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2019 bp

ATGTTCGCTGCGAGGAAGCTCAACAGCCACAGCTTCAAGTCCGCCTCTTCTCTCCTCCAATTCAATCCCTCCAAAAATGCCTTGTTAAGAGATGTCTCGGCGAAGGTTTTATCAAGCAATGTACTTCAGCCAAGTAAGCCGAGTGACGATTGTTTCTCTTCTTCATCGAGACCAGCCAATCCCTTCGATGGGTGGTCTAGATCGATCCCGACATCTAGAGGGAGTAGTAGCATGAGGAGCAAAGTCGCTAAGCGGATGCAAAAGGAGTCTGGTAAGACTCTAAGAGAGGTTCGCAGGGCGAAAAAGCTTCAAAAGAAGCTGATGACCGAAAATGAGAGGCTCATTTACAACCTCAAAAGAGCCAAGAGGAAAGTAGCATTACTGCTACAAAAGCTGAAAAAGTACGAGCTTCCAGATCTGCCCTCACCCCGTCATGATCCTGAGCTAGTAACCCTCGAGCAGCTTCAGGCATTGAAGAAGATTGGCTTCAAAAATAAAAATTATGTTCCTGTCGGTGTTCGCGGGGTCTTTGGAGGAGTTGTCCAGAATATGCATCTACACTGGAAGTTTCACGAGACTGTACAAGTTTGCTGTGATAACTTCCCCAAAGAAAAGATCAAGGAAATGGCCTCTATGATTGCGAGACTGAGTGGTGGTATAGTGGTAAACATACACAATACTAAAACAATCATCATGTTTCGCGGAAGAAATTACCGGCAGCCCAAAAATTTAATACCTTTCAATACCCTTACAAAAAGGAAGGCATTATTCAAGGCAAGATTTGAACAAGCCCTCGAATCTCAGAAGCTAAACATAAAGAAGATAGAACAGCAGCTCCGCCAAAGTGGTATTAATCCTGAGGATCCAGTTGCCATTGCAAGCATCCAGAGAGTAGCTTCCTCGTTCTTTAATGCATTTGATCAGAAGGACGGAAGTCCTTATGTCTTCCATGGGGACAAACCGTCTGTAGCAGAGCCTGATAATAAATTTAAACATCCAGAGCCTCCTGATGAGGAAGAGGAGGAGGAGTTGGACAAATTCATAGCTGAAATTGAGGATGCAGCGGAACGAGAGTGGGCTGCGGAGGAAGCAGCTGAGAAAGAGGAGTTCACCAGAATGAGGTATTGGAACAGAGAGGAGTACGGTGGGAGATACAGAAGATCTGAAGAACCTGAACATGACAATTCTGATGATGAGATCCCGAGGGCAAGGGATTGGAGGCATACACATGGCAAGCAGAGGCCTAATGGTGATTACGAAGATGACGATGAGGAATGGCTTTCTAGTAATGTTGTGGGCTCTAGTGTTCTCGACAGTGATGCTGATGATTCTGATGGAACGCCTGAGAAGTTCAAGATATCTCCAAGAGACTGGGGGAGGCAGGATAAGATTGGCAGGGCGAAGAATGATGAATGCTTTTATAGAAATTCTGAAGCTAACAATAGAAAAGCTAATAATAGAAAGATTATGGTGGACGATGGCACTGAATCAGAAAATGTGCTGAGCGATCTTGATACTGCAATGTGGAAATTCAATTCTGAGGAAGAGCACGACTCAACAGCATCAAGAGCTGCGAACTATGATTTCCGAAGTAGCAGTGATGAAGAAGAGGACTTAACATACACAAAGGGAGGAAAGGAGTGGGTAAATGATTCCGAAAGCAACACTGTTTATTCCAACAAAGCTCGTGGCAAGTTTGAGGCATCAAGAGTTGCAGAGGGGAAACACGATAGGAGGATTGGCAGGGGCAACAGTGAGTATTTCAAAAGAAATGCTGATGTTAGCACAAGAAGAAAGCCGGTTGAGGAGGATGTCAAGTCCAAGGATATCTCCGGTGATTCTGAAAACGAAATATGGGAATTAAACGCTGCCAGCGACTCTGGAGCATCGACAGGGAGAATCAATTACAACAGCAGCGACGAAGAGGATTATCAAGTGAGGAGGGAAGAGAAGAAAGGAAATGGCAATAAAAGCACAAGAACAAGAAAAGACTTGGATGAGGATTGGGACAGTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000077 