pycom17g03650

Pyridoxamine 5'-phosphate oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0006888
Physical Location & Seq
Reverse (-)
2440939 .. 2443165
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
ATGACGATGACGACGATGATCAAAGGGCTTGTTCACTTCGTGTTCGCAGTCTACTTGGTTTCTCTACTGTCGGGTATCCAACTGCCTGTGGATGGGCGGGTTCTCTTGCTGAAGAAACCCGACCCGGAAAATGCTGCCGCCACTGCTCGTTGGTTGGTCGCTCAGAATTCCTGGGGCGTCTTGAATACTATCTCAAGTGAATTGGGAGGAGCACCCTTCGGGAATGTGGTTTCATTTAGCGATGGAGAACCTGGAAATGGCCGTGGTATCCCTTACTTCTACTTGACAACTCTTGATCCGACTGCAAGAAACGCATTGAATGATCCAAGAGCTTCGTTGACAATTAGTGAGCATCCTATTGGAACCTGCGGCAAGAGAGACCCTGAGAATCCCACTTGTGCAAAGCTTACACTTAACGGGAAGCTGAAGCTTGCGGATCCCAAAGAAACAGAATTTGCTATAAGTGCCTTGTTCACAAAGCATCCGGAGATGAAGGACTGGCCCAAGGATCACAACTTCCAGTTCTTCAAATTAGACATCGAGAATATCTTTCTGATCGATTGGTTTGGCGGTCCAAAACCTCTCACAGTCGATCAGTACCTTCATCTCAAAACGACAGAGAACGCCTTGATTCGAGGATTACTCGTGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pyrid_oxidase_2

Protein Analysis

218

Amino Acids

23.94

Weight (kDa)

6.51

Isoelectric Point (pI)

