Rh2CG600300

Pyridoxamine 5'-phosphate oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0006888
Physical Location & Seq
Forward (+)
77578048 .. 77580792
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG600300.1

Sequence Viewer

Length: 519 bp
ATGTTTGCCTCAGGATCTTGTATCTTTACTTGTTTATTTGTTACTGCAACTCCTACGATGTTTCTGTATCAAATTGATCATCAAATACAATACAGGAATGTGGTATCATTTAGTGATGGGGAACCTGGAAAAGGAAGTGGTATCCCTTACTTCTACTTGACAACTCTTGATCCGACTGCAAGAAATGCACTGAAAGACCAAAGGGCTTCTTTGACAATTAGTGAGCATCCTATTGGAACCTGTGGCAAAGTTGACCCTGAAAACCCCACCTGTGCAAAGATTACACTTACAGGAAAGTTGAAGATTGCTAATGAAGGGCCCAAAGAACAAGAAATTGCTAGAAAGGCCTTGTTCTCAAAGCATCCAGAGATGAAAGACTGGCCCAAGGGTCACAACTTCAAGTTCTTCAAGTTAGACATCGAGGATATCTTTTTGATCGATTGGTTTGGGGGTCCAAAACCTCTCACAGTGGACCAATACCTTCAAGCCAAATTGACAGAACCTGCCTTCATTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pyrid_oxidase_2

Protein Analysis

172

Amino Acids

19.31

Weight (kDa)

6.89

Isoelectric Point (pI)