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0006974 GO:0008150 GO:0009314 GO:0009628 GO:0009889 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010564 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031570 GO:0033043 GO:0033554 GO:0040020 GO:0043565 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051445 GO:0051716 GO:0051726 GO:0051783 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2000241 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRS1_YhbY

Protein Analysis

673

Amino Acids

77.13

Weight (kDa)

6.73

Isoelectric Point (pI)

53.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRS1_YhbY	PF01985	152 - 234	2.4e-14	CRS1 / YhbY (CRM) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27550 AT3G27550 AT3G27550
fragaria_vesca	FvH4_6g51690
malus_domestica	MD09G1023500.v1.1 MD17G1023000.v1.1
prunus_persica	Prupe.3G293300_v2.0.a1
pyrus_communis	pycom111g01960 pycom17g01970
rosa_chinensis	RchiOBHm_Chr2g0173221
rosa_laevigata	RLG00000022171
rosa_multiflora	Rmu_sc0000860.1_g000003 Rmu_sc0039142.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079110
rosa_rugosa	Rorug02G0566600
rosa_samantha	Rh2AG646500 Rh2BG657200 Rh2CG622400 Rh2DG670800
rosa_wichuraiana	Rw2G053100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	522, 702
AciI	CCGC	7 cut(s)	42, 253, 522, 702, 838, 1064, 1082
AclWI	GGATC	5 cut(s)	199, 431, 857, 870, 1192
AcsI	RAATTY	5 cut(s)	727, 986, 1037, 1426, 1529
AcuI	CTGAAG	4 cut(s)	110, 449, 1185, 1452
AfaI	GTAC	4 cut(s)	123, 404, 582, 1142
AfiI	CCNNNNNNNGG	3 cut(s)	188, 189, 1119
AhdI	GACNNNNNGTC	1 cut(s)	961
AjuI	GAANNNNNNNTTGG	2 cut(s)	1108, 1140
AloI	GAACNNNNNNTCC	2 cut(s)	819, 851
Alw21I	GWGCWC	1 cut(s)	1551
Alw26I	GTCTC	5 cut(s)	100, 163, 569, 637, 1362
AlwI	GGATC	5 cut(s)	199, 431, 857, 870, 1192
AlwNI	CAGNNNCTG	1 cut(s)	977
Ama87I	CYCGRG	2 cut(s)	455, 1201
AoxI	GGCC	2 cut(s)	627, 1241
ApoI	RAATTY	5 cut(s)	727, 986, 1037, 1426, 1529
AseI	ATTAAT	1 cut(s)	852
AsuHPI	GGTGA	4 cut(s)	417, 1102, 1262, 1847
AvaI	CYCGRG	2 cut(s)	455, 1201
AxyI	CCTNAGG	1 cut(s)	858
BamHI	GGATCC	1 cut(s)	862
BanII	GRGCYC	1 cut(s)	1301
BauI	CACGAG	2 cut(s)	572, 1686
BbsI	GAAGAC	1 cut(s)	937
Bbv12I	GWGCWC	1 cut(s)	1551