17.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CREG_beta-barrel PF13883 40 - 201 2.6e-60 CREG, beta-barrel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 374
AccI GTMKAC 1 cut(s) 51
AccIII TCCGGA 1 cut(s) 484
AciI CCGC 5 cut(s) 97, 138, 369, 434, 570
AclWI GGATC 5 cut(s) 290, 317, 431, 444, 516
AcoI YGGCCR 1 cut(s) 259
AcsI RAATTY 2 cut(s) 166, 452
AcuI CTGAAG 2 cut(s) 131, 446
AcyI GRCGYC 1 cut(s) 177
AdeI CACNNNGTG 1 cut(s) 40
AfaI GTAC 1 cut(s) 599
AfiI CCNNNNNNNGG 2 cut(s) 92, 257
AgsI TTSAA 3 cut(s) 184, 319, 529
AjnI CCWGG 2 cut(s) 170, 250
AluBI AGCT 4 cut(s) 332, 406, 424, 430
AluI AGCT 4 cut(s) 332, 406, 424, 430
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 1 cut(s) 372
AlwI GGATC 5 cut(s) 290, 317, 431, 444, 516
Aor13HI TCCGGA 1 cut(s) 484
AoxI GGCC 2 cut(s) 259, 500
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 2 cut(s) 166, 452
AspS9I GGNCC 2 cut(s) 501, 572
AsuC2I CCSGG 1 cut(s) 125
AvaII GGWCC 1 cut(s) 572
BamHI GGATCC 1 cut(s) 436
BauI CACGAG 1 cut(s) 644
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 1 cut(s) 121
BccI CCATC 2 cut(s) 86, 236
BceAI ACGGC 1 cut(s) 246
BciT130I CCWGG 2 cut(s) 172, 252
BciVI GTATCC 2 cut(s) 86, 278
BclI TGATCA 1 cut(s) 18
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 374
BfuI GTATCC 2 cut(s) 86, 278
BisI GCNGC 3 cut(s) 135, 138, 370
BlsI GCNGC 3 cut(s) 136, 139, 371
Bme1390I CCNGG 3 cut(s) 125, 172, 252
Bme18I GGWCC 1 cut(s) 572
BmgT120I GGNCC 2 cut(s) 501, 572
BmiI GGNNCC 2 cut(s) 364, 438
BmrFI CCNGG 3 cut(s) 125, 172, 252
BmsI GCATC 2 cut(s) 361, 490
BplI GAGNNNNNCTC 1 cut(s) 627
BpuEI CTTGAG 1 cut(s) 178
BpuMI CCSGG 1 cut(s) 125
Bsa29I ATCGAT 1 cut(s) 558
BsaHI GRCGYC 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 372
BsaJI CCNNGG 3 cut(s) 171, 262, 504
BsaWI WCCGGW 1 cut(s) 484
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 257
Bse1I ACTGG 2 cut(s) 503, 520
BseAI TCCGGA 1 cut(s) 484
BseBI CCWGG 2 cut(s) 172, 252
BseCI ATCGAT 1 cut(s) 558
BseDI CCNNGG 3 cut(s) 171, 262, 504
BseGI GGATG 3 cut(s) 97, 352, 481
BseLI CCNNNNNNNGG 2 cut(s) 92, 257
BseMII CTCAG 2 cut(s) 176, 375
BseNI ACTGG 2 cut(s) 503, 520
BseRI GAGGAG 1 cut(s) 222
BseXI GCAGC 1 cut(s) 121
BshFI GGCC 2 cut(s) 261, 502
BshVI ATCGAT 1 cut(s) 558
BsiHKAI GWGCWC 1 cut(s) 214
BsiSI CCGG 2 cut(s) 125, 485
BslI CCNNNNNNNGG 2 cut(s) 92, 257
BsmAI GTCTC 1 cut(s) 372
BsnI GGCC 2 cut(s) 261, 502
Bso31I GGTCTC 1 cut(s) 372
Bsp1286I GDGCHC 1 cut(s) 214
Bsp13I TCCGGA 1 cut(s) 484
Bsp143I GATC 8 cut(s) 18, 295, 322, 436, 508, 555, 592, 648
BspACI CCGC 5 cut(s) 97, 138, 369, 434, 570
BspANI GGCC 2 cut(s) 261, 502
BspCNI CTCAG 2 cut(s) 175, 376
BspDI ATCGAT 1 cut(s) 558
BspEI TCCGGA 1 cut(s) 484
BspLI GGNNCC 2 cut(s) 364, 438
BspMI ACCTGC 1 cut(s) 374
BspPI GGATC 5 cut(s) 290, 317, 431, 444, 516
BspTNI GGTCTC 1 cut(s) 372
BsrI ACTGG 2 cut(s) 503, 520
BssECI CCNNGG 3 cut(s) 171, 262, 504
BssMI GATC 8 cut(s) 18, 295, 322, 436, 508, 555, 592, 648
BssNI GRCGYC 1 cut(s) 177
BssSI CACGAG 1 cut(s) 644
BssT1I CCWWGG 1 cut(s) 504
Bst2BI CACGAG 1 cut(s) 644
Bst2UI CCWGG 2 cut(s) 172, 252
Bst4CI ACNGT 2 cut(s) 69, 589
BstACI GRCGYC 1 cut(s) 177
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 2 cut(s) 162, 384
BstDSI CCRYGG 1 cut(s) 262
BstF5I GGATG 3 cut(s) 97, 352, 481
BstKTI GATC 8 cut(s) 21, 298, 325, 439, 511, 558, 595, 651
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 8 cut(s) 18, 295, 322, 436, 508, 555, 592, 648
BstMWI GCNNNNNNNGC 3 cut(s) 143, 311, 464
BstNI CCWGG 2 cut(s) 172, 252
BstSCI CCNGG 3 cut(s) 123, 170, 250
BstV1I GCAGC 1 cut(s) 121
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
Bsu15I ATCGAT 1 cut(s) 558
BsuI GTATCC 2 cut(s) 86, 278
BsuRI GGCC 2 cut(s) 261, 502
BsuTUI ATCGAT 1 cut(s) 558
BtgI CCRYGG 1 cut(s) 262
BtgZI GCGATG 1 cut(s) 255
BtsCI GGATG 3 cut(s) 97, 352, 481