19.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CREG_beta-barrel PF13883 29 - 161 5.6e-48 CREG, beta-barrel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 511
AclWI GGATC 2 cut(s) 22, 164
AfiI CCNNNNNNNGG 1 cut(s) 131
AgsI TTSAA 4 cut(s) 301, 400, 409, 485
AjnI CCWGG 1 cut(s) 124
AlwI GGATC 2 cut(s) 22, 164
AlwNI CAGNNNCTG 1 cut(s) 503
AoxI GGCC 3 cut(s) 317, 345, 380
ApaI GGGCCC 1 cut(s) 321
AspS9I GGNCC 5 cut(s) 317, 318, 381, 452, 472
AvaII GGWCC 2 cut(s) 452, 472
AxyI CCTNAGG 1 cut(s) 10
BaeGI GKGCMC 1 cut(s) 321
BanII GRGCYC 1 cut(s) 321
BccI CCATC 1 cut(s) 110
BciT130I CCWGG 1 cut(s) 126
BciVI GTATCC 1 cut(s) 152
BclI TGATCA 1 cut(s) 76
BfaI CTAG 1 cut(s) 339
BfuAI ACCTGC 1 cut(s) 511
BfuI GTATCC 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 126
Bme18I GGWCC 2 cut(s) 452, 472
BmgT120I GGNCC 5 cut(s) 317, 318, 381, 452, 472
BmiI GGNNCC 4 cut(s) 123, 238, 319, 453
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 2 cut(s) 235, 370
Bsa29I ATCGAT 1 cut(s) 438
BsaJI CCNNGG 1 cut(s) 384
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse1I ACTGG 1 cut(s) 383
Bse21I CCTNAGG 1 cut(s) 10
BseBI CCWGG 1 cut(s) 126
BseCI ATCGAT 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 384
BseGI GGATG 2 cut(s) 226, 361
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 383
BseSI GKGCMC 1 cut(s) 321
BshFI GGCC 3 cut(s) 319, 347, 382
BshVI ATCGAT 1 cut(s) 438
BslI CCNNNNNNNGG 1 cut(s) 131
BsnI GGCC 3 cut(s) 319, 347, 382
Bsp120I GGGCCC 1 cut(s) 317
Bsp1286I GDGCHC 1 cut(s) 321
Bsp143I GATC 4 cut(s) 14, 76, 169, 435
BspANI GGCC 3 cut(s) 319, 347, 382
BspCNI CTCAG 1 cut(s) 23
BspDI ATCGAT 1 cut(s) 438
BspLI GGNNCC 4 cut(s) 123, 238, 319, 453
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 2 cut(s) 22, 164
BsrI ACTGG 1 cut(s) 383
BssECI CCNNGG 1 cut(s) 384
BssMI GATC 4 cut(s) 14, 76, 169, 435
BssT1I CCWWGG 1 cut(s) 384
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 469
BstAPI GCANNNNNTGC 1 cut(s) 185
BstDEI CTNAG 1 cut(s) 10
BstENI CCTNNNNNAGG 1 cut(s) 129
BstF5I GGATG 2 cut(s) 226, 361
BstKTI GATC 4 cut(s) 17, 79, 172, 438
BstMBI GATC 4 cut(s) 14, 76, 169, 435
BstMWI GCNNNNNNNGC 2 cut(s) 185, 344
BstNI CCWGG 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 124
BstSLI GKGCMC 1 cut(s) 321
BstX2I RGATCY 1 cut(s) 14
BstYI RGATCY 1 cut(s) 14
Bsu15I ATCGAT 1 cut(s) 438
Bsu36I CCTNAGG 1 cut(s) 10
BsuI GTATCC 1 cut(s) 152
BsuRI GGCC 3 cut(s) 319, 347, 382
BsuTUI ATCGAT 1 cut(s) 438
BtsCI GGATG 2 cut(s) 226, 361
BtsIMutI CAGTG 2 cut(s) 188, 474
BveI ACCTGC 1 cut(s) 511
CaiI CAGNNNCTG 1 cut(s) 503
Cfr13I GGNCC 5 cut(s) 317, 318, 381, 452, 472
ClaI ATCGAT 1 cut(s) 438
CviJI RGCY 5 cut(s) 206, 319, 347, 382, 488
CviKI_1 RGCY 5 cut(s) 206, 319, 347, 382, 488
DdeI CTNAG 1 cut(s) 10
DpnI GATC 4 cut(s) 16, 78, 171, 437
DpnII GATC 4 cut(s) 14, 76, 169, 435
Eco130I CCWWGG 1 cut(s) 384
Eco147I AGGCCT 1 cut(s) 347
Eco24I GRGCYC 1 cut(s) 321
Eco32I GATATC 1 cut(s) 427
Eco47I GGWCC 2 cut(s) 452, 472
Eco81I CCTNAGG 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 129
EcoO109I RGGNCCY 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 124
EcoRV GATATC 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 384
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 1 cut(s) 384
FalI AAGNNNNNCTT 2 cut(s) 193, 225
FbaI TGATCA 1 cut(s) 76
FokI GGATG 2 cut(s) 213, 348
FriOI GRGCYC 1 cut(s) 321
FspBI CTAG 1 cut(s) 339
HaeIII GGCC 3 cut(s) 319, 347, 382
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
Hpy166II GTNNAC 2 cut(s) 253, 472
Hpy188I TCNGA 2 cut(s) 174, 518
Hpy188III TCNNGA 3 cut(s) 12, 167, 365
Hpy8I GTNNAC 2 cut(s) 253, 472
HpyAV CCTTC 3 cut(s) 308, 491, 517
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4V TGCA 4 cut(s) 47, 179, 188, 275
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 344
HpyF3I CTNAG 1 cut(s) 10
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 4 cut(s) 14, 76, 169, 435
LpnPI CCDG 9 cut(s) 79, 111, 138, 253, 270, 276, 283, 364, 378
LweI GCATC 2 cut(s) 235, 370
MaeI CTAG 1 cut(s) 339
MaeIII GTNAC 2 cut(s) 40, 389
MalI GATC 4 cut(s) 16, 78, 171, 437
MboI GATC 4 cut(s) 14, 76, 169, 435
MboII GAAGA 2 cut(s) 313, 397
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 4 cut(s) 72, 216, 333, 491
MmeI TCCRAC 1 cut(s) 197
MnlI CCTC 3 cut(s) 19, 415, 471
MspR9I CCNGG 1 cut(s) 126
MvaI CCWGG 1 cut(s) 126
MwoI GCNNNNNNNGC 2 cut(s) 185, 344
NdeII GATC 4 cut(s) 14, 76, 169, 435
NlaIV GGNNCC 4 cut(s) 123, 238, 319, 453
NmuCI GTSAC 1 cut(s) 389
PceI AGGCCT 1 cut(s) 347
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 4 cut(s) 123, 238, 319, 453
PspOMI GGGCCC 1 cut(s) 317
PspPI GGNCC 5 cut(s) 317, 318, 381, 452, 472
PstNI CAGNNNCTG 1 cut(s) 503
PsuI RGATCY 1 cut(s) 14
Sau3AI GATC 4 cut(s) 14, 76, 169, 435
Sau96I GGNCC 5 cut(s) 317, 318, 381, 452, 472
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 1 cut(s) 321
SetI ASST 6 cut(s) 127, 242, 272, 463, 483, 505
SfaNI GCATC 2 cut(s) 235, 370
SinI GGWCC 2 cut(s) 452, 472
Sse9I AATT 4 cut(s) 72, 216, 333, 491
SseBI AGGCCT 1 cut(s) 347
SspMI CTAG 1 cut(s) 339
StuI AGGCCT 1 cut(s) 347
StyD4I CCNGG 1 cut(s) 124
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 1 cut(s) 469
TaqI TCGA 2 cut(s) 420, 438
TasI AATT 4 cut(s) 72, 216, 333, 491
TscAI CASTG 2 cut(s) 195, 474
TseFI GTSAC 1 cut(s) 389
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 3 cut(s) 327, 386, 499
TspRI CASTG 2 cut(s) 195, 474
VpaK11BI GGWCC 2 cut(s) 452, 472
XagI CCTNNNNNAGG 1 cut(s) 129
XspI CTAG 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.