BbvI	GCAGC	9 cut(s)	472, 730, 844, 1066, 1073, 1103, 1558, 1855, 1926
BccI	CCATC	3 cut(s)	182, 1328, 1472
BcgI	CGANNNNNNTGC	2 cut(s)	1301, 1335
BclI	TGATCA	1 cut(s)	919
BcoDI	GTCTC	5 cut(s)	100, 163, 569, 637, 1362
BfaI	CTAG	5 cut(s)	198, 216, 446, 1284, 1302
BfmI	CTRYAG	1 cut(s)	966
BglII	AGATCT	2 cut(s)	415, 1160
BlpI	GCTNAGC	2 cut(s)	249, 1502
BmeRI	GACNNNNNGTC	1 cut(s)	961
BmeT110I	CYCGRG	2 cut(s)	455, 1201
BmiI	GGNNCC	1 cut(s)	864
BmrI	ACTGGG	1 cut(s)	1381
BmsI	GCATC	8 cut(s)	246, 562, 891, 1048, 1309, 1571, 1712, 1895
BmuI	ACTGGG	1 cut(s)	1381
BoxI	GACNNNNGTC	1 cut(s)	933
BpiI	GAAGAC	1 cut(s)	937
BplI	GAGNNNNNCTC	2 cut(s)	1538, 1570
BpmI	CTGGAG	1 cut(s)	1902
Bpu10I	CCTNAGC	1 cut(s)	441
Bpu1102I	GCTNAGC	2 cut(s)	249, 1502
Bsa29I	ATCGAT	1 cut(s)	203
BsaI	GGTCTC	1 cut(s)	163
BsaJI	CCNNGG	3 cut(s)	949, 1202, 1821
BsaWI	WCCGGW	1 cut(s)	1832
BsaXI	ACNNNNNCTCC	6 cut(s)	525, 555, 819, 849, 1013, 1043
Bsc4I	CCNNNNNNNGG	3 cut(s)	188, 189, 1119
Bse118I	RCCGGY	2 cut(s)	714, 1798
Bse1I	ACTGG	3 cut(s)	566, 866, 1376
Bse21I	CCTNAGG	1 cut(s)	858
Bse3DI	GCAATG	3 cut(s)	124, 873, 1527
BseCI	ATCGAT	1 cut(s)	203
BseDI	CCNNGG	3 cut(s)	949, 1202, 1821
BseGI	GGATG	6 cut(s)	261, 882, 994, 1063, 1816, 2002
BseLI	CCNNNNNNNGG	3 cut(s)	188, 189, 1119
BseMI	GCAATG	3 cut(s)	124, 873, 1527
BseMII	CTCAG	8 cut(s)	432, 638, 815, 849, 1086, 1335, 1493, 1530
BseNI	ACTGG	3 cut(s)	566, 866, 1376
BseXI	GCAGC	9 cut(s)	472, 730, 844, 1066, 1073, 1103, 1558, 1855, 1926
Bsh1236I	CGCG	2 cut(s)	522, 702
BshFI	GGCC	2 cut(s)	629, 1243
BshVI	ATCGAT	1 cut(s)	203
BsiHKAI	GWGCWC	1 cut(s)	1551
BsiHKCI	CYCGRG	2 cut(s)	455, 1201
BsiSI	CCGG	3 cut(s)	715, 1799, 1833
BslFI	GGGAC	1 cut(s)	968
BslI	CCNNNNNNNGG	3 cut(s)	188, 189, 1119
BsmAI	GTCTC	5 cut(s)	100, 163, 569, 637, 1362
BsmFI	GGGAC	1 cut(s)	968
BsmI	GAATGC	1 cut(s)	1418
BsnI	GGCC	2 cut(s)	629, 1243
Bso31I	GGTCTC	1 cut(s)	163
BsoBI	CYCGRG	2 cut(s)	455, 1201
Bsp1286I	GDGCHC	2 cut(s)	1301, 1551
Bsp1407I	TGTACA	1 cut(s)	580
Bsp1720I	GCTNAGC	2 cut(s)	249, 1502
Bsp19I	CCATGG	1 cut(s)	949
BspACI	CCGC	7 cut(s)	42, 253, 522, 702, 838, 1064, 1082
BspANI	GGCC	2 cut(s)	629, 1243