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 1 cut(s) 141
BveI ACCTGC 1 cut(s) 374
Cac8I GCNNGC 1 cut(s) 432
Cfr13I GGNCC 2 cut(s) 501, 572
ClaI ATCGAT 1 cut(s) 558
CseI GACGC 1 cut(s) 166
Csp6I GTAC 1 cut(s) 598
CviJI RGCY 7 cut(s) 28, 261, 332, 406, 424, 430, 502
CviKI_1 RGCY 7 cut(s) 28, 261, 332, 406, 424, 430, 502
CviQI GTAC 1 cut(s) 598
DdeI CTNAG 2 cut(s) 162, 384
DpnI GATC 8 cut(s) 20, 297, 324, 438, 510, 557, 594, 650
DpnII GATC 8 cut(s) 18, 295, 322, 436, 508, 555, 592, 648
DraIII CACNNNGTG 1 cut(s) 40
EaeI YGGCCR 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 504
Eco31I GGTCTC 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 572
Eco57I CTGAAG 2 cut(s) 131, 446
EcoRI GAATTC 1 cut(s) 166
EcoRII CCWGG 2 cut(s) 170, 250
EcoT14I CCWWGG 1 cut(s) 504
ErhI CCWWGG 1 cut(s) 504
FaiI YATR 1 cut(s) 461
FauI CCCGC 1 cut(s) 90
FbaI TGATCA 1 cut(s) 18
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 3 cut(s) 135, 138, 370
FokI GGATG 3 cut(s) 104, 339, 468
Fsp4HI GCNGC 3 cut(s) 135, 138, 370
GluI GCNGC 3 cut(s) 135, 138, 370
HaeIII GGCC 2 cut(s) 261, 502
HapII CCGG 2 cut(s) 125, 485
HgaI GACGC 1 cut(s) 166
Hin1I GRCGYC 1 cut(s) 177
HincII GTYRAC 1 cut(s) 339
HindII GTYRAC 1 cut(s) 339
HindIII AAGCTT 2 cut(s) 404, 428
HinfI GANTC 2 cut(s) 388, 631
HpaII CCGG 2 cut(s) 125, 485
Hpy166II GTNNAC 4 cut(s) 34, 52, 339, 474
Hpy188I TCNGA 3 cut(s) 165, 300, 555
Hpy188III TCNNGA 6 cut(s) 181, 220, 293, 485, 541, 646
Hpy8I GTNNAC 4 cut(s) 34, 52, 339, 474
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 3 cut(s) 226, 487, 611
HpyCH4III ACNGT 2 cut(s) 69, 589
HpyCH4V TGCA 2 cut(s) 305, 401
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 311, 464
HpyF3I CTNAG 2 cut(s) 162, 384
Hsp92I GRCGYC 1 cut(s) 177
Kpn2I TCCGGA 1 cut(s) 484
Ksp22I TGATCA 1 cut(s) 18
Kzo9I GATC 8 cut(s) 18, 295, 322, 436, 508, 555, 592, 648
LmnI GCTCC 1 cut(s) 209
Lsp1109I GCAGC 1 cut(s) 121
LweI GCATC 2 cut(s) 361, 490
MalI GATC 8 cut(s) 20, 297, 324, 438, 510, 557, 594, 650
MboI GATC 8 cut(s) 18, 295, 322, 436, 508, 555, 592, 648
MboII GAAGA 2 cut(s) 124, 517
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 5 cut(s) 166, 200, 342, 452, 530
MmeI TCCRAC 2 cut(s) 103, 323
MnlI CCTC 3 cut(s) 200, 591, 629
MroI TCCGGA 1 cut(s) 484
MseI TTAA 1 cut(s) 414
MspI CCGG 2 cut(s) 125, 485
MspR9I CCNGG 3 cut(s) 125, 172, 252
MvaI CCWGG 2 cut(s) 172, 252
MwoI GCNNNNNNNGC 3 cut(s) 143, 311, 464
NciI CCSGG 1 cut(s) 125
NdeII GATC 8 cut(s) 18, 295, 322, 436, 508, 555, 592, 648
NlaIV GGNNCC 2 cut(s) 364, 438
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PfeI GAWTC 2 cut(s) 388, 631
PkrI GCNGC 3 cut(s) 136, 139, 371
Psp6I CCWGG 2 cut(s) 170, 250
PspGI CCWGG 2 cut(s) 170, 250
PspN4I GGNNCC 2 cut(s) 364, 438
PspPI GGNCC 2 cut(s) 501, 572
PsuI RGATCY 1 cut(s) 436
RsaI GTAC 1 cut(s) 599
RsaNI GTAC 1 cut(s) 598
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 3 cut(s) 135, 138, 370
Sau3AI GATC 8 cut(s) 18, 295, 322, 436, 508, 555, 592, 648
Sau96I GGNCC 2 cut(s) 501, 572
ScrFI CCNGG 3 cut(s) 125, 172, 252
SduI GDGCHC 1 cut(s) 214
SetI ASST 8 cut(s) 253, 334, 368, 408, 426, 432, 583, 603
SfaNI GCATC 2 cut(s) 361, 490
SinI GGWCC 1 cut(s) 572
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
Sse9I AATT 5 cut(s) 166, 200, 342, 452, 530
SsiI CCGC 5 cut(s) 97, 138, 369, 434, 570
StyD4I CCNGG 3 cut(s) 123, 170, 250
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 2 cut(s) 69, 589
TaqI TCGA 4 cut(s) 540, 558, 591, 634
TasI AATT 5 cut(s) 166, 200, 342, 452, 530
TauI GCSGC 2 cut(s) 140, 372
TfiI GAWTC 2 cut(s) 388, 631
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 1 cut(s) 148
TseI GCWGC 1 cut(s) 134
TspDTI ATGAA 3 cut(s) 222, 506, 593
TspRI CASTG 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 572
XapI RAATTY 2 cut(s) 166, 452
XmiI GTMKAC 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.