BspCNI	CTCAG	8 cut(s)	433, 639, 814, 850, 1087, 1336, 1494, 1531
BspDI	ATCGAT	1 cut(s)	203
BspFNI	CGCG	2 cut(s)	522, 702
BspHI	TCATGA	1 cut(s)	433
BspLI	GGNNCC	1 cut(s)	864
BspPI	GGATC	5 cut(s)	199, 431, 857, 870, 1192
BspQI	GCTCTTC	1 cut(s)	1539
BspTNI	GGTCTC	1 cut(s)	163
BsrDI	GCAATG	3 cut(s)	124, 873, 1527
BsrFI	RCCGGY	2 cut(s)	714, 1798
BsrGI	TGTACA	1 cut(s)	580
BsrI	ACTGG	3 cut(s)	566, 866, 1376
BssAI	RCCGGY	2 cut(s)	714, 1798
BssECI	CCNNGG	3 cut(s)	949, 1202, 1821
BssSI	CACGAG	2 cut(s)	572, 1686
BssT1I	CCWWGG	2 cut(s)	949, 1821
Bst2BI	CACGAG	2 cut(s)	572, 1686
Bst4CI	ACNGT	7 cut(s)	580, 963, 1145, 1316, 1669, 1754, 2012
Bst6I	CTCTTC	7 cut(s)	52, 163, 1011, 1539, 1602, 1917, 1941
BstAUI	TGTACA	1 cut(s)	580
BstC8I	GCNNGC	3 cut(s)	880, 1235, 1873
BstDSI	CCRYGG	1 cut(s)	949
BstF5I	GGATG	6 cut(s)	261, 882, 994, 1063, 1816, 2002
BstFNI	CGCG	2 cut(s)	522, 702
BstMAI	GTCTC	5 cut(s)	100, 163, 569, 637, 1362
BstMWI	GCNNNNNNNGC	6 cut(s)	136, 237, 391, 1088, 1568, 1971
BstPAI	GACNNNNGTC	1 cut(s)	933
BstSFI	CTRYAG	1 cut(s)	966
BstUI	CGCG	2 cut(s)	522, 702
BstV1I	GCAGC	9 cut(s)	472, 730, 844, 1066, 1073, 1103, 1558, 1855, 1926
BstV2I	GAAGAC	1 cut(s)	937
BstX2I	RGATCY	4 cut(s)	415, 862, 1160, 1197
BstXI	CCANNNNNNTGG	1 cut(s)	1371
BstYI	RGATCY	4 cut(s)	415, 862, 1160, 1197
Bsu15I	ATCGAT	1 cut(s)	203
Bsu36I	CCTNAGG	1 cut(s)	858
BsuRI	GGCC	2 cut(s)	629, 1243
BsuTUI	ATCGAT	1 cut(s)	203
BtgI	CCRYGG	1 cut(s)	949
BtsCI	GGATG	6 cut(s)	261, 882, 994, 1063, 1816, 2002
BtsI	GCAGTG	1 cut(s)	1603
BtsIMutI	CAGTG	7 cut(s)	559, 1321, 1482, 1603, 1665, 1759, 2017
Cac8I	GCNNGC	3 cut(s)	880, 1235, 1873
CaiI	CAGNNNCTG	1 cut(s)	977
CciI	TCATGA	1 cut(s)	433
Cfr10I	RCCGGY	2 cut(s)	714, 1798
ClaI	ATCGAT	1 cut(s)	203
Csp6I	GTAC	4 cut(s)	122, 403, 581, 1141
CspCI	CAANNNNNGTGG	2 cut(s)	1669, 1704
CviAII	CATG	6 cut(s)	232, 434, 694, 950, 1178, 1229
CviQI	GTAC	4 cut(s)	122, 403, 581, 1141
DraI	TTTAAA	1 cut(s)	991
DriI	GACNNNNNGTC	1 cut(s)	961
Eam1104I	CTCTTC	7 cut(s)	52, 163, 1011, 1539, 1602, 1917, 1941
Eam1105I	GACNNNNNGTC	1 cut(s)	961
EarI	CTCTTC	7 cut(s)	52, 163, 1011, 1539, 1602, 1917, 1941
EciI	GGCGGA	2 cut(s)	31, 827
Eco130I	CCWWGG	2 cut(s)	949, 1821
Eco147I	AGGCCT	1 cut(s)	1243
Eco24I	GRGCYC	1 cut(s)	1301
Eco31I	GGTCTC	1 cut(s)	163
Eco32I	GATATC	2 cut(s)	1359, 1828
Eco57I	CTGAAG	4 cut(s)	110, 449, 1185, 1452
Eco81I	CCTNAGG	1 cut(s)	858
Eco88I	CYCGRG	2 cut(s)	455, 1201
EcoRV	GATATC	2 cut(s)	1359, 1828
EcoT14I	CCWWGG	2 cut(s)	949, 1821
EcoT22I	ATGCAT	2 cut(s)	555, 916
EcoT38I	GRGCYC	1 cut(s)	1301
ErhI	CCWWGG	2 cut(s)	949, 1821
FaeI	CATG	6 cut(s)	235, 437, 697, 953, 1181, 1232
FalI	AAGNNNNNCTT	2 cut(s)	470, 502
FaqI	GGGAC	1 cut(s)	968
FatI	CATG	6 cut(s)	231, 433, 693, 949, 1177, 1228
FauI	CCCGC	1 cut(s)	515
FbaI	TGATCA	1 cut(s)	919
FokI	GGATG	6 cut(s)	268, 869, 981, 1070, 1823, 2009
FriOI	GRGCYC	1 cut(s)	1301
FspBI	CTAG	5 cut(s)	198, 216, 446, 1284, 1302
GsuI	CTGGAG	1 cut(s)	1902
HaeIII	GGCC	2 cut(s)	629, 1243
HapII	CCGG	3 cut(s)	715, 1799, 1833
Hin1II	CATG	6 cut(s)	235, 437, 697, 953, 1181, 1232
HindIII	AAGCTT	1 cut(s)	305
HpaII	CCGG	3 cut(s)	715, 1799, 1833
HphI	GGTGA	4 cut(s)	417, 1102, 1262, 1847
Hpy166II	GTNNAC	3 cut(s)	667, 1110, 1474
Hpy8I	GTNNAC	3 cut(s)	667, 1110, 1474
Hpy99I	CGWCG	1 cut(s)	1922
HpyAV	CCTTC	4 cut(s)	97, 193, 754, 919
HpyCH4III	ACNGT	7 cut(s)	580, 963, 1145, 1316, 1669, 1754, 2012
HpyCH4V	TGCA	7 cut(s)	259, 553, 878, 914, 1061, 1520, 1715
HpyF10VI	GCNNNNNNNGC	6 cut(s)	136, 237, 391, 1088, 1568, 1971
Hsp92II	CATG	6 cut(s)	235, 437, 697, 953, 1181, 1232
Ksp22I	TGATCA	1 cut(s)	919
LguI	GCTCTTC	1 cut(s)	1539
LmnI	GCTCC	3 cut(s)	237, 840, 1883
Lsp1109I	GCAGC	9 cut(s)	472, 730, 844, 1066, 1073, 1103, 1558, 1855, 1926
LweI	GCATC	8 cut(s)	246, 562, 891, 1048, 1309, 1571, 1712, 1895
MaeI	CTAG	5 cut(s)	198, 216, 446, 1284, 1302
MaeIII	GTNAC	2 cut(s)	143, 448
MflI	RGATCY	4 cut(s)	415, 862, 1160, 1197
MhlI	GDGCHC	2 cut(s)	1301, 1551
MlyI	GAGTC	4 cut(s)	271, 275, 1547, 1871
MmeI	TCCRAC	2 cut(s)	1011, 1701
Mph1103I	ATGCAT	2 cut(s)	555, 916
MseI	TTAA	7 cut(s)	86, 731, 852, 909, 990, 1616, 1862
MslI	CAYNNNNRTG	2 cut(s)	1115, 1227
MspA1I	CMGCKG	2 cut(s)	1064, 1094
MspI	CCGG	3 cut(s)	715, 1799, 1833
Mva1269I	GAATGC	1 cut(s)	1418
MvnI	CGCG	2 cut(s)	522, 702
MwoI	GCNNNNNNNGC	6 cut(s)	136, 237, 391, 1088, 1568, 1971
NcoI	CCATGG	1 cut(s)	949
NlaIII	CATG	6 cut(s)	235, 437, 697, 953, 1181, 1232
NlaIV	GGNNCC	1 cut(s)	864
NmeAIII	GCCGAG	2 cut(s)	77, 165
NmuCI	GTSAC	1 cut(s)	143
NsiI	ATGCAT	2 cut(s)	555, 916
PaeR7I	CTCGAG	1 cut(s)	455
PagI	TCATGA	1 cut(s)	433
PceI	AGGCCT	1 cut(s)	1243
PciSI	GCTCTTC	1 cut(s)	1539
PcsI	WCGNNNNNNNCGW	2 cut(s)	1263, 1872
PctI	GAATGC	1 cut(s)	1418
PfeI	GAWTC	6 cut(s)	797, 1328, 1487, 1652, 1838, 1899
PleI	GAGTC	4 cut(s)	271, 274, 1547, 1871
PpsI	GAGTC	4 cut(s)	271, 274, 1547, 1871
PshAI	GACNNNNGTC	1 cut(s)	933
PshBI	ATTAAT	1 cut(s)	852
PspN4I	GGNNCC	1 cut(s)	864
PspXI	VCTCGAGB	1 cut(s)	455
PstNI	CAGNNNCTG	1 cut(s)	977
PsuI	RGATCY	4 cut(s)	415, 862, 1160, 1197
PvuII	CAGCTG	1 cut(s)	1094
RsaI	GTAC	4 cut(s)	123, 404, 582, 1142
RsaNI	GTAC	4 cut(s)	122, 403, 581, 1141
RseI	CAYNNNNRTG	2 cut(s)	1115, 1227
SapI	GCTCTTC	1 cut(s)	1539
SaqAI	TTAA	7 cut(s)	86, 731, 852, 909, 990, 1616, 1862
SchI	GAGTC	4 cut(s)	271, 275, 1547, 1871
SduI	GDGCHC	2 cut(s)	1301, 1551
SfaNI	GCATC	8 cut(s)	246, 562, 891, 1048, 1309, 1571, 1712, 1895
SfcI	CTRYAG	1 cut(s)	966
Sfr274I	CTCGAG	1 cut(s)	455
SlaI	CTCGAG	1 cut(s)	455
SmiMI	CAYNNNNRTG	2 cut(s)	1115, 1227
SmlI	CTYRAG	1 cut(s)	455
SmoI	CTYRAG	1 cut(s)	455
SseBI	AGGCCT	1 cut(s)	1243
SsiI	CCGC	7 cut(s)	42, 253, 522, 702, 838, 1064, 1082
SspMI	CTAG	5 cut(s)	198, 216, 446, 1284, 1302
StuI	AGGCCT	1 cut(s)	1243
StyI	CCWWGG	2 cut(s)	949, 1821
TaaI	ACNGT	7 cut(s)	580, 963, 1145, 1316, 1669, 1754, 2012
TaqI	TCGA	7 cut(s)	167, 186, 203, 456, 795, 1311, 1889
TaqII	GACCGA	1 cut(s)	341
TatI	WGTACW	2 cut(s)	121, 580
TfiI	GAWTC	6 cut(s)	797, 1328, 1487, 1652, 1838, 1899
Tru1I	TTAA	7 cut(s)	86, 731, 852, 909, 990, 1616, 1862
Tru9I	TTAA	7 cut(s)	86, 731, 852, 909, 990, 1616, 1862
TscAI	CASTG	7 cut(s)	566, 1321, 1489, 1603, 1672, 1759, 2017
TseFI	GTSAC	1 cut(s)	143
Tsp45I	GTSAC	1 cut(s)	143
TspDTI	ATGAA	4 cut(s)	153, 1030, 1425, 1617
TspGWI	ACGGA	1 cut(s)	945
TspRI	CASTG	7 cut(s)	566, 1321, 1489, 1603, 1672, 1759, 2017
VspI	ATTAAT	1 cut(s)	852
XapI	RAATTY	5 cut(s)	727, 986, 1037, 1426, 1529
XbaI	TCTAGA	2 cut(s)	197, 215
XhoI	CTCGAG	1 cut(s)	455
XspI	CTAG	5 cut(s)	198, 216, 446, 1284, 1302
Zsp2I	ATGCAT	2 cut(s)	555, 916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.